Read "Rat cytomegalovirus open reading frame R44 is an early-late gene that encodes a nuclear protein, Archives of Virology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
TY - JOUR. T1 - Human papillomavirus (HPV) types 101 and 103 isolated from cervicovaginal cells lack an E6 open reading frame (ORF) and are related to gamma-papillomaviruses. AU - Chen, Zigui. AU - Schiffman, Mark. AU - Herrero, Rolando. AU - DeSalle, Rob. AU - Burk, Robert D.. PY - 2007/4/10. Y1 - 2007/4/10. N2 - Complete genomes of HPV101 and HPV103 were PCR amplified and cloned from cervicovaginal cells of a 34-year-old female with cervical intraepithelial neoplasia grade 3 (CIN 3) and a 30-year-old female with a normal Pap test, respectively. HPV101 and HPV103 contain 4 early genes (E7, E1, E2, and E4) and 2 late genes (L2 and L1), but both lack the canonical E6 ORF. Pairwise alignment similarity of the L1 ORF nucleotide sequences of HPV101 and HPV103 indicated that they are at least 30% dissimilar to each other and all known PVs. However, similarities of the other ORFs (E7, E1, E2, and L2) indicated that HPV101 and HPV103 are most related to each other. Phylogenetic analyses revealed that ...
Upstream open reading frames (uORFs) can down-regulate the translation of the main open reading frame (mORF) through two broad mechanisms: ribosomal stalling and reducing reinitiation efficiency. In distantly related plants, such as rice and Arabidopsis, it has been found that conserved uORFs are rare in these transcriptomes with approximately 100 loci. It is unclear how prevalent conserved uORFs are in closely related plants. We used a homology-based approach to identify conserved uORFs in five cereals (monocots) that could potentially regulate translation. Our approach used a modified reciprocal best hit method to identify putative orthologous sequences that were then analysed by a comparative R-nomics program called uORFSCAN to find conserved uORFs. This research identified new genes that may be controlled at the level of translation by conserved uORFs. We report that conserved uORFs are rare (|150 loci contain them) in cereal transcriptomes, are generally short (less than 100 nt), highly conserved
TY - JOUR. T1 - Genes within genes. T2 - independent expression of phage T4 intron open reading frames and the genes in which they reside.. AU - Gott, J. M.. AU - Zeeh, A.. AU - Bell-Pedersen, D.. AU - Ehrenman, Karen. AU - Belfort, M.. AU - Shub, D. A.. PY - 1988/1/1. Y1 - 1988/1/1. N2 - The td, nrdB, and sunY introns of bacteriophage T4 each contain a long open reading frame (ORF). These ORFs are preceded by functional T4 late promoters and, in the case of the nrdB intron ORF, a functional middle promoter. Expression of phage-encoded intron ORF-lacZ fusions indicates that these T4 genes are highly regulated. The lack of translation of these ORFs from early pre-mRNAs can be accounted for by the presence of secondary structures that are absent from the late RNAs. Because translation of the intron ORFs could disrupt core structural elements required for pre-mRNA splicing, such regulation may be necessary to allow expression of the genes in which they reside.. AB - The td, nrdB, and sunY introns ...
Background Within the scanning model of translation initiation, reinitiation is a non-canonical mechanism that operates on mRNAs harboring upstream open reading frames. The h subunit of eukaryotic initiation factor 3 (eIF3) boosts translation reinitiation on the uORF-containing mRNA coding for the Arabidopsis bZip transcription factor, AtbZip11, among others. The RPL24B protein of the large ribosomal subunit, which is encoded by SHORT VALVE1, likewise fosters translation of uORF-containing mRNAs, for example mRNAs for auxin response transcription factors (ARFs). Results Here we tested the hypothesis that RPL24B and eIF3h affect translation reinitiation in a similar fashion. First, like eif3h mutants, rpl24b mutants under-translate the AtbZip11 mRNA, and the detailed spectrum of translational defects in rpl24b is remarkably similar to that of eif3h. Second, eif3h mutants display defects in auxin mediated organogenesis and gene expression, similar to rpl24b. Like AtbZip11, the uORF-containing ARF mRNAs
Theres a great guide regarding how to ensure that the automated gene annotator that identified the gene youre working on chose the proper start location for translation. The guide is in Dropbox (you dont need an account in Dropbox to get these, but its handy free cloud storage.) The guide can be downloaded here ...
Flannick J, Thorleifsson G, Beer NL, Jacobs SB, Grarup N, Burtt NP, Mahajan A, Fuchsberger C, Atzmon G, Benediktsson R, Blangero J, Bowden DW, Brandslund I, Brosnan J, Burslem F, Chambers J, Cho YS, Christensen C, Douglas DA, Duggirala R, Dymek Z, Farjoun Y, Fennell T, Fontanillas P, Forsén T, Gabriel S, Glaser B, Gudbjartsson DF, Hanis C, Hansen T, Hreidarsson AB, Hveem K, Ingelsson E, Isomaa B, Johansson S, Jørgensen T, Jørgensen ME, Kathiresan S, Kong A, Kooner J, Kravic J, Laakso M, Lee JY, Lind L, Lindgren CM, Linneberg A, Masson G, Meitinger T, Mohlke KL, Molven A, Morris AP, Potluri S, Rauramaa R, Ribel-Madsen R, Richard AM, Rolph T, Salomaa V, Segrè AV, Skärstrand H, Steinthorsdottir V, Stringham HM, Sulem P, Tai ES, Teo YY, Teslovich T, Thorsteinsdottir U, Trimmer JK, Tuomi T, Tuomilehto J, Vaziri-Sani F, Voight BF, Wilson JG, Boehnke M, McCarthy MI, Njølstad PR, Pedersen O, Groop L, Cox DR, Stefansson K, Altshuler D. Loss-of-function mutations in SLC30A8 protect against type 2 ...
Research groupsGene regulation and morphogenesis Genetics and functional genomics of genes with small open reading frames Dr Juan Pablo ..
Research groupsGene regulation and morphogenesis Genetics and functional genomics of genes with small open reading frames Dr Juan Pablo ..
Genome map of SSV1. Open reading frames are shown as block arrows and labeled as in Palm et al. (1991). Virus structural protein genes (Reiter et al., 1987a) an
Codons consist of six bases and there are six reading frames for each DNA strand, so ... so researchers must evaluate six open reading frames.
(KudoZ) English to Polish translation of complete open reading frame of a cDNA molecule: otwarta ramka odczytu (cząsteczki cDNA) [Medical].
Stern-Ginossar and the other researchers suspected that existing maps of HCMVs protein-coding potential, based largely on computational methods, were far from complete. So, they began mapping the positions of ribosomes-the cellular organelles in which proteins are synthesized-during an HCMV infection of human fibroblast cells. With the resulting map, Stern-Ginossar and her colleagues discovered templates for hundreds of previously unidentified proteins that were encoded in corresponding DNA segments of the viral genome, known as open reading frames.. Surprisingly, the researchers found that many of these open reading frames encode for exceptionally short protein sequences (fewer than 100 amino acids). And some of the newly identified open reading frames were even hiding inside other open reading frames, they say.. "A key finding of our work is that each of these templates can encode more than one protein," said Annette Michalski from the Max Planck Institute of Biochemistry in Martinsried, ...
Myc-DDK-tagged ORF clone of Homo sapiens chromosome 5 open reading frame 13 (C5orf13), transcript variant 3 as transfection-ready DNA - 10 µg - OriGene - cdna clones
Myc-DDK-tagged ORF clone of Homo sapiens chromosome 16 open reading frame 52 (C16orf52) as transfection-ready DNA - 10 µg - OriGene - cdna clones
TY - JOUR. T1 - Yeast CAL1 is a structural and functional homologue to the DPR1 (RAM) gene involved in ras processing. AU - Ohya, Yoshikazu. AU - Goebl, Mark. AU - Goodman, Laurie E.. AU - Petersen-Bjørn, Sara. AU - Friesen, James D.. AU - Tamanoi, Fuyuhiko. AU - Anraku, Yasuhiro. PY - 1991. Y1 - 1991. N2 - A 2.3-kilobase pair DNA fragment of the yeast CAL1 gene was cloned by complementation of the cal1-1 mutation, which causes a defect in nuclear division and bud formation (Ohya, Y., Ohsumi, Y., and Anraku, Y. (1984) Mol. & Gen. Genet. 193, 389-394). Nucleotide sequencing of this fragment revealed a single open reading frame (ORF) encoding a polypeptide of 376 amino acids. Comparative analysis of the predicted amino acid sequence has shown that the CAL1 product has similarity to two yeast proteins: the DPR1 (RAM) gene product that is involved in processing of ras protein at the farnesylation step, and the essential ORF2 protein whose structural gene has a head-to-head arrangement with PRP4, ...
The BY4741 strain used in (Nagalakshmi et al. 2008) was used in this study. RNA-Seq was performed using the protocol developed in (Nagalakshmi et al. 2008), further described in (Nagalakshmi et al. 2010) and (Waern et al. 2011), and using the modifications developed by (Parkhomchuk et al. 2009) to generate strand-specific reads.. Analysis was performed on custom software developed in-house using BowTie (Langmead et al. 2009) to map reads to the S288C reference genome available on SGD, downloaded on May 17, 2010. Python, NumPy, SciPy, and matplotlib were used to further process the data. The softwares source code is available (Saccharomyces Genome Database). Of note, to maximize the information gleaned, unmappable reads were trimmed by four bases from the 3′ end and remapping was attempted; this was done iteratively until only 28 bp remained, at which point the read was considered unmappable. This end trimming typically doubled or more the number of mappable reads.. Expression levels were ...
Sorts alternative splicing (AS) and discovers coding potential. spliceR simplifies downstream sequence analysis by allowing annotation of genomic coordinates of the differentially spliced elements. It is able to detect coding potential of transcripts, determines untranslated region (UTR) and open reading frame (ORF) lengths and predicts whether transcripts are nonsense mediated decay (NMD)-sensitive based on compatible annotated start codon positions and their downstream ORF.
C20orf203 is a de novo human gene that evolved from non-coding DNA after the split between human and chimp. In the human lineage 5 point mutations in the region resulted in the creation of an open reading frame which lead to the expression and translation of 194 amino acid (22kDa) protein. This protein is enriched in the brain and is up regulated in the brains of Alzheimers patients and thus has been hypothesized to play a role in the pathogenesis of this disease.. ...
Abstract: Narnaviruses have been described as positive-sense RNA viruses with a remarkably simple genome of ~3 kb, encoding only a highly conserved RNA-dependent RNA polymerase (RdRp). Many narnaviruses, however, are ambigrammatic and harbour an additional uninterrupted open reading frame (ORF) covering almost the entire length of the reverse complement strand. No function has been described for this ORF, yet the absence of stops is conserved across diverse narnaviruses, and in every case the codons in the reverse ORF and the RdRp are aligned. The >3 kb ORF overlap on opposite strands, unprecedented among RNA viruses, motivates an exploration of the constraints imposed or alleviated by the codon alignment. Here, we show that only when the codon frames are aligned can all stop codons be eliminated from the reverse strand by synonymous single-nucleotide substitutions in the RdRp gene, suggesting a mechanism for de novo gene creation within a strongly conserved amino-acid sequence. It will be ...
Buy C6orf125 elisa kit, Human chromosome 6 open reading frame 125 ELISA Kit-NP_115716.1 (MBS9339415) product datasheet at MyBioSource, ELISA Kits
Buy C2orf84 elisa kit, Human chromosome 2 open reading frame 84 ELISA Kit-NP_001035800.1 (MBS9316956) product datasheet at MyBioSource, ELISA Kits
Recombinant Chromosome 10 Open Reading Frame 33 (C10ORF33) Protein (His tag). Spezies: Ratte (Rattus). Quelle: Hefe. Jetzt Produkt ABIN1635561 bestellen.
The structures of both eukaryotic and prokaryotic genes involve several nested sequence elements. Each element has a specific function in the multi-step process of gene expression. The sequences and lengths of these elements vary, but the same general functions are present in most genes.[2] Although DNA is a double-stranded molecule, typically only one of the strands encodes information that the RNA polymerase reads to produce protein-coding mRNA or non-coding RNA. This sense or coding strand, runs in the 5 to 3 direction where the numbers refer to the carbon atoms of the backbones ribose sugar. The open reading frame (ORF) of a gene is therefore usually represented as an arrow indicating the direction in which the sense strand is read.[10] Regulatory sequences are located at the extremities of genes. These sequence regions can be next to the transcribed region (the promoter) or separated by many kilobases (enhancers and silencers).[11] The promoter is located at the 5 end of the gene ...
Predicting open reading frames within genomic sequences is probably one of the most basic yet important hallmarks of bioinformatics and sequencing analysis. This is the process by which, given an organisms genomic sequence or a section of that genomic sequence, we predict what sections of that genome are potential genes. At its most basic level, this can be done by looking for sequence regions between start and stop codons (sequence signals for the beginning and end of a gene). While there are many programs for predicting open reading frames, I often use the common Glimmer3 toolkit. This program works great overall, but one drawback is that it can sometimes be hard to visualize your open reading frames on your genome or genomic region (using Geneious or the Integrated Genomics Viewer) because it does not give you a .gff3 formatted file, which is commonly used by these programs. In this technical post, I am going to focus on the file types you get from Glimmer3, I will explain the .gff3 file ...
... , Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
These plasmids were used in Phoebe Lus paper: Lu PD, Harding HP and Ron D. 2004. Translation re-initiation at alternative open reading frames regulates gene expression in an integrated stress response. J Cell Biol. 167:27-33.. Important Note: These ...
from Bio import SeqIO ,,,record = SeqIO.read("sequence.fasta", "fasta") ,,, table = 1 ,,, min_pro_len = 100 ,,,for strand, nuc in [(+1, record.seq), (-1, record.seq.reverse_complement())]: for frame in range(3): length = 3 * ((len(record)-frame) // 3) #Multiple of three for pro in nuc[frame:frame+length].translate(table).split("*"): if len(pro) ,= min_pro_len: print("%s...%s - length %i, strand %i, frame %i" \ % (pro[:30], pro[-3:], len(pro), strand, frame)) YSDIDQINLNQISNLQRNLKYFITMGDSTG...NNV - length 554, strand 1, frame 2 SSPGDKGHNCKGGSASSLCPHREEHHSHNG...ILT - length 162, strand -1, frame 1 IEHQDSHDDVQPTGYKEGDPPGREGCGTAA...HNW - length 216, strand -1, frame 1 TKVTGNVQATIITPIHVSPCSVVKCEVEKK...SDA - length 122, strand -1, frame 2 ...
Abnova Human EXT1 Full-length ORF (NP_000118.2, 1 a.a. - 746 a.a.) Recombinant Protein with GST-tag at N-terminal 2µg Life Sciences:Protein Biology:Proteins:Proteins
Abnova Human ERAS Full-length ORF (NP_853510.1, 1 a.a. - 233 a.a.) Recombinant Protein with GST-tag at N-terminal 25µg Life Sciences:Protein Biology:Proteins:Proteins
The invention provides antisense antiviral compounds and methods of their use in inhibition of growth of viruses of the picornavirus, calicivirus, togavirus and flavivirus families, as in treatment of a viral infection. The antisense antiviral compounds are substantially uncharged oligomers having a targeting base sequence that is substantially complementary to a viral target sequence which spans the AUG start site of the first open reading frame of the viral genome.
Another common problem, which I encountered again today, is a confusion between "ORFs" and "coding sequences"(or CDSs) in bacterial genomes. An ORF is an open reading frame, i.e. a stretch of nucleotide sequence in a given reading frame that does not contain a stop codon, or in other words, a stretch of sequence within a reading frame bounded by two stop codons. It is NOT the same as a coding sequence (or CDS), which can be defined as a stretch of nucleotide sequence that directly encodes a protein product. CDSs are a feature of protein-coding genes, but simply identifying CDSs does not guarantee that you have found all the genes, as CDSs are not a feature of tRNA, rRNA and small regulatory RNA genes ...
These reference sequences exist independently of genome builds. Explain. These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above. ...
If that sounds like blowing my own trumpet, its not: Im a lowly postdoc, and what I said of myself is probably true of the majority of scientists at or below my level on the food chain. Its also why I am likely to remain a lowly postdoc until I become unemployable in research: those who go on to be PIs and Department Heads and Directors of Institutes are largely those type-A assholes who are willing to cut corners and stomp on other people to get what they want. How exactly am I supposed to give a PI "a hard time"? If I dont, I think its pretty damn clear why not. (You - anyone reading this - can think less of me for that if you wish, but since I doubt that you are one of the rare few who has put their own, and their families, livelihood on the line for a principle, you can also blow me.) I can, and do, discuss these issues with other postdocs - but to what avail? Its precisely the ones who dont listen, who secretly think me naive or weak, who are going to have the competitive edge ...
its synthesis requires a polyamine-induced translation frameshift which links open reading frame 1 (ORF1) with open reading frame 2 (ORF2) ...
Chapter 5: ORFing your DNA sequence (pp. 146-147 in second edition). In the previous section of the course, we were working with DNA sequences from the HIV gp120 envelope protein. Take one of your DNA sequences and follow the instructions to find the open reading frames in the sequence. Since you were working with just a portion of the entire envelope protein, you may get some strange results. Compare your results with the SWISS-PROT entry you found for the protein above to decipher what the output means. Besides the NCBI Open Reading Frame Finder described in the book, ExPASy also has a translation tool you can use, found here ...
Chapter 5: ORFing your DNA sequence (pp. 146-147 in second edition). In the previous section of the course, we were working with DNA sequences from the HIV gp120 envelope protein. Take one of your DNA sequences and follow the instructions to find the open reading frames in the sequence. Since you were working with just a portion of the entire envelope protein, you may get some strange results. Compare your results with the SWISS-PROT entry you found for the protein above to decipher what the output means. Besides the NCBI Open Reading Frame Finder described in the book, ExPASy also has a translation tool you can use, found here ...
Timing of appearance of the difference in the Hominin Lineage as a defined date or a lineage separation event. The point in time associated with lineage separation events may change in the future as the scientific community agrees upon better time estimates. Lineage separation events are defined in 2017 as: ...
2. Help me, lazyweb! I can enter html tables just fine, unless theres an image upstream - then MT inserts a dozen or more ,br, tags above the table! Ive tried ,br clear=all, and every kind of spacing between the table and the character right before it. No amount of text between the table and the image seems to have any effect. My stylesheet is here and you can view source to see the main index, but I cant see any obvious cause of the weirdness ...
1 ATGAATGGTC ATATTTCTAA TCATCCCAGT AGTTTTGGAA TGTACCCATC TCAGATGAAT 61 GGCTACGGAT CATCACCTAC CTTTTCCCAG ACGGACAGAG AACATGGTTC AAAAACAAGT 121 GCAAAGGCCC TTTATGAACA AAGGAAGAAT TATGCACGGG ACAGTGTCAG CAGTGTGTCA 181 GATATATCTC AATACCGTGT TGAACACTTG ACTACCTTTG TCCTGGATCG GAAAGATGCT 241 ATGATCACTG TTGATGATGG AATAAGGAAA TTGAAATTGC TTGATGCCAA GGGCAAAGTG 301 TGGACTCAAG ATATGATTCT TCAAGTGGAT GACAGAGCTG TGAGCCTGAT TGATTTAGAA 361 TCAAAGAATG AACTGGAGAA TTTTCCTTTA AACACAATCC AGCACTGCCA AGCTGTGATG 421 CATTCATGCA GCTATGATTC AGTTCTTGCA CTGGTGTGCA AAGAGCCAAC CCAGAACAAG 481 CCAGATCTTC ATCTCTTCCA GTGTGATGAG GTTAAGGCAA ACCTAATTAG TGAAGATATT 541 GAAAGTGCAA TCAGTGACAG TAAAGGAGGG AAACAGAAGA GGCGGCCCGA CGCCCTGAGG 601 ATGATTTCCA ATGCAGACCC TAGTATACCG CCTCCACCCA GAGCTCCTGC CCCTGCGCCC 661 CCTGGGACCG TCACCCAGGT GGATGTTAGA AGTCGAGTGG CAGCCTGGTC TGCATGGGCA 721 GCCGACCAAG GGGACTTTGA GAAACCAAGG CAGTATCATG AGCAGGAAGA AACACCTGAG 781 ATGATGGCAG CCCGCATTGA CAGAGATGTG CAAATCTTAA ACCACATTTT GGATGACATT 841 GAATTTTTTA TCACAAAACT ...
1 ATGTGCAATA CCAACATGTC TGTACCTACT GATGGTGCTG TAACCACCTC ACAGATTCCA 61 GCTTCGGAAC AAGAGACCCT GGTTAGACCA AAGCCATTGC TTTTGAAGTT ATTAAAGTCT 121 GTTGGTGCAC AAAAAGACAC TTATACTATG AAAGAGGTTC TTTTTTATCT TGGCCAGTAT 181 ATTATGACTA AACGATTATA TGATGAGAAG CAACAACATA TTGTATATTG TTCAAATGAT 241 CTTCTAGGAG ATTTGTTTGG CGTGCCAAGC TTCTCTGTGA AAGAGCACAG GAAAATATAT 301 ACCATGATCT ACAGGAACTT GGTAGTAGTC AATCAGCAGG AATCATCGGA CTCAGGTACA 361 TCTGTGAGTG AGAACAGGTG TCACCTTGAA GGTGGGAGTG ATCAAAAGGA CCTTGTACAA 421 GAGCTTCAGG AAGAGAAACC TTCATCTTCA CATTTGGTTT CTAGACCATC TACCTCATCT 481 AGAAGGAGAG CAATTAGTGA GACAGAAGAA AATTCAGATG AATTATCTGG TGAACGACAA 541 AGAAAACGCC ACAAATCTGA TAGTATTTCC CTTTCCTTTG ATGAAAGCCT GGCTCTGTGT 601 GTAATAAGGG AGATATGTTG TGAAAGAAGC AGTAGCAGTG AATCTACAGG GACGCCATCG 661 AATCCGGATC TTGATGCTGG TGTAAGTGAA CATTCAGGTG ATTGGTTGGA TCAGGATTCA 721 GTTTCAGATC AGTTTAGTGT AGAATTTGAA GTTGAATCTC TCGACTCAGA AGATTATAGC 781 CTTAGTGAAG AAGGACAAGA ACTCTCAGAT GAAGATGATG AGGTATATCA AGTTACTGTG 841 TATCAGGCAG GGGAGAGTGA ...
I am a PhD candidate in the Turner lab. We are interested in searching for novel small open reading frames (smORFs) and smORFencoded bioactive polypeptides in the immune system. We are building a computational pipeline to detect smORFs using NGS technology (e.g. ribosome profiling and RNAseq).. Previously I worked as a bioinformatics developer in the Micklem lab (http://www.micklemlab.org) in the Department of Genetics Cambridge, and was involved in InterMine (http://intermine.org/) project and modENCODE (http://www.modencode.org/) project.. fterwards, I moved to the Ouwehand lab in the Platelet Biology Group, Department of Haematology Cambridge as a bioinformatician. I was involved in Genomics England 100,000 Genomes Project for which we developed a NGS pipeline to process whole genome sequncing data and ThromboGenomics project as the main bioinformatics analyst.. I am interested in bioinformatics and statistical learning. I hold a MSc in Machine Learning and Bioinformatics from the University ...
A frame member for housing a battery cell, frame assembly and frame/battery cell assembly made therefrom, as well as respective methods of making the same, are disclosed. A frame member for housing a battery cell includes an integral frame having a peripheral wall, the peripheral wall having an attachment face and an opposed cooling face, and an attachment feature located on the attachment face and a complementary attachment feature located on the attachment face that is symmetrically opposed to the attachment feature about a plane of symmetry of the frame, the peripheral wall defining an opening that is configured to matingly receive a battery cell therein. A pair of frame members having a cooling member placed therebetween and attached to the respective attachment faces comprises a frame assembly. A frame/battery cell assembly includes a pair of frame assemblies having at least one battery cell placed therebetween.
Meyers et al. started their analysis by manually reannotating all previously identified NBS-LRR genes and searching the genome sequence for genes missed in the earlier annotations. An important outcome of this work was a change in annotation of some 36% of previously identified NBS-LRR genes, suggesting the weakness of current automated annotation methods. This work also identified 11 new pseudogenes, which tend to be annotated as full-length open reading frames by automated methods that simply create introns surrounding frameshift or nonsense mutations. With corrected annotations in hand, Meyers et al. proceeded to catalog the conserved domains of all of the NBS-LRR proteins and conducted phylogenetic analyses to ascertain their relationships.. NBS-LRR proteins can be subdivided into proteins that contain a Toll/Interleukin-1 receptor-like (TIR) region and those that contain a coiled-coil (CC) region near the N terminus. Meyers et al. found 51 CC-NBS-LRR-encoding (CNL) genes and 92 ...
ORFeome Collaboration Clones are fully-sequenced human open reading frames (ORFs) subcloned into Gateway-entry vectors and are available with or without native stop codons.
BLASTX 2.0a19MP-WashU [05-Feb-1998] [Build sol2.5-ultra 01:47:30 05-Feb-1998] Reference: Gish, Warren (1994-1997). unpublished. Gish, Warren and David J. States (1993). Identification of protein coding regions by database similarity search. Nat. Genet. 3:266-72. Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames. Query= CcruzaQ_941 (635 letters) Translating both strands of query sequence in all 6 reading frames Database: /usr/local/db/others/blast/nrdb 322,836 sequences; 97,797,364 total letters. Searching....10....20....30....40....50....60....70....80....90....100% done Smallest Sum Reading High Probability Sequences producing High-scoring Segment Pairs: Frame Score P(N) N A gnl,PID,d1010075 (D50806) elongation factor 2 [Try... +1 1019 4.0e-102 1 A gnl,PID,d1005333 (D21259) elongation factor 2 [Ent... +1 682 2.1e-66 1 A ...
BLASTX 2.0a19MP-WashU [05-Feb-1998] [Build sol2.5-ultra 01:47:30 05-Feb-1998] Reference: Gish, Warren (1994-1997). unpublished. Gish, Warren and David J. States (1993). Identification of protein coding regions by database similarity search. Nat. Genet. 3:266-72. Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames. Query= CShisMan2_386 (721 letters) Translating both strands of query sequence in all 6 reading frames Database: /usr/local/db/others/blast/nrdb 322,836 sequences; 97,797,364 total letters. Searching....10....20....30....40....50....60....70....80....90....100% done Smallest Sum Reading High Probability Sequences producing High-scoring Segment Pairs: Frame Score P(N) N A sp,P16463,F801_SCHMA FEMALE SPECIFIC 800 PROTEIN (FS80... +2 153 5.7e-09 1 A pir,,S27819 vitelline B1 precursor - liver fl... +2 113 0.0012 1 A ...
This track shows alignments of human clones from the ORFeome Collaboration. The goal of the project is to be an unrestricted source of fully sequence-validated full-ORF human cDNA clones in a format allowing easy transfer of the ORF sequences into virtually any type of expression vector. A major goal is to provide at least one fully-sequenced full-ORF clone for each human gene. This track is updated automatically as new clones become available. ...
C19orf48 - C19orf48 (untagged)-Human chromosome 19 open reading frame 48 (C19orf48) available for purchase from OriGene - Your Gene Company.
L1 / ORF2, 0.1 mg. The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies.
Complete information for C8orf49 gene (RNA Gene), Chromosome 8 Open Reading Frame 49, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
C8orf59 overexpression lysate, 0.1 mg. Transient overexpression lysate of chromosome 8 open reading frame 59 (C8orf59), transcript variant 2
C11ORF57 antibody, Internal (chromosome 11 open reading frame 57) for WB. Anti-C11ORF57 pAb (GTX44966) is tested in Human samples. 100% Ab-Assurance.
C1orf85 antibody (chromosome 1 open reading frame 85) for ELISA, ICC/IF, IHC-F, IHC-P, IP, WB. Anti-C1orf85 pAb (GTX51438) is tested in Human samples. 100% Ab-Assurance.
Complete information for C19orf53 gene (Protein Coding), Chromosome 19 Open Reading Frame 53, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Recombinant protein of human chromosome 12 open reading frame 73 (C12orf73), 20 ug available for purchase from OriGene - Your Gene Company.
Gene target information for C3orf79 - chromosome 3 open reading frame 79 (human). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
... A test of a single-story, single-bay frame was performed as part of a program to investigate the behavior of multistory frames. The frame consisted of two ASTM-A441 columns rigidly connected to an ASTM-A36 beam and was subjected to combined vertical and horizontal loads. The frame was so designed that the effect of secondary moments produced by axial forces acting through sway displacements could be observed experimentally. The test also furnished important information concerning the behavior of the high-strength steel columns. The experimental results are compared with predicted values obtained from an elastic-plastic analysis, which accounts for the secondary moments in the frame, the axial loads in the columns, and the increased strength provided by the beam-to-column connections. In addition, in predicting the post-mechanism behavior, the influence of strain hardening is included in a rational, yet simple manner. The theory developed closely
0067] The second frame 142b may connect the first frame 142a to the first rotating unit 141, and enable the first frame 142a to move along a desired path ml (that may or may not be predetermined). For example, the second frame 142b may include a rolling element. The rolling element may be a ball or a roller. The first rotating unit 141 may include a rail corresponding to the rolling element of the second frame 142b. In this case, the rolling element of the second frame 142b may move in a desired direction (that may or may not be predetermined) along the rail formed on the first rotating unit 141. If the rolling element moves, the second frame 142b may move to correspond to the movement of the rolling element. Since the second frame 142b is coupled with the first frame 142a, the first frame 142a may also move according to the movement of the second frame 142b. As a result, the radiation irradiating module 110 and the radiation detecting module 120 may also move along the desired path ml (that may ...
Daily News How Gaining and Losing Weight Affects the Body Millions of measurements from 23 people who consumed extra calories every day for a month reveal changes in proteins, metabolites, and gut microbiota that accompany shifts in body mass.. ...
In chapter 3, "The Sense of Sensibility," author Wendy Jones uses scenes from one of Jane Austens most celebrated novels to illustrate the functioning of the bodys stress response system.. 0 Comments. ...
The obsolete HTML No Frames or frame fallback element, noframes, provides content to be presented in browsers that dont support (or have disabled support for) the frame element.
Screenshot - BYclouder Pandigital Digital Frame Data Recovery - The main function is recovery lost files from Pandigital Digital Frame. Pandigital Digital Frame...
Screenshot - BYclouder Toshiba Digital Frame Data Recovery - The main function is recovery lost files from Toshiba Digital Frame. Toshiba Digital Frame Data...
Double-clicking on a word (or selecting a word or multiple words using keys) in the editor when posting/editing a message now opens a small pop-up where you can select bold, italics, unordered list, ordered list or linking. If linking is selected, a separate window opens up where you can enter the URL (address). Read more & discuss in the About Nikonians forum. ...
Jeep Tj Soft Top Frame - 28 images - 55003 01 Bestop Factory Style Bow Kit 04 06 Wrangler, Soft Tops Canvas Rage Rpg 69999 Rage, Bestop 174 Jeep Wrangler 2007 2016 Replacement Bows And, Jeep Soft Tops And Door Panels Skins Free Shipping Page 1, Jeep Soft Top Frame Ebay
Top 10 Ways to Improve Your Pull Ups: P90x Pull Up Bar Door Frame Requirements. Great Pull Up Bars, Great pull up bars and tips to use them efficiently.
After a surge of big air and big trails becoming a more prolific stance in BMX the guys at United thought it would make perfect sense to design up a big booster. Australian connect Tyson Jones-Peni and Epsom trail boss Freddie Househam did the numbers and came up with the dimensions for the Knightsbridge frame from Uni
There is a brand new micro-site from the Frames for their new record Burn The Maps out today on Anti-! To see the site including streaming and downloadable tracks, tourdates and more go HERE!
IKEA KNOPPÄNG Frame White stained 10x15 cm Can be used hanging or standing, both horizontally and vertically, to fit in the space available.
Bollinger bands range with adx filter ea #### MULTI TIME FRAME TRADING STRATEGY Building a gap trading strategy #### Momentum trading strategies in forex
Add a decorative flair to your photos with the Black Arch 8-Opening Collage Frame! It features scroll accents that add a touch of elegance to your pictures.
What Is Micro-Stuttering? Lets recap how successive frames are generated. Since the ideal perfect balance isnt achievable in the real world and the system cannot render, say, 30 frames in advance, the following scenario is typical: ...
Define frame: to construct by fitting and uniting the parts of the skeleton of (a structure); plan, contrive; shape, construct - frame in a sentence
Where is Action Center in Windows 10? How to open & use Windows 10 Action Center? What to do if it wont open or is not working? All this and more here.
Translation of the polycistronic 35S RNA of CaMV (cauliflower mosaic virus) occurs via a reinitiation mechanism, which requires TAV (transactivator/viroplasmin). To allow translation reinitiation of the major open reading frames on the polycistronic RNA, TAV interacts with the host translational machinery via eIF3 (eukaryotic initiation factor 3) and the 60S ribosome. Accumulation of TAV and eIF3 in the polysomal fraction isolated from CaMV-infected cells suggested that TAV prevents loss of eIF3 from the translating ribosomes during the first initiation event. The TAV-eIF3-80S complex could be detected in vitro by sucrose-gradient-sedimentation analysis. The question is whether TAV interacts directly with the 48S preinitiation complex or enters polysomes after the first initiation event. eIF4B, a component of the 48S initiation complex, can preclude formation of the TAV-eIF3 complex via competition with TAV for eIF3 binding; the eIF4B- and TAV-binding sites on eIF3g overlap. eIF4B out-competes ...
TY - JOUR. T1 - Inactivation of the open reading frame slr0399 in Synechocystis sp. PCC 6803 functionally complements mutations near the Q(A) niche of photosystem II. A possible role of Slr0399 as a chaperone for quinone binding. AU - Ermakova-Gerdes, Svetlana. AU - Vermaas, Willem. PY - 1999/10/22. Y1 - 1999/10/22. N2 - The Synechocystis sp. PCC 6803 triple mutant D2R8 with V247M/A249T/M329I mutations in the D2 subunit of the photosystem II is impaired in Q(A) function, has an apparently mobile Q(A), and is unable to grow photoautotrophically. Several photoautotrophic pseudorevertants of this mutant have been isolated, each of which retained the original psbDI mutations of D2R8. Using a newly developed mapping technique, the site of the secondary mutations has been located in the open reading frame slr0399. Two different nucleotide substitutions and a deletion of about 60% of slr0399 were each shown to restore photoautotrophy in different pseudorevertants of the mutant D2R8, suggesting that ...
A human cytomegalovirus early gene which possesses three temporally regulated promoters is located in the large unique component of the viral genome between 0.054 and 0.064 map units (C.-P. Chang, C.L. Malone, and M.F. Stinski, J. Virol. 63:281-290, 1989). This gene contains a major open reading frame (ORF) located 233 bases downstream of the cap site of an early unspliced RNA. The major ORF predicts a polypeptide of 17 kilodaltons (kDa) which contains a glycoproteinlike signal and anchor domains as well as potential N-glycosylation sites. Antisera were prepared against synthetic peptides derived from amino acid sequences within the major ORF. The antisera detected a viral glycoprotein of 48 kDa in infected cells and recognized the in vitro-translated 17-kDa protein early-gene product. The viral glycoprotein, designated gp48, was modified by N-linked glycans and possibly O-linked glycans. The synthesis of gp48 occurred in the absence of viral DNA replication but accumulated to the highest levels ...
It has been shown previously, by sequence analysis of the S RNA segment of snowshoe hare (SSH) bunyavirus, that two overlapping open reading frames in the viral complementary sequence code for proteins with molecular weights of 26.8 X 10(3) and 10.5 X 10(3) respectively. In addition to the viral nucleocapsid (N) protein, which is coded by the S RNA, analyses of parental and reassortant bunyavirus-infected cell extracts have shown that the viral S RNA and M RNA species each code for non-structural proteins (NSS and NSM, respectively). In the present report, in vitro translation analyses of the S mRNA species recovered from virus-infected cells indicate that a single size class of mRNA directs the synthesis of N and NSS. Compositional analyses of selected tryptic peptides of N and NSS have provided proof that N is the product of the first open reading frame, and NSS the product of the second.
We have previously described sigma A and sigma B and their structural genes, mysA and mysB, respectively, in Mycobacterium smegmatis. We have now sequenced the corresponding regions in the M. tuberculosis and M. leprae chromosomes, and have found the two homologous genes. The chromosomal linkage and the deduced amino acid (aa) sequences of the two genes show very high similarity in the three species of mycobacteria. We also report the finding of two other open reading frames (ORF) in these clusters. orfX, which has an unknown function, is located between mysA and mysB. The other ORF, located downstream from mysB, encodes a homolog of DtxR, the iron regulatory protein from Corynebacterium diphtheriae (Cd). Doukhan, L; Predich, M; Nair, G; Dussurget, O; Mandic-Mulec, I; Cole, S T; Smith, D R; Smith, I
The E4 region of human adenovirus type 2 is predicted to encode seven proteins as judged from its nucleotide sequence and the pattern of differential splicing of its transcript. Two of the open reading frames (ORFs), ORF1 and ORF2, had been identified as being disrupted in the recently published sequence of the related serotype 5 virus. These ORFs were resequenced and found to be intact in the wt300 strain of adenovirus type 5.
FusionGDB is the Fusion Gene annotation DataBase, aiming to provide a resource or reference for functional annotation of fusion genes in cancer for better therapeutic targets. We first collected 48 117 FGs across pan-cancer from three representative fusion gene resources: the improved database of chimeric transcripts and RNA-seq data (ChiTaRS 3.1), an integrative resource for cancer-associated transcript fusions (TumorFusions), and The Cancer Genome Atlas (TCGA) fusions by Gao et al. For these ~48K FGs, we performed functional annotations including gene assessment across pan-cancer fusion genes, open reading frame (ORF) assignment, and protein domain retention search based on multiple isoform gene structure with multiple break points and finally provided the fusion transcript and amino acid sequences for each break point and gene isoforms. For each fusion partner gene, the user can access multiple annotations such as gene summary, assessment scores of each gene in pan-cancer, biological process ...
From the cloning and characterization of cDNAs, we found that the Epstein-Barr virus (EBV) open reading frame (ORF) BMLF1-BSLF2 coding for the early protein EB2 is present in several mRNAs generated by alternative splicing and expressed in the leftward direction from two promoters PM and PM1. The PM promoter controls the expression of two abundant mRNA species of 1.9 and 2 kilobases (kb), whereas the PM1 promoter controls the expression of at least three mRNAs 3.6, 4.0, and 4.4 kb long. The PM promoter probably overlaps with the PS promoter which controls the transcription of a 3.6-kb mRNA expressed in the rightward direction and containing the ORF BSRF1. Although it increases the amount of chloramphenicol acetyltransferase enzyme expressed from the chimeric pMCAT gene, EB2 is not a promiscuous trans-activator of gene expression and does not positively regulate its own expression from promoter PM. The EB2 activation is not promoter dependent but could possibly act by stabilizing mRNAs and ...
Units 1, 2, and 3 will use high-throughput transcriptome analysis (RNA-seq) based on quantitative next-generation sequencing (NGS) technologies, with the aim to compare the transcript profiles of hyper-/hypo-pathogenic Leishmania strains. Mapping of reads to the L. donovani and L. major reference genomes will help to define (i) the density of reads associated to any open reading frame (ORF), (ii) transcripts generated by alternative trans-splicing events, (iii) the map of initiation sites of parasite-specific poly-cistronic transcription, and (iv) sense and antisense transcript abundance [3]. This analysis will allow revealing transcripts that are exclusively expressed in virulent Leishmania, and will thus allow important new insight into Leishmania pathogenicity mechanisms.. ...
防御素(defensins)是一类在先天性免疫系统中起着重要作用的阳离子抗菌肽。因其在机体抵御病原入侵方面起着重要作用,固命名为防御素。防御素对许多病原微生物有着天然的抵抗活性,包括病毒和细菌。目前,几个跟防御素的转录调控相关的转录因子已经被鉴定,如NF-κB、NF-IL6等。在本论文中,我们主要就青鳉防御素的基因功能及转录调控进行研究。首先,我们运用3′, 5′末端快速扩增法 (Rapid Amplification of cDNA Ends techniques, RACE)在青鳉(Oryzias latipes)眼睛中克隆到一个新的β-防御素基因,命名为OlBD。该基因的cDNA全长480bp,其中5′端和3′端非翻译区(Untranslated region,UTR)分别为68bp和240bp,在3′ 末端还含有一个典型的加尾信号(ATTAA)和26bp的多聚腺嘌呤尾(poly A 尾)。在该cDNA中的189bp 的开放阅读框(open reading frame,ORF) 编码了63个氨基酸(aa)的多肽。Southern blot ...
Bacterial genomes serve as a blueprint in all aspects of biological research, and therefore accurate genome annotation is of paramount importance. However, increasing evidence indicates that currently annotated bacterial genomes have missed many genes encoding small proteins ≤60 aa (Wood et al. 2012; Warren et al. 2010). A small gene, or a small open reading frame (sORF), has previously been defined as one encoding proteins of ≤60 aa (Hemm et al. 2010); or alternatively, it accommodates those up to 100 aa (Andrews and Rothnagel 2014). While small proteins have been increasingly reported for their important cellular roles in bacteria (Alix and Blanc-Potard 2008; Martin et al. 2015; Hobbs et al. 2012), studies on small proteins are limited, partly because many small genes are unannotated in sequenced bacterial genomes (Alix and Blanc-Potard 2009; Storz et al. 2014). Despite much effort made to improve gene annotation, the accurate identification of small genes has been a persistent challenge ...
Gene expression during development is regulated both transcriptionally and translationally; however, relatively few examples of translational regulation are known. On p. 3575, Imai and colleagues describe for the first time how an upstream open reading frame (uORF) mediates translational control during plant development. Loss-of-function mutants of Arabidopsis ACAULIS 5 (ACL5), which encodes spermine synthase, have a dwarf phenotype because of a defect in stem elongation. To find out how ACL5 regulates stem elongation, the researchers isolated a dominant suppressor mutant of the acl5 phenotype - sac51-d. They show that sac51-d disrupts a short uORF of SAC51, which encodes a bHLH transcription factor. Other experiments indicate that this disruption might increase the translation of SAC51. Thus, the researchers suggest that the uORF-encoded protein normally prevents the initiation of SAC51 translation, and that ACL5 acts directly or indirectly (possibly through spermines effects on protein ...
This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which overlaps the first initiation start codon in human. An alternately spliced transcript variant has been found which encodes a shorter isoform. This gene has three pseudogenes; two of them are at different locations of chromosome 17 and one of them is on chromosome 1q32.2. [provided by RefSeq, Sep 2014 ...
Although nonsense mutations have been associated with the skipping of specific constitutively spliced exons in selected genes, notably the fibrillin gene, the basis for this association is unclear. Now, using chimaeric constructs in a model in vivo expression system, premature termination codons are …
A network system in which administrators of frame relay networks can recognize the quality of the ATM network suitably and manage the frame relay networks in accordance with the quality condition of the ATM network. When a frame F is transmitted from a frame relay network FR1 at a transmitting side to an IWF, a frame header in which a transmission number is stored is added to the frame F. Then, the frame F is received by a frame relay network FR2 at a transmitted side through the IWF and the ATM network. In the frame relay network FR2, a difference between the transmission number stored in the frame header of the frame F and a reception condition variable kept in the frame relay network FR2 is calculated, and the difference is stored in a quality information collection table as a number of discarded frames in the ATM network.
The worlds first wiki where authorship really matters. Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts.
The worlds first wiki where authorship really matters. Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts.
sORFs.org datasets. This section provides detailed information about the datasets used. For more information about the dataset processing please consult the INFO page. navigate through the datasets on the left to acquire more information about the corresponding dataset. ...
The objective of this technique is to demonstrate how framesets can be used to group blocks of repeated material. Since most user agents and assistive technology provide a way to navigate from frame to frame, using frames to organize elements can provide a mechanism for easily bypassing blocks of repeated content. If the site uses framesets, organize the blocks of content into separate frames. Make certain that the repeated blocks of content appear in the same frame within the frameset of each Web page. In addition, each frame element must have a title attribute to describe the content of the frame. When frames are properly titled, users can use frame navigation to easily navigate between blocks of content.. This technique is appropriate when framesets are already used to organize the content of the page; other techniques are preferred for pages that are not already using framesets, because many people using assistive technology have trouble with frames . An advisory technique about using ...
2. Make a plan.. "Plans are worthless, but planning is everything." Maybe Eisenhower didnt have your reading life in mind when he said this, but it absolutely applies. This challenge is simple and doable, but it provides the structure you need to approach your reading list with intention in 2017.. As soon as you sign up, start thinking about what you want to read, and when youre going to do it. What do you want your reading life to look like next year? What do you want more of? Planning is everything, even though your plan will likely evolve as the year goes on.. 3. Track your reading.. This is big: make a way to record what you read and what you thought of it. Make it as simple or complex as youd like. Were providing simple printables to track your progress, and Id recommend taking it a step further and tracking all your reading in the coming year.. If you dont use a reading journal, nows the time to start. I use my bullet journal to track my reading these days (and if that interests ...
Toward the end of April, the "summer reading" whispers started. But now its June and school is out or almost over, so today Im shifting gears and thinking about summer reading. Reading is a lot like exercise. You need to do it regularly. When you take a vacation from your workouts, it takes some time to get back to where you were before. If I skip exercising for just ten days, I feel like Im starting over when I get back. The same thing happens on a reading vacation. For kids, that can last three months! Ouch! To help prevent "injury," schools often send home a "reading list" so students can keep "fit" over the summer. There are lots of opinions about the lists, particularly when the list hasnt changed since you were in school. Just know they are singular in their plea: please keep your child reading this summer. It can be tricky finding books that will keep them reading through the summer, especially with one of those stagnant, age-old lists! So whats a parent to do?. ...
User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.9.2.3) Gecko/20100423 Ubuntu/10.04 (lucid) Firefox/3.6.3 Build Identifier: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.9.2.3) Gecko/20100423 Ubuntu/10.04 (lucid) Firefox/3.6.3 In gnome desktop environment, we have AT-SPI which is used by assistive technologies. Currently firefox does support accessibility in linux. Whenever a frame is the currently activated frame, it takes the state active However, when firefox is the currently opened frame it does not assume the state of active. Reproducible: Always Actual Results: Firefox frame does not take the state active when it is the activated frame. Expected Results: Firefox frame should take the state active when it is the activated frame ...
Sommer MH, Zagha E, Serrano OK, Ku CC, Zerboni L, Baiker A, Santos R, Spengler M, Lynch J, Grose C, Ruyechan W, Hay J, Arvin AM. Mutational analysis of the repeated open reading frames, ORFs 63 and 70 and ORFs 64 and 69, of varicella-zoster virus. Journal of Virology. 2001 Sep; 75(17): 8224-8239 ...
The human genome was sequenced a. by sequencing each gene on each chromosome, one at a time. b. using DNA fingerprinting. c. by looking for overlapping regions between sequenced DNA fragments. d. using open reading frames.
between our genes lie vast amounts of DNA much of which appears to regulate the expression of our genes but is not transcribed and translated into a protein product. [More] So even when the complete sequence of a genome is known, it is often difficult to spot particular genes (open reading frames or ORFs). One approach to solving the problem is to examine a transcriptome of the organism. Most commonly this is defined as: All the messenger RNA (mRNA) molecules transcribed from the genome. ...