TY - JOUR. T1 - Xanthine oxidase-mediated intracellular oxidative stress in response to cerulein in rat pancreatic acinar cells. AU - Suzuki, Hidekazu. AU - Suematsu, Makoto. AU - Miura, Soichiro. AU - Asako, Hiroshi. AU - Kurose, Iwao. AU - Ishii, Hiromasa. AU - Houzawa, Shigenari. AU - Tsuchiya, Masaharu. PY - 1993/7. Y1 - 1993/7. N2 - Intralobular oxygen radical formation was examined in cerulein-stimulated rat pancreatic acinar cells by digital imaging microscopic fluorography using a hy-droperoxide-sensitive fluorescent probe, dichlorofluores-cin (DCFH) diacetate. The isolated pancreatic acinar cells loaded with DCFH diacetate were microscopically observed, and the dichlorofluorescein (DCF) fluorescence yielded by DCFH oxidation via hydroperoxides was digitally processed. Within the initial 20 min after the application of cerulein (10 μM), intracellular oxidative stress was observed as indicated by the increase in DCF fluorescence intensity and reached its maximum at 60 min. DCF ...
Human Pancreatic Stem Cell Culture Extra-cellular Expansion Matrix is essential for Expansion of Human Pancreatic Stem Cell Cultures. This product requires Human Pancreatic Stem Cell Culture Media Cat#M36004-05 and Cells Cat# 36004-05. Also available Products ...
TY - JOUR. T1 - Transporter-mediated bile acid uptake causes Ca2+-dependent cell death in rat pancreatic acinar cells. AU - Kim, Joo Young. AU - Kim, Kyung Hwan. AU - Lee, Jin Ah. AU - Namkung, Wan. AU - Sun, An Qiang. AU - Ananthanarayanan, Meena. AU - Suchy, Frederick J.. AU - Shin, Dong Min. AU - Muallem, Shmuel. AU - Lee, Min Goo. PY - 2002. Y1 - 2002. N2 - Background & Aims: The mechanism by which cholelithiasis increases the risk of acute pancreatitis remains obscure. Because bile acids can enter the pancreas either by luminal diffusion or by interstitial leakage during gallstone impaction and pancreatitis is associated with impaired Ca2+ signaling, we examined the effect of bile acids on pancreatic acinar cell signaling and the associated intracellular events. Methods: Rat pancreatic acinar cells were isolated. by collagenase digestion and the effects of bile acids on [Ca2+]i signaling, cell survival, inflammatory signals, and the molecular and functional expressions of bile uptake ...
Agonist-specific cytosolic Ca2+ oscillation patterns can be observed in individual cells and these have been explained by the co-existence of separate oscillatory mechanisms. In pancreatic acinar cells activation of muscarinic receptors typically evokes sinusoidal oscillations whereas stimulation of cholecystokinin (CCK) receptors evokes transient oscillations consisting of Ca2+ waves with long intervals between them. We have monitored changes in the cytosolic Ca2+ concentration ([Ca2+]i) by measuring Ca2(+)-activated Cl- currents in single internally perfused mouse pancreatic acinar cells. With minimal intracellular Ca2+ buffering we found that low concentrations of both ACh (50 nM) and CCK (10 pM) evoked repetitive short-lasting Ca2+ spikes of the same duration and frequency, but the probability of a spike being followed by a longer and larger Ca2+ wave was low for ACh and high for CCK. The probability that the receptor-evoked shortlasting Ca2+ spikes would initiate more substantial Ca2+ waves was
TY - JOUR. T1 - New clinical aspects of pancreas divisum. AU - Tulassay, Z.. AU - Papp, J.. PY - 1980/1/1. Y1 - 1980/1/1. N2 - The clinical and pathogenetic features of pancreas divisum are surveyed on the basis of ERCP examination. This congenital anomaly of the pancreas can be distinguished from ductal obstruction on the basis of the radiographic configuration. In such patients the parenchyma of the pancreas can be properly evaluated only if the dorsal and ventral pancreatic ducts are visualized simultaneously. Pancreas divisum may be regarded as imposing an increased risk of supervening pancreatic diseases.. AB - The clinical and pathogenetic features of pancreas divisum are surveyed on the basis of ERCP examination. This congenital anomaly of the pancreas can be distinguished from ductal obstruction on the basis of the radiographic configuration. In such patients the parenchyma of the pancreas can be properly evaluated only if the dorsal and ventral pancreatic ducts are visualized ...
We have previously demonstrated [M. Campos-Toimil, T. Bagrij, J.M. Edwardson, P. Thomas, Two modes of secretion in pancreatic acinar cells: involvement of phosphatidylinositol 3-kinase and regulation by capacitative Ca2+ entry, Curr. Biol. 12 (2002) 211-215] that in rat pancreatic acinar cells, Gd3+-sensitive Ca2+ entry is instrumental in governing which second messenger pathways control secretory activity. However, in those studies, we were unable to demonstrate a significant increase in cytoplasmic [Ca2+] during agonist application as a result of this entry pathway. In the present study, we combined pharmacology with ratiometric imaging of fura-2 fluorescence to resolve this issue. We found that 2 μM Gd3+ significantly inhibits store-mediated Ca2+ entry. Furthermore, both the protonophore, CCCP (5 μM) and the mitochondrial Ca2+-uptake blocker, RU360 (10 μM), led to an enhancement of the plateau phase of the biphasic Ca2+ response induced by acetylcholine (1 μM). This enhancement was ...
Hedgehog (Hh) signaling regulates cell proliferation and differentiation in various organs during embryogenesis. In the pancreas, the role of this pathway is complex. At early stages, Hh signaling is excluded from the pancreas and ectopic activation of the pathway impairs pancreas formation by disturbing mesenchymal-epithelial interactions. In contrast to this inhibitory role, our preliminary data suggest a positive, cell autonomous role for Hh signaling during endocrine cell formation and function. The exact nature of this novel, cell autonomous activity remains to be elucidated. Little information is also available about the upstream mechanisms that regulate Hh signaling in pancreatic epithelium. Recent results have revealed that primary cilia, cellular appendages found on many cell types, control the level of Hh signaling activity. Primary cilia are present on adult duct and endocrine cells within the pancreas, the same cell types that are marked by expression of Ptc, a transcriptional target ...
Pancreatic Stem Cells Found in Adult Mice Thursday, 24 January 2008 Just as many scientists had given up the search, researchers have discovered that the pancreas does indeed harbour stem cells with the capacity to generate new insulin-producing beta cells. If the finding made in adult mice holds for humans, the newfound progenitor cells will represent an obvious target for therapeutic regeneration of beta cells in diabetes, the researchers report in the Jan. 25 issue of Cell, a publication of Cell Press. One of the most interesting characteristics of these [adult] progenitor cells is that they are almost indistinguishable from embryonic progenitors, said Harry Heimberg of the JDRF Center at Vrije Universiteit Brussel in Belgium and the JDRF Center for Beta Cell Therapy in Diabetes. In terms of their structure and gene expression, there are no major differences. They look and behave just like embryonic beta cell progenitors. Insulin is required for cells to take up blood sugar, the bodys ...
Accessory pancreas is a rare condition in which small groups of pancreatic cells are separate from the pancreas. They may occur in the mesentery of the small intestine, the wall of the duodenum, the upper part of the jejunum, or more rarely, in the wall of the stomach, ileum, gallbladder or spleen. The condition was first described by Klob in 1859. Accessory pancreas is a small cluster of pancreas cells detached from the pancreas and sometimes found in the wall of the stomach or intestines. After researching accessory pancreas at the University of Louisville medical library, I found a list of medical cases and the doctors that operated on the patients with this condition. In 1904, Dr. A. S. Warthin found 47 cases in the literature and added 2. Up to 1921, 31 cases were added. Twelve cases were found at operation. A number of patients have been operated upon for this trouble since then. E. J. Horgan found 2 cases in 321 consecutive autopsies. Locality of Accessory Pancreas, A. S. Warthin, 1904: ...
Description of disease Pancreas divisum. Treatment Pancreas divisum. Symptoms and causes Pancreas divisum Prophylaxis Pancreas divisum
Chronic pancreatitis is an inflammatory disease of the exocrine pancreas characterised by the replacement of pancreatic parenchyma by fibrosis.1 2 Apart from fibrosis, the pancreatic parenchyma may also exhibit enlarged ducts, infiltration by inflammatory cells, the formation of duct-like tubular complexes, regions of acinar cell degeneration, and duct cell proliferation.3 In human acute and chronic pancreatitis, pancreatic cancer, and animal models of acute pancreatitis, duct-like structures, referred to as tubular complexes, have been described.4 8 Tubular complexes evolve as the acinar cells decrease in height and lose acinar cell specific antigens, paralleled by increased expression of duct cell markers.9 39 The molecular alterations underlying the fibrotic changes and the formation of these duct-like complexes are largely unknown. Recently, tyrosine kinase receptors, comprising the large family of growth factor receptors, have been shown to be overexpressed in the pancreas of patients with ...
DNase I, Bovine Pancreas, |2000U/MG provided by the Genemart Bioscience Inc.,The Product Intro:DNase I, Bovine Pancreas, >2000U/MG
article{6854376, abstract = {Expansion of pancreatic beta cells in vivo or ex vivo, or generation of beta cells by differentiation from an embryonic or adult stem cell, can provide new expandable sources of beta cells to alleviate the donor scarcity in human islet transplantation as therapy for diabetes. Although recent advances have been made towards this aim, mechanisms that regulate beta cell expansion and differentiation from a stem/progenitor cell remain to be characterized. Here, we describe a protocol for an injury model in the adult mouse pancreas that can function as a tool to study mechanisms of tissue remodeling and beta cell proliferation and differentiation. Partial duct ligation (PDL) is an experimentally induced injury of the rodent pancreas involving surgical ligation of the main pancreatic duct resulting in an obstruction of drainage of exocrine products out of the tail region of the pancreas. The inflicted damage induces acinar atrophy, immune cell infiltration and severe ...
Transplants of insulin-secreting pancreas cells is a dream of medical science and medicos. A recent study shows that these grafts can actually allay some of the misery of patients with extreme diabetes.. Find the best Cyber Monday Deals 2017. They could actually prove to be a life-saving device. These transplants may get approval soon in the United States. The transplants are found in many other countries, yet they are only found in research studies in the United States. Starting from yesterday, the medical authorities have approved of the limited use of these transplants for diabetics who are in danger of seizures and death.. The sinking blood sugar levels are responsible for such a state of affairs. Most of these sufferers have Type 1 diabetes. The treatment of diabetes at the cellular level is an actuality and allays many of the nasty symptoms faced by patients.. In Type 1 diabetes, the immune system attacks the insulin-producing cells of the pancreas. Insulin is responsible for converting ...
Alfa Aesar™ Elastase, porcine pancreas 100mg Alfa Aesar™ Elastase, porcine pancreas Proteases and Protein-Cleaving Reagents
Hamster Pancreatic Epithelial Cells from Creative Bioarray are isolated from pancreatic tissue of pathogen-free laboratory mice. Hamster Pancreatic Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarrays Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended ...
C57BL/6 Mouse Primary Pancreatic Epithelial Cells from Creative Bioarray are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Pancreatic Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Creative Bioarrays Culture Complete Growth Medium for 3-7 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen ...
The Notch-signaling pathway is known to be fundamental in controlling pancreas differentiation. We now report on using Cre-based fate mapping to indelibly label pancreatic Notch-responsive cells (PNCs) at larval stages and follow their fate in the adult pancreas. We show that the PNCs represent a population of progenitors that can differentiate to multiple lineages, including adult ductal cells, centroacinar cells (CACs) and endocrine cells. These endocrine cells include the insulin-producing β-cells. CACs are a functional component of the exocrine pancreas; however, our fate-mapping results indicate that CACs are more closely related to endocrine cells by lineage as they share a common progenitor. The majority of the exocrine pancreas consists of the secretory acinar cells; however, we only detect a very limited contribution of PNCs to acinar cells. To explain this observation we re-examined early events in pancreas formation. The pancreatic anlage that gives rise to the exocrine pancreas is ...
TY - CHAP. T1 - Differentiation of pancreatic cells into hepatocytes. AU - Tosh, D. PY - 2006. Y1 - 2006. N2 - This chapter describes the use of the pancreatic cell line AR42J and mouse embryonic pancreas as models for the trans-differentiation of pancreas to liver. Both AR42J cells and embryonic pancreas can be induced to trans-differentiate to hepatocytes by exposure to the glucocorticoid dexamethasone. Dexamethasone can be replaced by the naturally occurring glucocorticoid cortisol to induce the conversion of AR42J cells to hepatocytes. To determine whether the effect of the glucocorticoid is specific, the cells can be exposed to RU486, the glucocorticoid receptor antagonist, prior to the addition of dexamethasone or cortisol. Because the embryonic pancreas contains both exocrine and endocrine cell types, it is possible, with the correct combination of antibodies, to immunostain for at least three cell types. AR42J cells can be obtained as a frozen aliquot or growing culture from the ECACC ...
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Liu, H.P.,Tay, S.S.,Leong, S.K. (1997). An ultrastructural study of the innervation of the guinea pig pancreas.. Journal für Hirnforschung 38 (1) : 107-117. ScholarBank@NUS Repository ...
Author(s): Gurlo, Tatyana; Butler, Peter C; Butler, Alexandra E | Abstract: Immunohistochemistry (IHC) and immunofluorescence (IF) staining techniques are important diagnostic tools of anatomic pathology in the clinical setting and widely used analytical tools in research laboratories. In diabetes research, they are routinely used for the assessment of beta- and alpha-cell mass, for assessment of endocrine cell distribution within the pancreas, for evaluation of islet composition and islet morphology. Here, we present the evaluation of IHC techniques for the detection of alpha-cells in human pancreatic tissue. We compared the Horse Radish Peroxidase (HRP)-based method utilizing DAB Peroxidase Substrate to the Alkaline Phosphatase (AP)-based method utilizing Vector Red substrate. We conclude that HRP-DAB staining is a robust and reliable method for detection of alpha-cells using either rabbit polyclonal or mouse monoclonal anti-glucagon antibodies. However, AP-Vector Red staining should be used with
Endocrinology. 2009 Feb;150(2):570-9. doi: 10.1210/en.2008-1009. Epub 2008 Oct 9. Evaluation Studies; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Govt
WEDNESDAY, Oct. 21, 2020 (HealthDay News) -- Scientists knew that dangerous T-cells lived in the pancreases of people with type 1 diabetes, but a new study shows they also take up residence in the pancreases of healthy individuals.. Researchers from the La Jolla Institute for Immunology in California used a new staining technique to show where these cells had gathered in human tissue samples. They were surprised that even tissue from healthy people showed these cells in high numbers in the pancreas.. Whats the difference? Even though healthy people have these immune system cells, people with type 1 diabetes have T-cells that are close to or infiltrate cell clusters inside the pancreas. Beta cells that live in these clusters make insulin to regulate blood sugar, but in people with type 1 diabetes the T-cells kill those beta cells.. These T-cells are like predators, said senior study author Dr. Matthias von Herrath, from the La Jolla Institute. And we always thought that beta cells would die ...
We show here that during pancreas development, blood vessels restrain pancreas tip cell formation and branching morphogenesis, and antagonize differentiation of epithelial cells into exocrine and endocrine fates. Consequently, the surprising net effect of blood vessels on pancreas development is inhibition of growth and a restriction of final organ size. The signals that mediate this response are independent of blood flow, circulating plasma factors and the provision of nutrients and oxygen, as manipulations of the vasculature lead to similar effects in vivo and in explants cultured in complete medium and ambient oxygen.. These findings seem counterintuitive and run against the well-established notion that blood vessels are positive regulators of tissue growth during development and postnatal life, as well as in pathologies such as cancer. How can our results be reconciled with this view? More specifically, how do our findings fit into the current understanding of pancreas development and ...
TY - JOUR. T1 - Exploring the molecular crosstalk between pancreatic bud and mesenchyme in embryogenesis. T2 - Novel signals involved. AU - Guerriero, Ilaria. AU - De Angelis, Maria Teresa. AU - Dangelo, Fulvio. AU - Leveque, Rita. AU - Savignano, Eleonora. AU - Roberto, Luca. AU - Lucci, Valeria. AU - Mazzone, Pellegrino. AU - Laurino, Simona. AU - Storto, Giovanni. AU - Nardelli, Anna. AU - Sgambato, Alessandro. AU - Ceccarelli, Michele. AU - De Felice, Mario. AU - Amendola, Elena. AU - Falco, Geppino. PY - 2019/10/1. Y1 - 2019/10/1. N2 - Pancreatic organogenesis is a multistep process that requires the cooperation of several signaling pathways. In this context, the role of pancreatic mesenchyme is important to define the epithelium development; nevertheless, the precise space-temporal signaling activation still needs to be clarified. This study reports a dissection of the pancreatic embryogenesis, highlighting the molecular network surrounding the epithelium-mesenchyme interaction. To ...
Biologically active food supplement Suprefort® is the pancreas peptide bioregulator. Protects against pancreatitis and other assorted pancreatic disorders Aids digestive function by supporting lipid and carbohydrate metabolism Serves the same role as peptide bio-regulators developed naturally in the body Helps prevent or mitigate the effects of both type 1 and type 2 diabetes Suprefort® - The pancreas peptide bioregulator.New Russian peptide revolution Maintaining an optimum pancreatic function is essential for its role in two of the bodys key functions: the endocrine system and digestive system. The peptide bioregulator, Superfort, supports the function of the pancreas to avoid pancreatitis that can lead to digestive problems, hormonal imbalance, and abdominal pain. Suprefort is a dietary supplement with natural pancreas peptides. It normalizes the function of the pancreas by reducing its peptide deficiency and restoring protein synthesis inside its cells.The pancreas produces enzymes
The mammalian pancreas is a compound gland of endocrine and exocrine tissues derived from the embryonic endoderm (62). Approximately 90% of the pancreas is exocrine tissue, comprising acinar cells that synthesize and secrete digestive enzymes and ductal cells that secrete and channel the fluid that transports the acinar enzymes to the duodenum. About 1% of the pancreas is endocrine tissue, comprising four principal cell types synthesizing insulin (β-cells), glucagon (α-cells), somatostatin (δ-cells), and pancreatic polypeptide (PP cells) organized into islets scattered throughout the exocrine pancreas. The endocrine and exocrine compartments are structurally and functionally integrated through an islet-acinar portal blood system that facilitates the regulation of acinar cell functions directly by islet peptide hormones (81).. The exocrine-endocrine relationship begins at the inception of pancreogenesis: both tissues are derived from a common endodermal cell lineage (for reviews, see ...
Mesenchyme. Coalescence of the mesenchyme at the level where the dorsal pancreas will form is the first morphological sign of pancreatic development. Removal of the mesoderm, or the fibroblasts within the mesoderm, prior to pancreatic specification results in pancreatic agenesis (53, 107, 164). Mesoderm removal following specification results in a reduction of the total pancreatic size indicating an ongoing requirement for mesoderm signaling to attain complete organ development (94). Interestingly, culturing of pancreatic mesenchyme with other sections of the dorsal endoderm can promote pancreatic differentiation, while mesenchyme from other regions of the anterior-posterior axis does not have this ability (10). This suggests that the mesenchyme provides signals that promote pancreatic specification, yet limits differentiation, thereby allowing expansion of the organ. These signals come in a variety of sources.. Physical interactions between the mesoderm and developing pancreatic bud affect ...
We were quite surprised to find cellular structures that are essentially as old as the organism they reside in, says Salk Vice President, Chief Science Officer Martin Hetzer, senior author and professor. This suggests even greater cellular complexity than we previously imagined and has intriguing implications for how we think about the aging of organs, such as the brain, heart and pancreas. Most neurons in the brain do not divide during adulthood and thus experience a long lifespan and age-related decline. Yet, largely due to technical limitations, the lifespan of cells outside of the brain was difficult to determine. Isotope imaging of different cells inside an islet of Langerhans within the pancreas. Older cells have a yellow-to-pink color scheme, while younger cells exhibit a blue-to-green color pattern. Isotope imaging of different cells inside an islet of Langerhans within the pancreas. Older cells have a yellow-to-pink color scheme, while younger cells exhibit a blue-to-green color ...
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Breaks down nutrients and makes hormones. The pancreas does two main things. It makes fluids that contain enzymes, which break down the nutrients in food - like fats and proteins - so your body can use them. And it makes hormones like insulin to balance your blood sugar levels. Where is the pancreas located? Behind the stomach. The pancreas is about 6 inches long and looks a bit like a tadpole, with a head, body, and tail. It sits in the abdomen, behind the stomach and in front of the spine. What does your pancreas make? Enzymes. Enzymes are proteins that boost the rate of chemical reactions in your body. Without them, processes that can take seconds or minutes would take years. Enzymes in the pancreas combine with bile, a liquid made by the liver, to break down food. You can live without a pancreas. True. Because the pancreas makes insulin, youll develop diabetes if you need to have yours removed. That means you will be dependent on insulin shots (or a pump). Its also hard to manage because ...
Stem or progenitor cells are a promising potential alternative source of pancreatic islets for transplantation in the treatment of juvenile-onset diabetes. However, to derive islets from such cells, it is important to elucidate the mechanisms of normal pancreatic development. Previous work in our laboratory has shown that, contrary to previous thinking, pancreatic mesenchyme when combined with pancreatic epithelium can contribute cells to islets. However, the signals and role of individual tissues involved in this mesenchyme-to-epithelial transition (MET) have yet to be elucidated. The aim of this study was to investigate whether MET can occur in the absence of pancreatic epithelium. Chick and quail eggs were incubated for 4 days and the dorsal pancreatic buds and stomach rudiments were microdissected. Mesenchyme and epithelium of the organ rudiments were separated after collagenase treatment. Separated pancreatic mesenchyme were cultured alone and in combination with stomach (nonpancreatic). After 7
Objective: Pancreas organogenesis is orchestrated by interactions between the epithelium and the mesenchyme, but these interactions are incompletely understood. Here we investigated a role for BMP signalling within the pancreas mesenchyme and found it to be required for the normal development of the mesenchyme as well as the pancreatic epithelium.. Research Design and Methods: We analysed active BMP signalling by immunostaining for phospho-Smad1,5,8 and tested whether pancreas development was affected by BMP inhibition after expression of Noggin and dominant negative BMP receptors in chicken and mouse pancreas.. Results: Endogenous BMP signalling is confined to the mesenchyme in the early pancreas and inhibition of BMP signalling results in severe pancreatic hypoplasia with reduced epithelial branching. Notably, we also observe an excessive endocrine differentiation when mesenchymal BMP signalling is blocked, presumably secondary to defective mesenchyme to epithelium signalling.. Conclusions: We ...
2. Activation of ERK pathway in Pancreatic Acinar Cells (Figure 1). ERK activation is usually monitored by following the dual phosphorylation of the Thr and Tyr residues in the Thr-Glu-Tyr activation sequence brought about by MEK as there are a number of good phosphospecific antibodies directed at this epitope. It can also be shown by phosphorylation of myelin basic protein either in a test tube or by an in gel technique following gel electrophoresis and renaturization. Both Western blots and the in gel kinase procedure reveal the two forms of ERK at approximately 44 and 42 kDa; in fact, the molecules were originally referred to as p42 and p44 MAPK with p42 being what is now referred to as ERK2 and p44 now being ERK1. Using isolated rat or mouse pancreatic acini in vitro, ERK1/2 is activated by CCK, bombesin, substance P, and carbachol, all of which activate G protein coupled receptors coupled to Gq and calcium mobilization but not by secretin or VIP which activate receptors coupled to Gs and ...
TY - JOUR. T1 - Pancreatic acinar cell physiology and function. AU - Miller, L. J.. PY - 1992/1/1. Y1 - 1992/1/1. N2 - The past year has witnessed a marked increase in the complexity of intracellular processes involved in the function of a polarized epithelial cell. The pancreatic acinar cell is perhaps the best studied such cell, and the classical model for stimulus-secretion coupling and secretory protein biosynthesis and export. In this review, I examine several new reports that impact on these themes. I include the identification of newly recognized pancreatic secretagogues; insights into the biochemical basis for hormone receptor affinity states and interconversion, desensitization, and internalization; lipid mediators of secretagogue action; patterns and regulators of intracellular calcium events along with their spatial and temporal patterns; regulated channels, transporters, and junctional communication in the acinar cell and cellular organelles; and new insights into regulated ...
Just as many scientists had given up the search, researchers have discovered that the pancreas does indeed harbor stem cells with the capacity to generate new insulin-producing beta cells.
Have you ever published using Cynomolgus Monkey Pancreatic Epithelial Cells? Submit your publication and earn rewards points which can be used for merchandise & discounts. Please include the product used, your name, email, publication title, author(s), PUBMED ID, Journal and issue in your submission ...
Pancreatic gene transfer could be beneficial to treat many diseases, such as for example diabetes mellitus, cystic fibrosis, persistent pancreatitis, or pancreatic cancer. CMV-GFP reporter cassette, could actually transduce islet and acinar cells, but transgene appearance was dropped 15 times postinjection in relationship with serious lymphocytic infiltration. When HDAds encoding GFP beneath the control of the precise elastase promoter had been used, manifestation was recognized in acinar cells, but similarly, the manifestation almost disappeared thirty days postinjection and lymphocytic infiltration was also noticed. On the other hand, long-term transgene manifestation (>8 weeks) was accomplished with HDAds holding the insulin promoter as well as the secretable alkaline phosphatase as the reporter gene. Notably, transduction from the liver organ, the preferred focus on for adenovirus, was minimal by this path of delivery. These data reveal that HDAds could possibly be useful for pancreatic gene ...
In this protocol, primary embryonic mesenchymal cells are used as feeders for the expansion of definitive endoderm and endocrine progenitor cells. Mesenchymal progenitor cells can promote up to 6-million-fold expansion of embryonic stem cell-derived definitive endoderm cells. These expanded cells are further differentiated into pancreatic progenitor cells.. ...
Cystic tumors of the pancreas today are diagnosed more frequently in clinical practice, mainly due to an increased use of the modern advanced imaging modalities.. Bland cysts of the pancreas most often develop after chronic or acute inflammation of the pancreas. However, the current knowledge concerning the development of cystic neoplasias of the pancreas is still rudimentary.. Histopathologically, 90% of pancreatic cystic neoplasias are represented by four types: serous microcystic (SCN), mucinous cystic (MCN), intraductal papillary mucinous (IPMN) and solid pseudopapillary (SPN) neoplasias. Surgical treatment of these lesions can be highly challenging and occasionally demands complex surgical approaches that should be put in the hands of skilled pancreatic surgeons in experienced high-volume centers.. While some of the described cystic tumors are harmless, such as SCNs of the pancreas, others such as IPMN and MCN harbor relevant malignant potential. The differential diagnosis of these lesions ...
The pancreas is a dual function organ contributing to both blood glucose homeostasis and digestion. These functions are carried out by the endocrine and exocrine compartments of the pancreas, respectively, which derive from common multipotent progenitor cells (MPCs) during embryonic development. The differentiation process for the cells composing both the endocrine and exocrine compartments is highly orchestrated by regulatory transcription factors. Previous work from our lab showed that one such factor, Onecut 1 (Oc1), is essential for initiating endocrine development, proper duct development, and appears necessary for acinar cell development. Using gene expression and physiologic analyses of genetically altered mouse models we have determined that threshold-dependent cooperation between Oc1 and another transcription factor, Pancreatic and duodenal homeobox 1 (Pdx1) in MPCs is necessary for proper endocrine specification, differentiation, maturation, and function. Additionally, we have ...
Researchers tissue-engineered human pancreatic islets that secrete hormones like insulin and successfully treat sudden-onset type 1 diabetes in transplanted mice.
contains a pure and clean source of New Zealand bovine pancreas glandular which is loaded with enzymes, B-vitamins, and minerals designed to support normal blood sugar levels and healthy pancreatic function
The pancreas performs both exocrine and endocrine functions. The exocrine pancreas consists of two parts, the acinar and duct cells. The primary functions of pancreatic acinar cells are to synthesize and secrete digestive enzymes. Stimulation of the cell by secretagogues such as acetylcholine (ACh) and cholecystokinin (CCK) causes the generation of an intracellular Ca2+ signal. This signal, in turn, triggers the fusion of the zymogen granules with the apical plasma membrane, leading to the polarised secretion of the enzymes. The major task of pancreatic duct cells is the secretion of fluid and bicarbonate ions (HCO3-), which neutralize the acidity of gastric contents that enter the duodenum. An increase in intracellular cAMP by secretin is one of the major signals of pancreatic HCO3- secretion. Activation of the CFTR Cl- channel and the CFTR-dependent Cl-/HCO3- exchange activities is responsible for cAMP-induced HCO3- secretion ...
To understand the development of the human pancreas better, we studied the expression and regulation of insulin, insulin-like growth factor-II (IGF-II) and transforming growth factor-α (TGF-α) genes in the human fetal pancreas and islet-like cell clusters (ICC) from the second trimester human fetuses. Northern blot analysis revealed an abundant expression of IGF-II, insulin and TGF-α mRNAs in the intact pancreas and the cultured ICCs. Furthermore, transcripts for insulin receptor, type-1 and -2 IGF receptors, and GH receptor could be amplified by polymerase chain reaction analysis from the pancreas and the ICCs. With in-situ hybridization, IGF-II mRNA was found in abundance in both the exocrine and endocrine pancreas, exceeding the amount of insulin mRNA. In ICCs, insulin mRNA-containing cells were present as small clusters in the periphery and in the centre of the clusters corresponding to the immunolocation of insulin. The ICCs also contained many epidermal growth factor-, insulin- and ...
A 58-kD cis-Golgi protein has been identified by generating polyclonal antibodies against heavy (cis) Golgi subfractions. Total microsomes isolated from rat pancreatic homogenates were subfractionated to yield a rough microsomal fraction (B1) and three smooth membrane subfractions (B2-B4) enriched in cis-, middle, and trans-Golgi elements, respectively. The heavy (cis) subfraction, B2 (d = 1.17 g/ml), was fractionated by Triton X-114 phase separation, and the proteins recovered in the detergent phase were used to immunize rabbits. One of the anti-B2 antibodies obtained gave a Golgi-staining pattern when screened by immunofluorescence on normal rat kidney cells and mouse RPC 5.4 myeloma cells. In rat pancreatic exocrine cells the antibody reacted with the plasmalemma as well as elements in the Golgi region. By immunoelectron microscopy, the antigen recognized by anti-B2 IgG was found to be restricted to cis-Golgi elements in myeloma cells where it was concentrated in the fenestrated cis-most ...
Purified Human Pancreas Membrane Diabetic Disease Lysate from Creative Biomart. Human Pancreas Membrane Diabetic Disease Lysate can be used for research.
Foods good for pancreas : What is Pancreatic Inflammation? Diet suitable for inflammation of the pancreas.Worldwide, more than 10,000..
The 86-year-old justice just wrapped up weeks of treatment after a new cancerous tumor was found on her pancreas. Doctors say theres no evidence of cancer elsewhere.