Purified ovalbumin messenger RNA was employed to selectively enrich the concentration of the gene coding for ovalbumin from total chick DNA by molecular hybridization. The coding strand of the ovalbumin gene was partially purified from sheared chick DNA by affinity column chromatography using ovalbumin mRNA immobilized on phosphocellulose. The concentrations of the ovalbumin DNA sequence in various DNA fractions were quantitated by measuring their rates of hybridization with 125I-labeled ovalbumin mRNA. When apparent Cot1/2 values of these reactions were compared to the apparent Cot1/2 value obtained from the hybridization reaction between 125I-ovalbumin mRNA and complementary DNA synthesized against ovalbumin mRNA using the enzyme reverse transcriptase, purification of the coding ovalbumin DNA strand over total chick DNA was estimated to be approximately 9,600-fold. There was no apparent degradation of the 4,000 nucleotide strands of chick DNA throughout the purification procedure. Since ...
TY - JOUR. T1 - Translation of ovalbumin mRNA in Xenopus laevis oocytes. Characterization of the system and effects of estrogen on injected mRNA populations. AU - Chan, L.. AU - Kohler, Peter. AU - OMalley, B. W.. PY - 1976. Y1 - 1976. N2 - Ovalbumin messenger RNA (mRNA(ov)) purified from hen oviduct was injected into Xenopus laevis oocytes. The oocytes were incubated in culture medium containing [3H] leucine. Analysis of the oocyte cytosol on Sephadex G 150 columns demonstrated a peak of radioactivity which cochromatographed with authentic ovalbumin. Radioactive protein contained in this peak was precipitated by ovalbumin antiserum, co electrophoresed with ovalbumin on sodium dodecyl sulfate (SDS) and urea gels at pH 8.7, and eluted with the protein at the same pH (4.8) on CM cellulose chromatography. Injection of increasing amounts of mRNA(ov) was found to elicit a linear response in terms of ovalbumin synthesis. Moreover, there was linear incorporation of radioactivity into microinjected ...
Stein P.E., Leslie A.G.W., Finch J.T., Carrell R.W.. Ovalbumin, the major protein in avian egg-white, is a non-inhibitory member of the serine protease inhibitor (serpin) superfamily. The crystal structure of uncleaved, hen ovalbumin was solved by the molecular replacement method using the structure of plakalbumin, a proteolytically cleaved form of ovalbumin, as a starting model. The final refined model, including four ovalbumin molecules, 678 water molecules and a single metal ion, has a crystallographic R-factor of 17.4% for all reflections between 6.0 and 1.95 A resolution. The root-mean-square deviation from ideal values in bond lengths is 0.02 A and in bond angles is 2.9 degrees. This is the first crystal structure of a member of the serpin family in an uncleaved form. Surprisingly, the peptide that is homologous to the reactive centre of inhibitory serpins adopts an alpha-helical conformation. The implications for the mechanism of inhibition of the inhibitory members of the family is ...
A 17-amino acid tryptic peptide of chicken ovalbumin, designated P323-339, that substituted for processed antigen when presented by glutaraldehyde prefixed accessory cells to specific I-restricted T hybridomas was characterized. The peptide antigen could not be demonstrated to have any specific or stable interactions with accessory cell Ia antigens by either direct binding or functional assays for inhibition of specific T cell activation. In addition, the T cell receptor for I-restricted antigen had no affinity for free antigen alone. A rabbit antibody specific for the antigenic peptide inhibited presentation when introduced before but not after binding of the peptide to accessory cells. These results extend our earlier finding that accessory cell-mediated processing of chicken ovalbumin can be completely explained by the fragmentation of the native molecule into smaller m.w. peptides, and suggests that if an antigen/Ia complex is important in T cell activation, it forms significantly only in ...
TY - JOUR. T1 - Ovalbumin-sensitized mice are good models for airway hyperresponsiveness but not acute physiological responses to allergen inhalation. AU - Zosky, G. R.. AU - Larcombe, A. N.. AU - White, O. J.. AU - Burchell, J. T.. AU - Janosi, T. Z.. AU - Hantos, Z.. AU - Holt, P. G.. AU - Sly, P. D.. AU - Turner, D. J.. PY - 2008/5. Y1 - 2008/5. N2 - Background: Asthma is a chronic inflammatory disease that is characterized clinically by airway hyperresponsiveness (AHR) to bronchoconstricting agents. The physiological response of the asthmatic lung to inhaled allergen is often characterized by two distinct phases: an early-phase response (EPR) within the first hour following exposure that subsides and a late-phase response (LPR) that is more prolonged and may occur several hours later. Mouse models of asthma have become increasingly popular and should be designed to exhibit an EPR, LPR and AHR. Objective: To determine whether a common model of asthma is capable of demonstrating an EPR, LPR ...
Streptococcal mitogenic exotoxin Z-2 (SMEZ-2) is certainly a streptococcal superantigen that primarily stimulates human T cells bearing V8 and mouse T cells bearing V11. conjugated to ovalbumin (M1-ovalbumin) resulted in faster and quantitatively higher degrees of anti-ovalbumin IgG, Kaempferol supplier with endpoint titers getting 1,000- to 10,000-flip higher than those in pets immunized with unconjugated ovalbumin. Substantially higher degrees of anti-ovalbumin IgG had been seen in mice transgenic for individual main histocompatibility complicated (MHC) course II. Substitution of M1 with an MHC course II binding mutant (DM) removed enhanced immunity, recommending that M1 improved the delivery of antigen via MHC course II-positive antigen-presenting cells that predominate within lymphoid tissues. Immunization of pets using a conjugate comprising M1 and ovalbumin peptide from positions 323 Kaempferol supplier to 339 generated degrees of anti-peptide IgG 100-fold greater than those in pets ...
The early life period represents a time of immunological plasticity whereby the functionality immature immune system is highly susceptible to environmental stimulation. Perennial aeroallergen and respiratory viral infection induced sporadic episodes of lung inflammation during this temporal window represent major risk factors for initiation of allergic asthmatic disease. Murine models are widely used as an investigative tool to examine the pathophysiology of allergic asthma; however, models in current usage typically do not encapsulate the early life period which represents the time of maximal risk for disease inception in humans. To address this issue, this protocol adapted an experimental animal model of disease for sensitization to ovalbumin during the immediate post-weaning period beginning at 21 days of age. By initially sensitizing mice during this early life post-weaning period, researchers can more closely align experimental allergic. ...
The major goal of this study was to examine directly the capacity of Th1 cells to counterbalance the proasthmatic effects of Th2 cells in a murine model of asthma. The Th1/Th2 paradigm suggests that Th1 and Th2 cells counterbalance each other and that Th1 cells protect or prevent Th2-mediated allergic disease and asthma (27). However, using well-defined, phenotypically committed OVA-specific Th1 and Th2 cells expressing identical TCRs and adoptively transferred into either SCID mice or into OVA-immunized BALB/c mice, we found that OVA-specific Th1 cells failed to reverse Th2-mediated airway inflammation and airway hyperreactivity, even when given in twofold excess. These studies indicate that the Th1/Th2 paradigm, which predicts that Th1 cells downregulate allergic disease and asthma, may be more complex than initially appreciated and that suppression of allergic inflammation and Th2 activity in vivo may depend on cells other than Th1 lymphocytes.. Inasmuch as asthma is associated with the ...
The integrin CD103 is the αE chain of integrin αEβ7 that is important in the maintenance of intraepithelial lymphocytes and recruitment of T cells and dendritic cells (DC) to mucosal surfaces. The role of CD103 in intestinal immune homeostasis has been well described, however, its role in allergic airway inflammation is less well understood. In this study, we used an ovalbumin (OVA)-induced, CD103-knockout (KO) BALB/c mouse model of experimental allergic airways disease (EAAD) to investigate the role of CD103 in disease expression, CD4+ T-cell activation and DC activation and function in airways and lymph nodes. We found reduced airways hyper-responsiveness and eosinophil recruitment to airways after aerosol challenge of CD103 KO compared to wild-type (WT) mice, although CD103 KO mice showed enhanced serum OVA-specific IgE levels. Following aerosol challenge, total numbers of effector and regulatory CD4+ T-cell subsets were significantly increased in the airways of WT but not CD103 KO mice, ...
The primary objective of this study is to determine if broccoli sprouts (BS) improves airway inflammatory, oxidative stress (OS), and symptoms among asthmatic adults with aeroallergen sensitization.. The study is a double-blind, placebo-controlled, randomized trial to compare BS to placebo in 40 adults with asthma. 40 adults (age 18-50) who meet these eligibility criteria will be randomized to receive either: (a) BS or (b) placebo (alfalfa sprouts). Subjects will eat a sprouts sandwich daily for three days, and then undergo repeat measurement of outcomes. ...
This is the first report to suggest that in vivo inhibition of RhoA signalling may be a target for the treatment of asthma. Y-27632 significantly suppressed airway responsiveness induced by bronchospastic agonists in normal mice, allergen sensitised mice, and allergen sensitised mice infected with a virus.. Airway constriction caused by smooth muscle contraction is a critical feature of asthma. Histamine, neurokinin A, cysteinyl leukotriene, serotonin, methacholine, and acetylcholine are known bronchospastic agonists.22,23 Stimulation of their respective trimeric G protein-coupled receptors activates phospholipase C signalling pathways,24-26 leading to intracellular Ca2+ flux and smooth muscle contraction. Rho kinase inhibition can relax bronchi precontracted by histamine and neurokinin A, suggesting that RhoA activation is an integral part of the pathway leading to smooth muscle contraction.15 Methacholine and acetylcholine stimulate both muscarinic 3 (M3) and muscarinic 2 (M2) receptors.22,27 ...
Sigma-Aldrich offers abstracts and full-text articles by [Tamara Hilmenyuk, Iris Bellinghausen, Bärbel Heydenreich, Anne Ilchmann, Masako Toda, Stephan Grabbe, Joachim Saloga].
The Ova Antigen Delivery Reagent has been developed for efficient in vitro targeting of ovalbumin to antigen-presenting cells (APCs), for example, DCs. The reagent consists of a monoclonal anti-biotin antibody conjugated to ovalbumin and FITC. In combination with an appropriate biotinylated anti-receptor antibody, any desired antigen uptake receptor can be targeted.The Antigen Delivery Module Set comprises all the reagents that are required for the isolation of DCs, antigen delivery, and subsequent analysis of antigen presentation. - Österreich
Samples of lyophilized ovalbumin were irradiated, under vacuum in a high energy, uniform field of γ-rays, to absorb dosages of 30, 60 and 100 electron volts/molecule (6.5, 13.0 and 21.7 × 106 rads). Solutions of the γ-irradiated ovalbumins were fractionated by promptly precipitating the radiation-denatured molecules at the isoelectric point of native ovalbumin, and by heating the neutral systems at 50°, 60° and 68°C and by precipitating the thermolabile constituents at the isoelectric point. Quantitative serologic tests on the solutions revealed that the irradiated ovalbumins had a dose-related loss of correspondence to native protein. Removal of the radiation-denatured and thermolabile constituents improved the correspondence of the supernatants to the native solutions. Supernatants from native and irradiated preparations, heated at 68°C, showed thermal damage serologically. Radiation-denatured and thermolabile proteins exhibited a low, essentially constant and doseunrelated serologic ...
This enhanced uptake of the intravenously administered antigen was partly retarded when OVA protein was intravenously injected into Col26-7ND tumorbearing WT
ml, 1 mg, 200 µl Ovalbumin Antibody (Monoclonal, 200 µL Ovalbumin Antibody (Monoclonal, 200 µl AFP Antibody (Monoclonal, 200 µL Aprotinin Antibody (Monoclonal, 200 µL CGRP Antibody (Monoclonal
Acute exposure of sensitized mice to antigen elicits allergic airway disease (AAD) characterized by Th2 cytokine-dependent pulmonary eosinophilia, methacholine hyperresponsiveness and antigen-specific IgE elevation. However, chronic exposure induces a local inhalational tolerance (LIT), with resolution of the airway responses but persistent systemic IgE production. To further determine if systemic immunologic responses were maintained during LIT, we assessed subcutaneous late phase responses to ovalbumin in this model. Sensitized and AAD mice developed small subcutaneous responses to ovalbumin, with footpad thickness increasing to 113.7 and 113.6% of baseline, respectively. In comparison, LIT mice developed marked foot swelling (141.6%). Histologic examination confirmed increased inflammation in the chronic animals, with a significant contribution by eosinophils. Thus, the resolution of airway inflammatory responses with chronic antigen inhalation is a localized response, not associated
The present study was carried out to verify if DMPP, a nAChR agonist, could have a protective effect on the development of airway inflammation and responsiveness in a murine model of asthma, and to evaluate possible mechanisms of action. The results support the hypothesis that these agonists have a protective effect on airway inflammation and responsiveness and that this effect may be related to antigen sensitisation and calcium metabolism. Taken together, these results confirm other published data by the current authors and others 8, 9, 10, 21.. It is important to separate the specific anti-inflammatory effects of nicotinic agonists, such as nicotine, from the overall immunosuppressive effect of smoking. The present authors do not wish to suggest that cigarette smoking could be beneficial for asthmatic patients. However, there is evidence in the literature that the use of nicotinic agonists could be efficient in treating some inflammatory diseases 8, 9, 10. As nicotine crosses the blood-brain ...
Background: The deleterious effects of atmospheric pollution on human health are also known by increasing incidence and exacerbations of pre-existing lung diseases such as asthma. However, the effects of diesel exhausted particles (DEP) on acute allergic pulmonary inflammation during asthma sensitization process remain unclear. Objective: To evaluate the effects of 5 weeks of DEP exposure during the sensitization process of acute allergic pulmonary inflammation in an experimental murine model. Methods: Male Swiss mice were divided into 4 groups: non OVA-sensitized and saline instillation animals (10 µL/mouse) (Control, n=6), non OVA-sensitized and DEP instillation mice (3 mg/mL-10 µL/mouse) (DEP, n=8), OVA-sensitized and saline instillation animals (OVA, n=6) and OVA-sensitized and DEP instillation mice (DEP+OVA, n=8). 24 hours after OVA or saline aerosol challenge anesthetized mice were euthanized and was performed measures of inflammatory cells from bronchoalveolar fluid (BALF) and IL-4, ...
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Creative Biolabs provides Mouse anti-OVA T cell receptor (22.3), pCDTCR1 product for Biopharmaceutical research,preclinical and clinical trials.
TY - JOUR. T1 - Role of macrophage migration inhibitory factor in ovalbumin-induced airway inflammation in rats. AU - Kobayashi, M.. AU - Nasuhara, Y.. AU - Kamachi, A.. AU - Tanino, Y.. AU - Betsuyaku, Tomoko. AU - Yamaguchi, E.. AU - Nishihira, J.. AU - Nishimura, M.. PY - 2006/4. Y1 - 2006/4. N2 - Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that reportedly counteracts the anti-inflammatory effect of endogenous glucocorticoids. There have only been a few reports that demonstrate a potential link between MIF and bronchial asthma. In an attempt to further clarify the precise role of MIF in asthma, the present authors examined the effect of anti-MIF antibody (Ab) on airway inflammation and airway hyperresponsiveness in an ovalbumin-immunised rat asthma model. Actively immunised Brown Norway rats received ovalbumin inhalation with or without treatment of anti-MIF Ab. The levels of MIF in bronchoalveolar lavage fluid were significantly elevated after the ovalbumin ...
Chicken Egg Ovalbumin (Gal d 2) ELISA Kit, 96 tests, Quantitative ELISA Kit 6050 Chicken Egg Ovalbumin ELISA Kit Enzyme-linked immunosorbent assay (ELISA Kit); enzyme immunoassay (EIA); animal (mouse;; rat;; human;; monkey etc) crossreactivity; serum plasma and biolgoical fluids; peptide;; proteins;; ELISA;; antibody;; Chicken proteins Allergy ovalbumin turkey egg white yolk ovalbumin ova peptides autoimmune autoantibodies contaminants food allergens vaccines Chicken Egg Ovalbumin (Gal d 2) ELISA Kit, 96 tests, Quantitative ELISA Kit 6050 Chicken Egg Ovalbumin ELISA Kit Enzyme-linked immunosorbent assay (ELISA Kit); enzyme immunoassay (EIA); animal (mouse;; rat;; human;; monkey etc) crossreactivity; serum plasma and biolgoical fluids; peptide;; proteins;; ELISA;; antibody;; Chicken proteins Allergy ovalbumin turkey egg white yolk ovalbumin ova peptides autoimmune autoantibodies contaminants food allergens vaccines
TY - JOUR. T1 - Aerosol-induced immunoglobulin (Ig)-E unresponsiveness to ovalbumin does not require CD8+ or T cell receptor (TCR)-γ/δ+ T cells or interferon (IFN)-γ in a murine model of allergen sensitization. AU - Seymour, Brian W P. AU - Gershwin, Laurel J. AU - Coffman, Robert L.. PY - 1998/3/2. Y1 - 1998/3/2. N2 - Mice expressed for 20 min daily to aerosolized ovalbumin (OVA) for 10 d at concentrations from 1 to 0.01% OVA made greatly reduced immunoglobulin (Ig)-E responses to subsequent immunogenic OVA challenges, given either intraperitoneally or aerosol. This IgE-specific unresponsiveness lasted for at least four months. However, these aerosol-treated mice were primed for larger OVA-specific IgG1 and IgG2a responses. The specific reduction in IgE responses was not due to preferential induction of a T helper (Th)-1 response as aerosol OVA-primed mice made greatly reduced Th2 and no detectable Th1 response after rechallenge in vitro. Consistent with this, the increase in circulating ...
p,Mice fed the standard diet showed the induction of serum OVA-specific IgE production following oral sensitization with OVA plus CT (,xref ref-type=fig rid=pone-0044338-g001,,bold,Figure 1B,/bold,,/xref,) as previously described ,xref ref-type=bibr rid=pone.0044338-Li1,[12],/xref,. In contrast, the mice fed a standard diet plus resveratrol showed a decrease in OVA-specific IgE production after oral sensitization (,xref ref-type=fig rid=pone-0044338-g001,,bold,Figure 1B,/bold,,/xref,). Consistent with the findings of the serum IgE levels, resveratrol-fed mice sensitized with OVA plus CT showed a smaller drop in the extent of rectal temperatures than the control mice sensitized with OVA plus CT, upon intraperitoneal OVA challenge (,xref ref-type=fig rid=pone-0044338-g001,,bold,Figure 1C,/bold,,/xref,). In addition, OVA-specific IL-13 and IFN-γ productions from splenocytes and mesenteric lymph nodes (MLN)-derived cells were decreased in resveratrol-fed mice in comparison to those ...
Dai, Yun-Lei, Gui-Lian Shao, Fang Wang (2015) Extract of Brassica rapa suppresses ovalbumin-induced asthma by down-regulating NF-kB and p38 MAPK activity. [Publication] Full text not available from this repository ...
M. Al-Laith; A. Weyer; N. Havet; C. Dumarey; B. Vargaftig; M. Bachelet, 1992: Passive sensitization of guinea-pig lung mast cells and alveolar macrophages with anti-ovalbumin IgG1 and IgG2 antibodies
Cross-priming is a critical component of T cell responses to cancers and viruses, and involves transfer of antigen from antigen donor cells to the antigen presenting cells. In spite of the centrality of antigen in this process, the influence of the quantity of antigen expressed by the antigen donor cell on the efficiency of cross-priming remains unexamined. Here, I describe the creation of a novel system where the model antigen ovalbumin is expressed in P815 (d haplotype) cells under the control of an inducible promoter, producing a large amount of antigen synthesis upon induction. However, even in the un-induced condition, a very low level of ovalbumin can be detected using sensitive methods to amplify the weak signal. I have used titrated quantities of uninduced and induced cells, expressing vastly different quantities of ovalbumin, and have monitored cross-priming of the endogenous anti-OVA CD8+ T cell response quantitatively in C57BL/6 mice (b haplotype), using in vivo cytolytic T lymphocyte assays.
Cardiac antigen-specific CD8+ T cells are involved in the autoimmune component of human myocarditis. Here, we studied the differentiation and migration of pathogenic CD8+ T cell effector cells in a new mouse model of autoimmune myocarditis. A transgenic mouse line was derived that expresses cardiac myocyte restricted membrane-bound ovalbumin (CMy-mOva). The endogenous adaptive immune system of CMy-mOva mice displays tolerance to ovalbumin. Adoptive transfer of naive CD8+ T cells from the ovalbumin-specific T cell receptor-transgenic (TCR-transgenic) OT-I strain induces myocarditis in CMy-mOva mice only after subsequent inoculation with ovalbumin-expressing vesicular stomatitis virus (VSV-Ova). OT-I effector T cells derived in vitro in the presence or absence of IL-12 were adoptively transferred into CMy-mOva mice, and the consequences were compared. Although IL-12 was not required for the generation of cytolytic and IFN-γ-producing effector T cells, only effectors primed in the presence of ...
Cardiac antigen-specific CD8+ T cells are involved in the autoimmune component of human myocarditis. Here, we studied the differentiation and migration of pathogenic CD8+ T cell effector cells in a new mouse model of autoimmune myocarditis. A transgenic mouse line was derived that expresses cardiac myocyte restricted membrane-bound ovalbumin (CMy-mOva). The endogenous adaptive immune system of CMy-mOva mice displays tolerance to ovalbumin. Adoptive transfer of naive CD8+ T cells from the ovalbumin-specific T cell receptor-transgenic (TCR-transgenic) OT-I strain induces myocarditis in CMy-mOva mice only after subsequent inoculation with ovalbumin-expressing vesicular stomatitis virus (VSV-Ova). OT-I effector T cells derived in vitro in the presence or absence of IL-12 were adoptively transferred into CMy-mOva mice, and the consequences were compared. Although IL-12 was not required for the generation of cytolytic and IFN-γ-producing effector T cells, only effectors primed in the presence of ...
Asthma is a disease characterized by spontaneous contraction of the airways in response to a wide variety of endogenous and exogenous stimuli. Many asthma models are used to mimic the human asthma model in the literature. In order to better understand the role of the cannabinoid (CB) 2 receptor in the ovalbumin (OVA)-induced asthma model, a combination of both selective CB2 agonist (AM1241) and antagonist (AM630) was used to improve inflammatory hypersensitivity and edema in rats. In the present study, it was found that OVA decreased body weight (p|0.05), increased lung weights (p|0.05), increased diastolic and systolic blood pressure (p|0.001), and caused to irregularity in pulmonary functions (p|0.001). Moreover, CB2 agonist was found not to reduce body weight, cause blood pressure and respiratory irregularities (p| 0.05). OVA led to increase in IgE, TNF-α, IL-4, MDA level (p|0.001) and total WBC count (p|0.05). CB2 treatment caused to reduce the number of total WBC and the level of total protein in
Effects of respiratory viral infection on airway epithelium include airway hyper-responsiveness and inflammation. Both features may contribute to the development of asthma. Excessive damage and loss of epithelial cells are characteristic in asthma and may result from viral infection. To investigate apoptosis in Adenoviral-infected Guinea pigs and determine the role of death receptor and ligand expression in the airway epithelial response to limit viral infection. Animal models included both an Acute and a Chronic Adeno-infection with ovalbumin-induced airway inflammation with/without corticosteroid treatment. Isolated airway epithelial cells were cultured to study viral production after infection under similar conditions. Immunohistochemistry, western blots and viral DNA detection were used to assess apoptosis, death receptor and TRAIL expression and viral release. In vivo and in vitro Adeno-infection demonstrated different apoptotic and death receptors (DR) 4 and 5 expression in response to
Interleukin (IL)-4-producing-CD8 (cytotoxic T cells, Tc) contribute to lung eosinophilia and airway hyper-responsiveness (AHR) to an antigen. CD4+CD25+ regulatory T cells (Tregs) attenuate airway inflammation and AHR. This study investigated whether Tregs decrease Tc2frequencies in ovalbumin (OVA)-induced asthma model of mice. Female C57BL/6 mice were sensitized with OVA intraperitoneally and challenged with OVA intranasally to induce allergic asthma model. Tregs were sorted by fluorescence activated cell sorting (FACS) and magnetic activated cell sorting (MACS) microbeads. OVA-sensitized mice were injected with Tregs or phosphate buffer saline (PBS) by tail vein ahead of the first challenge. Airway inflammation and airway hyper-responsiveness (AHR)were evaluated by histological analysis and invasive method, respectively. OVA-specific IgE and cytokine levels were detected by ELISA. Flow cytometry was used to detect the percentages of Tc1 and Tc2. Gata3 and T-bet mRNA was determined by ...
Toxoplasma gondii is an obligate intracellular pathogen that is able to survive in a range of hosts due to its repertoire of secreted effector proteins. Utilizing the type I and type II Δku80 strains we engineered a collection of targeted knockout strains that lack a ROP or GRA protein. We determined the virulence of each strain in mice and identified type I GRA12, ROP5, ROP18 and type II ROP5 as major virulence factors. Controlling a T. gondii infection requires a strong CD8+ T cell and IFN-γ host immune response, which is activated by MHC-I antigen presentation. We engineered each of our type I knockout strains to express the model antigen ovalbumin (OVA) and tested each for their ability to modify MHC-I antigen presentation. Infection of macrophages and dendritic cells with knockout strains that lacked type I GRA2, GRA12, ROP5 or ROP18 exhibited significantly higher levels of antigen presentation than the OVA-expressing parental Δku80 strain. The ability of ROP18 to block antigen ...
Ovalbumin and lysozyme have been reported to undergo a mixed association in solutions of low ionic strength. Osmotic pressure experiments were performed on ovalbumin and on lysozyme solutions in 0.06 M cacodylate buffer (I = 0.02, pH = 5.8) at 30 and
An integrated investigative team of environmental and biomedical scientists from Michigan State University (MSU) and the Southern California Particle Center and Supersite (SCPCS) will conduct atmospheric and toxicologic research at three sites in the Los Angeles Basin (LAB), each of which have distinct air-pollution profiles. One designated site in the LAB contains locally generated PM and gaseous co-pollutants (source emission site). Another site contains transported PM and gaseous co-pollutants (receptor site). The third site is geographically located between the source and the receptor sites. The team will use a recently built, state-of-the-art mobile air research laboratory (AirCARE 1), parked at the LAB sites, to conduct inhalation toxicology studies exposing laboratory rodents with and without pre-existing allergic airway disease (animal model of human asthma) to concentrated PM (CAPs) from the local environment. Brown Norway (BN) rats with and without allergic airway disease will be ...
The prevalence of food allergy is rising in the western world. Allergen restriction is the chosen treatment in this condition, but continuous ingestion of the antigen has shown positive results in clinical trials. In a previous study, we have shown several allergic and metabolic alterations after 7 days of ovalbumin (OVA) ingestion by sensitized mice. The aim of this study was to investigate whether prolonged ingestion of antigen by sensitized mice would reverse the metabolic consequences caused by experimental food allergy. For this, allergic and metabolic parameters were analysed after prolonged ingestion of an OVA diet by OVA-sensitized mice. As shown previously, after 7 days of OVA consumption, sensitized mice showed increased serum levels of anti-OVA immunoglobulin (Ig)E and IgG1, aversion to the antigen ingestion, marked body and adipose tissue weight loss, followed by adipose tissue inflammation and decreased serum levels of adipokines, glucose and triglycerides. However, after 14 days of ...
Recently, epigenetic-mediated mechanisms - which involve heritable changes in gene expression in the absence of alterations in DNA sequences - have been proposed as contributing to asthma. In this issue of the JCI, Hollingsworth and colleagues report on the effect of prenatal maternal dietary intake of methyl donors on the risk of allergic airway disease in offspring in mice and show that these effects involve epigenetic regulation (see the related article beginning on page 3462). Supplementation of the maternal diet with methyl donors was associated with greater airway allergic inflammation and IgE production in F1 and, to some extent, F2 progeny. Site-specific differences in DNA methylation and reduced transcriptional activity were detected. If these findings are confirmed, a new paradigm for asthma pathogenesis may be emerging. ...
TY - JOUR. T1 - Non-CpG Oligonucleotides Exert Adjuvant Effects by Enhancing Cognate B Cell-T Cell Interactions, Leading to B Cell Activation, Differentiation, and Isotype Switching. AU - Herbáth, Melinda. AU - Papp, Krisztián. AU - Erdei, A.. AU - Prechl, J.. PY - 2015. Y1 - 2015. N2 - Natural and synthetic nucleic acids are known to exert immunomodulatory properties. Notably, nucleic acids are known to modulate immune function via several different pathways and various cell types, necessitating a complex interpretation of their effects. In this study we set out to compare the effects of a CpG motif containing oligodeoxynucleotide (ODN) with those of a control and an inhibitory non-CpG ODN during cognate B cell-T cell interactions. We employed an antigen presentation system using splenocytes from TCR transgenic DO11.10 mice and the ovalbumin peptide recognized by the TCR as model antigen. We followed early activation events by measuring CD69 expression, late activation by MHC class II ...
Dose-dependent specific antibody production, antigen-dependent pulmonary inflammation, and thiol changes in the lung and associated lymph nodes were examined in a Brown Norway rat model of pulmonary sensitization. Cysteine (CYSH), glutathione (GSH), and markers of inflammation in bronchoalveolar lavage fluid (BALF) were measured following ovalbumin (OVA) inhalation challenge. Alveolar macrophages
MnTE-2-PyP, a superoxide dismutase mimetic, inhibited OVA-induced airway inflammation in mice suggesting an impact in Th2 responsiveness. on immature DC. Our research claim that the main mechanism where MnTE-2-PyP inhibits airway irritation is normally by functioning on the DC and suppressing Th2 cell proliferation and activation. allergen provocation is normally elevated in asthmatics [6]. Furthermore, asthmatic sufferers demonstrate depressed degrees of endogenous antioxidant immune system such as for example superoxide dismutase (SOD) and glutathione [7]. Our lab is rolling out a SOD mimetic, MnTE-2-PyP [chemical substance name: Manganese (III) with MnTE-2-PyP demonstrated a reduced capability to support T cell proliferation, recommending an inhibitory function of MnTE-2-PyP on APC function [17]. Tse < 0.01) separate of OVA323C339 peptide concentrations when the SOD mimetic was within the culture mass media. Amount 2 MnTE-2-PyP inhibits Th2 cell proliferation. Th2 and DC cells were ...
Grapefruit seed extract (GSE) is a liquid extracted from the seeds, pulp, and white membranes of grapefruit. The herb has been used in traditional medicine as an anti bacterial, viral, fungal, parasites and to enhance immune system, protect against colds and flu and malignant disease. Grape seed extract (GSPE) may alleviate the symptoms od asthma through its antioxidant activities. According to the study of University of Ulsan College of Medicine, GSPE effectively suppressed inflammation in both acute and chronic mouse models of asthma, through reduced airway hyperresponsiveness (AHR), decreased inflammatory cells in the BAL fluid, reduced lung inflammation and and decreased IL-4, IL-5, IL-13, and eotaxin-1 expression(14) by downregulating the iNOS expression(15). Other study suggested that GSE significantly ameliorated of the pathological changes of ovalbumin-induced asthma, through inhibition of altered the lung parenchyma pathology and inflammation in ovalbumin-induced murine asthma model(16). ...
Changes in the levels of transforming growth factor (TGF)-beta cytokines or receptors observed during the progression of several inflammatory and fibrotic disorders have been used to implicate these cytokines in the pathophysiology of these diseases. Although correlative, these studies were inconclusive because they were unable to demonstrate actual continuous TGF-beta-mediated signaling in the involved tissues. We reasoned that the phosphorylation state and subcellular localization of Smad2, the intracellular effector of TGF-beta/activin-mediated signaling, could be used as a marker of active signaling mediated by these cytokines in situ. We therefore used an experimental model of ovalbumin-induced allergic airway inflammation and were able to demonstrate a dramatic increase in the numbers of bronchial epithelial, alveolar, and infiltrating inflammatory cells expressing nuclear phosphorylated Smad2 within the allergen-challenged lungs. This was accompanied by strong upregulation of the activin ...
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Mouse Monoclonal Anti-Ovalbumin Antibody (6 F11) [Alexa Fluor® 647]. Validated: ELISA. Tested Reactivity: Chicken. 100% Guaranteed.
November 2014. Attenuation of allergic airways inflammation by an extract of Hymenocardia acida Tracheal hyperresponsiveness, airway mucus production and bronchoalveolar inflammation are the major components of asthma. Here, we aim to investigate the role in the control of asthma of a bioactive plant extracted from Hymenocardia acida in a physiological and pathophysiological model. The effect of H. acida crude extract (HACE) on total cellular components of bronchoalveolar (BAL) fluids was performed on ovalbumin.... Author(s): Fatou Bintou Sar, Mamadou Sarr, Mama S.Y. Diallo, Saliou Ngom, Lamine Gueye, Abdoulaye Samb, Ramaroson Andriantsitohaina and Annelise Lobstein ...
Principal Investigator:OBOKI Keisuke, Project Period (FY):2010 - 2011, Research Category:Grant-in-Aid for Young Scientists (B), Research Field:膠原病・アレルギー・感染症内科学
Thus we questioned whether IL-4-producing CD8 T cells would be induced in vivo in response to primary immunization with alum-precipitated protein. The approach has been to compare the polarization of transgenic naïve ovalbumin-specific CD4 (OTII) and CD8 (OTI) T cells during their response to alum-precipitated ovalbumin (alumOVA). By addressing this question we have obtained further insight into the way early Th2-features are acquired by CD4 T cells in vivo in response to alum-precipitated protein.. Although both CD4 and CD8 OVA-specific T cells proliferate (30) in response to alumOVA, the acquisition of Th2-features, such as IL-4 and IL-13 mRNA up-regulation, is exclusively confined to CD4 T cells (Fig. 2). In addition, we confirm that mRNA specific for IL-17RB is strongly induced in vivo in alumOVA-responding OTII cells, but not in OTI cells responding to the same antigen (Fig. 2).. Conclusions. These findings indicate that the induction of IL-17RB expression is a selective feature of CD4 T ...
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TxCell (Valbonne, France) aims to treat severe chronic inflammatory and autoimmune diseases by using Tregs to tamp down the immune response. TxCell is developing therapeutics based on isolating Tregs from a patients blood, expanding them in the lab in response to a specific activating antigen, and infusing them back into the patient. Although the process is expensive, the extracted material can be frozen and used as needed, providing enough material for five years worth of treatment every few months.. Their most advanced clinical-stage product is Ova-Treg, which is in development for Crohns disease. Tregs are exposed to the protein ovalbumin, which makes up about 60% of egg whites. The Tregs recognizing ovalbumin are expanded and infused back into the patients body. Tregs naturally migrate to sites of major inflammation-or in the case of Crohns disease, the gut. Although ovalbumin is not related to the pathology of Crohns, it is typically present in the gut, so the ovalbumin-sensitive ...