Hemorrhagic septicemia is an acute, deadly disease of cattle and buffaloes associated with colossal economic loss in the livestock industry in the Asian regions particularly Malaysia. Therefore, this study was conducted to investigate on the Polymerase chain reaction detection of Pasteurella multocida type B: 2 in mice inoculated through different routes with river water contaminated with infected mice carcasses. Sixty five mice were used for the study; five mice were placed in each tank containing river water for 24, 48 and 72 h. The groups comprise of five mice each made up of the control, intraperitoneal, oral and the aerosol routes. A dose of 1 mL 109 CFU of Pasteurella multocida type B: 2 obtained from the infected river water were inoculated into each group intraperitoneally and the aerosol route while, 0.4 mL of 109 CFU of Pasteurella multocida type B: 2 was inoculated orally into the group. The control group was inoculated with 1 mL buffer saline pH 7. The PCR results in the present ...
TY - JOUR. T1 - Wound infection with Neisseria weaveri and a novel subspecies of pasteurella multocida in a child who sustained a tiger bite.. AU - Capitini, Christian M.. AU - Herrero, Inmaculada A.. AU - Patel, Robin. AU - Ishitani, Michael B.. AU - Boyce, Thomas G.. PY - 2002. Y1 - 2002. N2 - A 7-year-old girl developed a wound infection as a result of a tiger bite she sustained. DNA sequence analysis revealed that the causative organisms were Neisseria weaveri and what is, to our knowledge, a previously undescribed subspecies of Pasteurella multocida, for which we propose the designation "Pasteurella multocida subspecies tigris subspecies nov.". AB - A 7-year-old girl developed a wound infection as a result of a tiger bite she sustained. DNA sequence analysis revealed that the causative organisms were Neisseria weaveri and what is, to our knowledge, a previously undescribed subspecies of Pasteurella multocida, for which we propose the designation "Pasteurella multocida subspecies tigris ...
Development of a rapid multiplex PCR assay to genotype pasteurella multocida strains by use of the lipopolysaccharide outer core biosynthesis locus
A reporter plasmid pSRG has been developed which expresses red fluorescent protein (RFP) from a constitutive prokaryotic promoter within Pasteurella multocida B:2 and green fluorescent protein (GFP) from a constitutive eukaryotic promoter within mammalian cells. This construct has been used to determine the location and viability of the bacteria when moving from the extracellular environment into the intracellular compartment of mammalian cells. Invasion assays with embryonic bovine lung (EBL) cells and an attenuated AroA- derivative of Pasteurella multocida B:2 (strain JRMT12), harbouring the plasmid pSRG, showed that RFP-expressing bacteria could be detected intracellularly at 3 h post-invasion. At this stage, some EBL cells harbouring RFP-expressing bacteria were observed to express GFP simultaneously, indicating release of the plasmid into the intracellular environment. At 5 h post-invasion, more EBL cells were expressing GFP, while still harbouring RFP-expressing bacteria. Concurrently, some EBL
TY - JOUR. T1 - Virulence gene profiling and ompA sequence analysis of Pasteurella multocida and their correlation with host species. AU - Ujvári, B.. AU - Makrai, L.. AU - Magyar, T.. PY - 2019/6/1. Y1 - 2019/6/1. N2 - This study describes the prevalence of capsule biosynthesis genes, LPS genotypes, virulence associated genes and the analysis of the outer membrane protein (ompA)sequence of Pasteurella multocida isolates (n = 180)from different locations in Hungary, from various host species, including humans. When combining capsular types with LPS genotypes, eight capsule - LPS genotype combinations were detected. A: L3 was the most dominant in bovine and porcine isolates, A: L1 in feline and human isolates, while D: L3 was the most common among strains from small ruminants. The P. multocida toxin encoding gene toxA was highly prevalent among small ruminant and porcine strains, while in human, feline and bovine isolates it could not be detected. Combination of the tested virulence associated ...
The first tetracycline resistance (tet) gene of hybridization class H was detected in 1993 on plasmid pVM111 (4) from a Pasteurella multocida isolate obtained in 1975 from the tissues of a turkey in California that had died of avian cholera (5). Later, tet(H) genes were also detected in porcine and bovine P. multocida and Mannheimia haemolytica isolates (3). In 1998, the tet(H) gene was identified as part of the composite transposon Tn5706 from P. multocida (12). In recent years, three types of tet(H)-carrying plasmids, designated pPMT1 (12), pPAT1 (8), and pMHT1 (7), have been analyzed in detail. All these plasmids were detected in either P. multocida, Pasteurella aerogenes, or various Mannheimia sp. isolates from cattle or pigs. They were 4.4 to 6.8 kb in size and mediated only tetracycline resistance. While restriction maps and sequence data for the regions flanking the tetR-tet(H) gene region were available for these plasmids, the corresponding data are still missing for pVM111. Since ...
An outbreak of avian cholera was observed among wild birds in a few localities in Denmark in 2001. The highest mortalities were among breeding ciders (Somateria mollissima) and gulls (Larus spp.). Pulsed-field gel electrophoresis (PFGE) was conducted using ApaI and SmaI as restriction enzymes and restriction enzyme analysis (REA) using HpaII. The Pasteurella multocida subsp. multocida strain isolated from birds in this outbreak was indistinguishable from a strain that caused outbreaks in 1996 and 2003. Most isolates from domestic poultry had other PFGE patterns but some were indistinguishable from the outbreak strain. Among 68 isolates from wild birds, only one PFGE and one REA pattern were demonstrated, whereas among 23 isolates from domestic poultry, 14 different SmaI, 12 different ApaI, and 10 different HpaII patterns were found. The results suggest that a P. multocida strain has survived during several years among wild birds in Denmark ...
Pasteurella multocida (PM) can cause progressive atrophic rhinitis and suppurative bronchopneumonia in pigs. The present study performed antimicrobial susceptibility testing and serotype and genotype identification on the 62 PM strains isolated from the lungs of diseased pigs with respiratory sympto
BioAssay record AID 132241 submitted by ChEMBL: Effective dose required to inhibit Pasteurella multocida infection model in mouse by subcutaneous administration; ND means not determined.
Pasteurella pneumotropica is an opportunistic pathogen that is not often associated with clinical diseases. However, when infecting a host, it can generally be recovered from the respiratory tract, the urogenital tract, or conjunctiva from the host: common hosts include mice, rats, hamsters, guinea pigs, rabbits, cats, and other laboratory animals. In the case of humans, many strains from Pasteurella multocida subsp. multocida, Pasteurella multocida subsp. septica, Pasteurella canis, Pasteurella stomatis, and Pasteurella dogmatis have been isolated from infected humans. Toxins were produced only by one strain of P. multocida subsp. multocida and P. canis; in addition, other than one severe case of necrotizing cellulitits caused by P. dagmatis, P. multocida subsp. multocida or P. multocida subsp. septica was involved in the more serious cases of infection. Symptoms of a Pasteurella infection vary on which body organ is involved and how long the disease is present. One of the most common symptoms ...
The broad host-range cloning vectors, pJRD215 and pMMB67EH, were evaluated for stability and cloning efficiency in Pasteurella multocida. Transformation of P. multocida by electroporation was unreliable and poorly efficient regardless of whether the transforming DNA was isolated from E. coli or P. multocida. Both vectors contain a mob site that enabled transfer by conjugation from E. coli to P. multocida with high efficiency. Kanamycin, streptomycin, and ampicillin resistance encoded by the vectors were expressed in P. multocida. LacZ was cloned in pMMB67EH, an expression vector, and was transferred to P. multocida by conjugation. The transconjugants expressed a functional beta-galactosidase as determined by o-nitrophenyl-beta-D-galactopyranoside (ONPG) test. We propose the use of these cosmid and expression vectors as a shuttle vectors for cloning in P. multocida.
Phosphocholine (PCho) is an important substituent of surface structures expressed by a number of bacterial pathogens. Its role in virulence has been investigated in several species, in which it has been shown to play a role in bacterial adhesion to mucosal surfaces, in resistance to antimicrobial peptides, or in sensitivity to complement-mediated killing. The lipopolysaccharide (LPS) structure of Pasteurella multocida strain Pm70, whose genome sequence is known, has recently been determined and does not contain PCho. However, LPS structures from the closely related, virulent P. multocida strains VP161 and X-73 were shown to contain PCho on their terminal galactose sugar residues. To determine if PCho was involved in the virulence of P. multocida, we used subtractive hybridization of the VP161 genome against the Pm70 genome to identify a four-gene locus (designated pcgDABC) which we show is required for the addition of the PCho residues to LPS. The proteins predicted to be encoded by pcgABC ...
Domain combinations containing the Carbohydrate phosphatase superfamily in Pasteurella multocida subsp. multocida str. Pm70. Domain architectures illustrate each occurrence of the Carbohydrate phosphatase superfamily.
... Alignments can be refined by adding alignments from other genomes, adding your own sequences and/or aligning to other models from the same superfamily. The display of alignments can also be customised.
Summary: The effects of subminimal inhibitory concentrations (subMICs) of certain antibiotics, namely penicillin G, tetracycline and trimethoprim/sulphamethoxazole, on the growth and morphology of Pasteurella multocida were evaluated. SubMICs of penicillin markedly reduced the growth of P. multocida. Tetracycline and trimethoprim/ sulphamethoxazole had no effect on its growth. SubMICs of penicillin greatly affected the morphology of P. multocida. At the highest concentrations tested (1/2 and 1/4 MIC) cells were acapsulate, and long filamentous cells (4-6 μm) were observed with some isolates. There was no correlation between the observed differences in the penicillin-binding proteins of the P. multocida isolates, and the extent of cell filamentation induced by penicillin G. SubMICs of tetracycline and trimethoprim/sulphamethoxazole did not seem to affect capsule production although filamentation was observed. Our results indicate that subMICs of penicillin can reduce growth of P. multocida. Furthermore,
Definition of Pasteurella multocida with photos and pictures, translations, sample usage, and additional links for more information.
TY - JOUR. T1 - Community-acquired bacteremic pneumonia due to Pasteurella multocida subspecies multocida in a patient with poor-control diabetes mellitus. AU - Lee, Wen Sen. AU - Chen, Fu Lun. AU - Wang, Cheng Hui. AU - Ou, Tsong Yih. AU - Lin, Yi Hsuan. AU - Jean, Shio Shin. PY - 2019/2/1. Y1 - 2019/2/1. UR - http://www.scopus.com/inward/record.url?scp=85039155987&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=85039155987&partnerID=8YFLogxK. U2 - 10.1016/j.jmii.2017.11.006. DO - 10.1016/j.jmii.2017.11.006. M3 - Article. AN - SCOPUS:85039155987. VL - 52. SP - 163. EP - 164. JO - Journal of Microbiology, Immunology and Infection. JF - Journal of Microbiology, Immunology and Infection. SN - 0253-2662. IS - 1. ER - ...
International Journal of Genomics is a peer-reviewed, Open Access journal that publishes research articles as well as review articles in all areas of genome-scale analysis. Topics covered by the journal include, but are not limited to: bioinformatics, clinical genomics, disease genomics, epigenomics, evolutionary genomics, functional genomics, genome engineering, and synthetic genomics.
Pasteurella multocida is the most common respiratory pathogen in the domestic rabbit. Infection with the bacteria can result in rhinitis, conjunctivitis, pneumonia, abscesses, genital tract infections, and septicemia. The bacteria can be transmitted via aerosol or contact with an infected animal, either directly or through fomites. P. multocida initially colonizes the pharynx, then moves to the nasal cavity and surrounding tissue, with the potential to spread to the rest of the body. Colonization of the nasal cavity may take two weeks to occur, at which time clinical signs may or may not appear. Infected rabbits may become carriers without exhibiting clinical signs. ...
Investigations with antisera prepared in rabbits and sheep and active immunity experiments in mice showed that strains of Pasteurella multocida which, according to the haemagglutination test, are serologically related, do not necessarily give rise to cross immunity. Conversely, it was found that serologically unrelated strains may exhibit an appreciable degree of cross protection. The unreliability of the haemagglutination test as an indicator of immunological identity of strains was further confirmed in that peritoneal fluid from immunized mice showed passive protection properties despite the absence of haemagglutinating antibodies. There was no consistent agreement between the results obtained with antisera prepared in different species and active immunity experiments in mice, and this leads to the conclusion that different species probably vary quantitatively and qualitatively in their immune response to immunization with P. multocida. Attempts to determine whether combined bacterins afforded ...
Citation: Brockmeier, S., Register, K.B. 2007. Expression of the dermonecrotic toxin by Bordetella bronchiseptica is not necessary for predisposing to infection with toxigenic Pasteurella multocida. Veterinary Microbiology. 125(3-4):284-289. Interpretive Summary: Infection with Bordetella bronchiseptica predisposes pigs to infection with Pasteurella multocida, and coinfection with these agents is often found in cases of progressive atrophic rhinitis, a disease which causes deformed growth of the pigs snout. There are several potential mechanisms by which B. bronchiseptica could predispose to infection with P. multocida. B. bronchiseptica produces a toxin called the dermonecrotic toxin, or DNT, which induces damage to swine nasal tissue and causes pneumonia and could play a role in increased colonization by P. multocida. This experiment was designed to determine whether a strain of B. bronchiseptica which does not produce DNT is still capable of predisposing pigs to infection with P. multocida. ...
Wehmann E, Khayer B, Magyar T (2015) Heterogeneity of Bordetella bronchiseptica adenylate cyclase (cyaA) RTX domain. Arch Microbiol. 197(1): 105-112. doi: 10.1007/s00203-014-1068-x. Magyar T, Donkó T, Repa I, Kovács M (2013) Regeneration of toxigenic Pasteurella multocida induced severe turbinate atrophy in pigs detected by computed tomography. BMC Vet Res. 9: 222. doi: 10.1186/1746-6148-9-222. Sellyei B, Wehmann E, Magyar T (2012) Sequencing-independent method for the differentiation of the main phylogenetic lineages of Pasteurella multocida. J Vet Diagn Invest. 24(4): 735-738. doi: 10.1177/1040638712447794. Pósa R, Donkó T, Bogner P, Kovács M, Repa I, Magyar T (2011) Interaction of Bordetella bronchiseptica, Pasteurella multocida, and fumonisin B1 in the porcine respiratory tract as studied by computed tomography. Can J Vet Res. 75(3): 176-182.. Sellyei B, Wehmann E, Makrai L, Magyar T (2011) Evaluation of the Biolog system for the identification of certain closely related Pasteurella ...
Isolation, identification and antimicrobial susceptibility of Pasteurella multocida from cattle with hemorrhagic septicemia in Assosa and Bambasi districts
Identification of novel glycosyltransferases required for assembly of the Pasteurella multocida A:1 lipopolysaccharide and their involvement in virulence
BioAssay record AID 155766 submitted by ChEMBL: Antibacterial activity against Pasteurella multocida (an important animal respiratory pathogen)..
Fox, R R.; Norberg, R F.; and Myers, D D., " The relationship of Pasteurella multocida to otitis media in the domestic rabbit (Oryctolagus cuniculus)." (1971). Faculty Research 1970 - 1979. 165 ...
Infeksi bakteri Pasteurella multocida dapat menyebabkan penyakit pernafasan yang parah, umumnya ditandai dengan antara lain infeksi hidung, sinusitis, infeksi telinga, konjungtivitis, radang paru-paru, dan infeksi umum dari darah. Kondisi ini sering disebut sebagai ingusan karena napas mendengus membuat kelinci terkena dampak suara. Hal ini juga dapat menyebabkan abses di subkutan (di bawah lapisan atas kulit)…
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YAKIMA - State and federal wildlife-diagnostic centers have confirmed an outbreak of avian cholera near the Tri-Cities, where more than 1,200 dead ducks have been reported in the past week. The disease was confirmed in dead ducks found near Burbank, Wash., and tested by the USGS National Wildlife Health Center and the Washington Animal Disease Diagnostic Lab. The birds tested negative for avian influenza, a different disease fatal to waterfowl and other birds.. Avian cholera is caused by the bacterium Pasteurella multocida and is one of the most common diseases among ducks, geese and other wild North American waterfowl, said Katie Haman, a wildlife veterinarian at the Washington Department of Fish and Wildlife (WDFW). "Humans are not at a high risk for infection with the bacterial strain causing avian cholera, though infections in humans are possible," Haman said. "We advise people to avoid handling sick or dead birds, and to report any they find.". Reports can be filed online ...
in dead ducks found near the Tri-Cities. YAKIMA State and federal wildlife-diagnostic centers have confirmed an outbreak of avian cholera near the Tri-Cities, where more than 1,200 dead ducks have been reported in the past week.. The disease was confirmed in dead ducks found near Burbank, Wash., and tested by the USGS National Wildlife Health Center and the Washington Animal Disease Diagnostic Lab. The birds tested negative for avian influenza, a different disease fatal to waterfowl and other birds.. Avian cholera is caused by the bacterium Pasteurella multocida and is one of the most common diseases among ducks, geese and other wild North American waterfowl, said Katie Haman, a wildlife veterinarian at the Washington Department of Fish and Wildlife (WDFW).. "Humans are not at a high risk for infection with the bacterial strain causing avian cholera, though infections in humans are possible," Haman said. "We advise people to avoid handling sick or dead birds, and to report any they ...
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The citrate cycle (TCA cycle, Krebs cycle) is an important aerobic pathway for the final steps of the oxidation of carbohydrates and fatty acids. The cycle starts with acetyl-CoA, the activated form of acetate, derived from glycolysis and pyruvate oxidation for carbohydrates and from beta oxidation of fatty acids. The two-carbon acetyl group in acetyl-CoA is transferred to the four-carbon compound of oxaloacetate to form the six-carbon compound of citrate. In a series of reactions two carbons in citrate are oxidized to CO2 and the reaction pathway supplies NADH for use in the oxidative phosphorylation and other metabolic processes. The pathway also supplies important precursor metabolites including 2-oxoglutarate. At the end of the cycle the remaining four-carbon part is transformed back to oxaloacetate. According to the genome sequence data, many organisms seem to lack genes for the full cycle [MD:M00009], but contain genes for specific segments [MD:M00010 M00011 ...
Cat bite fever is caused by pasteurella multocida. Pasteurella multocida is gram negative bipolar staining rod. The most common mode of transmission of pasteurella multocida is via the bites from the cats.
Abstract BACKGROUND: SP-A and SP-D are hydrophilic proteins which regulate the inflammatory response of the lung. Pasteurella multocida is one of the most common bacteria isolated from calves suffering from shipping fever pneumonia, one of the most problems in dairy herds. OBJECTIVE: Evaluation of surfactant content may provide a valuable diagnostic tool for detection of calf pneumonia due to Pasteurella multocida and also state of treatment. METHODS: Ten Holstein-Frisian bull calves aged 4 months with body weight of 120 ± 5 kg were selected for study in two groups. The Pasteurella multocida (PMC66 Razi) was used in the present study for inducing pneumonia. The Bronchoalveolar lavage (BAL) process was done in selected calves. BAL fluid was collected and centrifuged and finally the sediment (crude surfactant) was reserved at -20˚C.The cytological evaluation and surfactant content was assayed by ELISA, TPL kit assay and HPLC. RESULTS: The serum levels of SP-A and SP-D in pneumonic group were
Adhesion of type A Pasteurella mulocida to rabbit pharyngeal cells and its possible role in rabbit respiratory tract infections.: Pasteurella multocida serotype
Pasteurella multocida (G-ve, non-motile, non-spore forming, rod 0.2 -0.4 µm by 0.6 - 2.5 µm, aerobic or anaerobic). Classified according to capsular antigens (A, B, D, E and F) by a passive haemaggluitnation test and the tube agglutination or preciptin tests to detect the somatic antigens (serovars 1 to 16). A multiplex capsular PCR assay can now recognise all these serotypes. All except capsular antigen E and somatic antigens 8 and 13 have been found in birds. Capsular antigen A with somatic antigens 3 and 4 are most commonly reconised in the UK and USA associated with virulent Fowl Cholera. ...
Diancourt et al. The population structure of Acinetobacter baumannii: expanding multiresistant clones from an ancestral susceptible genetic pool. PLoS One 2010, 7:e10034. The Escherichia coli scheme #1 (Achtman scheme) samples the following genes: adk; fumC; gyrB; icd; mdh; purA; recA. and is described in:. Wirth et al. Sex and virulence in Escherichia coli: an evolutionary perspective. Mol. Microbiol. 2006: 60(5); 1136-1151. The Escherichia coli scheme #2 (Pasteur scheme) samples the following genes: dinB; icdA; pabB; polB; putP; trpA; trpB; uidA. and is described in:. Jaureguy et al. Phylogenetic and genomic diversity of human bacteremic Escherichia coli strains. 2008. BMC Genomics 9:560. The Pasteurella multocida scheme #1 (RIRDC scheme) samples the following genes:. adk; est; pmi; zwf; mdh; gdh; pgi. and was developed by Robert Davies, University of Glasgow, UK.. The Pasteurella multocida scheme #2 (multihost scheme) samples the following genes:. adk; aroA; deoD; gdhA; g6pd; mdh; pgi. and ...
Protein GrpE; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP- dependent [...] (197 aa ...
in dead ducks found near the Tri-Cities. YAKIMA State and federal wildlife-diagnostic centers have confirmed an outbreak of avian cholera near the Tri-Cities, where more than 1,200 dead ducks have been reported in the past week.. The disease was confirmed in dead ducks found near Burbank, Wash., and tested by the USGS National Wildlife Health Center and the Washington Animal Disease Diagnostic Lab. The birds tested negative for avian influenza, a different disease fatal to waterfowl and other birds.. Avian cholera is caused by the bacterium Pasteurella multocida and is one of the most common diseases among ducks, geese and other wild North American waterfowl, said Katie Haman, a wildlife veterinarian at the Washington Department of Fish and Wildlife (WDFW).. "Humans are not at a high risk for infection with the bacterial strain causing avian cholera, though infections in humans are possible," Haman said. "We advise people to avoid handling sick or dead birds, and to report any they ...
Pasteurella multocida locally isolated from chicken and ducks (BCC 299, BCC 2331, DY1, DY2, 12TG, 15TG) andimported strains (BCC 1359, 1362; HEDDLESTON group 1 and 6 respectively) had been tested for its pathogenicity in theprevious study. The aims of this experiment were to study the preparation of local isolate pasteurellosis vaccines and to determine the protective effect of that vaccines in chicken against the highly pathogenic local isolates of P. multocida. Killed monovalent, bivalent and polyvalent pasteurellosis vaccines were prepared and each was adjunvanted with aluminum hydroxide gel at a final concentration of 1.5% and the cell concentration was equal to the No 10 of MacFarland tube standard. Each of the vaccine prepared was used to vaccinated on a group of six week old of layer chicken (8 per group). Each chicken was subcutaneously injected with 0.2 ml of vaccine, four weeks later each was boostered with similar vaccine with the same dose. Two weeks after giving the boostered ...
Cytotoxic necrotizing factors from E. coli (CNF1, CNF2) and Yersinia (CNFy) share N-terminal sequence similarity with Pasteurella multocida toxin (PMT). This common N-terminal region harbors the receptor-binding and translocation domains that mediate uptake and delivery of the C-terminal catalytic cargo domains into the host cytosol. Subtle variations in the N-terminal ~500 amino acids of CNFs and PMT could allow for selective recognition of cellular receptors and thus, selective target cell specificity. Through studies with cellular inhibitors, we have identified an additional novel function for this region in modulating responses of these toxin proteins to changes in pH during intoxication and delivery of the catalytic cargo domain into the cytosol.
In the March 13 Proceedings of the National Academy of Science, May et al. report the complete sequence of the Pasteurella multocida (Pm70) genome (Proc Natl Acad Sci USA 2001, 98:3460-3465). P. multocida causes disease in birds, cattle, swine and humans, and has been studied ever since Pasteur used it in vaccine development, but the mechanisms underlying its virulence are unknown. May et al. used a shotgun strategy to sequence over 53,000 DNA fragments and assemble them into a single circular sequence of about 2.26 megabases. The Pm70 genome contains 2,014 predicted coding regions, accounting for 89% of the entire chromosome, as well as 6 rRNA operons and 57 tRNA genes. About 10% of the open reading frames are unique to P. multocida, while over half have orthologs found in closely-related Haemophilus influenza and in Escherichia coli. Comparative analysis suggests that P. multocida diverged from H. influenza around 230 million years ago and from E. coli around 680 million years ago. May et al. ...
Fowl cholera, attributable to Pasteurella multocida, continues to be a problem in meat-chicken-breeder operations and has emerged as a drawback for free-range meat chickens. Here, utilizing whole-genome sequencing (WGS) and phylogenomic evaluation, we examine isolate relatedness throughout outbreaks of fowl cholera on a free-range meat hen farm over a 5-year interval.. Our genomic evaluation revealed that whereas all outbreak isolates have been sequence kind (ST) 20, they could possibly be separated into two distinct clades (clade 1 and clade 2) according to distinction in their lipopolysaccharide (LPS) kind. The isolates from the earlier outbreaks (clade 1) have been carrying LPS kind L3 whereas these from the more moderen outbreaks (clade 2) have been LPS kind L1. Additionally, WGS information indicated excessive inter- and intra-chicken genetic variety throughout a single outbreak. Furthermore, we display that whereas a killed autogenous vaccine carrying LPS kind L3 had been profitable in ...
The primary objective of this randomized controlled challenge study was to investigate the effect of ampicillin on ultrasonographic (US) lung consolidation after experimental challenge with Pasteurella multocida in preweaned dairy calves. The secondary objectives were to determine whether ampicillin affected respiratory score, gross consolidation, or the detection of P. multocida in lung tissue at postmortem exam (PME). Holstein bull calves (n = 39) were transported to the University of Wisconsin-Madison School of Veterinary Medicine isolation facility at the mean (±SD) age of 52 ± 6 d. After a 7-d acclimation period, 30 calves were inoculated intratracheally with 1010 cfu of ampicillin-sensitive P. multocida. Lung US and respiratory scoring were performed 2, 6, 12, and 24 h post-challenge, then US once daily and respiratory scoring twice daily until d 14. Calves were randomized to receive ampicillin [n = 17, treatment (TX), 6.6 mg/kg i.m. once daily for 3 d] or placebo [n = 11, control (CON), ...
Figure 5: Average (± SEM) comparison between concentrations of Pasteurella multocida B:2 in different parts of the luminal gastrointestinal tracts of Group 2 following experimental exposure ...
Pulmo-Guard PH-M pasteurella vaccine recommended for vaccination of healthy cattle 60 days of age or older as an aid in the prevention of respiratory disease due to Mannheimia (Pasteurella) haemolytica and Pasteurella multocida. Pulmo Guard PHM contains a toxoid as well as cell associated antigens
Patterns of use by sector. The greatest volume of antibiotic use is in intensively farmed poultry (including broilers for meat and layers for eggs) and pigs. These animals are kept indoors at a high density, which promotes the rapid transmission of bacterial infections, primarily affecting the respiratory and intestinal tracts.. Feedlot cattle and dairy cows are the next group in terms of the amount of antibiotics used. Slaughter cattle are generally raised under extensive conditions on farms, and then sent to a feedlot for rounding off before going to the abattoir. Feedlot cattle are prone to respiratory disease, caused by Mannheimia (Pasteurella) haemolytica, Pasteurella multocida, Histophilus (Haemophilus) somni and Mycoplasma, and mastitis, usually caused by Staphylococcus aureus.. Other ruminants (sheep and goats) are extensively farmed, together with the bulk of the population of cattle in South Africa. The main source of food is veld grass, and the density levels are low. Extensively kept ...
1.. Waraich KK, Duggal A, Cutrona A.Pasteurella multocida septicemia in chemotherapy-induced neutropenic dairy farmer with lung cancer. Infectious Diseases in Clinical Practice 18: 216-218, No. 3, May 2010. Available from: URL: http://dx.doi.org/10.1097/ipc.0b013e3181c75401 - USA ...
COW-CALF Health Program. Calving (December 10-31). Newborn Calves. • Tag claves (individual calf id/record calving - birth date intervals). • Vision C-D/Tetanus (optional prevention of neonatal scour deaths). • BVD PI test calf (ear notch). • Ecolizer Oral Scour protection 10cc po.. • Bovimune (Hyperimmune Serum) 20cc sq. Pre-breeding. Calves (at 60 days or more of age and before breeding cows). • Bovishield Gold 5 - Pfizer or Titamium 5 or Vista 5 (Use CattleMaster 4 - Pfizer if bulls are with cows and possibly rebred). • Vision 7. • Pateurella hemolytics and Pasteurella multocida. • Calves can be revaccinated with IBR, BVD, P13, BRSV in 3 weeks if neccessary. Cows and Bulls (before breeding). • Titanium 5, Vista 5 or Bovishield Gold FP - Pfizer (prior to or at first handling of synchronization - 10-14 days prior to breeding; use CattleMast 4 if any cows are bred). • Lepto 5, Spirovacc (do not administer to bulls). Weaning (summer). Calves. • Bovishield Gold 5 - Pfizer ...