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Looking for Organic components? Find out information about Organic components. 1. of, relating to, derived from, or characteristic of living plants and animals 2. of or relating to animal or plant constituents or products having a... Explanation of Organic components
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Staining agarose gels with SYBR Green I does not interfere with the transfer of nucleic acids to membranes or subsequent hybridization in Southern or Northern blot analysis as long as 0.1% -0.3% SDS is included in prehybridization and hybridization buffers to remove the dye ...
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The majority of streams and rivers are ecologically impaired or threatened with high losses in biodiversity, which compromise the future provisioning of vital ecosystem services (1, 2). Understanding the causes of these impairments is crucial to inform freshwater management and for directing restoration efforts (3). Despite their ubiquitous global use, organic chemicals have only been shown to affect aquatic communities locally or regionally, whereas the overall extent of their impact is largely unknown (4, 5). Previous studies on the risk assessment of organic chemicals have been limited to a few sites (6), regions (7), or compounds (8) rendering the extrapolation to larger spatial scales questionable. To date, large-scale analyses have been hindered by the lack of large-scale monitoring databases for organic chemicals and by the scarcity of empirical toxicity data (9). Gaps in missing experimental toxicity data can be filled by modeled or predicted toxicity data from read-across methods (10) ...
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Fluorescence differential display. FDD was performed according to Ito et al. (1994). In brief, 2.5 μg of DNA-free total RNAs, prepared from cerebella of ICR mice (Nippon SLC, Shizuoka, Japan) at various developmental stages [embryonic day 18 (E18), P0, P3, P7, P12, P15, P21, and 8-week-old], were reverse-transcribed using a Superscript II reverse transcriptase (Life Technologies, Rockville, MD) in the presence of 2.5 μm anchored oligo-dT primers 5′-GT15A-3′. PCR was performed using an ExTaq polymerase kit (Takara Shuzo, Kyoto, Japan), the anchored primer, and arbitrary primers (10-mer), essentially as described by Ito et al. (1994). Clone 13-2 was generated using 5′-GTTTTCGCAG-3′ as an arbitrary primer. The PCR products were separated on a 6% polyacrylamide gel and analyzed by a computerized-fluorescent image analyzer FluorImager (Amersham Pharmacia Biotech, Piscataway, NJ) after staining with the fluorescent dye SYBR Green I (Takara Shuzo). Bands of interest were excised from FDD gels ...
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401 KAR 61:175. Leaks from existing synthetic organic chemical and polymer manufacturing equipment.. RELATES TO: KRS 224.20-100, 224.20-110, 224.10-120, 40 C.F.R. 60 Appendix A (Method 21), 42 U.S.C. 7410. STATUTORY AUTHORITY: KRS 224.10-100. NECESSITY, FUNCTION, AND CONFORMITY: KRS 224.10-100 requires the Environmental and Public Protection Cabinet to prescribe administrative regulations for the prevention, abatement and control of air pollution. 42 USC 7410 likewise requires the state to implement standards for national primary and secondary ambient air quality. This administrative regulation provides for the control of volatile organic compound emissions from leaks from existing synthetic organic chemical and polymer manufacturing equipment.. Section 1. Definitions. As used in this administrative regulation, all terms not defined in this section shall have the meaning given to them in 401 KAR 61:001.. (1) Affected facility means each individual component which contacts a process fluid that ...
401 KAR 61:175. Leaks from existing synthetic organic chemical and polymer manufacturing equipment.. RELATES TO: KRS 224.20-100, 224.20-110, 224.10-120, 40 C.F.R. 60 Appendix A (Method 21), 42 U.S.C. 7410. STATUTORY AUTHORITY: KRS 224.10-100. NECESSITY, FUNCTION, AND CONFORMITY: KRS 224.10-100 requires the Environmental and Public Protection Cabinet to prescribe administrative regulations for the prevention, abatement and control of air pollution. 42 USC 7410 likewise requires the state to implement standards for national primary and secondary ambient air quality. This administrative regulation provides for the control of volatile organic compound emissions from leaks from existing synthetic organic chemical and polymer manufacturing equipment.. Section 1. Definitions. As used in this administrative regulation, all terms not defined in this section shall have the meaning given to them in 401 KAR 61:001.. (1) Affected facility means each individual component which contacts a process fluid that ...
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The annual Synthetic Organic Chemicals (SOC) reports provided information on U.S. production and sales of synthetic organic chemicals and the raw materials from which they are made. Chapter 1 of each report presents more information on the scope of the reports; the authority under which the data were collected; and the method used to collect the data.
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Netherlands exports of organic chemicals was US$16.87 Billion during 2019, according to the United Nations COMTRADE database on international trade. Netherlands exports of organic chemicals - data, historical chart and statistics - was last updated on October of 2020.
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Deoxy+ Real-time SYBR Green RT-PCR system provides user with a rapid and simple way to quantify the expression of gene of interest based on Real-time PCR system containing SYBR Green. Yeasterns Deoxy+ HiSpec RT, Hotstart DNA polymerase and all the components for Real-time SYBR Green RT-PCR are skillfully mixed within a single tube. Unique buffer system allows highly specific quantification by preventing the formation of nonspecific products and primer-dimers.. ...
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Our lncRNA gene expression products include sample to insight solutions to enable quick, reliable gene expression analysis. As leaders in the field of high-performance SYBR® Green real-time PCR analysis, our RT2 lncRNA PCR Arrays with comprehensive, easy-to-use data analysis tools deliver focused and accurate results, allowing you to overcome the bottlenecks in your gene expression studies. We provide you with the products that allow you to spend less time at the bench and more time interpreting your results ...
Our lncRNA gene expression products include sample to insight solutions to enable quick, reliable gene expression analysis. As leaders in the field of high-performance SYBR® Green real-time PCR analysis, our RT2 lncRNA PCR Arrays with comprehensive, easy-to-use data analysis tools deliver focused and accurate results, allowing you to overcome the bottlenecks in your gene expression studies. We provide you with the products that allow you to spend less time at the bench and more time interpreting your results ...
Over-expression SDF-1 in AdV.SDF-1 group increased the expression of VEGF and bFGF mRNA analyzed in the myocardium infarction area (Fig. 4) after MI, and VEGF and bFGF expression increased obviously in 3 day after MI and continued until 7 28 days after MI.Fig. 4SYBR Green real-time PCR analysis of TGF ...
Amplification of DNA is exponential in the early and middle cycles of a PCR (i.e. it is linear on a logarithmic scale). This property can be exploited to infer the starting amount of PCR template (see diagram in Hunt tutorial). During the exponential or log phase each copy of DNA is being amplified, and thus can be a better measure than in endpoint PCR, where reagents such a nucleotides may become exhausted and result in inefficient amplification, resulting in inaccurate quantification of the gene of interest. Real-time PCR is more precise than previously used reverse transcription PCR (RT PCR) because the generation of product is continuously monitored during the PCR run (this is where the term real time comes in), rather than at the end of a PCR reaction (endpoint PCR). Generation of product is detected in one of two ways. First, the amount of double stranded DNA in the tube can be measured using fluorescent dyes which intercalcate double-stranded DNA (like the DNA binding dye SYBR Green ...
The Target Lipid Model (TLM) provides a framework for deriving predicted no effect concentrations (PNEC) for non polar organic chemicals to organisms in the environment. This approach has been used to perform environmental risk assessment of individual hydrocarbons as well as complex petroleum substances. The TLM is based primarily on data for aquatic test organisms and this work evaluatesthe potential for extending the TLM to soil and sediment using Equilibrium Partitioning (EqP) theory.. Literature data for other nonpolar organics were compiled for acute and chronic exposures to invertebrates in soils and sediments. New data were generated according to OECD guidelines (CONCAWE, 2011 and 2012) to evaluate soil and sediment dwelling organisms and to test potential toxicity cut-offs for high log Kow compounds. The default TLM was applied to these data using EqP to develop critical target lipid body burdens (CTLBB) including associated uncertainty in the model application.. Comparison of the ...
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As-mineral-humic substance interactions [Elektronische Ressource] : influence of natural organic matter on sorption and mobility of As / vorgelegt von Prasesh Sharma : As-mineral-humic substance interactions - Influence of natural organic matter on sorption and mobility of As Dissertation zur Erlangung des Grades eines Doktors der Naturwissenschaften der Geowissenschaftlichen Fakultät der Eberhard Karls Universität Tübingen vorgelegt von Prasesh Sharma Kathmandu, Nepal 2010 Tag der mündlichen
Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR, Sekaran Muniandy, Koon Chu Yaiw, Jack Bee Chook,
The 2016-2021 World Outlook for Mixed Synthetic Organic Chemicals for Use As Flavor and Perfume Materials The 2016-2021 World Outlook for Mixed Synthetic Organic Chemicals - Market research report and industry analysis - 9128739
Slovenia Imports of Organic chemicals was US$867.32 Million during 2018, according to the United Nations COMTRADE database on international trade. Slovenia Imports of Organic chemicals - data, historical chart and statistics - was last updated on February of 2020.
DNA microarray technology is a high throughput technique by which the expression of the whole genome is studied in a single experiment. In dual label experiments the fluorescent dyes Cy 3 and Cy 5 are used to label the two RNA samples co-hybridized on a same array. Recently two more dyes have been proposed (Alexa 488 and Alexa 594) allowing the simultaneous hybridization of three or four samples. Forster et al. [2] have evaluated triple-target microarray by comparing results of single-target, dual-target and triple-target microarrays. They have concluded that the use of triple-target microarray is valid from an experimental point of view. One year later, Staal et al. [7] have investigated the four-target microarray experiments. Their approach differs from that of [2], but their conclusions are in fair agreement. Their study has shown that Alexa 594 is best suited as a third dye and that Alexa 488 can be applied as a fourth dye on some microarray types. These extensions of the microarray ...
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Natural organic matter (NOM) represents a complex molecular system that cannot be fully characterized compositionally or structurally in full atomistic detail. This makes the application of molecular modeling approaches very difficult and significantly hinders quantitative investigation of NOM properties and behavior by these otherwise very efficient computational techniques. Here we report and analyze three molecular dynamics (MD) simulations of Ca2+ complexation with NOM in aqueous solutions in an attempt to quantitatively assess possible effects of model- and system size-dependence in such simulations. Despite some obvious variations in the computed results that depend on the size of the simulated system and on the parameters of the force field models used, all three simulations are quite robust and consistent. They show Ca2+ ions associated with 35-50% of the NOM carboxylic groups at near-neutral pH and point to a strong preference for the stability of bidentate-coordinated contact ion pairs. The
Natural organic matter (NOM) from seawater is a complex mixture of compounds that has only been roughly characterized. In this study, advanced techniques such a
Introduction: Propionibacteriaceae (Propioni) are anaerobic bacteria associated with human and animal infections. Todays methods of diagnosis for Propioni are unsatisfactory due to lack of sensitivity of culture, time required for culture results (3 to 14 days) and difficulties for interpretation of SYBR Green real-time PCR results. The goal of this work was to validate a new rapid and sensitive test for the diagnosis of Propioni infections (endophthalmitis, corneal ulcers and others).. Material and methods: DNA was extracted using the MagNA Pure® isolation kit (Roche) and bacterial detection and quantification was carried out with a set of original primers and probe (5ATACGTAGGGTGCGAGCGTTGTCC; 5TGGTGTTCCTCCTGATATCTGCGC and [Amino C6+JOE]-GATCGCGTCGGAAGTGTAATCTTGGGG-Black Hole Quencher). PCR cycling program consisted in one cycle at 95°C, 20 sec and 45 cycles at 95°C, 3 sec and 30 sec at 60°C. DNA extraction yields were assessed in the same tube.. Results: This test detects as few as 0.01 ...
Galeano, Luis-Alejandro Y Luna, Crhistian Y Vicente, M. Angel Y Bravo, P.F Y Gil, Antonio Y Rosero, M. Y Delgado, S. Ximena Y Rodriguez, G. (2011) Removal of natural organic matter from a surface feedstock for drinking water production by Al/Fe-PILC - catalyzed wet peroxide oxidation: Effect of catalyst preparation from concentrated precursors. In: IWA Specialist Conference, Mayo, 2011, Valladolid, España.. ...
C. E. Gerwe, Lawler, D. F., Speitel Jr, G. E., Katz, L. E., and Bennett, P. C., Natural Organic Matter (NOM) Adsorption onto and Coprecipitation with Solids Formed during Softening, Annual Conference Proceedings. American Water Works Association, 2003 ...
This report clearly demonstrates the feasibility of a fluorescence-based assay for high-throughput screening of antimalarials. The results of this malaria SYBR Green I-based fluorescence (MSF) assay were in agreement with those of the [3H]ethanoloamine assay, and the level of fluorescence was shown to have a direct, linear correlation with the level of parasitemia. The drugs tested in this study are varied in their structures, mechanistic actions, and potencies; and the range of parasitemia tested for fluorescence linearity includes the entire realistic range encountered in a 48-h assay under our starting conditions. On the basis of this side-by-side comparison, these observations suggest that the assay will be broadly applicable, and that has been our experience. In fact, in our laboratory the MSF assay has replaced assays that use radioisotopes and has been used to perform several hundred assays involving a wide range of experimental conditions.. The determination of EC50s by the MSF assay ...
Method 525.3 was developed by the US EPA for the determination of semi-volatile organic chemicals in drinking water. This is an analytical method that uses solid phase extraction (SPE) and gas chromatography/mass spectrometry (GC/MS) for the identification and quantitation of 123 selected compounds. These compounds range from polychlorinated biphenyls (PCBs), poly aromatic hydrocarbons (PAHs), and pesticides. The standards can be purchased individually or as a complete set for your laboratorys particular needs. Additionally, the required surrogate and internal standards are also offered.
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Genotoxic compounds form covalently bound adducts with DNA, a process which could lead to mutagenesis and carcinogenesis. In this dissertation, methods that can be used to screen organic chemicals for genotoxicity were developed. The first method employs DNA-polyion films, neutral thermal hydrolysis of these films, and liquid chromatography-mass spectrometry (LC-MS) to quantify DNA adducts of molecules that interact directly with DNA. Neutral thermal hydrolysis provides a simple sample preparation procedure for N7-guanine and N3-adenone adducts because these adducts are selectively ejected from the DNA chain, resulting in a clean sample matrix enriched in nucleobase adducts. Coupling neutral thermal hydrolysis with LC-MS provides sensitive methods to detect and quantitate DNA adducts, and structural information is provided by MS. Neutral hydrolysates of DNA-polyion films used in voltammetric genotoxicity screening sensors were analyzed by LC-MS. Results showed that concentrations of N7-guanine DNA
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