TY - JOUR. T1 - Argyrophilic nucleolar organizer regions (AgNORs) in mucosal epithelium under experimental denture bases in rats. AU - Hara, Tetsuya. AU - Sato, Takashi. AU - Mori, Shingo. AU - Shiral, Hajime. AU - Maruo, Yukinori. AU - Minagi, Shogo. PY - 2000/1. Y1 - 2000/1. N2 - The purpose of this study was to evaluate changes in the argyrophilic nucleolar organizer region (AgNOR) counts in mucosal epithelium induced by continuous or intermittent compressive pressure exerted through experimental denture bases and to examine the relationships between the AgNOR count, histopathological changes and the intensity of the pressure under denture bases. Continuous or intermittent compressive pressure exerted through the denture bases was applied to the hard palate of the molar region in rats. A morphometric analyses of AgNORs was performed in denture-supporting tissue 3 days and 1, 2, 4, 8, 12 and 20 weeks after the denture insertion. From the results of this study, it was found that non-pressure ...

TY - JOUR. T1 - Standardized AgNOR analysis as a prognostic parameter in endometrial carcinoma, endometrioid type. AU - Giuffrè, G.. AU - Fulcheri, E.. AU - Gualco, M.. AU - Fedele, F.. AU - Tuccari, G.. PY - 2001. Y1 - 2001. N2 - OBJECTIVE: To evaluate interobserver reproducibility of histologic grade in endometrial adenocarcinomas of endometrioid type (EC), to assess the relationships between nuclear grade and the amount of argyrophilic nucleolar organizer region (AgNOR) proteins and to determine the prognostic value of AgNOR proteins and the main clinicopathologic parameters. STUDY DESIGN: Architectural and nuclear grading were independently assessed by two pathologists in 64 formalin-fixed, paraffin-embedded surgical samples of EC obtained from an equal number of patients (age range, 38-84 years; mean, 63.5). Interobserver agreement was determined using the κ statistic; discrepant cases were reviewed, and a consensus was reached. Standardized AgNOR analysis was performed according to the ...

André A. Bell, Jens N. Kaftan, Til Aach, Dietrich Meyer-Ebrecht, Alfred Böcking: High Dynamic Range Images as a Basis for Detection of Argyrophilic Nucleolar Organizer Regions Under Varying Stain Intensities. ICIP 2006: 2541- ...

A Robertsonian translocation 45,XY, t(13q; 14q) was detected in the leukocyte cultures of a phenotypically normal male. Silver staining technique for nucleolus organizer regions revealed that both acrocentrics involved in the translocation had lost their nucleolus organizers.

Metaphase chromosome preparations were made from leukocyte cultures of normal individuals. The cells were fixed in methanol:acetic acid (3:1 v/v), then dropped on cold, wet slides which were air-dried before storage at 4 degrees C. The slides were st

1997. Departamento de Biologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brasil. Doctoral thesis. Orienting Professor: Dr. Yatiyo Yonenaga-Yassuda. Cytogenetic analysis of 133 specimens of Brazilian rodents of genus Akodon (Muridae: Sigmodontinae) from several localities of the State of São Paulo, Brazil, was performed in the present study. The analysis was carried out on the species A. cursor (2n = 14, 15 and 16), A. montensis (2n = 23, 24 and 25), A. nigrita (2n = 52) and A. serrensis (2n = 46). The metaphases obtained from bone marrow and fibroblast culture preparations were analyzed after GTG- and CBG-banding and nucleolus organizer regions after silver-staining (Ag-NORs) techniques. Some specimens were also analyzed by in situ hybridization (FISH) using telomeric and ribosomal probes, and by chromosome painting using probes obtained from microdissected chromosomes amplified by DOP-PCR (degenerated oligonucleotide priming-polymerase chain reaction). Twenty-four ...

DNA-directed RNA polymerase I subunit RPA34 is an enzyme that in humans is encoded by the CD3EAP gene. CD3EAP has been shown to interact with T-cell surface glycoprotein CD3 epsilon chain, POLR1E and POLR1C. GRCh38: Ensembl release 89: ENSG00000117877 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000047649 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Yamazaki T, Hamano Y, Tashiro H, Itoh K, Nakano H, Miyatake S, Saito T (Jul 1999). CAST, a novel CD3epsilon-binding protein transducing activation signal for interleukin-2 production in T cells. J Biol Chem. 274 (26): 18173-80. doi:10.1074/jbc.274.26.18173. PMID 10373416. Whitehead CM, Winkfein RJ, Fritzler MJ, Rattner JB (Mar 1998). ASE-1: a novel protein of the fibrillar centres of the nucleolus and nucleolus organizer region of mitotic chromosomes. Chromosoma. 106 (8): 493-502. doi:10.1007/s004120050271. PMID 9426281. Yamamoto K, Yamamoto M, Hanada K, Nogi Y, Matsuyama T, Muramatsu M (Jun 2004). ...

The dynamics of genome modification that occurred from the initial hybridization event to the stabilization of allopolyploid species remains largely unexplored. Here, we studied inheritance and expression of rDNA loci in the initial generations of Brassica napus allotetraploids (2n = 38, AACC) resynthesized from Brassica oleracea (2n = 18, CC) and B. rapa (2n = 20, AA) and compared the patterns to natural forms. Starting already from F1 generation, there was a strong uniparental silencing of B. oleracea genes. The epigenetic reprogramming was accompanied with immediate condensation of C-genome nucleolar organizer region (NOR) and progressive transgeneration hypermethylation of polymerase I promoters, mainly at CG sites. No such changes were observed in the A-genome NORs. Locus loss and gains affecting mainly non-NOR loci after the first allotetraploid meiosis did not influence established functional status of NORs. Collectively, epigenetic and genetic modifications in synthetic lines resemble ...

The standard rye cultivar Imperial and a structural variant carrying an intact 1R chromosome and two telocentric 1R chromosomes (short and long arms) were used to investigate expression patterns of homologous rDNA loci, and the influence of chromosome structural change on their interphase organisation and relative disposition. Sequential silver staining and in situ hybridization with the rDNA probe pTa71, established a correspondence between the expression and organization patterns of rDNA domains in metaphase and interphase cells. In most cells of the cultivar Imperial, nucleolar organizer region (NOR) silver staining on metaphase chromosomes with equivalent numbers of rDNA genes revealed a size heteromorphism between homologousrDNA loci, resulting from their differential expression. NOR heteromorphism in the structural variant line was significantly reduced. The preferential activity of one NOR over its homologue was found to be random within cells and independent of parental origin. ...

Nucleolin is a ubiquitous multifunctional protein involved in preribosome assembly and associated with both nucleolar chromatin in interphase and nucleolar organizer regions on metaphasic chromosomes in mitosis. Extensive nucleolin phosphorylation by a casein kinase (CKII) occurs on serine in growing cells. Here we report that while CKII phosphorylation is achieved in interphase, threonine phosphorylation occurs during mitosis. We provide evidence that this type of in vivo phosphorylation involves a mammalian homolog of the cell cycle control Cdc2 kinase. In vitro M-phase H1 kinase from starfish oocytes phosphorylated threonines in a TPXK motif present nine times in the amino-terminal part of the protein. The same sites which matched the p34cdc2 consensus phosphorylation sequence were used in vivo during mitosis. We propose that successive Cdc2 and CKII phosphorylation could modulate nucleolin function in controlling cell cycle-dependent nucleolar function and organization. Our results, along ...

Method to detect to argentafin proteins associated to the nucleolar organizing region (NOR).. Through Silver impregnation, of proteins (100KD) associated to Nucleolar organizer region (NOR). ...

TY - JOUR. T1 - Effect of diabetes mellitus on AgNOR count in mucosal epithelium subjected to continuous pressure under denture base. AU - Shirai, Hajime. PY - 1996. Y1 - 1996. M3 - Article. VL - 40. SP - 194. EP - 194. JO - The Journal of the Japan Prosthodontic Society. JF - The Journal of the Japan Prosthodontic Society. IS - 96specialNo.. ER - ...

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

Min pig has high lean meat percentage. When it reaches to carcass weight of 60kg, 90kg and 120kg, the lean meat percentage is about 53.29%, 46.13%, 39.14%, and its fat percentage rate is about 24.14%, 35.88% and 45.38%, respectively.. Utilization style:. 1. Crossbreeding programmes: When Min pig is used as a dam line and mated with Large White, Landrace, Soviet White, Berkshire, Northeast Spotted and Harbin white sires, the average daily gain of crossbred offsprings were 560A1A2544A1A2499A1A2526 and 575g, respectively. when used as a sire line and mated to west-north black and blue, Habai, Landrace and XinJin Pig dams, the average daily gain of crossbred offsprings were 615A1A2642A1A2555 and 531g, respectively.. Used as a dam in a three breed terminal crossbreeding scheme, the average dairy gain of the three breed crossbred offspring was higher than that of two breed crossbred offspring.. Evaluation of the genetic diversity: Zhan et al (1989) studied the silver staining nucleolus organizer ...

The different cell types of the body are formed in the right place and at the right time in response to signals that are produced by special organiser regions of the embryo. These so-called morphogens act in a concentration-dependent manner to induce the formation of different cell types at different positions within developing tissues. One of the earliest interactions of this kind is mesoderm induction, which results in the formation of organs and cell types such as heart, muscle, kidney and bone. We use frog, zebrafish and mouse embryos to study mesoderm-inducing factors and to ask how cells respond to them. One aim is to understand how the signals exert long-range effects in the embryo, and how cells distinguish between different morphogen concentrations to activate different genes. We go on to explore how these different genes then participate in the genetic regulatory networks that result in the formation of specific cell types. The principles we define in the early embryo inform additional ...

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Buitrago-Suarez UA, Burr BM. 2007. Taxonomy of the catfish genus Pseudoplatystoma Bleeker (Siluriformes: Pimelodidae) with recognition of eight species. Zootaxa 1512: 1-38. Campos JL. 2010. O cultivo do pintado (Pseudoplatystoma corruscans, Spix; Agassiz, 1829), outras espécies do gênero Pseudoplatystoma e seus híbridos. In: Baldisserotto B, Gomes LC, editors. Espécies nativas para a piscicultura no Brasil. 1st ed. Santa Maria (RS): Universidade Federal de Santa Maria; p. 335-361. Cioffi MB de, Moreira-Filho O, Ráb P, Sember A, Molina WF, Bertollo LAC. 2018. Conventional cytogenetic approaches - useful and indispensable tools in discovering fish biodiversity. Curr Genet Med Rep. 6: 176-186. Feldberg E, Bertollo LAC. 1985. Nucleolar organizing regions in some species of Neotropical cichlid fish (Pisces, Perciformes). Caryologia. 38: 319-324. Foresti F, Toledo LA. 1981. Polymorphic nature of nucleolus organizer regions in fishes. Cytogenet Genome Res. 31: 137-144. Foresti F, Oliveira C, ...

The nature and localization of DNA contained in the fibrillar centres and the dense fibrillar component (the fibrillar complex) in the nucleoli, was studied in human LEP cells, Sertoli cells, spermatogonia A and in mitotic chromosomes of stimulated lymphocytes. A novel procedure for isolating the intact fibrillar complex from LEP cells was used; the complex contains DNA that hybridizes to secondary constrictions of mitotic chromosomes and to 28 S rDNA sequences, on Southern blots. Electron microscopic DNA-DNA in situ hybridization was performed, with (a) a probe prepared from DNA extracted from the fibrillar complex of LEP cells, (b) a probe for human total genomic DNA, and (c) a probe for the transcribed part of human rDNA. On the basis of the results obtained we conclude that the ribosomal RNA genes in human Sertoli cells and spermatogonia A are predominantly associated with the dense fibrillar component, including the border region between fibrillar centres and the dense fibrillar component. ...

Abstract In this paper, we describe a Ty3-gypsy retrotransposon from allotetraploid peanut (Arachis hypogaea) and its putative diploid ancestors Arachis duranensis (A-genome) and Arachis ipaënsis (Bgenome). The consensus sequence is 11,223 bp. The element, named FIDEL (Fairly long Inter-Dispersed Euchromatic LTR retrotransposon), is more frequent in the A- than in the B-genome, with copy numbers of about 3,000 (±950, A. duranensis), 820 (±480, A. ipaënsis), and 3,900 (±1,500, A. hypogaea) per haploid genome. Phylogenetic analysis of reverse transcriptase sequences showed distinct evolution of FIDEL in the ancestor species. Fluorescent in situ hybridization revealed disperse distribution in euchromatin and absence from centromeres, telomeric regions, and the nucleolar organizer region. Using paired sequences from bacterial artificial chromosomes, we showed that elements appear less likely to insert near conserved ancestral genes than near the fast evolving disease resistance gene homologs. ...

How is Spindle-Cell Argyrophilic Mucin-Producing Carcinoma (oncology) abbreviated? SCAMPC stands for Spindle-Cell Argyrophilic Mucin-Producing Carcinoma (oncology). SCAMPC is defined as Spindle-Cell Argyrophilic Mucin-Producing Carcinoma (oncology) very rarely.

In ATT, a human autoimmune serum, we found anti-nucleolar antibodies that recognized nucleolar antigens confined to a single nucleolar compartment, the dense fibrillar component (DFC). We localized these antigens by immunoelectron microscopy in DFC of HeLa cell nucleoli both on Lowicryl sections and cryoultrathin sections without embedding. The antigens were solubilized by incubation with 2M NaCl but not by RNase or DNase treatment. The ATT serum crossreacted with rat liver nucleoli and PtK1 cell nucleoli in which immunofluorescence labelling displayed a clumpy pattern. During mitosis, the antigens dispersed in the cytoplasm until late telophase, when they gathered in the prenucleolar bodies. In human peripheral lymphocytes, or HeLa cells treated with actinomycin D, the antigens were still present but the fluorescence intensity decreased. By immunoblotting using human nuclear extracts, the ATT serum bound to a 116,000 Mr protein at dilutions up to 1:2000. The reactivity of this band diminished ...

The midbrain and cerebellum develop from an organizing center that is formed at the junction between the embryonic midbrain and hindbrain, known as the isthmus. This isthmic organizer (IsO) was discovered because of its property of inducing an ectopic midbrain or cerebellum, when transplanted into the chick diencephalon or hindbrain, respectively (reviewed by Liu and Joyner, 2001a; Wurst and Bally-Cuif, 2001). The IsO activity recruits the surrounding tissue into either a midbrain or cerebellum fate by controlling cell survival, proliferation and differentiation along the anteroposterior axis of the mid-hindbrain region. The formation of the IsO is the result of complex cross-regulatory interactions between transcription factors (Otx, Gbx, Pax and En) and secreted proteins (Wnts and Fgfs), culminating in the expression of the signaling molecule Fgf8 at the mid-hindbrain boundary (Liu and Joyner, 2001a; Wurst and Bally-Cuif, 2001; Ye et al., 2001). Fgf8 is the central mediator of IsO activity, as ...

In the interphase cell nucleus, the ribosomal genes are located in the fibrillar centers and in the associated dense fibrillar component of the nucleolus (Hernandez-Verdun, 1983; 1986; Goessens,...

Ken is on a business trip attending a conference in Seattle. He uses a personal organizer for planning his trips: the organizer is notified about changes affecting his itinerary (told by the airline, bus, or railway companies) and has rules to automatically react appropriately. While packing, Kens organizer receives a notification stating that his fight back home is canceled due to rain and he is instead booked for the next day. Fortunately Kens organizer has (reactive) rules in place to react to this event. Kens organizer automatically tries to extend Kens stay in the hotel. Since for the next night no single rooms are available, Ken is being placed in a more expensive double room. Before the booking is made, the new price is checked against the rules for business trip expenses of Kens company. Further the organizer recognizes that Kens coming home one day late affects its business and private plans. The organizer requests rescheduling of Kens business meetings on the next day by ...

organizer ( Page 163 ) 제조업체 주소록 - EC21에는 세계곳곳에서 등록한 3,000,000개의 organizer 수입업체, 수출업체, 제조업체, 공급업체, 도매업체, 유통업체, 무역회사, 셀러 등이 있습니다. EC21을 통해 쉽게 거래선을 발굴 하세요.

Location of the nucleolar ribosomal chromatin at telophase: as the nucleolus becomes active the ribosomal chromatin and associated ribonucleoprotein transcripts compose the more peripherally located dense fibrillar component

We often have one person booking a meeting or series of meetings (say training classes) that ends up being run by others. Would be nice if the meeting organizer could add another organizer or change the organizer to someone else. Currently only the organizer can take attendance. In our case, the organizer may not even be planning on attending the meeting.. ...

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RFC 5546 iTIP December 2009 The Delegator may continue to receive updates to the event even though they will not be attending. This is accomplished by the Delegator setting their role attribute to NON-PARTICIPANT in the REPLY to the Organizer. 3.2.2.4. Changing the Organizer The situation may arise where the Organizer of a VEVENT is no longer able to perform the Organizer role and abdicates without passing on the Organizer role to someone else. When this occurs, the Attendees of the VEVENT may use out-of-band mechanisms to communicate the situation and agree upon a new Organizer. The new Organizer should then send out a new REQUEST with a modified version of the VEVENT in which the SEQUENCE number has been incremented and the ORGANIZER property has been changed to the new Organizer. 3.2.2.5. Sending on Behalf of the Organizer There are a number of scenarios that support the need for a Calendar User to act on behalf of the Organizer without explicit role ...

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Ewelina Kurtys (Chair), André Lorenzoni (Organiser), Rieza Aprianto (Organiser), Harmen Booij (Organiser), Emilia Bigaeva (Member of programme committee), Ajinkya Patil (Organiser), Simon Hemelaar (Organiser), Wieke Holtkamp (Organiser), Bart Noort (Organiser), Alberto Llacua (Organiser), Andrea Soto Padilla (Member of programme committee), Jasmine Tomar (Member of programme committee), Floor Velthuis (Member of programme committee), Antonija Marjanovic (Member of programme committee), Marieke Adema (Member of programme committee ...

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Immunfluorescenciás eljárással kimutattuk, hogy a foszfatidilinozitol 4-kináz PI4K230 izoformája, más izoformáktól eltérően, patkányagy neuronális sejtjeiben és különféle neurális és nem neurális sejtvonalakban a citoplazmán kívűl a nukleoluszban is előfordul, ahol a dense fibrillar component szerkezetének kialakitásában és funkcióiban játszhat szerepet. A PI4K230 immunreaktivitás a nukleolusz degradációját okozó egyes antitumor ágensek hatására a sejtmagon belül diszpergálódik, ami alkalmassá teheti ezen ágensek hatékonyságának tesztelésére. A PI4K230 nukleáris transzportjában egy monopartite, nukleoláris lokalizációjában egy bipartite nukleáris lokalizációs szignál (NLS) szerepét valószínűsítettük. Maleimidek a PI4K230-at méretüktől függő módon a cAMP-függő proteinkinázhoz hasonlóan inaktiválják, feltehetően az ATP-kötődés sztérikus gátlását okozva és alátámasztva a két enzim katalitikus centrumának ...

The event organizers are particular acceptably in that and are wonderful specialists. In any case, there are a relentlessly making number of events of various bosses, for instance, stamp managers or driving profiles who in like course need to complete this movement of planning events. Both of the two cases can be a stunning and convoluted errand in the event that you dont have the vital push, contraptions and learning. Subsequently, we have created the post that will make your life less requesting in the event that you have to regulate events.. We have the keys to make the perceiving soundness less requesting and speedier, in light of the course by which that you dont relate all to a specific event and you can not duplicate yourself, isnt that so? How have you can achieved it? We let you know If you are an event organizer or a specialist who finishes it, you know flawlessly the rationalities and how to do it, paying little identity to at any given moment one of these stages may end up being ...

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Join Jan Kabili for an in-depth discussion in this video Importing the exercise files into Elements Organizer, part of Lightroom and Photoshop Elements: Integration

I use this organizer when teaching my students the anatomy of the heart. Its an interactive notes sheet that they can use to record information about atria, ventricles, valves and vessels. Answer key is included.

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Well list below 10 types of graphic organizers you can use during various scenarios, whatever youre reading, writing, doing research,or studying for exams.

The Department is the rights holder for the TT Races and as part of the licence granted by the Auto Cycle Union Limited (ACU) the Department is looking to appoint an organiser for a two year period to cover the TT Races for 2015 and 2016 with a potential extension of up to three years to include 2017, 2018 and 2019 Races ...

Milwaukee® PACKOUT™ Tool Organizer, Low Profile, 19.76 in L Size, 16.38 in Overall Width, 19.76 in Overall Depth, 2.52 in Overall Height, Polymer, ...

Organisers of AJ Tracey gig fined £10,000 for breaching Covid rulesRapper said he didnt expect that many people after huge crowds gathered in a Manchester park to see him perform AJ Tracey in 2019. Photograph: Joe Maher/Getty Images

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I am getting the following error when I Export to PDF from the IPLOT Main dialog or InterPlot Organizer:. Could not establish message connectivity with the Document Set Renderer (HRESULT=0x80004002). ...

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Parade organizers claimed to be in negotiations with TripAdvisor and other prospective corporate sponsors. TripAdvisor responded with a cease-and-desist letter, filled with titles of LGBTQ+ anthems.

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Leading up to the GC&E Conference we will be posting interviews with our 2017 GC&E Conference organizers to learn a little more about them and the

Organizers of the 2020 Tokyo Paralympics on Tuesday marked six months until the opening of the games. Athletes and officials commemorated the milestone wit

What is a Nucleolus Function? Nucleolus Function: The nucleolus is a round body located inside the nucleus of a eukaryotic cell. It is not surrounded by a… ...

What is a Nucleolus Function? Nucleolus Function: The nucleolus is a round body located inside the nucleus of a eukaryotic cell. It is not surrounded by a… ...

Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.

The nucleolus is a cellular structure found in the nucleus of eukaryotic cells. Some cells have several nucleoli, which work as a...

options to see if you can make it fit your requirements.. If the widget doesnt allow filtering by event category, Id consider a more complex (but also more flexible) system based on Event Organiser and CiviCRM Event Organiser which will definitely do what youre after. Have a look at the standard calendar, an event category and the list of events at a venue for the kind of things that are available out of the box.. ...