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SACAQMD RULE 447 ORGANIC LIQUID LOADING LAST REVISED 11/16/93 SACRAMENTO METROPOLITAN AQMD RULES AND REGULATIONS RULE 447 ORGANIC LIQUID LOADING Adopted 1-14-74 (Amended 11-20-84, 4-18-89, 4-30-91, 11-16-93) INDEX 100 GENERAL 101 PURPOSE 110 EXEMPTION, LOW VAPOR PRESSURE MATERIALS 200 DEFINITIONS 201 BACKGROUND 202 BULK PLANTS 203 BULK TERMINAL 204 EXEMPT COMPOUNDS 205 LEAK FREE 206 LOADING FACILITY 207 VAPOR TIGHT 208 VOLATILE ORGANIC COMPOUNDS 300 STANDARDS 301 VAPOR RECOVERY REQUIRED 302 VAPOR RECOVERY REQUIREMENTS, BULK TERMINALS 303 VAPOR RECOVERY REQUIREMENTS, BULK PLANTS 304 EQUIPMENT MAINTENANCE 305 VAPOR DIAPHRAGM REQUIREMENTS 400 ADMINISTRATIVE REQUIREMENTS 401 COMPLIANCE SCHEDULE 500 MONITORING AND RECORDS 501 TESTING PROCEDURE 502 RECORDS 100 GENERAL 101 PURPOSE: To limit emissions from the loading of organic liquids. 110 EXEMPTION, LOW VAPOR PRESSURE ORGANIC LIQUIDS: The loading of organic liquids having a vapor pressure less than 0.5 psia under actual loading conditions is exempt ...
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Staining agarose gels with SYBR Green I does not interfere with the transfer of nucleic acids to membranes or subsequent hybridization in Southern or Northern blot analysis as long as 0.1% -0.3% SDS is included in prehybridization and hybridization buffers to remove the dye ...
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Fluorescence differential display. FDD was performed according to Ito et al. (1994). In brief, 2.5 μg of DNA-free total RNAs, prepared from cerebella of ICR mice (Nippon SLC, Shizuoka, Japan) at various developmental stages [embryonic day 18 (E18), P0, P3, P7, P12, P15, P21, and 8-week-old], were reverse-transcribed using a Superscript II reverse transcriptase (Life Technologies, Rockville, MD) in the presence of 2.5 μm anchored oligo-dT primers 5′-GT15A-3′. PCR was performed using an ExTaq polymerase kit (Takara Shuzo, Kyoto, Japan), the anchored primer, and arbitrary primers (10-mer), essentially as described by Ito et al. (1994). Clone 13-2 was generated using 5′-GTTTTCGCAG-3′ as an arbitrary primer. The PCR products were separated on a 6% polyacrylamide gel and analyzed by a computerized-fluorescent image analyzer FluorImager (Amersham Pharmacia Biotech, Piscataway, NJ) after staining with the fluorescent dye SYBR Green I (Takara Shuzo). Bands of interest were excised from FDD gels ...
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401 KAR 61:175. Leaks from existing synthetic organic chemical and polymer manufacturing equipment.. RELATES TO: KRS 224.20-100, 224.20-110, 224.10-120, 40 C.F.R. 60 Appendix A (Method 21), 42 U.S.C. 7410. STATUTORY AUTHORITY: KRS 224.10-100. NECESSITY, FUNCTION, AND CONFORMITY: KRS 224.10-100 requires the Environmental and Public Protection Cabinet to prescribe administrative regulations for the prevention, abatement and control of air pollution. 42 USC 7410 likewise requires the state to implement standards for national primary and secondary ambient air quality. This administrative regulation provides for the control of volatile organic compound emissions from leaks from existing synthetic organic chemical and polymer manufacturing equipment.. Section 1. Definitions. As used in this administrative regulation, all terms not defined in this section shall have the meaning given to them in 401 KAR 61:001.. (1) Affected facility means each individual component which contacts a process fluid that ...
401 KAR 61:175. Leaks from existing synthetic organic chemical and polymer manufacturing equipment.. RELATES TO: KRS 224.20-100, 224.20-110, 224.10-120, 40 C.F.R. 60 Appendix A (Method 21), 42 U.S.C. 7410. STATUTORY AUTHORITY: KRS 224.10-100. NECESSITY, FUNCTION, AND CONFORMITY: KRS 224.10-100 requires the Environmental and Public Protection Cabinet to prescribe administrative regulations for the prevention, abatement and control of air pollution. 42 USC 7410 likewise requires the state to implement standards for national primary and secondary ambient air quality. This administrative regulation provides for the control of volatile organic compound emissions from leaks from existing synthetic organic chemical and polymer manufacturing equipment.. Section 1. Definitions. As used in this administrative regulation, all terms not defined in this section shall have the meaning given to them in 401 KAR 61:001.. (1) Affected facility means each individual component which contacts a process fluid that ...
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The annual Synthetic Organic Chemicals (SOC) reports provided information on U.S. production and sales of synthetic organic chemicals and the raw materials from which they are made. Chapter 1 of each report presents more information on the scope of the reports; the authority under which the data were collected; and the method used to collect the data.
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Finland Exports of Organic chemicals was US$996.24 Million during 2018, according to the United Nations COMTRADE database on international trade. Finland Exports of Organic chemicals - data, historical chart and statistics - was last updated on February of 2020.
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Deoxy+ Real-time SYBR Green RT-PCR system provides user with a rapid and simple way to quantify the expression of gene of interest based on Real-time PCR system containing SYBR Green. Yeasterns Deoxy+ HiSpec RT, Hotstart DNA polymerase and all the components for Real-time SYBR Green RT-PCR are skillfully mixed within a single tube. Unique buffer system allows highly specific quantification by preventing the formation of nonspecific products and primer-dimers.. ...
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Our lncRNA gene expression products include sample to insight solutions to enable quick, reliable gene expression analysis. As leaders in the field of high-performance SYBR® Green real-time PCR analysis, our RT2 lncRNA PCR Arrays with comprehensive, easy-to-use data analysis tools deliver focused and accurate results, allowing you to overcome the bottlenecks in your gene expression studies. We provide you with the products that allow you to spend less time at the bench and more time interpreting your results ...
Our lncRNA gene expression products include sample to insight solutions to enable quick, reliable gene expression analysis. As leaders in the field of high-performance SYBR® Green real-time PCR analysis, our RT2 lncRNA PCR Arrays with comprehensive, easy-to-use data analysis tools deliver focused and accurate results, allowing you to overcome the bottlenecks in your gene expression studies. We provide you with the products that allow you to spend less time at the bench and more time interpreting your results ...
Over-expression SDF-1 in AdV.SDF-1 group increased the expression of VEGF and bFGF mRNA analyzed in the myocardium infarction area (Fig. 4) after MI, and VEGF and bFGF expression increased obviously in 3 day after MI and continued until 7 28 days after MI.Fig. 4SYBR Green real-time PCR analysis of TGF ...
Amplification of DNA is exponential in the early and middle cycles of a PCR (i.e. it is linear on a logarithmic scale). This property can be exploited to infer the starting amount of PCR template (see diagram in Hunt tutorial). During the exponential or log phase each copy of DNA is being amplified, and thus can be a better measure than in endpoint PCR, where reagents such a nucleotides may become exhausted and result in inefficient amplification, resulting in inaccurate quantification of the gene of interest. Real-time PCR is more precise than previously used reverse transcription PCR (RT PCR) because the generation of product is continuously monitored during the PCR run (this is where the term "real time" comes in), rather than at the end of a PCR reaction ("endpoint" PCR). Generation of product is detected in one of two ways. First, the amount of double stranded DNA in the tube can be measured using fluorescent dyes which intercalcate double-stranded DNA (like the DNA binding dye SYBR Green ...
The Target Lipid Model (TLM) provides a framework for deriving predicted no effect concentrations (PNEC) for non polar organic chemicals to organisms in the environment. This approach has been used to perform environmental risk assessment of individual hydrocarbons as well as complex petroleum substances. The TLM is based primarily on data for aquatic test organisms and this work evaluatesthe potential for extending the TLM to soil and sediment using Equilibrium Partitioning (EqP) theory.. Literature data for other nonpolar organics were compiled for acute and chronic exposures to invertebrates in soils and sediments. New data were generated according to OECD guidelines (CONCAWE, 2011 and 2012) to evaluate soil and sediment dwelling organisms and to test potential toxicity cut-offs for high log Kow compounds. The default TLM was applied to these data using EqP to develop critical target lipid body burdens (CTLBB) including associated uncertainty in the model application.. Comparison of the ...
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Synthetic Organic Chemical Manufactur-ers Association: Former URS executive Michael C. Nines was named manager of the Responsible Care program.
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Based on our background we have first tackled the questions of equilibrium sorption of organic chemicals to various tissues and organs. In contrast to the often used Kow approach we consider storage lipids and membrane lipids as separate tissues with different sorption properties and we also take into account various proteins such as albumin, muscle proteins and collagen. All these equilibrium partition coefficients are estimated for neutral organic chemicals with our pp-LFER approach (Endo et al, 2013). In contrast, to neutral organic chemicals, sorption processes of ionic species are still not well understood. For sorption of ionic organic species in membrane lipids we have recently presented a promising approach (Bittermann et al., 2014). Sorption of ionic species in storage lipids is assumed to be negligible. In contrast, equilibrium sorption of ionic species to albumin and muscle protein cannot be ignored and is a matter of ongoing research in our group. ...
Perform reliable qPCR with Bio-Rads pre-validated GAS2 (1 of 2) primer pair, for the Zebrafish genome. Designed for SYBR Green-based detection.
Perform reliable qPCR with Bio-Rads pre-validated GAS1 primer pair, for the Cow genome. Designed for SYBR Green-based detection.
SYBR vs FAM probe - posted in PCR, RT-PCR and Real-Time PCR: I will be looking at virus replication by real-time PCR using primers for antigenomic RNA. I originally wanted to do this with a FAM probe for increased sensitivity, but I am having much better luck with SYBR green. I am unfamiliar with what is acceptable for measuring virus replication. If I was to present my data measuring replication by RT-PCR with SYBR green, would reviewers question why I did not use a more sensitive techni...
These retroviral and lentiviral titration kits measure virus titers using SYBR Green-based, real-time PCR. Retrovirus and lentivirus copy numbers are determined by comparing the samples Ct to a standard curve derived from a calibrated control template.
Retroviral and lentiviral titration kits that measure virus titers using SYBR Green-based, real-time PCR. Viral copy numbers are determined by comparing the samples Ct to a standard curve derived from a calibrated control template.
You may also wish to search for items by Jochims, H. and Jochims. 13 matching references were found. Lohr, W.; Jochims, H.-W.; Baumgartel, H., Photoreaktionen kleiner organischer Molekule IV Absorptionsspektren, Photoionen- und Resonanz- photoelektronenspektren von Vinylbromid, Ber. Bunsen-Ges. Phys. Chem., 1975, 79, 901. [all data] Jochims, H.-W.; Lohr, W.; Baumgartel, H., Photoreactions of small organic molecules V. Absorption-, photoion- and resonancephotoelectron-spectra of CF3Cl, CF2Cl2, CFCl3 in the energy range 10-25 eV, Ber. Bunsen-Ges. Phys. Chem., 1976, 80, 130. [all data] Jochims, H.-W.; Lohr, W.; Baumgartel, H., Photoionization mass spectrometry studies of deuterated acetaldehydes CH3CDO and CD3CHO, Chem. Phys. Lett., 1978, 54, 594. [all data] Jochims, H.-W.; Lohr, W.; Baumgartel, H., Photoreactions of small organic molecules. VI. Photoionization processes of difluoroethylenes, Nouv. J. Chim., 1979, 3, 109. [all data] Baumgartel, H.; Jochims, H.-W.; Ruhl, E., Photoionenspektroskopic ...
The TriFECTa® RNAi kit does not include primers or PCR reagents for quantification of gene silencing.. We offer, separately, primer sets targeting human or mouse HPRT for use in SYBR® Green-based qPCR assays to monitor RNAi function following transfection with the HPRT-S1 Positive Control DsiRNA. For other human, mouse, or rat targets, consider using a predesigned PrimeTime® qPCR Assay. For other species, you can easily design a custom PrimeTime qPCR Assay using our free, online PrimerQuest® Tool.. The TriFECTa® Kit includes:. ...
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LightCycler® 480 ResoLight Dye belongs to a new generation of dsDNA-binding dyes that can detect the presence of heteroduplexes formed during PCR (e.g., if the sample is heterozygous for a particular mutation). Its possible applications therefore exceed those of other, more traditional DNA-staining dyes, such as SYBR Green I. LightCycler® 480 ResoLight Dye is not toxic to amplification enzymes. Thus, high concentrations of the dye do not affect the PCR. These high concentrations completely saturate the dsDNA in the sample. Therefore, when dye molecules dissociate from dsDNA during melting, there is only little chance for them to re-bind to other unoccupied sites. This makes the melting process highly homogeneous and the acquired signals very sharp. Under these conditions, even small changes in the melting curve result in subtle, but reproducible changes in fluorescence.. For more information on HRM analysis and application examples, click here.. ...
Can someone share the sequences of a pair of primers that has been SUCCESSFULLY employed for the determination of the GAPDH (glyceraldehyde-3-phosphate dehydrogenase) mRNA level in human cells (HeLa) by the real-time PCR method with the SYBR Green I detection ...
SYBR Green I (SG) is a fluorescent dye applied in various techniques of DNA analysis, including fluorescent staining of electrophoretic gels, quantitative polymerase chain reaction, etc. SG binds selectively to double-stranded DNA via intercalation and minor groove interactions, resulting in a considerable enhancement of fluorescence of the dye. Modification of DNA by partial or full replacement of natural purine nucleobase guanine (G) with its synthetic analog 7-deazaguanine (G*) or its derivatives was shown to cause the SG fluorescence quenching. In this paper, we present a comparative study of interactions of SG with natural DNA fragments and with DNA fragments modified with G* by means of fluorescence and electrochemical methods. Competition between unmodified (forming strongly fluorescent complex with SG) and fully G*-modified (not contributing significantly to overall fluorescence signal) DNA fragments for the dye was studied via changes in the fluorescence intensity. In addition, ...
Easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dyes on the StepOnePlus™ RT-PCR System. 96 WELL LHS SYS DYE CAL PLATECalibration Kit ...
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If you are not 100% satisfied with your purchase, you can either return your order for a full refund or exchange it for something else. You can return or exchange your purchase for up to 14 days from the purchase date. Returned or exchanged products must be in the condition you received them, unused and in the original box and/or packaging.. ...
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This week we talked about different types of organic molecules. This was defiantly the hardest week so far. We learned how to identify different types of organic molecules, identify distinguishing features of each class of molecules and when given a molecule we learned to assign class. The different classes we learned about where amino acids,…
The ability of organic chemicals to cause health effects varies greatly from those that are highly toxic, to those with no known health effect. As with other pollutants, the extent and nature of the health effect will depend on many factors including level of exposure and length of time exposed. Eye and respiratory tract irritation, headaches, dizziness, visual disorders, and memory impairment are among the immediate symptoms that some people have experienced soon after exposure to some organics. At present, not much is known about what health effects occur from the levels of organics usually found in homes. Many organic compounds are known to cause cancer in animals; some are suspected of causing, or are known to cause, cancer in humans. ...
Leading supplier of specialty organic chemicals, intermediates and unique materials for research, development and production applications. Many of our organic compounds are available in sizes ranging from milligram to kilo quantities.
Leading supplier of specialty organic chemicals, intermediates and unique materials for research, development and production applications. Many of our organic compounds are available in sizes ranging from milligram to kilo quantities.
hi i just started using GELSTAR available from Biozym. it is more sensitive than EtBr and is good in staining small fragments i would not see with EtBr. i was told that it stains the DNA-backbone. hope it helps :-) ,,I think its Sybr Green by Molecular Probes, Inc., and available through regards dieter.gerards.uni-koeln.de Dieter Gerards ...
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Proteins are made up of chains of small organic molecules called amino acids such as glycine, alanine, lysine or tryptophan. Amino acids contain an amine functional group, which contains a nitrogen...
But meanwhile, we still cannot design a simple small organic molecule that will bind to a protein involved in a disease, be stable inside the body, show minimum side effects and cure or mitigate the effects of that disease. We are about as far away from this goal as physics was from discovering the Big Bang two hundred years ago, perhaps more. Our cancer drugs are still dirty and most of them cause terrible side effects; we just dont have a good enough scientific understanding of drug behavior and the human body to minimize these effects. Our knowledge of neurological disorders like Alzheimers disease is even more backward. There we dont even know what the exact causes are, let alone how we can mitigate them. We still waste billions of dollars in designing and testing new drugs in a staggering process of attrition that we would be ashamed of had we known something better. And as I mentioned in my series of posts on challenges in drug discovery, even something as simple as getting drugs past ...
I will never forget the day that we received a large envelope from Make-A-Wish Iowa. I quickly read through everything. I was excited to share the great news with Jax that he was getting a wish. I could not wait to see what he would wish for. Make-A-Wish tells you to have fun and enjoy the process…have your child dream big….for ANYTHING. So that is what we did, we said to Jax, "if you could wish for anything in the world what would you want?".. I watched him silently start thinking about it. Almost instantly he looks at us with a sparkle in his eye and says, "Oh, I know. I have the perfect wish." I couldnt wait to hear. Then it comes.. "Im going to wish for a small heart.". Of course, all he really wants is a cure too. As I fought back tears I told him that would be my wish too but thats not how it works. Make-A-Wish cannot heal your heart.. Jax was 10 lbs 2 oz at birth. That is a BIG baby. What we would soon find out was that he had a big heart too. He spent 12 days in neonatal intensive ...
Bio Chemicals consists of a number of different organic chemicals with non-fossil origin and as such they are very environmentally friendly.
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Founded in 1869, Robinson Brothers is one of the UKs largest independent manufacturers of speciality organic chemicals, offering a wide range of custom manufactured intermediates on a commercial scale. ...
R200c, miR205 miR-miR376b, miR381, miR4095p, miR410, miR114 TNBC casesTaqMan qRTPCR (Thermo Fisher Scientific) SYBR green qRTPCR (Qiagen Nv) TaqMan qRTPCR
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I just moved and didnt have the money so i left it so its been green for two weeks now but its thick and green i dont know what to do im taking a sample to leslies to day but reading all your Q and As it sounds like i should just drain the pool and start over ...
not sure where "Organic matter degrades over time unless its part of a living organism" comes from... because its not entirely true. Some organic matter is quite stable, depending on the conditions, and it doesnt "need" to be part of a living cell. This is why we can recover DNA from (dead) fossils that is tens of thousands of years old... and why DNA is a reasonably good choice for molecular storage ...
Atomic storage In the search for ever-smaller storage, a team of scientists at Delft University in the Netherlands built a 1 kilobyte memory where each bit is represented by the position of one single chlorine atom. "In theory, this storage density would allow all books ever created by humans to be written on a single post stamp," said lead scientist Sander Otte. They reached a storage de... » read more ...
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Two different real-time polymerase chain reaction (PCR) detection approaches based on SYBR Green I dye and Taqman probe based assays were developed for the detection and differentiation of infectious bursal disease virus (IBDV) subtypes. Both approaches were able to detect and differentiate IBDV subtypes based on the use of subtype-specific primers or subtype-specific probes where the primers were designed based on single nucleotide polymorphism (SNP) concept. After optimization of the primer combinations and PCR parameters, very virulent-specific primer, IF & IVIR, and classical-specific primer, IF & RCLA were used in the SYBR Green I real-time RT-PCR assay. Plasmid DNA carrying the VP4 gene of the references IBDV strains: very virulent strain UPM94/273 and classical strain D78 were established and used as positive controls in the real time RT-PCR. The developed assay had a dynamic detection limit which spans over 5 log10 concentration range for very virulent and spans over 7 log10 ...
A bentonite-based composite was synthesized by applying thin coatings of natural organic matter onto bentonite particles in aqueous suspension. Natural organic
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TY - JOUR. T1 - Secondary organic aerosol from VOC mixtures in an oxidation flow reactor. AU - Ahlberg, Erik. AU - Falk, John. AU - Eriksson, Axel. AU - Holst, Thomas. AU - Brune, William Henry. AU - Kristensson, Adam. AU - Roldin, Pontus. AU - Svenningsson, Birgitta. PY - 2017/1/1. Y1 - 2017/1/1. N2 - The atmospheric organic aerosol is a tremendously complex system in terms of chemical content. Models generally treat the mixtures as ideal, something which has been questioned owing to model-measurement discrepancies. We used an oxidation flow reactor to produce secondary organic aerosol (SOA) mixtures containing oxidation products of biogenic (α-pinene, myrcene and isoprene) and anthropogenic (m-xylene) volatile organic compounds (VOCs). The resulting volume concentration and chemical composition was measured using a scanning mobility particle sizer (SMPS) and a high-resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS), respectively. The SOA mass yield of the mixtures was compared ...
Disinfection in water treatment has been used to protect public health for over 100 years. Disinfectants are added to inactivate pathogens in the drinking water treatment plant and throughout the distribution system. As an unintended consequence, disinfectants react with natural organic matter (NOM) and other background constituents to form disinfection by-products (DBPs). Each disinfectant produces a different range of DBPs. While toxicity effects of many individual DBPs have been studied, the effect of whole-mixtures of DBPs needs further investigation. The overall goal of this project is to develop and optimize a NOM concentration method using a sequential reverse osmosis(RO)/electrodialysis(ED) treatment system that can be used for toxicity studies. This work focuses on data comparison from two methods of NOM concentration and two sources of sample water, Newmark tap water and a filter effluent from a conventionally-treated surface water treatment plant in Central Illinois, IL. Throughout ...
Water is one of the most important components for our life. Preservation of fresh water is one of the most important issues in sustainable development, which is called water treatment. In water treatment there are two main separated steps, separation and degradation. Many kinds of porous materials including zeolites and porous carbons were used for pollutant separation by adsorption. TiO2 was widely used for photocatalytic degradation of pollutant. Here we report new type of inorganic-organic hybrid porous material, metal-organic framework (MOF), which can adsorb pollutant from water followed by degradation of the pollutant by light irradiation. The MOF comprising porphyrin based ligand has good stability in water unlike most of MOFs. The MOF selectively adsorb a pollutant having smaller size than its pore size, which demonstrates size selective pollutant adsorption property of the MOF. In addition, porphyrin ligands decompose the adsorbed pollutant by visible light irradiation. Therefore, the ...
TY - JOUR. T1 - Evidence of aqueous secondary organic aerosol formation from biogenic emissions in the North American Sonoran Desert. AU - Youn, Jong Sang. AU - Wang, Zhen. AU - Wonaschütz, Anna. AU - Arellano, Avelino F. AU - Betterton, Eric. AU - Sorooshian, Armin. PY - 2013/7/16. Y1 - 2013/7/16. N2 - This study examines the role of aqueous secondary organic aerosol formation in the North American Sonoran Desert as a result of intense solar radiation, enhanced moisture, and biogenic volatile organic compounds (BVOCs). The ratio of water-soluble organic carbon (WSOC) to organic carbon (OC) nearly doubles during the monsoon season relative to other seasons of the year. When normalized by mixing height, the WSOC enhancement during monsoon months relative to preceding dry months (May-June) exceeds that of sulfate by nearly a factor of 10. WSOC:OC and WSOC are most strongly correlated with moisture parameters, temperature, and concentrations of O3 and BVOCs. No positive relationship was identified ...
The primary objective of the study is to assess the safety and the anti-dyskinetic properties of sarizotan at various dosages for neuroleptic-induced TD.. The secondary objective of the study is to assess the effects of sarizotan on cognitive function in patients with neuroleptic-induced TD.. The diagnosis of neuroleptic-induced TD will be confirmed by history and physical examination. Patients will be evaluated at baseline, 2, 4, 8, and 12 weeks. Safety will be assessed by vital signs, ECG, routine blood tests, the ACTH stimulation test, and the clinicians and patients global impression of tolerability.. Procedures:. For efficacy, the primary outcome variable will be changes in the Abnormal Involuntary Movement Scale (AIMS), and a baseline score of ,3 (moderate) will be required for item 8 (Severity of Abnormal Movements). Quantitative evaluation of movements will be carried out in two ways. The first involves a series of instrumental and clinical measures that were developed as a battery ...