Melanin in the dimorphic fungal pathogen Paracoccidioides brasiliensis: effects on phagocytosis, intracellular resistance and...
The fungal pathogen Paracoccidioides brasiliensis produces a melanin-like pigment in the presence of l-DOPA in vitro. We investigated whether melanization affected yeast uptake by alveolar and peritoneal macrophages, the intracellular resistance of fungal cells and their susceptibility to antifungal drugs. The interactions of melanized and nonmelanized P. brasiliensis with murine primary macrophages and J774.16 and MH-S macrophage-like cell lines were investigated. Melanized yeast cells were poorly phagocytosed by the cells even in the presence of complement. Melanization caused significant interference with the binding of cell wall components to lectin receptors on macrophages. Melanized cells were also more resistant than nonmelanized cells to the antifungal activity of murine macrophages. No difference in the susceptibilities of melanized and nonmelanized P. brasiliensis to antifungal drugs was observed using the minimum inhibitory concentration (MIC) method. However killing assays showed ...
Paracoccidioides brasiliensis | definition of Paracoccidioides brasiliensis by Medical dictionary
Looking for online definition of Paracoccidioides brasiliensis in the Medical Dictionary? Paracoccidioides brasiliensis explanation free. What is Paracoccidioides brasiliensis? Meaning of Paracoccidioides brasiliensis medical term. What does Paracoccidioides brasiliensis mean?
Miltefosine is fungicidal to Paracoccidioides spp. yeast cells but subinhibitory concentrations induce melanisation<...
TY - JOUR. T1 - Miltefosine is fungicidal to Paracoccidioides spp. yeast cells but subinhibitory concentrations induce melanisation. AU - Rossi, Diego Conrado Pereira. AU - Spadari, Cristina de Castro. AU - Nosanchuk, Joshua D.. AU - Taborda, Carlos Pelleschi. AU - Ishida, Kelly. PY - 2017/4/1. Y1 - 2017/4/1. N2 - Paracoccidioidomycosis (PCM) is a systemic mycosis caused by the dimorphic fungi Paracoccidioides spp. The duration of antifungal treatment ranges from months to years and relapses may nevertheless occur despite protracted therapy. Thus, there remains an urgent need for new therapeutic options. Miltefosine (MLT), an analogue of alkylphospholipids, has antifungal activity against species of yeast and filamentous fungi. The aim of this study was to evaluate the antifungal effects of MLT on the yeast forms of Paracoccidioides brasiliensis and Paracoccidioides lutzii. MLT demonstrated inhibitory activity from 0.12 to 1 µg/mL, which was similar to amphotericin B or the combination ...
Scholarship 14/08987-1 - Paracoccidioides brasiliensis, Paracoccidioidomicose - BV FAPESP
Study of the role of secreted aspartyl protease (PbSAP) virulence of the pathogenic fungus Paracoccidioides brasiliensis. Scholarships in Brazil Doctorate. Daniele Gonçalves Castilho. Biological Sciences. scholarship by fapesp
Functional analysis of Paracoccidioides brasiliensis 14-3-3 adhesin expressed in Saccharomyces cerevisiae | BMC Microbiology |...
14-3-3 proteins comprise a family of eukaryotic multifunctional proteins involved in several cellular processes. The Pb14-3-3 of Paracoccidioides brasiliensis seems to play an important role in the Paracoccidioides-host interaction. Paracoccidioides brasiliensis is an etiological agent of paracoccidioidomycosis, which is a systemic mycosis that is endemic in Latin America. In the initial steps of the infection, Paracoccidioides spp. synthetizes adhesins that allow it to adhere and invade host cells. Therefore, the aim of this work was to perform a functional analysis of Pb14-3-3 using Saccharomyces cerevisiae as a model. The functional analysis of Pb14-3-3 was performed in S. cerevisiae, and it was found that Pb14-3-3 partially complemented S. cerevisiae proteins Bmh1p and Bmh2p, which are recognized as two yeast 14-3-3 homologues. When we evaluated the adhesion profile of S. cerevisiae transformants, Pb14-3-3 acted as an adhesin in S. cerevisiae; however, Bmh1p did not show this function. The influence
Paracoccidioides lutzii Teixeira et al. ATCC ® MYA-826D-2™
Paracoccidioides lutzii ATCC ® MYA-826D-2™ Designation: Genomic DNA from Paracoccidioides lutzii strain Pb01 [ATCC ® MYA-826™] Application:
Virulence insights from the Paracoccidioides brasiliensis transcriptome
The P. brasiliensis virulence orthologs were placed in groups, based either on their functional or structural characteristics, such as: metabolism-, cell wall-, detoxification-related genes, secreted factors and other determinants (Navarro-Garcia et al., 2001). In this review, we examined specific gene products that were assigned as virulence factors by a molecular genetic approach (molecular Kochs postulates).. METABOLISM-RELATED GENES. P. brasiliensis, a facultative intracellular pathogen, is able to survive and replicate within the phagosome of nonactivated murine and human macrophages (Brummer et al., 1988a,b, 1989, 1990; Moscardi-Bacchi et al., 1994; Gonzalez et al., 2000). Thus, this fungus may have evolved mechanisms that counteract the metabolic constraints imposed by phagocytic cells. The phagosome is believed to be a poor source of complex carbon, such as carbohydrates. Instead, intracellular pathogens may find only two-carbon (C2) compounds for energy production such as acetate, a ...
Paracoccidioides brasiliensis Disseminated Disease in a Patient with Inherited Deficiency in the β1 Subunit of the Interleukin ...
Background. Paracoccidioides brasiliensis is a facultative intracellular dimorphic fungus that causes paracoccidioidomycosis (PCM), the most important deep mycosis in Latin America. Only a small percentage of individuals infected by P. brasiliensis develop clinical PCM, possibly in part because of genetically determined interindividual variability of host immunity. However, no primary immunodeficiency has ever been associated with PCM.. Methods. We describe the first patient, to our knowledge, with PCM and a well-defined primary immunodeficiency in the β1 subunit of the interleukin (IL)-12/IL-23 receptor, a disorder previously shown to be specifically associated with impaired interferon (IFN)-γ production, mycobacteriosis, and salmonellosis.. Results. Our patient had a childhood history of bacille Calmette-Guùrin disease and nontyphoid salmonellosis and, at the age of 20 years, presented to our clinic with a disseminated (acute) form of PCM. He responded well to antifungal treatment and is ...
Paracoccidioides Species - Doctor Fungus
Paracoccidioides brasiliensis is the causative agent of a true systemic (endemic) mycosis called paracoccidioidomycosis. The spectrum of the disease is wide, varying from an asymptomatic infection verified by the skin test to a subclinical, symptomatic or chronic infection. Diagnosis of paracoccidioidomycosis is often difficult. The infection may become apparent several years after exposure to Paracoccidioides brasiliensis, suggesting the possibility of a long latent period. The infection is presumably acquired via inhalation of the conidia, followed by primary infection of the lungs. The degree of pathogenicity may vary and appears strain-dependent. An exocellular serine-thiol proteinase enzyme is a significant virulence factor of the fungus for tissue invasion. In addition to the primary pulmonary form of the disease, acute pulmonary, chronic pulmonary, and disseminated forms may also be observed. In cases of disseminated paracoccidioidomycosis, reticuloendothelial system, skin and mucous ...
Phenotypic and Functional Characterization of NK Cells in Human Immune Response against the Dimorphic Fungus Paracoccidioides...
The major aim of this study was to evaluate the possible role of NK cells in the initial immunological response against P. brasiliensis. Among the few studies about this issue, the study carried out by Peraçoli et al. (13) showed more NK cells in the peripheral blood of PCM patients, although exhibiting a diminished cytotoxic activity. Our data partially confirm these data, given that we observed that NK cells from patients with active disease did exhibit a decreased cytotoxic response against target cells. In relation to the number of CD56+ cells, we found no differences between PCM patients and healthy individuals. Additionally, we observed that after the treatment, the cytotoxic capability of NK cells from patients was similar to that observed in the control group. These data indicate that during the development of the disease, it is possible that the acquired immunological response interfere with the activation of NK cells. PCM patients with active disease are characterized by the ...
Antibody Response to the 43 kDa Glycoprotein of Paracoccidioides brasiliensis as a Marker for the Evaluation of Patients under...
Abstract
Sera of patients with paracoccidioidomycosis contained IgG-, IgA-, and IgM-specific antibodies to a 43 kDa antigen contained in the filtrate of a culture of Paracoccidioides brasiliensis. IgG- and IgA-specific antibodies were present in all observed patients. The IgM response was more frequent in acute cases, and the mean titers of IgG- and IgM-specific antibodies were higher in the acute forms. By the fourth month of chemotherapy, there was a decay of IgG, IgA, and IgM antibody titers to this antigen in acute cases, correlating with clinical improvement. The detection of IgG and IgA antibodies and the sequential determination of antibodies to the 43 kDa glycoprotein may be useful tools for serodiagnosis and evaluation of therapeutic efficacy.
43-kilodalton glycoprotein from Paracoccidioides brasiliensis: immunochemical reactions with sera from patients with...
Sera from patients with paracoccidioidomycosis (PCM), histoplasmosis (HP), or Jorge Lobos disease (JL) were titrated against purified gp43 from Paracoccidioides brasiliensis by using both enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation (IPP) reactions with 125I-labeled antigens. In IPP, PCM sera and other sera could be distinguished on the basis of serum titers, whereas in ELISA, 53% of the HP sera and 29% of the JL sera reacted similarly to the PCM sera. To investigate the possible role of the carbohydrate epitopes in these reactions, we compared the reactivities of sera from several patients with native and deglycosylated gp43. Competition experiments were carried out with monosaccharides as inhibitors. The results suggest that greater than 85% of the reactions of the PCM sera with gp43 involved peptide epitopes. Cross-reactions with HP and JL sera in ELISA were predominantly attributed to periodate-sensitive carbohydrate epitopes containing galactosyl residues. HP and JL ...
The homeostasis of iron, copper, and zinc in Paracoccidioides brasiliensis, Cryptococcus neoformans var. Grubii, and...
TY - JOUR. T1 - The homeostasis of iron, copper, and zinc in Paracoccidioides brasiliensis, Cryptococcus neoformans var. Grubii, and Cryptococcus gattii. T2 - A comparative analysis. AU - Silva, Mirelle Garcia. AU - Schrank, Augusto. AU - Bailão, Elisa Flávia L C. AU - Bailão, Alexandre Melo. AU - Borges, Clayton Luiz. AU - Staats, Charley Christian. AU - Parente, Juliana Alves. AU - Pereira, Maristela. AU - Salem-Izacc, Silvia Maria. AU - Mendes-Giannini, Maria José Soares. AU - Oliveira, Rosely Maria Zancopé. AU - Silva, Lívia Kmetzsch Rosa e. AU - Nosanchuk, Joshua D.. AU - Vainstein, Marilene Henning. AU - Soares, Célia Maria de Almeida. PY - 2011. Y1 - 2011. N2 - Iron, copper, and zinc are essential for all living organisms. Moreover, the homeostasis of these metals is vital to microorganisms during pathogenic interactions with a host. Most pathogens have developed specific mechanisms for the uptake of micronutrients from their hosts in order to counteract the low availability of ...
Functional analysis of Paracoccidioides brasiliensis 14-3-3 adhesin expressed in Saccharomyces cerevisiae - pdf descargar
Functional analysis of Paracoccidioides brasiliensis 14-3-3 adhesin expressed in Saccharomyces cerevisiae. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
ReP USP - Detalhe do registro: Analysis of the interaction between Paracoccidioides brasiliensis and macrophages through of...
ROMERA, Lavínia Maria DalMas; ALMEIDA, Sandro Rogério de. Analysis of the interaction between Paracoccidioides brasiliensis and macrophages through of notch receptores. Brazilian Journal of Pharmaceutical Sciences[S.l: s.n.], 2011 ...
Paracoccidioides brasiliensis (Splendore) Almeida, anamorph ATCC ®
Paracoccidioides brasiliensis ATCC ® 14283™ Designation: NIH 6901 Application: Biomedical Research and Development Material
Paracoccidioides brasilienses isolates obtained from patients with acute and chronic disease exhibit morphological differences...
The basis for virulence in Paracoccidioides brasiliensis is not completely understood. There is a consensus that the sequencial in vitro subcultivation of P. brasiliensis leads to loss of its pathogenicity, which can be reverted by reisolation from animal passage. Attention to morphological and biochemical properties that are regained or demonstrated after animal passage may provide new insights into factors related to the pathogenicity and virulence of P. brasiliensis. We evaluated morphological characters: the percentage of budding cells, number of buds by cell and the diameter of 100 mother cells of yeast-like cells of 30 P. brasiliensis isolates, before and after animal passage. The isolates were obtained from patients with different clinical forms of paracoccidioidomycosis (PCM): acute form (group A, n=15) and chronic form (group C, n=15). The measurement of the yeast cell sizes was carried out with the aid of an Olympus CBB microscope coupled with a micrometer disc. We measured the major ...
Synthesis and immunological activity of a branched peptide construction carrying the T cell epitope of Paracoccidioides...
2Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, Brazil. The 43,000 kDa glycoprotein, gp43, is a major immunodominant antigen of Paracoccidioides brasiliensis. It has been used as a diagnostic reagent of paracoccidioidomycosis both in serological reactions and skin tests. The gp43 was cloned and completely sequenced [Cisalpino et al. 1996. J Biol Chem 271: 4553] and the structure of its single high-mannose carbohydrate chain determined [Almeida et al. 1997. Glycobiology 6: 507]. Besides carrying B cell epitopes the gp43 also mediates cellular immune reactions [Rodrigues & Travassos 1994. J Med Vet Mycol 32: 77]. Its T cell epitope was mapped to a 15-amino acid peptide (P10). Peptide 10 not only stimulates the in vitro lymphoproliferation of primed lymph node cells from sensitized or infected mice, but its immunization with CFA in mice followed by an intra-tracheal challenge with a virulent strain of P. brasiliensis is protective, significantly reducing the number of ...
iAH Search interface 2.4 - Results of the search |page 1|
Cavalcanti, Sarah Desir e Barbosa et al. Analysis of the genetic polymorphism of Paracoccidioides brasiliensis and Paracoccidioides cerebriformis Moore by random amplified polymorphic DNA (RAPD) and 28S ribosomal DNA sequencing: Paracoccidioides cerebriformis revisited. Rev. Inst. Med. trop. S. Paulo, June 2005, vol.47, no.3, p.119-123. ISSN 0036- ...
Binding of Paracoccidioides brasiliensis to laminin through surface glycoprotein gp43 leads to enhancement of fungal...
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Cell survival and altered gene expression following photodynamic inactivation of Paracoccidioides brasiliensis. | Sigma-Aldrich
Sigma-Aldrich offers abstracts and full-text articles by [Luciane M Almeida, Fabiana F Zanoelo, Kelly P Castro, Iouri E Borissevitch, Célia M A Soares, Pablo J Gonçalves].
Histopathology of Paracoccidioidomycotic Infection in Athymic and Euthymic Mice: A Sequential Study | The American Journal of...
Abstract
We report the comparative and sequential histologic analysis of lesions developed by Paracoccidioides brasiliensis-infected athymic and euthymic BALB/c mice, using conventional and specific stain to characterize the morphology of fungi and inflammatory cells as well as the extracellular matrix patterns. In both groups, neutrophils and macrophages were the predominant cells; macrophage transition to giant and epithelioid cells was observed. Degenerated fungi and fungal dust were associated with local neutrophil infiltration and the presence of pseudoxantomatous macrophages. Paracoccidioides brasiliensis are always found within an extracellular matrix microenvironment. The main differences between lesions developed by nu/+ and nude (nu/nu) mice resided in the more pronounced encapsulating tendency in the former ones. In both groups of animals, lesions in several organs were associated with extensive hemopoiesis. At the first week of infection, there was evidence of fungal destruction and control
Repositorio da Producao Cientifica e Intelectual da Unicamp: Enhanced Production Of Specific Igg4, Ige, Iga And Tgf-beta In...
Paracoccidioidomycosis (PCM) occurs in two distinct forms, the acute or juvenile form (JF), and the chronic or adult form (AF). To clarify the basis of this dichotomy, specific IgG subclasses, IgA and IgE anti-gp43 were measured by enzyme-linked immunosorbent assay, in patients with different forms of PCM. Serum levels of tumor necrosis factor-alpha, interleukin (IL)-6, IL-8, macrophage inflammatory protein (MIP)-1alpha and transforming growth factor (TGF)-beta were also quantified. We show here that JF patients have significantly higher titers of IgE antibodies against gp43, an immunodominant antigen specific for Paracoccidioides brasiliensis, than do patients with the unifocal adult form (UF-AF, isolated lesions). Patients with the multifocal adult form (MF-AF, lesions in more than one organ) also produced elevated levels of anti-P. brasiliensis IgE. Furthermore, specific IgE levels were correlated with IgG4, IgA and eosinophilia. Patients with JF showed eosinophilia and increased levels of ...
peptides international - LUnivers Académique de la Recherche à Montpellier
La paracoccidioidomycose (PCM) est une an infection fongique systémique potentiellement mortelle causée par des membres du complexe Paracoccidioides brasiliensis et P. lutzii. Les diagnostics de routine du PCM jusquau niveau Read More …. ...
Repositorio da Producao Cientifica e Intelectual da Unicamp: Avaliação da capacidade de indução de diferenciação de células...
Resumo: A paracoccidioidomicose (PCM), causada pelo fungo dimórfico Paracoccidioides brasiliensis (Pb), é a micose sistêmica de maior incidência no Brasil. Estudos anteriores demonstraram que a resistência ou suscetibilidade a essa infecção podem ser associadas, respectivamente, a padrões de resposta Th1 ou Th2. Recentemente, foram descritas novas subpopulações de linfócitos T, dentre elas as células Th17, que tem se mostrado importantes na proteção contra infecções fúngicas, mas cujo papel ainda não foi estudado na PCM humana. A diferenciação de células T CD4+ é modulada após o reconhecimento do patógeno por células dendríticas (DCs) por meio de vários receptores de reconhecimento padrão (PRRs), os quais modulam a diferenciação de células Th1, Th2 e Th17. Neste trabalho investigamos o papel dos receptores TLR2, TLR4 e Dectina-1 no reconhecimento de células leveduriformes de P. brasiliensis (Pb) por DCs derivadas de monócitos, assim como, a capacidade dessas DCs ...
SWISS-MODEL Repository | C1GTI3
SWISS-MODEL Repository entry for C1GTI3 (PFF1_PARBA), Vacuolar membrane protease. Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioidesbrasiliensis)
Description: Desenvolvimento de método para diagnóstico da paracoccidioidomicose humana utilizando antígeno recombinante de...
Paracoccidioidomicose (PCM) é uma micose sistêmica causada por Paracoccidioides brasiliensis. É considerada a micose sistêmica de maior prevalência na América do Sul, principalmente no Brasil, o qual apresenta o maior número de casos. A glicoproteína de 43 kDa, gp43, é o principal antígeno utilizado no diagnóstico e epidemiologia da PCM. O objetivo deste trabalho foi produzir gp43 recombinante na forma solúvel para utilização no diagnóstico de PCM humana. Para a produção da proteína recombinante, primeiramente o gene gp43 foi clonado em vetor de expressão pETTEV. Algumas construções do gene neste vetor foram feitas com a finalidade de obter melhor expressão e solubilidade da proteína. Para isso, foram realizadas estratégias para deleção de 20 aminoácidos das regiões N-terminal e/ou C-terminal. A proteína, com deleção na região N-terminal (gp43ΔNt), foi produzida em E. coli BL21 e purificada por cromatografia de afinidade. Sua reatividade foi avaliada ...
Research Grants 11/22567-7 - Biological Sciences, Microbiology - BV FAPESP
Prophylactic and therapeutic vaccination using dendritic cells primed with peptide 10 derived from the 43 kDa glycoprotein of Paracoccidioides Brasiliensis. Regular Research Grants Publications Scientific article. Carlos Pelleschi Taborda. Microbiology
Yeast-form wall protein elisa and antibody
Shop Yeast-form wall protein ELISA Kit, Recombinant Protein and Yeast-form wall protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Paracoccidioidomycosis (PCM) is an endemic systemic mycosis in Latin America, with | Discovery of Serotonin N-acetyltransferase...
Paracoccidioidomycosis (PCM) is an endemic systemic mycosis in Latin America, with the best prevalence in Brazil, Colombia, and Venezuela. most significant systemic granulomatous illnesses in Latin America (Taborda et al., 2015). PCM is certainly widespread in Brazil especially, affecting generally rural employees (Restrepo, 1985; Taborda and Travassos, 2012b). In Brazil, 1 approximately,853 (51.2%) of 3,583 confirmed deaths due to systemic mycoses from 1996 to 2006 were caused by PCM (Prado et al., 2009). Phylogenetic analyses have revealed that this clinically relevant species include (Teixeira et al., 2013). Acquisition of spp. follows the inhalation of conidia, which are deposited into the lower respiratory tract. These propagules subsequently undergo morphogenic transformation into yeast forms, which constitute the pathogenic morphology EM9 in tissues (Taborda et al., 2015). PCM has two main clinical forms that are predicated upon the immunological status of the infected host. Within weeks ...
Antibodies against glycolipids enhance antifungal activity of macrophages and reduce fungal burden after infection with <i...
TY - JOUR. T1 - Antibodies against glycolipids enhance antifungal activity of macrophages and reduce fungal burden after infection with Paracoccidioides brasiliensis. AU - Bueno, Renata A.. AU - Thomaz, Luciana. AU - Muñoz, Julian E.. AU - Da Silva, Cássia J.. AU - Nosanchuk, Joshua D.. AU - Pinto, Márcia R.. AU - Travassos, Luiz R.. AU - Taborda, Carlos P.. N1 - Funding Information: The present work was supported by Capes and FAPESP grant 2011/17267-4, 2013/18655-3. CT and LT are career research fellows of the CNPq. Publisher Copyright: © 2016 Bueno, Thomaz, Muñoz, da Silva, Nosanchuk, Pinto, Travassos and Taborda. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.. PY - 2016/2/3. Y1 - 2016/2/3. N2 - Paracoccidioidomycosis is a fungal disease endemic in Latin America. Polyclonal antibodies to acidic glycosphingolipids (GSLs) from Paracoccidioides brasiliensis opsonized yeast forms in vitro increasing phagocytosis and reduced the fungal burden of infected animals. Antibodies to ...
UniProt: C1GVY4 PARBA
ID C1GVY4_PARBA Unreviewed; 857 AA. AC C1GVY4; DT 26-MAY-2009, integrated into UniProtKB/TrEMBL. DT 04-FEB-2015, sequence version 2. DT 07-JUN-2017, entry version 36. DE RecName: Full=V-type proton ATPase subunit a {ECO:0000256,RuleBase:RU361189}; GN ORFNames=PAAG_02679 {ECO:0000313,EMBL:EEH40703.2}; OS Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides OS brasiliensis). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Onygenales; Onygenales incertae sedis; OC Paracoccidioides. OX NCBI_TaxID=502779 {ECO:0000313,EMBL:EEH40703.2, ECO:0000313,Proteomes:UP000002059}; RN [1] {ECO:0000313,EMBL:EEH40703.2, ECO:0000313,Proteomes:UP000002059} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC MYA-826 / Pb01 {ECO:0000313,Proteomes:UP000002059}; RX PubMed=22046142; DOI=10.1371/journal.pgen.1002345; RA Desjardins C.A., Champion M.D., Holder J.W., Muszewska A., RA Goldberg J., Bailao A.M., Brigido M.M., Ferreira M.E., Garcia ...
Poly(lactic acid-glycolic acid) nanoparticles markedly improve immunological protection provided by peptide P10 against murine...
Background and purpose:The present study reports on the preparation and testing of a sustained delivery system for the immunomodulatory peptide P10 aimed at reducing the in vivo degradation of the peptide and the amount required to elicit a protective immune response against paracoccidioidomycosis.Experimental approach:BALB/c mice were infected with the yeast Paracoccidioides brasiliensis to mimic the chronic form of paracoccidioidomycosis. the animals were treated daily with sulfamethoxazole/trimethoprim alone or combined with peptide P10, either emulsified in Freunds adjuvant or entrapped in poly(lactic acid-glycolic acid) (PLGA) nanoparticles at different concentrations (1 mu g, 5 mu g, 10 mu g, 20 mu g or 40 mu g center dot 50 mu L-1). Therapeutic efficacy was assessed as fungal burden in tissues and the immune response by quantitative determination of cytokines.Key results:Animals given combined chemotherapy and P10 nanotherapy presented a marked reduction of fungal load in the lungs, ...
Microimmunodiffusion test for the serodiagnosis of paracoccidioidomycosis - CORE
We used the micro- and macroimmunodiffusion test for the qualitative and quantitative measurement of anti - P. brasiliensis antibodies in serum of patients with paracoccidioidomycosis. All 103 paracoccidioidomycosis sera (100%) were positive in the micro test versus 87% positivity index in the macrotest. All 83 control sera from patients with other diseases were negative in both tests. Titers of the positive sera tended to be higher in the microtest, which revealed sharper and easier to read precipiting bands. Microimmunodiffusion is simple to be performed, requires a minimum amount of reagents and allows the simultaneous testing of 102 sera. It may replace the macrotest specially in laboratories dealing with great serologic routine ...
Regulation of immune responses by T suppressor cells and by serum in chronic paracoccidioidomycosis<...
TY - JOUR. T1 - Regulation of immune responses by T suppressor cells and by serum in chronic paracoccidioidomycosis. AU - Castaneda, Elizabeth. AU - Brummer, Elmer. AU - Pappagianis, Demosthenes. AU - Stevens, David A.. PY - 1988. Y1 - 1988. N2 - Regulation of cellular responses was studied during the course of chronic murine disseminated paracoccidioidomycosis. Regulation of peripheral blood lymphocyte (PBL) proliferative responses to concanavalin A (Con A) was studied in vitro by mixing PBL from infected and noninfected mice. PBL from mice infected for 18 weeks had depressed responses to Con A and they depressed the Con A responses of PBL from noninfected mice by 95% when they were mixed in a 1:1 ratio. After treatment of PBL from infected mice with anti-Lyt-2.2 antibody plus complement, the responses to Con A were increased to normal values. The percentage of T-cell subpopulations in PBL from infected mice did not differ significantly from those of normal mice. Immunoregulation of ...
Paracoccidioidomycosis - Net Health Book
With paracoccidioidomycosis spores get inhaled into the lungs and the disease spreads through the blood to mucous membranes of the mouth and to the skin.
Stem Cell Proliferation - IGF-1 modulates gene expression of proteins
The 43-kDa glycoprotein of may be the major diagnostic antigen of paracoccidioidomycosis, the prevalent systemic mycosis of Latin America. challenge by virulent antigens, whether associated with polyclonal B-cell activation or not, does not induce protection (26). Rabbit Polyclonal to ACOT2. Considering the immunodominant cellular immune response elicited by gp43, which may halt the progression of the infection, along with a locally effective but generally unprotective antibody response to this antigen, we aimed in the present investigation at determining the epitopes mediating these immune responses and at selecting those that could MP470 be used in vaccination trials. We show here that a 15-amino-acid peptide (P10) contained in gp43 is responsible for glycoprotein-mediated T-cell activation and protection against PCM in BALB/c mice without eliciting antibodies directed against the native antigen. MATERIALS AND METHODS Purification of gp43 from B-339, originally obtained from A. ...
Plabr 2734 - Riboflavin biosynthesis protein - Rubinisphaera brasiliensis (strain ATCC 49424 / DSM 5305 / JCM 21570 / NBRC...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Bioline International Official Site (site up-dated regularly)
in human serum samples of patients with paracoccidioidomycosis using the polymerase chain reaction (PCR). The diagnosis of paracoccidioidomycosis (PCM) was defined by microscopic observation of the fungus on direct exam or histopathology, culture, and serological positivity. DNA from serum of 33 patients with PCM was extracted and submitted to nested-PCR using primers from the gp 43 gene. Only one sample was positive on nested-PCR. We conclude that the prevalence of fungemia in patients with different clinical forms of PCM is low, limiting the use of serum DNA detection as an alternative diagnostic tool ...
ICD-10 Diagnosis Code B41.7 Disseminated paracoccidioidomycosis
Diagnosis Code B41.7 information, including descriptions, synonyms, code edits, diagnostic related groups, ICD-9 conversion and references to the diseases index.
VTT D-01672 Strain Passport - StrainInfo
Aspergillus brasiliensis strain CBS 733.88 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial ...
ReP USP - Resultado da busca
SHIKANAI YASUDA, Maria Aparecida; PEREIRA, P M; YAMASHIRO KANASHIRO, Edite; et al. Lung tissue mechanics in the early stages of induced paracoccidioidomycosis in rats. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 30, n. 10, p. 1175-1179, 1997. Disponível em: < http://www.scielo.br/cgi-bin/fbpe/fbtext?got=last&pid=S0100-879X(97)03001006&usr=fbpe&lng=en&seq=0100-879X-010&nrm=iso&sss=1&aut=71981947 > DOI: 10.1590/s0100-879x1997001000006 ...
Aid to a Declining Matriarch in the Giant Otter Pteronura brasiliensis - pdf descargar
Aid to a Declining Matriarch in the Giant Otter Pteronura brasiliensis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Actinoplanes brasiliensis
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinoplanes brasiliensis.
PCM Fund, PCM:NYQ summary - FT.com
Latest PCM Fund (PCM:NYQ) share price with interactive charts, historical prices, comparative analysis, forecasts, business profile and more.
paracoccidioidomycosis(South American Blastomycosis, Brazilian Blastomycosis, Lutz-Splendore-Almeida disease, Mucocutaneous...
Azevedo, RS, Gouvêa, AF, Lopes, MA, Corrêa, MB, Jorge, J. Synchronous oral paracoccidioidomycosis and oral squamous cell carcinoma with submandibular enlargement. Med Mycol. . vol. 49. 2011. pp. 84-9. (Describes similarities and differences between the oral lesions of paracoccidioidomycosis and oral squamous cell carcinomas. Emphasizes and discusses the significance of risk factors in the association of these two entities.). de Freitas, RM, Prado, R, do Prado, FL, de Paula, IB, Figueiredo, MT, Ferreira, CS, Goulart, EM, Pedroso, ER. Pulmonary paracoddidioidomycosis: radiology and clinical-epidemiological evaluation. Rev Soc Bras Med Trop . vol. 43. 2010. pp. 651-656. (Compares respiratory signs and symptoms between patients with and without chest X-ray abnormalities in order to establish the meaning of radiographic findings in pulmonary paracoccidioidomycosis.). Goldani, LZ. Gastrointestinal paracoccidioidomycosis: an overview. J Clin Gastroenterol. vol. 45. 2010. pp. 87-91. (Review ...
Pseudotumoral neuroparacoccidioidomycosis of the posterior fossa: A case report and review of the literature - Surgical...
1. Almeida SM, Queiroz-Telles F, Teive HAG, Ribeiro CEL, Werneck LC. Central nervous system paracoccidioidomycosis: Clinical features and laboratorial findings. J Infect. 2004. 48: 193-8. 2. Cunha MLV, Castro CAO, Piekala C, Neto JFA, Pletz ALB. Neuroparacoccidioidomycosis simulating cerebral metastasis: Case report and literature review. J Bras Neurocirurg. 2012. 23: 226-33. 3. Elias J, dos Santos AC, Carlotti CG, Colli BO, Canheu A, Matias C. Central nervous system paracoccidioidomycosis: Diagnosis and treatment. Surg Neurol. 2005. 63: 13-21. 4. Faria AV, Dabus GC, Zanardi VA, Cendes F. Proton magnetic resonance spectroscopy and magnetic resonance imaging findings in a patient with central nervous system paracoccidioidomycosis. J Neuroimaging. 2004. 14: 377-9. 5. Lambertucci JR, Lana-Peixoto MA, Pitella JEH. Paracoccidioidomycosis of the central nervous system. Rev Soc Bras Med Trop. 2001. 34: 395-6. 6. Magalhaes AC, Caramelli P, Silva ED, Bacheschi LA, Lo LS, Menezes JR. Magnetic resonance ...
Bioline International Official Site (site up-dated regularly)
Paracoccidioidomycosis should be differentiated from other opportunistic diseases in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients who live in Latin America. Laboratory investigation can begin with serological tests, which are rapid and efficient. In the present study, double immunodiffusion (DID), counterimmunoelectrophoresis (CIEP) and an enzyme linked immunosorbent assay (ELISA) tests were assessed for the detection of anti-Paracoccidioides brasiliensis antibodies in 40 patients coinfected with HIV. The results were compared to those obtained for 75 non-HIV-infected patients with endemic paracoccidioidomycosis. Anti-P. brasiliensis antibodies were detected in 65% (DID), 79% (CIEP) and 95% (ELISA) of the patients with HIV/AIDS, significantly lower rates than those detected in cases of endemic paracoccidioidomycosis, which were 89%, 99% and 100%, respectively. The reactive sera of HIV-infected patients also showed lower anti-P. brasiliensis antibody ...
Scopulariopsis chartarum Systemic Mycosis in a Dog | Journal of Clinical Microbiology
A 2-year-old, mixed-breed, male dog that had been captured by animal control officials was presented to the Oklahoma Animal Disease Diagnostic Laboratory for euthanasia and diagnostic tests. The dog was initially found to be dehydrated, and the animal had diarrhea over the first 10 days of laboratory confinement. The course worsened to episodes of myoclonia and convulsions. The dog displayed chewing gum fits and other nervous disorders and ultimately advanced to a comatose state which caused the kennel personnel to request that the animal be euthanized. The clinical diagnosis was infectious canine distemper.. At necropsy, the predominant lesions discovered were enlarged kidneys with a purulent exudate in the renal pelvis and scattered, 3- to 4-mm yellow foci in the renal parenchyma and raised 1- to 3-cm splenic plaques and white foci in the interventricular septum of the heart. The meninges had 2- to 3-mm yellow foci, and there was an area of liquefaction necrosis in the rostral area of the ...
Paracoccidioidomycosis - Wikipedia
Primary infection, although poorly understood due to lack of data, is thought to occur through inhalation of the conidia through the respiratory tract, after inhaling fungal conidia produced by the mycelial form of P. brasiliensis.[10][16] This occurs predominantly in childhood and young adulthood, after exposure to agricultural activity.[9] Infection may occur through direct skin inoculation, although this is rare.[11]. After inhalation into the alveoli, there is rapid multiplication of the organism in the lung tissue, sometimes spreading via the venous and lymphatic systems.[10] Approximately 2% of people develop clinical features after the initial asymptomatic infection.[11]. The type of immune response determines the clinical manifestation of the infection, with children and HIV co-infected individuals most commonly developing the acute/subacute disseminated disease.[10] Most of those infected develop a Type 1 T-cell (Th1) mediated immune response, resulting in alveolitis and compact ...
2020 ICD-10-CM Diagnosis Code B41.8: Other forms of paracoccidioidomycosis
Free, official coding info for 2020 ICD-10-CM B41.8 - includes detailed rules, notes, synonyms, ICD-9-CM conversion, index and annotation crosswalks, DRG grouping and more.
Parasite of the Day: Urogasilus brasiliensis
Todays featured parasite, Urogasilus brasiliensis, is a newly described species that has been found in some freshwater fish living in the Cristalino River, a tributary of the Araguaia River in Brazil. The known hosts to this parasite include the tiger fish and two species of peacock bass. Much like other parasites that infect many different species of hosts, some hosts are just better than others and that is the case for U. brasiliensis too. This copepod tends be more common in the tiger fish and grows to a larger size in that host, indicating that it is possibly a better host for the parasite than the peacock bass. But while U. brasilensis is not particularly picky about what species of fish it infects, it is picky about where it lives within that fish - it is always found in the bladder ...
Research Highlights: The History of New Dimorphic Fungal Pathogen: Emergomyces - Chromoscience
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Viral Infection
The inflammation of the mucous membranes of the bronchial tubes is called bronchitis. It occurs in both acute and chronic forms. If occurring in an athlete, bronchitis is more likely to be in acute form. Acute bronchitis usually occurs as … [Read more...] ...
Diarrhea | RxHealthMed
Diarrhea is a symptom, not a disease. It is defined as passing frequent (3 or more per day) loose or watery stools. The acute form lasts less than 14 days (usually only a few days), goes away on its own, and usually isnt serious, but it can be linked with…