Low values of serum elastase activity and high values of serum elastase inhibitors were strongly and independently associated with increased carotid plaque occurrence in this 4-year longitudinal study performed in a large sample of relatively aged subjects. The magnitude of the associations and the consistency of the results are noteworthy, and this is the first investigation that reports the relations between serum elastase parameters and subsequent development of atherosclerosis.. In our study, no association was observed between serum elastase activity and serum elastase inhibitors. The elastase-type activity measured in this study was derived, up to 90%, from metallo-endopeptidases5 such as MMP-2 and MMP-9.15 Their specific inhibitors, TIMPs (tissue inhibitors of metalloproteinases),16 were not measured. The major source of the elastase inhibitors determined in this investigation was α1-proteinase inhibitor and, to a lesser extent, α2-macroglobulin.5 α1-Proteinase inhibitor has been shown ...
Pancreatic elastase is a form of elastase that is produced in the acinar cells of the pancreas, initially produced as an inactive zymogen and later activated in the duodenum by trypsin. Elastases form a subfamily of serine proteases, characterized by a distinctive structure consisting of two beta barrel domains converging at the active site that hydrolyze amides and esters amongst many proteins in addition to elastin, a type of connective tissue that holds organs together. Pancreatic elastase 1 is a serine endopeptidase, a specific type of protease that has the amino acid serine at its active site. Although the recommended name is pancreatic elastase, it can also be referred to as elastase-1, pancreatopeptidase, PE, or serine elastase. The first isozyme, pancreatic elastase 1, was initially thought to be expressed in the pancreas. However it was later discovered that it was the only chymotrypsin-like elastase that was not expressed in the pancreas. In fact, pancreatic elastase is expressed in ...
An elastase-induced emphysema model was utilized to determine if hamsters with preexisting lung disease were more susceptible to lung damage from air-pollutant exposure. Male golden hamsters, divided into two treatment groups, were given a single intratracheal injection of either 6 units of porcine pancreatic elastase (EMP) or buffer (CNT). After a 4-week recovery period, equal numbers of each group were exposed 23 hr/day x 28 day to filtered air (AIR) or to the complex by-products from a dark-phase-reaction mixture of trans-2-butene, ozone, and sulfur dioxide (MIX). Lung-function measurements on the elastase-treated groups showed changes consistent with mild emphysema. There were no significant differences in lung volumes or lung compliance between the AIR- and MIX-exposed animals. However, the nitrogen washout slope decreased and the diffusing capacity for carbon monoxide increased in both the CNT and EMP hamsters exposed to the MIX. The change in diffusing capacity was greater in normal ...
TY - JOUR. T1 - Elastase-induced saccular aneurysms in rabbits. T2 - A dose-escalation study. AU - Kallmes, David F.. AU - Fujiwara, Naomi H.. AU - Berr, Stuart S.. AU - Helm, Gregory A.. AU - Cloft, Harry J.. PY - 2002/3/2. Y1 - 2002/3/2. N2 - Background and purpose: Reproducible animal models facilitate preclinical assessment of aneurysm therapies. Our purpose was to determine if increased elastase doses enlarge aneurysms and parent arteries. Methods: Rabbit right common carotid artery (CCA) aneurysms were created with distal ligation and intraluminal elastase incubation. Groups were 1) sham (no elastase, n = 3), 2) 25% elastase (10 minutes, n = 9), 3) 50% elastase (10 minutes, n = 7), and 4) 50% elastase (20 minutes, n = 41). Angiography was performed after 14 days. Resultant aneurysm width and height and parent artery diameters were measured and compared with the Student t or Mann-Whitney (Wilcoxon rank sum) test. Results: Proximal segments were enlarged in all elastase subjects and no sham ...
The elastase structural gene (lasB) from Pseudomonas aeruginosa PAO1 has been previously cloned on an 8-kilobase (kb) DNA fragment. The lasB gene, cloned in both orientations in pUC18, produced elastase in Escherichia coli, indicating that its promoter and translation initiation sites are functional in E. coli. Deletion analysis further defined the location of the lasB gene to a 3.0-kb EcoRI-KpnI fragment (pRB1803). Elastase prepared from E. coli TB1 (pRB1803) corresponded in molecular weight to mature P. aeruginosa extracellular elastase (33,000). The lasB gene directed the synthesis of 54- and 50-kilodalton (kDa) proteins in a bacterial cell-free transcription-translation system. The 33-, 50-, and 54-kDa proteins reacted with elastase-specific antiserum. To further characterize the lasB gene, the nucleotide sequence of the 3.0-kb EcoRI-KpnI fragment was determined. This DNA fragment contained a 1,491-base-pair open reading frame encoding 498 amino acids, corresponding to a predicted molecular ...
1E38: PORCINE PANCREATIC ELASTASE COMPLEXED WITH (3S, 4S)N-PARA-NITROBENZENESULPHONYL -3-ETHYL-4-(CARBOXYLIC ACID)PYRROLIDIN-2-ONE SOAKED IN PH 9 BUFFER FOR 2 MINUTES
Chymotrypsin-like elastase family member 1 (CELA1) also known as elastase-1 (ELA1) is an enzyme that in humans is encoded by the CELA1 gene. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Elastase-1 was formerly designated pancreatic elastase 1. However unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. Hence this enzyme has been renamed as elastase-1. To date, elastase 1 expression has only been detected in skin keratinocytes. Literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B CELA3B). This enzyme has been linked to chronic pancreatitis . GRCh38: Ensembl release 89: ENSG00000139610 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000023031 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse ...
Endovascular coiling is an acceptable treatment of intracranial aneurysms, yet long term follow-ups suggest that endovascular coiling fails to achieve complete aneurysm occlusions particularly in wide-neck and giant aneurysms. Placing of a stentlike device across the aneurysm neck may be sufficient …
Generation of a Pseudomonas aeruginosa elastase gene targeted deletion mutant by Red recombination system%采用Red重组系统敲除铜绿假单胞菌弹性蛋白酶基因. Institute of Scientific and Technical Information of China (English). 余华; 熊浚智; 何晓梅; 盛哈蕾; 蔡文强; 谢玮; 张克斌. 2013-01-01. The aim of this study is to obtain the elastase activity negative strain by knocking out the elastase gene in Pseudomonas aeruginosa PAO1. Three genes of Red recombination system from λ phage were amplified and cloned into Esche-richia-Pseudomonas shuttle vector pUCP, and the pUCP-Red vector was transformed into PAO1 competent cells by electropo-ration. Then the recombinant DNA fragment which contains gentamycin antibiotic cassette flanked by two 80-bp homology sequences of elastase gene upstream and downstream locuses respectively was obtained by conventional cloning methods. And the fragment was electroporated into PAOl/pUCP-Red competent cells and screened on LB plate ...
TY - JOUR. T1 - Characteristic behavior of serum elastase 1 in pancreatic cancer.. AU - Kimura, T.. AU - Ito, T.. AU - Sumii, T.. AU - Muranaka, T.. PY - 1991/6. Y1 - 1991/6. N2 - Diagnostic significance of serum immunoreactive elastase 1 (IRE) in pancreatic cancer was evaluated in 53 patients with pancreatic cancer. Frequency of abnormally high serum IRE levels in pancreatic cancer was 66.0%; 87.0% in head cancer (N = 23), 55.0% in body & tail cancer (N = 20) and 40% in diffuse cancer (N = 10). Serum IRE level in resectable cancer was significantly higher than that in unresectable cancer. Comparative studies of serum pancreatic enzymes revealed that serum IRE was the most sensitive marker for diagnosis of pancreatic cancer. The characteristic behavior of serum IRE throughout the course of pancreatic cancer was that abnormally high levels of IRE are maintained for a longer period of time when compared to that of pancreatitis. In the comparative study of serum IRE and CA19-9, of the 9 cases of ...
Addresses: OXFORD CTR MOL SCI, OXFORD OX1 3QY, ENGLAND. DYSON PERRINS LAB, OXFORD OX1 3QY, ENGLAND. UNIV OXFORD, MOL BIOPHYS LAB, OXFORD OX1 3QU, ENGLAND. UNIV UPPSALA, DEPT BIOCHEM, S-75123 UPPSALA, SWEDEN.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-15 ...
Neutrophil elastase (NE) suppresses IL-8/CXCL8 in human airway smooth muscle cells (hASM) while stimulating its production in respiratory epithelial cells. This differential effect is mediated by the selective induction of NKRF and dysregulation in chronic inflammatory diseases. We hypothesized that the differential activation of NF-κB subunits confer the opposite effect of NKRF on IL-8/CXCL8 in primary hASM and A549 cells stimulated with NE. The events occurring at the promoters of NKRF and IL-8/CXCL8 were observed by ChIP assays, and the functional role of RelB was confirmed by knockdown and overexpression. Although p65 was stimulated in both cell types, RelB was only activated in NE-treated hASM, as confirmed by NF-κB DNA binding ELISA, Western blotting and confocal microscopy. Knockdown of RelB abolished the induction of NKRF and converted the suppression of IL-8/CXCL8 to stimulation. The forced expression of RelB induced NKRF production in hASM and A549 cells. NE activated the NIK/IKK1/RelB non
The transformation from carcinoma in situ to invasive carcinoma occurs when tumor cells traverse extracellular matracies allowing them to move into parenchymal tissues. Tumor invasion may be aided by the secretion of collagen and elastin degrading proteases from tumor and tumor-associated cells. In this study ~ the production of Type I and Type V collagen degrading activities and elastolytic activities by DLD-1 human colon carcinoma cells, B16-F10 murine melanoma cells, and normal human dermal fibroblasts was examined. DLD-1 cells and normal fibroblasts produced similarly high levels of collagenolytic activity. DLD-1 cells also produced high levels of elastinolytic activity; this activity was found exclusively in the extracellular medium. DLD-1 cells and normal fibroblasts produced more collagen and elastin degrading activity than did B16-F10 melanoma cells, a cell line characterized as highly metastatic. The Type I and Type V collagenolytic activities from DLD-1 cells were separated and characterized
|p|ZD-0892 is a selective and potent inhibitor of a neutrophil elastase with Kis of 6.7 and 200 nM for human neutrophil elastase and porcine pancreatic elastase, respectively.|/p||p||/p||p|ZD0892 administration to DBA/2 mice infected with the EMC virus re
SSR 69071 is a high affinity, potent inhibitor of human leukocyte elastase (HLE) (IC50 = 3.9 nM), which displays species-selectivity (Ki values are 0.017, 1.70, 3.01, 58 and , 100 nM for human, mouse, rat, rabbit and porcine elastase respectively) and inhibits HLE-induced lung hemorrhage in mice (ID50 = 2.8 mg/kg) and reduces infarct size in an in vivo acute model of coronary ischemia-reperfusion injury. Orally active. Learn More ...
Enzyme Explorer Product Application Index for Elastase at Sigma-Adlrich online. Leukocyte elastase is a 29KDa serine endoprotease of the Proteinase S1 Family. It exists as a single 238 amino acid-peptide chain with four disulfide bonds.
... elastase 1, pancreatic Identifiers Symbol ELA1 Entrez 1990 HUGO 3308 OMIM 130120 RefSeq NM_001971 UniProt Q9UNI1 Other data EC number
To study specific aspects of human respiratory diseases and its treatment it is necessary to measure the induced symptoms and the impairment of lung function in living animals. For example, key features of asthmatic inflammation (airway hyperresponsiveness, eosinophilic inflammation together with an increase in activated T cells in the airways) are induced in actively sensitized Brown Norway (BN) rats exposed to allergen (ovalbumin, OVA). Alternatively, an inflammation similar to that observed in chronic obstructive pulmonary disease (COPD) patients (neutrophilia and mucus cell metaplasia) can be elicited in rodents by the administration of endotoxin (lipopolysaccharide; LPS). Emphysema, one of the most critical components of COPD, which results in the destruction of lung parenchymal tissue by a number of proteases (neutrophil elastase or matrix metalloproteinases), is induced in rats by the application of a single dose of porcine pancreatic elastase. In all cases the symptoms resemble those ...
CELA2B Full-Length MS Protein Standard (NP_056933), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2B is secreted from the pancreas as a zymogen. In other species, elastase 2B has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues.
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A chronic obstructive pulmonary disease in which there is a loss of elasticity in the lung tissue. It may be a result of excessive leucocyte elastase activity because α1-antiprotease, which would normally inhibit the enzyme, is inactivated by reactive oxygen species produced in inflammation. ...
In the title complex, [CdCl2(C3H4N2S)4],the CdII atom has an (2006 ?); Davarski (1996 ?); Mac pc?ek & Davarski (1993 ?); Maniukiewicz (2004 ?); Raper (1981 ?); Suh (2005 ?, 2007 ?, 2009 ?). ??3 Data collection: (Bruker, 2001 ?); cell refinement: (Bruker, 2001 ?); data reduction: (Sheldrick, 2008 ?); system(s) used to refine structure: (Sheldrick, 2008 ?); molecular graphics: (Sheldrick, 2008 ?); software Elastase Inhibitor, SPCK supplier used to prepare Elastase Inhibitor, SPCK supplier material for publication: positions. The amino organizations participate in intra- and inter-molecular NHN and NHCl hydrogen bonds (Table 1). In the crystal structure molecules Elastase Inhibitor, SPCK supplier are interconnected by these relationships into a three-dimensional hydrogen relationship network (Fig. 2). Experimental A waterCethanol (1:1) remedy (40 ml) of 2-aminothiazole (5 mmol) was added dropwise to a waterCethanol (1:1) remedy (40 ml) of CdCl2.2.5H2O (2 mmol) with stirring. The small amount of ...
1.Gertler A, Hofmann T The involvement of the amino-terminal amino acid in the activity of pancreatic proteases. I. The effects of nitrous acid on elastase, J Biol Chem, 1967 ...
[65 Pages Report] Check for Discount on elastase from human leukocytes Global Market and Forecast Research report by ChemReport. DescriptionWe provide independent and unbiased information on manufacturers, prices, production...
Elastase molecule. Computer model of elastase, an enzyme produced in the pancreas that catalyses the hydrolysis of elastin. It is a compact globular protein consisting of a single polypeptide chain of 240 amino acids, which are cross-linked by 4 disulphide bridges. - Stock Image A605/0147
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Jochum, Marianne; Pabst, W. und Schill, W.-B. (1986): Granulocyte elastase as a sensitive diagnostic parameter of silent male genital tract inflammation. In: Andrologia, Vol. 18: S. 413-419 [PDF, 922kB] ...
S corresponding to hypermethylation in tumors (fold change ranged from 322670); in addition to another 10 genes showed more than 2 fold hypermethylation in
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View mouse Cela2a Chr4:141814954-141826005 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
homology in their active sites and have similar catalytic mechanisms [15,16]. Moreover, both coagulation factors and leukocyte elastase are inhibited by common inhibitor classes, including isocoumarins [17] and bikunin [9,11]. Therefore, we speculated that we could design a novel derivative of the second domain of bikunin that inhibits multiple enzymes, such as coagulation factors and leukocyte elastase, based on the structural homology of their active sites. In the present study, we tried to potentiate the leukocyte elastase inhibitory activity of the second domain of bikunin by amino acid substitution without losing anticoagulant activity. To search amino acid residues to be modified, we examined a theoretical model of the second domain of bikunin docked to the X-ray structure of human leukocyte elastase. Using this information, we generated a novel triple mutant that inhibits factor XIa, factor Xa, and leukocyte elastase. In addition, we constructed models of this novel variant as a complex ...
[55 Pages Report] Check for Discount on Neutrophil Elastase (Bone Marrow Serine Protease or Elastase 2 or Medullasin or PMN Elastase or Human Leukocyte Elastase or ELANE or EC 3.4.21.37) - Pipeline Review, H2 2017 report by Global Markets Direct. Neutrophil Elastase (Bone Marrow Serine Protease or Elastase 2 or...
Pseudomonas aeruginosa causes aggressive infection in patients with pre-existing disorders and recurrent pulmonary infections in cystic fibrosis patients. Pathogenesis of P. aeruginosa infections is multifactorial owing to numerous virulence factors. The focus of this thesis research was to investigate whether P. aeruginosa elastase (PE) causes remodeling of the cytoskeleton by increasing the phosphorylation of RhoA GTPase proteins. In addressing our hypothesis, we utilized Small GTPase Immuno-sorbent Activation assays (G-LISA) and Enzyme linked Immuno-sorbent assay (ELISA) to quantitate changes in the total as well as phosphorylated RhoA protein in Calu3 cell lines. Fluorescence microscopy aided in understanding the changes in morphological organization of F-actin. Changes in expression of TJ protein, ZO1, due to PE induced RhoA GTPase activity, was analyzed with SDS PAGE and Western Blot Analysis. Our data from G-LISA and ELISA assays indicate that PE increases the amount of active RhoA protein by 50
Abstract: 53 patients with lung tuberculosis were divided in 3 groups in accordance with severety of disease. Leukocyte elastase, cationic proteins in neutrophils, activities of a 1-proteinase and a 2-macroglobulin were determined in patients plasma. Thromboelastographic, coagulating, fibrinolytic indices, and antithrombin III activity were also determined in 28 patients of all 3 groups. Results demonstrated the high level of leukocyte elastase (6-fold more than normal) in plasma of patients with acute tuberculosis process. This group of patients demonstrated activation of intravascular coagulation proceeded on the background of significant decrease (up to 60%) of AT III activity. Conclusion: Acuity and severety of tuberculosis process in lung may be characterized by high activity of leukocyte elastase. Degranulating activity of neutrophils and releasing of elastase are the reason of AT III deficiency and increasing of intravascular coagulating activity in tuberculosis ...
Pathobiochemical Significance of Granulocyte Elastase Complexed with Proteinase Inhibitors: Effect on Glycosaminoglycan Metabolism in Cultured Synovial ...
Pseudomonas aeruginosa PAO mutants defective in elastase were isolated by plate assays of nitrosoguanidine-mutagenized clones. A total of 75 elastase mutants were isolated from 43,000 mutagenized clones. One mutant (PAO-E64) was apparently identical to the parental strain except for its deficiency in elastase activity. This mutant produced an enzyme which was antigenically indistinguishable from parental elastase. Furthermore, equal levels of elastase antigen were produced by this mutant and its parental strain. The mutant elastase, however, had greatly reduced enzymatic activity. Mutant PAO-E64 is presumed to have a mutation in the structural gene for elastase. We have designated the genotype of the mutation in PAO-E64 as lasA1. ...
A subset of osteoarthritis (OA) patients experience joint pain with neuropathic characteristics. Mediators such as neutrophil elastase, a serine proteinase, may be released during acute OA inflammatory flares. We have previously shown that local administration of neutrophil elastase causes joint inflammation and pain via activation of proteinase-activated receptor-2 (PAR2). The aim of this study was to examine the contribution of endogenous neutrophil elastase and PAR2 to the development of joint inflammation, pain, and neuropathy associated with monoiodoacetate (MIA)-induced experimental OA. MIA (0.3 mg/10 μl) was injected into the right knee joint of male C57BL/6 mice (20-34 g). Joint inflammation (edema, leukocyte kinetics), neutrophil elastase proteolytic activity, tactile allodynia, and saphenous nerve demyelination were assessed over 14 days post-injection. The effects of inhibiting neutrophil elastase during the early inflammatory phase of MIA (days 0 to 3) were determined using sivelestat (50
TY - JOUR. T1 - Neutrophil elastase induces IL-8 synthesis by lung epithelial cells via the mitogen-activated protein kinase pathway. AU - Chen, Hao Cheng. AU - Lin, Horng Chyuan. AU - Liu, Chien Ying. AU - Wang, Chun Hua. AU - Hwang, Tritium. AU - Huang, Tzu Ting. AU - Lin, Chien-Huang. AU - Kuo, Han Pin. PY - 2004. Y1 - 2004. N2 - The sequestration of neutrophils in the lung and the release of proinflammatory mediators, including neutrophil elastase, are responsible for sepsis-induced microvascular permeability and alveolar epithelial cell damage. To assess the underlying mechanism, human neutrophil elastase (0.01-0.5 μg/ml) was added to cultured A549 epithelial cells in the presence or absence of inhibitors. IL-8 was analyzed by ELISA or by RT-PCR to measure the IL-8 synthesis capacity. Mitogen-activated protein kinase (MAPK) activity was detected by Western blot analysis. Neutrophil elastase dose-dependently increased IL-8 release from cultured A549 epithelial cells. Pretreatment with a ...
BioAssay record AID 99580 submitted by ChEMBL: Human leukocyte elastase (HLE) inhibitory activity is reported as kinetic constant (kobs/[I] - time dependent inhibition).
RS)-Diethyl-2-benzyl-succinate was resolved using alpha-chymotrypsin. The two enantiomers were then elaborated to yield (S)-(+) and (R)-(-)-3-benzyl-N-[(methyl-sulfonyl)oxy]succinimide and the inhibitory activity of the two enantiomers toward human leukocyte elastase was subsequently determined. The k2/KI values for the R and S isomers were found to be 330 and 1500 M-1 s-1, respectively ...
Title: Neutrophil Elastase Inhibition: A New Cancer Therapy. VOLUME: 4 ISSUE: 2. Author(s):Takashi Sato, Miwa Yoshida, Satoshi Takahashi, Takashi Fukutomi and Jun-Ichi Yamashita. Affiliation:Department of Breast Oncology,Okazaki City Medical Association Public Health Center, Tatsumi-Nishi 1-9-1, Okazaki 444-0875, Japan.. Keywords:Neutrophil Elastase, Cancer Therapy, endothelial cells, metastatic organ, neutral serine protease, plasminogen activator (PA). Abstract: Cancer cells enter the circulation and attach to endothelial cells to pass through them and migrate over a distance to enter the tissue of the metastatic organ to proliferate there. In the same way, neutrophils drift in blood and adhere loosely to adhesive molecules on the endothelial cells in an inflamed area. They roll along the endothelial cells and then adhere closely to the endothelial cells to penetrate vessel wall. Neutrophils can destroy the basement membrane and migrate over a distance to fight against foreign bodies. Thus, ...
Secretion of Pseudomonas aeruginosa elastase, exotoxin A, and alkaline protease in sputum during bronchopulmonary exacerbations was examined in 18 cystic fibrosis patients chronically infected with this microorganism. The patients were studied during one or several exacerbation periods necessitating hospitalizations of 12 to 20 days. In all cases, P. aeruginosa was present in bronchial secretions at admission and was not eradicated after treatment. The P. aeruginosa density decreased significantly after antibiotic therapy but remained greater than 10(6) CFU/g of sputum in most cases. Significant amounts of P. aeruginosa exoproteins were measured in total homogenized bronchial secretions by immunoenzymatic assays. The detection of higher levels of exoproteins at admission, the significant decrease after treatment, and the absence of exoproteins during intercrisis phases constituted arguments for a renewal of virulence of P. aeruginosa during exacerbations. Nevertheless, the concomitant changes in ...
Fingerprint Dive into the research topics of Inhibitory effects of Bichalcone derivatives on Superoxide anion generation (O,sub,2,/sub, ,sup,•-,/sup,) and elastase release by activated human neutrophils in response to FMLP/CB. Together they form a unique fingerprint. ...
BioAssay record AID 67139 submitted by ChEMBL: Binding constant on human neutrophil elastase was determined at 20 percent and at 95 minutes for maximum inhibition and no enzyme activity was recovered over the 2 h-time.
Acquired elastase deficiency also can lead to increased hernia formation. In 1981, Cannon and Read found that the increased serum elastase and decreased alpha1 -antitrypsin levels associated with smok... more
The respiratory tract is normally kept essentially free of bacteria by cilia-mediated mucus transport, but in chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF), bacteria and mucus accumulates instead. To address the mechanisms behind the mucus accumulation, the proteome of bronchoalveolar lavages from COPD patients and mucus collected in an elastase-induced mouse model of COPD was analyzed, revealing similarities with each other and with the protein content in colonic mucus. Moreover, stratified laminated sheets of mucus were observed in airways from patients with CF and COPD and in elastase-exposed mice. On the other hand, the mucus accumulation in the elastase model was reduced in Muc5b-KO mice. While mucus plugs were removed from airways by washing with hypertonic saline in the elastase model, mucus remained adherent to epithelial cells. Bacteria were trapped on this mucus, whereas, in non-elastase-treated mice, bacteria were found on the epithelial cells. We propose that ...
Abcams Neutrophil Elastase ELISA Kit (ab119553) suitable for Cell culture supernatant, Serum, Plasma in human. Reliably quantify 1.98 pg/ml of Neutrophil…
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Introduction Prevalence of abdominal aortic aneurysm is 2-6%. Aneurysm is on the leading possitions in death cause. Lot of patients die do to ruptured aneurysm. The etiology of abdominal aortic aneurysm remains unknown. Current treatment(resection or endovascular)is not indicated for everyone. We don´t know the right conservative (pharmacological) treatment. Aims With our study, we would like to confirm the impact of atorvastatin on experimental abdominal aortic aneurysm. We would like to evaluate and quantified changes in the composition of an aneurysmatic wall under the effect of atorvastatin. Other aim is to make a summary of known knowledge of this disorder. These knowledges can help in searching for new treatment options. Methods Comparison of 2 groups of pigs with experimental abdominal aortic aneurysm. Group treated with atorvastatin and group without any pharmacological influencing. Comparing of aneurysmal growth rate. Evaluation of changes of the wall structure in both groups using a ...
Summary An elastase of Staphylococcus epidermidis was purified by ion exchange chromatography on CM-Sepharose and characterised. Its Mr is c. 21 kDa, its optimal temperature for activity is 42°C and the pH optimum is 6·8. The enzyme is activated by cysteine and other SH-donators and inhibited by l-trans-epoxy-succinylleucylamido-(4-guanidino)butane (E64), an inhibitor of cysteine proteases, but not by 3,4-dichloroisocoumarin (3,4-DCI), an inhibitor of serine proteases. This finding suggests that the elastase of S. epidermidis is a cysteine protease. Because S. epidermidis elastase degrades human sIgA, IgM, serum albumin, fibrinogen, and fibronectin, this enzyme may be regarded as a virulence factor.