TY - JOUR. T1 - Phenoxazine derivatives induce caspase-independent cell death in human glioblastoma cell lines, A-172 and U-251 MG. AU - Shirato, Ken. AU - Imaizumi, Kazuhiko. AU - Abe, Akihisa. AU - Tomoda, Akio. PY - 2007/1. Y1 - 2007/1. N2 - The apoptotic effects of 2-amino-4,4α-dihydro-4α, 7-dimethyl-3H-phenoxazine-3-one (Phx-1) and 2-amino-phenoxazine-3-one (Phx-3) on human glioblastoma cell lines, A-172 and U-251 MG were studied. These phenoxazines extensively decreased the viability of A-172 and U-251 MG cells (IC50 of Phx-1: 60 μM, in both lines; IC50 of Phx-3: 10 and 3 μM, for A-172 and U-251 cells, respectively). Phx-1 and Phx-3 increased the population of annexin V and PI double-positive cells in A-172 and U-251 MG cells, resulting in cell death at late stage apoptosis/necrosis. The activities of caspase-3/7 were greatly increased in A-172 and U-251 MG cells treated with Phx-1 or Phx-3. However, a pan-caspase inhibitor, z-VAD-fmk, failed to reverse the antiproliferative and ...
The report generally describes phenoxazine, examines its uses, production methods, patents. Phenoxazine market situation is overviewed; phenoxazine
Brilliant cresyl blue definition at Dictionary.com, a free online dictionary with pronunciation, synonyms and translation. Look it up now!
Objectives: To evaluate the performance of three rapid low-cost methods for the detection of resistance to first-line drugs in Mycobacterium tuberculosis.. Methods: One hundred M. tuberculosis clinical isolates were tested by the nitrate reductase assay (NRA), the MTT test and the resazurin microtitre assay (REMA), and the results compared with those obtained with the gold standard proportion method (PM) on Löwenstein Jensen medium.. Results: The results using the three methods showed a good sensitivity and specificity between 94% and 100% for the detection of rifampicin and isoniazid resistance. Specificity for ethambutol and streptomycin using MTT and resazurin was low (58-89%). In contrast, NRA showed a good agreement for all first-line drugs tested.. Conclusions: This study shows a high level of agreement of these three low-cost methods compared with the PM for rapid detection of rifampicin and isoniazid resistance. However, more standardization is needed for ethambutol and streptomycin ...
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7-Benzyl-3- tert -butyl-1-phenyl-6,7-dihydro-1H,4H -pyrazolo[3,4- d][1,3]oxazine, C22H25N3O, (I), and 3- tert -butyl-7-(4-methylbenzyl)-1-phenyl-6,7-dihydro-1H,4H -pyrazolo[3,4- d][1,3]oxazine, C23H27N3O, (II), are isomorphous in the space group P21, and molecules are linked into chains by C,H...O hydrogen bonds. In each of 3- tert -butyl-7-(4-methoxybenzyl)-1-phenyl-6,7-dihydro-1H,4H -pyrazolo[3,4- d][1,3]oxazine, C23H27N3O2, (III), which has cell dimensions rather similar to those of (I) and (II), also in P21, and 3- tert -butyl-1-phenyl-7-[4-(trifluoromethyl)benzyl]-6,7-dihydro-1H,4H -pyrazolo[3,4- d][1,3]oxazine, C23H24F3N3O, (IV), there are no direction-specific interactions between the molecules. In 3- tert -butyl-7-(4-nitrobenzyl)-1-phenyl-6,7-dihydro-1H,4H -pyrazolo[3,4- d][1,3]oxazine, C22H24N4O3, (V), a combination of C,H...O and C,H...N hydrogen bonds links the molecules into complex sheets. There are no direction-specific interactions between the molecules of 3- tert ...
A novel approach to design selective spleen tyrosine kinase (Syk) inhibitors is described. Inhibition of spleen tyrosine kinase has attracted much attention as a mechanism for the treatment of autoimmune diseases such as asthma, rheumatoid arthritis,
The objectives of this study were: 1) To determine the effect of oocyte retrieval techniques (slicing and aspiration) on the quality and quantity of cattle oocytes, 2) To evaluate the effect of different concentrations of hormones on the maturational rate of cattle oocytes selected by brilliant cresyl blue staining, 3) To evaluate fertilization rate and cleavage/embryonic development of oocytes with or without cumulus cells, and 4) To compare the effect of fresh and frozen thawed semen on the fertilization rate of cattle oocytes. In Experiment 1: oocytes were recovered from abattoir derived ovaries using slicing and aspiration. The recovered oocytes were exposed for 90 minutes to 26μM of brilliant cresyl blue (BCB) stain and classified according to the colour of their cytoplasm: BCB+ (oocytes with blue cytoplasm) and BCB- (unstained oocytes). There was no difference (P,0.05) in the quality of oocytes recovered using slicing (60.7 %) or aspiration (53.7 %) techniques. In experiment 2: The BCB ...
2-isopropyl-5,5-dimethyl-5,6-dihydro-2H-1,3-oxazine - chemical structural formula, chemical names, chemical properties, synthesis references
3,4-dihydro-3-phenyl-2H-1,3-benzoxazines derived from phenol-, resorcinol-, and phloroglucinol give monomers with one, two, and three oxazine units at a single benzene ring, respectively. Aside from the synthesis and characterization of such multifunctional benzoxazines, reactivity and polymerization behavior is studied in dependence of the oxazine functionality. Monomer reactivities are directly related to the number of oxazine functionalities present at the benzene ring yielding the lowest polymerization temperature for the trifunctional phloroglucinol-based benzoxazine. Comparing the polymerization processes and resulting structures, the trifunctional benzoxazine derivative enter new polymerization pathways, which include methylene linkages bridging aniline units, as well as the formation of carbonyl-derived structures.
The invention provides certain thiazole-substituted aminoheteroaryl compounds of the Formula (I) (I), or pharmaceutically acceptable salts thereof, wherein ring R.sup.1, R.sup.2, R.sup.3, R.sup.4, ri
Shiramizu B, Theodore D, Bassett R, Coel M, Sherman KE, Glesby MJ, Chow D, Alston B, Colquhoun D, Merigan TC Jr, Reichman RC, Berggren R, Burning WJ, Brobst S; Adult AIDS Clinical Trials Group 5096 Team. Correlation of single photon emission computed tomography parameters as a noninvasive alternative to liver biopsies in assessing liver involvement in the setting of HIV and hepatitis C virus coinfection: a multicenter trial of the Adult AIDS Clinical Trials Group. J Acquir Immune Defic Syndr. 2003;33:329-335 ...
Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red stains intracellular lipid droplets yellow. In most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment can be intensely fluorescent, with varying colours from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages). The dye is highly solvatochromic and its emission and excitation wavelength both shift depending on solvent polarity [1] and in polar media will hardly fluoresce at all.[2] Nile red has applications in cell biology, where it can be used as a membrane dye which can be readily visualized using an epifluorescence microscope with excitation and emission wavelengths usually shared with red fluorescent protein. Nile red has also been used as part of a sensitive detection process for microplastics in bottled water.[3][4] ...
BioAssay record AID 465093 submitted by ChEMBL: Antibacterial activity against Mycobacterium intracellular by after 4 days alamar blue assay.
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
A list of words that start with Ox in the enable censored word list. ox oxacillin oxacillins oxalacetate oxalacetates oxalate oxalated oxalates oxalating oxalic oxalis oxalises oxaloacetate oxaloacetates oxazepam oxazepams oxazine oxazines oxblood oxbloods etc.
Supravital staining is a method of staining used in microscopy to examine living cells that have been removed from an organism. It differs from intravital staining, which is done by injecting or otherwise introducing the stain into the body. Thus a supravital stain may have a greater toxicity, as only a few cells need to survive it a short while. The term "vital stain" is used by some authors to refer specifically to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. As the cells are alive and unfixed, outside the body, supravital stains are temporary in nature. The most common supravital stain is performed on reticulocytes using new methylene blue or brilliant cresyl blue, which makes it possible to see the reticulofilamentous pattern of ribosomes characteristically precipitated in these live immature red blood cells by the supravital stains. By counting the number of such cells the rate of red blood ...
DESCRIPTION (provided by applicant): In response to NIH RFA: RM-12-014, it is proposed to engineer exosomes, the bodys "natural antigen delivery system," in order to enhance their natural capacity to activate reductive prodrugs and attach to their surface ligands that specifically target HER2-positive breast cancer, many cases of which are resistant to the current therapies (e.g., trastuzumab) . This will be done for an enzyme-activated prodrug therapy newly discovered by the P.I. (A.C. Matin, Ph.D.). The prodrug is 6-chloro-9-nitro-5-oxo-5H-benzo-(a)-phenoxazine (CNOB), and the humanized enzyme improved by the PI is hChrR43. A highly helpful feature of this regimen is that the activated cytotoxic product of CNOB, 9-amino-6-chloro-5H-benzo[a]phenoxazine-5-one (MCHB), is strongly fluorescent and can be visualized in living mice. This property makes an observational approach possible, and will minimize the need for mouse sacrifice and the use of more involved tests, e.g., LC/MS/MS, ...
Abstract Background Tuberculosis is counted amongst the most infectious and lethal illnesses worldwide and remains one of the major threats to human health. The aim of the current study was to isolate and characterize anti-mycobacterial compounds present in Curtisia dentata (Burm.f.) C.A.Sm , a medicinal plant reportedly used in the treatment of tuberculosis, stomach ailments and sexually transmitted infections. Methods The bioassay guided principle was followed to isolate the anti-mycobacterial compounds. The crude ethanol extracts of the leaves was partitioned with various solvents four compounds such as β-sitosterol, betulinic acid, ursolic acid and lupeol were successfully isolated. The compounds and their derivatives were evaluated for anti-mycobacterial activity using Microplate Alamar Blue Assay (MABA) against Mycobacterium tuberculosis H37RV (ATCC 27294). Furthermore, the derivatives were investigated for their toxicity against HepG2 and HEK293 using the MTT assay. Results The methanol ...
1 Swanson MA, Palmeri D, Vossler ED, Bartus SA, Hull D, Schweizer RT. Noncompliance in organ transplant recipients. Pharmacotherapy. 1991;11(6):173S-174S. 2 Ickovics JR, Meade CS. Adherence to antiretroviral therapy among patients with HIV: a critical link between behavioral and biomedical sciences. J Acquir Immune Defic Syndr. 2002;31(Suppl 3):S98-102. 3 Singh N, Berman SM, Swindells S, Justis JC, Mohr JA, Squier C, et al. Adherence of human immunodeficiency virus-infected patients to antiretroviral therapy. Clin Infect Dis. 1999;29(4):824-30. 4 Ickovics JR, Cameron A, Zackin R, Bassett R, Chesney M, Johnson VA, et al. Consequences and determinants of adherence to antiretroviral medication: results from Adult AIDS Clinical Trials Group protocol 370. Antivir Ther. 2002;7(3):185-93. 5 Derouin F, Pelloux H. Prevention of toxoplasmosis in transplant patients. Clin Microbiol Infect. 2008;14(12):1089-101. 6 Stein E, Ebeling P, Shane E. Post-transplantation osteoporosis. Endocrinol Metab Clin North ...
OBJECTIVES: OSKIRA-4 evaluated the efficacy of fostamatinib monotherapy versus placebo on the signs and symptoms of rheumatoid arthritis over 6 weeks by Disease Activity Score C reactive protein (DAS-28(CRP)) and assessed non-inferiority to adalimumab monotherapy at Week 24 by DAS-28(CRP). METHODS: Overall, 279 patients not currently taking disease-modifying antirheumatic drugs were randomised to: (A) fostamatinib 100 mg twice daily for 24 weeks plus placebo injection every 2 weeks (PBOI); (B) fostamatinib 100 mg twice daily for 4 weeks, then 150 mg once daily up to Week 24, plus PBOI; (C) fostamatinib 100 mg twice daily for 4 weeks, then 100 mg once daily up to Week 24, plus PBOI; (D) adalimumab 40 mg every 2 weeks for 24 weeks, plus oral placebo twice daily; or (E) oral placebo twice daily for 6 weeks, plus PBOI, then a switch to arm A or B. RESULTS: Fostamatinib demonstrated a significant improvement in DAS-28(CRP) score from baseline versus placebo at Week 6 for arms A and B, but not C. Fostamatinib
3fqe: Structural insights for design of potent spleen tyrosine kinase inhibitors from crystallographic analysis of three inhibitor complexes.
Schifitto, Giovanni; Navia, Bradford A; Yiannoutsos, Constantin T; Marra, Christina M; Chang, Linda; Ernst, Thomas; Jarvik, Jeffrey G; Miller, Eric N; Singer, Elyse J; Ellis, Ronald J; Kolson, Dennis L; Simpson, David; Nath, Avindra; Berger, Joseph; Shriver, Sharon L; Millar, Linda L; Colquhoun, Dodi; Lenkinski, Robert; Gonzalez, R Gilberto; Lipton, Stuart A; the Adult AIDS Clinical Trial Group (ACTG) 301 and 700 Teams and the HIV MRS Consortium Less ...
A compilation of outcomes of various antiretroviral therapies would be beneficial when evaluating which strategies are most effective in long-term treatment of HIV-1. Using data from present and recently completed studies, this study will collect information on therapies and their control of HIV infection and maintenance of durable suppression of HIV-1 replication.. No treatment is provided by this study, but patients will continue to receive highly active antiretroviral therapy (HAART) from other studies in which they are coenrolled. Blood and urine collection will occur at study entry and periodically throughout the study. Women may undergo pelvic exams and Pap smears. Portions of blood samples will be stored to evaluate genotypic/phenotypic susceptibility testing. Medical histories, physical exams, and questionnaires will be completed periodically. ...
A compilation of outcomes of various antiretroviral therapies would be beneficial when evaluating which strategies are most effective in long-term treatment of HIV-1. Using data from present and recently completed studies, this study will collect information on therapies and their control of HIV infection and maintenance of durable suppression of HIV-1 replication.. No treatment is provided by this study, but patients will continue to receive highly active antiretroviral therapy (HAART) from other studies in which they are coenrolled. Blood and urine collection will occur at study entry and periodically throughout the study. Women may undergo pelvic exams and Pap smears. Portions of blood samples will be stored to evaluate genotypic/phenotypic susceptibility testing. Medical histories, physical exams, and questionnaires will be completed periodically. ...
Azasetron hydrochloride 123040-16-4 NMR spectrum, Azasetron hydrochloride H-NMR spectral analysis, Azasetron hydrochloride C-NMR spectral analysis ect.
Accurate prediction of the adverse effects of test compounds on living systems, detection of toxic thresholds, and expansion of experimental data sets to include multiple toxicity end-point analysis are required for any robust screening regime. Alamar Blue is an important redox indicator that is used to evaluate metabolic function and cellular health. The Alamar Blue bioassay has been utilized over the past 50 years to assess cell viability and cytotoxicity in a range of biological and environmental systems and in a number of cell types including bacteria, yeast, fungi, protozoa and cultured mammalian and piscine cells. It offers several advantages over other metabolic indicators and other cytotoxicity assays. However, as with any bioassay, suitability must be determined for each application and cell model. This review seeks to highlight many of the important considerations involved in assay use and design in addition to the potential pitfalls.
Learn more about 3-4-dihydro-2h-1-4-benzoxazine-6-boronic-acidpinacol-ester. We enable science by offering product choice, services, process excellence and our people make it happen.
18464-44-3 - WPLZQLUEIFERRQ-UHFFFAOYSA-N - 2H-1,3-Benzoxazine-3(4H)-acetic acid, 2-oxo-, hydrazide - Similar structures search, synonyms, formulas, resource links, and other chemical information.
I am looking for someone who may be experienced in doing the Nile blue sulfate stain. I would like to talk to you or get your procedure if you have done this stain. I will be doing several and want to know what you are doing and how your results are turning out. Reply to histonet or direct email or phone. ...
3-(N-R2 -N-Acylamino)-7-(N-R3 -N-R4 -amino)-10-acylphenothiazines and phenoxazines useful as color formers, particularly in electrochromic recording systems, are prepared by the interaction of the corresponding 3-(N-R2 -amino)-7-(N-R3 -N-R4 -amino)phenothiazinium or phenoxazinium halide with a reducing agent to obtain the corresponding leuco compound and subsequently interacting the leuco compound with at least two molecular proportions of an acylating agent.
0 Journal Article %1 Neuweiler2007 %A Neuweiler, Hannes %A Lollmann, Marc %A Doose, Sören %A Sauer, Markus %C 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND %D 2007 %I ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD %J JOURNAL OF MOLECULAR BIOLOGY %N 3 %P 856-869 %T Dynamics of unfolded polypeptide chains in crowded environment studied by fluorescence correlation spectroscopy %U http://dx.doi.org/10.1016/j.jmb.2006.10.021 %V 365 %X Proteins have evolved to fold and function within a cellular environment that is characterized by high macromolecular content. The earliest step of protein folding represents intrachain contact formation of amino acid residues within an unfolded polypeptide chain. It has been proposed that macromolecular crowding can have significant effects on rates and equilibria of biomolecular processes. However, the kinetic consequences on intrachain diffusion of polypeptides, have not been tested experimentally, yet. Here, we demonstrate that selective fluorescence quenching of the oxazine ...
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Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Top performende anti-Schwein Spleen tyrosine Kinase Antikörper für Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) vergleichen & kaufen.
A new, quick method, using the resazurin dye test as a bacterial respiration indicator, has been developed to assay the antibacterial activity of various s
You are viewing an interactive 3D depiction of the molecule 9-fluoro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-2,3-dihydro-7h-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid 4-oxide (C18H10FN3O5+) from the PQR.
Indoxacarb 144171-61-9 MSDS report, Indoxacarb MSDS safety technical specifications search, Indoxacarb safety information specifications ect.
(-)-Azasetron hydrochloride | 5-HT3 antagonist | CAS [123040-96-0] - [123040-95-9] | Axon 2535 | Axon Ligand™ with >100% purity available from supplier Axon Medchem, prime source of life science reagents for your research
This study is investigating the efficacy of fostamatinib [fostamatinib disodium, R935788; Rigel Pharmaceuticals] in patients with persistent/chronic immune
BioAssay record AID 1084755 submitted by ChEMBL: Anticancer activity against Homo sapiens (human) MCF7 cells assessed as cell growth at 100 uM after 48 hr by Alamar blue assay.
Mouse monoclonal Thrombomodulin antibody [Phx-01] validated for WB, ELISA and tested in Human. Immunogen corresponding to recombinant full length protein
(±)-PHNO hydrochloride | D2 agonist | PHNO | CAS [100935-99-7] - [88058-85-9] | Axon 1070 | Axon Ligand™ with >98% purity available from supplier Axon Medchem, prime source of life science reagents for your research
RO9021 is an orally bioavailable, novel ATP-competitive inhibitor of SYK, with an average IC50 of 5.6 nM. - Mechanism of Action & Protocol.
A new drug appears to destroy lingering senescent cells, and it achieves this without using any of the previously known mechanisms or pathways.
AMPLEX S-50 Cationic anti-slip agent. Does not change the handle. Suitable to be used by padding or exhaustion. AMPLEX SIL-AN Anti-slip agent. Modifies handle. Anionic.
Information on antiretroviral dosing errors among health care providers for outpatient human immunodeficiency virus (HIV)-infected patients is lacking. We evaluated factors associated with nucleoside reverse-transcriptase inhibitor dosing errors in a university-based HIV clinic using an electronic medical record. Overall, older age, minority race or ethnicity, and didanosine use were related to such errors. Impaired renal function was more common in older patients and racial or ethnic minorities and, in conjunction with fixed-dose combination drugs, contributed to the higher rates of errors in nucleoside reverse-transcriptase inhibitor dosing. Understanding the factors related to nucleoside reverse-transcriptase inhibitor dosing errors is an important step in the building of preventive tools.
Y is an optionally substituted (C1-C4) heteroalkyl bridge that, together with the atoms to which it is attached, forms a five to seven membered heterocycle containing two to four heteroatoms selected from the group consisting of 1,3-oxazolidin-4-on-5-yl, 1,3-oxazolidin-2,4-dion-5-yl, 4,5-dihydro-1,2-oxazolidin-3-on-4-yl, 1,3-thiazolidin-4-on-5-yl, 1,3-thiazolidin-2,4-dion-5-yl, 1,3-pyrazolidin-4-on-5-yl, 1,3-imidazolidin-2,4-dion-5-yl, 1,2-pyrazolidin-3-on-4-yl, 1,2-thiazolidin-1,1,3-trion-4-yl, 1,2-thiazolidin-3-on-4-yl, tetrahydro-1,2-oxazin-3-on-4-yl, tetrahydro-1,3-oxazin-4-on-5-yl, tetrahydro-1,3-oxazin- • 2,4-dion-5-yl, morpholin-3-on-2-yl, morpholin-3,5-dion-2-yl, 2,3-dihydro-1,4-oxazin-3-on-2-yl, tetrahydro-1,3-thiazin-4-on-5-yl, tetrahydro-1,3-thiazin-2,4-dion-5-yl, tetrahydro-1,2-thiazin-3-on-4-yl, thiomorpholin-3-on-2-yl, thiomorpholin-3,5-dion-2-yl, 2,3-dihydro-1,4-thiazin-3-on-2-yl, hexahydro-1,2-diazin-3-on-4-yl, 4,5-dihydro-2H-pyridazin-3-on-4-yl, hexahydro-1,3-diazin-4-on-5-yl, ...
Alfa Aesar™ 3-Oxo-3,4-dihydro-2H-1,4-benzoxazine-6-boronic acid pinacol ester 1g Alfa Aesar™ 3-Oxo-3,4-dihydro-2H-1,4-benzoxazine-6-boronic acid...
Bioassay-guided fractionation of the chloroform extract of leaves resulted in the isolation of active antitubercular compounds alkane dotriacontane (Minimal Inhibitory Concentration-MIC 62. of its leaves extract or compounds. Plants extracts are attractive sources of new drugs and bioassay-guided fractionation is the state-of-art process to identify the active compounds contained in crude natural products. The aim of this study is to evaluate by Microplate Alamar Blue Assay (MABA) the potential antitubercular activity of leaves extracts enriched fractions and real compounds recognized by phytochemical GDC-0973 analysis. 2 Methods 2.1 Herb Material The fresh leaves of Niedenzu (IK) were collected at Estrada do Brejinho de Nazaré Tocantins State Brazil (11°01′S 48 elevation 240?m) and the species was identified by Dr Eduardo Ribeiro dos Santos of Tocantins University or college. A voucher specimen was deposited in the herbarium of the same university or college under the number 6398. 2.2 ...