The recent increase in bacterial resistance to antibiotics has promoted the exploration of novel antibacterial materials. As a result, many researchers are undertaking work to identify new lantibiotics because of their potent antimicrobial activities. The objective of this study was to provide details of a lantibiotic-like gene cluster in Paenibacillus elgii B69 and to produce the antibacterial substances coded by this gene cluster based on culture screening. Analysis of the P. elgii B69 genome sequence revealed the presence of a lantibiotic-like gene cluster composed of five open reading frames (elgT1, elgC, elgT2, elgB, and elgA). Screening of culture extracts for active substances possessing the predicted properties of the encoded product led to the isolation of four novel peptides (elgicins AI, AII, B, and C) with a broad inhibitory spectrum. The molecular weights of these peptides were 4536, 4593, 4706, and 4820 Da, respectively. The N-terminal sequence of elgicin B was Leu-Gly-Asp-Tyr, which
This paper addresses changes in plant gene expression induced by inoculation with plant-growth-promoting rhizobacteria (PGPR). A gnotobiotic system was established with Arabidopsis thaliana as model plant, and isolates of Paenibacillus polymyxa as PGPR. Subsequent challenge by either the pathogen Er …
A Gram-stain-positive, aerobic, endospore-forming bacterium, designated CAU 1055T, was isolated from soil and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Paenibacillus and was most closely related to Paenibacillus contaminans CKOBP-6T (similarity, 95.2 %) and Paenibacillus terrigena A35T (similarity, 95.2 %). The levels of 16S rRNA gene sequence similarity with other species of the genus Paenibacillus , including the type species of the genus, Paenibacillus polymyxa IAM 13419T (similarity, 91.7 %), were all <94.6 %. Strain CAU 1055T contained MK-7 as the only isoprenoid quinone and anteiso-C15 : 0 and iso-C16 : 0 as the major fatty acids. The cell-wall peptidoglycan of strain CAU 1055T contained meso-diaminopimelic acid. The polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lysyl
A novel strain of the genus Paenibacillus, KFC91T, was isolated from the Kafni glacier of the Himalayas. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KFC91T clustered with Paenibacillus antarcticus LMG 22078T (98.9 %) and Paenibacillus macquariensis LMG 6935T (98.7 %). The cell wall peptidoglycan contained meso-diaminopimelic acid as the diamino acid, anteiso-C15 : 0 as the predominant fatty acid and MK-7 as the major isoprenoid quinone. Based on the phylogenetic analysis, chemotaxonomic characteristics and other phenotypic traits, strain KFC91T was assigned to the genus Paenibacillus. DNA-DNA hybridization experiments of KFC91T with P. antarcticus and P. macquariensis showed reassociation values of 39 and 52 %, respectively. Thus, it is proposed that strain KFC91T should be assigned the status of a novel species of the genus Paenibacillus and the name proposed is Paenibacillus glacialis sp. nov., with KFC91T (=NCCB 100252T =DSM 22343T) as the type strain.
Four Gram-stain-positive bacterial strains, designated 6R2T, 6R18, 3T2 and 3T10, isolated from seeds of hybrid maize (Zea mays L., Jingke 968) were investigated using a polyphasic taxonomic approach. Cells were aerobic, motile, spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolates may represent a novel species of the genus Paenibacillus, the four closest neighbours being Paenibacillus lautus NRRL NRS-666T (97.1 % similarity), Paenibacillus glucanolyticus DSM 5162T (97.0 %), Paenibacillus lactis MB 1871T (97.0 %) and Paenibacillus chibensis JCM 9905T (96.8 %). The DNA G+C content of strain 6R2T was 51.8 mol%. Its polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids were anteiso-C15 : 0 and iso-C14 : 0. Strains 6R2T, 6R18, 3T2 and 3T10 were clearly distinguished from the above type strains using phylogenetic
Paenibacillus is a genus of facultative anaerobic, endospore-forming bacteria, originally included within the genus Bacillus and then reclassified as a separate genus in 1993. Bacteria belonging to this genus have been detected in a variety of environments, such as: soil, water, rhizosphere, vegetable matter, forage and insect larvae, as well as clinical samples. The name reflects: Latin paene means almost, so the paenibacilli are literally almost bacilli. The genus includes P. larvae, which is known to cause American foulbrood in honeybees, the P. polymyxa, which is capable of fixing nitrogen, so is used in agriculture and horticulture, the Paenibacillus sp. JDR-2 which is known to be a rich source of chemical agents for biotechnology applications, and pattern-forming strains such as P. vortex and P. dendritiformis discovered in the early 90s, which are known to develop complex colonies with intricate architectures as shown in the pictures: Picture 1: A colony generated by the chiral ...
It has come to our attention that we have used Asp, rather than the correct annotation of Asn, to indicate Asparagine throughout the text [1].. In the abstract this is corrected to: The N-terminal sequence of elgicin B was Leu-Gly-Asn-Tyr, which corresponded to the partial sequence of the peptide ElgA encoded by elgA.. In the Results section, subsection Analysis of N-terminal amino acid sequence, all instances of Asp should be replaced with Asn.. We regret any inconvenience that this inaccuracy in the text might have caused. ...
Background: In previous studies, the gram-positive firmicute genus Paenibacillus was found with significant abundances in nests of wild solitary bees. Paenibacillus larvae is well-known for beekeepers as a severe pathogen causing the fatal honey bee disease American foulbrood, and other members of the genus are either secondary invaders of European foulbrood or considered a threat to honey bees. We thus investigated whether Paenibacillus is a common bacterium associated with various wild bees and hence poses a latent threat to honey bees visiting the same flowers. Results: We collected 202 samples from 82 individuals or nests of 13 bee species at the same location and screened each for Paenibacillus using high-throughput sequencing-based 16S metabarcoding. We then isolated the identified strain Paenibacillus MBD-MB06 from a solitary bee nest and sequenced its genome. We did find conserved toxin genes and such encoding for chitin-binding proteins, yet none specifically related to foulbrood ...
The detection and control of American Foulbrood (AFB) is made more challenging due to a lack of evidence regarding the virulence mechanisms employed by this honeybee pathogen. Whilst incidence of this pathogen within the UK has recently declined, ~100 colonies were identified as infected with AFB in 2011 (to end of September), so AFB should still be considered a serious threat to honeybee health. It is known that within the species many phenotypes exist, and the infection caused by the phenotypes differs greatly. This PhD thesis presents several advances towards a greater understanding of the intra-specific differences occurring within the species. Chapter 2 evaluates the use of 16S rRNA sequencing as a method of Paenibacillus larvae identification, as well as exploring the use of this ribosomal subunit for differentiation of the species. The sequencing of two housekeeping (purH and PyrE) genes assesses the potential of a Multi Locus Sequence Typing (MLST) method, as a means of subspecies ...
FIG. 4. Bacterial mobile group II intron-mediated gene disruption of wsfP in P. alvei CCM 2051T. (A) Screening of Cm-resistant P. alvei CCM 2051T colonies for intron insertion by in situ PCR using primers KO_wsfP_control_for_1 (→) and KO_wsfP_control_rev_1 (←). A PCR product obtained from a wild-type colony (lane 1), a PCR fragment obtained from a wsfP mutant (lane 2), and PCR products obtained from a colony containing both wild-type and intron-inserted wsfP (lane 3) are shown. (B) Schematic drawing of the wsfP gene with (bottom) and without (top) intron insertion, indicating the positions of primers KO_wsfP_control_for_1 (→) and KO_wsfP_control_rev_1 (←). ...
From: paleopsych-bounces at paleopsych.org [mailto:paleopsych-bounces at paleopsych.org] On Behalf Of Thrst4knw at aol.com Sent: Monday, April 18, 2005 3:14 PM To: paleopsych at paleopsych.org Cc: ToddStark at aol.com; HowlBloom at aol.com Subject: [Paleopsych] Intelligent Bacteria http://www.world-science.net/exclusives/050418_bactfrm.htm Intelligent bacteria? For the most primitive beings in the web of life, some researchers claim, simple might not mean stupid. Posted April 18, 2005 Special to World Science Bacteria are by far the simplest things alive, at least among things generally agreed on as being alive. Next to one of these single-celled beings, one cell of our bodies looks about as complex as a human does compared to a sponge. ,http://www.hometown.aol.com/scipage/images/plate-CT-docu0043b.jpg, A colony of Paenibacillus dendritiformis bacteria, which some researchers say can organize themselves into different types of extravagant formations to maximize food intake for given ...
Bacteria have developed a variety of mechanisms for surviving harsh environmental conditions, nutrient stress and overpopulation. Paenibacillus dendritiformis produces a lethal protein (Slf) that is able to induce cell death in neighbouring colonies and a phenotypic switch in more distant ones. Slf is derived from the secreted precursor protein, DfsB, after proteolytic processing. Here, we present new crystal structures of DfsB homologues from a variety of bacterial species and a surprising version present in the yeast Saccharomyces cerevisiae. Adopting a four-helix bundle decorated with a further three short helices within intervening loops, DfsB belongs to a non-enzymatic class of the DinB fold. The structure suggests that the biologically active Slf fragment may possess a C-terminal helix rich in basic and aromatic residues that suggest a functional mechanism akin to that for cationic antimicrobial peptides. Structures of the DfsB Protein Family Suggest a Cationic, Helical Sibling Lethal ...
Paenibacillus dendritiformis bacterial colonies secrete a protein that, once activated, regulates growth and preserves nutrients in nearby colonies, according to physicist Eshel Ben-Jacob at Tel Aviv University in Tel Aviv, Israel, and his collaborators, most of whom are physicists. However, the protein does not account for the intricate branching patterns that develop among those colonies and that help make them so fascinating to study, he says. Details appear in the April 6, 2010 Proceedings of the National Academy of Sciences (107:6258-6263).
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Homologous recombination (HR) is essential for the accurate repair of DNA double-strand breaks (DSBs), potentially lethal lesions. HR takes place in the late S-G2 phase of the cell cycle and involves the generation of a single-stranded region of DNA, followed by strand invasion, formation of a Holliday junction, DNA synthesis using the intact strand as a template, branch migration and resolution. It is investigated that RecA/Rad51 family proteins play a central role. The breast cancer susceptibility protein Brca2 and the RecQ helicase BLM (Bloom syndrome mutated) are tumor suppressors that maintain genome integrity, at least in part, through HR ...
If youve been reading this blog for a bit, you know that Ive had run-ins with American Foulbrood (AFB) for two years in a row. I run naturally treated, anti
Phenotypic studies, as well as the reaction of Paenibacillus durum genomic DNA with a 16S ribosomal DNA (sequence of variable regions V1 to V4)-based Paenibacillus azotofixans-specific PCR system and oligonucleotide probe, the presence of sequences homologous to Klebsiella pneumoniae nifKDH in both P. durum and P. azotofixans, and the results of DNA-DNA hybridization experiments performed with the P. durum and P. azotofixans type strains and one additional P. durum strain, showed that these two species form a homogeneous group. In addition, evidence was found for the presence of nif genes in P. durum, and P. durum was shown to fix atmospheric nitrogen. Therefore, the names P. durum and P. azotofixans should be considered synonyms. As P. durum was capable of fixing nitrogen and fixation without inhibition by nitrate is a major characteristic of the group, we propose that P. durum be included in the species P. azotofixans.
Deo, Namita and Natarajan, KA and Somasundaran, P (2001) Mechanisms of adhesion of Paenibacillus polymyxa onto hematite, corundum and quartz. In: International Journal of Mineral Processing, 62 (1-4). pp. 27-39. Natarajan, KA and Deo, Namita (2001) Role of bacterial interaction and bioreagents in iron ore flotation. In: International Journal of Mineral Processing, 62 (1-4). pp. 143-157. Deo, Namita and Natarajan, KA and Rao, Hanumantha K and Forssberg, KSE (1999) Biodegradation of some organic reagents from mineral process effluents. In: Biohydrometallurgy and the Environment Toward the Mining of the 21st Century - Proceedings of the International Biohydrometallurgy Symposium, JUN 20-23, 1999, Madrid, Spain. Deo, Namita and Vasan, SS and Modak, Jayant M and Natarajan, KA (1999) Selective biodissolution of calcium and iron from bauxite in the presence of Bacillus polymyxa. In: Biohydrometallurgy and the Environment Toward the Mining of the 21st Century - Proceedings of the International ...
Transfer of Bacillus ehimensis and Bacillus chitinolyticus to the genus Paenibacillus with emended descriptions of Paenibacillus ehimensis comb. nov. and Paenibacillus chitinolyticus comb. novLEE, Jung-Sook; PYUN, Yu-Ryang; KYUNG SOOK BAE et al.International journal of systematic and evolutionary microbiology (Print). 2004, Vol 54, pp 929-933, issn 1466-5026, 5 p., 3Article ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
The inulin-binding function has been demonstrated in the case of the cycloinulo-oligosaccharide fructanotransferase from Paenibacillus macerans (Bacillus macerans) by Lee et al. (2004) FEMS Microbiol Lett 234:105-10. (PMID:15109727 ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Sigma-Aldrich offers abstracts and full-text articles by [Chakrit Tachaapaikoon, Somboon Tanasupawat, Patthra Pason, Somphit Sornyotha, Rattiya Waeonukul, Khin Lay Kyu, Khanok Ratanakhanokchai].
Paenibacillus larvae (White 1906) Ash, Priest and Collins 1994VL emend. Heyndrickx, Vandemeulebroecke, Hoste, Janssen, Kersters, De Vos, Logan, Ali and Berkeley 1996 ...
Publications figure showing the NMR solution-structure of the tridecaptin A1:lipid II complex Recommended in F1000 Prime. 11. Lipopeptides from Bacillus and Paenibacillus spp.: A Gold Mine of Antibiotic Candidates. S. A. Cochrane and J. C. Vederas. Med. Res. Rev. 2016, 36, 4. 10. Synthesis of Tridecaptin-Antibiotic Conjugates with in Vivo Activity Against Gram-Negative Bacteria. S. A. Cochrane, X. Li, S. He, M. Yu, M. Wu and J. C. Vederas. J. Med. Chem. 2015, 58, 9779. ...
Paenibacillus pabuli clone JS-4F 16S ribosomal RNA gene, partial sequence;16S-23S internal transcribed spacer, complete sequence; and 23S ribosomalRNA gene, partial ...
American foulbrood (AFB, Histolysis infectiosa perniciosa larvae apium, Pestis americana larvae apium), caused by the spore-forming Paenibacillus larvae ssp. larvae (formerly classified as Bacillus larvae), is the most widespread and destructive of the bee brood diseases. Paenibacillus larvae is a rod-shaped bacterium, which is visible only under a high power microscope. Larvae up to 3 days old become infected by ingesting spores that are present in their food. Young larvae less than 24 hours old are most susceptible to infection. Spores germinate in the gut of the larva and the vegetative form of the bacteria begins to grow, taking its nourishment from the larva. Spores will not germinate in larvae over 3 days old. Infected larvae normally die after their cell is sealed. The vegetative form of the bacterium will die but not before it produces many millions of spores. Each dead larva may contain as many as 100 million spores. This disease only affects the bee larvae but is highly infectious and ...
Over the course of this two year project, PKPB1 was evaluated as a biocontrol for fungal diseases of vegetable crops in a greenhouse hydroponic system. Trials to assess efficacy against Fusarium, Pythium, powdery mildew and gummy stem blight pathogens in greenhouse cucumber and pepper production were conducted at Alberta Research Council commercial-scale greenhouse facilities.. Three experiments were conducted to evaluate PKPB1 for Pythium control on cucumber. It was found that PKPB1 delayed development of Pythium root rot in the first two weeks after inoculation, showed similar effect at the end of six weeks after treatments were applied, indicating that PKPBI was as effective as a commercial product (Mycostop®) in suppression of root disease.. Two experiments were conducted on powdery mildew control on cucumber. Foliar application of PKPB1 spore suspension reduced the severity of powdery mildew on cucumber plants infected under natural conditions but the effect was not significant compare to ...
Methane is metabolized principally by methanotrophs and methanogens in the global carbon cycle. Methanotrophs consume methane as the only source of carbon, while methanogens produce methane as a metabolic byproduct. Methylotrophs, which are microorganisms that can obtain energy for growth by oxidizing one-carbon compounds, such as methanol and methane, are situated between methanotrophs and methanogens. Methanogens can obtain energy for growth by converting a limited number of substrates to methane under anaerobic conditions. Three types of methanogenic pathways are known: CO2 to methane [MD:M00567], methanol to methane [MD:M00356], and acetate to methane [MD:M00357]. Methanogens use 2-mercaptoethanesulfonate (CoM; coenzyme M) as the terminal methyl carrier in methanogenesis and have four enzymes for CoM biosynthesis [MD:M00358]. Coenzyme B-Coenzyme M heterodisulfide reductase (Hdr), requiring for the final reaction steps of methanogenic pathway, is divided into two types: cytoplasmic HdrABC in ...
TY - JOUR. T1 - Paenidigyamycin A, Potent Antiparasitic Imidazole Alkaloid from the Ghanaian Paenibacillus sp. DE2SH. AU - Osei, Enoch. AU - Kwain, Samuel. AU - Mawuli, Gilbert Tetevi. AU - Anang, Abraham Kwabena. AU - Owusu, Kofi Baffour-Awuah. AU - Camas, Mustafa. AU - Camas, Anil Sazak. AU - Ohashi, Mitsuko. AU - Alexandru-Crivac, Cristina-Nicoleta. AU - Deng, Hai. AU - Jaspars, Marcel. AU - Kyeremeh, Kwaku. N1 - KK wishes to thank the Centre for African Wetlands (CAW), University of Ghana, for providing seed funding to enable the collection of soil samples for microbe isolation and a TWAS Research Grant Award_17-512 RG/CHE/AF/AC_G. K.K. is also very grateful to the Cambridge-Africa Partnership for Research Excellence (CAPREx), which is funded by the Carnegie Corporation of New York, for a Postdoctoral Fellowship. K.K. also appreciates the Cambridge-Africa ALBORADA Research Fund for support and MRC African Research Leaders MR/S00520X/1 Award. S.K. wishes to thank the Carnegie BANGA-Africa ...
Read Raw sugarcane bagasse as carbon source for xylanase production by Paenibacillus species: a potential degrader of agricultural wastes, Environmental Science and Pollution Research on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
A fully bleached commercial acid dissolving pulp was treated with two endoglucanases, one obtained from Paenibacillus barcinonensis (B) and the other one produced from Cerrena unicolor (F) with the intention to improve cellulose reactivity and processability in the viscose process. B cellulose was tested under 120 U/g oven dry pulp (odp) and the F cellulase under two conditions, 12 and 60 U/g odp. In addition, a purification stage, consisting in a cold caustic extraction (CCE) of 9 % w/v NaOH, was applied before or after the enzymatic treatment in order to reduce the amount of hemicellulose and improve the action of enzymes. The treated pulps were evaluated in terms of brightness, viscosity, water retention value, fibre morphology, carbohydrate composition, Fock solubility and NMR. In general, results revealed that both endoglucanases improved cellulose reactivity, albeit in a different way; thus, B caused no scissions in the cellulose chain and no significant reduction in fibre length, whereas ...
This came in the email this morning. Sorry if this has already been discussed. I did a search and only 1 post came up with 1 reply...
Colistin eller polymyxin E er et antibiotikum, der produceres af visse stammer af Paenibacillus polymyxa var. colistinus. Colistin er en blanding af to cykliske polypeptider colistin A og B Da colistin binder til de Gram-negative bakteriers lipopolysaccharid (LPS, også kaldet endotoxin), er det som antibiotikum effektivt mod de fleste Gram-negative baciller. Colistin er en årtier gammel medicin, der faldt i unåde på grund af sin nefrotoksicitet. Det er fortsat et af de sidste forsvar over for multiresistente Pseudomonas aeruginosa, Klebsiella pneumoniae og Acinetobacter. NDM-1 metallo-beta-lactamase multidrug-resistente enterobakterier har også vist en modtagelighed over for colistin. Colistin blev tidligere givet som intramuskulær injektion mod Gram-negative infektioner, men benyttes nu mest som aerosol ved cystisk fibrose.[1] Colistinresistens er blevet identificeret både i Europa, USA og Kina i Escherichia coli stamme SHP45, hvor resistensen er båret af et plasmid med mcr-1-genet.[2] ...
Image: Brood infected with European Foulbrood. Photo by M.V. Smith. Credit: Zach Huang American and European Foulbrood are...
naturally give rise to more classes of drug and PhD., MRCVS; Mavromihali 154, Athens 11472, Greece; Tel.: 0030-6972-314842; E-mail: Trakia Journal of Sciences, Vol. 4, No.1, 2006 SARIDAKI-PAPAKONSTADINOU M., et al. Verzegnassi et al., 2003; Wang, 2004), with the concomitant increase in proportion of honey samples reported as positive (Heering et al., 1998; Verzegnassi et al., 2003; Vinas et passed through glass wool (SUPELCO) filters before it was further purified through a Baker organisms, suffer the consequence of disease, column 10C18 (SUPELCO) suitable for and beekeepers often use either preventive or separating organic compounds. The column therapeutic antimicrobials to protect bee was washed with 10 ml distilled water and health and levels of honey production. The further cleaned by the passing of high speed tetracyclines, among other agents, have been used consistently for treating foulbrood were removed by passing through the column caused by Paenibacillus larvae (Evans, 2003). of 5 ml ...
Honeybees are vitally important, and not just for their honey - theyre important pollinators of food crops. But in recent years, theres been a serious decline in the honeybee population. Among the biggest culprits are bacteria called American foulbrood.. The bacterias microscopic spores spread quickly from beehive to beehive, killing the bee larvae. The spores can survive for many years. Fortunately, if the bacteria are detected early enough, antibiotics can save the hive.. For decades, many states have had bee inspectors on their payrolls in an effort to keep the bacteria under control. But inspecting beehives for bacteria is a time-consuming job for humans, who must open each bee colony to look for the infection. A human bee inspector might take a full day to inspect 50 beehives. [source: Coren]. Fortunately, with proper training, the same keen sense of smell that enables dogs to detect illegal drugs can be used to nose out the foulbrood bacteria. In the late 1970s, the state of Maryland ...
De Graaf, D.C., Alippi, A.M., Antunez, K., Aronstein, K.A., Budge, G., Dekoker, D., De Smet, L., Dingman, D.W., Evans, J.D., Foster, L.J., Funfhaus, A., Garcia-Gonzalez, E., Gregoric, A., Human, H., Murray, K.D., Nguyen, B., Poppinga, L., Spivak, M., Vanengelsdorf, D., Wilkins, S., Genersch, E. 2013. In Vincent Dietemann, James D. Ellis and Peter Neumann (Editors) Standard methods for Apis mellifera pests and pathogens, Selected techniques and protocols in American foulbrood research, The COLOSS BEEBOOK, Volume II ...
Authors: Shinya, Shoko; Nishimura, Shigenori; Fukamizo, Tamo. Citation: Shinya, Shoko; Nishimura, Shigenori; Kitaoku, Yoshihito; Ohnuma, Takayuki; Numata, Tomoyuki; Kimoto, Hisashi; Kusaoke, Hideo; Fukamizo, Tamo. Mechanism of chitosan recognition by CBM32 carbohydrate-binding modules from a Paenibacillus sp. IK-5 chitosanase/glucanase Biochem. J. ., .-. (2016).. Assembly members: ...
Between 2008 and 2014, the U.S. fluid milk market showed positive dynamics. It rose from 189 billion pounds in 2008 to 205 billion pounds in 2014, expanding with a CAGR of +1.4%.
The food enzyme cyclomaltodextrin glucanotransferase ((1→4)‐α‐d‐glucan:(1→4)‐α‐d‐glucan 4‐α‐d‐[(1→4)‐α‐d‐glucano]‐transferase; EC 2.4.1.19) is produced with the non‐genetically modified Paenibacillus illinoisenis strain 107 by Hayashibara Co., Ltd. The cy .... ...
TY - JOUR. T1 - Paenibacillus polymyxa antagonizes oomycete plant pathogens Phytophthora palmivora and Pythium aphanidermatum. AU - Timmusk, S. AU - Van West, P. AU - Gow, N A R. AU - Huffstutler, R Paul. PY - 2009/5. Y1 - 2009/5. N2 - AIM: To find sustainable alternatives to the application of synthetic chemicals for oomycete pathogen suppression. METHODS AND RESULTS: Here, we present experiments on an Arabidopsis thaliana model system in which we studied the antagonistic properties of rhizobacterium Paenibacillus polymyxa strains towards the oomycete plant pathogens Phytophthora palmivora and Pythium aphanidermatum. We carried out studies on agar plates, in liquid media and in soil. Our results indicate that P. polymyxa strains significantly reduced P. aphanidermatum and P. palmivora colonization in liquid assays. Most plants that had been treated with P. polymyxa survived the P. aphanidermatum inoculations in soil assays. CONCLUSIONS: The antagonistic abilities of both systems correlated well ...
Polymyxin E, produced by Paenibacillus polymyxa, is an important antibiotic normally against Gram-negative pathogens. In this study, we found that polymyxin E can kill its producer P. polymyxa, a Gram-positive bacterium, by disrupting its cell membrane. Membrane damage was clearly revealed by detecting the leakage of intracellular molecules. The observation using scanning electron microscopy also supported that polymyxin E can destroy the cell membrane and cause an extensive cell surface alteration. On the other hand, divalent cations can give protection against polymyxin E. Compared with Mg2+, Ca2+ can more effectively alleviate polymyxin E-induced damage to the cell membrane, thus remarkably increasing the P. polymyxa survival. Our findings would shed light on a not yet described bactericidal mechanism of polymyxin E against Gram-positive bacteria and more importantly the nature of limited fermentation output of polymyxin E from P. polymyxa.
Morgellons Disease Is a Fungi (Paenibacillus odorifer) & The Cure - highly contagious Shingles, Lyme Disease is Morgellons Disease & Morgellons Disease Is a Fungi (Paenibacillus odorifer) - highly
Category: Animal, Bacillus, Probiotics. Paenibacillus polymyxa is a Gram-positive bacterium capable of fixing nitrogen. The species may also beknown as Bacillus polymyxa. It is found in soil, plant roots, and marine sediments.Bacillus polymyxa is a Gram-positive bacterium capable of fixing nitrogen.. ...
Douglas W. Dingman, an experiment station microbiologist, said that about one-third of the human diet relies on bees, either directly or indirectly, for pollination of crops by bees, and that foulbrood, if left unchecked, could leave major segments of the nations food supply in tatters.|br/||br/|Dingman said that American foulbrood cant be blamed for whats become known as colony collapse disorder, or CCD, a still-mysterious, worldwide phenomenon in which a hives worker bees fly off, never to be seen again.|br/||br/|Many biologists, he said, suspect that CCD is caused by several factors working together, including insecticides, climate change, viral and bacterial diseases, malnutrition and mites.|br/||br/|American foulbrood, which occurs worldwide, is so named because the hives brood chambers, where the eggs develop into adults, become foul-smelling when the infected larva turn into a gooey mass.|br/||br/|
With so much attention on varroa destructors and nicotine-flavoured pesticides, we sometimes forget some of the other scourges of modern beekeeping. Not long ago, American Foulbrood (AFB) was the worst thing that could happen to your bees. If you were caught with the disease, an inspector might set your colonies upon a pit of fire. Your…
phdthesis{b7d1dc35-566e-49a3-b319-ba8b54b7279f, abstract = {The organization and regulation of genes for tetrapyrrole synthesis were studied in low G+C Gram-positive bacteria. Clusters of genes encoding enzymes for tetrapyrrole biosynthesis were cloned from Bacillus sphaericus, Bacillus stearothermophilus, Brevibacillus brevis and Paenibacillus macerans. The structure of the hem gene clusters was compared to that of other Gram-positive bacteria. The hemEHY operon required for protoheme IX synthesis from uroporphyrinogen III (UroIII) is conserved among low G+C Gram-positive bacteria. Mainly aerobic Bacillus, Brevibacillus and Stapylococcus species have a conserved organization of the genes hemAXCDBL, required for biosynthesis of UroIII from glutamyl-tRNA. In P. macerans and Clostridium species, the hem genes for UroIII synthesis are also closely linked but their organization is different: there is no hemX gene and the gene cluster also contains genes, cysGB and cysGA-hemD, encoding the enzymes ...
Paenibacillus macerans glutamyl-tRNA reductase (hemA), siroheme synthase(cysG(B)), porphobilinogen deaminase (hemC), and uroporphyrinogen-IIImethyltransferase/uroporphyrinogen-III synthase (cysG(A)-hemD) genes,complete cds; and 5-aminolevulinic acid dehydratase (hemB) gene, ...
Background Melissococcus plutonius is an entomopathogenic bacterium that causes European foulbrood (EFB), a honeybee (Apis mellifera L.) disease that necessitates quarantine in some countries. In Czechia, positive evidence of EFB was absent for almost 40 years, until an outbreak in the Krkonose Mountains National Park in 2015. This occurrence of EFB gave us the opportunity to study the epizootiology of EFB by focusing on the microbiome of honeybee workers, which act as vectors of honeybee diseases within and between colonies. Methods The study included worker bees collected from brood combs of colonies (i) with no signs of EFB (EFB0), (ii) without clinical symptoms but located at an apiary showing clinical signs of EFB (EFB1), and (iii) with clinical symptoms of EFB (EFB2). In total, 49 samples from 27 honeybee colonies were included in the dataset evaluated in this study. Each biological sample consisted of 10 surface-sterilized worker bees processed for DNA extraction. All subjects were analyzed using
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http://www.fda.gov/Drugs/DrugSafety/FungalMeningitis/default.htm CDC and FDA Laboratory-Confirmed Organisms from Product Samples Laboratory-Confirmed Organisms from Product Samples Associated with NECC Recalled Lots of Betamethasone, Cardioplegia, and Triamcinolone Solutions Medication Lot Number Bacterial and Fungal Contamination Betamethasone 6 mg/mL injectable -5 mL per vial 08202012@141 Paenibacillus pabuli/amolyticus, Bacillus idriensis, Bacillus flexus, Bacillus simplex, ...