Aim: To compare clonal T cell receptor γ (TCRγ) gene rearrangements in frozen and formalin-fixed paraffin wax-embedded (FFPE) tissue, using capillary electrophoresis for use in diagnostics, as T cell lymphomas may be difficult to diagnose by conventional methods.. Methods: The DNA for PCR was extracted from frozen and FFPE tissue, cell lines and blood. PCR primers Vγ1-8, Vγ9, Vγ10 or Vγ11 (5′ end labelled) combined with a mixture of JγP1/JγP/JγP2/Jγ2 (unlabelled) were used. Monoclonal cases were sequenced and clonality, reproducibility, sensitivity and specificity analyses were carried out.. Results: In all cases the molecular test was found to be in agreement with the histological diagnosis. Discrepancies were found between frozen and FFPE tissue in 18 of 56 (32%) tests. The method was highly reproducible. The sensitivity was found to be 0.5% for cell lines and 1% for patient specimens and the specificity 100%. The junctional region between the Vγ and Jγ segments was specific for ...
Aims In recent years the genetic aberrations associated with diffuse large B-cell lymphoma and the new subtype described in the 2008 revision of the WHO classification, B-cell lymphoma, unclassifiable, with features intermediate between diffuse large B-cell lymphoma and Burkitt lymphoma have been increasingly well defined. Recurrent genetic abnormalities include rearrangements involving MYC (8q24), BCL2 (18q21) and BCL6 (3q27); as the prognostic and therapeutic implications associated with these abnormalities are clarified their accurate identification at diagnosis is becoming increasingly critical. We describe our experience of using a panel of fluorescence in situ hybridisation (FISH) probes on formalin-fixed paraffin-embedded tissue sections in the diagnostic work-up of 162 patients with non-Burkitt high grade B-cell non-Hodgkins lymphomas (HG-BNHL). ...
A number of reports have recently emerged with focus on extraction of proteins from formalin-fixed paraffin-embedded (FFPE) tissues for MS analysis; however, reproducibility and robustness as compared to flash frozen controls is generally overlooked. The goal of this study was to identify and validate a practical and highly robust approach for the proteomics analysis of FFPE tissues. FFPE and matched frozen pancreatic tissues obtained from mice (n = 8) were analyzed using 1D-nanoLC-MS(MS)2 following work up with commercially available kits. The chosen approach for FFPE tissues was found to be highly comparable to that of frozen. In addition, the total number of unique peptides identified between the two groups was highly similar, with 958 identified for FFPE and 1070 identified for frozen, with protein identifications that corresponded by approximately 80%. This approach was then applied to archived human FFPE pancreatic cancer specimens (n = 11) as compared to uninvolved tissues (n = 8), where ...
Next generation sequencing (NGS) technologies have revolutionized cancer research allowing the comprehensive study of cancer using high throughput deep sequencing methodologies. These methods detect genomic alterations, nucleotide substitutions, insertions, deletions and copy number alterations. SOLiD (Sequencing by Oligonucleotide Ligation and Detection, Life Technologies) is a promising technology generating billions of 50 bp sequencing reads. This robust technique, successfully applied in gene identification, might be helpful in detecting novel genes associated with cancer initiation and progression using formalin fixed paraffin embedded (FFPE) tissue. This studys aim was to compare the validity of whole exome sequencing of fresh-frozen vs. FFPE tumor tissue by normalization to normal prostatic FFPE tissue, obtained from the same patient. One primary fresh-frozen sample, corresponding FFPE prostate cancer sample and matched adjacent normal prostatic tissue was subjected to exome sequencing. The
Next generation sequencing (NGS) technologies have revolutionized cancer research allowing the comprehensive study of cancer using high throughput deep sequencing methodologies. These methods detect genomic alterations, nucleotide substitutions, insertions, deletions and copy number alterations. SOLiD (Sequencing by Oligonucleotide Ligation and Detection, Life Technologies) is a promising technology generating billions of 50 bp sequencing reads. This robust technique, successfully applied in gene identification, might be helpful in detecting novel genes associated with cancer initiation and progression using formalin fixed paraffin embedded (FFPE) tissue. This studys aim was to compare the validity of whole exome sequencing of fresh-frozen vs. FFPE tumor tissue by normalization to normal prostatic FFPE tissue, obtained from the same patient. One primary fresh-frozen sample, corresponding FFPE prostate cancer sample and matched adjacent normal prostatic tissue was subjected to exome sequencing. The
FISH images of formalin fixed paraffin embedded sections of BAC. Red signals (white arrow heads) are indicative of the p53 gene probe, while green signals (yell
This video covers off on the challenging samples in cancer research, specifically formalin-fixed paraffin-embedded tissues. Presented by Beckman Coulter Life Sciences.
The promising results of anaplastic lymphoma kinase (ALK) inhibitors have changed the significance of ALK fusions in several types of cancer. These fusions are no longer mere research targets or diagnostic markers, but they are now directly linked to the therapeutic benefit of patients. However, most available tumor tissues in clinical settings are formalin-fixed and paraffin-embedded (FFPE), and this significantly limits detailed genetic studies in many clinical cases. Although recent technical improvements have allowed the analysis of some known mutations in FFPE tissues, identifying unknown fusion genes by using only FFPE tissues remains difficult. We developed a 5-rapid amplification of cDNA ends-based system optimized for FFPE tissues and evaluated this system on a lung cancer tissue with ALK rearrangement and without the 2 known ALK fusions EML4-ALK and KIF5B-ALK. With this system, we successfully identified a novel ALK fusion, KLC1-ALK. The result was confirmed by reverse transcription
COLOGNE, Germany - September 25, 2016: At the 28th Congress of the European Society of Pathology, Bruker today announces that it has expanded its license agreement with 3M for processing of FFPE tissue sections. Mass spectrometric imaging by MALDI-TOF (MALDI Imaging) is a technology with great potential for many applications in medicine. It is the leading mass spectrometric method for the analysis of tissues, such as Formalin Fixed Paraffin Embedded (FFPE) tissues. Bruker´s MALDI Tissuetyper™ solution makes this technology broadly applicable due to the unprecedented 10 kHz laser speed, robustness and ease of use of the underlying rapifleX™ MALDI-TOF mass spectrometer. The MALDI Tissuetyper enables anatomical pathology research based on multiplexed proteomic profiles and the spatial distribution of proteomic biomarkers on FFPE tissue sections. The MALDI Tissuetyper is used for the direct, label-free analysis of a wide range of analytes directly from tissue sections. It provides complementary ...
The storage and collection of fresh frozen tissue is by no means an easy task. Tissue sample collection and storage must conform to strict industry
Optimal reference genes for normalization of qRT-PCR data from archival formalin-fixed, paraffin-embedded breast tumors controlling for tumor cell content and decay of ...
This modified extraction protocol improves RNA and DNA yields from more precisely targeted regions of interest in histopathologic...
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Techniques for the extraction and use of nucleic acids from formalin‑fixed and paraffin-embedded (FFPE) tissues, preserved over long time periods in libraries, have been developed. However, DNA extracted from FFPE tissues is generally damaged, and long‑term storage may affect DNA quality. Therefore, it is important to elucidate the effect of long‑term storage on FFPE tissues and evaluate the techniques used to extract DNA from them. In the present study, the yield, purity, and integrity of DNA in FFPE tissue samples was evaluated. Two DNA extraction techniques were used: A silica‑binding DNA collection method using QIAamp DNA FFPE Tissue kit (QIA) and a total tissue DNA collection method using a WaxFree DNA extraction kit (WAX). A total of 25 FFPE tissues from lung adenocarcinomas were studied, which had been surgically resected and fixed at Okayama University Hospital prior to examination and subsequent storage at room temperature for 0.5, 3, 6, 9 and 12 years. Extracted DNA was ...
Sigma-Aldrich offers abstracts and full-text articles by [Anna Wojakowska, Łukasz Marczak, Karol Jelonek, Krzysztof Polanski, Piotr Widlak, Monika Pietrowska].
Hi colleague I use Dako polyclonal Myoglobin A0324 on formalin-fixed paraffin-embedded tissue. Dilution 1:1000 using Antibody-diluent Dako S2022 30 min. Link and label Dako LSAB K5001 each 25 min, chromogen DAB 10 min. Good luck Liv Liv Gr ntoft Departement of Forensic Medicin Universitets sjukhuset 581 85 Link ping Sweden phone +46 13 36 42 58 ...
Formalin-fixed paraffin-embedded (FFPE) archival clinical specimens are invaluable in discovery of prognostic and therapeutic targets for diseases such as cancer. However, the suitability of FFPE-derived genetic material for array-based comparative genomic hybridization (array-CGH) studies is underexplored. In this study, genetic profiles of matched FFPE and fresh-frozen specimens were examined to investigate DNA integrity differences between these sample types and determine the impact this may have on genetic profiles. Genomic DNA was extracted from three patient-matched FFPE and fresh-frozen clinical tissue samples. T47D breast cancer control cells were also grown in culture and processed to yield a fresh T47D sample, a fresh-frozen T47D sample and a FFPE T47D sample. DNA was extracted from all the samples; array-CGH conducted and genetic profiles of matched samples were then compared. A loss of high molecular weight DNA was observed in the FFPE clinical tissues and FFPE T47D samples. A dramatic
This study addresses two questions: (1) how does prevalence of prognostic biomarkers in old, archival paraffin-embedded tumor biopsy specimens obtained from 50 Asian, 50 black, and 50 white women diagnosed with breast cancer in Oakland, CCA between 1966 and 1990 compare across racial/ethnic groups and to that observed among recent paraffin-embedded specimens, and (2) what is the relationship of these biomarkers to survival, controlling for other biological and socio economic risk factors that affect survival? In Year 1, we have, as planned: (a) abstracted medical chart data on tumor characteristics and treatment for all 150 women, (b) appended these data to an existing database with the women? 5 sociodemographic and reproductive characteristics, (c) determined their vital status as of December 31, 1994, (d) located tumor blocks for 135 of these women, and (e) measured, by immunohistochemistry/image analysis, the following prognostic biomarkers: estrogen, progesterone, androgen, and epidermal growth
Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual
NanoStringNorm: an extensible R package for the pre-processing of NanoString mRNA and miRNA data: Motivation: The NanoString nCounter Platform is a new and promising technology for measuring nucleic acid abundances. It has several advantages over PCR-based techniques, including avoidance of amplification, direct sequence interrogation and digital detection for absolute quantification. These features minimize aspects of experimental error and hold promise for dealing with challenging experimental conditions such as archival formalin-fixed paraffin-embedded samples. However, systematic inter-sample technical artifacts caused by variability in sample preservation, bio-molecular extraction and platform fluctuations must be removed to ensure robust data.. Results: To facilitate this process and to address these issues for NanoString datasets, we have written a pre-processing package called NanoStringNorm in the R statistical language. Key features include an extensible environment for method ...
Background: Nowadays, molecular biomarkers have critical roles for cancer diagnosis and prognosis in clinical laboratories. Human papillomaviruses are the main agents for etiology of cervical carcinoma. The present survey was conducted to evaluate the genes methylation in cervical cancer and precancerous lesions involvement with HPV genotypes. Materials and Methods: C13orf18 and C1orf166 (MUL1 or Mulan) DNA methylation as potential biomarkers and risk factors was investigated in 112 liquid based cytology and Formalin-Fixed Paraffin-Embedded tissue specimens in Iranian females with cervical intraepithelial neoplasia and dysplasia. Results: In this survey, HPV18 (61.6%) and HPV16 (42.9%) proved to be the most common HPV genotypes identified by In-House Multiplex Real Time PCR. There were no significant relationship between HPV positivity and the methylated DNA genes mentioned above (p , 0.05). Conclusions: Our MethyLight data demonstrated that these genes could not be considered as specific, ...
Background: Mucins are the major secreted glycoproteins of the gastrointestinal tract including the colorectum. Aberrant expression of MUC4 has been observed in adenocarcinomas of pancreas, gallbladder, lungs, salivary glands, and epithelial ovarian carcinomas. However, the staining patterns of MUC4 and their clinical significance in colorectal adenocarcinomas (CRCs) have not been studied. Methods: One hundred and thirty two CRC patients, who underwent surgical resection and had not received any pre- or -post surgery therapy, were selected for this study. Formalin-fixed paraffin-embedded tissue specimens from these patients were evaluated for phenotypic expression of MUC4 using anti-human MUC4 rabbit polyclonal antibody and the horse-radish peroxidase detection method. The expression levels were correlated with key clinicopathologic features and patient outcomes. Results: MUC4 was localized to the cytoplasm in the normal colonic epithelium as well as in CRCs. Based on the cytoplasmic expression, ...
Adult Minimum: 6 mL whole blood in ONE pink top tube or TWO lavender top (EDTA) tubes. Children Minimum: 3 mL whole blood in lavender top (EDTA) tube. Optional specimen type: Formalin Fixed paraffin embedded tissue; Fresh Frozen tissue. Testing on smaller volumes than those requested will be attempted. However, in some cases, small blood volumes may compromise the ability to perform testing ...
The SeqPlex RNA Amplification Kit for whole genome transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) of extremely small quantities of degraded/highly fragmented RNA including non-polyA-tailed RNA. The yields from serum, single cells, RNA immunoprecipitation (RIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful NGS library preparation. The SeqPlex kit allows the user to pre-amplify these RNA samples while maintaining patterns of differential expression found in the unamplified sample, prior to entering an NGS workflow.. This kit is an extension of the WTA product line and has been developed to integrate with the Illumina® (GAIIx sequencer), SOLiD™ System (from Life Technologies), Ion Torrent (from Life Technologies) and other next generation sequencing workflows.. Features and Benefits. ...
promoter differs between paraffin-fixed and fresh-frozen tissues of glioblastoma (Hamilton et al., 2011). On evaluating 38 patients with GC, LINE-1 was reportedly more hypermethylated in FFPE tissues than in paired fresh-frozen tissues (Song et al., 2016).. Since the patient tissues were dissected for different purposes, they were preserved in various forms and given different pre-treatments. For pathological examination, GC tissues were fixed with formalin and embedded in paraffin. In contrast, for genomic analysis, tissues were immediately frozen in liquid nitrogen without fixation following gastrectomy. Bisulfite conversion can be affected by formalin fixation, as sequence artifacts such as cytosine deamination can be caused by formaldehyde (Do and Dobrovic, 2015). Therefore, we screened the LINE-1 methylation patterns in each tissue type separately to determine whether LINE-1 can be used as a marker under all circumstances. In the current study, we used samples from different patient groups, ...
Hello Jamie, A friend of mine stained xenographs with an anti-VEGF that recognizes both mouse and human VEGF. She purchased this antibody from Oncogene. Sorry it took so long, but my friend returned to Germany and it was difficult to get the information. Best Regards, Matthew Ogdie InnoGenex (925)543-1414 -----Original Message----- From: Jamie Erickson [mailto:[email protected]] Sent: Friday, December 03, 1999 8:10 AM To: [email protected]; [email protected] Subject: Re: CD31? Hi Denise, Im presently working on a mouse model of angiogenisis and I have been asked to try a variety of endothelial markers. I have been using vWF (von Willibrands factor), CD31 and v-cam-1 and I-cam-1. vWF stains endothelial cells very well in formalin fixed paraffin embedded mouse skin tissue. CD31 works on paraffin tissues but what I think the researcher need is a marker for neovascularization, something like a mouse VEGF which I dont know of any? So far I have not been able to shead light on the ...
NucleoSpin FFPE DNA is designed for the isolation and purification of total DNA from formalin-fixed, paraffin-embedded (FFPE) samples.
NucleoSpin FFPE DNA is designed for the isolation and purification of total DNA from formalin-fixed, paraffin-embedded (FFPE) samples.
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Our tissue repository contains thousands of paraffin tissue blocks, a large selection of tissue section slides of human cancer, normal tissue and rhesus normal fresh frozen tissue as well as paraffin blocks and slides. Tissue arrays (also Tissue MicroArrays or TMAs) contain up to 1000 tissue samples per microscope slide, allowing high-throughput analysis of RNA, DNA or protein molecules in diseases, which is particularly useful in biomarker research and drug target validation.
Background: To improve current treatment strategies for patients with aggressive colorectal cancer (CRC), the molecular understanding of subgroups of CRC with poor prognosis is of vast importance. SOX2 positive tumors have been associated with a poor patient outcome, but the functional role of SOX2 in CRC patient prognosis is still unclear. Methods: An in vitro cell culture model expressing SOX2 was used to investigate the functional role of SOX2 in CRC. In vitro findings were verified using RNA from fresh frozen tumor tissue or immunohistochemistry on formalin fixed paraffin embedded (FFPE) tumor tissue from a cohort of 445 CRC patients. Results: Using our in vitro model, we found that SOX2 expressing cells displayed several characteristics of cancer stem cells; such as a decreased proliferative rate, a spheroid growth pattern, and increased expression of stem cell markers CD24 and CD44. Cells expressing SOX2 also showed down-regulated expression of the intestinal epithelial marker CDX2. We ...
SR GROUP - Exporter, Importer, Manufacturer, Distributor, Supplier, Trading Company of DNA FFPE Tissue Kit Print based in Delhi, India
Gentaur molecular products has all kinds of products like :search , Panomics \ QuantiGene 2.0 Sample Processing Kit for FFPE Samples \ QS0108 for more molecular products just contact us
PRIMARY OBJECTIVES:. I. To determine the maximum-tolerated dose (MTD) of MLN8237 (alisertib) when given in combination with vorinostat and to select a dose and schedule for further testing (recommended Phase 2 dose: RP2D) in patients with lymphoid malignancies.. II. To describe the toxicities of MLN8237 when given in combination with vorinostat on a 21-day schedule.. III. To determine any clinical responses with MLN8237 in combination with vorinostat.. IV. To compare the plasma pharmacokinetics of MLN8237 when given alone and in combination with vorinostat.. V. To perform immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) analysis to determine aurora kinase A (AURKA) expression in archival formalin-fixed paraffin-embedded sections from the most recent available tumor specimens of patients.. VI. To perform correlative studies for apoptosis and proliferation on bone marrow and lymph node specimens, where available, obtained from patients in the expanded cohort at ...
PRIMARY OBJECTIVES:. I. To determine the maximum-tolerated dose (MTD) of MLN8237 (alisertib) when given in combination with vorinostat and to select a dose and schedule for further testing (recommended Phase 2 dose: RP2D) in patients with lymphoid malignancies.. II. To describe the toxicities of MLN8237 when given in combination with vorinostat on a 21-day schedule.. III. To determine any clinical responses with MLN8237 in combination with vorinostat.. IV. To compare the plasma pharmacokinetics of MLN8237 when given alone and in combination with vorinostat.. V. To perform immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) analysis to determine aurora kinase A (AURKA) expression in archival formalin-fixed paraffin-embedded sections from the most recent available tumor specimens of patients.. VI. To perform correlative studies for apoptosis and proliferation on bone marrow and lymph node specimens, where available, obtained from patients in the expanded cohort at ...
Immunohistochemical analysis of Granzyme H staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX ...
Gerdes MJ, Sevinsky CJ, Sood A, Adak S, Bello MO, Bordwell A, Can A, Corwin A, Dinn S, Filkins RJ, Hollman D, Kamath V, Kaanumalle S, Kenny K, Larsen M, Lazare M, Li Q, Lowes C, McCulloch CC, McDonough E, Montalto MC, Pang Z, Rittscher J, Santamaria-Pang A, Sarachan BD, Seel ML, Seppo A, Shaikh K, Sui Y, Zhang J, Ginty F et al. 2013. Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue. Proc Natl Acad Sci U S A, 110 (29), pp. 11982-11987. , Show Abstract , Read more Limitations on the number of unique protein and DNA molecules that can be characterized microscopically in a single tissue specimen impede advances in understanding the biological basis of health and disease. Here we present a multiplexed fluorescence microscopy method (MxIF) for quantitative, single-cell, and subcellular characterization of multiple analytes in formalin-fixed paraffin-embedded tissue. Chemical inactivation of fluorescent dyes after each image acquisition round allows reuse of ...
Gerdes MJ, Sevinsky CJ, Sood A, Adak S, Bello MO, Bordwell A, Can A, Corwin A, Dinn S, Filkins RJ, Hollman D, Kamath V, Kaanumalle S, Kenny K, Larsen M, Lazare M, Li Q, Lowes C, McCulloch CC, McDonough E, Montalto MC, Pang Z, Rittscher J, Santamaria-Pang A, Sarachan BD, Seel ML, Seppo A, Shaikh K, Sui Y, Zhang J, Ginty F et al. 2013. Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue. Proc Natl Acad Sci U S A, 110 (29), pp. 11982-11987. , Citations: 112 (Scopus) , Show Abstract , Read more Limitations on the number of unique protein and DNA molecules that can be characterized microscopically in a single tissue specimen impede advances in understanding the biological basis of health and disease. Here we present a multiplexed fluorescence microscopy method (MxIF) for quantitative, single-cell, and subcellular characterization of multiple analytes in formalin-fixed paraffin-embedded tissue. Chemical inactivation of fluorescent dyes after each image ...
This product is intended for use in immunohistochemical (IHC) staining on formalin fixed paraffin embedded (FFPE) tissue sections. This product inhibits nonspecific staining due to endogenous biotin during the IHC detection of antigens. CE ...
The 3D-Gene™ technology provides a new dimension in mRNA and miRNA microarray sensitivity and reproducibility.. 3D-Gene™ DNA chips utilize patented resin slides to minimize background noise and microbead agitation technology to improve hybridization. Its unique 3D micro column structure define each spot to 100 µm in diameter for increased accuracy. It ensures a 100-fold increase in sensitivity and is ideal for challenging samples and low abundance targets. 3D-Gene™ is thus ideal for analyses of formalin fixed paraffin embedded (FFPE) tissue samples.. This innovative technology also makes it possible to measure genes expressed at very low levels that might be undetected by other array platforms on the market.. 3D-Gene™ Human miRNA chips are constructed using the latest information available from the Wellcome Trust Sanger institute / University of Manchester miRNA database (miRBase).. ...
Thus, the SEER registries provide a unique opportunity for performing biospecimen studies on a representative sample of cancer cases from a particular geographic area. Recognizing this potential, the SEER Residual Tissue Repository (RTR) program was established in 2003.. The RTR program aims to retain specimens associated with SEER patients that would otherwise be discarded. Investigators can use these specimens for research on prognostic biomarkers, etiology, and other hypotheses relevant to the population-based sample.. Most RTR biospecimens are formalin-fixed paraffin-embedded tissue blocks; however, some other biospecimens are maintained within the RTR, including a pancreatic tissue microarray. The RTR:. ...
The Tissue Management Shared Resource was established to provide IRB-approved centralized tissue procurement service for specimens derived from consented human participants.. More than 23,000 bodily fluids such as blood, urine, and pleural fluid and tissue specimens including breast, lung, prostate, kidney, bladder, colon, liver, and pancreas have been collected since its establishment. Each tissue aliquot is evaluated by a board-certified anatomical pathologist to determine tumor cellularity. The resource has both frozen and formalin-fixed paraffin-embedded tissue available for research projects. Specimen-related data are captured and stored in our tissue management database, OpenSpecimen, and are correlated with clinical information. In parallel with the banking effort, the Tissue Management Shared Resource offers prospective procurement services for fresh tissue. The prospective procurement is coordinated with the surgical staff, pathologists, and investigators. The Tissue Management Resource ...
Introduction: Anaplastic pancreatic cancer (APC) is a rare subtype of pancreatic ductal adenocarcinoma (PDA). The prognosis of APC is poorer than that of ordinary PDA. There is no established therapeutic strategy as well as PDA. In this study, we compared the expression of molecular markers in intratumoral different lesions which are undifferentiated lesion (UL) and ductal lesion (DL) to reveal progression pattern of APC and hold important clue for the therapy of APC.. Materials & Methods: Formalin fixed paraffin embedded blocks were made from the primary APC tissues obtained from 6 patients. Each block was manipulated for staining with Hematoxylin and Eosin (H&E) and immunohistochemistry (IHC) for SOX9, E-cadherin, vimentin, ZEB-1, Snail, N-cadherin, CD24 and CD44. Using H&E slice, each sample was divided from cancerous lesion and non-cancerous lesion. Furthermore, cancerous lesion were divided from DL and UL. And then, for each marker, the intensity of staining of UL was compared with that of ...
Targeted cancer therapies are defined as antibody or small molecule drugs that block the growth and spread of cancer by interfering with specific cell molecules involved in tumor growth and progression. Multiple targeted therapies have been approved by the FDA for treatment of specific cancers. Molecular genetic profiling is often needed to identify targets amenable to targeted therapies and to minimize treatment costs and therapy-associated risks.. Next-generation sequencing has recently emerged as an accurate, cost-effective method to identify alterations across numerous genes known to be associated with response or resistance to specific targeted therapies. This is a single assay that uses formalin-fixed paraffin-embedded tissue or cytology slides to assess for common somatic mutations and rearrangements (fusions) involving 11 genes known to be associated with lung cancer. The results of this test can be useful for assessing prognosis and guiding treatment of individuals with lung tumors. ...
Purification kits for RNA from a variety of sources, including tissue, formalin-fixed paraffin-embedded samples, blood, plants, soil, and food. These kits can quickly and easily provide high-quality and pure RNA.
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Our research relies on equipments including barocyclers, mass spectrometers and high performance computing systems.. We developed PCT-SWATH/DIA technology which allows fast, reproducible and cheap proteomic analysis of tissue specimens including fresh tissues and formalin-fixed paraffin-embedded (FFPE) tissues. With no more than 1 mg tissue, the PCT method produces on average 50 µg peptides, while only 0.2-1µg peptides are sufficient for an optimal generation of a SWATH/DIA map using TripleTOF (Shao, et al. 2015) or the latest Orbitraps such as Q-Exactive HF/HFX, Fusion and Lumos within 0.5-2 hrs. We also worked with Pressure Biosciences Inc in developing PCT-MicroPestle for enhanced sample preparation efficiency (Shao, et al. 2016).. ...