Figure 1. Intratumoral administration of a STAT3 decoy oligonucleotide abrogates target gene expression in patients with HNSCC. A, schema of phase 0 trial. Biopsies of HNSCC tumors were performed and the tumors were injected with a single dose of a STAT3 decoy oligonucleotide (or saline) followed by tumor resection and analysis of target gene expression in the paired tumor samples. OR, operating room. B, downmodulation of STAT3 target genes in representative HNSCC tumors injected with STAT3 decoy as shown by Western blot analyses. Whole-tumor lysates were prepared from 4 HNSCC tumors before and after injection with the STAT3 decoy enrolled on the first dose tier. Proteins (40 μg) were resolved on a 12.5% SDS-PAGE gel and subjected to immunoblotting with anti-Bcl-XL and cyclin D1 antibody. β-Actin was used as a loading control. C, representative images of immunohistochemical staining for cyclin D1 protein expression before and after injection of STAT3 decoy oligonucleotide or saline from 6 ...

The Oligonucleotide Synthesis report underlines a basic synopsis of the Oligonucleotide Synthesis market that entails classifications, definitions, industry chain structure, and applications. The report provides a detailed overview of major drivers, restraints, challenges, opportunities, current market trends and strategies impacting the global market along with estimates and forecast of revenue and share analysis. The report acknowledges that in these aggressive and speedily developing market settings, the latest marketing data are imperative to ascertain performance and make essential decisions for the profitability and growth of the market.. Get Exclusive Sample Report @ https://www.marketdeeper.com/request-for-sample-report-24571.html. In addition, the research evaluated key market aspects, comprising capacity utilization rate, revenue, price, capacity, growth rate, gross, production, consumption, supply, export, market share, cost, import, gross margin, demand, and much more. The study also ...

The present invention provides methods for synthesis and therapeutic use of DNA and RNA oligonucleotides and analogs. RNA oligonucleotides are synthesized using a small, circular DNA template which lacks an RNA polymerase promoter sequence. The RNA synthesis is performed by combining a circular single-stranded oligonucleotide template with an effective RNA polymerase and at least two types of ribonucleotide triphosphate to form an RNA oligonucleotide multimer comprising multiple copies of the desired RNA oligonucleotide sequence. Preferably, the RNA oligonucleotide multimer is cleaved to produce RNA oligonucleotides having well-defined ends. Preferred RNA oligonucleotide multimers contain ribozymes capable of both cis (autolytic) and trans cleavage.

A 2-N-amidoethyl protected nucleoside analog phosphoramidite of formula (1): ##STR1## is useful in the synthesis of a wide variety of oligonucleotide analogs. Coupling yields with phosphoramidite (1) in solution or solid phase oligonucleotide analog synthesis are high and the 2-N-amidoethyl protecting group can be removed easily under standard conditions.

93119DNAArtificial sequenceSingle strand DNA oligonucleotide 1ttctcccttc tccctctgc 19218DNAArtificial sequenceSingle strand DNA oligonucleotide 2gtggtcccaa gacaatgc 18320DNAArtificial sequenceSingle strand DNA oligonucleotide 3cctgcctctg ttgagactcc 20427DNAArtificial sequenceSingle strand DNA oligonucleotide 4ttctgctaac agtattcttt aatgtga 27521DNAArtificial sequenceSingle strand DNA oligonucleotide 5tgaagagttt gattccgaac g 21618DNAArtificial sequenceSingle strand DNA oligonucleotide 6agccagggta tggctgct 18720DNAArtificial sequenceSingle strand DNA oligonucleotide 7cagcctgact ggacacagaa 20820DNAArtificial sequenceSingle strand DNA oligonucleotide 8atctactgtg cccagcgact 20924DNAArtificial sequenceSingle strand DNA oligonucleotide 9gaaaggatca tctctacttt ctgg 241019DNAArtificial sequenceSingle strand DNA oligonucleotide 10gtggcgagat gctagacag 191124DNAArtificial sequenceSingle strand DNA oligonucleotide 11attttgaact tgagcaggta gttg 241220DNAArtificial sequenceSingle strand DNA oligonucleotide ...

381160DNAArtificialSynthesized Oligonucleotide Sequence 1catccgtcac acctgctctg ctatcacatg cctgctgaag tggtgttggc tcccgtatca 60260DNAArtificialSynthesized Oligonucleotide Sequence 2catccgtcac acctgctctg atcagggaag acgccaacac tggtgttggc tcccgtatca 60362DNAArtificialSynthesized Oligonucleotide Sequence 3catccgtcac acctgctctg gggagggtgg cgcccgtctc ggtggtgttg gctcccgtat 60ca 62462DNAArtificialSynthesized Oligonucleotide Sequence 4catccgtcac acctgctctg ggatagggtc tcgtgctaga tgtggtgttg gctcccgtat 60ca 62562DNAArtificialSynthesized Oligonucleotide Sequence 5catccgtcac acctgctctg gaccggcgct tattcctgct tgtggtgttg gctcccgtat 60ca 62662DNAArtificialSynthesized Oligonucleotide Sequence 6catccgtcac acctgcyctg gagctgatat tggatggtcc ggtggtgttg gctcccgtat 60ca 62762DNAArtificialSynthesized Oligonucleotide Sequence 7catccgtcac acctgcycyg cccagagcag gtgtgacgga tgtggtgttg gctcccgtat 60ca 62862DNAArtificialSynthesized Oligonucleotide Sequence 8catccgtcac acctgcycyg ccggaccatc caatatcagc tgtggtgttg gctcccgtat 60ca ...

New terms in the biopharma realm rarely surprise us but keeping up with the technology and the means of manufacturing can often be challenging. What I really enjoy about ISPE is the way they bring these new technologies to life allowing us to learn from the lessons our peers are going through. One of these emerging realms is the science behind Oligonucleotides.. Oligonucleotides are short (20-25 bases typically) DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, forensics and even treatments. In nature, oligonucleotides are usually found as small RNA molecules that function in the regulation of gene expression (e.g. microRNA), or are degradation intermediates derived from the breakdown of larger nucleic acid molecules. In the human body the application of Antisense Oligonucleotides (AO) show great promise in disease states such as Duchenne Muscular Dystrophy (DMD) and Spinal Muscular Dystrophy (SMD).. These chemicals interact directly with the ...

We have shown that unmodified and uncomplexed siRNA can be delivered to multiple tissues, including tumor xenografts, in vivo by at least two different ways of administration. Systemic delivery via tail vein injections of siRNA designed against POLR2A, a target previously shown suitable for tumor growth inhibition using antisense oligonucleotides (14), resulted in tumor growth inhibition. To further optimize in vivo application of siRNA, we choose to target the exogenously expressed eGFP gene because it allows simple readout for gene-specific knockdown. Knockdown of GFP expression should not affect tumor growth kinetics allowing simultaneous evaluation of RNAi (GFP knockdown) and sequence unrelated effect on tumor growth. In line with tumor growth inhibition due to siPOLR2A, eGFP expression in eGFP-positive MiaPaca-II tumors was significantly lowered after systemic delivery of siRNA directed against eGFP. These results agree with three other independent studies. First, it was shown that ...

Oligonucleotide Synthesis Market report categorize the global market by Product (Reagents & Consumables, Equipment, and Synthesized Oligonucleotides), Application (Research, Therapeutics and Diagnostics), End User & Geography

Synthetic Oligonucleotides. Oligonucleotides were obtained from Integrated DNA Technologies (Coralville, IA) in 96-well format and normalized to 100 μM each. Based on the accompanying mass spectrometer data and information from the supplier, we ascertained that, on average, each 42-mer contained ≈50% truncated species. To purify the oligonucleotides, 10 μl of each of the top-strand (or bottom-strand) oligonucleotides were pooled in two separate pools (top-1 to -130 and bot-131 to -259), dried, and dissolved in 50 μl of water. Twenty microliters of concentrated pool plus 20 μl of formamide was heated to 95°C for 2 min, and 10-μl aliquots were loaded into 1.5-mm × 15-mm slots of a preparative 12% sequencing gel and electrophoresed at 1,300 V for ≈4 h. The bands, which migrated close to the xylene cyanol marker, were visualized with a handheld 254-nm UV lamp and excised. The gel was extruded through a tuberculin syringe into 0.7 ml of TE buffer, frozen at -20°C overnight, eluted at ...

Antisense oligonucleotides are an emerging therapeutic option to treat diseases with known genetic origin. In the age of personalised medicines, antisense oligonucleotides can sometimes be designed to target and bypass or overcome a patients genetic mutation, in particular those lesions that compromise normal pre-mRNA processing. Antisense oligonucleotides can alter gene expression through a variety of mechanisms as determined by the chemistry and antisense oligomer design. Through targeting the pre-mRNA, antisense oligonucleotides can alter splicing and induce a specific spliceoform or disrupt the reading frame, target an RNA transcript for degradation through RNaseH activation, block ribosome initiation of protein translation or disrupt miRNA function. The recent accelerated approval of eteplirsen (renamed Exondys 51™) by the Food and Drug Administration, for the treatment of Duchenne muscular dystrophy, and nusinersen, for the treatment of spinal muscular atrophy, herald a new and exciting era in

Page contains details about telomere complementary oligonucleotide modified gold nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com

Oligonucleotide Synthesis Linkers Market research report has been published by A2Z Market Research to give desired insights to drive the growth of bus

The Business Market Insights provides you regional research analysis on Europe Oligonucleotide Synthesis Market and forecast to 2027. The research report.... ...

Locked Nucleic Acid (LNA) was first described by Wengel and co-workers in 19981 as a novel class of conformationally restricted oligonucleotide analogues. LNA is a bicyclic nucleic acid where a ribonucleoside is linked between the 2-oxygen and the 4-carbon atoms with a methylene unit. Oligonucleotides containing LNA exhibit unprecedented thermal stabilities towards complementary DNA and RNA2, which allows excellent mismatch discrimination. In fact, the high binding affinity of LNA oligos allows for the use of short probes in, for example, SNP genotyping3, allele specific PCR and mRNA sample preparation. LNA is recommended for use in any hybridization assay that requires high specificity and/or reproducibility, e.g., dual labelled probes, in situ hybridization probes, molecular beacons and PCR primers. Furthermore, LNA offers the possibility to adjust Tm values of primers and probes in multiplex assays. LNA can be mixed with DNA and RNA, as well as other nucleic acid analogues, modifiers and ...

Background. Excessive proliferation of pulmonary artery smooth muscle cells is the major pathogenetic factor in vascular remodelling observed in pulmonary hypertension (PH). Our previous results identified the tumour suppressor cyclin-dependent kinase inhibitor (CDKN)1A as a direct target of microRNA (miR)-130, which was found to be upregulated in experimental PH. Here we antagonized miR-130 by LNA-modified seed blockers in vivo.. Methods. To induce experimental PH the hypoxia mouse model (10% oxygen for 5 weeks) was used. LNA-modified seed blockers directed against the miR-130 family (LNA-130) were injected intraperitoneally (2.5mg/kg body weight, after 21d and 28d). Right ventricular hypertrophy was assessed by the ratio of right to left ventricular volume (Fulton index). Tissue expression of CDKN1A was analysed by Western Blotting.. Results. The Fulton index increased in mice under hypoxic conditions as compared to normoxic controls (from 0.25±0.04 to 0.4±0.07, p,0.01). Treatment with ...

Genetic mutations can cause a wide range of diseases, e.g. cancer. Gene therapy has the potential to alleviate or even cure these diseases. One of the many gene therapies developed so far is RNA-cleaving deoxyribozymes, short DNA oligonucleotides that specifically bind to and cleave RNA. Since the development of these synthetic catalytic oligonucleotides, the main way of determining their cleavage kinetics has been through the use of a laborious and error prone gel assay to quantify substrate and product at different time-points. We have developed two new methods for this purpose. The first one includes a fluorescent intercalating dye, PicoGreen, which has an increased fluorescence upon binding double-stranded oligonucleotides; during the course of the reaction the fluorescence intensity will decrease as the RNA is cleaved and dissociates from the deoxyribozyme. A second method was developed based on the common denominator of all nucleases, each cleavage event exposes a single phosphate of the ...

© 2015 Elsevier B.V. Oligonucleotide-based drugs have received considerable attention for their capacity to modulate gene expression very specifically and as a consequence they have found applications in the treatment of many human acquired or genetic diseases. Clinical translation has been often hampered by poor biodistribution, however. Cell-penetrating peptides (CPPs) appear as a possibility to increase the cellular delivery of non-permeant biomolecules such as nucleic acids. This review focuses on CPP-delivery of several classes of oligonucleotides (ONs), namely antisense oligonucleotides, splice switching oligonucleotides (SSOs) and siRNAs. Two main strategies have been used to transport ONs with CPPs: covalent conjugation (which is more appropriate for charge-neutral ON analogues) and non-covalent complexation (which has been used for siRNA delivery essentially). Chemical synthesis, mechanisms of cellular internalization and various applications will be reviewed. A comprehensive coverage of the

RNA and 2 -O-methyl RNA oligonucleotides RNA and 2 -O-methyl RNA oligonucleotide synthesis is performed at Gene Link using the b cyanoethyl chemistry and state of the art synthesizers. These involve proprietary software protocols with long coupling times and specialized cycles to obtain ultra clean oligos.. RNA oligos are susceptible to degradation to the same extent as native RNA extracted from various sources. An attractive alternate to prevent degradation from nucleases is the use of 2 -O- methyl RNA bases, when specific 2 OH is not required. The 2 -O- methyl oligonucleotides confer considerable nuclease resistance and are similar in hydrogen bonding properties to RNA/RNA than the lower RNA/DNA binding property. The coupling efficiency of 2 -O- methyl phosphoramidite is also higher than the RNA monomers resulting in higher yield of full length oligos.. Gene Link also offers custom synthesis of RNA and DNA chimeric oligos with investigator specified ribo or deoxy bases or 2 -O-methyl bases. ...

2′O-Methyl RNA is a nucleic acid analogue that is characterized by the exceptional hybridization properties that it imparts with complimentary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids.The unique combination of properties of 2′O-Methyl RNA has found widespread use in the fields of:Diagnostic probesAptamer and ribozyme developmentMixed 2′O-Methyl-RNA/DNA antisense molecules2′O-Methyl RNA nucleoside can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance. Key features of 2′O-Methyl RNA PhosphoramiditesHigh yield of oligonucleotidesCompatible with DNA synthesisCan be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotidesPurification and other downstream processing of fully modified 2′O

The applications of oligonucleotides are vast, even beyond the life science / pharma sector; however, owing to complexities associated with the synthesis and

The invention relates to immunostimulatory oligonucleotides and methods of using immunostimulatory oligonucleotides to induce an antigen-specific immune response. The invention further relates to a vaccine that comprises an immunostimulatory oligonucleotide and an antigen, and comprises a pharmaceutically acceptable carrier. The immunostimulatory oligonucleotides of the invention, in some embodiments, include one or more modified linkage(s).

Oligonucleotide frequencies were used to compute distances among completely sequenced genomes within a genera and to cluster them. The resulting dendrograms are available by selecting the genera in the right. These comparisons are provided as examples of the clustering power of oligonucleotides and to show the potencial of this method to cluster together related genomes. This method may be used even for typing within same species. The sequenced used in this service were obtained from Release 35 (by EMBL-EBI). This releases includes the latest versions of 44,039 genomes (43,552 bacteria and 494 archaea) from the The International Nucleotide Sequence Database Collaboration (INSDC) archives. Comparison is only available when at least 5 genomes are vailable within the genera or species. Show more ...

Degenerate DNA Oligonucleotides. Our team offers the synthesis of DNA oligonucleotides with equimolar mixtures of two or more different bases at a given position within the sequence. Synthesis scales of 50 nmole and 200 nmole are available. No extra charges apply. -------------------------------------------------------------------------------------------------------------------------------------------. Contact us! Please let us know what your individual research needs are. We can accommodate special requests that might not be explicitly listed here. We also offer free technical assistance to help you make the best choice for your specific application.. For a brief introduction of our DNA Oligonucleotide Synthesis unit and an overview of our products and services, please see here.. ...

Compositions of antisense oligonucleotides conjugated to peptides of a plurality of N-methylpyrrolecarboxamides linked by peptide bonds is provided. The compositions form stable hybridization complexes with DNA and can be used for any purpose which involves hybridizing an oligonucleotide to a DNA molecule, such as in antisense procedures. A method for enhancing oligonucleotide binding to a target is also provided. The method involves the step of hybridizing the target DNA with an oligonucleotide-peptide composition.

Dye-labeled peptides and oligonucleotides are important tools in biochemical and cellular studies. Fluorescent peptides and oligonucleotides have been extensively used in all major types of fluorescence imaging including fluorescence resonance energy transfer (FRET).. Labeling strategies for oligonucleotides (oligos) differ somewhat from those for other commonly encountered biopolymers, such as proteins and carbohydrates, since oligos are exceptionally inert to common bioconjugation reagents under standard reaction conditions.. To assist with overcoming the difficulties in developing these extremely useful biomolecules, multiple materials and resources exist to streamline the process and procedures on both a small and large scale.. ...

Triplex-forming oligonucleotides (TFOs) can bind to polypurine/polypyrimidine regions in DNA in a sequence-specific manner. The specificity of this binding raises the possibility of using triplex formation for directed genome modification, with the ultimate goal of repairing genetic defects in human cells. Several studies have demonstrated that treatment of mammalian cells with TFOs can provoke DNA repair and recombination, in a manner that can be exploited to introduce desired sequence changes. This review will summarize recent advances in this field while also highlighting major obstacles that remain to be overcome before the application of triplex technology to therapeutic gene repair can be achieved.. ...

Incorporation of a piperazino-modified 2-amino-LNA monomer (PipLNA-T) into oligonucleotides conferred very high affinity and base-pairing selectivity towards complementary DNA and RNA strands. Furthermore, one PipLNA-T modification provided a robust nuclease resistance that safeguarded three neighbouring natural nucleosides from 3-exonucleolytic degradation. These favourable properties render PipLNA-T a promising oligonucleotide modification for various biological applications ...

Development of cheap, high-throughput and reliable gene synthesis methods will broadly stimulate progress in biology and biotechnology. Currently, the reliance on column-synthesized oligonucleotides as a source of DNA limits further cost reductions in gene synthesis. Oligonucleotides from DNA microc …

TY - GEN. T1 - Therapeutic applications and mechanisms underlying the activity of immunosuppressive oligonucleotides. AU - Klinman, Dennis M.. AU - Tross, Debbie. AU - Klaschik, Sven. AU - Shirota, Hidekazu. AU - Sato, Takeshi. N1 - Copyright: Copyright 2018 Elsevier B.V., All rights reserved.. PY - 2009/9/1. Y1 - 2009/9/1. N2 - Synthetic oligodeoxynucleotides (ODN) capable of neutralizing or inhibiting immune responses have been described. This review will focus on the properties of phosphorothioate ODN that mimic the immunosuppressive activity of the repetitive TTAGGG motifs present in mammalian telomeres. These TTAGGG multimers block the production of pro-inflammatory and T helper type 1 cytokines elicited when immune cells are activated by a wide variety of Toll-like receptor ligands, polyclonal activators, and antigens. Several mechanisms contribute to the suppressive activity of such ODN. Ongoing microarray studies indicate that suppressive ODN interfere with the phosphorylation of ...

For PCR and qPCR assay planning, SciTools offers two specialized analysis options. PrimerQuest lets the user specify the stretch of sequence to be amplified, along with the desired reaction conditions and the positional sequence constraints the primers need to operate under. The program then identifies primer pairs optimized to these specifications, favoring those pairs that reduce the chances of unwanted primer-dimer formation. For users of IDTs PrimeTime qPCR System, SciTools includes a highly flexible RealTime PCR design tool, specifically developed to simplify the design of qPCR experiments. Users provide a specific gene sequence or reference database accession number and the system automatically generates several optimized primer sets and probe sequences, spanning different exons where possible. As with all sequences analyzed using SciTools, a chosen selection of oligos can be quickly and simply transferred to an order list ready for purchase ...

Supplementary Materialsgkaa270_Supplemental_Document. libraries in which ideally each alternate codon is usually represented in equivalent measure, so that none of the potentially beneficial mutations launched in the wise library design are missed during screening. A common method for creating combinatorial libraries is to use oligonucleotides that expose codons synthesised as mixed bases (e.g. NNK) (6C8). Such oligonucleotides are relatively inexpensive and multiple mixed-based codons can be combined on a single oligonucleotide however the quality of DNA libraries is normally compromised because they present degeneracy and encode unequal proportions of proteins (9). The degeneracy issue continues ALK6 to be attended to through the introduction of small-intelligent libraries partly, using a mixture of different mixed-base codon-containing oligonucleotides (e.g. 22c-technique), although such strategies cannot deliver custom made codon ratios as well as the concentrating on of multiple sites in ...

The capping protocol will be verified by synthesizing a fluorescent cap consisting of the oligonucleotide sequence complementary to the functionalization sites as well as a fluorescent tag with an excitation emission spectra significantly different than that used in the agarose gel electrophoresis dye. Two samples, the original literature cage and the modified cage with functionalization sites, will be mixed with the fluorescent cap and incubated. These samples will be run in an agarose gel without dye, imaged, and then soaked in dye and re-imaged. The presence, and lack thereof, of fluorescence in each well will definitively confirm the capping method. Next, the peptide-polymer-cap will be synthesized using the complementary oligonucleotide, a PEG spacing molecule, and the functional peptide. The synthesis will be verified via NMR. ...

Phosphoimidazolide-activated ribomononucleotides (*pN; see Scheme I) are useful substrates for the nonenzymatic synthesis of oligonucleotides. In the presence of metal ions dilute neutral aqueous solutions of *pN (0.01 M) typically yield only small amounts of dimers and traces of oligomers; most of *pN hydrolyzes to yield nucleoside 5′-monophosphate (5′NMP). An earlier investigation of *pN reactions in highly concentrated aqueous solutions (up to 1.4 M) showed, as expected, that the percentage yield of the condensation products increases and the yield of the hydrolysis product correspondingly decreases with *pN concentration (Kanavarioti 1997). Here we report product distributions in reactions with one, two, or three reactive components at the same total nucleotide concentration. *pN used as substrates were the nucleoside 5′-phosphate 2-methylimidazolides, 2-MeImpN, with N= cytidine (C), uridine (U), or guanosine (G). Reactions were conducted as self-condensations, i.e., one nucleotide only, with

This unit describes the chemical synthesis of α‐L‐threofuranosyl nucleic acid (TNA) triphosphates for thymidine (T), guanosine (G), cytidine (C), and the diaminopurine (D) analog of adenosine and their incorporation into TNA oligonucleotides by enzyme‐mediated polymerization of a DNA primer‐template complex

Assessing incomplete deprotection of microarray oligonucleotides in situ.s profile, publications, research topics, and co-authors

The Report Antisense Oligonucleotides Market: Global Industry Analysis 2013-2017 and Opportunity Assessment 2018-2028 provides information on pricing, market analysis, shares, forecast, and company profiles for key industry participants. - MarketResearchReports.biz

Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, has further expanded its portfolio of PrimeTime® qPCR products with the launch of two new probes. The PrimeTime Eco Probe and PrimeTime Mini LNA Probe complete the existing offering, providing new options for customers performing gene expression and genotyping experiments.. Developed to support customers looking for a cost-effective option at the mid-scale range, the Eco Probe is delivered at a normalized yield of 2.5 nmoles, sufficient for approximately 500 reactions, bridging the space between the existing PrimeTime Mini qPCR Probe (0.5 nmole normalized yield, 100 reactions) and the 100 nmole synthesis scale (minimum guaranteed yield of 10 nmoles). If ordered with the option of an internal ZEN™ quencher, the double-quenched PrimeTime Eco Probe will generate less background noise, while increasing end point signal, to significantly boost qPCR sensitivity and precision when compared to traditional ...

Literature: - Mediator Probe PCR: Detection of Real-Time PCR by Label-Free Probes and a Universal Fluorogenic Reporter. Wadle S, Rubenwolf S, Lehnert M, Faltin B, Weidmann M, Hufert F, Zengerle R, von Stetten F; Methods Mol Biol. (2014), 1160:55-73. doi: 10.1007/978-1-4939-0733-5_6.. - Fluorescence signal-to-noise optimisation for real-time PCR using universal reporter oligonucleotides. Lehnert M, Kipf E, Schlenker F, Borst N, Zengerle R, von Stetten F; Anal. Methods (2018), DOI: 10.1039/c8ay00812d.. - Mediator Probe PCR: A Novel Approach for Detection of Real-Time PCR Based on Label-Free Primary Probes and Standardized Secondary Universal Fluorogenic Reporters. Faltin B, Wadle S, Roth G, Zengerle R, von Stetten F; Clinical Chemistry (2012), 58:11; 1546-1556.. - Real-time PCR probe optimization using design of experiments approach. Wadle S, Lehnert M, Rubenwolf S, Zengerle R, von Stetten F; Biomolecular Detection and Quantification 7 (2016), 1-8.. - Simplified development of multiplex real-time ...

Literature: - Mediator Probe PCR: Detection of Real-Time PCR by Label-Free Probes and a Universal Fluorogenic Reporter. Wadle S, Rubenwolf S, Lehnert M, Faltin B, Weidmann M, Hufert F, Zengerle R, von Stetten F; Methods Mol Biol. (2014), 1160:55-73. doi: 10.1007/978-1-4939-0733-5_6.. - Fluorescence signal-to-noise optimisation for real-time PCR using universal reporter oligonucleotides. Lehnert M, Kipf E, Schlenker F, Borst N, Zengerle R, von Stetten F; Anal. Methods (2018), DOI: 10.1039/c8ay00812d.. - Mediator Probe PCR: A Novel Approach for Detection of Real-Time PCR Based on Label-Free Primary Probes and Standardized Secondary Universal Fluorogenic Reporters. Faltin B, Wadle S, Roth G, Zengerle R, von Stetten F; Clinical Chemistry (2012), 58:11; 1546-1556.. - Real-time PCR probe optimization using design of experiments approach. Wadle S, Lehnert M, Rubenwolf S, Zengerle R, von Stetten F; Biomolecular Detection and Quantification 7 (2016), 1-8.. - Simplified development of multiplex real-time ...

The scale you order determines the amount of sequence-initiating base used to begin synthesis of your oligonucleotide. Since oligonucleotide synthesis (or any chemical reaction) is never 100% efficient, the amount of final product recovered after synthesis and purification steps (yield) will always be less than the starting amount used for synthesis. Modifications, secondary structures, and purification services further affect the final yield for a given construct. IDT offers guaranteed yields based on all of these factors that define the minimum amount of oligonucleotide you will receive for a given construct.. The minimum yield guarantee is listed in the shopping cart and in your confirmation email. To receive a higher final yield, select a larger synthesis scale. For further discussion on this topic, see the Technical Report, Oligonculdotide Yield, Resuspension, and Storage.. ...

Figure 1: Study of Cellular Uptake of Modified Oligonucleotides by Using Time-Resolved Microspectrofluorimetry and Florescence Imaging

Read user reviews, compare products and contact manufacturers of Oligonucleotides products, including nucleic acid detection system, kits and reagents on SelectScience.

Read user reviews, compare products and contact manufacturers of Oligonucleotides products, including nucleic acid detection system, kits and reagents on SelectScience.

Ready to Go ™ Primers are common oligonucleotides available for immediate shipment for various applications including sample preparation, sequencing, and gene expression analysis.. ...

DNA-PAINT works through the transient binding of a short imaging oligonucleotide containing a fluorophore to a complementary oligonucleotide called the docking strand on the target of interest such as an antibody, nanobody, aptamer or suicide enzyme ligand. The sample is labeled with the docking strand through conventional techniques and prepared for imaging. For imaging, the sample is bathed in imaging buffer (typically PBS but can include oxygen scavengers) and a low (typically 0.1-1 nM) concentration of imaging oligo complementary to the docking strand. The imaging oligo is typically 9-10 nucleotides in length and contains a fluorophore. We recommend Cy3B for DNA-PAINT due to its fluorogenicity and thus lower background. Once in imaging buffer the sample can be imaged. The transient binding of the imaging strand to the docking strand stops the diffusion of the fluorophore allowing it to be imaged on the camera. Since the sample is bathed in a large excess of constantly exchanging ...

DNA synthesis is the largest segment within enabling products segment, whereas oligonucleotide synthesis is expected to be fastest growing market at 57.8% CAGR during 2014 and 2020. Chassis organism would be the fastest growing core product during the forecast period with synthetic DNA occupying largest market share.

Abstract Human β-globin disorders are relatively common genetic diseases cause by mutations in the β-globin gene. Increasing the expression of the γ-globin gene has great benefits in reducing complications associated with these diseases. The Oct-1 transcription factor is involved in the transcriptional regulation of the γ-globin gene. The human γ-globin genes (both Aγ and Gγ-globin genes) carry three Oct-1 transcription factor consensus sequences within their promoter regions. We have studied the possibility of inducing γ-globin gene expression using decoy oligonucleotides that target the Oct-1 transcription factor consensus sequence. A double-stranded 22 bp decoy oligonucleotide containing the Oct-1 consensus sequence was synthesized. The results obtained from our in vitro binding assay revealed a strong competitive binding of the decoy oligonucleotide for the Oct-1 transcription factor. When K562 human erythroleukemia cells were treated with the Oct-1 decoy oligonucleotide, significant

EMEA Oligonucleotide DNA Microarrays (oDNA) market analysis of an industry is a crucial thing for various stakeholders like investors, CEOs, traders, suppliers and others. The Oligonucleotide DNA Microarrays (oDNA) industry research report is a resource, which provides current as well as upcoming technical and financial details of the industry.. Oligonucleotide DNA Microarrays (oDNA) market 2017-2022 research report is a professional and in-depth study on the current state of this market. Various definitions and classification of the industry, applications of the industry and chain structure are given. Present day status of the Oligonucleotide DNA Microarrays (oDNA) industry policies and news are analysed.. Following are major Table of Content of Oligonucleotide DNA Microarrays (oDNA) Industry:. Oligonucleotide DNA Microarrays (oDNA) Market Competition by Manufacturers, Oligonucleotide DNA Microarrays (oDNA) Production, Revenue by Region, Oligonucleotide DNA Microarrays (oDNA) Supply, ...

TY - JOUR. T1 - Oligonucleotide-based systems. T2 - DNA, microRNAs, DNA/RNA aptamers. AU - Jolly, Pawan. AU - Estrela, Pedro. AU - Ladomery, Michael. N1 - Special volume Biosensor technologies for detection of biomolecules (Ed: P. Estrela) PY - 2016/6/30. Y1 - 2016/6/30. N2 - There is an increasing number of applications that have been developed for oligonucleotide-based biosensing systems in genetics and biomedicine. Oligonucleotide-based biosensors are those where the probe to capture the analyte is a strand of DNA, RNA or a synthetic analogue to naturally occurring nucleic acids. This chapter will draw light upon various types of nucleic acids such as DNA, RNA (particularly microRNAs), their role and their application in biosensing. Also, it will cover DNA/RNA aptamers, which can be used as bioreceptors to a wide range of targets such as proteins, small molecules, bacteria and even cells. It will also highlight how the invention of synthetic oligonucleotides like PNA or LNA has pushed the ...

Introduction] Cooperative binding by proteins to DNA results in higher sequence specificity as well as greater sensitivity to concentration changes. We recently reported cooperative binding of two oligonucleotides at abutting sites by triple helix formation on double helical DNA. However, the enhanced binding observed was modest (a factor of 3.5) and likely due to favorable basestacking interactions between adjacent oligonucleotides and/or induced conformational changes propagated to adjacent binding sites. Thus, the issue arises whether cooperativity in oligonucleotide-directed triple helix formation can be enhanced by the addition of discrete dimerization domains. We report here the binding properties of oligonucleotides that dimerize by Watson-Crick hydrogen bonds and bind neighboring sites on double helical DNA by triple helix formation. ...

Antagomirs also known as anti-miRs or blockmirs are a class of chemically engineered oligonucleotides that prevent other molecules from binding to a desired site on an mRNA molecule. Antagomirs are used to silence endogenous microRNA (miR). An antagomir is a small synthetic RNA that is perfectly complementary to the specific miRNA target with either mispairing at the cleavage site of Ago2 or some sort of base modification to inhibit Ago2 cleavage. Usually, antagomirs have some sort of modification, such as 2-methoxy groups and phosphorothioates, to make them more resistant to degradation. Antagomirs are microRNA inhibitors that inhibit miRNAs but, because of the promiscuity of microRNAs, antagomirs could affect the regulation of many different mRNA molecules. It is unclear how antagomirization (the process by which an antagomir inhibits miRNA activity) operates, but it is believed to inhibit by irreversibly binding the miRNA. Blockmirs are designed to have a sequence that is complementary to an ...

An improved delivery system for antisense oligonucleotides involves a liposomal composition, comprising a liposome which consists essentially of neutral phospholipids and an antisense oligonucleotide that is entrapped in the liposome and is selected from the group consisting of phosphodiester oligonucleotides, phosphorothioate oligonucleotides, and p-ethoxy oligonucleotides.

294547663 - EP 0985148 A1 2000-03-15 - NUCLEIC ACID DIAGNOSTICS BASED ON MASS SPECTROMETRY OR MASS SEPARATION AND BASE SPECIFIC CLEAVAGE - [origin: WO9854571A1] A method of detecting a mutation or a difference of one or more nucleotides between a nucleic acid molecule to be tested and a reference nucleic acid molecule, said method comprising subjecting the test nucleic acid molecule to base specific cleavage to generate oligonucleotide fragments, separating the resulting oligonucleotide fragments based on mass by MALDI-ATOF MS and/or other equivalent procedure to produce a fingerprint of then oligonucleotide fragments comprising one or more peaks wherein a peak represents the mass of each fragment and identifying an altered peak relative to a reference nucleic acid molecule subjected to the same procedure wherein the presence of an altered peak is indicative of a difference of one or more nucleotides in said tested nucleic acid molecule.[origin: WO9854571A1] A method of detecting a mutation or a

Twisted intercalating nucleic acid (TINA) is a nucleic acid molecule that, when added to triplex-forming oligonucleotides (TFOs), stabilize Hoogsteen triplex DNA formation from double-stranded DNA (dsDNA) and TFOs. Its ability to twist around a triple bond increases ease of intercalation within double stranded DNA in order to form triplex DNA. Certain configurations have been shown to stabilize Watson-Crick antiparallel duplex DNA. TINA-DNA primers have been shown to increase the specificity of binding in PCR. The use of TINA insertions in G-quadruplexes has also been shown to enhance anti-HIV-1 activity. TINA stabilized PT demonstrates improved sensitivity and specificity of DNA based clinical diagnostic assays. Triple helixes are formed when a single-stranded triplex-forming oligonucleotide (TFO) binds to a purine-containing strand of dsDNA through specific major groove interactions. Generally, the third-strand affinity of a TFO is low, due to the requirement for the formation of pH-sensitive ...

Mutations that cause activation of the KRAS oncogene are common in human cancer, including treatment-resistant tumor types such as lung and pancreatic cancer. KRAS has also proven to be notoriously difficult to target with small molecules. To overcome this issue, Ross et al. have turned to genetic technology, demonstrating an antisense oligonucleotide-based therapy for inhibiting KRAS. The antisense oligonucleotide used in this study was chemically modified, allowing systemic delivery through subcutaneous injection and avoiding the need for a specialized delivery vehicle. The authors tested the efficacy of this therapy in multiple mouse models of non-small cell lung cancer and evaluated its safety in primates, demonstrating its potential suitability for translation to humans. ...

These locked nucleic acid (LNA) DNA and RNA oligonucleotides have increased Tm that can be modulated to provide greater sensitivity, SNP detection, and mismatch discrimination.

These locked nucleic acid (LNA) DNA and RNA oligonucleotides have increased Tm that can be modulated to provide greater sensitivity, SNP detection, and mismatch discrimination.

These locked nucleic acid (LNA) DNA and RNA oligonucleotides have increased Tm that can be modulated to provide greater sensitivity, SNP detection, and mismatch discrimination.

One oligonucleotide-based approach that appear very promising for the treatment of different genetic disorders are based on so-called splice-correcting oligonucleotides (SCOs) that are exploited to manipulate splicing patterns. In order to increase the bioavailability, cell-penetrating peptides (CPPs) have readily been covalently conjugated to SCOs to facilitate cellular internalization. While being a successful strategy for the delivery of uncharged oligonucleotides (ONs), it is extremely difficult to generate covalent conjugates between commonly used negatively charged ON analogs and cationic CPPs. Furthermore, high concentrations of ONs in the micromolar range are often needed to obtain biological responses, most likely as a result of endosomal entrapment of material. Therefore, exploring other vectorization methods using CPPs with endosomolytic properties are highly desired.A method of using stearyl modified CPP (i.e., TP10) analogs, named PepFect3 and PepFect4, are being described for the

1. de Muer, Brian and Michael Steyaert. "CMOS Fractional-N Synthesizers, KAP, 2003. 2. Hegazi, E. and A.A. Abidi. "A 17 mW Transmitter and Frequency Synthesizer for 900-MHz GSM Fully Integrated in 0.35-ݏ CMOS, IEEE Journal of Solid State Circuits, Vol 38, n5, May 2003. 4. McMahill, D.R. and C.G. Sodini. "A 2.5-Mb/s GFSK 5.0-Mb/s 4-FSK Automatically Calibrated Sigma-delta Frequency Synthesizer, IEEE Journal of Solid State Circuits, Vol 37, n1 January 2002. 3. Leenaerts, Domine, Johan van der Tang and Cicero Vaucher. "Circuit Design for RF Transceivers, Kluwer AP, 2001. 5. Rategh, Hamid R. and Thomas H. Lee. "Multi-GHz Frequency Synthesis & Division Frequency Synthesizer Design for 5 GHz Wireless LAN Systems, Kluwer AP, 2001.. 6. Rhee, W., B.S. Song and A. Ali. "A 1.1 GHz CMOS Fractional-N Frequency Synthesizer with a 3-b Third-order Delta-sigma Modulator, IEEE Journal of Solid State Circuits, Vol 35, n10, Oct 2000. 7. Riley, Thomas A.D., et al. "Techniques for ...

Thermo Scientific™ M13/pUC sequencing primer (-20), 17-mer 10uM, 6nmol Unlabeled Oligonucleotides and Primers Oligonucleotides

Your partner for superior pre-clinical custom oligonucleotide manufacturing services. OliGrow® is a business unit of Avecia with a focus on small scale oligonucleotide manufacturing for pre-clinical use.. Combining phase appropriate quality and purity methods with the high level of customer service and expertise that Avecia is known for, OliGrow® delivers pre-clinical oligonucleotides at μmol- to mmol-scales, with affordable pricing and fast turn-around times. OliGrow® is able to offer custom DNA and RNA with standard chemical modifications along with solutions for more specialized needs, including specialty amidites and oligonucleotide conjugates.. ...

Looking for oligonucleotide? Find out information about oligonucleotide. A deoxyribonucleic acid or ribonucleic acid sequence composed of two or more covalently linked nucleotides. Oligonucleotides are classified as... Explanation of oligonucleotide

Oligonucleotide probes are effective tools for the research of nucleic acids. This project discusses about Oligonucleotide Probes, Multiplex Genetic Analyses, Multiplex nucleic acid analyses, Genetic variation, Different Types of nucleic acid analyses, multiplex oligonucleotide design,

Supplementary MaterialsDocument S1. Pairs of mice are denoted 1, 2, and 3. mmc3.xlsx (27K) GUID:?E634A462-7359-492B-9AB4-6D88E6AB25BE Table S3. qRT-PCR Primers and siRNA Oligonucleotides, Linked to the Superstar Methods Supply and nucleic acidity series of DNA oligonucleotides found in qRT-PCR analyses, and the L-cysteine foundation and concentrating on sequences of double-stranded RNA oligonucleotides found in RNAi tests. mmc4.xlsx (17K) GUID:?4E6F67A3-206F-4D89-884E-B620530F1FD3 Movie S1. Live Imaging of CCPG1 Foci over the ER, Linked to Amount?4 HeLa GFP-CCPG1 cells had been packed with ER tracker crimson dye, starved in EBSS, and imaged for both fluorophores by time-lapse rotating drive confocal microscopy. z stacked pictures made up of three specific sections were attained every 20 s. Three representative movies sequentially are proven. Start times of Rabbit polyclonal to WAS.The Wiskott-Aldrich syndrome (WAS) is a disorder that results from a monogenic defect that hasbeen mapped to the ...

There are a few things that stand out immediately in the above table. Read/write speeds are very slow for MoSS, and the cost per gigabyte is 500 million times more than just using a magnetic tape. While the cost of sequencing the genome has fallen off a cliff over the past decade, its still prohibitively expensive at this point in time.. Helixworks isnt the only company working on using DNA for data storage. Companies like Microsoft have been exploring this realm as well. Microsoft started by taking 200 MB worth of data containing documents, artwork, and even a music video and converting all to 1s and 0s. They then sent the sequence to Twist Bioscience to encode it in a DNA strand. Twist sent back the strand to Microsoft and they actually read the data. Microsoft is confident enough that DNA data storage will work that they agreed to purchase ten million long oligonucleotides from Twist Bioscience to encode digital data. They are working with researchers at the University of Washington on the ...

Good methods must be reliable, well-tested, and honed to minimize the time and expense required to achieve the desired results. CPNC provides a continuously growing and evolving set of protocols that allows researchers to benefit from the experience of other researchers around the world. The core manual provides a comprehensive set of protocols that have been compiled, revised, and streamlined over the last 6 years. Quarterly updates provide new protocols in emerging areas of research as well as continued advances and new applications for fundamental methods. The book is designed to grow and change with the field of nucleic acid chemistry. Fundamental nucleoside chemistry methods include sugar-base condensation, phosphorylation, and nucleoside protection. Methods for oligonucleotide synthesis include H-phosphonate and phosphoramidite approaches, solid-phase and solution-phase synthesis, large-scale synthesis, synthesis for modified and unmodified oligonucleotides, conjugation of ...

LANGUAGE NoImplicitPrelude #-} {- , Noise and random processes. This uses a fast reimplementation of System.Random.randomR since the standard function seems not to be inlined (at least in GHC-6.8.2). -} module Synthesizer.State.NoiseCustom where import qualified Synthesizer.State.Signal as Sig import qualified Algebra.RealField as RealField import qualified Algebra.Field as Field import qualified Synthesizer.RandomKnuth as Knuth import System.Random (Random, RandomGen, ) import qualified System.Random as Rnd import qualified Prelude as P import NumericPrelude.Base import NumericPrelude.Numeric {-, Deterministic white noise, uniformly distributed between -1 and 1. That is, variance is 1\/3. -} {-# INLINE white #-} white :: (Field.C y, Random y) =, Sig.T y white = whiteGen (Knuth.cons 12354) {-# INLINE whiteGen #-} whiteGen :: (Field.C y, Random y, RandomGen g) =, g -, Sig.T y whiteGen = randomRs (-1,1) {- , Approximates normal distribution with variance 1 by a quadratic B-spline distribution. ...

Within the last few decades, advancements in methods of DNA synthesis, as well as synthesizing, DNA sequences have improved our capabilities of understanding genomics which has led to improved capability of engineering biological systems. The chemically synthesized DNA oligonucleotides and their assembly constructs contain encoded genes, synthons, circuits, metabolic pathways and even whole genomes. These assemblies have the ability to design, build, and test various research topics within molecular technology and synthetic biology. At Synbio Technologies we pride ourselves in our ability to successfully synthesize the requested DNA sequence specific to each customer. We have the ability, through our Syno Gene Synthesis Platform, to synthesize sequences up to and including 200kb in length with guaranteed one hundred percent accuracy.. DNA synthesis from oligonucleotides. As a polymer, consisting of four different phosphoramidite monomers, DNA synthesis follows a form of hierarchical polymer ...

Antisense oligonucleotides have been investigated as potential drugs for years. They inhibit target gene or protein expression. The present review summarizes their modifications, modes of action, and applications of liquid chromatography coupled with mass spectrometry for qualitative and quantitative analysis of these compounds. The most recent reports on a given topic were given prominence, while some early studies were reviewed in order to provide a theoretical background. The present review c

Considering the crucial role of miRNAs in vascular remodeling, therapeutic strategies to either increase or inhibit miRNAs might be a promising approach. When compared with other molecular targets, the targeting of miRNAs has several theoretical advantages: 1) the antisense oligonucleotides to inhibit endogenous miRNAs are short, which makes packaging and delivery easier; 2) cell-specific expression of miRNAs and suppression of disease-specific targets may attenuate off-target effects of the treatment; and 3) because of regulatory effects of miRNAs, therapeutic modulation of miRNA levels may allow more rapid and subtle interference with molecular disease mechanisms.. Several methods for miRNA loss-of-function in vivo studies using modified antisense oligonucleotides have been developed. Intravenous administration of antagomirs, a class of nucleotides chemically modified with 2′-O-methyl phosphoramidites, and a hydroxyprolinol-linked cholesterol solid support, suppresses the expression of ...

Professional bodies and Institutes CPD schemes are either structured as Input or Output based. Input based schemes list a precise number of CPD hours that individuals must achieve within a given time period. These schemes can also use different currencies such as points, merits, units or credits, where an individual must accumulate the number required. These currencies are usually based on time i.e. 1 CPD point = 1 hour of learning. Output based schemes are learner centred. They require individuals to set learning goals that align to professional competencies, or personal development objectives. These schemes also list different ways to achieve the learning goals e.g. training courses, seminars or e:learning, which enables an individual to complete their CPD through their preferred mode of learning. The majority of Input and Output based schemes actively encourage individuals to seek appropriate CPD activities independently. As a formal provider of CPD certified activities, SMI Group ...

Bio-Synthesis is a provider of Custom Oligo Synthesis services. Oligonucleotides are synthesized as unmodified or modified chemical structures with defined sequences using a procedure known as Phosphoramidite solid-phase synthesis. Oligos which are

/PRNewswire/ -- GenScript, the worlds largest provider of synthetic genes, has launched a new GenPlus™ Next-Generation Gene Synthesis service, which offers...

PrimeTime LNA Probes are 5 nuclease probes which incorporate nucleic acid monomers into the probe sequence to increase hybridization temperature.

OriGene and Blue Heron, Gold Standard of Gene Synthesis, working together to build any DNA construct you design, from single gene synthesis to whole genome synthesis

Artificial DNA: tools and functions introduces the idea that of man-made DNA that has been rationally designed and explains the way it might be exploited to be able to enhance items that may in achieving your meant goal. the 1st a part of the booklet covers tools of oligonucleotide synthesis and direct purposes of man-made DNA. the second one half describes equipment of gene meeting from man made oligonucleotides and functions of man-made genes. The authors additionally speak about different traits and destiny advancements inside every one program zone ...

Lathe, R.F., Lecocq, J.P. and Everett, R.D. (1983) DNA engineering: the use of enzymes, chemicals and oligonucleotides to re-structure DNA in vitro. In: Williamson, R. (ed.) Genetic Engineering. Academic Press: London, pp. 1-56. ISBN 9780122703041 Full text not currently available from Enlighten. ...

The miRIDIAN microRNA Hairpin Inhibitors are single-stranded chemically-modified RNA oligonucleotides designed to inhibit the function of endogenous miRNA. miRIDIAN microRNA Hairpin Inhibitors specifically target each mature microRNA as derived from a unique precursor and exhibit greater potency and longevity than 2 -O- methyl RNA oligos that are antisense to the mature microRNA. For additional information please read the miRIDIAN brochure and flier ...

Oligonucleotides can be synthesized by condensing a protected nucleoside H‐phosphonate monoester with a second nucleoside in the presence of a coupling agent to produce a dinucleoside H‐phosphonate diester

Biotage are pleased to announce the launch of new heating blocks and heating plates accessories for the Syro family of peptide synthesizers, which enable the heating of multiple peptides in parallel. This heating capability, combined with the strengths of the Syro peptide synthesizers, will continue to make them the perfect choice for peptide synthesis applications that demand a high through put.. To learn more, please follow the link for more information on the new Syro heating blocks and heating plates accessories.. ...

Protein Technologies Sonata Peptide Synthesizer With Computer.The Sonata mid-scale peptide synthesizer is specifically designed for automated solid phase…

NEW YORK, March 28, 2017 /PRNewswire/ -- The telecommunications application is the largest contributor to the frequency synthesizer market The overall frequency synthesizer market is expected ...

Oligonucleotides for therapeutic purposes gained increased importance during the past 20 years. This was furthered by advances in biomedical research, improving drug properties like degradation stability, target binding and pharmacokinetics. Different classes of oligonucleotide therapeutics exist, referring to their mode of interaction with the target molecules. Oligonucleotides binding to mRNA rely on a Watson-Crick base-paring mechanism. The goal is to identify sequences that are highly specific for the target RNA, but do not unintendedly bind homologous RNA. However, also aspects such as tertiary structures and molecular interactions established by RNA under physiological conditions as well as aspects of drug safety and delivery have to be taken into account. Two principle modes of action can be discriminated, a target RNA degrading and a target RNA non-degrading. Mechanisms, which decrease the expression levels of specific proteins are referred to as gene silencing. In their degrading ...

Our facility provides services for DNA/RNA sequencing and oligonucleotide synthesis. Our sequencing technology portfolio includes first-generation sequencing (classic Sanger and fragment analysis), second-generation sequencing (next-generation sequencing) using Illumina instruments, and (…). ...

The accuracy of design and synthesis of a primer pair is the most important consideration to generate good PCR performance data. Poor design choices, erroneous or truncated sequences, and ineffective purification can lead to unusable results. We have applied thermodynamic and bioinformatic knowledge towards a suite of easy-to-use, online tools to help you design primers. We also enable consistent, quality data by providing primers in a variety of formats that have been synthesized on our state-of-the-art oligonucleotide synthesis platforms.

Oligonucleotide quantification methods and units can vary between manufacturers. Be sure you accurately measure and dilute your DNA and RNA oligos.

Antigene Bacteria 3D Model available on Turbo Squid, the worlds leading provider of digital 3D models for visualization, films, television, and games.

A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.

Regulatory and validation aspects of oligonucleotide analysis Ipsita Roymoulik, Ph.D.(Avecia Biotechnology), will describe methodology for robust method transfer into manufacturing. Yansheng Wu, Ph.D. (Archemix), will discuss analysis of oligonucleotide aptamers and PEGylated oligos, both with novel applications compared with RNAi. Martin Gilar, Ph.D. (Waters), will present methods for analyzing single- and double-stranded RNAi therapeutics. A live Q&A session will follow the presentations, offering you a chance to pose questions to our expert panelists.. Register today at www.genengnews.com/oligos. ...

EbolaID (http://ebolaid.portugene.com) provides a complete, quality checked and regularly updated list of oligonucleotides for the Ebola virus. The database describes the genetic diversity across the Ebola genome to facilitate the design of accurate diagnostic methods and therapeutic approaches. The database provides an interface for browsing, filtering, and downloading data from published oligonucleotide sequences (PCR primers, real-time PCR probes, etc.) annotated according to a reference genome. The user can find varied information for each oligonucleotide: the type of technique where it was originally used, the sequence, location in the reference genome, references, etc. The database provides different measures of sequence conservation for each target region obtained from multiple sequence alignments, allowing the selection of the most conserved oligonucleotides.. ...