DNA is a remarkable macromolecule that functions primarily as the carrier of the genetic information of organisms ranging from viruses to bacteria to eukaryotes. The ability of DNA polymerases to efficiently and accurately replicate genetic material represents one of the most fundamental yet complex biological processes found in nature. The central dogma of DNA polymerization is that the efficiency and fidelity of this biological process is dependent upon proper hydrogen-bonding interactions between an incoming nucleotide and its templating partner. However, the foundation of this dogma has been recently challenged by the demonstration that DNA polymerases can effectively and, in some cases, selectively incorporate non-natural nucleotides lacking classic hydrogen-bonding capabilities into DNA. In this review, we describe the results of several laboratories that have employed a variety of non-natural nucleotide analogs to decipher the molecular mechanism of DNA polymerization. The use of various non
Research, media articles and webinars on dietary nucleotides and its benefits on gut health, immune support, DNA repair and recovery. Restricted access for practitioners. Log-in to view.
(NaturalHealth365) Find out how to heal the digestive system naturally by getting enough dietary nucleotides. Plus, discover a way to improve immunity.
16) Devadoss, B., Lee, I., and Berdis, A. J. (2007) Is a thymine dimer replicated via a transient abasic site intermediate? A comparative study using non-natural nucleotides. Biochemistry 46, 4486-4498.(15) Lee, I. and Berdis, A. (2006) Fluorescent analysis of translesion DNA synthesis by using a novel, non-natural nucleotide analogue. ChemBioChem. 7, 1990-1997.. (14) Zhang, X., Donnelly, A., Lee, I., and Berdis, A. J. (2006) Rational attempts to optimize non-natural nucleotides for selective incorporation opposite an abasic site. Biochemistry 45, 13293-13303.. (13) Zhuang, Z., Berdis, A. J., and Benkovic, S. J. (2006) An alternative clamp loading pathway via T4 clamp loader gp44/62-DNA complex. Biochemistry 45, 7976-7989.. (12) Zhang, X., Zhou, X, Lee, I., and Berdis, A.J. (2005) Hydrophobicity, shape, and -electron density during translesion DNA synthesis. Journal of American Chemical Society, 128, 143-149.. (11) Zhang, X., Lee, I., and Berdis, A. J.(2005) The use of nonnatural nucleotides ...
This report describes the use of several isosteric non-natural nucleotides as probes to evaluate the roles of nucleobase shape, size, solvation energies, and π-electron interactions as forces influencing key kinetic steps of the DNA polymerization cycle. Results are provided using representative high- and low-fidelity DNA polymerases. Results generated with the E. coli Klenow fragment reveal that this high-fidelity polymerase utilizes hydrophobic nucleotide analogues with higher catalytic efficiencies compared to hydrophilic analogues. These data support a major role for nucleobase desolvation during nucleotide selection and insertion. In contrast, the low-fidelity HIV-1 reverse transcriptase discriminates against hydrophobic analogues and only tolerates non-natural nucleotides that are capable of hydrogen-bonding or π-stacking interactions. Surprisingly, hydrophobic analogues that function as efficient substrates for the E. coli Klenow fragment behave as noncompetitive or uncompetitive ...
TY - JOUR. T1 - Effect of the ε-subunit on nucleotide binding to Escherichia coli F1- ATPase catalytic sites. AU - Weber, Joachim. AU - Dunn, Stanley D.. AU - Senior, Alan E.. PY - 1999/7/2. Y1 - 1999/7/2. N2 - The influence of the ε-subunit on the nucleotide binding affinities of the three catalytic sites of Escherichia coli F1-ATPase was investigated, using a genetically engineered Trp probe in the adenine-binding subdomain (β-Trp-331). The interaction between ε and F1 was not affected by the mutation. K(d) for binding of ε to βY331W mutant F1 was ~1 nM, and ε inhibited ATPase activity by 90%. The only nucleotide binding affinities that showed significant differences in the ε-depleted and ε-replete forms of the enzyme were those for MgATP and MgADP at the high-affinity catalytic site 1. K(d1)(MgATP) and K(d1)(MgADP) were an order of magnitude higher in the absence of ε than in its presence. In contrast, the binding affinities for MgATP and MgADP at sites 2 and 3 were similar in the ...
The guanine nucleotide binding properties of rap1 protein purified from human neutrophils were examined using both the protein kinase A-phosphorylated and the non-phosphorylated forms of the protein. Binding of GTP[S] (guanosine 5′-[gamma-thio]triphosphate) or GDP was found to be slow in the presence of free Mg2+, but very rapid in the absence of Mg2+. The binding of guanine nucleotides was found to correlate with the loss of endogenous nucleotide from the rap1 protein, which was rapid in the absence of Mg2+. The relative affinities of GTP and GDP for the binding site on rap1 were modulated by the presence of Mg2+, with a preferential affinity (approx. 15-fold) for GTP observed only in the absence of this bivalent cation. The dissociation of GDP from rap1 was not affected by the G-protein beta/gamma-subunit complex. Phosphorylation of rap1 in vitro by protein kinase A did not modify any of the observed nucleotide-binding parameters. Furthermore, the ability of a cytosolic rap1 ...
gs99, invoking micro-intelligence isnt needed to explain the arrival of activated nucleotides during replication. The Polymerase doesnt send out for the right nucleotide triphosphate; instead, random nucleotide triphosphates bump into the template nucleotide. If the nucleotide triphosphate hydrogen bonds correctly with the template nucleotide, the new phosphodiester linkage forms. If the nucleotide triphosphate is the wrong one then usually it will not slip into the correct orientation to form the new bond, so it is quickly displaced and another random activated nucleotide slips into position at the polymerase active site and bumps against the template nucleotide. Eventually (in a tiny fraction of a second) a correct nucleotide is fitted and reacted and the polymerase advances. If a mistake is made (and this sometimes happens), it is almost always caught by the proofreading function of the polymerase and corrected. If not, a mutation has occurred (a rare event). The process is driven by ...
gs99, invoking micro-intelligence isnt needed to explain the arrival of activated nucleotides during replication. The Polymerase doesnt send out for the right nucleotide triphosphate; instead, random nucleotide triphosphates bump into the template nucleotide. If the nucleotide triphosphate hydrogen bonds correctly with the template nucleotide, the new phosphodiester linkage forms. If the nucleotide triphosphate is the wrong one then usually it will not slip into the correct orientation to form the new bond, so it is quickly displaced and another random activated nucleotide slips into position at the polymerase active site and bumps against the template nucleotide. Eventually (in a tiny fraction of a second) a correct nucleotide is fitted and reacted and the polymerase advances. If a mistake is made (and this sometimes happens), it is almost always caught by the proofreading function of the polymerase and corrected. If not, a mutation has occurred (a rare event). The process is driven by ...
In Genome Research this week: transcriptome interrogation at single-nucleotide resolution with RNA-seq, three-level genome annotations, analyzing P450s in the human liver, and more.
Chakraborty S, Krishnan Y. 2017. A structural map of oncomiR-1 at single-nucleotide resolution.. Nucleic Acids Res. 45(16):9694-9705. ...
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Using in silico analysis tools, we compiled Arabidopsis nuclear mRNA poly(A) signals from two independently produced 3′-UTR datasets covering about 17,000 independent genes. Beyond confirming the previous working model on the NUE and FUE, we revealed complex nucleotide distribution patterns around the CS and poly(A) site. The signal surrounding the CS is named CE here. A set of prevailing, although not highly conserved, patterns that are potentially poly(A) signals for each of the three elements are presented. Conserved secondary structures surrounding the CSs were also predicted using the RNA secondary structure prediction program, mFold. Using data from the literature, it is confirmed that these structures are important for the functionality of the signals because only those mutations that altered secondary structures had impact on the efficiency of the signals. These findings should serve as a new starting point for plant poly(A) signal study, e.g. the basis for mutagenesis tests of CE, the ...
Biology Assignment Help, What is nucleotides, What is Nucleotides? Nucleotides are the basic building blocks of nucleic acids. Each nucleotide is composed of a nitrogen base, a 5-carbon sugar called a pentose, and an associated phosphate group. The nitrogen bases are composed of either
TrkH is a bacterial ion channel implicated in K uptake and pH regulation. TrkH assembles with its regulatory protein, TrkA, which closes the channel when bound to ADP and opens it when bound to ATP. However, it is unknown how nucleotides control the gating of TrkH through TrkA. Here we report the structures of the TrkH-TrkA complex in the presence of ADP or ATP. TrkA forms a tetrameric ring when bound to ADP and constrains TrkH to a closed conformation. The TrkA ring splits into two TrkA dimers in the presence of ATP and releases the constraints on TrkH, resulting in an open channel conformation. Functional studies show that both the tetramer-to-dimer conversion of TrkA and the loss of constraints on TrkH are required for channel gating. In addition, deletion of TrkA in Escherichia coli depolarizes the cell, suggesting that the TrkH-TrkA complex couples changes in intracellular nucleotides to membrane potential ...
Nucleotides can be synthesized through a variety of methods both in vitro and in vivo. This can involve salvage synthesis (the re-use of parts of nucleotides in resynthesizing new nucleotides through breakdown and synthesis reactions in order to exchange useful parts), or the use of protecting groups in a laboratory. In the latter case, a purified nucleoside or nucleobase is protected to create a phosphoramidite, and can be used to obtain analogues not present in nature and/or to create an oligonucleotide. ...
To conquer this problem, we calculated one nucleotide turnovers by first incubating the fiber in a comforting resolution that contained 250 μM mantATP. MantATP
free nucleotides of adenine/A bond only with thymine/T / vice versa and free nucleotides of cytosine/C bond only with guanine/G / vice versa; thus copying the opposite strand of the original DNA molecule ...
Nucleotides may help maintain the optimum rate of cell replacement and help support the immune system. Quality Nucleotide supplements plus free UK delivery.
Finds sub-sequences or patterns in the sequence and highlights the matching regions. The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. More... ...
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English: Nucleotides are nucleosides with one or more phosphate group. They may also contain diphosphate and triphosphate groups ...
Nucleotides are involved in the most cellular processes and play an important role in the structural and regulatory functions in the body
A nucleotide is an organic molecule that is the building block of DNA and RNA. They also have functions related to cell signaling, metabolism, and enzyme reactions
The Nucleotide database is a collection of sequences from several sources, including GenBank, RefSeq, TPA and PDB. Genome, gene and transcript sequence data provide the foundation for biomedical research and discovery ...
Finds sub-sequences or patterns in the sequence and highlights the matching regions. The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. More... ...
Finds sub-sequences or patterns in the sequence and highlights the matching regions. The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. More... ...
You may be asked to name the three parts of a nucleotide and explain how they are connected or bonded to each other. Here's the answer for both DNA and RNA.
|p class=Fliess|Nucleotides:|br /|Nucleotides are the primary building blocks of cell replication (RNA and DAN). Whenever cells need to be re-formed, such as in the formation of white blood cells during an immune response, nucleotides play a central rol
The likely nucleotide content of our modern Western diet has changed considerably when compared to that of our grandparents. If we go back even further in time to before the domestication of animals and plants and the industrialisation of agriculture and food production methods we find the changes to be even more significant.. A selection of results from recent investigations into the nucleotide content of a range of different foodstuffs is shown in figure 1 below. Based on the analysis of freeze-dried samples by High Performance Liquid Chromatography (HPLC) the total nucleotide content for a typical adult portion size is shown in each case.. The results reveal some dramatic differences in the likely nucleotide intake of meat versus vegetarian diets, pointing to the highest contents in the offal-type (or organ) meats far less likely to be found on dinner plates today.. ...
With SeqCap Epi you can choose your own content or select the CpGiant Enrichment Kit. This off-the-shelf probe-set interrogates over 5.5 million methylation sites per sample at single-nucleotide resolution. This kit can serve as a screening tool to identify specific regions in the genome for methylation variation assessment. Capturing of known differentially methylated regions vastly reduces the sequencing output required to analyze the whole methylation landscape, compared to whole genome sequencing. Furthermore, it enables you to observe the methylation changes on both strands, while you can still study the SNPs in the targeted regions.. ...
ATP binding cassette (ABC) transporters mediate vital transport processes in every living cell. ATP hydrolysis, which fuels transport, displays positive cooperativity in numerous ABC transporters. In particular, heterodimeric ABC exporters exhibit pronounced allosteric coupling between a catalytically impaired degenerate site, where nucleotides bind tightly, and a consensus site, at which ATP is hydrolyzed in every transport cycle. Whereas the functional phenomenon of cooperativity is well described, its structural basis remains poorly understood. Here, we present the apo structure of the heterodimeric ABC exporter TM287/288 and compare it to the previously solved structure with adenosine 5-(β,γ-imido)triphosphate (AMP-PNP) bound at the degenerate site. In contrast to other ABC exporter structures, the nucleotide binding domains (NBDs) of TM287/288 remain in molecular contact even in the absence of nucleotides, and the arrangement of the transmembrane domains (TMDs) is not influenced by ...
deoxy)ribose unit and the base, a β -glycosidic bond, has a different connectivity according to whether the link is to a purine or a pyrimidine. Although the linkage involves a C1′ carbon in both cases, the β -glycosidic bond in the case of a purine nucleotide is a link to the N-9 of the purine base , but to the N-1 of the pyrimidine base . Nucleotides are also found in which two or three phosphate groups are linked together, the chain of phosphate groups bonded to the sugars 5′-position. In these cases, they are nucleoside diphosphates (5′-NDP) and nucleoside triphosphates (5′-NTP). The bases have very limited solubilities in water, whereas the nucleosides and nucleotides have greater solubilities, due to the presence of polar sugars, or of both sugars and charged phosphate groups, respectively. The nucleoside triphosphates are of special interest for at least two reasons. First, they are the actual precursor molecules used in the biosynthesis of nucleic acids. Second, ATP is a ...
Detection of variable nucleotide(s) is based on primer extension and incorporation of detectable nucleoside triphosphates. By selecting the detection step primers from the region immediately adjacent to the variable nucleotide, this variation can be detected after incorporation of as few as one nucleoside triphosphate. Labelled nucleoside triphosphates matching the variable nucleotide are added and the incorporation of a label into the detection step primer is measured. The selection of the detection step primer is important to the method according to this invention and is dependent on the nucleotide sequence of interest. The detection step primers are preferably selected so to span the region immediately toward the 3 end from the variable nucleotide to be detected. The method is useful in identifying specific point mutations and genetic variations.
0 votes . Nucleotides also serve as an energy source. A. B. They are building blocks of nucleic acid, as nucleotides consist of the same components such as a nitrogenous base, sugar and a phosphate group. ? Hence, by adding a phosphate group, a nucleoside can be converted into a nucleotide by the enzymes called kinase. Cytosine, thymine and uracil are pyrimidine bases. 1800-212-7858 / 9372462318. … There are mainly two groups of nitrogenous bases such as purines and pyrimidines. This is because nucleotides are the building blocks of nucleic acids and certain nucleotides serve as the energy currency of the cell. On the contrary, when the pentose sugar is deoxyribose, the forming polynucleotide is called as DNA. Nucleotide is the building block of two crucial macromolecules (nucleic acids) in living organisms called DNA and RNA. 176 views. Nucleoside: A nucleoside is the precursor of nucleotide. Biology. 4. Nucleoside: Several nucleoside analogues are used as antiviral or anticancer agents. ...
Use TriLink biotin monophosphates for functionalization of RNA through in vitro transcription. Use our biotin dNTPs for efficient uniform labeling in PCR.. Labeled nucleoside monophosphates enable capture and enrichment during procedures such as aptamer development by SELEX. For example, use of 5-biotin-labeled nucleotides in in vitro transcription reactions yield transcribed aptamers containing biotin at the 5 terminus, which allows their subsequent immobilization on a avadin-coated chip.. Labeled nucleoside triphosphates can be incorporated into nucleic acids during amplification, such as PCR or in vitro transcription, to incorporate a biotin handle for use in affinity capture/purification, hybrid capture, immunoassays, tracer labeling, and nucleic acid detection. Our biotinylated nucleoside triphosphates are modified in positions that will not affect the ability of the base to pair with its partner. We also offer dye-labeled nucleoside triphosphates for incorporation into and fluorescent ...
Among these four types of SNPs, C ↔ T/A ↔ G, A ↔ C/G ↔ T, A ↔ T, and C ↔ G, the neighborhood patterns of nucleotide distributions were, however, quite different from each other (Figure 2). For example, at the −1 site, the trend of nucleotide dynamics was A , T , C , G for the combined C ↔ T/A ↔ G transitions (Figure 2, A and B), T , A , G , C for the combined A ↔ C/G ↔ T transversions (Figure 2, C and D), T , A , C , G for the A ↔ T transversions (Figure 2E), and A , T , G , C for the C ↔ G transversions (Figure 2F), respectively. However, at the +1 site, the trend was G , A , T , C for the combined C ↔ T/A ↔ G transitions (Figure 2, A and B), T , A , G , C for the combined A ↔ C/G ↔ T transversions (Figure 2, C and D), A , T , G , C for the A ↔ T transversions (Figure 2E), and T , A , C , G for the C ↔ G transversions (Figure 2F), respectively.. For the C ↔ T/A ↔ G transitions (Figure 2, A and B), nucleotide A had a high average frequency of 0.3548 ...
They are composed of nucleotides, which are the monomers made of three components: a 5-carbon sugar, a phosphate group and a nitrogenous base. Nucleic Acids - Structure and Function. Normally, they are not needed in the diet. The formation of the polymeric nucleotides follows the polyester synthesis principle. Natural & modified nucleotides sorted by chemical structure motifs. DNA Definition and Structure . Each nucleotide is a polymer made up of three parts: A five-carbon sugar (2-deoxyribose in DNA or ribose in RNA) A phosphate molecule; A nitrogenous (nitrogen-containing) base ARN (acide ribonucléique) ou RNA (ribonucleic acid). nucleic-acids. So the two pyrimidines for RNA are cytosine and uracil (green and pink beads). i include the base thymine. Adenosine diphosphate (ADP) and adenosine triphosphate (ATP), shown in Figure 19.4 Structures of Two Important Adenine-Containing Nucleotides, have a role in cell metabolism that we will discuss in Chapter 20 Energy Metabolism. The supposed ...
Try NEN Life Science Products at: http://www.nenlifesci.com for analogs list under Renaissance Nonrad. Nucleotides where there are over 100 different fluorescent and hapten labeled nucleotides as riboNTPs (except GTP early 1999), deoxyNTPs and dideoxyNTPs. Philip R. Buzby, Ph.D. Nonrad. Nucleotide R&D Phone: 617-350-9343 NEN Life Science Products, Inc. Voice Mail: 800-446-0035, 1, 9343 PO BOX 199151 FAX: 617-350-9658 Boston, MA 02119 E-Mail: buzbypr at nenlifesci.com ...
Pippucci T, Savoia A, Perrotta S, Pujol-Moix N, Noris P, Castegnaro G, Pecci A, Gnan C, Punzo F, Marconi C, Gherardi S, Loffredo G, De Rocco D, Scianguetta S, Barozzi S, Magini P, Bozzi V, Dezzani L, Di Stazio M, Ferraro M, Perini G, Seri M, Balduini CL (2011) Mutations in the 5 UTR of ANKRD26, the ankirin repeat domain 26 gene, cause an autosomal-dominant form of inherited thrombocytopenia, THC2. Am J Hum Genet 88(1):115-120. https://​doi.​org/​10.​1016/​j.​ajhg.​2010.​12.​006 CrossRefPubMedPubMedCentral ...
Définitions de Nucleotide salvage, synonymes, antonymes, dérivés de Nucleotide salvage, dictionnaire analogique de Nucleotide salvage (anglais)
Nucleotides contain a high and very variable proportion of water. Vitablend assays each batch of incoming nucleotide raw material and adjusts the premix formulation for every batch produced in accordance with those assay results. Our approach guarantees a low tolerance nucleotide profile blend compared to a typical approach using a fixed recipe of raw materials ...
In article ,[email protected][144.92.64.174],, mlsulliv at facstaff.wisc.edu (Michael L. Sullivan) wrote: , ,Something I would check would be the pH of the dNTP stock using pH paper. ,pH should be ,7, I think. If the solution is acid, I think the dNTPs ,disproparionate into dNDP and dNtetraP. If the pH is acid the purine nucleotides will depurinate (the adenine or guanine moiety will part company with the ribose triphosphate part of the dATP or dGTP). According to what I read many years ago in the then Pabst Laboratories catalog (a division of Pabst brewery--brewers have yeast, and yeast is a source of ATP;later the company was called PL laboratories, and still later Pharmacia(?)), disproportionation is only a problem in the *solid* state. According to PL, solid nucleotide triphosphates should be kept at -80 C, but the solutions are stable at -20 C. Makes me wonder about the 5-gm bottles of ATP that seem to be in a freezer in every laboratory. -- Ned Mantei Department of Cell Biology, ...
The technology science behind next-generation sequencing is varied and can get complicated, but heres an overview. It relies on an ingenious method of identifying different nucleotides, which are the building blocks of DNA: adenine (A), cytosine (C), guanine (G), and thymine (T). Sequencing by synthesis technology can identify the different nucleotides by examining the light they emit after being exposed to a laser. The four nucleotides have a specific fluorescent molecule that emits a unique wavelength of light when its hit by the laser, so A, G, C, or T can be identified separately. A sophisticated camera captures this light, recording the overall order (sequence) of the nucleotides. This information is stored in a form that researchers can use to compare pieces of DNA and create vast libraries of genes for any type of living creature that carries DNA ...
Ein. Consequently, MAG_5040 could be a critical pathogenic contributor to M. agalactiae persistence by providing essential nucleotide precursors for
Apyrases are active against both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyse NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates. They differ from ATPases, which specifically hydrolyse ATP, by hydrolysing both ATP and ADP. The eukaryotic enzymes requires Ca2+, but Mg2+ can substitute. Most of the ecto-ATPases that occur on the cell surface and hydrolyse extracellular nucleotides belong to this enzyme family ...
Effect of Glutamine, Glutamic Acid and Nucleotides on the Turnover of Carbon (δ13C) in Organs of Weaned Piglets - Liver;Pancreas;Pigs;Stable Isotopes;
Nucleotide degradation is an integrated process in all human cells that is intimately linked with the pathways of nucleotide synthesis and salvage
Nucleotides, in addition to performing a number of independent function in cells, are the basic units of nucleic acids (DNA and RNA). They include a sugar, one to three phosphate groups, and a base. The nucleoside structure is simply a nucleotide stripped of its phosphate group(s).
A salvage pathway is a pathway in which nucleotides (purine and pyrimidine) are synthesized from intermediates in the degradative pathway for nucleotides. Salvage pathways are used to recover bases and nucleosides that are formed during…
23DD Nucleotide Set K-1004 2,3-Dideoxynucleotide Kit (DNA chain terminators)Contains 1.0 umole each of 4 nucleotides: -N-4002 2,3-Dideoxyguanosine-5-Tri
Nucleotide, any member of a class of organic compounds in which the molecular structure comprises a nitrogen-containing unit (base) linked to a sugar and a phosphate group. The nucleotides are of great importance to living organisms, as they are the building blocks of nucleic acids, the substances
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Start typing in the text box, then select your taxid. Use the plus button to add another organism or group, and the exclude checkbox to narrow the subset. The search will be restricted to the sequences in the database that correspond to your subset. ...
Start typing in the text box, then select your taxid. Use the plus button to add another organism or group, and the exclude checkbox to narrow the subset. The search will be restricted to the sequences in the database that correspond to your subset. ...
Welcome to Nucleotides for Health your place on the web for information on or about nucleotides, immunity, figestive health, endurance, research and development .....
Communicated by Olle E. Bjorkman, Carnegie Institution of Washington, Stanford, CA, December 9, 2003 (received for review June 12, 2003) ArticleFigures SIInfo however, where indicated, pea and Arabidopsis were used. Intact pea chloroplasts
1A4R: Nucleotide binding to the G12V-mutant of Cdc42 investigated by X-ray diffraction and fluorescence spectroscopy: two different nucleotide states in one crystal.
Tool: bedtools nuc (aka nucBed) Version: v2.16.2 Summary: Profiles the nucleotide content of intervals in a fasta file. Usage: bedtools nuc [OPTIONS] -fi ,fasta, -bed ,bed/gff/vcf, Options: -fi Input FASTA file -bed BED/GFF/VCF file of ranges to extract from -fi -s Profile the sequence according to strand. -seq Print the extracted sequence -pattern Report the number of times a user-defined sequence is observed (case-sensitive). -C Igore case when matching -pattern. By defaulty, case matters. Output format: The following information will be reported after each BED entry: 1) %AT content 2) %GC content 3) Number of As observed 4) Number of Cs observed 5) Number of Gs observed 6) Number of Ts observed 7) Number of Ns observed 8) Number of other bases observed 9) The length of the explored sequence/interval. 10) The seq. extracted from the FASTA file. (opt., if -seq is used) 11) The number of times a users pattern was observed. (opt., if -pattern is used ...
A building block of DNA or RNA- nucleotide is made up of the sugar, nitrogenous bases and phosphate while the nucleoside is made up of sugar and bases only.