TY - JOUR. T1 - Paramyxovirus V protein interaction with the antiviral sensor LGP2 disrupts MDA5 signaling enhancement but is not relevant to LGP2- mediated RLR signaling inhibition. AU - Rodriguez, Kenny R.. AU - Horvath, Curt M.. PY - 2014/7. Y1 - 2014/7. N2 - The interferon antiviral system is a primary barrier to virus replication triggered upon recognition of nonself RNAs by the cytoplasmic sensors encoded by retinoic acid-inducible gene I (RIG-I), melanoma differentiation-associated gene 5 (MDA5), and laboratory of genetics and physiology gene 2 (LGP2). Paramyxovirus V proteins are interferon antagonists that can selectively interact with MDA5 and LGP2 through contact with a discrete helicase domain region. Interaction with MDA5, an activator of antiviral signaling, disrupts interferon gene expression and antiviral responses. LGP2 has more diverse reported roles as both a coactivator of MDA5 and a negative regulator of both RIG-I and MDA5. This functional dichotomy, along with the ...

Genetic data from avian influenza and avian paramyxoviruses generated by the European network of excellence (EPIZONE) between 2006 and 2011-Review and recommendations for surveillance. Dundon William G., Heidari Alireza, Fusaro Alice, Monne Isabella, Beato Maria Serena, Cattoli Giovanni, Koch Guus, Starick Elke, Brown Ian, Aldous Elisabeth W., Briand François-Xavier, Le Gall-Reculé Ghislaine, Jestin Véronique, Jorgensen Poul H., Berg Mikael, Zohari Siamak, Metreveli Giorgi, Munir Muhammad, Stahl Karl, Albina Emmanuel, Hammoumi Saliha, Gil Patricia, Servan de Almeida Renata, Smietanka Krzysztof, Domanska-Blicharz Katarzyna, Minta Zenon, Van Borm Steven, Van den Berg Thierry, Moreno Martin Ana, Barbieri Ilaria, Capua Ilaria. 2012. Veterinary Microbiology ...

A comprehensive survey of current basic research on the paramyxo-viruses. Specialists actively working in the discipline present a variety of interpretations of paramyxovirus taxonomy, evolution, genetics, molecular biology, and epidemiology; interactions between paramyxoviruses and host cells; acut

This review evaluates possible mechanisms which regulate paramyxovirus protein and RNA syntheses. Evidence indicates that viral polypeptide abundances are specified at the transcriptional level. RNA...

Family-wide molecular diagnostic assays are valuable tools for initial identification of viruses during outbreaks and to limit costs of surveillance studies. Recent discoveries of paramyxoviruses have called for such assay that is able to detect all known and unknown paramyxoviruses in one round of PCR amplification. We have developed a RT-PCR assay consisting of a single degenerate primer set, able to detect all members of the Paramyxoviridae family including all virus genera within the subfamilies Paramyxovirinae and Pneumovirinae. Primers anneal to domain III of the polymerase gene, with the 3′ end of the reverse primer annealing to the conserved motif GDNQ, which is proposed to be the active site for nucleotide polymerization. The assay was fully optimized and was shown to indeed detect all available paramyxoviruses tested. Clinical specimens from hospitalized patients that tested positive for known paramyxoviruses in conventional assays were also detected with the novel family-wide test. A high

Looking for online definition of paramyxoviruses in the Medical Dictionary? paramyxoviruses explanation free. What is paramyxoviruses? Meaning of paramyxoviruses medical term. What does paramyxoviruses mean?

Summary Chickens were immunized with eight different serotypes of avian paramyxovirus (PMV). Ninety percent of splenic IgG antibody-forming cells (AFC) were serotype-specific with respect to the glycoprotein and nucleoprotein-polymerase antigens in an indirect immunoperoxidase binding system. The IgG AFCs which cross-reacted between serotypes divided the serotypes into two mutually exclusive super serogroups composed of PMV-1, -3, -4, -7 and -9, and PMV-2, -6 and -8. PMV-5 was not tested.

TY - CHAP. T1 - Experimental Characterization of Fuzzy Protein Assemblies. T2 - Interactions of Paramyxoviral NTAIL Domains With Their Functional Partners. AU - Troilo, Francesca. AU - Bignon, Christophe. AU - Gianni, Stefano. AU - Fuxreiter, Monika. AU - Longhi, Sonia. PY - 2018. Y1 - 2018. N2 - In this chapter we detail various experimental approaches to characterize the fuzziness of complexes made of the C-terminal domain of the nucleoprotein (NTAIL) from three representative paramyxoviruses and of the C-terminal X domain (XD) of the homologous phosphoprotein. We discuss the advantages, the limitations, as well as the caveats of the various methods. We describe experimental data showing that paramyxoviral NTAIL-XD complexes are characterized by a considerable amount of conformational heterogeneity. We also detail recent data that revealed that NTAIL is highly malleable, i.e., it displays a partner-mediated polymorphism. All the results suggest that NTAIL plasticity and fuzziness play a role ...

Characterization of the J virus or, in keeping with recent nomenclature recommendations, J paramyxovirus (JPV) genome revealed a unique genome structure, consisting of eight genes in the order 3′-N-P/V/C-M-F-SH-TM-G-L-5′. The small hydrophobic (SH) protein and the transmembrane (TM) protein genes are predicted to encode proteins 69 and 258 aa in size, respectively. The 4401 nt attachment (G) protein gene, much larger than most other paramyxovirus attachment protein genes sequenced to date, encodes a putative 709 aa attachment protein and contains distally a second open reading frame (ORF-X) 2115 nt long. Experiments undertaken in this study were intended to confirm the sequence-based gene allocation of JPV and to determine if proteins encoded by the SH gene, the novel TM gene and ORF-X are expressed. Northern blot analyses carried out on mRNA purified from JPV-infected cells indicated that the putative transcription initiation and termination sequences flanking the SH and TM genes are functional,

Structure of the Rb C-terminal domain bound to E2F1-DP1: A mechanism for phosphorylation-induced E2F release. Structure of DDB1 in complex with a paramyxovirus v protein: Viral hijack of a propeller cluster in ubiquitin ligase

La diagnose clinique ortho-, un chef global de la diagnose in vitro avec une histoire riche de porter les tests critiques pour que les maladies infectieuses lancent sur le marché, aujourdhui

CONSULTANT is supported by a database consisting of approximately 500 signs/symptoms, about 7,000 diagnoses/causes, and about 18,000 literature references including over 3000 web references. Presenting the main concepts, this book leads students as well as advanced researchers from different disciplines to an understanding of current ideas in the complex field of comprehensive experimental investigation of biological objects, analysis of data, development of models, simulation, and hypothesis generation Veterinary Immunology Principles and Practice by Day, Michael J. [Thieme Medical Pub,2010] (Paperback) Veterinary Immunology Principles and Practice by Day, Michael J. [Thieme Medical Pub,2010] (Paperback) online. You can see 5 to 6 samples of other lectures youll be studying with each of these classes if you follow the links at the bottom of the TU classs web page. Animal Behavior studies the evolution of behavior in animals and brain evolution. Topics for discussion include aggressive ...

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INTRODUCTION Pigeons are birds of European origin found worldwide. Due to their high population growth, they act as important hosts in the spread of diseases in humans and other animal species, and transmission occurs via feces (Haag & Gurdan, 1990; Orlandella et al., 1992, Toro et al. 1999). In nature, they play an important role as insect control and replanting of seeds eliminated by feces to the soil (Museu de Ciências Naturais).. In these species, the most important viral diseases of the digestive tract are transmitted by the paramyxovirus or adenovirus (Marlier & Vindevogel, 2006) followed by coronavirus (Qian et al., 2006).. Avian paramyxoviruses are non-segmented single stranded RNA negative polarity, enveloped with helical symmetry of the nucleocapsid. Virions are pleomorphic, usually roughly spherical, 150 nm or more in diameter, but filamentous forms are common (Alexander et al., 1993).. They belong to the Mononegavirales order, Paramyxoviridae family, Paramixovirinae sub-family and ...

Conflicting reports exist regarding the requirement for virus replication in interferon (IFN) induction by paramyxoviruses. Our previous work has demonstrated that pathogen-associated molecular patterns capable of activating the IFN-induction cascade are not normally generated during virus replication, but are associated instead with the presence of defective interfering (DI) viruses. We demonstrate here that DIs of paramyxoviruses, including parainfluenza virus 5, mumps virus and Sendai virus, can activate the IFN-induction cascade and the IFN-β promoter in the absence of virus protein synthesis. As virus protein synthesis is an absolute requirement for paramyxovirus genome replication, our results indicate that these DI viruses do not require replication to activate the IFN-induction cascade.

In the last few years there has been a growing interest towards intrinsically disordered proteins related to the understanding of the numerous and diverse functional roles they play. Our group played a pioneering role in focusing the attention of virologists on the abundance of disorder within proteins of the replicative complex of paramyxoviruses. Following these seminal reports in the early 2000s, many other studies that addressed the disordered nature of various viral proteins have been published in subsequent years, leading to revisiting the classical way structure-function relationships in viral proteins are seen. The main advantage of disorder within viral proteins has been proposed to be related to (i) mutational robustness, (ii) pleiotropy, (iii) genetic compaction and (iv) the lessening of evolutionary constraints on overlapping reading frames. In the coming years we aim at further extending these studies by assessing the disordered state of disordered regions from paramyxoviral ...

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Vipinkumar, V P and Harshan, N K and Sunil, P V and Jeethumol, T J and Athira, P V and Narayanakumar, R and Gopalakrishnan, A (2017) വിജയസാഫല്യം അക്വാടൂറിസത്തിലൂടെ: മാതൃകയായി വൈപ്പിനിലെ വനിതാ സ്വാശ്രയസംഘങ്ങൾ. [Video] Vipinkumar, V P and Vidya, R and Harshan, N K and Sunil, P V and Jeethumol, T J and Athira, P V and Narayanakumar, R and Gopalakrishnan, A (2017) ഫെർട്ടിഫിഷ്: എങ്ങണ്ടിയൂരിലെ വനിതാ സംരംഭകർക്ക്‌ ഉപജീവനം വൈവിദ്ധ്യവത്ക്കരണത്തിലൂടെ. [Video] Vipinkumar, V P and Mohamed, K S and Nair, Rekha J and Ramachandran, C and George, Grinson and Ignatius, Boby and Jayasankar, J and Gopalakrishnan, A (2017) 7 Decades of CMFRI - A Reminiscence. [Video] Loka, Jayasree and Philipose, K K and Sonali, S M and Santhosh, B and ...

A comprehensive study using virological and serological approaches was carried out to determine the occurrence of avian paramyxoviruses (APMVs) and avian influenza viruses (AIVs) in live healthy mallard ducks (Anas platyrhynchos) in addition to caged birds, wild birds (other than waterfowl), and poultry. Thirty-three viruses were isolated from 321 tracheal and cloacal swabs from mallard ducks and were characterised as: 6 AIV (two H5N2 and four H4N6), 10 APMV-1, and 17 APMV-4. Of 335 serum samples tested for AIV antibodies, 109 (32.5%) sera were positive by nucleoprotein-blocking ELISA (NP-B-ELISA). Serum samples (315) were examined for antibody to APMV-1, -2, -3, -4, -6, -7, -8, and -9 by the haemagglutination inhibition (HI) test. The largest number of reactions, with titres up to ≥1/64, was to APMV-1 (93.1%), followed by APMV-6 (85.1%), APMV-8 (56%), APMV-4 (51.7%), APMV-7 (47%), APMV-9 (15.9%), APMV-2 (13.3%), and APMV-3 (6.0%). All of the H5N2 isolates of AIV and the APMV-1 isolates from ...

Pigeon paramyxovirus type 1 (PPMV-1) is an antigenic variant of avian paramyxovirus type 1, which mainly infects pigeons. Here, we characterized ten PPMV-1 viruses isolated from pigeons in China during 1996-2019. Phylogenetic analysis of available complete genomes, F and HN genes of PPMV-1 from China showed that multiple PPMV-1 genotypes (I, II, VI, and VII) exist in pigeons in China. Ten PPMV-1 viruses isolated in this study belonged to genotypes VI.1.2.2.2, VI.2.1.1.2.1, VI.2.1.1.2.2 and VII respectively. Genotype VI is predominant in pigeons. VI.2.1.1.2.2 contains most recently isolated PPMV-1 viruses, suggesting that VI.2.1.1.2.2 is a prevalent genotype in pigeons in China. In vitro and in vivo studies showed that four representative viruses from genotypes VI.2.1.1.2.1 (TA14), VI.2.1.1.2.2 (SD19), VI.1.2.2.2 (SD16), and VII (JN08) could replicate efficiently in chicken embryo fibroblasts, while the replication titer of JN08 (VII) virus was significantly lower than that of VI gene viruses in ...

Awang, I.P.R. (1989) The influence of breed of the chick embryo and the route of egg inoculation on the infectivity of turkey paramyxovirus type 3. In: Malaysian Microbiology Symposium, 22-23 Aug 1989, Kuala Lumpur, Malaysia. pp. 36-37. ...

Definition : Serology reagents intended to detect immunoglobulin M (IgM) antibodies to Parainfluenza type 1 virus, a single-stranded RNA virus of the genus Paramyxovirus, family Paramyxoviridae. This virus is an etiologic agent of parainfluenza and several upper respiratory tract infections (e.g., bronchitis, pneumonia) of varying severity.. Entry Terms : Reagents, Serology, Virus, Parainfluenza 1, IgM Antibody. UMDC code : 22419 ...

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Protein-mediated membrane fusion is essential for maintaining eukaryotic cell organization and propagation of major human viruses. Many clinically relevant memb...

This project is concerned with how the recently discovered paramyxovirus, human metapneumovirus (HMPV), interacts with host cells to initiate entry. In this dissertation I explore whether the HMPV fusion protein interacts with cellular proteins during virus attachment and whether receptor binding is sufficient to initiate virus-mediated fusion at the cell surface. In this research I developed novel techniques to study HMPV fusion and entry. I learned that the HMPV fusion protein uses RGD-binding integrins to mediate attachment and virus entry, binding to integrins is not sufficient to trigger HMPV fusion, and HMPV fuses with intracellular membranes instead of at the cell surface. In sum, I have elucidated mechanisms that HMPV utilizes to mediate virus entry, which are unique from the entry mechanism used by other paramyxoviruses ...

This group of viruses can present in many different ways in avian species. The most known about and most publicized paramyxovirus is the one that causes PDD. This disease is more correctly named neuropathic ganglioneuritis. This paramyxovirus affects the nerve ganglia of the gastrointestinal tract, most often causing proventricular and ventricular dilation. A crop biopsy, serum sample and fecal sample are the test samples of choice for a veterinarian to submit to the University of Georgia for screening of this virus. A negative test means nothing, a positive test means your bird is infected.. Other Paramyxoviridae appear to be the most common cause of the encephalomyelitis infections. These viruses affect the ganglia and blood vessel walls in the brain and spinal cord. Basically, a bleed occurs in the brain or spinal cord, and the blood damages adjacent tissues and destroys the proper nerve transmission capabilities of the surrounding tissues. This is similar to a stroke in a human. The location ...

For determination of Reactive (Ortho-) Phosphorus (Phosphate) by the Amino Acid Method. Hach Method 8178. Range: 0.23 - 30.00 mg/L PO4. Sample Size: 25 mL Set includes: Amino Acid Reagent and Molybdate Reagent for 100 tests.

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The Newcastle disease virus (NDV) infects domestic and wild birds, having been described in at least 241 species. NDV is an enveloped, single stranded RNA virus within the family of Paramyxoviridae, genus Avulavirus. There are ten serotypes of avian paramyxoviruses named APMV-1 to APMV-10; NDV is known as APMV-1. Depending upon the disease signs and lesions in chicken, NDV can

For many years, ND has been one of the most important infectious pigeon diseases in China. In recent years, a high mortality has been observed in ND-infected pigeons in China. Mortality is from 40% to 80% or 100% in some cases. The full-length genomes of four pigeon paramyxovirus type 1 (PPMV-1) strains, which were isolated from infected pigeons in China in 2012 and 2013, were sequenced and analyzed to determine the phylogenetic characteristics of PPMV-1 circulating in pigeons of China in recent years. Furthermore, cross hemagglutination inhibition and cross virus neutralization assays, as well as animal experiments were conducted to determine the antigenicity and pathogenicity of those viruses. Proteolytic cleavage sites (residues 112-117) of the F proteins were identified as the typical virulence motif, (112)RRQKR↓F(117) for all four PPMV-1 strains investigated. Phylogenetic analysis based on sequences of complete genomes and F gene revealed that the four PPMV-1 isolates and most of recent ...

Newcastle disease virus Sinonim : avian paramyxovirus type I, pneumoencephalitis virus & pseudo-fowl pest Penyakit viral menular dan fatal yang menyerang beberapa spesies unggas, ayam, kalkun, merpati, itik, angsa, puyuh, dan parkit.

Definition : Serology reagents intended to detect immunoglobulin G (IgG) antibodies to Parainfluenza type 3 virus, a single-stranded RNA virus of the genus Paramyxovirus, family Paramyxoviridae. This virus is an etiologic agent of parainfluenza and several upper respiratory tract infections (e.g., bronchitis, pneumonia) of varying severity.. Entry Terms : Reagents, Serology, Virus, Parainfluenza 3, IgG Antibody. UMDC code : 22424 ...

virus (MV) a member from the paramyxovirus family members remains a primary reason behind worldwide morbidity and mortality getting in charge of approximately 300 0 to 400 0 fatalities annually regardless of the existence of the live-attenuated vaccine (8 50 Globally measles may be the leading reason behind childhood loss of life from a vaccine-preventable disease (http://www. later sequela occurring years following the principal an infection (19 22 Presently no therapeutics are for sale to case administration of serious measles or the fast silencing of regional outbreaks. Ribavirin the only real medication available for the treating some paramyxovirus attacks (9 41 continues to be utilized experimentally for the treating measles but with limited effectiveness (2). This makes appealing the introduction of cost-effective antivirals against MV that augment the prevailing vaccination system. MV infection is set up by pH-independent fusion from the viral envelope with the prospective cell plasma ...

It is an acute, extremely contagious, viral infection that occurs epidemically. Being a seasonal disease, it frequently manifests during late Winter or early Spring. Caused by a virus belonging to the Paramyxoviridae family, it is characterized by fever, enanthema and exanthema. It is spread by airborne transmission through small droplets of pflugge (small respiratory secretions exhaled when an infected person talks, coughs or sneezes). The natural reservoir for the virus are human beings. The disease is generally contracted during childhood although with the current vaccination calendar this has become infrequent. Therefore, all those who have not been vaccinated or who have not contracted the disease are prone to contract it ...

Watch the video lecture Measles Virus - Paramyxoviruses & boost your knowledge! Study for your classes, USMLE, MCAT or MBBS. Learn online with high-yield video lectures by world-class professors & earn perfect scores. Save time & study efficiently. ➨ Try now for free!

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Også kjent som mukolipidose type I Det finnes fire ulike typer mukolipidose: Sialidose (Mukolipidose type I, ML I) Mukolipidose type II (ML II, omtalt et annet sted på våre nettsider) Mukolipidose type III (ML III, omtalt et annet sted på våre nettsider)…. ...

Four groups of signs were identified in the MCA: general ocular signs, serious ocular lesions, respiratory disease, and cutaneous lesions. A virus of the Paramyxoviridae family, genus Henipavirus that infects horses and humans and experimentally guinea pigs and cats. a highly contagious disease of turkeys caused by an enterovirus. A virus of the Paramyxoviridae family, genus Henipavirus that infects horses and humans and experimentally guinea pigs and cats. Diagnosis is made via rapid blood culture or other blood test. The treatment of infection with human herpesvirus 2 is by topical or oral anti-viral medication. A virus of the Paramyxoviridae family, genus Henipavirus that infects horses and humans and experimentally guinea pigs and cats.. On the downside, kissing a child on or around the mouth can transmit your oral bacteria to your child, which can lead to cavities for her down the road see page 100, not to mention the type 1 herpesvirus, or cold sore. The treatment of infection with human ...

Canine distemper infection is caused an individual from the sort Morbillivirus in the family Paramyxoviridae. Canine distemper has been recorded in household pooches for ..

We examined the sites of phosphorylation and the role that phosphorylation plays in the function of the M2-1 protein in transcription and interaction with viral RNA. The M2-1 protein was found by proteolytic digestion and site-directed mutagenesis to be phosphorylated at serine 58 and serine 61. Serines 58 and 61 lie in the consensus sequence for phosphorylation by CKI and are conserved in human, bovine, and ovine RS virus and in turkey rhinotracheitis virus (1, 8, 26, 29, 35, 37). The conservation of these residues suggests a functional role for M2-1 phosphorylation. Phosphorylation of serine 58 creates a new CKI site at serine 61. The disruption of serine 58 should prevent phosphorylation at serine 61, which would result in the loss of both phosphorylation sites, which is consistent with our results. Based on sequence analysis, mutation at serine 61 would be expected to affect phosphorylation at only that site and not at serine 58, explaining why the S61A mutant retains 33P incorporation. The ...

Morbillivirus Infections: Infections with viruses of the genus MORBILLIVIRUS, family PARAMYXOVIRIDAE. Infections mainly cause acute disease in their hosts, although in some cases infection is persistent and leads to degenerative conditions.

INTRODUCTION: Sendai virus (SeV), also known as murine parainfluenza virus type 1 or hemagglutinating virus of Japan (HVJ), is a nonsegmented negative sense single-stranded RNA virus of the Respirovirus genus of the family Paramyxoviridae. SeV is one of the most important respiratory pathogens of rats and mice. SeV is responsible for a highly transmissible respiratory tract infection in mice, hamsters, guinea pigs, rats, and occasionally pigs, with infection passing through both air and direct contact routes. Clinical symptoms seen in mice are signs of pneumonia, such as dyspnea, chattering teeth, and death in young mice. Multiple strains of SeV have been described. The SeV Z strain has a 15,384-nucleotide RNA genome, consisting of six genes. Each gene has concise transcription initiation and termination signals and is transcribed to mRNA encoding a single polypeptide (except for the P gene encoding P, C and V proteins). SeV particles are spherical in shape with an average diameter of 260 nm. A ...

We have found that the entrapment of neuraminidase-treated lymphocytes in the liver leads to the induction of autoimmune cellular cytotoxic reactions.. Lymphocytes from mouse spleen and thymus were incubated with neuraminidase in vitro and injected i.v. into syngeneic recipients. Lymphocytic infiltrations into the liver were seen 7 days later with both types of cells. After repeated weekly injections of asialo-lymphocytes, destruction of liver tissue became apparent. Electronmicroscopic studies showed that hepatocytes, fat storage cells, and endothelial cells were affected, mainly at the hepatic periphery.. It is concluded that the adhesion of asialo-lymphocytes to liver cells induces their cytotoxic activity. Similar reactions may occur after paramyxovirus infection due to the action of viral neuraminidase.. ...

RSV (Respiratory syncytial virus) is a virus that causes respiratory tract infections. RSV is a negative-sense, single-stranded RNA virus of the family Paramyxoviridae, which includes common respiratory viruses such as those causingmeasles and mumps. RSV is a member of the paramyxovirus subfamily Pneumovirinae. RSV is a major cause of lower respiratory tract infections and hospital visits during infancy and childhood.. ...

RSV (Respiratory syncytial virus) is a virus that causes respiratory tract infections. RSV is a negative-sense, single-stranded RNA virus of the family Paramyxoviridae, which includes common respiratory viruses such as those causingmeasles and mumps. RSV is a member of the paramyxovirus subfamily Pneumovirinae. RSV is a major cause of lower respiratory tract infections and hospital visits during infancy and childhood.. ...

Synonyms for Rhabdoviridae in Free Thesaurus. Antonyms for Rhabdoviridae. 6 words related to Rhabdoviridae: arborvirus, arbovirus, vesiculovirus, rhabdovirus, lyssavirus, family. What are synonyms for Rhabdoviridae?

The fragment of benzene comprises to the structure of more than 500 FDA-approved drugs. In 2012, Stepan and coworkers showed that bicyclo[1.1.1]pentane skeleton could act as a saturated nonclassical phenyl ring bioisostere. Adding one carbon atom gives the closest homologue - bicyclo[2.1.1]hexane. The lack of the practical synthetic approaches restricts the common use of bicyclo[2.1.1]hexanes in chemistry. Herein we have designed and synthesized a library of saturated mimics of the ortho- and meta-benzene ring for drug design.. ...

Penyakit Akibat Virus pada Anak Mumps (Gondongan) Measles (Campak) Rubella (Campak Jerman) Measles (Campak) Measles, suatu virus dari genus Morbilivirus dari family Paramyxoviridae. Merupakan virus RNA untai negative, hanya ada 1 serotipe, berenvelope Virus ini mengkode 6 protein structural, termasuk 2 glikoprotein transmembran, fusi (F), dan hemaglutinin (H), yang memfasilitasi masuknya virus dan perlekatan ke…

Resumo: O metapneumovírus aviário (AMPV) é o agente primário da rinotraqueíte dos perus (TRT). O AMPV pertence à família Paramyxoviridae, subfamília Pneumovirinae, gênero Metapneumovirus. Também está associado à síndrome da cabeça inchada (SHS) em galinhas e é responsável por significativas perdas econômicas em sua produção. O presente estudo foi dividido em três partes. A primeira parte do trabalho consistiu em avaliar a beta-actina, gene utilizado como controle interno das técnicas moleculares de detecção viral, das células chicken embryo related (CER). Para isso, foi realizado o sequenciamento dos amplicons gerados pelo PCR do gene da beta-actina. A beta-actina das células BHK21 e CER foram detectadas utilizando oligonucleotídeos hamster-específicos. Além disso, pela análise filogenética as células CER e BHK21 apresentaram uma alta similaridade genética (p,0.996). Estes resultados sugerem que as células CER não deveriam ser mais consideradas como células ...

Ability, Avian Paramyxovirus, Diagnosis, Disease, Minor, Morbidity, Mortality, Newcastle Disease, Newcastle Disease Virus, PCR, Plays, Polymerase Chain Reaction, Poultry, Reverse Transcription, RNA, Role, Screening, Sensitivity, Specificity, Strain

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Lecture, 2017-11-11, Fika efter en forskare, Fika efter en forskare är en återkommande populärvetenskaplig seminarieserie på Vävens Kafé Fika.