TY - JOUR. T1 - In Vivo Response of Osteoblast-like and Odontoblast-like Cells in Intraperitoneal Diffusion Chamber. AU - Rodriguez, Andrea Paola. AU - Tsujigiwa, Hidetsugu. AU - Nagatsuka, Hitoshi. AU - Ichikawa, Kazuo. AU - Nagai, Noriyuki. AU - Minonishi, Atsuhisa. AU - Liliana, Missana. AU - Qin, C. L.. PY - 2005. Y1 - 2005. N2 - Preliminary analysis of cells before using them in tissue engineering is mandatory. Thus, to clarify the behavior of cells in an appropriate experimental model, we evaluated the characteristic of MDPC-23 cells and KUSA/A1 cells in vitro and in vivo seeded in diffusion chamber. Our results indicated that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber. Whereas, MDPC-23 cells were odontoblast-like cells but with low ability to induce dentin formation. This study suggests that MDPC-23 cells are special cells, which possess morphological and functional character of odontoblast-like cells, expressing DSPP only in vivo ...
TY - JOUR. T1 - The re-expression of nerve growth factor protein after cavity preparation in rat molars. AU - Shiomi, Hisashi. AU - Ohsaki, Yasuyoshi. AU - Akamine, Akifumi. AU - Iijima, Tadahiko. PY - 2000/12/1. Y1 - 2000/12/1. N2 - It is well known that nerve growth factor (NGF) is expressed in the odontoblasts of developing teeth, but not in completely grown teeth in rats. However, there have been no detailed studies of the expression of NGF in reactivated primary odontoblasts or in newly differentiated odontoblast-like cells during pulp repair. Therefore, to examine the involvement of NGF in odontoblasts during pulp repair, we immunohistochemically observed the localization of NGF in dental pulp after cavity preparation in rat molars. Based on the depth of the cavity, two types of pulp reaction were thus distinguished. 1) In shallow cavities, primary odontoblasts secreted a new dentin matrix, reactionary dentin, and also expressed NGF. 2) In deep cavities, odontoblast-like cells, ...
The pulp periphery: At the outer border of the pulp, odontoblasts and the so-called Hoehls cells form continuous layers. These postmitotic cells have the capacity to undergo terminal differentiation. Odontoblasts are implicated in the synthesis of collagen and noncollagenous extracellular matrix components. Some ECM proteins are phosphorylated (SIBLINGs), whereas others are non-phosphorylated. ECM components are implicated in predentin and dentin formation, followed by dentin mineralization. Due to a fixation artifact, the formation of a cell-free layer results in from fixation and dehydration. A cell-free area underlines odontoblasts and Hoehls cells, which do not appear on sections after adequate fixative perfusion. Fenestrated capillary loops infiltrate the layer formed by odontoblasts and Hoehls cells but do not cross the terminal junctions located between the distal odontoblast cell bodies nor penetrate within the predentin. In contrast, axons infiltrate the odontoblastic layer and ...
Odontoblasts published on PALAEONTOLOGY[online].... Odontoblasts are cells that form part of the centre of the tooth and are responsible for forming dentine.
The next layer of our teeth is called dentin. It underlies the enamel and surrounds the nerves of our teeth. Dentin is sensitive to touch and other stimuli. And dentin is holey, like swiss cheese. The holes are called dentinal tubules and they connect to odontoblasts that connect directly to the nerves. This was news to me-our teeth are naturally holey? Once the cementum is gone the dentin and the dentinal tubules (holes) and thus the nerves, are exposed to the elements. These holes generally cause no problems but they can if the odontoblasts move and irritate the nerve. Odontoblasts are particularly sensitive to fluid movements. Mr Dentist said that the sugar isnt directly hurting my teeth, rather the osmotic pressure of highly concentrated sugar is drawing water from the roots of my teeth which irritates the ondontoblasts and it is the pain of this osmosis that I am feeling. As soon as the sugar dissolves so does the pain. He described the root of the tooth as a little man whose head was ...
innermost: rich in fibroblasts and undifferentiated mesenchymal cells. middle: cell-free zone of Weil, rich in capillaries and nerves (nerve plexus located here). outermost layer: odontoblasts. Defense cells also found in pulp: macrophages, granulocytes, mast cells, plasma cells. ...
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Looking for online definition of odontoblast in the Medical Dictionary? odontoblast explanation free. What is odontoblast? Meaning of odontoblast medical term. What does odontoblast mean?
The odontoblasts of dental pulp tissue form primary and secondary dentine. If the pulp tissue is exposed, the odontoblast layer will be destroyed. The aim of direct pulp capping is to induce new hard tissue formation in order to keep the pulp tissue vital. If direct pulp capping is successful, tertiary dentine is formed. This hard tissue is denoted as reparative dentine and defined as a dentine matrix, which is formed by a new generation of odontoblast-like cells after an appropriate stimulus. Thus, reparative dentine is not formed by the original post-mitotic odontoblasts. Until recently it has been unclear which pulp cells differentiate into these odontoblast-like cells. Besides multipotent adult stem cells, fibroblasts and un-differentiated mesenchymal cells are mentioned in the literature as progenitor cells for odontoblast-like cells, because their cell-division rate increases significantly after direct pulp capping. The increase in the cell division rate of fibroblasts can be explained by ...
PubMed journal article Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in viv were found in PRIME PubMed. Download Prime PubMed App to iPhone, iPad, or Android
During normal pulp tissue healing, inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) or interleukins, act in the initial 48 hours (inflammatory phase) and play important roles not only as chemo-attractants of inflammatory cells and stem/progenitor cells but also in inducing a cascade of reactions toward tissue regeneration or reparative dentin formation or both. Previous reports have shown that inflammatory cytokines regulate the differentiation capacity of dental pulp stem/progenitor cells (DPCs), but none has interrogated the impact of these cytokines on the stem cell phenotype of stem/progenitor cells. This study investigated the effects of a short-term treatment with TNF-α on the stem cell phenotype and differentiation ability of human DPCs. An in vivo mouse model of pulp exposure was performed for analysis of expression of the mesenchymal stem cell marker CD146 in DPCs during the initial stage of inflammatory response. For in vitro studies, human DPCs were isolated and incubated
Dentine tooth tissue. Coloured scanning electron micrograph (SEM) of dentine (substantia eburnea), which is a mineralised connective tissue found under a tooths enamel. It forms the bulk of a tooth and differs from bone in its microscopic structure, which is seen here. Shown in cross-section are the dentinal tubules (dental canaliculi), which are where extensions from the odontoblast cells (part of the pulp at the core of a tooth) have formed the surrounding dentine matrix. These odontoblast extensions (or processes) allow dentine to rebuild itself, unlike enamel. Magnification: x460 when printed 10 centimetres wide. - Stock Image P486/0142
Our study provides initial experimental and clinical evidence that hDPSCs implanted into injured incisor teeth in the absence of a scaffold promoted 3D dental pulp regeneration and partial tooth recovery. The regenerated pulp tissue contained normal structures such as an odontoblast layer, connective tissue, blood vasculature, and neuronal tissue. The regenerated pulp in the incisor teeth receiving the hDPSC implants showed functional responses to a stimulus in the electric pulp test. Notably, compared to the apexification control group, the hDPSC implant group showed an increase in root length and a reduction in the width of the apical foramen at 12 months after treatment, suggesting that regenerated dental pulp promoted tooth recovery.. Given that tooth root development depends on the residual stem cells in the apical third of the root canal, several studies have assessed the effects of pulp revascularization treatment (23-25). This approach provides stem cells from tooth periapical tissue; in ...
Osteocalcin (OC) is a major non-collagenous protein synthesized by osteoblasts, odontoblasts and cementoblasts. We examined the function of OC in dentinogenesis by exposing rat and hamster tooth organ cultures to 1,25(OH)(2)vit D-3 or to bovine OC added to the culture medium. We furthermore examined dentinogenesis in tooth explants cultured in the presence of warfarin tan inhibitor of gamma-carboxylation of OC). Finally, we analyzed dentin from osteocalcin null mutant mice. Exposure to 1,25(OH)(2)vit D-3 increased OC synthesis by odontoblasts in vitro at the transcriptional and protein levels but had no apparent effect on matrix formation or Ca-45 uptake. High levels of bovine OC temporarily suppressed the initial formation of dentin and enamel and uptake of Ca-45. This effect was not seen when tooth explants were exposed to thermally decarboxylated OC. Exposure of tooth explants to warfarin had no significant effect on dentinogenesis. Dentin obtained from, two-month-old OC null mutants looked ...
Dentin is a yellow, calcified tissue, comprised of 70% mineral hydroxylapatite, 20% organic material and 10% water, produced by odontoblasts that are arranged in a layer at the pulp cavity of the tooth. Dentin is covered by enamel on the crown and cementum at the root, and affects tooth color due to the transparency of enamel. Odontoblasts send cellular projections into the mineralized dentin tissue, where they become enclosed within the channels called dentinal tubules and extend towards the outer wall of the dental pulp. Dentinal tubules also contain fluid that is thought to participate in the mechanisms responsible for sensation of pain and hypersensitivity ...
Kaše kamni - pulp stones lahko strukturno classi﫿ed in glede na mesto. Strukturno, so true in napačne celuloza kamni, tretja vrsta, amorfne celuloze kamni, več je nepravilne oblike kot napačne celuloza kamni, ki se pojavljajo v tesni povezavi z krvnih žil. Res celuloze kamni so iz dentina in podložene s odontoblasts, ker so napačne celuloza kamnov, sestavljeni iz izrodil celic celuloze, ki iskati rudnine. Take mineralizacije pojavi v fazah, sprva celico gnezda postane obdan s concentrically urejen 﫿bres, ki nato napojile z mineralnimi solmi. Glede na mesto, kaše kamni lahko vgrajeni, drži in brezplačno. Vdelani kamni so oblikovane v pulpi, vendar s stalno fiziološkega dentina tvorbo postane obdanim stene kanala. Najdemo jih najpogosteje v vršnem delu koren in prisotnost odontoblasts in calci﫿ed tkiva, ki spominja dentina lahko pojavijo na perifernih vidik teh kamnov. Priraslo celuloze kamni so preprosto manj pritrjen dentina kot vdelan celuloze kamni, razlika med drži in ...
PubMed journal article Differential expression patterns of the dentin matrix proteins during mineralized tissue formatio were found in PRIME PubMed. Download Prime PubMed App to iPhone or iPad.
Face To Face - Reactionary - Vinyl LP - 2000 - US - Original kaufen im Online Music Store von hhv.de - Neuheiten & Topseller auf Vinyl, CD & Tape - Versandkostenfrei bestellen ab 80€!
One of Americas most important contemporary artists, HANK WILLIS THOMAS delves into identity, race and consumer culture. In an interview excerpted from Roderick Stanleys GOOD TROUBLE, a biannual magazine that explores the intersection of art and culture with politics and protest, Thomas tells Francesca Gavin about the creation of history, our over-saturated image blindness, and the quest to be visionary, rather than reactionary.
The reactionary, rightwing Republican party exemplifies its bizarre disconnect to the real world with its blank denial of climate change… as large parts of the United States get torched from drought, soaring temperatures and wildfires.
As I had to work a lot with orthodontics at the last time I think I can say some things about this issue. To change the direction of a growing tooth, you need continuous forces (they should be present at least 16 hours a day to cause actual change of the tooth direction and surrounding bone). A single "accident" would hardly change the tooth in its direction, but either break it or burst it out of the bone, or, if the accident was not that serious, have no long-time-effect. I suppose the curvature of the teeth is just a result of different speed in the prodction of the odontoblasts within the root. The dentogenesis is actually a really weird thing, and as far a tooth has erupted, there is no more change of the crown possible (of course abrasion is possible). Orthodontics also have nearly no effect on the growth of the root itself, but mainly on the surrounding bone. The application of pressure causes reduction of bone tissue, and the application of pulling forces causes bone production. This can ...
Tideglusib is a pharmaceutical that is in clinical trials for systemic use in treating neurological disorders such as Alzheimers disease, according to the study authors.. After 24 hours, the treated teeth were extracted and measured for delivery of the antagonist. The researchers determined that this was an effective delivery method, so they moved on to the second phase, in which a different set of maxillary molars had the same size holes drilled, and soaked sponges were applied for four to six weeks.. The researchers used micro-CT scanning to view and quantify mineral deposition at the drill site. At both four and six weeks, the teeth showed statistically significant mineralization with all three agonists when compared with sponge-alone controls. They reported that mineralization was, on average, twice as great as in the control group.. The researchers then sectioned each molar and used a stain to show new dentine formation. These results confirmed the imaging data that showed that more ...
Background. The goal of treating exposed pulp with an appropriate pulp capping material is to promote the dentinogenic potential of the pulpal cells. There have been recent attempts to develop more effective pulp-capping materials. Objectives. The aim of this study was to evaluate the effect of newly developed calcium silicate-based material on odontogenic differentiation of primary human dental pulp cells (HDPCs), in comparison with a contemporary calcium silicate-based material. Material and methods. Human dental pulp cells isolated from dental pulps were cultured in standard culture conditions in Dulbeccos Modified Eagles Medium (DMEM) and then the effects of Micro-Mega mineral trioxide aggregate (MM-MTA) (Micro-Mega, Besancon, France) and ProRoot MTA (MTA) (Dentsply Sirona, Tulsa, USA) (positive control) were evaluated on HDPCs at 1, 7 and 14 days. Untreated cells were used as a negative control. Odontoblastic differentiation was assessed by alkaline phosphatase (ALP) activity. Runtrelated ...
Maintaining or regenerating a vital pulp is a preferable goal in current endodontic research. In this study, human dental pulp cell aggregates (spheres) were applied onto bovine and human root canal models to evaluate their potential use as pre-differentiated tissue units for dental pulp tissue regeneration. Human dental pulp cells (DPC) were derived from wisdom teeth, cultivated into three-dimensional cell spheres and seeded onto bovine and into human root canals. Sphere formation, tissue-like and mineralization properties as well as growth behavior of cells on dentin structure were evaluated by light microscopy (LM), confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). Spheres and outgrown cells showed tissue-like properties, the ability to merge with other cell spheres and extra cellular matrix formation; CLSM investigation revealed a dense network of actin and focal adhesion contacts (FAC) inside the spheres and a pronounced
Damage or exposure of the dental pulp requires immediate therapeutic intervention. This study assessed the biocompatibility of a silver-containing PLGA/TCP-nanofabric scaffold (PLGA/Ag-TCP) in two in vitro models, i.e. the material adapted on pre-cultured cells and cells directly cultured on the material, respectively. Collagen saffolds with and without hyaluronan acid (Coll-HA; Coll) using both cell culturing methods and cells growing on culture plates served as reference. Cell viability and proliferation were assessed after 24, 48, and 72 h based on formazan formation and BrdU incorporation. Scaffolds were harvested. Gene expression of interleukin(IL)-6, tumor necrosis factor (TNF)-alpha, and alkaline phosphatase (AP) was assessed 24 h after stimulation. In both models formazan formation and BrdU incorporation was reduced by PLGA/Ag-TCP on dental pulp cells, while no significant reduction was found in cells with Coll and Coll-HA. Cells with PLGA/Ag-TCP for 72 h showed similar relative BrdU
Dentinogenesis is a necessary prerequisite for dental tissue engineering. One of the steps for dentinogenesis is to obtain large quantities of highly purified odontoblasts. Therefore, we have undertaken an experiment applying different concentrations of beta-glycerophosphate (beta-GP) to induce the differentiation of dental pulp stem cells (DPSCs) in a long-term 28-day culture. In the meanwhile, we have studied the time-and maturation-dependent expression of matrix extracellular phosphoglycoprotein (MEPE) and that of the odontoblast-like marker-dentin sialoprotein (DSP), in order to investigate an optimized mineralized condition. Western blot results revealed that the expression of DSP became lower when accompanied by the increase of the beta-GP concentration, and there was also an influence on MEPE expression when different concentrations of beta-GP were applied. Meanwhile, the mineralized groups had an inhibitory function on the expression of MEPE as compared with the control group. Above all, ...
Dental pulp stem cells (DPSCs) are stem cells present in the dental pulp, the soft living tissue within teeth. They are multipotent, so they have the potential to differentiate into a variety of cell types. Other sources of dental stem cells are the dental follicle and the developed periodontal ligament. A subpopulation of dental pulp stem cells has been described as human immature dental pulp stem cells (IDPSC). There are various studies where the importance of these cells and their regenerative capacity has been demonstrated. Through the addition of tissue-specific cytokines, differentiated cells were obtained in vitro from these cells, not only of mesenchymal linage but also of endodermal and ectodermal linage. Among them are the IPS, MAPCs cells.[citation needed] Several publications have stressed the importance of the expression of pluripotentiality associated markers: the transcription factors Nanog, SOX2, Oct3/4, SSEA4, CD13, are indispensable for the stem cells to divide indefinitely ...
The dental pulp stem cell industry is a rapidly evolving industry. Every month there are major events occurring in the sector that shift industry dynamics. Often, these events are announcements of technical or scientific advancements. Sometimes they are announcements of major industry alliances. Occasionally, they are announcements of a new industry competitor, a major milestone, or a significant funding award.. For those of us interested in the dental pulp stem cell industry, tracking these shifting industry dynamics is of paramount importance. For this reason, this post covers the most significant neural stem cell industry news events as of July 2015.. [Read more…] ...
Dentin matrix protein 1 (DMP1) is an acidic extracellular matrix phosphoprotein that can bind calcium. DMP1 is required for bone and dentin mineralization and is expressed in the cells of bone and teeth. It is thought to play a role in regulating expression of osteoblast-specific genes during osteoblast cell differentiation and is localized to the nucleus in osteoblast precursor cells. In mature osteoblasts, the DMP1 protein is phosphorylated and localized to the extracellular matrix, where it plays a role in forming mineralized matrix. While other constitutively expressed proteins in the extracellular matrix, such as osteopontin and osteocalcin, are expressed in osteoblast cells, DMP1 is expressed in osteocytes, making it a candidate biomarker for osteocyte activity.. ...
Dentin matrix protein 1 (DMP1) is an acidic extracellular matrix phosphoprotein that can bind calcium. DMP1 is required for bone and dentin mineralization and is expressed in the cells of bone and teeth. It is thought to play a role in regulating expression of osteoblast-specific genes during osteoblast cell differentiation and is localized to the nucleus in osteoblast precursor cells. In mature osteoblasts, the DMP1 protein is phosphorylated and localized to the extracellular matrix, where it plays a role in forming mineralized matrix. While other constitutively expressed proteins in the extracellular matrix, such as osteopontin and osteocalcin, are expressed in osteoblast cells, DMP1 is expressed in osteocytes, making it a candidate biomarker for osteocyte activity.. ...
The transcription factor Nuclear Factor I-C (NFIC) has been implicated in the regulation of tooth root development, where it may be anticipated to impact on the behavior of stem cells from the apical papilla (SCAPs) and root odontoblast activity. We hypothesized that NFIC may provide an important target for promoting dentin/root regeneration. In the present study, the effects of NFIC on the proliferation and differentiation of SCAPs were investigated. Over-expression of NFIC increased cell proliferation, mineralization nodule formation and alkaline phosphatase (ALP) activity in SCAPs. Furthermore, NFIC up-regulated the mRNA levels of odontogenic-related markers, ALP, osteocalcin and collagen type I as well as dentin sialoprotein protein levels. In contrast, knockdown of NFIC by si-RNA inhibited the mineralization capacity of SCAPs and down-regulated the expression of odontogenic-related markers. In conclusion, the results indicated that upregulation of NFIC activity in SCAPs may promote ...
Human adult dental pulp stem cells enhance poststroke functional recovery through non-neural replacement mechanisms. - Wai Khay Leong, Tanya L Henshall, Agnes Arthur, Karlea L Kremer, Martin D Lewis, Stephen C Helps, John Field, Monica A Hamilton-Bruce, Scott Warming, Jim Manavis, Robert Vink, Stan Gronthos, Simon A Koblar
Canning thus suffers from what I have previously called "the primary delusion." He implicitly criticizes the independent determination that there is "little or no difference between the Republican and Democratic Parties," but ironically his solution to the progressive "electoral dilemma" is exactly the same as Rush Limbaughs solution to the conservative "electoral dilemma": work within the two-party system, change it from the inside via primary challenges. Is it still necessary to point out the fatal flaw in this strategy? Any political strategy predicated upon working within the confines of the Democratic and/or Republican Parties is incapable of addressing the single greatest political problem facing the people of the United States, progressive and conservative alike: the two-party state and the Democratic-Republican duopoly system of government. Waging a primary challenge is neither conservative nor progressive, it is reactionary accomodationism. In conceding to a primary challenge over ...
Fingerprint Dive into the research topics of Indirect immobilized Jagged1 suppresses cell cycle progression and induces odonto/osteogenic differentiation in human dental pulp cells. Together they form a unique fingerprint. ...
OBJECTIVE: This study aimed to investigate the effects of dodecacalcium hepta-aluminate (C12A7) content on some physicochemical properties and cytocompatibility of tricalcium silicate (C3S) cement using human dental pulp cells (hDPCs).MATERIAL AND METHODS: High purity C3S cement was manufactured by a solid phase method. C12A7 was mixed with the cement in proportions of 0, 5,
Cell viability rate (%) according to NR assay in human dental pulp cells (hDPCs) exposed to MTA Plus (MTAP), MTA Fillapex (MTAF), FillCanal (FC) and culture med
Principal Investigator:NAGASAKA Nobuo, Project Period (FY):1996 - 1997, Research Category:Grant-in-Aid for Scientific Research (B), Section:一般, Research Field:矯正・小児・社会系歯学
F. Carrouel, M. J Staquet, J. F Keller, C. Baudouin, P. Msika, F. Bleicher, B. Alliot-Licht, and J. C Farges (2013) J Endod, 39(8):1008-14.. ...
How are YouTube videos criticizing sexist video games important enough to threaten a school shooting? Read the #GamerGate tag and realize that underneath the anger is fear.
This is SUCH a weak argument against Atheism. First, throwing out the idea that were only about avoiding something arbitrarily, instead of with some good reasons. Then making a comparison, essentially, between religion and some other activity - usually an activity thats not very harmful, if at all. This seems to be an attempt to make us look like extremists and reactionaries, because why join a movement that doesnt have any deep driving reason to exist? Only extremists and reactionaries would do that, supposedly.. Theres no sense in comparing golf to organized religion. One is wasteful, and the other is not only profoundly wasteful on a much larger scale, but is harmful on a global scale. Were not avoiding something thats just there, like paint on a wall. Were trying to avoid the worlds worse affliction. Its so big and bad and harmful that yes, just the act of avoiding it in ones daily life warrants a label, a movement and a boatload of pride. Especially when we are the ones that ...
The United States is one of the few countries left in the world who still have not converted to using the Metric System as a standardized system of measurement. Instead of going along with what the rest of the world uses, we stubbornly still adhere to using the imprecise Imperial Unit - despite the fact that practically every other country that we interact with uses Metric ...
To ensure that your childs dental pulp stem cell collection goes smoothly, heres our guidance on how to prepare the tooth using our specialist sample collection kit
Sigma-Aldrich offers abstracts and full-text articles by [X V Tran, C Gorin, C Willig, B Baroukh, B Pellat, F Decup, S Opsahl Vital, C Chaussain, T Boukpessi].
In some ways, at least in East London, a new culture has grown up out of the multi-cultural society that has been born there over the last few years. Again, weve developed a local dialect, a local way of life and we still have a local market. To me this is positive. I think I was lucky to grow up in an area that produced such an exotic melting pot of cultural experience. But to the older generation, especially those who were moved out to Essex and the fertile BNP breeding ground that is Barking, its all very different from how it used to look and sound. The pace of change has overtaken their lives, and a lot of what they used to understand has disappeared. These are the people who have borne the price of the developments. Theyre the ones whose jobs have been swallowed up, undercut or shipped overseas. Theyre the ones who have lost the culture which represents their roots and sense of belonging. And out of that comes the bitterness, the resentment, the reactionary racism. Theyre lost in the ...
You can save the effort. You cant bait me. My last statement wasnt reactionary. Simply put, there are few places on Earth where making generalized statements against American lives, in public, wont get you into a lot of trouble. But, hey, its your choice. Just dont be suprised if youre called to account for it ...
Over the 1980s and 1990s we witnessed the simultaneous rise of two reactionary political projects, Hindutva and neoliberalism, to a position of dominance in…
Bernd Ulrich argued that Angela Merkels basic decision about the refugee policy in Germany was correct. You dont have to be a reactionary to see things differently.
Liberal researchers are taking wild flights of fancy in interpreting scientific data to marginalize conservatives as reactionaries.
Comrades, Some right-wing reactionary fiend, who no doubt thought he was being clever, sent me a copy of that horrid book Going Rogue written by ...
Objectives The aim of this study was to evaluate the level of odontogenic differentiation of dental pulp stem cells (DPSCs) on hydrogel scaffolds derived from bone extracellular matrix (bECM) in comparison to those seeded on collagen I (Col-I), one of the main components of dental pulp ECM. Methods DPSCs isolated from human third molars were characterized for surface marker expression and odontogenic potential prior to seeding into bECM or Col-I hydrogel scaffolds. The cells were then seeded onto bECM and Col-I hydrogel scaffolds and cultured under basal conditions or with odontogenic and growth factor (GF) supplements. DPSCs cultivated on tissue culture polystyrene (TCPS) with and without supplements were used as controls. Gene expression of dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1) and matrix extracellular phosphoglycoprotein (MEPE) was evaluated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and mineral deposition was observed by Von Kossa