The invention consists of oligonucleotides which inhibit the immunostimulatory activity of ISS-ODN (immunostimulatory sequence oligodeoxynucleotides) as well as methods for their identification and use. The oligonucleotides of the invention are useful in controlling therapeutically intended ISS-ODN adjuvant activity as well as undesired ISS-ODN activity exerted by recombinant expression vectors, such as those used for gene therapy and gene immunization. The oligonucleotides of the invention also have anti-inflammatory activity useful in reducing inflammation in response to infection of a host with ISS-ODN containing microbes, in controlling autoimmune disease and in boosting host Th2 type immune responses to an antigen. The invention also encompasses pharmaecutically useful conjugates of the oligonucleotides of the invention (including conjugate partners such as antigens and antibodies).
Page contains details about 3-tocopherol-modified 1826 CpG oligonucleotide micelle-SIINFEKL conjugates . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Oligodeoxyribonucleotides containing CpG motifs (CpG ODNs) are widely used for activation of immune cells, such as human PBMCs, murine splenocytes or isolated immune cells, e.g., B cells and pDCs. They also activate signalling in TLR9-expressing recombinant cell lines. ODN 1826 strongly activates murine TLR9. It is a B-class ODN for activation of B cells, induction of Il-6 secretion, and activation of NF-kB-signalling pathways. - USA
Oligodeoxyribonucleotides containing CpG motifs (CpG ODNs) are widely used for activation of immune cells, such as human PBMCs, murine splenocytes or isolated immune cells, e.g., B cells and pDCs. They also activate signalling in TLR9-expressing recombinant cell lines. ODN 1826 strongly activates murine TLR9. It is a B-class ODN for activation of B cells, induction of Il-6 secretion, and activation of NF-kB-signalling pathways. - Belgique
CpG oligodeoxynucleotide treatment enhances innate resistance and acquired immunity to African trypanosomes.by Harris TH, Mansfield JM, Paulnock DM. MiniManuscript.
Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5-phosphate and 3-OH groups.. The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions.. ...
The invention relates to novel modified oligonucleotides, the construction thereof, and their use in oligonucleotide-based therapies. More specifically, the invention is to novel oligonucleotides having modified internucleoside linkages which are resistant to nucleases, having enhanced ability to penetrate cells, and which are capable of binding target oligonucleotide sequences in vitro and in vivo. The modified oligonucleotides of the invention are particularly useful in oligonucleotide-based therapies utilizing the modified oligonucleotides to interrupt protein synthesis or transcription or to otherwise inactivate messenger RNA or double stranded DNA.
Abstract. CpG ODN are being actively investigated as cancer vaccine adjuvants because they mature plasmacytoid dendritic cells (pDC) into potent antigen-presen
DNA synthesis: Oligodeoxynucleotides were synthesized on a Cyclone Plus DNA Synthesizer (Millipore, Marlborough, MA) using standard phosphoramidite chemistry. Precursor phosphoramidites were purchased from PerSeptive Biosystems (Farmington, MA) or from Glenn Research (Sterling, VA). They were then purified using Oligo-Pure cartridges (Hamilton, Reno, NV) according to the manufacturers protocol. This was followed by 32P end-labelling, as previously described (Smith, et al. 1991). Mobility Shift Assays: Duplexes were formed by combining equimolar (20 µM) amounts of complementary strands in annealing buffer (10 mM Tris-HCl pH 7.4, 1 mM EDTA, and 100 mM NaCl) then treating with 95°C for 5 minutes and 50°C for 60 minutes. The samples were allowed to cool to room temperature for 10 min. and were then stored on ice until needed. Methyltransferase Purification: M·HhaI was obtained via purification from E. coli RR1 containing the pSP72 plasmid (Promega, Madison, WI) carrying the entire HhaI ...
117D: Crystal and molecular structure of the alternating dodecamer d(GCGTACGTACGC) in the A-DNA form: comparison with the isomorphous non-alternating dodecamer d(CCGTACGTACGG).
After testing the ODNs, an effective ODN or ODNs are chosen; a rough guideline for an effective ODN is one which depletes the target mRNA to less than 20% of the level of the uninjected at a 10 ng dose, as seen by northern analysis. The ODNs so selected are generally resynthesized in a modified form. The modification suggested by experiments of Baker, et al. (1990) and Dagle, et al. (1990) is used, where 3-4 of the 5-most and 3-most phosphodiester bonds are replaced by phosphorothioate bonds, leaving at least 8 unmodified bonds in the center of the ODN (e.g. Kofron et al., 1997). Modified oligodeoxynucleotides provide two major advantages over unmodified ODNs. The modified ODNs can deplete the message more effectively than the unmodified ODNs at a much lower concentration (e.g. compare the 2 ng depletion by modified ODN #7 in fig. 4b to the 10 ng unmodified ODN #7 depletion in fig. 4a). Second, the modified ODNs provide a more reliable and reproducible depletion. However, it has been our ...
TY - JOUR. T1 - CpG oligonucleotides enhance the tumor antigen-specific immune response of a granulocyte macrophage colony-stimulating factor-based vaccine strategy in neuroblastoma. AU - Sandler, Anthony D.. AU - Chihara, Hiroshi. AU - Kobayashi, Gen. AU - Zhu, Xiaoyan. AU - Miller, Michal A.. AU - Scott, David. AU - Krieg, Arthur M.. PY - 2003/1/15. Y1 - 2003/1/15. N2 - Granulocyte macrophage colony-stimulating factor (GM-CSF)-transduced autologous tumor cells form the basis of many immunotherapeutic strategies. We tested whether combining this approach with T-helper 1 (Th-1)-like immunostimulatory CpG oligodeoxynucleotides (CpG ODNs) would improve therapeutic efficacy in an established model of murine neuroblastoma. The weakly immunogenic Neuro-2a cell line was used in syngeneic A/J mice. CpG 1826 was tested for its antitumor effect alone and as an adjuvant to Neuro-2a cells retrovirally transduced to express murine GM-CSF (GM/Neuro-2a). Three days after wild-type (WT) tumor cell inoculation, ...
92118DNAArtificial SequenceA synthetic DNA fragment 1aaggagcgat cgccatgn 18210DNAArtificial SequenceA synthetic DNA fragment, wherein nnn is the first codon which is 3 to the start codon followed by the remainder of an open reading frame 2cgccatgnnn 10312DNAArtificial SequenceA synthetic DNA fragment 3nnnnnngtct tc 12410DNAArtificial SequenceA synthetic DNA fragment 4nnnngaagag 10513DNAArtificial SequenceA synthetic DNA fragment 5gcagcnnnnn nnn 13611DNAArtificial SequenceA synthetic DNA fragment 6nnnnngagac g 11711DNAArtificial SequenceA synthetic DNA fragment 7gccnnnnngg c 11814DNAArtificial SequenceA synthetic DNA fragment 8ggatgnnnnn nnnn 14911DNAArtificial SequenceA synthetic DNA fragment 9nnnnngagac c 111010DNAArtificial SequenceA synthetic DNA fragment 10gacgcnnnnn 101111DNAArtificial SequenceA synthetic DNA fragment 11ccnnnnnnng g 111211DNAArtificial SequenceA synthetic DNA fragment 12gcnnnnnnng c 111310DNAArtificial SequenceA synthetic DNA fragment 13nnnnngagac 101411DNAArtificial ...
Oligonucleotides are short nucleic acid polymers used in research, genetic testing and forensics. Oligonucleotides are usually made up of 13 to 25 nucleotides and are designed to hybridize specifically to DNA or RNA sequences. Solid-phase clinical synthesis is used to manufacture these small bits of nucleic acid for use in polymerase chain reaction (PCR), DNA sequencing, library construction and artificial gene synthesis.. Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure/sequence.In the enzymatic process of DNA synthesis, deoxy-ribonucleotide-5-tri-phosphates are used as substrates during polymerization. In addition, this process requires a primer and a template.. Full Research Report On Global Oligonucleotide Synthesis Market Analysis available @ https://www.millioninsights.com/industry-reports/oligonucleotide-synthesis-market. The most frequently used oligonucleotide synthesis strategy is based upon ...
Oligonucleotides are short nucleic acid polymers used in research, genetic testing and forensics. Oligonucleotides are usually made up of 13 to 25 nucleotides and are designed to hybridize specifically to DNA or RNA sequences. Solid-phase clinical synthesis is used to manufacture these small bits of nucleic acid for use in polymerase chain reaction (PCR), DNA sequencing, library construction and artificial gene synthesis.. Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure/sequence.In the enzymatic process of DNA synthesis, deoxy-ribonucleotide-5-tri-phosphates are used as substrates during polymerization. In addition, this process requires a primer and a template.. Full Research Report On Global Oligonucleotide Synthesis Market Analysis available @ https://www.millioninsights.com/industry-reports/oligonucleotide-synthesis-market. The most frequently used oligonucleotide synthesis strategy is based upon ...
In this study, we report the development of a novel, rationally designed immunostimulatory adjuvant based on chemical conjugation of CpG oligodeoxynucleotide (ODN) to the nontoxic B subunit of cholera toxin (CTB). We demonstrate that the immunostimulatory effects of CpG can be dramatically enhanced by conjugation to CTB. Thus, CpG ODN linked to CTB (CTB-CpG) was shown to be a more potent stimulator of proinflammatory cytokine and chemokine responses in murine splenocytes and human PBMCs than those of CpG ODN alone in vitro. The presence of CpG motif, but not modified phosphorothioate ODN backbone, was found to be critical for the enhanced immunostimulatory effects of CTB-CpG. Our mode-of-action studies, including studies on cells from specifically gene knockout mice suggest that similar to CpG, CTB-CpG exerts its immunostimulatory effects through a TLR9/MyD88- and NF-kappaB-dependent pathway. Surprisingly, and as opposed to CpG ODN, CTB-CpG-induced immunity was shown to be independent of ...
Disclosed is a method for detecting a nucleic acid target sequence by formation of triple helix nucleic acid structures. The method may, but need not, involve amplifying the nucleic acid in vitro using cycles of denaturation and amplification to yield product duplexes, and detecting the product duplexes by hybridizing a third strand of nucleic acid to the product duplexes without denaturation. The triple helix-forming duplex sequences may be endogenous to the target sequence being detected, or they may be introduced in the probes used during amplification.
Microbial DNA sequences containing unmethylated CpG dinucleotides activate Toll-like receptor 9 (TLR9). We have found that TLR9 is localized to the endoplasmic reticulum (ER) of dendritic cells (DCs) and macrophages. Because there is no precedent for immune receptor signaling in the ER, we investigated how TLR9 is activated. We show that CpG DNA binds directly to TLR9 in ligand-binding studies. CpG DNA moves into early endosomes and is subsequently transported to a tubular lysosomal compartment. Concurrent with the movement of CpG DNA in cells, TLR9 redistributes from the ER to CpG DNA-containing structures, which also accumulate MyD88. Our data indicate a previously unknown mechanism of cellular activation involving the recruitment of TLR9 from the ER to sites of CpG DNA uptake, where signal transduction is initiated.
Animals, Antibodies/pharmacology, Arthritis/genetics/*immunology, CpG Islands/*immunology, Freunds Adjuvant, Oligodeoxyribonucleotides/immunology/*pharmacology, Rats, Rats; Inbred Lew, Receptors; Antigen; T-Cell; alpha-beta/immunology, T-Lymphocytes/*immunology ...
Oligonucleotides are single-stranded and short RNA or DNA molecule that have a wide range of applications in research forensics and genetic testing. Oligon
Degenerate DNA Oligonucleotides. Our team offers the synthesis of DNA oligonucleotides with equimolar mixtures of two or more different bases at a given position within the sequence. Synthesis scales of 50 nmole and 200 nmole are available. No extra charges apply. -------------------------------------------------------------------------------------------------------------------------------------------. Contact us! Please let us know what your individual research needs are. We can accommodate special requests that might not be explicitly listed here. We also offer free technical assistance to help you make the best choice for your specific application.. For a brief introduction of our DNA Oligonucleotide Synthesis unit and an overview of our products and services, please see here.. ...
Biocompatible materials with nano-scale structure hold great promise for controlled and targeted delivery and half-life extension of both small-molecule drugs and various classes of biologics, such as peptides, proteins, plasmid DNA and synthetic oligodeoxynucleotides. Promising delivery systems include microcapsules, liposomes, macromolecular conjugates, nanoparticles, dendrimers, and biological stuctures such polypeptides, benign viruses and bacteriophages. This symposium will focus on commercially promising new materials and approaches. ...
Terminal lipophilization of a unique DNA dodecamer by various nucleolipid headgroups: Their incorporation into artificial lipid bilayers and hydrodynamic properties
291995831 - EP 1212085 B1 2007-10-31 - COMPOSITIONS FOR STIMULATING CYTOKINE SECRETION AND INDUCING AN IMMUNE RESPONSE - [origin: WO0115726A2] Lipid-nucleic acid particles can provide therapeutic benefits, even when the nucleic acid is not complementary to coding sequences in target cells. It has been found that lipid-nucleic acid particles, including those containing non-sequence specific oligodeoxynucleotides, can be used to stimulate cytokine secretion, thus enhancing the overall immune response of a treated mammal. Further, immune response to specific target antigens can be induced by administration of an antigenic molecule in association with lipid particles containing non-sequence specific oligodeoxynucleotides. The nucleic acid which is included in the lipid-nucleic acid particle can be a phosphodiester (i.e., an oligodeoxynucleotide consisting of nucleotide residues joined by phosphodiester linkages) or a modified nucleic acid which includes phosphorothioate or other modified linkages, and may
Fast and reliable custom oligonucleotide synthesis service at competitive price. Bioneer is one of the worlds largest suppliers of synthetic oligonucleotides. Every Bioneer oligo is purified free-of-charge utilizing our unique Bio-RP cartridge purification technology and quality checked by MALDI-TOF.
5DNB: Structure of the B-DNA decamer C-C-A-A-C-G-T-T-G-G and comparison with isomorphous decamers C-C-A-A-G-A-T-T-G-G and C-C-A-G-G-C-C-T-G-G.
TY - JOUR. T1 - Phosphorothioate-modified oligodeoxynucleotides inhibit human cytomegalovirus replication by blocking virus entry. AU - Luganini, Anna. AU - Caposio, Patrizia. AU - Landolfo, Santo. AU - Gribaudo, Giorgio. PY - 2008/3. Y1 - 2008/3. N2 - Studies in animal models have provided evidence that Toll-like receptor 9 (TLR9) agonists, such as synthetic oligodeoxynucleotides (ODNs) that contain immunostimulatory deoxycytidyl-deoxyguanosine (CpG) motifs (CpG ODNs), protect against a wide range of viral pathogens. This antiviral activity has been suggested to be indirect and secondary to CpG-induced cytokines and inflammatory responses triggered through TLR9 activation. However, few studies have addressed the potential of CpG ODNs as direct antiviral agents. Here, we report on the ability of some CpG ODNs to directly suppress, almost completely, human cytomegalovirus (HCMV) replication in both primary fibroblasts and endothelial cells. Murine CMV replication was inhibited as well, whereas no ...
Novel compounds that mimic and/or modulate the activity of wild-type nucleic acids. In general, the compounds are phosphorothioate oligonucleotides wherein the 5′-terminal internucleoside linkage or the 5′- and 3′-terminal linkages are modified.
93119DNAArtificial sequenceSingle strand DNA oligonucleotide 1ttctcccttc tccctctgc 19218DNAArtificial sequenceSingle strand DNA oligonucleotide 2gtggtcccaa gacaatgc 18320DNAArtificial sequenceSingle strand DNA oligonucleotide 3cctgcctctg ttgagactcc 20427DNAArtificial sequenceSingle strand DNA oligonucleotide 4ttctgctaac agtattcttt aatgtga 27521DNAArtificial sequenceSingle strand DNA oligonucleotide 5tgaagagttt gattccgaac g 21618DNAArtificial sequenceSingle strand DNA oligonucleotide 6agccagggta tggctgct 18720DNAArtificial sequenceSingle strand DNA oligonucleotide 7cagcctgact ggacacagaa 20820DNAArtificial sequenceSingle strand DNA oligonucleotide 8atctactgtg cccagcgact 20924DNAArtificial sequenceSingle strand DNA oligonucleotide 9gaaaggatca tctctacttt ctgg 241019DNAArtificial sequenceSingle strand DNA oligonucleotide 10gtggcgagat gctagacag 191124DNAArtificial sequenceSingle strand DNA oligonucleotide 11attttgaact tgagcaggta gttg 241220DNAArtificial sequenceSingle strand DNA oligonucleotide ...
TY - JOUR. T1 - The use of degenerate, sensor gene-specific, oligodeoxyribonucleotide primers to amplify DNA fragments from Staphylococcus aureus. AU - Bayles, Kenneth W.. PY - 1993/1/15. Y1 - 1993/1/15. N2 - The sensor proteins of bacterial two-component regulatory systems comprise a large family of proteins that are involved in environmental sensing and signal transduction. To study these proteins in the Gram+ pathogen, Staphylococcus aureus, two pairs of degenerate oligodeoxyribonucleotides (oligos) that corresponded to conserved sequences contained within sensor protein-encoding genes were synthesized. Using these oligo primers, DNA fragments from S. aureus were amplified by polymerase chain reaction (PCR), cloned in Escherichia coli, and sequenced. Comparison of the deduced amino acid sequences from these cloned fragments to the sequences contained in the GenBank database suggest that some of the PCR products were derived from sensor protein-encoding genes. However, several other fragments ...
This unit provides a modified phosphoramidite method to synthesize oligodeoxyribonucleotides onto a universal and reusable hydroxyl solid support thanks to the use of deoxyribonucleoside tert‐butyl and cyanoethyl phosphoramidites
AIM: To determine the optimum form of labelling and the most efficient reporter molecule for non-radioisotopic in situ hybridisation (ISH). METHODS: Nine deoxyoligonucleotides complementary to histone mRNA were synthesised and labelled either enzymatically or during solid-phase synthesis with the reporter molecules digoxigenin, 2,4-dinitrophenyl (DNP), or alkaline phosphatase. Pooled deoxyoligonucleotide cocktails were then used in non-radioisotopic ISH detection of histone mRNA in human tonsil. Hybrid detection was by nitroblue tetrazoleum/5-bromo-4-chloro-3-indolyl phosphate colorimetric development. RESULTS: The use of a spacer in 3 enzymatic labelling and when labelling with alkaline phosphatase significantly increased ISH signal. The 3 and 5 labelling of oligonucleotides with triple DNP groups during solid-phase synthesis produced the strongest signal as determined by the highest cell signal intensity and shortest development time. CONCLUSIONS: 3 and 5 solid-phase labelling with triple ...
Oligonucleotide synthesis market segmented global market by product(Synthesized Oligonucleotides, Custom Oligonucleotides), application(Research, Therapeutics, Diagnostics), end users & region.
Descriptive info: Homepage.. Welcome to BioSpring.. BioSpring , The Oligo Company.. Expertise in oligonucleotide synthesis.. Flexibility by open source oligonucleotide production system.. Synthesis of oligonucleotides.. Standard analyses of oligonucleotides.. Additional analyses of oligonucleotides.. Superstructures: Biological significance and structure.. siRNA, RNAi.. Interfering RNA.. Hybridisation of siRNA molecules.. Quality and delivery time.. Phosphonoacetates.. Antisense molecules.. RNase H activity.. Transport into the cells.. Advantages of phosphonoacetates.. Scales and Modifications.. Unmodified oligonucleotides.. Phosphorothioates.. RNA.. Modified RNA.. Methylphosphonates.. Phosphonoacetate oligonucleotides (PACE).. RNAi, siRNA miRNA.. 5 modifications.. 3 modifications.. Internal modifications.. Dual labeled probes.. Additional services.. Delivery.. Qualification and validation.. BioSpring GMP Manufacturing movie.. Oligonucleotides for diagnostic applications.. Certificate ISO ...
There is evidence from mouse models that CpG DNA acts as a potent vaccine adjuvant for promoting Th1-like immune responses (2-7, 10, 29-32). In contrast, very little published data about CpG DNA effects on human cells are available (10, 28, 33), and no reports whether CpG DNA may activate human DCs, which is currently an area of great therapeutic interest, and where GMCSF has shown much promise. In the present report we demonstrate that CpG DNA is a more potent stimulus than GMCSF for inducing primary blood DC survival, differentiation, activation, maturation, and the functional ability to promote a Th1-like T cell response.. CpG DNA was superior to GMCSF in preserving in vitro survival of primary blood DCs and inducing differentiation, which was reflected by an increase in cell size, granularity, and MHC II expression. CpG treatment led to activation of DCs as represented by up-regulation of the costimulatory molecules ICAM-1 (CD54), B7-2 (CD86), and CD40 and to maturation indicated by ...
The present invention relates to synergistic combinations of immunostimulatory CpG oligonucleotides and immunopotentiating cytokines. In particular, the invention relates to methods of stimulating an immune response using the synergistic combination of compounds and products related thereto.
We have proposed previously that the RIα regulatory subunit of PKA-I is an ontogenic growth-inducing protein and that its constitutive expression disrupts normal ontogenic processes, resulting in a pathogenic outgrowth such as cancer (2) . The results presented here confirm this view and suggest that the RIα antisense, which works through the Watson-Crick base pairing mechanism of action, can serve as a single gene-based therapeutic agent for cancer. The sequence-specific mechanism of action of RIα antisense is strongly supported by the experimental data that MBO antisense, having an increased hybridizing capacity and nuclease resistance, increased the antisense effect of growth inhibition, whereas the mismatched MBO control oligos could not mimic the antisense effects.. In this study, minimization of the polyanionic nature of the PS-oligo and modifications of the immunostimulatory CpG motif were two important goals for us to demonstrate the sequence-specific antisense effects in the absence ...
Coley Pharmaceutical, in collaboration with sanofi-aventis, is developing novel, immunostimulatory CpG toll-like receptor 9 (TLR9) agonists for the treatment of
Ziel dieses Arbeitsgebiets ist die präklinische Validierung von Decoy- Oligodesoxynukleotiden (ODN) als neue Wirkstoffklasse zur Prävention bzw. Therapie der Herzinsuffizienz. Decoy ODNs (decoy = Köder, Lockvogel) sind doppelsträngige, meist 15 bis 20 Basenpaare kurze synthestische DNA-Moleküle, die spezifisch die DNA-Bindungsstelle bestimmter Regulatorproteine (Transkriptionsfaktoren) im Genom nachahmen. Sie interferieren mit der zumeist aberranten Expression krankheitsrelevanter Gene, indem sie gezielt an den für die Expression dieser Gene verantwortlichen Transkriptionsfaktor binden und ihn damit hemmen. Drei verschiedene Transkriptionsfaktoren werden als potentielle therapeutische Zielmoleküle untersucht. Wichtigstes Kriterium für ihre Auswahl ist ihre nachgewiesene Beteiligung an der Expression von Genen, die für die Ausprägung der verschiedenen Varianten der terminalen Herzinsuffizienz primär verantwortlich zu sein scheinen ...
All current genetic testing methods require synthetic oligonucleotide primers and probes. At ATDBio we make high quality custom qPCR and LAMP primers and probes, working with our customers to help them develop simpler, faster and more accurate diagnostic methods. Were using our specialist nucleic acid expertise to accelerate the global response to the pandemic, helping to get the world back up and running quickly and safely. Weve already made oligos for millions of tests, and were scaling up even further. ...
Mouse TLR9-agonist kit; contains 100 ug of ODN1585-1; ODN1826-1, ODN2395-1, and negative controls ODN1585-1NC; ODN1826-1-1NC, ODN2395-1NC datasheet and description hight quality product and Backed by our Guarantee
Original Factory Issued Service Repair Manual for the Sansui Model TU-X701 DIGITAL SYNTHESIZER TUNER. Used, but in Very good condition. Printed in Japan
ALISO VIEJO, Calif., Oct. 4, 2016 /PRNewswire/ -- Microsemi Announces New Family of High Performance Frequency Synthesizers and Rate Converters. Leveraging...
The worlds first commercially available string synthesizer, the Eminent 310, came from an unlikely source, a Dutch home organ manufacturer. It was further advanced through an even more unlikely partnership, with the legendary American synth makers ARP...
List of all the English words with 24 letters beginning with O. octahydroxyanthraquinone, oligodeoxyribonucleotide, otorhinolaryngologically
Price: $4799.00; Manufacturer: Biotage; Item ID: 2029351; Warranty: 30-Day Money-Back Guarantee; Description: Biotage Initiator EXP
바이오닉스사는 1998년 Oligo synthesis service를 시작한 이래, 최신 설비와 노하우를 바탕으로 고품질 DNA Oligonucleotide를 생산/공급하고 있습니다. 특히 Global Oligo 제조공급사인 BIOBASIC®사로부터 지속적인 신규 장비와 기술을 도입하여 그 품질 향상을 도모하고 있으며, 이를 기반으로 업계 최고 수준의 품질을 자랑하는 Oligo와 Gene synthesis service 제공하고 있습니다 ...
Market Scenario:. The global market of oligonucleotide synthesis is growing at a rapid pace. The global oligonucleotide synthesis market is growing at the CAGR of around 10.3% for the forecasted period. The major factors that are influencing the market for oligonucleotide synthesis are increasing advancement in field of healthcare, increasing demand for innovation in the field of life science and medical academics, increasing investment by the government for the development of genomic technologies, increasing demand for oligonucleotide synthesis technologies by the public and private research firms. Furthermore increasing application of oligonucleotide synthesis in diagnostic, genetic testing, research, therapeutics, gene synthesis, library preparation, drug target screening, and others is further influencing the growth of the oligonucleotide synthesis market globally.. Key Players for Oligonucleotide Synthesis Market:. Integrated DNA Technologies, Inc (U.S), GE Healthcare (U.S.), Agilent ...
According to the new market research report The Report "Oligonucleotide Synthesis Market by Product & Services (Equipment, Reagent, Primer, Probe, Custom Oligos), End-User (Research, Pharmaceutical & Biotechnology), Application (Diagnostics, PCR, QPCR, Gene Synthesis, NGS, DNA, RNAi) - Global Forecast to 2019" provides a detailed overview of the major drivers, restraints, challenges, opportunities, current market trends, and strategies impacting the oligonucleotide synthesis market along with the estimates and forecasts of the revenue and competitive analysis.. This report studies the oligonucleotide synthesis market by products and services, applications, end users, and geography.. Oligonucleotide synthesis has a vast number of applications in nucleic acid array-based technologies, library preparations, next-generation sequencing methods, genomics, nucleic acid-based detection, cell cultures, diagnostics, therapeutics, human identity testing, cloning and genetic engineering, and synthetic ...
CpG-containing oligonucleotides (CpG) have been shown to reduce key features of allergic airway inflammation in mouse models. Given the inhibitory effects of CpG treatment on Ag presentation of subsequently encountered Ags via MHC class I and II molecules by dendritic cells (DC), we hypothesized that intranasal CpG treatment would lead to reduced Ag-specific T cell stimulation in the lung-draining lymph nodes, thereby reducing the inflammatory response in sensitized mice. Intranasal CpG administration led to phenotypic maturation of lung and mediastinal lymph node DC as determined by expression of MHC class II, CD80, and CD86. This was accompanied by a significant reduction in the proliferation of adoptively transferred Ag-specific CD4+ and CD8+ T cells in mediastinal lymph nodes, when CpG was given before inhalative OVA challenges. DC obtained from mediastinal lymph nodes of CpG-treated mice before OVA inhalation led to reduced T cell stimulation via MHC class I and II molecules. In addition, ...