Changes in free intracellular Ca2+ concentration regulate insulin secretion from pancreatic β-cells. The existence of steep Ca2+ gradients within the β-cell requires the presence of specialized Ca2+ exclusion systems. In this study we have characterized the plasma membrane Ca2+-ATPases (PMCAs) which extrude Ca2+ from the cytoplasm. PMCA isoform- and subtype-specific mRNA expression was investigated in rodent pancreatic α- and β-cell lines, and in human and rat islets of Langerhans using reverse-transcription PCR with primers flanking the calmodulin-binding region of rat PMCA. The expression pattern of PMCA 1 and 2 was conserved in different species and islet-cell types since both rat and human islets of Langerhans and all cell lines tested contained the 1b and 2b forms. PMCA 4 isoform subtypes, however, were expressed in a cell-type-specific manner since β-cells expressed PMCA 4b only, whereas in islets of Langerhans, which contain α, β, δ and polypeptide-secreting cells, PMCA 4a and 4b ...
Ghrelin is a hormone produced mainly by P/D1 cells lining the fundus of the human stomach and epsilon cells of the pancreas that stimulates hunger
We used mice lacking Abcc8, a key component of the β-cell KATP-channel, to analyze the effects of a sustained elevation in the intracellular Ca2+ concentration ([Ca2+]i) on β-cell identity and gene expression. Lineage tracing analysis revealed the conversion of β-cells lacking Abcc8 into PP-cells, but not to α- or δ-cells. RNA-Seq analysis of FACS-purified Abcc8-/- β-cells confirmed an increase in Ppy gene expression, and revealed altered expression of over 4,200 genes, many of which are involved in Ca2+-signaling, the maintenance of β-cell identity, and cell adhesion. The expression of S100a6 and S100a4, two highly up-regulated genes, is closely correlated with membrane depolarization, suggesting their use as markers for an increase in [Ca2+]i. Moreover, a bioinformatics analysis predicts that many of the dysregulated genes are regulated by common transcription factors, one of which, Ascl1, was confirmed to be directly controlled by Ca2+ influx in β-cells. Interestingly, among the ...
But the use of the term is far from settled in public. For example, the Minneapolis Star-Tribune wrote a story on September 27th characterizing the new device as an artificial pancreas. The next day it published an article that considered the new MiniMed pump and system as a step towards an artificial pancreas.. To be clear, there is no system available now, or in the pipeline, that fully replicates the functions of the pancreas in any person, with or without diabetes. To do so would require a device to govern a complex relationship between beta (insulin), alpha (glucagon), delta (somatostatin), and gamma (pancreatic polypeptide) cells. The interaction between these cells and the nervous system is not even fully understood. If thats what an artificial pancreas must be, then the MiniMed isnt it.. Others, like Medtronic representatives, characterize a device like the MiniMed as an artificial pancreas because it automates some pancreatic functions, much like the body does. The biggest value ...
The pancreas is a glandular organ in the upper abdomen that serves as both a hormone-producing endocrine gland and a digestive exocrine gland. The endocrine cells are located in the islets of Langerhans, which are irregularly shaped patches of tissue that release hormones directly into the bloodstream (Islets of Langerhans). There are five known types of hormone-producing cells in the pancreas: alpha cells producing glucagon, beta cells producing insulin, delta cells producing somatostatin, PP cells producing pancreatic polypeptide, and epsilon cells producing ghrelin.. All of these hormones work together to maintain a balance in the body. Insulins main function is lowering glucose concentration in the bloodstream, and glucose works to raises sugar levels when they are too low. Somatostatin inhibits glucagon and insulin secretion, PP regulates pancreatic secretion activities and ghrelin regulates appetite, while also playing a role in regulating energy use. Endocrine features of PWS include low ...
We reported a significant increase of ghrelin epsilon-cells in the pancreas and basal serum after Sleeve gastrectomy versus the control groups. The epsilon cellular increment was related to neogenesis, as the neurogenin-3 marker revealed. The Roux-en Y Gastric Bypass showed neither epsilon cell increase nor basal serum changes in ghrelin release. As a conclusion, we reported that the severe suppre...
NeuroD1兔多克隆抗体(ab76685)可与人样本反应并经WB, IHC, ICC/IF实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
NeuroD1兔多克隆抗体(ab16508)可与小鼠, 人, 小吻翎电鳗样本反应并经WB, IHC, ICC, ICC/IF实验严格验证,被7篇文献引用并得到8个独立的用户反馈。
Pancreatic Polypeptide product information; Pancreatic Polypeptide is available 9 times from supplier ChemScene at Gentaur.com shop
This test measures a substance in your blood called pancreatic polypeptide. Higher levels may mean you have a type of pancreatic tumor.