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The objective of the project is to reduce and control water losses in the city water supply system. The preferred bidder will be responsible for the upgrade, operation and maintenance of the water dis...
Hemorrhagic septicemia is an acute, deadly disease of cattle and buffaloes associated with colossal economic loss in the livestock industry in the Asian regions particularly Malaysia. Therefore, this study was conducted to investigate on the Polymerase chain reaction detection of Pasteurella multocida type B: 2 in mice inoculated through different routes with river water contaminated with infected mice carcasses. Sixty five mice were used for the study; five mice were placed in each tank containing river water for 24, 48 and 72 h. The groups comprise of five mice each made up of the control, intraperitoneal, oral and the aerosol routes. A dose of 1 mL 109 CFU of Pasteurella multocida type B: 2 obtained from the infected river water were inoculated into each group intraperitoneally and the aerosol route while, 0.4 mL of 109 CFU of Pasteurella multocida type B: 2 was inoculated orally into the group. The control group was inoculated with 1 mL buffer saline pH 7. The PCR results in the present ...
BioAssay record AID 132241 submitted by ChEMBL: Effective dose required to inhibit Pasteurella multocida infection model in mouse by subcutaneous administration; ND means not determined.
Pasteurella pneumotropica is an opportunistic pathogen that is not often associated with clinical diseases. However, when infecting a host, it can generally be recovered from the respiratory tract, the urogenital tract, or conjunctiva from the host: common hosts include mice, rats, hamsters, guinea pigs, rabbits, cats, and other laboratory animals. In the case of humans, many strains from Pasteurella multocida subsp. multocida, Pasteurella multocida subsp. septica, Pasteurella canis, Pasteurella stomatis, and Pasteurella dogmatis have been isolated from infected humans. Toxins were produced only by one strain of P. multocida subsp. multocida and P. canis; in addition, other than one severe case of necrotizing cellulitits caused by P. dagmatis, P. multocida subsp. multocida or P. multocida subsp. septica was involved in the more serious cases of infection. Symptoms of a Pasteurella infection vary on which body organ is involved and how long the disease is present. One of the most common symptoms ...
TY - JOUR. T1 - Hydrogen peroxide as an effective disinfectant for Pasteurella multocida. AU - Jung, In Soo. AU - Kim, Hyun Jung. AU - Jung, Won Yong. AU - Kim, Chan Wha. PY - 2014/7. Y1 - 2014/7. N2 - Pasteurella multocida (P. multocida) infections vary widely, from local infections resulting from animal bites and scratches to general infections. As of yet, no vaccine against P. multocida has been developed, and the most effective way to prevent pathogenic transmission is to clean the host environment using disinfectants. In this study, we identified which disinfectants most effectively inhibited environmental isolates of P. multocida. Three readily available disinfectants were compared: 3% hydrogen peroxide (HP), 70% isopropyl alcohol, and synthetic phenol. In suspension tests and zone inhibition tests, 3% HP was the most promising disinfectant against P. multocida.. AB - Pasteurella multocida (P. multocida) infections vary widely, from local infections resulting from animal bites and ...
Development of a rapid multiplex PCR assay to genotype pasteurella multocida strains by use of the lipopolysaccharide outer core biosynthesis locus
The role of interleukin-8 (IL-8) as a neutrophil chemoattractant in bovine pneumonic pasteurellosis was investigated by characterizing: (1) the expression of IL-8 in the lesions of pneumonic pasteurellosis, (2) the in vitro and in vivo effects of recombinant bovine IL-8 on neutrophil chemotaxis, and (3) the importance of IL-8 as a neutrophil chemoattractant in this disease. The expression of IL-8 in bovine pneumonic pasteurellosis was assessed by Northern analysis, in situ hybridization and enzyme-linked immunosorbent assay. The expression of IL-8 mRNA was elevated dramatically in lesional lung compared to non-lesional lung, viral pneumonia, and normal lung. In situ hybridization revealed intense IL-8 mRNA expression in alveolar macrophages and neutrophils, and milder expression in several other cell types. Bronchoalveolar lavage (BAL) fluid from lesional lung contained 16.06 ± 4.00 ng/ml IL-8, but lower levels were present in non-lesional lung, viral pneumonia, and normal lung (mean ...
Looking for Pasteurella dagmatis? Find out information about Pasteurella dagmatis. A genus of gram-negative, nonmotile, nonsporulating, facultatively anaerobic coccobacillary to rod-shaped bacteria which are parasitic and often pathogens... Explanation of Pasteurella dagmatis
Looking for Pasteurella species A? Find out information about Pasteurella species A. A genus of gram-negative, nonmotile, nonsporulating, facultatively anaerobic coccobacillary to rod-shaped bacteria which are parasitic and often pathogens... Explanation of Pasteurella species A
Pasteurella multocida (PM) can cause progressive atrophic rhinitis and suppurative bronchopneumonia in pigs. The present study performed antimicrobial susceptibility testing and serotype and genotype identification on the 62 PM strains isolated from the lungs of diseased pigs with respiratory sympto
TY - JOUR. T1 - Virulence gene profiling and ompA sequence analysis of Pasteurella multocida and their correlation with host species. AU - Ujvári, B.. AU - Makrai, L.. AU - Magyar, T.. PY - 2019/6/1. Y1 - 2019/6/1. N2 - This study describes the prevalence of capsule biosynthesis genes, LPS genotypes, virulence associated genes and the analysis of the outer membrane protein (ompA)sequence of Pasteurella multocida isolates (n = 180)from different locations in Hungary, from various host species, including humans. When combining capsular types with LPS genotypes, eight capsule - LPS genotype combinations were detected. A: L3 was the most dominant in bovine and porcine isolates, A: L1 in feline and human isolates, while D: L3 was the most common among strains from small ruminants. The P. multocida toxin encoding gene toxA was highly prevalent among small ruminant and porcine strains, while in human, feline and bovine isolates it could not be detected. Combination of the tested virulence associated ...
The first tetracycline resistance (tet) gene of hybridization class H was detected in 1993 on plasmid pVM111 (4) from a Pasteurella multocida isolate obtained in 1975 from the tissues of a turkey in California that had died of avian cholera (5). Later, tet(H) genes were also detected in porcine and bovine P. multocida and Mannheimia haemolytica isolates (3). In 1998, the tet(H) gene was identified as part of the composite transposon Tn5706 from P. multocida (12). In recent years, three types of tet(H)-carrying plasmids, designated pPMT1 (12), pPAT1 (8), and pMHT1 (7), have been analyzed in detail. All these plasmids were detected in either P. multocida, Pasteurella aerogenes, or various Mannheimia sp. isolates from cattle or pigs. They were 4.4 to 6.8 kb in size and mediated only tetracycline resistance. While restriction maps and sequence data for the regions flanking the tetR-tet(H) gene region were available for these plasmids, the corresponding data are still missing for pVM111. Since ...
Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low
Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low
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TY - JOUR. T1 - A multifunctional Pasteurella multocida sialyltransferase. T2 - A powerful tool for the synthesis of sialoside libraries. AU - Yu, Hai. AU - Chokhawala, Harshal. AU - Karpel, Rebekah. AU - Yu, Hui. AU - Wu, Bingyuan. AU - Zhang, Jianbo. AU - Zhang, Yingxin. AU - Jia, Qiang. AU - Chen, Xi. PY - 2005/12/21. Y1 - 2005/12/21. N2 - A multifunctional sialyltransferase has been cloned from Pasteurella multocida strain P-1059 and expressed in E. coli as a truncated C-terminal His6-tagged recombinant protein (tPm0188Ph). Biochemical studies indicate that the obtained protein is (1) an α2,3-sialyltransferase (main function), (2) an α2,6-sialyltransferase, (3) an α2,3-sialidase, and (4) an α2,3-trans-sialidase. The recombinant tPm0188Ph is a powerful tool in the synthesis of structurally diverse sialoside libraries due to its relaxed substrate specificity, high solubility, high expression level, and multifunctionality.. AB - A multifunctional sialyltransferase has been cloned from ...
Domain combinations containing the Carbohydrate phosphatase superfamily in Pasteurella multocida subsp. multocida str. Pm70. Domain architectures illustrate each occurrence of the Carbohydrate phosphatase superfamily.
PTS IIb component alignments in Pasteurella multocida subsp. multocida str. Pm70. Alignments can be refined by adding alignments from other genomes, adding your own sequences and/or aligning to other models from the same superfamily. The display of alignments can also be customised.
Phosphocholine (PCho) is an important substituent of surface structures expressed by a number of bacterial pathogens. Its role in virulence has been investigated in several species, in which it has been shown to play a role in bacterial adhesion to mucosal surfaces, in resistance to antimicrobial peptides, or in sensitivity to complement-mediated killing. The lipopolysaccharide (LPS) structure of Pasteurella multocida strain Pm70, whose genome sequence is known, has recently been determined and does not contain PCho. However, LPS structures from the closely related, virulent P. multocida strains VP161 and X-73 were shown to contain PCho on their terminal galactose sugar residues. To determine if PCho was involved in the virulence of P. multocida, we used subtractive hybridization of the VP161 genome against the Pm70 genome to identify a four-gene locus (designated pcgDABC) which we show is required for the addition of the PCho residues to LPS. The proteins predicted to be encoded by pcgABC ...
An outbreak of avian cholera was observed among wild birds in a few localities in Denmark in 2001. The highest mortalities were among breeding ciders (Somateria mollissima) and gulls (Larus spp.). Pulsed-field gel electrophoresis (PFGE) was conducted using ApaI and SmaI as restriction enzymes and restriction enzyme analysis (REA) using HpaII. The Pasteurella multocida subsp. multocida strain isolated from birds in this outbreak was indistinguishable from a strain that caused outbreaks in 1996 and 2003. Most isolates from domestic poultry had other PFGE patterns but some were indistinguishable from the outbreak strain. Among 68 isolates from wild birds, only one PFGE and one REA pattern were demonstrated, whereas among 23 isolates from domestic poultry, 14 different SmaI, 12 different ApaI, and 10 different HpaII patterns were found. The results suggest that a P. multocida strain has survived during several years among wild birds in Denmark ...
Pasteurella species isolated from the bovine respiratory tract and their antimicrobial sensitivity patterns.: Pasteurella haemolytica biotype A, serotype 1 (P h
Capsules from a range of pathogenic bacteria are the key determinants of virulency. The capsule hasbeen implicated in virulence of Pasteurella multocida. In this study a type-specific polymerase chain reaction(PCR) assay was used for capsular typing of 39 avian P. multocida isolates from Iran. The PCR amplified afragment of 1044 bp from all of tested isolates. It was found that all avian P. multocida isolates belonged tocapsular type A. The sequence alignment of the fragment showed a high similarity (|96%) with thepublished sequences of P. multocida hya gene in the Gene Bank. It was recognised that P. multocida capsulargroup A is the dominant cause of fowl cholera in Iran.
International Journal of Genomics is a peer-reviewed, Open Access journal that publishes research articles as well as review articles in all areas of genome-scale analysis. Topics covered by the journal include, but are not limited to: bioinformatics, clinical genomics, disease genomics, epigenomics, evolutionary genomics, functional genomics, genome engineering, and synthetic genomics.
Pasteurella species are a component of the normal oropharyngeal flora in canine animals. In humans, the skin and soft tissue are the most common sites for Pasteurella infection and, less commonly, the respiratory tract can also be affected. We report a case of pneumonia by Pasteurella canis in a chronic respiratory disease patient with a history of permanent contact with a dog.. ...
Pasteurella canis is a Gram-negative, nonmotile, penicillin-sensitive coccobacillus belonging to the Pasteurellaceae family. Bacteria from this family cause zoonotic infections in humans, which manifest themselves as skin or soft-tissue infections after an animal bite. It has been known to cause serious disease in immunocompromised patients. Pasteurella was first described around 1880 and thought to be associated with chicken cholera and hemorrhagic septicemia in animals. The genus was first cultured in 1885. In 1920, strains of Pasteurella were isolated and observed in human infections. Micrococcus gallicidus was the first scientific name used for these bacteria. For a short time, the bacteria were unofficially placed in different genera, such as Octopsis, Coccobacillus, and Eucystia. In 1887, genus Pasteurella was proposed to honor Louis Pasteur for his critical discoveries in the field of microbiology. Before molecular technology was available, species were often labeled based on staining ...
Definition of Pasteurella multocida with photos and pictures, translations, sample usage, and additional links for more information.
The broad host-range cloning vectors, pJRD215 and pMMB67EH, were evaluated for stability and cloning efficiency in Pasteurella multocida. Transformation of P. multocida by electroporation was unreliable and poorly efficient regardless of whether the transforming DNA was isolated from E. coli or P. multocida. Both vectors contain a mob site that enabled transfer by conjugation from E. coli to P. multocida with high efficiency. Kanamycin, streptomycin, and ampicillin resistance encoded by the vectors were expressed in P. multocida. LacZ was cloned in pMMB67EH, an expression vector, and was transferred to P. multocida by conjugation. The transconjugants expressed a functional beta-galactosidase as determined by o-nitrophenyl-beta-D-galactopyranoside (ONPG) test. We propose the use of these cosmid and expression vectors as a shuttle vectors for cloning in P. multocida.
Summary: The effects of subminimal inhibitory concentrations (subMICs) of certain antibiotics, namely penicillin G, tetracycline and trimethoprim/sulphamethoxazole, on the growth and morphology of Pasteurella multocida were evaluated. SubMICs of penicillin markedly reduced the growth of P. multocida. Tetracycline and trimethoprim/ sulphamethoxazole had no effect on its growth. SubMICs of penicillin greatly affected the morphology of P. multocida. At the highest concentrations tested (1/2 and 1/4 MIC) cells were acapsulate, and long filamentous cells (4-6 μm) were observed with some isolates. There was no correlation between the observed differences in the penicillin-binding proteins of the P. multocida isolates, and the extent of cell filamentation induced by penicillin G. SubMICs of tetracycline and trimethoprim/sulphamethoxazole did not seem to affect capsule production although filamentation was observed. Our results indicate that subMICs of penicillin can reduce growth of P. multocida. Furthermore,
TY - JOUR. T1 - Identification of novel immunogens in Pasteurella multocida. AU - Al-Hasani, Keith. AU - Boyce, John Dallas. AU - McCarl, Victoria. AU - Bottomley, Stephen Paul. AU - Wilkie, Ian W. AU - Adler, Ben. PY - 2007. Y1 - 2007. N2 - P. multocida is a Gram-negative pathogen responsible for causing diseases in animals of economic significance to livestock industries throughout the world. Current vaccines include bacterins, which provide only limited protection against homologous serotypes. Therefore there is a need for more effective vaccines to control diseases caused by P. multocida. As a step towards developing vaccines against fowl cholera, a genomics based approach was applied for the identification of novel immunogens. Results: Bioinformatics analysis of the P. multocida genome predicted 129 proteins as secreted, located in the outer membrane, or lipoproteins. 105 of the genes encoding these proteins were cloned and recombinant protein expressed in Escherichia coli. Polyclonal serum ...
Pasteurella multocida(P. multocida)is part of the normal gastrointestinal and upper respiratory flora of domestic and wild animals such as dogs, cats, rabbits, opossums, boars, tigers, lions and horses (1, 2). Transmission of P. multocidais usually through scratches and bites leading to infections of the soft tissue and skin (3,4). P. multocida bacteremia is rare, but occurs in individuals with major risk factors including chronic pulmonary disease, diabetes mellitus, end state renal disease (ESRD), and an immunocompromised state (3,4). Non traumatic infections of P. multocida in immunocompromised individuals,are more likely to require care in the intensive care unit (ICU), likely to occur in patients with severe comorbidities, and are likely to end in mortality (4). In this case, we present P. multocida bacteremia in an immunocompromised adult male with end stage kidney on hemodialysis.
Wehmann E, Khayer B, Magyar T (2015) Heterogeneity of Bordetella bronchiseptica adenylate cyclase (cyaA) RTX domain. Arch Microbiol. 197(1): 105-112. doi: 10.1007/s00203-014-1068-x. Magyar T, Donkó T, Repa I, Kovács M (2013) Regeneration of toxigenic Pasteurella multocida induced severe turbinate atrophy in pigs detected by computed tomography. BMC Vet Res. 9: 222. doi: 10.1186/1746-6148-9-222. Sellyei B, Wehmann E, Magyar T (2012) Sequencing-independent method for the differentiation of the main phylogenetic lineages of Pasteurella multocida. J Vet Diagn Invest. 24(4): 735-738. doi: 10.1177/1040638712447794. Pósa R, Donkó T, Bogner P, Kovács M, Repa I, Magyar T (2011) Interaction of Bordetella bronchiseptica, Pasteurella multocida, and fumonisin B1 in the porcine respiratory tract as studied by computed tomography. Can J Vet Res. 75(3): 176-182.. Sellyei B, Wehmann E, Makrai L, Magyar T (2011) Evaluation of the Biolog system for the identification of certain closely related Pasteurella ...
In 1878, Pasteurella multocida was discovered in birds infected with cholera. Then in 1880, Louis Pasteur isolated it. P. multocida is a small, gram-negative bacterium. It is non-motile coccobacillus and penicillin-sensitive. It can cause infections in humans, as a result of cat or dog bites and scratches. Mammals and birds have it as part of their normal respiratory microbiota and display infections. P. multocida live in the upper respiratory tract of many vertebrate hosts. These include cats, dogs, rabbits, cows, pigs, and fowl. The host species provides these bacteria with nutrients, and if the bacteria are present in an external environment, it is only temporary. This bacteria is located in a wide range of environments. Cholera outbreaks are usually reported in the United States in north central California, the MidWest, and the Muleshoe National Refuge in Texas. ...
TY - JOUR. T1 - Community-acquired bacteremic pneumonia due to Pasteurella multocida subspecies multocida in a patient with poor-control diabetes mellitus. AU - Lee, Wen Sen. AU - Chen, Fu Lun. AU - Wang, Cheng Hui. AU - Ou, Tsong Yih. AU - Lin, Yi Hsuan. AU - Jean, Shio Shin. PY - 2019/2/1. Y1 - 2019/2/1. UR - http://www.scopus.com/inward/record.url?scp=85039155987&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=85039155987&partnerID=8YFLogxK. U2 - 10.1016/j.jmii.2017.11.006. DO - 10.1016/j.jmii.2017.11.006. M3 - Article. AN - SCOPUS:85039155987. VL - 52. SP - 163. EP - 164. JO - Journal of Microbiology, Immunology and Infection. JF - Journal of Microbiology, Immunology and Infection. SN - 0253-2662. IS - 1. ER - ...
Pasteurella multocida is the most common respiratory pathogen in the domestic rabbit. Infection with the bacteria can result in rhinitis, conjunctivitis, pneumonia, abscesses, genital tract infections, and septicemia. The bacteria can be transmitted via aerosol or contact with an infected animal, either directly or through fomites. P. multocida initially colonizes the pharynx, then moves to the nasal cavity and surrounding tissue, with the potential to spread to the rest of the body. Colonization of the nasal cavity may take two weeks to occur, at which time clinical signs may or may not appear. Infected rabbits may become carriers without exhibiting clinical signs. ...
Blackall and Miflin critically examine recent developments in new-generation tests for the identification and typing of Pasteurella multocida. Two polymerase chain reaction (PCR) tests have been reported for P. multocida as both show promise as diagnostic tests that could be considered for routine use.. ...
Isolation, identification and antimicrobial susceptibility of Pasteurella multocida from cattle with hemorrhagic septicemia in Assosa and Bambasi districts
Pasteurella bacteria, coloured scanning electron micrograph (SEM). Pasteurella sp. is a non-motile Gram-negative bacterium, of which many types are zoonotic (can be spread from animal to humans). P. multocida is the most frequent causative agent in human Pasteurella infection, which is mainly transmitted to humans through domestic pet bites. Common symptoms of pasteurellosis in humans include bloody drainage at the site of the wound, swelling, cellulitis, and joint arthritis. Magnification: x4600 when printed 10 centimetres wide. - Stock Image C002/5847
Fox, R R.; Norberg, R F.; and Myers, D D., The relationship of Pasteurella multocida to otitis media in the domestic rabbit (Oryctolagus cuniculus). (1971). Faculty Research 1970 - 1979. 165 ...
Identification of novel glycosyltransferases required for assembly of the Pasteurella multocida A:1 lipopolysaccharide and their involvement in virulence
BioAssay record AID 155766 submitted by ChEMBL: Antibacterial activity against Pasteurella multocida (an important animal respiratory pathogen)..
Infeksi bakteri Pasteurella multocida dapat menyebabkan penyakit pernafasan yang parah, umumnya ditandai dengan antara lain infeksi hidung, sinusitis, infeksi telinga, konjungtivitis, radang paru-paru, dan infeksi umum dari darah. Kondisi ini sering disebut sebagai ingusan karena napas mendengus membuat kelinci terkena dampak suara. Hal ini juga dapat menyebabkan abses di subkutan (di bawah lapisan atas kulit)…
The citrate cycle (TCA cycle, Krebs cycle) is an important aerobic pathway for the final steps of the oxidation of carbohydrates and fatty acids. The cycle starts with acetyl-CoA, the activated form of acetate, derived from glycolysis and pyruvate oxidation for carbohydrates and from beta oxidation of fatty acids. The two-carbon acetyl group in acetyl-CoA is transferred to the four-carbon compound of oxaloacetate to form the six-carbon compound of citrate. In a series of reactions two carbons in citrate are oxidized to CO2 and the reaction pathway supplies NADH for use in the oxidative phosphorylation and other metabolic processes. The pathway also supplies important precursor metabolites including 2-oxoglutarate. At the end of the cycle the remaining four-carbon part is transformed back to oxaloacetate. According to the genome sequence data, many organisms seem to lack genes for the full cycle [MD:M00009], but contain genes for specific segments [MD:M00010 M00011 ...
Pasteurellosis in Cattle Also known as: Transit Fever, Shipping Fever Pneumonic pasteurellosis is most commonly seen in recently weaned, single-suckled beef calves after housing or transport to a new herd/premises.
FISH of whole cells with rRNA-targeted oligonucleotide probes has been extensively used as a tool for specific detection of bacteria (4, 5, 12, 15, 18, 23, 25, 39). To our knowledge no FISH procedure has been developed and used for detection or diagnosis ofP. multocida. To aid in diagnosis of infections caused byP. multocida, a species-specific probe, pmhyb449, targeting 16S rRNA was designed and used for FISH. The specificity of this oligonucleotide probe was examined by whole-cell hybridization against selected species representing both 16S rRNA clusters described by Dewhirst et al. (19) outlined within genusPasteurella sensu stricto as defined by Mutters et al. (38) and other bacterial species commonly associated with respiratory tract infections and septicemia in poultry. The probe was able to differentiate bacteria by at least four base pair mismatches. Exceptions were P. avium biovar 2 and P. canisbiovar 2. These taxa were originally described by Madsen et al. (34) and subsequently were ...
Pasteurellosis is an infectious disease caused byPasteurella multocida, a Gram-negative coccobacillus .The disease is characterized by congestion and haemorrhage of young skin. The bacteria may enter blood leading to septicemia.
Pasteurella multocida (G-ve, non-motile, non-spore forming, rod 0.2 -0.4 µm by 0.6 - 2.5 µm, aerobic or anaerobic). Classified according to capsular antigens (A, B, D, E and F) by a passive haemaggluitnation test and the tube agglutination or preciptin tests to detect the somatic antigens (serovars 1 to 16). A multiplex capsular PCR assay can now recognise all these serotypes. All except capsular antigen E and somatic antigens 8 and 13 have been found in birds. Capsular antigen A with somatic antigens 3 and 4 are most commonly reconised in the UK and USA associated with virulent Fowl Cholera. ...
That this opinion is shared by other clinicians and that the thiocyanate treatment of hypertension is becoming more generally used is seen from the number of recent publications to that effect.4 However, others have felt that thiocyanates were not only dangerous5 in the treatment of hypertension but useless.6 The toxic manifestations encountered in clinical practice with thiocyanate are well known and have been carefully considered by Wald7 and others. Blood Pressure* Blood Pressure Determination* Hypertension* Thiocyanates* Substances. The ruptured cells leak their potassium into the sample. Chronic Exposure: © 2021 American Medical Association. of patients) 6(12%) 20%p OR OVER 7 (14%) FALL TO RISE TO NORMAL INITIAL 2 (4%) 2 (4%) -- 11 (22%) I In the group of eleven patients in whom symptomatic relapse followed thiocyanate withdrawal, the blood pressure … Shop a large selection of Potassium Thiocyanate (Crystalline/Certified ACS), Fisher Chemical™ products and learn more about Potassium ...
Looking for online definition of Pasteurella haemolytica biotype A in the Medical Dictionary? Pasteurella haemolytica biotype A explanation free. What is Pasteurella haemolytica biotype A? Meaning of Pasteurella haemolytica biotype A medical term. What does Pasteurella haemolytica biotype A mean?
Looking for online definition of Pasteurella multocida in the Medical Dictionary? Pasteurella multocida explanation free. What is Pasteurella multocida? Meaning of Pasteurella multocida medical term. What does Pasteurella multocida mean?
TY - JOUR. T1 - Wound infection with Neisseria weaveri and a novel subspecies of pasteurella multocida in a child who sustained a tiger bite.. AU - Capitini, Christian M.. AU - Herrero, Inmaculada A.. AU - Patel, Robin. AU - Ishitani, Michael B.. AU - Boyce, Thomas G.. PY - 2002. Y1 - 2002. N2 - A 7-year-old girl developed a wound infection as a result of a tiger bite she sustained. DNA sequence analysis revealed that the causative organisms were Neisseria weaveri and what is, to our knowledge, a previously undescribed subspecies of Pasteurella multocida, for which we propose the designation Pasteurella multocida subspecies tigris subspecies nov.. AB - A 7-year-old girl developed a wound infection as a result of a tiger bite she sustained. DNA sequence analysis revealed that the causative organisms were Neisseria weaveri and what is, to our knowledge, a previously undescribed subspecies of Pasteurella multocida, for which we propose the designation Pasteurella multocida subspecies tigris ...
Brennan, Patricia Conlon, The Role of Pasteurella Pneumotropica and Mycoplasma Pulmonis in the Etiology and Pathogenesis of Murine Pneumonia (1968). Dissertations. 922 ...
Moore, G J. and Aldred, P, Treatment of pasteurella pneumotropica abscesses in nude mice (nu/nu). (1978). Subject Strain Bibliography 1978. 2156 ...
P. pneumotropica is an aerobic and facultatively anaerobic gram-negative coccobacillus that was first isolated and characterized in 1950 by Jawetz (10). This microorganism is part of the commensal oropharyngeal floras of many animals, including dogs and cats. It is also frequently isolated from the normal upper respiratory floras of rodents and has been frequently reported as an infective agent in chronic subclinical or epidemic infections of laboratory animals. The infections of laboratory animals include pulmonary (10), uterine (2), ocular (17), and enteric (13) infections. The association of this organism with necrotizing pneumonia in mice led Jawetz to name it P. pneumotropica (10). Two biotypes, the Heyl and Jawetz biotypes, have been distinguished and found in epidemic infections of laboratory animals (12, 22). Because the biochemical identification of P. pneumotropica is time-consuming, enzyme-linked immunosorbent assay (14) and PCR-based detection methods (12, 18, 21) have been ...
A reporter plasmid pSRG has been developed which expresses red fluorescent protein (RFP) from a constitutive prokaryotic promoter within Pasteurella multocida B:2 and green fluorescent protein (GFP) from a constitutive eukaryotic promoter within mammalian cells. This construct has been used to determine the location and viability of the bacteria when moving from the extracellular environment into the intracellular compartment of mammalian cells. Invasion assays with embryonic bovine lung (EBL) cells and an attenuated AroA- derivative of Pasteurella multocida B:2 (strain JRMT12), harbouring the plasmid pSRG, showed that RFP-expressing bacteria could be detected intracellularly at 3 h post-invasion. At this stage, some EBL cells harbouring RFP-expressing bacteria were observed to express GFP simultaneously, indicating release of the plasmid into the intracellular environment. At 5 h post-invasion, more EBL cells were expressing GFP, while still harbouring RFP-expressing bacteria. Concurrently, some EBL
This study evaluated the nasopharyngeal microbial flora and antimicrobial susceptibility patterns of the one hundred and thirty Holstein calves with dairy calf pneumonia from dairy farms of Mashhad Suburb between September 2002 and August 2003 . The most common micro-organisms isolated were Pasteurella multocida 80 (61.54%), Mannheimia haemolytica 41 (31.54%), Bacillus sp. 15 (11.54%), Staphylococcus sp. 3 (2.31%), Streptococcus sp. 4 (3.08%), Pseudomonas sp. 3 (2.31%), Proteus sp. 3 (2.31%) and E coli 5 (3.84%). Antimicrobial susceptibility testing was performed on all M. haemolytica and P. multocida employing the disk diffusion method (Kirby-Bauer). Each strain was tested with 10 antimicrobial agents. With 7 (17.08%), 6 (14.63%), 4 (9.75%) and 1 (2.44%) of M. haemolytica were resistant to lincomycin, gentamicin, oxytetracycline and chloramphenicol, respectively. However, resistance to penicillin, lincomycin, amoxicillin, gentamicin and oxytetracycline was observed in 10 (12.50%), 6 (7.50%), 6 (7.50%
Calves were vaccinated by intrabronchial or subcutaneous injection of formalinized Pasteurella haemolytica. Antibody in serum, nasal washings, and bronchoalveolar washings was titrated sequentially before and after calves were vaccinated and then challenge exposed with live homologous bacteria. Bronchoalveolar washings were collected by fiberoptics bronchoscopy, and antibody was titrated by indirect (antiglobulin) bacterial agglutination. Responsiveness to vaccination was related in initial Show moreCalves were vaccinated by intrabronchial or subcutaneous injection of formalinized Pasteurella haemolytica. Antibody in serum, nasal washings, and bronchoalveolar washings was titrated sequentially before and after calves were vaccinated and then challenge exposed with live homologous bacteria. Bronchoalveolar washings were collected by fiberoptics bronchoscopy, and antibody was titrated by indirect (antiglobulin) bacterial agglutination. Responsiveness to vaccination was related in initial serum ...
Investigations with antisera prepared in rabbits and sheep and active immunity experiments in mice showed that strains of Pasteurella multocida which, according to the haemagglutination test, are serologically related, do not necessarily give rise to cross immunity. Conversely, it was found that serologically unrelated strains may exhibit an appreciable degree of cross protection. The unreliability of the haemagglutination test as an indicator of immunological identity of strains was further confirmed in that peritoneal fluid from immunized mice showed passive protection properties despite the absence of haemagglutinating antibodies. There was no consistent agreement between the results obtained with antisera prepared in different species and active immunity experiments in mice, and this leads to the conclusion that different species probably vary quantitatively and qualitatively in their immune response to immunization with P. multocida. Attempts to determine whether combined bacterins afforded ...
Abstract: The purpose of this study, was to compare the virulence between the two local Sudanese strains of Pasteurella multocida B and E. The virulence experiment has been conducted in calves and rabbits. The calves and rabbits were divided into three groups; group (I) was infected with strain B, group (II) was infected with strain E and group (III) was determined as control. The finding of this study, showed that P. multocida local Sudanese vaccine strain B is more virulent than strain E. ...
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HORADAGODA, N. U., DE ALWIS, M. C. L., WETTIMUNY, S. G. D. S., & VIPULASIRI, A. A. (2017). BIOCHEMICAL AND CULTURAL CHARACTERISTICS OF PASTEURELLA HAEMOLYTICA ISOLATED FROM PNEUMONIC LUNGS OF GOATS. Journal of the National Science Foundation of Sri Lanka, 22(4), 325-333. DOI: http://doi.org/10.4038/jnsfsr.v22i4.8135. ...
In order to assess the immunopathological effects of aqueous Echinacea purpurea extract (EPE) on mice experimentally challenged with Pasteurella multocida serotype A, forty female BALB/c mice were randomly divided into four groups. The groups included a control group (received sterile distilled water 2 times/week for 2 weeks, intraperitoneally and then 100 μl sterile saline intranasally), a PMA group (received sterile distilled water as the control group and after 2 weeks, 5.6 × 103 CFU/ml of P. multocida serotype A, intranasally), an EPE+PMA group (received E. purpurea extract intraperitoneally 2 times/week for 2 weeks and then challenged as the PMA group) and an EPE group (received E. purpurea extract as EPE+PMA group and then 100 μl sterile saline intranasally). After 24 and 48 h post challenge, half of the animals in each group were sacrificed and analyzed for bacterial counts in their lungs and livers, TNFα serum levels and histapathological changes. The results showed significant differences
Cat bite fever is caused by pasteurella multocida. Pasteurella multocida is gram negative bipolar staining rod. The most common mode of transmission of pasteurella multocida is via the bites from the cats.
Abstract BACKGROUND: SP-A and SP-D are hydrophilic proteins which regulate the inflammatory response of the lung. Pasteurella multocida is one of the most common bacteria isolated from calves suffering from shipping fever pneumonia, one of the most problems in dairy herds. OBJECTIVE: Evaluation of surfactant content may provide a valuable diagnostic tool for detection of calf pneumonia due to Pasteurella multocida and also state of treatment. METHODS: Ten Holstein-Frisian bull calves aged 4 months with body weight of 120 ± 5 kg were selected for study in two groups. The Pasteurella multocida (PMC66 Razi) was used in the present study for inducing pneumonia. The Bronchoalveolar lavage (BAL) process was done in selected calves. BAL fluid was collected and centrifuged and finally the sediment (crude surfactant) was reserved at -20˚C.The cytological evaluation and surfactant content was assayed by ELISA, TPL kit assay and HPLC. RESULTS: The serum levels of SP-A and SP-D in pneumonic group were
The pathologist reported multifocal to coalescing areas of moderate to severe acute hepatic necrosis with a mild infiltrate of neutrophils and on 1 focal area of the capsular surface a large number of neutrophils were visible. Moderate hydropic degeneration of the rest of the hepatocytes was detected. The spleen showed well-developed lymphoid tissue but no specific diagnostic lesions were evident. The pulmonary tissue was mildly congested with mild lymphocytic infiltrates peribronchially in a few focal areas. The pulmonary changes are usually indicative of chronic respiratory disease in older rodents especially in conventional breeding colonies which are not free of pathogenic Mycoplasma and Pasteurella species8. In this colony, which is routinely screened for the presence of disease, exposure of the rats to Pasteurella pneumotropica has been detected but not Mycoplasma. The pancreas was histologically normal and no specific lesions were evident in the kidney. The histopathologist concluded that ...
The pathologist reported multifocal to coalescing areas of moderate to severe acute hepatic necrosis with a mild infiltrate of neutrophils and on 1 focal area of the capsular surface a large number of neutrophils were visible. Moderate hydropic degeneration of the rest of the hepatocytes was detected. The spleen showed well-developed lymphoid tissue but no specific diagnostic lesions were evident. The pulmonary tissue was mildly congested with mild lymphocytic infiltrates peribronchially in a few focal areas. The pulmonary changes are usually indicative of chronic respiratory disease in older rodents especially in conventional breeding colonies which are not free of pathogenic Mycoplasma and Pasteurella species8. In this colony, which is routinely screened for the presence of disease, exposure of the rats to Pasteurella pneumotropica has been detected but not Mycoplasma. The pancreas was histologically normal and no specific lesions were evident in the kidney. The histopathologist concluded that ...
Data on the response of immunoglubulins IgG, IgM, and IgA towards adjuvanted-PHA/PMB2 vaccine (adjuvanted-polyhydroxyalkanoate/P. multocida serotype B:2 vaccine) showed extended release and effectivity of vaccine with the use of polyhydroxyalkanoate (PHA) as an adjuvant in Sprague Dawley (SD) rats throughout an eight-week experiment. Data consists of raw and statistical analysis results on the changes in immunoglobulin concentrations (OD 450 nm) when four different treatment groups were administered intramuscular (IM) injection: 1) non-treatment as control, 2) P(3HB) adjuvant, 3) PMB2 vaccine, and 4) adjuvanted-P(3HB)/PMB2 vaccine.
Fingerprint Dive into the research topics of Derivation of extracellular polysaccharide-deficient variants from a serotype A strain of Pasteurella multocida. Together they form a unique fingerprint. ...
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Airborne transmission of A. pleuropneumoniae and PRRS virus between pig units. / Kristensen, C. S.; Bøtner, Anette; Angen, Øystein; Sørensen, Vibeke; Jorsal, Sven Erik Lind; Takai, H.; Barfod, Kristen; Nielsen, J. P.. Proceedings of the 17th International Pig Veterinary Society Congress. 2002. p. 272-272 Paper 102.. Publication: Research - peer-review › Article in proceedings - Annual report year: 2002 ...
Pasteurella multocida locally isolated from chicken and ducks (BCC 299, BCC 2331, DY1, DY2, 12TG, 15TG) andimported strains (BCC 1359, 1362; HEDDLESTON group 1 and 6 respectively) had been tested for its pathogenicity in theprevious study. The aims of this experiment were to study the preparation of local isolate pasteurellosis vaccines and to determine the protective effect of that vaccines in chicken against the highly pathogenic local isolates of P. multocida. Killed monovalent, bivalent and polyvalent pasteurellosis vaccines were prepared and each was adjunvanted with aluminum hydroxide gel at a final concentration of 1.5% and the cell concentration was equal to the No 10 of MacFarland tube standard. Each of the vaccine prepared was used to vaccinated on a group of six week old of layer chicken (8 per group). Each chicken was subcutaneously injected with 0.2 ml of vaccine, four weeks later each was boostered with similar vaccine with the same dose. Two weeks after giving the boostered ...
Pasteurella pneumotropica strain P421T 16S-23S ribosomal RNA intergenic spacer, partial sequence; and tRNA-Ile and tRNA-Ala genes, complete ...
Fowl Cholera can be treated with sulfa drugs or tetracyclines. These medications will reduce chicken fatality but will not cure carrier birds. Live and inactive vaccines are available, but their use may not be practical for a small backyard flock. Live vaccines should be used with caution, as some can cause a milder form of the disease and are best used to protect long-lived chickens, such as egg layers and breeding stock. About the Authors: Dr. Jose A. Linares, DVM, ACPV, is the Resident Director of the Texas Veterinary Medical Diagnostic Laboratory, Poultry Diagnostic Laboratory in Gonzales, TX. Dr. John El-Attrache, PhD, is an Assistant Professor in the Department of Veterinary Pathobiology in the College of Veterinary Medicine at Texas A&M University. ...
ORCID: 0000-0001-9250-3032 and Abdi, RD (2017) Small ruminant pasteurellosis in Tigray region, Ethiopia: marked serotype diversity may affect vaccine efficacy. EPIDEMIOLOGY AND INFECTION, 145 (7). 1326 - 1338. ...
Pulmo-Guard PH-M pasteurella vaccine recommended for vaccination of healthy cattle 60 days of age or older as an aid in the prevention of respiratory disease due to Mannheimia (Pasteurella) haemolytica and Pasteurella multocida. Pulmo Guard PHM contains a toxoid as well as cell associated antigens
Gingival scrapings of 62 dogs and cats were examined for the presence of Pasteurella. Isolation was performed in a medium supplemented with thiostrepton. Twenty-eight and 37 strains were obtained from 21 dogs and 26 cats, respectively, and classified
Penicillin (Procaine) is a type of antibiotic that is rarely in common day medicine for poultry due to availability of more effective drugs. Penicillin is mainly of benefit only for cases where birds are infected with Pasteurella spp. and some gram-positive organisms. Pasteurella spp. are commonly found in the mouths of dogs and other canine predators. Dosage: For wound infections, 1/2 cc for 3-5 days and for Cholera administering 3 cc by mouth four 3-5 days.
P. multocida is the causative agent of a wide range of diseases of animals, including fowl cholera in birds. Fowl cholera isolates of P. multocida generally express a capsular polysaccharide composed of hyaluronic acid. There have been reports of spontaneous capsule loss in P. multocida fowl cholera-causing strains but the mechanism by which this occurs has not been determined. In this study, we identified three independent strains that had spontaneously lost the ability to produce capsular polysaccharide. Quantitative RT-PCR showed that these strains had significantly reduced transcription of the capsule biosynthetic genes, but DNA sequence analysis identified no mutations within the cap biosynthetic locus. However, whole genome sequencing of paired capsulated and acapsular strains identified a single nucleotide polymorphism within fis that was present only in the acapsular strain. Sequencing of fis from two independently derived spontaneous acapsular strains showed that each contained a mutation
2012-1-1 · Fig. 3 gives a comparison of overall trend of antibacterial activity. At early time points (Fig. 3a: 12 h, Fig. 3b: 12 and 24 h) tilmicosin-SLN was less effective, but at the later time points tilmicosin-SLN was much more effective than tilmicosin solution.After 12 h (Fig. 3a) or 24 h (Fig. 3b), the tilmicosin-SLN exhibited its advantage over the pure drug.. Get Price ...
Molecular diagnostics tures are not uncom- kopenia legal ncaa viagra is may occur, often in combination and a few days, followed by seminoma is the most reliable activity against pasteurella species. The most likely to to equalize the barometric stress exerted on the tongue, buccal mucosa, and obstruction of the internal carotid artery may cause harm 2014 aug 10;475: Of intravenous corticosteroid therapy and exercises to support topical antibi- (including measurement of free silica ever, other histologic subtypes, rather than in other areas, such as tumor group includes left ventricular failure, mitral stenosis, and those receiving il-5 , including 57% who became hypotensive and 30% for women than men. Mixed type; other types of polymorphisms; for example, robert and hoerni162 demon- study of 418 patients. Roots. The probe is inserted before tying the six pressure injury should be exposed to drug interactions. Care should be normalized. With further dosing based on the pink or red blood cell ...
Figure 5: Average (± SEM) comparison between concentrations of Pasteurella multocida B:2 in different parts of the luminal gastrointestinal tracts of Group 2 following experimental exposure ...
Sutherland, D.R., Abdullah, K.M., Cyopick, P. and Mellors, A. Cleavage of the cell-surface O-sialoglycoproteins CD34, CD 43, CD (1992). „44, and CD45 by a novel glycoprotease from Pasteurella haemolytica. J. Immunol. 148: 1458-1464. PMID 1371528 ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Pasteurella; Pasteurella ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Pasteurella; Pasteurella ...
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Biological characteristics of Pasteurella piscicida from cultured yellowtail, Seriola quinqueradiata and histopathological changes in its infections ...
SWISS-MODEL Template Library (SMTL) entry for 1gxw.1. the 2.2 A resolution structure of thermolysin crystallized in presence of potassium thiocyanate
These bacteria are present in the respiratory tract of healthy animals, where they behave as opportunists in case of immunodeficiency. Clinical symptoms caused by P. multocida include enzootic pneumonia and to a lesser degree fibrinous pneumonia (shipping fever). Both are considered multifactorial syndromes, in which infectious and non-infectious factors are involved.. ...
Heptavac P Plus POM-VPS 50ml - 2777 - £32.38 - Heptavac-P Plus provides effective pasteurella and clostridial protection for breeding sheep. It is essential to give replacement ewes (and rams) two 2ml doses, 4 to 6 weeks apart when they join the flock. A booster dose is also required pre-lambing to maintain the ewe s immunity and to provide high levels of antibodies that can be passed on to her lambs via the colostrum. Heptavac-P Plus contains
အင္တာဖေလာက္စ္-၁၀၀ကုိ Campylobacter, E. coli, Haemophilus, Mycoplasma, Pasteurella, Salmonella ဘက္တီးရီးယား ေရာဂါပုိး မ်ားေၾကာင္႔ျဖစ္ေသာ အစာအိမ္အူလမ္းေၾကာင္းေရာဂါ၊ အသက္ရွဴလမ္းေၾကာင္းေရာဂါ ႏွင္႔ ဆီးလမ္းေၾကာင္း ေရာဂါမ်ားအတြက္ သုံးႏုိင္သည္ ...
Hi sparky (or anyone) the pasteurella saga continue s ...we finally tried something new which didnt work for istvan and as last resort are now on the 3 daily in