Importin and nucleoplasmin complex, molecular model. Nucleoplasmin tags proteins for entry to the cell nucleus, while importin transports the protein across the nuclear membrane. - Stock Image C025/1566
HI-TECH SOLUTIONS - Exporter, Importer, Manufacturer, Distributor & Supplier of Nuclear Chromatin Decondensation (N.C.D.) based in New Delhi, India
KPNA4 (karyopherin alpha 4), also known as importin alpha 3, is a member of the importin alpha family of proteins which includes KPNA1 (importin alpha…
We present depth-resolved spatial-domain low-coherence quantitative phase microscopy, a simple approach that utilizes coherence gating to construct a depth-resolved structural feature vector quantifying sub-resolution axial structural changes at different optical depths within the sample. We show that this feature vector is independent of sample thickness variation, and identifies nanoscale structural changes in clinically prepared samples. We present numerical simulations and experimental validation to demonstrate the feasibility of the approach. We also perform experiments using unstained cells to investigate the nanoscale structural changes in regulated cell proliferation through cell cycle and chromatin decondensation induced by histone acetylation. (C) 2013 Optical Society of ...
While there have been a couple of other single player Half-Life mods that incorporate playing as a Xen alien (Xen Warrior) or fighting on the side of the Xen aliens (Mission of Mercy), it has taken until now for a mod to really do it right. Point of View casts you as Xonxt the super worker, which is a step above a normal alien slave. The designers went all the way on this mod; you have new alien weapons (including the classic slave green-lightning attack), you make alien sounds, and you even bleed yellow alien blood. The little professional touches like these, combined with the fairly solid gameplay, design, and a detailed story, make Point of View a mod worth the download.. POV comes to us from the same core team that developed Azure Sheep, Andrea "Toadman" Butti and Davide "DAV" Cintrao. In fact, if youve played Azure Sheep youll notice quite a bit of crossover between these two mods, both in characters/enemies and in locations/sequences. POV ties in nicely with scenes from the original ...
This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008 ...
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KPNA2兔多克隆抗体(ab82313)可与人样本反应并经WB, ELISA实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
1EE5: Crystallographic analysis of the specific yet versatile recognition of distinct nuclear localization signals by karyopherin alpha.
The FKBP39 from Drosophila melanogaster is a multifunctional regulatory immunophilin. It contains two globular domains linked by a highly charged disordered region. The N-terminal domain shows homology to the nucleoplasmin core domain, and the C-terminal domain is characteristic for the family of the FKBP immunophilin ligand binding domain. The specific partially disordered structure of the protein inspired us to investigate whether FKBP39 can drive spontaneous liquid-liquid phase separation (LLPS). Preliminary analyses using CatGranule and Pi-Pi contact predictors suggested a propensity for LLPS. Microscopy observations revealed that FKBP39 can self-concentrate to form liquid condensates. We also found that FKBP39 can lead to LLPS in the presence of RNA and peptides containing Arg-rich linear motifs derived from selected nuclear and nucleolar proteins. These heterotypic interactions have a stronger propensity for driving LLPS when compared to the interactions mediated by self-associating FKBP39 ...
Plasmid pCMVTNT-T7-KPNA2 from Dr. Bryce Paschals lab contains the insert Karyopherin Alpha 2 and is published in BMC Cell Biol. 2010 . 11():63. This plasmid is available through Addgene.
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B138 Background: Treatment of breast cancer cells with histone deacetylase (HDAC) inhibitors results in chromatin decondensation and the sensitization of cancer cells to DNA damaging agents such as topoisomerase II inhibitors. We previously reported that the HDAC inhibitors induced chromatin decondensation through the down-regulation of heterochromatin maintenance proteins such as heterochromatin protein 1 (HP1), structural maintenance of chromatin proteins (SMC) 1-5 and DNA methyltransferase 1 (DNMT1). Here we report the role of HDAC2 in the expression of heterochromatin maintenance proteins, chromatin decondensation and DNA damage induced by topoisomerase inhibition. Methods: HDAC2 was selectively depleted using siRNA transfection. HDAC2 depleted cells were evaluated by microarray and Western blot analysis for changes in HP1, DNMT1 and SMC mRNA and protein expression. Chromatin decondensation was evaluated by electron microscopy. DNA damage and cell death induced by the topoisomerase ...
Exposure of mammalian cells to agents that induce apoptosis results in a rapid and substantial inhibition of protein synthesis. In MCF-7 breast cancer cells, tumor necrosis factor alpha (TNFalpha) and TNF-related apoptosis-inducing ligand inhibit overall translation by a mechanism that requires caspase (but not necessarily caspase-3) activity. This inhibition is associated with the increased phosphorylation of eukaryotic initiation factor (eIF2) alpha, increased association of eIF4E with the inhibitory eIF4E-binding protein (4E-BP1), and specific cleavages of eIF4B and eIF2alpha. All of these changes require caspase activity. The cleavage of eIF4GI, which specifically needs caspase-3 activity, is dispensable for the inhibition of translation in MCF-7 cells. Similar experiments with embryonic fibroblasts from control mice and animals defective for expression of the double-stranded RNA-regulated protein kinase (PKR) reveal requirements for both caspase activity and PKR for inhibition of protein ...
FISH studies on several strongly transcribed chromosomal regions have shown a disposition for looping out from their respective chromosome territories (Mahy et al., 2002b; Volpi et al., 2000; Williams et al., 2002), suggesting a large-scale chromatin decondensation reminiscent of results obtained by targeting transcription factors to transgene arrays. In the first of these targeting studies chromatin decondensation was induced by the viral transcriptional VP16 acidic activation domain. Targeting was achieved within the context of large transgene arrays containing multiple-copy plasmid integrations; each plasmid carried direct repeats of 256 (Tumbar et al., 1999) or 96 (Tsukamoto et al., 2000) operator binding sites for fusion proteins between the lac or tet repressor and VP16. Despite the large opening activity observed, the biological relevance of these observations hinges on the actual physiological relevance of the experimental system. In particular, there are three obvious concerns.. First, ...
Shikhar Uttam,1,* Rajan K. Bista,1 Kevin Staton,1 Sergey Alexandrov,2 Serah Choi,3 Christopher J. Bakkenist,3 Douglas J. Hartman,4 Randall E. Brand,5 and Yang Liu1 1Biomedical and Optical Imaging Laboratory (BOIL), Departments of Medicine and Bioengineering, University of Pittsburgh, Pittsburgh, PA 15213, USA 2Tissue Optics and Microcirculation Imaging Group (TOMI), School of Physics, National University of Ireland, Galway, Ireland 3Department of Radiation Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15232, USA 4Department of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA 5Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA *Corresponding author: [email protected] ...
GAMS provides many functions, ranging from commonly used standard functions like exponentiation, logarithms, and trigonometric functions to utility functions for controlling and querying the running GAMS job or process. The complete list of available functions is given in the following tables: Mathematical Functions, String Manipulation Functions, Logical Functions, Time and Calendar Functions, and GAMS Utility and Performance Functions. For details specific to using these functions in equations, see the section on Expressions in Equation Definitions.. Some of the tables that follow contain an endogenous classification column "End. Classif." that specifies in which models the function may legally appear. In order of least to most restrictive, the choices are DNLP, NLP, any, none. See section Classification of Models for details on model types in GAMS. Note well: functions classified as any are only permitted with exogenous (constant) arguments.. Functions are typically used in assignment ...
DNA calls for the return of the nuclear envelope (NE) after mitosis, according to Ulbert et al. (page 469). The abundance of DNA may thus be one reason why the NE reforms so quickly.. NE reformation occurs so quickly that breaking down the process in vivo has so far been impossible. Many scientists instead use in vitro reconstitution assays, which indicate that chromatin decondensation (as occurs at the end of mitosis) initiates the recruitment of vesicle populations to chromatin. In the new work, the authors aimed to identify membrane and chromatin components that mediate this recruitment and thus NE assembly.. The main component of chromatin is, of course, DNA. The group found that excess naked DNA prevented in vitro NE reformation by titrating vesicles away from chromatin at early stages. DNA alone was a more potent inhibitor than chromatin, although chromatin proteins might also contribute, particularly later on as the envelope matures.. DNA does not bind pure liposomes, so the authors next ...
The key finding of this study is that the permeability of the yeast NE is greatly affected by the deletion of NUP170 and NUP188. These results establish the role of Nup170p and Nup188p in determining the functional diameter of the diffusion channel which, at ∼10-nm diameter, is the largest known channel in nature (Zimmerberg 1999). Although the hypothesis that the diffusion channel is part of the NPC is considered fact, there is actually no genetic or biochemical evidence to support it. The present results are the first to directly link particular NPC components to the diffusion channel.. The diffusion channel is an enigma. Many karyophilic proteins that are small enough to diffuse across the NE are imported by receptor-mediated pathways (Breeuwer and Goldfarb 1990; Pruschy et al. 1994). What, if not for the transport of small proteins, is the function of the ∼10-nm diameter diffusion channel? A much smaller channel would suffice for the diffusion of ions, metabolites, and nucleotides. There ...
Contrary to previous work, Dreier and Rapoport show on page 883 that microtubules are not required for the in vitro remodeling of membranes into ER-like tubules. Dreier and Rapoport began by attempting to dissect an in vitro nuclear envelope formation reaction in frog egg extracts. But the reaction was complicated by associated chromatin decondensation and remodeling reactions and, in the meantime, the researchers observed that the reaction conditions yielded ER-like membrane structures. (In any case, success with the simpler ER formation assay may be applicable to nuclear envelope formation, as the two reactions seem to share many characteristics.) The reaction requires only a light membrane fraction and cytosol. By itself, a washed membrane fraction fuses to form larger vesicles but does not form tubules.. ...
Importin alpha 5/KPNA1/SRP1 products available through Novus Biologicals. Browse our Importin alpha 5/KPNA1/SRP1 product catalog backed by our Guarantee+.
importin-alpha-P1, importin alpha 1, IPOA1, karyopherin alpha 2 (RAG cohort 1, importin alpha 1), Karyopherin subunit alpha-2, pendulin, QIP2importin alpha 2, RAG cohort 1, RAG cohort protein 1, RCH1importin subunit alpha-2, SRP1, SRP1alpha, SRP1- ...
A nuclear localization signal or sequence (NLS) is an amino acid sequence that tags a protein for import into the cell nucleus by nuclear transport. Typically, this signal consists of one or more short sequences of positively charged lysines or arginines exposed on the protein surface. Different nuclear localized proteins may share the same NLS. An NLS has the opposite function of a nuclear export signal (NES), which targets proteins out of the nucleus. These types of NLSs can be further classified as either monopartite or bipartite. The major structural differences between the two is that the two basic amino acid clusters in bipartite NLSs are separated by a relatively short spacer sequence (hence bipartite - 2 parts), while monopartite NLSs are not. The first NLS to be discovered was the sequence PKKKRKV in the SV40 Large T-antigen (a monopartite NLS). The NLS of nucleoplasmin, KR[PAATKKAGQA]KKKK, is the prototype of the ubiquitous bipartite signal: two clusters of basic amino acids, ...
Wound healing is impaired in diabetes, resulting in significant morbidity and mortality. Neutrophils are the main leukocytes involved in the early phase of healing. As part of their anti-microbial defense, neutrophils form extracellular traps (NETs) by releasing decondensed chromatin lined with cytotoxic proteins. NETs, however, can also induce tissue damage. Here we show that neutrophils isolated from type 1 and type 2 diabetic humans and mice were primed to produce NETs (a process termed NETosis). Expression of peptidylarginine deiminase 4 (PAD4, encoded by Padi4 in mice), an enzyme important in chromatin decondensation, was elevated in neutrophils from individuals with diabetes. When subjected to excisional skin wounds, wild-type (WT) mice produced large quantities of NETs in wounds, but this was not observed in Padi4(-/-) mice. In diabetic mice, higher levels of citrullinated histone H3 (H3Cit, a NET marker) were found in their wounds than in normoglycemic mice and healing was delayed. Wound ...
MDRPLSSSAEAEEELEWQVASRRRKAWAKCRSSWQASETEDLSTEATTQDEEEDEEEDLPGAQLPAAGGR 1 - 70 GNVPNEKIAIWLKDCRTPLGASLDEQSSSTLKGVLVRNGGSFEDDLSLGAEANHLHESDAQIENCNNILA 71 - 140 KERRLQFHQKGRSMNSTGSGKSSGTVSSVSELLELYEEDPEEILYNLGFGRDEPDIASKIPSRFFNSSSF 141 - 210 AKGIDIKVFLSAQMQRMEVENPNYALTSRFRQIEVLTTVANAFSSLYSQVSGTPLQRIGSMSSVTSNKET 211 - 280 DPPPPLTRSNTANRLMKTLSKLNLCVDKTEKGESSSPSPSAEKGKILNVSVIEESGNKNDQKSQKIMKKK 281 - 350 ESSSMLATVKEEVSGSSAAVTENADSDRISDEANSNFNQGTENEQSKETQSHESKLGEESGIVESKLDSD 351 - 420 FNISSHSELENSSELKSVHISTPEKEPCAPLTIPSIRNIMTQQKDSFEMEEVQSTEGEAPHVPATYQLGL 421 - 490 TKSKRDHLLRTASQHSDSSGFAEDSTDCLSLNHLQVQESLQAMGSSADSCDSETTVTSLGEDLATPTAQD 491 - 560 QPYFNESEEESLVPLQKGLEKAAAVADKRKSGSQDFPQCNTIENTGTKQSTCSPGDHIIEITEVEEDLFP 561 - 630 AETVELLREASAESDVGKSSESEFTQYTTHHILKSLASIEAKCSDMSSENTTGPPSSMDRVNTALQRAQM 631 - 700 KVCSLSNQRMGRSLLKSKDLLKQRYLFAKAGYPLRRSQSLPTTLLSPVRVVSSVNVRLSPGKETRCSPPS 701 - 770 FTYKYTPEEEQELEKRVMEHDGQSLVKSTIFISPSSVKKEEAPQSEAPRVEECHHGRTPTCSRLAPPPMS 771 - 840 ...
Looking for online definition of karyopherin alpha 5 in the Medical Dictionary? karyopherin alpha 5 explanation free. What is karyopherin alpha 5? Meaning of karyopherin alpha 5 medical term. What does karyopherin alpha 5 mean?
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The putative regulatory role of the male reproductive hormones in the molecular mechanism underlying chromatin condensation remains poorly understood. In the past decade, we developed two adult male rat models wherein functional deficits of testosterone or FSH, produced after treatments with 20 mg/Kg/d of cyproterone acetate (CPA) per os, for a period of 15 days or 3 mg/Kg/d of fluphenazine decanoate (FD) subcutaneously, for a period of 60 days, respectively, affected the rate of sperm chromatin decondensation in vitro. These rat models have been used in the current study in order to delineate the putative roles of testosterone and FSH in the molecular mechanism underlying remodelling of sperm chromatin. We report that deficits of both testosterone and FSH affected the turnover of polyubiquitylated histones and led to their accumulation in the testis. Functional deficits of testosterone reduced expression of MIWI, the 5-methyl cap binding RNA-binding protein (PIWIlike murine homologue of the Drosophila
A short sequence of predominantly basic amino acids Pro-Pro-Lys-Lys-Lys-Arg-Lys-Val from SV40 Large T is responsible for the normal nuclear location of the protein. Alteration of Lys-128 to each of six different residues other than Arg renders Large T cytoplasmic, whereas single amino acid changes in the surrounding region impair but do not prevent nuclear accumulation. When transposed to the amino terminus of cytoplasmic Large T species, or Escherichia coli β-galactosidase or of chicken muscle pyruvate kinase, the sequence around Lys-128 of Large T is able to direct the recipient protein to the nucleus. This demonstrates that these amino acids can be sufficient for nuclear location and can act as a nuclear location signal. A computer search of over 2500 proteins reveals that some other nuclear proteins (for example, BK virus Large T, SV40 VP2 and adenovirus 72kDa DNA binding protein) contain very similar basic tracts, but so too do some presumed non-nuclear proteins (for example, poliovirus ...
DNA dsb represent a severe type of damage that can be provoked in cell nuclei by exposure to ionizing radiation and also to chemicals such as etoposide, methylmethane sulphonate and bleomycin. Both radiation and chemical agents induce a variety of other DNA lesions such as base and sugar modifications, and single-strand breaks mostly mediated by the formation of highly reactive oxygen radicals (Iliakis, 1991). Although dsb are perhaps the lesions that most strongly threaten cell survival, it is likely that damaged cells seek to repair all DNA lesions produced by the oxygen radicals, thus activating several DNA repair pathways (Wood, 1996). Therefore, by using these DNA damaging agents, it might be difficult to analyze exclusively the repair of DNA dsb. On the other hand, it is desirable to have an in vitro system to study the repair of dsb produced in an environment as close as possible to the cell nucleus.. We used prokaryotic restriction enzymes (REs) in order to introduce solely dsb in the ...
Looking for online definition of Sperm-specific protein in the Medical Dictionary? Sperm-specific protein explanation free. What is Sperm-specific protein? Meaning of Sperm-specific protein medical term. What does Sperm-specific protein mean?
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Despite the brain being the center of all bodily activity, it doesnt work on its own. Your brain uses your spinal cord to transport signals to all parts of the body. Since the spinal cord passes through our necks, that area is pretty vulnerable. If you feel persistent stiffness in your neck and back without any muscle strain, that may be a sign that a brain condition is affecting the spinal cord too. If you ever feel stiffness for unknown reasons, its best to consult a specialist because something harmless can have fatal consequences if improperly treated. ...
KPNA3 antibody, C-term (karyopherin alpha 3 (importin alpha 4)) for ICC/IF, IHC-P, IP, WB. Anti-KPNA3 pAb (GTX26038) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Halocynthia roretzi Hgv2 protein: amino acid sequence given in first source; sequence given for protein from H. roretzi; a putative histone-binding protein; homologous to Xenopus N1 protein; GenBank D13541
Karyopherin (importin) beta 3 RNAi available through Novus Biologicals. Browse our Karyopherin (importin) beta 3 RNAi catalog backed by our Guarantee+.
NPM1 - NPM1 (untagged)-Human nucleophosmin (nucleolar phosphoprotein B23, numatrin) (NPM1), transcript variant 3 available for purchase from OriGene - Your Gene Company.
Find out how well NLP could work for you. There are no right or wrong answers; please choose the single option from each question that is most true for you right now: ...
ヤギ・ポリクローナル抗体 ab31319 交差種: Hu 適用: WB,ELISA…Nucleophosmin抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
A nucleofosfomina (fosfoproteína nucleolar B23, numatrina ou NPM1) é uma proteína nucleolar ubiquamente expressada que transloca-se entre o núcleo celular e o citoplasma. Está implicada em múltiplas funções celulares, incluindo codificação proteica ribossomal e transporte, controle da duplicação do centrossomo e regulação do gene supressor de tumor ARF. ...