Detect osteopontin protein with anti-osteopontin antibody products and ELISA kits for osteopontin test methods. Learn more about osteopontin protein.

Detect osteopontin protein with anti-osteopontin antibody products and ELISA kits for osteopontin test methods. Learn more about osteopontin protein.

R&D Systems™ Bovine Osteopontin/OPN Protein 50ug R&D Systems™ Bovine Osteopontin/OPN Protein Proteins O

OPN (osteopontin), a pro-inflammatory cytokine, has recently emerged as a key factor in both vascular remodelling and the development of atherosclerosis. However, the relationship between OPN and atherosclerosis in patients without symptomatic cardiovascular disease is not clear. Therefore we measured plasma OPN levels and evaluated the correlation between plasma OPN levels and atherosclerosis as target organ damage in patients with EHT (essential hypertension). Plasma OPN levels were measured in 76 patients with EHT using a solid-phase sandwich ELISA. IMT (intima-media thickness), and Vd and Vs (mean diastolic and systolic flow velocities respectively) were evaluated by carotid ultrasound. The Vd/Vs ratio, an index of peripheral arterial resistance, was also calculated. The patients were divided on the basis of median OPN levels into a high-OPN group and a low-OPN group. The mean IMT and aldosterone levels were higher (P=0.024 and 0.031 respectively) and Vd/Vs was lower (P=0.007) in the ...

Background: Osteopontin is a secreted phosphorylated glycoprotein that is expressed by a variety of cell types and that mediates numerous and diverse biological functions. ...

The design and selection criteria of the Framingham Offspring study have been described previously.2 On the basis of the sex-specific distributions of echocardiographic LV measurements, we sampled participants from the sixth examination cycle (1995-8) with both LV end diastolic dimension (LVEDD) and wall thickness (LVWT) below the sex-specific median (referent, n = 129), with increased LVEDD (⩾ 90th sex-specific centile, n = 134) and increased LVWT (⩾ 90th sex-specific centile, n = 128) in a 1:1:1 ratio. Eighteen participants were included in both LV dilatation and increased LVWT groups, so the study sample consisted of 373 participants. Participants underwent a standardised medical history and physical examination. Fasting blood samples were drawn and frozen at −70°C without any freeze-thaw cycles until assay. Plasma osteopontin was measured in duplicate by using an enzyme-linked immunosorbent assay (Calbiochem, Inc) with an intra-assay coefficient of variation of 4.6 ...

In a previous study, we demonstrated that nucleotides induce an increase in OPN mRNA steady-state levels in SMCs cultured in the presence or absence of serum.6 The present work shows that the UTP-induced OPN mRNA increase in rat SMCs results from both OPN mRNA stabilization and OPN gene transcription.. To date, few studies have tried to establish the mechanisms of OPN mRNA increase. In particular, it has been shown that neither transforming growth factor-β, a growth factor activating serine/threonine kinase receptors, nor intracellular receptor agonists, such as glucocorticoids and retinoic acid,37,38 induce OPN mRNA stability.3 In this study, we demonstrate that OPN mRNA level is regulated by UTP via a mRNA stabilization mechanism. Ang II, another agonist of G-protein-coupled receptors, can also stabilize OPN mRNA but not PDGF (data not shown). The sequence analysis of OPN mRNA reveals one potential sequence involved in mRNA stability in the 3′ UTR, TAAAT (located at 1084 to 1088). However, ...

Osteopontin (OPN) is a multifunctional matricellular protein produced by a broad range of cells including osteoclasts, macrophages, T cells, endothelial cells, and vascular smooth muscle cells. OPN modulates various physiological and pathological events such as inflammation, wound healing, and bone formation and remodeling. Dengue virus (DENV) infection causes an increase in plasma OPN levels, which is correlated with the severity of symptoms and coagulation abnormalities. DENV infection also induces OPN gene expression in human macrophages. This study investigated the inhibitory effects of brefelamide and its methyl ether derivative on DENV-3 by measuring changes in OPN levels in human THP-1 and 293T cell lines infected at different multiplicities of infection and post-infection time points. OPN mRNA expression and viral RNA were detected by reverse transcriptase quantitative real-time PCR, whereas protein level was determined by enzyme-linked immunosorbent assay. We found that viral copy number was

TY - JOUR. T1 - Osteopontin expression detected in adult cochleæ and inner ear fluids. AU - Lopez, Cecilia A.. AU - Olson, Elizabeth S.. AU - Adams, Joe C.. AU - Mou, Kewa. AU - Denhardt, David T.. AU - Davis, Robin. PY - 1995/5. Y1 - 1995/5. N2 - Localization of protein epitopes and mRNA expression showed that there was a wide-spread distribution of osteopontin (OPN) within the membranous labyrinth of the adult mammalian cochleæ. Immunoreaction product and mRNA were found within the stria vascularis, VIIIth cranial nerve, spiral ligament and limbus. Only specific cell types within these regions contained abundant OPN mRNA or protein, the main cell type being fibrocytes that populate the spiral limbus and spiral ligament. Epithelial cells that line the luminal surface of the stria vascularis (marginal cells) and neurons that compose the vestibular and auditory ganglia also showed high opn expression. The pattern of anti-OPN staining within the membranous labyrinth was comparable to that ...

TY - JOUR. T1 - Prognostic significance of osteopontin expression in human prostate cancer. AU - Forootan, Shiva S. AU - Foster, Christopher S. AU - Aachi, Vijay R. AU - Adamson, Janet. AU - Smith, Paul H. AU - Lin, Ke. AU - Ke, Youqiang. N1 - 2005 Wiley-Liss, Inc.. PY - 2006/5/1. Y1 - 2006/5/1. N2 - To test the hypothesis that expression of osteopontin (OPN), an integrin-binding glycoprotein, can independently predict the potential aggressiveness of prostate cancer, the status of OPN expression in benign and malignant prostate cancer cell lines and tissues was analysed by Western blot and immunohistochemistry. Amongst the four prostate cell lines analysed, the level of OPN expressed in the benign PNT-2 cells was set at 1, the relative level of OPN expressed in the weakly malignant cell line LNCaP was increased to 1.5. In the highly malignant cell lines Du-145 and PC-3, the level of OPN expression was further increased to 2.9 and 4.4, respectively. An increased expression of OPN was also ...

Embryo implantation is a highly synchronized bioprocess between an activated blastocyst and a receptive uterus. In mice, successful implantation relies on the dynamic interplay of estrogen and progesterone; however, the key mediators downstream of these hormones that act on blastocyst competency and endometrium receptivity acquisition are largely unknown. In this study, we showed that the expression of osteopontin (OPN) in mouse blastocysts is regulated by ovarian estrogen and uterine micro-environment. OPN mRNA is up-regulated in mouse blastocyst on day 4 of pregnancy, which is associated with ovarian estrogen secretion peak. Hormone treatment in vivo demonstrated that OPN expression in a blastocyst is regulated by estrogen through an estrogen receptor (ER). Our results of the delayed and activated implantation model showed that OPN expression is induced after estrogen injection. While estrogen treatment during embryo culture in vitro showed less effect on OPN expression, the tubal ligation model on

We initially examined whether heparin binds to OPN. The binding of biotinylated heparin to the full-length form (OPN full/glutathione S-transferase [GST]), the amino-terminal half of thrombin-cleaved form (OPN N half/GST), and the carboxy-terminal half of thrombin-cleaved form (OPN C half/GST) of human OPN was tested. Heparin bound to Escherichia coli-derived OPN full/GST (Fig. 1 A). In contrast, the same heparin preparation failed to bind to both OPN N half/GST and OPN C half/GST. OPN is heavily glycosylated in vivo (17) and E. coli-derived OPN lacks glycosylation. To exclude that the lack of sugar moiety on OPN affects binding of OPN to heparin, we prepared the glycosylated form of recombinant OPN from Chinese hamster ovary (CHO) cells. Again, heparin bound only to the full-length form of OPN in a dose-dependent manner (Fig. S2, available at http://www.jem.org/cgi/content/full/jem.20071324/DC1). Note that the amino acid sequences that correspond to two putative HBDs are 165YGLRSKSKKF174, which ...

Quantikine® ELISA kit for human Osteopontin/OPN (Cat#SOST00). 0.024 ng/mL detection sensitivity. View Osteopontin/OPN ELISA kit details.

Schistosomiasis is a major cause of fibrosis and portal hypertension. The reason 4-10% of infected subjects develops hepatosplenic schistosomiasis remains unclear. Chronically infected male CBA/J mice reproduce the dichotomic forms of human schistosomiasis. Most mice (80%) develop moderate splenomegaly syndrome (similar to hepatointestinal disease in humans) and 20% present severe hypersplenomegaly syndrome (analogous to human hepatosplenic disease). We demonstrated that the profibrogenic molecule osteopontin discriminates between mice with severe and mild disease and could be a novel morbidity biomarker in murine and human schistosomiasis. Failure to downregulate osteopontin during the chronic phase may explain why hepatosplenic subjects develop severe fibrosis ...

OPN (osteopontin)) is a Hh (Hedgehog)-regulated cytokine that is up-regulated during chronic liver injury and directly promotes fibrosis. We have reported that Hh signalling enhances viral permissiveness and replication in HCV (hepatitis C virus)-infected cells. Hence we hypothesized that OPN directly promotes HCV replication, and that targeting OPN could be beneficial in HCV. In the present study, we compared the expression of OPN mRNA and protein in HCV (JFH1)-infected Huh7 and Huh7.5 cells, and evaluated whether modulating OPN levels using exogenous OPN ligands (up-regulate OPN) or OPN-specific RNA-aptamers (neutralize OPN) leads to changes in HCV expression. Sera and livers from patients with chronic HCV were analysed to determine whether OPN levels were associated with disease severity or response to therapy. Compared with Huh7 cells, Huh7.5 cells support higher levels of HCV replication (15-fold) and expressed significantly more OPN mRNA (30-fold) and protein. Treating Huh7 cells with OPN ...

Osteopontin (OPN) is abundantly expressed in human calcified arteries. To examine the role of OPN in vascular calcification, OPN mutant mice were crossed with m

Since its first description more than 20 years ago osteopontin has emerged as an active player in many physiological and pathological processes, including biomineralization, tissue remodeling and inflammation. As an extracellular matrix protein and proinflammatory cytokine osteopontin is thought to facilitate the recruitment of monocytes/macrophages and to mediate cytokine secretion in leukocytes. Modulation of immune cell response by osteopontin has been associated with various inflammatory diseases and may play a pivotal role in the development of adipose tissue inflammation and insulin resistance. Here we summarize recent findings on the role of osteopontin in metabolic disorders, particularly focusing on diabetes and obesity.

In this study, we demonstrate for the first time that changes in blood glucose levels are readily detected by NFATc3 in arterial smooth muscle in vivo and that the calcineurin/NFATc3 signaling pathway regulates the expression of OPN, which is a key player in the development of vascular disease. Our major findings are as follows: (1) changes in extracellular glucose activate NFATc3 signaling in vivo; (2) NFATc3 has the ability to bind to at least one NFAT binding site (site 3) in the mouse promoter region of OPN; (3) NFATc3 activation leads to increased OPN mRNA and protein expression in intact arteries; (4) glucose-induced OPN expression is dependent on the release of extracellular nucleotides acting on P2Y membrane receptors; (5) glucose-induced OPN expression is prevented by pharmacological inhibition of calcineurin/NFAT signaling; and (6) STZ-induced diabetes increases OPN expression in the ascending and thoracic aorta, vascular segments particularly prone to atherosclerosis in diabetic ...

article{9ffbdf28-43ac-4664-bbde-373cd7e125c5, abstract = {The use of cancer biomarkers to anticipate the outlines of disease has been an emerging issue, especially as cancer treatment has made such positive steps in the last few years. Progress in the development of consistent malignancy markers is imminent because advances in genomics and bioinformatics have allowed the examination of immense amounts of data. Osteopontin is a phosphorylated glycoprotein secreted by activated macrophages, leukocytes, and activated T lymphocytes, and is present in extracellular fluids, at sites of inflammation, and in the extracellular matrix of mineralized tissues. Several physiologic roles have been attributed to osteopontin, i.e., in inflammation and immune function, in mineralized tissues, in vascular tissue, and in kidney. Osteopontin interacts with a variety of cell surface receptors, including several integrins and CD44. Binding of osteopontin to these cell surface receptors stimulates cell adhesion, ...

Osteopontin has long been implicated in the process of carcinogenesis, progression, and metastatic dissemination of several human tumors, such as breast (12, 13), prostate (15), colon (16), ovarian (17), gastric (18), and lung (14) cancers and more recently in many other tumors, such as pancreatic, renal, endometrial, esophageal, and head and neck carcinomas (30).. In the present study, we decided to focus on osteopontin protein expression detected by immunohistochemistry in a large sample (207 cases) of NSCLCs, because these tumors still have a poor prognosis in spite of the notable advances in diagnosis, staging, treatment, and biological characterization. We analyzed the correlations between osteopontin expression and many clinicopathologic variables to clarify its possible prognostic role. As far as we know, our study is the largest retrospective analysis of the prognostic role of osteopontin expression in patients with NSCLC treated with curative surgery. Moreover, we decided to give ...

Background The pleiotropic cytokine osteopontin (OPN) is thought to be involved in the pathogenesis of atherosclerosis. However, the relationship between OPN and renal function, a cardiovascular risk factor itself, is not known. Therefore, we assessed the relationship between OPN plasma levels and renal function in patients at different stages of chronic kidney disease (CKD).. Methods We studied 49 non-diabetic and non-smoking patients with primary kidney disease at different CKD stages (K/DOQI 1-5). True glomerular filtration rate (GFR) in patients was assessed using the inulin-clearance technique. To examine the role of an abrupt change in GFR on circulating OPN, 15 living related kidney donors were studied before and after unilateral nephrectomy. Twenty matched non-smoking healthy subjects served as controls.. Results OPN plasma levels in patients with CKD stage 1 (i.e. GFR above 90 mL min−1 1·73 m−2) were comparable with controls. OPN levels increase in a linear fashion with declining ...

3.0.CO;2-Y. PMID 10825241. Suzuki K, Zhu B, Rittling SR, Denhardt DT, Goldberg HA, McCulloch CA, Sodek J (August 2002). Colocalization of intracellular osteopontin with CD44 is associated with migration, cell fusion, and resorption in osteoclasts. J Bone Miner Res. 17 (1): 1486-1497. doi:10.1359/jbmr.2002.17.8.1486. PMID 12162503. Zhu B, Suzuki K, Goldberg HA, Rittling SR, Denhardt DT, McCulloch CA, Sodek J (January 2004). Osteopontin modulates CD44-dependent chemotaxis of peritoneal macrophages through G-protein-coupled receptors: evidence of a role for an intracellular form of osteopontin. Journal of Cellular Physiology. 198 (1): 155-167. doi:10.1002/jcp.10394. PMID 14584055. Junaid A, Moon MC, Harding GE, Zahradka P (February 2007). Osteopontin localizes to the nucleus of 293 cells and associates with polo-like kinase-1. Am J Physiol Cell Physiol. 292 (1): 919-926. doi:10.1152/ajpcell.00477.2006. PMID 17005603. Shinohara ML, Kim HJ, Kim JH, Garcia VA, Cantor H (May 2008). Alternative ...

With a multi-disciplinary approach, Fact.MR elaborates an extensive analysis of the historical, current and future outlook of the global Osteopontin market as well as the factors responsible for such a growth. Our highly dedicated professionals have inputted critical and accurate insights associated with every industry, and region by doing thorough primary and secondary research.. Request TOC of this Report- https://www.factmr.com/connectus/sample?flag=T&rep_id=3827 We leverage space-age industrial and digitalization tools to provide avant-garde actionable insights to our clients regarding the Osteopontin market. For enhancing readers experience, the report starts with a basic overview about the Osteopontin and its classification. Further, we have considered 2018 as the base year, 2019 as the estimated year, 2019 - 2029 as the stipulated timeframe.. Competitive Assessment. The Osteopontin market report includes global as well as emerging players:. ...

Osteopontin (OPN) is a highly posttranslationally modified protein present in several tissues where it is implicated in numerous physiological processes. OPN primarily exerts its functions through interaction with integrins via the Arg-Gly-Asp and Ser-Val-Val-Tyr-Gly-Leu-Arg sequences located in the N-terminal part of the protein. OPN can be polymerized by the cross-linking enzyme transglutaminase 2 (TG2), and polymerization has been shown to enhance the biological activity of OPN. However, little is known about the reactivity and location of the glutamine and lysine residues involved in the TG2-mediated modification of OPN. Here we show that TG2 catalyses the incorporation of 5-(Biotinamido)pentylamine at glutamines in both the N- and C-terminal parts of OPN, whereas TG2 primarily incorporated the glutamine-donor peptide biotinyl-TVQQEL-OH into the C-terminal part of OPN. By mass spectrometric analyses we identified Gln34, Gln42, Gln193 and Gln248 as the major TG2 reactive glutamines in OPN. The

Osteopontin represents a multifunctional molecule playing a pivotal role in chronic inflammatory and autoimmune diseases. Its expression is increased in inflammatory bowel disease (IBD). The aim of our study was to analyze the association of osteopontin (OPN/SPP1) gene variants in a large cohort of IBD patients. Genomic DNA from 2819 Caucasian individuals (n = 841 patients with Crohns disease (CD), n = 473 patients with ulcerative colitis (UC), and n = 1505 healthy unrelated controls) was analyzed for nine OPN SNPs (rs2728127, rs2853744, rs11730582, rs11739060, rs28357094, rs4754 = p.Asp80Asp, rs1126616 = p.Ala236Ala, rs1126772 and rs9138). Considering the important role of osteopontin in Th17-mediated diseases, we performed analysis for epistasis with IBD-associated IL23R variants and analyzed serum levels of the Th17 cytokine IL-22. For four OPN SNPs (rs4754, rs1126616, rs1126772 and rs9138), we observed significantly different distributions between male and female CD patients. rs4754 was ...

This study retrospectively evaluated the immunohistochemical expression of 3 cell adhesion molecules (CAMs), E-cadherin, beta-catenin, and osteopontin, according to tumor grade in 125 surgically resected specimens of hepatocellular carcinoma (HCC). The aims of this study were to identify factors associated with vascular invasion and to elucidate the prognostic value of CAMs. The median follow-up time was 110 months. The levels of E-cadherin, beta-catenin, and osteopontin immunoreactivity were significantly associated with Edmondson-Steiner grade but not with tumor size. There was increased loss of E-cadherin, nonnuclear overexpression of beta-catenin, and overexpression of osteopontin in tumors of higher histologic grade. Vascular invasion was found in 44 (35%) of 125 resected specimens. Logistic regression analysis identified 3 tumor-related factors that were independently associated with vascular invasion-tumor size more than 3 cm, Edmondson-Steiner grades III to IV, and overexpression of ...

Chronic degenerative disease of the mares endometrium is characterised by changes in the uterine glands, including cystic dilation, hyperplasia and periglandular fibrosis. Endometrial biopsies were taken from 23 mares with different grades of endometrial degeneration. Solid structures were identified within the lumina of the uterine glands and shown to be calcified by histochemical staining. Most of them were not homogenous but composed of a mixture of mineral and organic substances. Further examinations of these mineralised structures by immunohistochemical methods revealed the presence of the non-collagenous matrix proteins osteopontin, osteonectin and bone sialoprotein, which are known to be involved in calcification processes such as urolithiosis. Osteopontin and bone sialoprotein were identified within the calculi, frequently arranged in concentric layers. Osteonectin was the only matrix protein that was also present in the glandular epithelium. Osteocalcin was not found in either the ...

Our results demonstrate that incubating cultured mouse hepatocytes with MCD medium causes cellular steatosis and increased release of ALT. The MCD medium-induced increases in triglyceride formation and ALT release were accompanied by the activation of PI3-kinase and markedly increased OPN expression. An inhibitor of PI3-kinase prevented the increases in OPN expression, cellular steatosis, and ALT release. Antibodies to OPN or its receptor blunted the increase in ALT release but had no effect on steatosis induced by the MCD medium. Together, these findings indicate that the activation of PI3-kinase plays an important role in the development of hepatocyte steatosis and induction of OPN expression in cultured hepatocytes exposed to MCD medium. OPN, in turn, is involved in signaling increased ALT release by hepatocytes. As has been shown elsewhere (30), OPN expression is involved in fibrogenesis in the MCD diet model of NASH. OPN, therefore, is potentially involved in progression from steatosis to ...

Osteopontin (OPN) is a phosphorylated glycoprotein possessing an arginine-glycine-aspartate (RGD)-motif, which binds to several cell surface integrins and mediates a wide range of cellular processes. Inductions of OPN have been reported in the postischemic brain, and the neuroprotective effects of OPN have been demonstrated in animal models of stroke. In the present study, we showed a robust neuroprotective effect of RGD-containing icosamer OPN peptide (OPNpt20) in a rat model of focal cerebral ischemia (middle cerebral artery occlusion, MCAO). Intranasally administered OPNpt20 reduced mean infarct volume by 79.7 % compared to the treatment-na , ve MCAO control animals and markedly ameliorated neurological deficits. In addition, OPNpt20 significantly suppressed the inductions of iNOS and of inflammatory markers in postischemic brains and in primary microglial cultures, demonstrating anti-inflammatory effects. Administration of a mutant peptide, in which RGD was replaced by ...

TY - JOUR. T1 - Gene Expression Profiling of Paraffin-Embedded Primary Melanoma Using the DASL Assay Identifies Increased Osteopontin Expression as Predictive of Reduced Relapse-Free Survival. AU - Conway, C.. AU - Mitra, A.. AU - Jewell, R.. AU - Randerson-Moor, J.. AU - Lobo, S.. AU - Nsengimana, J.. AU - Edward, S.. AU - Sanders, D. S.. AU - Cook, M.. AU - Powell, B.. AU - Boon, A.. AU - Elliott, F.. AU - de Kort, F.. AU - Knowles, M. A.. AU - Bishop, D. T.. AU - Newton-Bishop, J.. PY - 2009. Y1 - 2009. U2 - 10.1158/1078-0432.CCR-09-1631. DO - 10.1158/1078-0432.CCR-09-1631. M3 - Article. VL - 15. SP - 6939. JO - Clinical Cancer Research. JF - Clinical Cancer Research. SN - 1078-0432. IS - 22. ER - ...

Despite impressive advances in the therapeutic approches, the long-term survival rate of acute myeloid leukemia (AML) is considered to be significantly low as a result of resistance to the treatment and desease relapse. Among multitude oncogenic proteins invovlved in aquisition of chemo-resistance phenotype, osteopontin (OPN) recently attracted tremendous attentions. In spite of the well-defined association between OPN expression and cure rate in solid tumors, there is a scarcity of analysis on the role of this protein in AML. Based on the critical role of OPN in cell survival, it seems reasonable to hypothesize that the high expression of OPN isoforms may participate in disrupting the regulation of apoptosis in AML cells and it s relapse. To investigate the association between induction of apoptosis and OPN isoform expression, two distinct AML cell lines (KG-1 as a leukemic stem cell model and U937) were treated with chemotherapy drugs and the cell viability and apoptosis were evaluated by MTT and

Anti-Osteopontin, N-terminal Antibody - MAB193P - Maine Biotechnology Services, Osteopontin Antibodies available at 207-797-5454 or [email protected]

Anti-Osteopontin, C-terminal Antibody - MAB197P - Maine Biotechnology Services, Osteopontin antibodies available at 207-797-5454 or [email protected]

OPN (osteopontin) is a multiphosphorylated extracellular glycoprotein, which has important roles in bone remodelling, inflammation and cancer metastasis. OPN regulates cell spreading and adhesion primarily through its association with several integrins such as αvβ3, and its phosphorylation affects these processes. However, the mechanism by which OPN O-glycosylation affects these processes is not completely understood. In the present study, we demonstrated that OPN O-glycosylation self-regulates its biological activities and also affects its phosphorylation status. We prepared two recombinant OPNs, WT (wild-type)-OPN and mutant OPN (ΔO-OPN), which lacks five O-glycosylation sites at a threonine/proline-rich region. O-glycan defects in OPN increased its phosphorylation level, as observed by dephosphorylation assays. Moreover, compared with WT-OPN, ΔO-OPN exhibited enhanced cell spreading and adhesion activities and decreased associations with β1 integrins. This suggested that defects in ...

An excess of OPN is associated with increased LOX and insoluble collagen, as well as with LV stiffness and systolic dysfunction in patients with HHD and HF. In addition, OPN up-regulates LOX in human fibroblasts. It is suggested that the OPN-LOX axis might facilitate the formation of insoluble colla …

Epithelio-mesenchymal interactions during kidney organogenesis are disrupted in integrin alpha8 beta1-deficient mice. However, the known ligands for integrin alpha8 beta1-fibronectin, vitronectin, and tenascin-C-are not appropriately localized to mediate all alpha8 beta1 functions in the kidney. Using a method of general utility for determining the distribution of unknown integrin ligands in situ and biochemical characterization of these ligands, we identified osteopontin (OPN) as a ligand for alpha8 beta1. We have coexpressed the extracellular domains of the mouse alpha8 and beta1 integrin subunits as a soluble heterodimer with one subunit fused to alkaline phosphatase (AP) and have used the alpha8 beta1-AP chimera as a histochemical reagent on sections of mouse embryos. Ligand localization with alpha8 beta1-AP in developing bone and kidney was observed to be overlapping with the distribution of OPN. In far Western blots of mouse embryonic protein extracts, bands were detected with sizes ...

Recombinant wild-type rabbit osteopontin (rOP) and the protein with an aspartate-to-glutamate transposition induced by a point mutation in the rabbit OP cDNA within the Gly-Arg-Gly-Asp-Ser (GRGDS) sequence were expressed in Escherichia coli and purified to homogeneity. P388D1 cells bound rOP in a saturable manner. rOP induced adhesion and haptotaxis of P388D1 cells, whereas mutated rabbit OP (rOPmut) did not. Anti-rOP IgG F(ab′)2 and synthetic GRGDS peptide inhibited rOP-mediated adhesion and haptotaxis of P388D1 cells. Fibronectin (FN)-mediated adhesion of P388D1 cells was markedly inhibited in the presence of fluid-phase rOP. Adhesion of P388D1 cells to rOP was significantly inhibited by anti-[alpha-subunits of VLA4 (alpha 4) and VLA5 (alpha 5)] monoclonal antibodies (mAbs), but not by anti-[alpha-subunit of vitronectin (VN) receptor (alpha V) or Mac-1 (alpha M)] mAb. Adhesion of P388D1 cells to FN and VN was significantly inhibited by anti-alpha V mAb but not anti-alpha 4, -alpha 5 or ...

Purpose: Gene expression studies in melanoma have been few because tumors are small and cryopreservation is rarely possible. The purpose of this study was to evaluate the Illumina DASL Array Human Cancer Panel for gene expression studies in formalin-fixed melanoma primary tumors and to identify prognostic biomarkers.. Experimental Design: Primary tumors from two studies were sampled using a tissue microarray needle. Study 1: 254 tumors from a melanoma cohort recruited from 2000 to 2006. Study 2: 218 tumors from a case-control study of patients undergoing sentinel node biopsy.. Results: RNA was obtained from 76% of blocks; 1.4% of samples failed analysis (transcripts from ,250 of the 502 genes on the DASL chip detected). Increasing age of the block and increased melanin in the tumor were associated with reduced number of genes detected. The gene whose expression was most differentially expressed in association with relapse-free survival in study 1 was osteopontin (SPP1; P = 2.11 × 10−6) and ...

1468 Hepatocellular carcinoma (HCC) exhibits a unique feature of high frequency of allelic loss at chromosome 4q. Using mRNA differential display, we identified frequent overexpression of alpha-fetoprotein (AFP) at 4q11-q13 and osteopontin (OPN) at 4q21-q25, and downregulation of annexin A10 (ANXA10) at 4q33. This study is to elucidate the clinicopathological significance of the accumulation of the aberrant expressions of these three genes in the progression of HCC using reverse transcriptase polymerase chain reaction (RT-PCR) analyses and radioimmunoassay for AFP. We showed that high AFP levels (,200 ng/ml), OPN overexpression and ANXA10 downregulation had close association with more frequent large HCC (P=0.0002, P=0.0013, and P=0.007, respectively), grade II to IV (P=0.0000, P=0.0001, and P=0.0000, respectively), and portal vein invasion (stage IIIB and IV) (P=0.0000, P=0.0000, and P=0.0001, respectively). Besides, high AFP levels correlated with OPN overexpression (P=0.0001) and ANXA10 ...

Fingerprint Dive into the research topics of Expression of osteopontin and CDX2: Indications of phenotypes and prognosis in advanced gastric cancer. Together they form a unique fingerprint. ...

Shop a large selection of products and learn more about Thermo Scientific Lab Vision Osteopontin, Rabbit Polyclonal Antibody:: 1mL; 200µg/mL; Unlabeled; Purified with

购买我们的Osteopontin肽 (288-299) (Carboxyterminal end). ab45753可作为ab36125的封闭肽并经过Blocking实验验证。Abcam提供免费的实验方案，操作技巧及专业的支持。

Christensen B, et al. (2005) Post-translationally modified residues of native human osteopontin are located in clusters: identification of 36 phosphorylation and five O-glycosylation sites and their biological implications. Biochem J 390, 285-92 ...

Mouse Monoclonal Anti-Osteopontin/OPN Antibody (1B20) [FITC]. Validated: ICC/IF, IHC-Fr, IHC-P. Tested Reactivity: Human, Rat, Rabbit. 100% Guaranteed.

Corning® BioCoat - PureCoat plastics Corning® ULA- Ultra low attachment microplates Nunclon Sphera coated plastics Greiner Bio-One Cellcoat plastics Human osteopontin coated plastics Extra-cellular matrice coated plastics Primaria coated plastics ...

Conference (2015, September 10). Glioblastoma (GBM) is the most aggressive and common solid human brain tumor. Because of GBM heterogeneity, location and aggressiveness, none of the available treatment is curative. These treatments ... [more ▼]. Glioblastoma (GBM) is the most aggressive and common solid human brain tumor. Because of GBM heterogeneity, location and aggressiveness, none of the available treatment is curative. These treatments include maximal surgical resection, radiotherapy and concomitant or adjuvant chemotherapy with Temozolomide (TMZ). However, the prognosis of adult patients with GBM remains poor and the survival outcome after treatment does not exceed 15 months. Glioblastoma-composing cells have developed many strategies to counteract these current therapies. Among the wide hallmarks acquired to survive, osteopontin (OPN) ranks correlates with lower overall and disease-free/relapse-free survival in all tumors combined, as well in brain cancer. OPN expression is largely ...

PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Extracellular peptides are most likely involved in the regulation of hematopoietic stem cell (HSC) function in the bone marrow (BM) and might be dysregulated in aberrant hematopoiesis and development of cancers. In support of this, preliminary results demonstrate (I) that the endogenous CXCR4 antagonist EPI-X4 mobilizes HSCs, a process not only involved in normal hematopoiesis, but also cancer and (I) that peptide fragments of osteopontin (OPN) are associated with the regulation of aging HSCs. The project aims at understanding the role of EPI-X4, OPN and other endogenous peptides in the process of normal and compromised HSC function.. ...

TY - JOUR. T1 - Plasma osteopontin in acute liver failure. AU - The Acute Liver Failure Study Group. AU - Srungaram, Praveen. AU - Rule, Jody A.. AU - Yuan, He Jun. AU - Reimold, Andreas. AU - Dahl, Benny. AU - Sanders, Corron. AU - Lee, William M.. AU - Larson, Anne M.. AU - Liou, Iris. AU - Davern, Timothy. AU - Fix, Oren. AU - Schilsky, Michael. AU - McCashland, Timothy. AU - Hay, J. Eileen. AU - Murray, Natalie. AU - Shaikh, A. Obaid S. AU - Blei, Andres. AU - Ganger, Daniel. AU - Zaman, Atif. AU - Han, Steven H B. AU - Fontana, Robert. AU - McGuire, Brendan. AU - Chung, Raymond T.. AU - Smith, Alastair. AU - Brown, Robert. AU - Crippin, Jeffrey. AU - Harrison, Edwin. AU - Reuben, Adrian. AU - Munoz, Santiago. AU - Reddy, Rajender. AU - Stravitz, R. Todd. AU - Rossaro, Lorenzo. AU - Satyanarayana, Raj. AU - Hassanein, Tarek. PY - 2015/6/1. Y1 - 2015/6/1. N2 - Background: Osteopontin (OPN) is a novel phosphoglycoprotein expressed in Kupffer cells that plays a pivotal role in activating ...

TY - JOUR. T1 - Increased expression of osteopontin is associated with long-term bee venom immunotherapy. AU - Konno, Satoshi. AU - Golden, David B.K.. AU - Schroeder, John. AU - Hamilton, Robert G.. AU - Lichtenstein, Lawrence M.. AU - Huang, Shau Ku. PY - 2005/1/1. Y1 - 2005/1/1. N2 - Background: Venom allergen immunotherapy (VIT) is proven to be highly effective for insect allergy, but the mechanisms and the biomarkers associated with clinical efficacy remain elusive. Objective: The aim of this study was to identify candidate biomarkers associated with successful VIT. Methods: Gene chip array and clustering analyses of PBMCs from subjects with or without VIT were performed. Results: From gene chip array and clustering analyses, an increased expression of osteopontin was found in patients who completed 5 to 6 years of VIT and discontinued therapy for 3 to 6 years (completed treatment group) compared with the untreated group. A significantly higher level of serum osteopontin was found in the ...

Sleeve gastrectoy constitutes an effective procedure for the treatment of morbid obesity. The aim of the present study was to establish in rats the effects of surgically induced weight loss on circulating concentrations in adipose tissue and liver of osteopontin. Effect of Sleeve Gastrectomy on Osteopontin Circulating Levels and Expression in Adipose Tissue and Liver in Rats

Dystrophic calcification (DC) is the calcification occurring in degenerated or necrotic tissue, as in hyalinized scars, degenerated foci in leiomyomas, and caseous nodules. This occurs as a reaction to tissue damage, including as a consequence of medical device implantation. Dystrophic calcification can occur even if the amount of calcium in the blood is not elevated. (A systemic mineral imbalance would elevate calcium levels in the blood and all tissues and cause metastatic calcification.) Basophilic calcium salt deposits aggregate, first in the mitochondria, and progressively throughout the cell. These calcifications are an indication of previous microscopic cell injury. It occurs in areas of cell necrosis in which activated phosphatases bind calcium ions to phospholipids in the membrane. Calcification can occur in dead or degenerated tissue. Caseous necrosis in T.B. is most common site of dystrophic calcification. Liquefactive necrosis in chronic abscesses may get calcified. Fat necrosis ...

Although osteopontin (OPN) is induced in alcoholic patients, its role in the pathophysiology of alcoholic liver disease (ALD) remains unclear. Increased translocation of lipo-polysaccharide (LPS) from the gut is key for the onset of ALD because it promotes macrophage infiltration and activation, tumor necrosis factor-a (TNFa) production, and liver injury. Since OPN is protective for the intestinal mucosa, we postulated that enhancing OPN expression in the liver and consequently in the blood and/or in the gut could protect from early alcohol-induced liver injury. Wild-type (WT), OPN knockout (Opn2/2),and transgenic mice overexpressing OPN in hepatocytes (OpnHEPTg) were fed either the control or the ethanol Lieber-DeCarli diet. Ethanol increased hepatic, plasma, biliary, and fecal OPN more in OpnHEPTg than in WT mice. Steatosis was less in ethanol-treated OpnHEPTg mice as shown by decreased liver-to-body weight ratio, hepatic triglycerides,the steatosis score, oil red-O staining, and lipid peroxidation.

Osteopontin (OPN) is a multifunctional protein that can activate cell-signaling pathways and lead to cancer development and metastasis. Elevated OPN expression was reported in different cancer types, including breast tumors. Here, we present a new immuno-mass spectrometry method for OPN quantification in fresh-frozen malignant and adjacent normal human breast tissues. For quantification we used two proteotypic peptides: OPN-peptide-1 and OPN-peptide-2. Peptide concentrations were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in multiple reaction monitoring (MRM) mode with stable isotope standards (SIS) and immuno-affinity enrichment for isolation of OPN peptides. Based on the OPN-peptide-1, the average OPN concentration in normal breast tissue was 19.42 μg/g, while the corresponding level in breast tumors was 603.9 μg/g. Based on OPN-peptide-2, the average concentration in normal breast tissue was 19.30 μg/g and in breast tumors 535.0 μg/g. In ER/PR/HER2(−) ...

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease caused by environmental, hormonal and genetic factors which lead to immunological abnormalities [1]. Immune system deregulation leads to production of autoantibodies and cytokines. Autoantibodies form complexes with antigens, which are deposited in organs, causing inflammation and tissue damage [2, 3]. The aetiology of SLE is not fully explained but many studies showed the association between the disease and genes crucial to the immunological response [4]. In the literature, there are reports suggesting that osteopontin (OPN) participates in the pathogenesis of SLE. OPN, also known as early T lymphocyte activation-1 (Eta-1) or SPP-1 (secreted phosphoprotein 1), is a protein secreted by various cell types such as bone cells, neurons and immune cells (B and T lymphocytes, natural killer cells, NKT cells, macrophages, neutrophils, dendritic cells) [5, 6]. OPN may be involved in the pathogenesis of SLE as it was found to regulate ...

Osteopontin (OPN) has been implicated as an important mediator of breast cancer progression and metastasis and has been investigated for use as a potential therapeutic target in the treatment of breast cancer. However, the in vivo antitumor effect of anti-OPN antibodies on breast cancer has not been reported. In this study, a mouse anti-human OPN antibody (1A12) was humanized by complementarity-determining region grafting method based on computer-assisted molecular modeling. A humanized version of 1A12, denoted as hu1A12, was shown to possess affinity comparable to that of its parental antibody. The ability of hu1A12 to inhibit cell migration, adhesion, invasion and colony formation was assessed in a highly metastatic human breast cancer cell line MDA-MB-435S. The results indicated that hu1A12 was effective in inhibiting the cell adhesion, migration, invasion and colony formation of MDA-MB-435S cells in vitro. hu1A12 also showed significant efficacy in suppressing primary tumor growth and spontaneous

c-Src is involved in HGF-mediated OPN production in osteoblasts.Cells were pretreated for 30 min with PP2 or transfected with c-Src mutant and siRNA followed by

New research from Sweden indicates that the brains immune system is activated in people with Schizophrenia. This reminded me of Dr. Moreaus discovery that Osteopontin levels in children with Scoliosis are dangerously high. Osteopontin can be a marker for both inflammation and infection. Sciencedaily: (November 18, 2009) Immune System Activated in Schizophrenia In related news NY Times: Study Shows Role of Time and Place of Birth in Schizophrenia

Here we observed that Pn contributes to the cardiac hypertrophic response, an observation that was not anticipated, given its primary function as an ECM cellular adhesion protein. That a cellular adhesion protein might affect the cardiac hypertrophic response is not without precedence, especially given the known importance of integrins in regulating cardiac reactive signaling4,35 and given the known role of Pn as a substrate for multiple combinations of integrins. For example, both focal adhesion kinase and integrin-linked kinase, which are intracellular signaling components of the integrin complex, function as important regulators of the cardiac hypertrophy response.36,37 Indeed, mice lacking the protein osteopontin, which is another cardiac inducible and secreted ECM protein that binds integrins, showed less pressure overload hypertrophy compared with wild-type mice.38 Based on these results, it is tempting to speculate that Pn alters the cardiac hypertrophic response by affecting signaling ...

TY - JOUR. T1 - Osteopontin, E-cadherin, and β-catenin expression as prognostic biomarkers in patients with radically resected gastric cancer. AU - Di Bartolomeo, M.. AU - Pietrantonio, F.. AU - Pellegrinelli, A.. AU - Martinetti, A.. AU - Mariani, L.. AU - Daidone, M.G.. AU - Bajetta, Emillio. AU - Pelosi, G.. AU - de Braud, F.. AU - Floriani, I.. AU - Miceli, R.. N1 - Cited By :3 Export Date: 8 March 2017. PY - 2016. Y1 - 2016. U2 - 10.1007/s10120-015-0495-y. DO - 10.1007/s10120-015-0495-y. M3 - Article. VL - 19. SP - 412. EP - 420. JO - Gastric Cancer. JF - Gastric Cancer. SN - 1436-3291. IS - 2. ER - ...

Immunohistochemical Localization of Osteopontin - A Comparative Study on Periodontally Healthy and Diseased Tooth Root Surface, Julie Toby Thomas, Toby

The authors make gelatin-PEG tyramine gels (GPT gels) that they functionalize with a SVVYGLR peptide. The SVVYGLR sequence, often found near an RGD sequence in different types of ECM, has been shown to improve adhesion, migration, and tube formation of endothelial cells. Crosslinking and bioconjugation of the peptide sequence is done using a horseradish-peroxide (HRP) mediated tyrosine radical dimerization. The authors then demonstrate that human umbilical endothelial cells have better attachment and proliferation on gels containing the SVVYGLR sequence than on gels containing just RGD. They also look at the vascularization of gels subcutaneously injected into rats ...

Upon aging, hematopoietic stem cells (HSCs) undergo changes in function and structure, including skewing to myeloid lineages, lower reconstitution potential and loss of protein polarity. While stem cell intrinsic mechanisms are known to contribute to HSC aging, little is known on whether age‐related changes in the bone marrow niche regulate HSC aging. Upon aging, the expression of osteopontin (OPN) in the murine bone marrow stroma is reduced. Exposure of young HSCs to an OPN knockout niche results in a decrease in engraftment, an increase in long‐term HSC frequency and loss of stem cell polarity. Exposure of aged HSCs to thrombin‐cleaved OPN attenuates aging of old HSCs, resulting in increased engraftment, decreased HSC frequency, increased stem cell polarity and a restored balance of lymphoid and myeloid cells in peripheral blood. Thus, our data suggest a critical role for reduced stroma‐derived OPN for HSC aging and identify thrombin‐cleaved OPN as a novel niche informed therapeutic ...

Background Breasts cancer tumor is 1 of the many diagnosed cancers and accounts for more than 400 frequently, 000 fatalities each full year worldwide. ICAM-1 reflection. The research suggests that inhibition of mTOR by rapamycin augments whereas overexpression of mTOR/g70S6 kinase prevents OPN-induced ICAM-1 reflection. Furthermore, overexpression of mTOR prevents OPN-induced AP-1-DNA and NF-B holding … Continue reading Background Breasts cancer tumor is 1 of the many diagnosed cancers. ...

Most of you already know about ScoliScore, the Axial Biotech test that can help certain populations of kids with scoliosis, determine their risk of curve progression. But, a new test could soon be on the market. The following paper was presented by Alain Moreau (Universite de Montreal), at the SRS meeting that ended earlier today. High Circulating Levels of Osteopontin are Associated with Idiopathic Scoliosis Onset and Spinal Deformity Progression Introduction: We hypothesized that

SCA899Mu, CLIA Kit for Osteopontin (OPN), 骨桥素(OPN)检测试剂盒(化学发光免疫分析法), SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin | 仅供体外研究使用，不用于临床诊断！请索取进口关税税单及报关单！

PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.

Special note about Community Feature Content. Any content and/or opinions uploaded, expressed or submitted through any Community Feature or any other publicly available section of the Web Site (including password-protected areas), and all articles and responses to questions, other than the content explicitly authorized by the Company, are solely the opinions and responsibility of the person or entity submitting them and do not necessarily reflect the opinions of the Company. By way of example, any recommended or suggested use of products or services available from the Company that is posted through a Community Feature is not a sign of approval or recommendation by the Company. If you choose to follow any such recommendation you do so at your own risk.. Links to Third Party Sites. The Web Site may contain links to other websites on the internet. The Company is not responsible for the content, products, services or practices of any third party websites, including without limitation sites linked to ...

Pathological calcification Definition: it is the deposition of calcium salts in sites other than bones and teeth. Grossly: the calcified tissue appears chalky white and hard. Microscopically, the calcification appears as blue granules (with haematoxylin and eosin stain). There are two types of pathological calcification: 1. Dystrophic calcification: it is the most common type. Definition: […]. Tags: calcification, Pathological, type, unknown. ...

Uranium, Affect, Cell, DNA, Human, Ions, Kidney, Osteopontin, Proteins, Set, Time, Calcium, A Gene, Bone, Cell Lines, Cells, Coding, DNA Microarrays, Gene, Gene Expression

OPN1LW, crveno-senzitivni opsin, je protein koji je kod ljudi kodiran OPN1LW genom.[1] Ovaj gen kodira pigment iz familije opsina koji apsorbuje svetlost. On se naziva crveni fotopigment kupastih ćelija, ili opsin senzitivan na dugačke talasne dužine. Opsini su grupa G-protein spregnutih receptora sa sedam transmembranskih domena, ekstracelularnim N-terminusnim domenom, i citosolnim C-terminusom. Ovaj gen i opsinski geni srednjih-talasnih dužina su grupisani na X hromozomu. Česte neravnomerne rekombinacije i konverzije gena se mogu javiti između tih sekvenci. X hromozomi mogu da imaju spajanja gena opsina srednje i duge talasne dužine, ili višestruke kopije tih gena. Defekti ovog gena su uzrok parcijalne slepoće za boje.[1] ...

Roylan Foam Crt Opn Cell 11x24 Med Ca - Model CA4882 : This product is a non stock item that must be ordered from the manufacturer. As such, will app