To study the correlations between serum osteocalcin and glucose metabolism in patients with type 2 diabetes, 66 cases were collected to determine total osteocalcin, undercarboxylated osteocalcin, fasting blood glucose, fasting insulin, and HbA1c. Osteocalcin concentrations were compared between groups of different levels of HbA1c, and parameters of glucose metabolism were compared between groups of different levels of total osteocalcin and undercarboxylated osteocalcin. The relationship between osteocalcin and parameters of glucose metabolism was also analyzed. We found that the total osteocalcin concentration of high-HbA1c group was significantly lower than that of low-HbA1c group. The fasting blood glucose of low-total-osteocalcin group was significantly higher than that of high-total-osteocalcin group in male participants, while the fasting blood glucose of low-undercarboxylated-osteocalcin group was significantly higher than that of high-undercarboxylated-osteocalcin group in all participants and in

Monoclonal Anti-Bovine Osteocalcin Antibody (Clones OC4-30,OCG2, OCG3 and OCG4) is raised against bovine osteocalcin and recognizes both bovine and human osteocalcins. Clone OC4-30 is specific for position 17 gamma-carboxylated osteocalcin and does not recognize decarboxylated osteocalcin. Osteocalcin epitopes used to generate Clones OCG2, OCG3 and OCG4 are amino acids 45-49, amino acids 21-31 and amino acids 4-9, respectively. This collection of osteocalcin antibody clones facilitates osteocalcin immunohistochemistry studies which can also be performed on paraffin-embedded tissues (as described in the Resources). In addition to cross-reactivity with bovine and human osteocalcin, clones OCG4-30, OCG3 and OCG4 are also capable of recognizing osteocalcin in several other species as listed. For species-specific osteocalcin detection, Takara Bio offers additional osteocalcin antibody products. ...

Monoclonal Anti-Bovine Osteocalcin Antibody (Clones OC4-30,OCG2, OCG3 and OCG4) is raised against bovine osteocalcin and recognizes both bovine and human osteocalcins. Clone OC4-30 is specific for position 17 gamma-carboxylated osteocalcin and does not recognize decarboxylated osteocalcin. Osteocalcin epitopes used to generate Clones OCG2, OCG3 and OCG4 are amino acids 45-49, amino acids 21-31 and amino acids 4-9, respectively. This collection of osteocalcin antibody clones facilitates osteocalcin immunohistochemistry studies which can also be performed on paraffin-embedded tissues (as described in the Resources). In addition to cross-reactivity with bovine and human osteocalcin, clones OCG4-30, OCG3 and OCG4 are also capable of recognizing osteocalcin in several other species as listed. For species-specific osteocalcin detection, Takara Bio offers additional osteocalcin antibody products. ...

The skeletal system is considered an endocrine organ today. Associations between bone Gla proteins, body mass index and insulin resistance are intriguing novel field due to being possible explanations for the interactions between bone and endocrine system. The aim of the present study was to investigate the associations between insulin resistance and body mass index (BMI) with plasma osteocalcin, phylloquinone levels and dietary vitamin K intakes in healthy non-obese adults. This cross-sectional study was conducted with 77 healthy non-obese adults. Anthropometric measurements and 24-hour food consumption record were taken from each individual. Blood glucose, insulin, osteocalcin (OC), undercarboxylated osteocalcin (ucOC), vitamin K levels were analyzed. The homeostasis model assessment for insulin resistance (HOMA-IR) value was calculated. Multiple linear regression models were performed using the backward method in order to determine the significant predictors of BMI and HOMA-IR. Mean age and ...

Evidences indicate that skeleton works not only as a structural scaffold but also as an endocrine organ. Many bioactive factors secreted from bone, such as osteocalcin and osteoprotegerin, have a regulatory role in energy metabolism [21, 22]. Recent studies have suggested a potentially important role for osteocalcin in regulating the function of vascular endothelial cells, and the cardiovascular system [23, 24]. This suggests osteocalcin could be involved in the development of CAD. Several studies have investigated the association between osteocalcin and atherosclerosis, but results have not been consistent. Some studies suggest osteocalcin may actually be protective against early atherosclerosis. For example, a study from Korea demonstrated an independent effect of osteocalcin on vascular endothelial cells, suggesting that osteocalcin could have beneficial effects on atherosclerosis [25]. Our previous study in high fat diet animals indicate that osteocalcin has an endothelial-protective effect ...

Abstract Aim: Serum osteocalcin was shown in a previous study on first trimester pregnant women to correlate with bone density and to distinguish between fast and slow bone losers. The objective of the present study is to examine whether serum osteocalcin is related to vitamin D receptor (VDR) BsmI polymorphism in pregnant women. Study design: We determined osteocalcin serum levels and VDR BsmI genotype in 97 healthy first trimester pregnant women consecutively recruited during six months. Results: BB (21%), Bb (38%) and bb (41%) genotypes showed similar osteocalcin serum levels. However, in primigravidas (ns38) the BB genotype was significantly associated with higher mean osteocalcin level (9.67 ng/mL) than the Bb (8.07 ng/mL) and the bb genotype (8.14 ng/mL), respectively (P-0.05). The VDR genotype was the only independent parameter to correlate with serum osteocalcin (P-0.05). Conclusion: Only primigravidas show in the first trimester a relation between the bone formation parameter serum ...

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In a high-profile, 2019 study published in Cell Metabolism, researchers measured significant increases in serum Osteocalcin levels in both humans and mice, in response to an acute stressor, with evidence to indicate Osteocalcins direct contribution to the bodys fight or flight response. This study demonstrated that the increased release of osteocalcin from bones in response to acute stress lead to receptor mediated downstream signaling of post-ganglionic parasympathetic neurons to inhibit their cholinergic activity (1). The study identified the bone as a stress organ and osteocalcin as a stress hormone capable of evolutionarily conferring a survival advantage to bony vertebrates. Increased Osteocalcin levels have been observed within minutes of the application of a stressor in both mice and humans in several studies prior to this one, and its role in stress is well documented (15-16).. In conjunction with exercise, osteocalcin may be a vital catalyst for the increase of interleukin-6 which is ...

Circulatory osteocalcin (OC) has been widely used as a biomarker to indicate bone turnover status in postmenopausal osteoporosis (PMO). However, the change of serum OC (sOC) level in PMO cases compared to postmenopausal controls remains controversial. We searched the online database of PubMed and Cochrane Library. A meta-analysis of case-control studies was performed to compare the pooled sOC level between PMO patients and postmenopausal controls. Subgroup analysis according to potential confounding factors (different OC molecules and regions of the study population) was also performed. Ten case-control studies with 1577 postmenopausal women were included in this meta analysis. We found no significant difference in the pooled sOC level [mean difference (MD) = 1.84, 95% confidence interval (CI): (− 1.49, 5.16), p = 0.28] between PMO patients and controls. Subgroup analysis also revealed no significant difference in intact OC [MD = 1.76, 95%CI: (− 1.71, 5.23), p = 0.32] or N-terminal mid-fragment of

Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein hormone found in bone and dentin. Because it has gla domains, its synthesis is vitamin K dependent. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A. Osteocalcin is secreted solely by osteoblasts and thought to play a role in the bodys metabolic regulation and is pro-osteoblastic, or bone-building, by nature. It is also implicated in bone mineralization and calcium ion homeostasis. Osteocalcin acts as a hormone in the body, causing beta cells in the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Osteocalcin acts on Leydig cells of the testis to stimulate testosterone biosynthesis and therefore affect male fertility. Osteocalcin also acts on myocytes to promote energy availability and utilization and in this manner favors exercise capacity. As ...

In this study we used a mouse model system to compare the in vivo effects of parathyroid hormone(1-34) [PTH(1-34)] with that of PTH(1-31) or PTH(2-34) analogs. Daily subcutaneous administration of PTH(1-34) for 15 days caused a dose-dependent increase in the serum osteocalcin level and bone extract alkaline phosphatase activity, markers of bone formation. PTH(2-34) was much less potent, whereas PTH(1-31) was equipotent in stimulating bone formation parameters in mice. PTH(1-34) caused significant increases in serum calcium (after 4 h) and tartrate-resistant acid phosphatase activity in bone extract (after 4 h), whereas PTH(2-34) and PTH(1-31) were less potent. Because PTH(1-31) caused a smaller increase in bone resorption parameters compared to PTH(1-34), despite similar effects on bone formation parameters, we evaluated the long-term anabolic effects of PTH(1-31) and PTH(1-34) in mice. Weekly evaluations of serum osteocalcin levels demonstrated that daily injections of PTH(1-34) and PTH(1-31) ...

The purpose of this study was to examine if the reduction in glucose post-exercise is mediated by undercarboxylated osteocalcin (unOC). Obese men were randomly assigned to do aerobic or power exercise

This study aimed to investigate the relationships among osteocalcin, leptin and metabolic health outcomes in children ages 9-13 years. This was a cross-sectional analysis of baseline data from 161 boys and 157 girls (ages 9-13 years) who previously participated in a double-blinded randomized placebo controlled trial of vitamin D supplementation. Relationships among fasting serum total osteocalcin (tOC), undercarboxylated osteocalcin (ucOC), leptin, and metabolic health outcomes were analyzed. Approximately 52% of study participants were obese based on percent body fat cutoffs (|25% for boys and |32% for girls) and about 5% had fasting serum glucose within the prediabetic range (i.e. 100 to 125 mg/dL). Serum tOC was not correlated with leptin, glucose, insulin, HOMA-IR, or HOMA-β after adjusting for percent body fat. However, serum ucOC negatively correlated with leptin (partial r = −0.16; p = 0.04) and glucose (partial r = −0.16; p = 0.04) after adjustment for percent body fat. Leptin was a

TY - JOUR. T1 - The dichotomy in the effects of 1,25 dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3 on bone γ-carboxyglutamic acid-containing protein in serum and bone in vitamin D-deficient rats. AU - Wientroub, Shlomo. AU - Price, Paul A.. AU - Reddi, A Hari. PY - 1987/5. Y1 - 1987/5. N2 - Vitamin D-deficient, second generation, rachitic rats showed significant decrease in bone Gla protein (BGP) levels in circulation and in the skeleton. 1,25 dehydroxyvitamin D3 (1,25 (OH)2D3) exhibited the most potent influence on serum BGP levels in a dose-dependent manner. At a dose 25 ng/100 g body weight 1,25 (OH)2D3 showed a cumulative effect, i.e., the longer the treatment, the more circulating BGP was detected 24,25 dehydroxyvitamin D3 (24,25(OH)2D3) at the same doses did not show similar effect on the serum BGP levels, regardless of the serum calcium levels. Bone BGP levels assayed at various sites representing endochondral and intramenbranous ossification demonstrated an opposite pattern. ...

In order to compare the induced time of osteoporosis between ovariectomized and neurectomized models in ddY mice. Experimental groups were divided into Sham, ovariectomized (OVX group) and neurectomized (NX group) group. The changes of body weight, tibia weight and histomorphometry of epiphyseal regions of tibia that were generally used as criteria index in osteoporosis, were evaluated at 2 and 4 weeks after operations with other generally used index-changes of serum osteocalcin. Also, calcium and phosphorus levels in the ash tibia were demonstrated with their ratio (Ca/P ratio). From the result of this study, evidences which reflect osteoporotic states of animals such as decrease of absolute and relative tibia weight, histomorphometrical index of epiphyseal region of tibia including trabecular bone volume %, and calcium and phosphorous contents in tibia, were generally detected from 4 weeks after ovariectomy and 2 weeks after neurectomy with increase of serum osteocalcin levels. In conclusion, it

and Thomasset M. (1990) Endocrinology 127:580-587). The bone-oriented assays include: 1) assessment of bone resorption as determined via the release of Ca2+ from bone in vivo (in animals fed a zero Ca2+diet) (Hibberd K.A. and Norman A.W. (1969) Biochem. Pharmacol 18:2347-2355; Hurwitz S. etal (1967)7. Nutr. 91:319-323), or from bone explants in vitro (Bouillon R. et al (1992) J. Biol Chem. 267:3044-3051), 2) measurement of serum osteocalcin levels [osteocalcin is an osteoblast-specific protein that after its synthesis is largely incorporated into the bone matrix, but partially released into the circulation (or tissue culture medium) and thus represents a good market of bone formation or turnover] (Bouillon R. et al (1992) Clin. Chem. 38:2055-2060), or 3) bone ash content (Norman A.W. and Wong R.G. (1972) J. Nutr. 102:1709-1718). Only one kidney-oriented assay has been employed. In this assay, urinary Ca2+ excretion is determined (Hartenbower D.L. et al (1977) Walter de Gruyter, Berlin pp ...

The accessibility of regulatory elements in chromatin represents a principal rate-limiting parameter of gene transcription and is modulated by enzymatic transcriptional co-factors that alter the topology of chromatin or covalently modify histones (e.g. by acetylation). The bone-specific activation and 1,25-dihydroxyvitamin D(3) enhancement of osteocalcin (OC) gene transcription are both functionally linked to modifications in nucleosomal organization. The initiation of tissue-specific basal transcription is accompanied by the induction of two DNase I hypersensitive sites, and this chromatin remodeling event requires binding of the key osteogenic factor RUNX2/CBFA1 to the OC promoter. Here, we analyzed the acetylation status of histones H3 and H4 when the OC gene is active (in osteoblastic ROS17/2.8 cells) or inactive (in fibroblastic ROS24/1 cells) using chromatin immunoprecipitation assays. We find that acetylated histone H3 and H4 proteins are associated with the OC promoter only when the gene is

Background Many studies have identified smoking like a risk factor for osteoporosis, but it is usually unclear whether passive smoking has an effect on bone mineral density and bone turnover and if such an effect could cause osteoporosis. rats and in control rats. Outcomes 57420-46-9 manufacture BMD of lumbar femur and backbone was low in 4-month smoke-exposed feminine rats than that in handles. However, there is no factor in serum osteocalcin levels between smoke-exposed controls and rats. Considerably more affordable b-ALP and larger TRACP 5b were within the 4-month or 3-month smoke-exposed rats in comparison to controls. Subsequent analysis demonstrated that b-ALP favorably correlated with BMD from the lumbar vertebrae(r = 0.764, P = 0.027) and femur(r = 0.899, P = 0.002) in 4-month smoke-exposed feminine rats. Furthermore, TRACP 5b amounts adversely correlated with BMD of lumbar vertebrae (r = -0.871, P = 0.005) and femur (r = -0.715, P = 0.046) in 4-month smoke-exposed feminine rats. Bottom ...

Application value of serum osteocalcin and bone mineral density detection in middle-aged and elderly healthy physical examination - Minerva Medica 2020 Jun 26 - Minerva Medica - Journals

Low total osteocalcin levels are associated with insulin resistance, diabetes, and metabolic syndrome. Researchers are exploring this protein in their search for new therapies for type 2 diabetes.

Immunostaining for osteocalcin in the aortic valve in Older mice.In vehicle-treated mice (A,C), osteocalcin (dark brown) is abundant near the cusp base (arrows)

Results Sixteen patients (50% women) with a mean age of 56.1±12.8 years, disease duration 18.7±11.4 years, median BASDAI score 4.2 range (1.3-8.1) and BASMI 4.4 (1.0-6.6) were included. Five patients dropped out during the 2 years. No severe adverse events occurred. BMD increased by 9.9±5.7% (p=0.003) in lumbar spine, 3.0±3.3% (p=0.016) in total hip and by 4.2±13.5% (NS) in distal radius. During the 2-year trial serum levels of Wnt3a decreased from median 3.88 range (2.93-6.21) ng/ml to 1.74 (1.08-2.84) ng/ml (p,0.001), OPG decreased from 4.07 (2.08-7.78) pmol/l to 3.06 (1.35-4.54) (p,0.001), CTX-I decreased from 0.50 (0.16-1.34) ng/ml to 0.18 (0.07-0.41) ng/ml (p,0.001) and osteocalcin decreased from 20.11 (11.55-59.78) ng/ml to 7.94 (5.63-14.13) ng/ml (p,0.001). The other biomarkers did not change significantly during the 2-year follow-up.. ...

Secondary outcome measure- Bone Turnover as assessed by the biochemical markers (serum CTX, P1NP, BALP, carboxylated and undercarboxylated osteocalcin (OC), OPG). These markers will be measured at the same time point during each clinic visit ...

Free Online Library: The Role of Osteocalcin and Alkaline Phosphatase Immunohistochemistry in Osteosarcoma Diagnosis.(Research Article) by Pathology Research International; Health, general EDTA Ethylenediaminetetraacetic acid Phosphatases

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PMF1-BGLAP, 0.5 mg. This locus represents naturally occurring read-through transcription between the neighboring PMF1 (polyamine-modulated factor 1) and BGLAP (bone gamma-carboxyglutamate Gla protein) genes on chromosome 1.

We have brought together our full range of anti-osteocalcin antibodies in one place, making it easier for you to find exactly what you need.

Этот документ предоставляет пример конфигурации с помощью IPv6, который помогает вам настраивать BGP Prefix-Based Outbound Route Filtering. Эта функция использует фильтр маршрута исходящего потока (ORF) BGP, передают и получают возможности, которые минимизируют количество Обновлений BGP, передаваемых между равными маршрутизаторами. Настройка эта функция может помочь в отфильтровывании нежелательных обновлений маршрута в источнике.

TY - JOUR. T1 - Bone-specific transcription factor Runx2 interacts with the 1α,25-dihydroxyvitamin D3 receptor to up-regulate rat osteocalcin gene expression in osteoblastic cells. AU - Paredes, Roberto. AU - Arriagada, Gloria. AU - Cruzat, Fernando. AU - Villagra, Alejandro. AU - Olate, Juan. AU - Zaidi, Kaleem. AU - Van Wijnen, Andre. AU - Lian, Jane B.. AU - Stein, Gary S.. AU - Stein, Janet L.. AU - Montecino, Martin. PY - 2004/10. Y1 - 2004/10. N2 - Bone-specific transcription of the osteocalcin (OC) gene is regulated principally by the Runx2 transcription factor and is further stimulated in response to 1α,25-dihydroxyvitamin D3 via its specific receptor (VDR). The rat OC gene promoter contains three recognition sites for Runx2 (sites A, B, and C). Mutation of sites A and B, which flank the 1α,25-dihydroxyvitamin D3-responsive element (VDRE), abolishes 1α,25-dihydroxyvitamin D3-dependent enhancement of OC transcription, indicating a tight functional relationship between the VDR and ...

Genetic studies show that Msx2 and Dlx5 homeodomain (HD) proteins support skeletal development, but null mutation of the closely related Dlx3 gene results in early embryonic lethality. Here we find that expression of Dlx3 in the mouse embryo is associated with new bone formation and regulation of osteoblast differentiation. Dlx3 is expressed in osteoblasts, and overexpression of Dlx3 in osteoprogenitor cells promotes, while specific knock-down of Dlx3 by RNA interference inhibits, induction of osteogenic markers. We characterized gene regulation by Dlx3 in relation to that of Msx2 and Dlx5 during osteoblast differentiation. Chromatin immunoprecipitation assays revealed a molecular switch in HD protein association with the bone-specific osteocalcin (OC) gene. The transcriptionally repressed OC gene was occupied by Msx2 in proliferating osteoblasts, while Dlx3, Dlx5, and Runx2 were recruited postproliferatively to initiate transcription. Dlx5 occupancy increased over Dlx3 in mature osteoblasts at the

PURPOSE: Cranberry juice (CJ) contains a remarkably high concentration of polyphenols, considered to be beneficial for cardiovascular and bone health. The current double-blind, randomized study was designed to test whether daily consumption of double-strength Ocean Spray light CJ (2 x 230 ml) over 4 months has beneficial effects on vascular function and on endothelial progenitor cells (EPCs) carrying the osteoblastic marker osteocalcin in particular. METHODS: A total of 84 participants (49.5 +/- 16.2 years) with peripheral endothelial dysfunction and cardiovascular risk factors were enrolled in this double-blind, randomized, controlled trial (69 completed the 4-month protocol-32 in the CJ group and 37 in the placebo group, respectively). Vascular responses to reactive hyperemia were measured non-invasively by peripheral arterial tonometry (EndoPAT). Peripheral blood mononuclear cells were stained for EPC markers, as well as osteocalcin, and counted by flow cytometry. RESULTS: Baseline ...

TY - JOUR. T1 - Localization of the osteocalcin gene cluster on mouse Chromosome 3. AU - Desbois, C.. AU - Seldin, Michael F. AU - Karsenty, G.. PY - 1994/5. Y1 - 1994/5. UR - http://www.scopus.com/inward/record.url?scp=0028427906&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0028427906&partnerID=8YFLogxK. U2 - 10.1007/BF00389550. DO - 10.1007/BF00389550. M3 - Article. C2 - 7915557. AN - SCOPUS:0028427906. VL - 5. SP - 321. EP - 322. JO - Mammalian Genome. JF - Mammalian Genome. SN - 0938-8990. IS - 5. ER - ...

PAA471Ra01, Polyclonal Antibody to Osteocalcin (OC), 骨钙素(OC)多克隆抗体, BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein | 仅供体外研究使用，不用于临床诊断！请索取进口关税税单及报关单！

The aims of the present study are to investigate the effect of vitamin K2 on bone turnover, bone mass, bone structure, glucose metabolism, and arteriosclerosis.. Osteoporosis, diabetes, metabolic syndrome and cardiovascular disease are common diseases that affect large groups of people in the Western world.. Our hypotheses is that vitamin K2 (MK-7) reduces undercarboxylated osteocalcin in postmenopausal women and reduces bone turnover and increases bone mineral density; increases insulin sensitivity and decreases indices of arterial calcification. ...

XRCC5_HUMAN] Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3-5 direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5-deoxyribose-5-phosphate lyase (5-dRP lyase), by catalyzing the ...

Osteocytes have been proposed to be the cells primarily responsible for sensing the effects of mechanical loading in bone. Osteocytes respond to loading in vivo, and have been shown to express osteotropic agents and their receptors, and cell/matrix adhesion molecules in vitro, but the functional significance of such findings is not clear. One obstacle to increased understanding of the role of osteocytes in the regulation of bone mass is that the cells are not easily accessible for study. In situ studies are difficult, and although it is possible to extract and culture osteocytes from neonatal bones, the responses of such cells might be very different from those in older bones in situ. We have developed a technique to investigate osteocyte gene expression in vivo, using the reverse transcriptase linked polymerase chain reaction (PCR), and have shown that they express mRNA for β-actin (β-ACT), osteocalcin (OC), connexin-43 (Cx43), insulin-like growth factor I (IGF-I), c-fos, and c-jun, but not ...

Currently, no model is available to study the cellular and molecular events associated with bone metastases of prostate cancer. This study shows that MDA PCa 2a and MDA PCa 2b cells induce a specific and reproducible increase in osteoblast differentiation and proliferation when the cells share the medium during coculturing. Osteoblast differentiation in this system was associated with up-regulation of the osteoblast-specific transcriptor factor Cbfa1. Moreover, up-regulation of Cbfa1 and Osteocalcin expression was also induced in PMOs by CM produced by MDA PCa 2b cells, suggesting that soluble factors produced by prostate cancer cells promote osteoblast differentiation and that Cbfa1 mediates this effect. To our knowledge, this is the first in vitro model of bone metastasis from prostate cancer that recapitulates the osteoblastic phenotype typical of the disease. These results confirmed in vivo and at the molecular level, suggest that the pathophysiology of osteoblastic bone metastases from ...

Medical definition of osteocalcin: a protein that is found in the extracellular matrix of bone and in the serum of circulating blood, is produced by …

A specific oligodeoxynucleotide (ODN), ODN MT01, was found to have positive effects on the proliferation and activation of the osteoblast-like cell line MG 63. In this study, the detailed signaling pathways in which ODN MT01 promoted the differentiation of osteoblasts were systematically examined. ODN MT01 enhanced the expression of osteogenic marker genes, such as osteocalcin and type I collagen. Furthermore, ODN MT01 activated Runx2 phosphorylation via ERK1/2 mitogen-activated protein kinase (MAPK) and p38 MAPK. Consistently, ODN MT01 induced up-regulation of osteocalcin, alkaline phosphatase (ALP) and type I collagen, which was inhibited by pre-treatment with the ERK1/2 inhibitor U0126 and the p38 inhibitor SB203580. These results suggest that the ERK1/2 and p38 MAPK pathways, as well as Runx2 activation, are involved in ODN MT01-induced up-regulation of osteocalcin, type I collagen and the activity of ALP in MG 63 cells.

The aim of the GH-2000 project is to develop a method for detecting GH doping among athletes. Previous papers in the GH-2000 project have proposed that a forthcoming method to detect GH doping will need specific components from the GH/IGF-I axis and bone markers because these specific variables seem more sensitive to exogenous GH than to exercise. The present study examined the responses of the serum concentrations of these specific variables to a maximum exercise test in elite athletes from selected sports. A total of 117 elite athletes (84 males and 33 females; mean age, 25 yr; range, 18-53 yr) from Denmark, the United Kingdom, Italy, and Sweden participated in the study. The serum concentrations of total GH, GH22 kDa, IGF-I, IGF binding protein (IGFBP)-2, IGFBP-3, acid-labile subunit, procollagen type III (P-III-P), and the bone markers osteocalcin, carboxy-terminal cross-linked telopeptide of type I collagen (ICTP), and carboxy-terminal propeptide of type I procollagen were measured. The ...

BSAP can be mildly elevated in patients with fractures. In addition, patients with hyperparathyroidism, Paget disease, or osteomalacia can have elevations of BSAP. Serum OC levels, if high, indicate a... more

Evidence-based information including studies, news articles etc. about the role of nutrition, diets, foods, nutrients and supplements, in the prevention and complementary treatment of diseases.

Bone-related diseases and dysfunctions are heavy burdens on our increasingly aged society. One important strategy to relieve this problem is through early detection and treatment of bone-related diseases. Towards this goal, there has been constant interest in developing novel bone-specific materials for imag

Order monoclonal and polyclonal Osteocalcin antibodies for many applications. Selected quality suppliers for anti-Osteocalcin antibodies.

TOV Kompjuternie Merezhi (AS35213) is a 16 year old BGP network that is peering with 122 other networks and has 4 upstream carriers

Today while trying to do some lab on BGP, I came across a situation where in I was not able to create BGP peer. I was getting % Create the peer-group first although this was not necessary at all I guess. I have shown the exact result here: R3#configure terminal Enter configuration commands, one per…

Undercarboxylated osteocalcin (ucOC) is reported to function as an endocrine hormone, affecting glucose metabolism in mice (1,2). Vitamin K, which converts ucOC to carboxylated osteocalcin (cOC), has been suggested to regulate glucose metabolism by modulating osteocalcin and/or proinflammatory pathway (3-5).. We studied whether modulation of ucOC via vitamin K2 supplementation for 4 weeks affects β-cell function and/or insulin sensitivity in healthy young male subjects. Forty-two healthy young male volunteers received vitamin K2 (menatetrenone; 30 mg; Eisai Co., Japan) or placebo t.i.d. for 4 weeks. Frequently sampled intravenous glucose tolerance test was performed to determine insulin sensitivity index (Si), acute insulin response to glucose (AIRg), and disposition index (DI) before and after treatment. Adiponectin, interleukin (IL)-6, C-reactive protein (CRP), ucOC, and cOC were measured before and after treatment.. After excluding frequently sampled intravenous glucose tolerance test ...

Results By comparing gene expression profiles in RNA from mice treated with corticosterone or placebo, we found that corticosterone specifically regulated 391 genes. To further investigate the genes targeted by corticosterone treatment we performed gene ontology analysis with the aid of heat maps. We found that the expression of genes implicated in osteoblast differentiation and the regulation of bone remodelling was downregulated in mice treated with corticosterone compared to placebo. We observed a downregulation of the osteoblast markers Runx2, Colα1, osteocalcin and sclerostin in corticosterone-treated mice compared to placebo. In addition BMP4 and BMP7 followed the same pattern. Genes that were most profoundly downregulated in the array analysis were validated by qRT-PCR.. Consistent with mRNA levels, osteocalcin serum levels were suppressed to almost undetectable levels.. ...

ビタミンK依存性Gla化蛋白オステオカルシンはテリパラチドの骨治癒促進効果に影響する. The effect of Vitamin K-dependent Gla protein osteocalcin to accelerate bone repair ...

We know muscle function & decline can lead to increased mortality [its called sarcopenia] particularly in HIV & several studies report leg muscle strength improves brain & cognition function in a study of older long duration athletes ...

Assayed, liquid human serum based control for Intact PTH, Anti-Tg, Anti-TPO, 25-OH Vitamin D, IGF-I, C-Peptide, Osteocalcin, and EPO; level 2 of 4 (6 x 5 mL)

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Males. ,2 years: 25-221 mcg/L. 2-9 years: 27-148 mcg/L. 10-13 years: 35-169 mcg/L. 14-17 years: 13-111 mcg/L. Adults: ≤20 mcg/L. Females. ,2 years: 28-187 mcg/L. 2-9 years: 31-152 mcg/L. 10-13 years: 29-177 mcg/L. 14-17 years: 7-41 mcg/L. Adults. Premenopausal: ≤14 mcg/L. Postmenopausal: ≤22 mcg/L. ...

Fr. Chris Metropulos, Executive Director of the Orthodox Christian Network (OCN), is reminding Orthodox Christians that November is themed Morality and Popular Culture month on OCN.

Brand: Fesser Source: Performance-PCs Purchase Date: 9/24/2011 Color:White Transparent? (y/n): N Liquid / Additives: Distilled water Kill Coil (y/n): N PH Balance:...