CILIARY NEUROTROPHIC FACTOR VARIANTS - Nucleic acid molecule selected from the group consisting of (a) a nucleic acid molecule having a nucleotide sequence shown in SEQ ID: NO 1, (b) a nucleic acid molecule which encodes a peptide having an amino acid sequence shown in SEQ ID: NO 2, (c) a nucleic acid molecule whose complementary strand hybridizes to a nucleic acid molecule according to (a) or (b) and which codes for a peptide which binds to ciliary neurotrophic factor receptor (CNTFR), the peptide binding with lower affinity than ciliary neurotrophic factor to the interleukin-6 receptor (IL-6R), (d) a nucleic acid molecule whose nucleotide sequence differs from the nucleotide sequence of a nucleic acid molecule according to (c) due to the degenerated genetic code, the codon at positions 82-84 of the nucleic acid molecule according to (a) coding for a non-positively charged amino acid, and the peptide at position 28 shown in SEQ ID: NO 2 having a non-positively charged amino acid residue ...
The invention features a method of identifying a nucleic acid molecule capable of post-transcriptional gene silencing by (a) affixing a plurality nucleic acid molecules onto a surface in discrete, defined locations; (b) contacting eukaryotic cells with the affixed nucleic acid molecules under appropriate conditions for entry of the nucleic acid molecules into the cells, whereby said nucleic acid molecules are introduced into the cells in the location in which they were affixed; and (c) determining the ability of the nucleic acid molecules to post-transcriptionally silence expression of a gene in the cells, wherein post-transcriptional gene silencing at a discrete, defined location identifies the nucleic acid molecules affixed at that location as being capable of post-transcriptional gene silencing.
Previously, we have demonstrated that the IFN-I signaling molecules, IRF9 and STAT1, were required for the production of IgG autoantibodies in the pristane model and for the high expression levels of TLR7 and TLR9 following treatment with IFN-I in B cells [32]. Here, we describe the autoantibody profile and TLR-dependent B-cell response in SLE mice genetically deficient in the IFNAR2 chain of the IFNAR. Autoantibody profiling using autoantigen microarrays in combination with conventional techniques to confirm the array results revealed that, similar to the phenotype for IRF9-/- mice, pristane-treated IFNAR2-/- mice specifically lacked IgG autoantibodies directed against all of the major targets in the pristane model. These targets included components of the RNA-associated complexes Sm/RNP and RiboP as well as the DNA-associated autoantigens ssDNA and histones. B cells from IFNAR2-/- mice exhibited defects in the expression of TLR7 as well as in responses to TLR7 agonists in the absence of ...
294547663 - EP 0985148 A1 2000-03-15 - NUCLEIC ACID DIAGNOSTICS BASED ON MASS SPECTROMETRY OR MASS SEPARATION AND BASE SPECIFIC CLEAVAGE - [origin: WO9854571A1] A method of detecting a mutation or a difference of one or more nucleotides between a nucleic acid molecule to be tested and a reference nucleic acid molecule, said method comprising subjecting the test nucleic acid molecule to base specific cleavage to generate oligonucleotide fragments, separating the resulting oligonucleotide fragments based on mass by MALDI-ATOF MS and/or other equivalent procedure to produce a fingerprint of then oligonucleotide fragments comprising one or more peaks wherein a peak represents the mass of each fragment and identifying an altered peak relative to a reference nucleic acid molecule subjected to the same procedure wherein the presence of an altered peak is indicative of a difference of one or more nucleotides in said tested nucleic acid molecule.[origin: WO9854571A1] A method of detecting a mutation or a
0021] Accordingly, the present invention concerns the preparation and use of miRNA arrays or miRNA probe arrays, which are macroarrays or microarrays of nucleic acid molecules (probes) that are fully or nearly complementary or identical to a plurality of miRNA molecules positioned on a support or support material in a spatially separated organization. Macroarrays are typically sheets of nitrocellulose or nylon on which probes have been spotted. Microarrays position the nucleic acid probes more densely such that up to 10,000 nucleic acid molecules can be fit into a region typically 1 to 4 square centimetres. Microarrays can be manufactured by spotting nucleic acid molecules, e.g., genes, oligonucleotides, etc., onto substrates or synthesizing oligonucleotide sequences in situ on a substrate. Spotted or in situ synthesized nucleic acid molecules can be applied in a high density matrix pattern of up to about 30 non-identical nucleic acid molecules per square centimetre or higher, e.g. up to about ...
294629575 - EP 1086212 A2 2001-03-28 - METHOD AND REAGENTS FOR THE TREATMENT OF DISEASES OR CONDITIONS RELATED TO MOLECULES INVOLVED IN ANGIOGENIC RESPONSES - [origin: WO9950403A2] Nucleic acid molecule which modulate the synthesis, expression and/or stability of an mRNA encoding for angiogenic factors selected from aryl hydrocarbon nuclear transport (ARNT), intergrin subunit beta 3 ( beta 3), integrin subunit alpha 6 ( alpha 6) and tie - 2 RNA. This invention further provides a treatment for indications related to angiogenesis using the nucleic acid molecules.[origin: WO9950403A2] Nucleic acid molecule which modulates the synthesis, expression and/or stability of an mRNA encoding for angiogenic factors selected from aryl hydrocarbon nuclear transport (ARNT), intergrin subunit beta 3 ( beta 3), integrin subunit alpha 6 ( alpha 6) and tie - 2RNA. This invention further provides a treatment for indications related to angiogenesis using the nucleic acid molecules.[origin: WO9950403A2] Nucleic acid molecule
The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site. A plurality of labelled types of nucleotide analogs are provided proximate to the active site, with each distinguishable type of nucleotide analog being complementary to a different
The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site. A plurality of labelled types of nucleotide analogs are provided proximate to the active site, with each distinguishable type of nucleotide analog being complementary to a different
The present invention includes polynucleotides encoding an MCP1 (e.g., a mature MCP1 polypeptide), an MCP1 multimer or a fusion thereof (e.g., fused to an in vivo half-life extending moiety (e.g., Ig)) (e.g., any of SEQ ID NOs: 1, 2, 8-12) as well as nucleic acids which hybridize to the polynucleotides. Preferably, the nucleic acids hybridize under low stringency conditions, more preferably under moderate stringency conditions and most preferably under high stringency conditions. A nucleic acid molecule is hybridizable to another nucleic acid molecule, such as a cDNA, genomic DNA, or RNA, when a single stranded form of the nucleic acid molecule can anneal to the other nucleic acid molecule under the appropriate conditions of temperature and solution ionic strength (see Sambrook, et a/., supra). The conditions of temperature and ionic strength determine the stringency of the hybridization. Typical low stringency hybridization conditions are 55°C, 5X SSC, 0.1% SDS, 0.25% milk, and no ...
www.MOLUNA.de Protocols for Nucleic Acid Analysis by Nonradioactive Probes [4221542] - Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots,
The present invention relates, e.g., to a method for detecting a nucleic acid molecule of interest in a sample comprising cell-free nucleic acids, comprising fluorescently labeling the nucleic acid molecule of interest, by specifically binding a fluorescently labeled nanosensor or probe to the nucleic acid of interest, or by enzymatically incorporating a fluorescent probe or dye into the nucleic acid of interest, illuminating the fluorescently labeled nucleic acid molecule, causing it to emit fluorescent light, and measuring the level of fluorescence by single molecule spectroscopy, wherein the detection of a fluorescent signal is indicative of the presence of the nucleic acid of interest in the sample.
The invention relates to an isolated nucleic acid molecule encoding a caspase-14 polypeptide or functional fragment thereof, a vector that contains the nucleic acid molecule and a host cell that contains the vector. The invention also relates to an isolated gene encoding caspase-14, as well as functional fragments thereof. The gene or nucleic acid molecule can include single or double stranded nucleic acids corresponding to coding or non-coding strands of the caspase-14 nucleotide sequence. Isolated caspase-14 polypeptides or functional fragments thereof are also provided, as are antibodies that specifically bind thereto. In addition, the invention relates to methods of identifying compounds that modulate caspase-14 activity.
Synthetic nucleic acids are produced routinely for a wide variety of applications, including biological and chemical research, and diagnostic or therapeutic applications
The invention relates to methods for the detection of the amount of a nucleic acid in a sample. The described methods exploit the ability to physically pair nucleic acid molecules in a sample that have a reference sequence with nucleic acid molecules in the sample that have a target sequence. The presence of unpaired target or reference sequence following such physical pairing indicates a difference in the amount of the target sequence versus the reference sequence. The methods are broadly applicable for the determination of differences in the amounts of nucleic acids in diagnostic and research applications.
The invention provides a rapid, sensitive and specific nucleic acid detection system which utilizes isothermal nucleic acid amplification in combination with a lateral flow chromatographic device, or DNA dipstick, for DNA-hybridization detection. The system of the invention requires no complex instrumentation or electronic hardware, and provides a low cost nucleic acid detection system suitable for highly sensitive pathogen detection. Hybridization to single-stranded DNA amplification products using the system of the invention provides a sensitive and specific means by which assays can be multiplexed for the detection of multiple target sequences.
May 13, 2019 · Cytomegalovirus, or CMV, is a common cause of disease in the transplant population. , high cytomegalovirus nucleic acid detection so there is a high seroprevalence. Because it is a member of the Herpesviridae family, , high cytomegalovirus nucleic acid detection molecular detection of CMV nucleic acid in clinical specimens (e.g., using real-time PCR) has become a common approach. In addition to qualitative detection of the , high cytomegalovirus nucleic acid ...
Initially, analytical instrumentation, including a dual-force aggregation platform as well as a microdevice for integrated PCR amplification and HIA detection, were engineered to maximize the utility of bead aggregation assays in terms of throughput, speed, and sensitivity. In subsequent work, the HIA technique was exploited for the detection of single nucleotide polymorphisms. This was demonstrated for the detection of KRAS mutations in lung and colorectal cancers in order to predict patient sensitivity to epidermal growth factor receptor-targeted therapies. Additionally, HIA detection was combined with multiplex allele-specific PCR to detect three important mutations [CYP2C9 *2, CYP2C9 *3, and VKORC1 (1173C,T)] that allow appropriate dosing of the common oral anticoagulant, warfarin. Overall, this work represents practical steps forward in the development of nucleic acid analysis technology that is amenable for routine clinical care, in order to ultimately reap the rewards of a personalized ...
Nanogen, Inc., developer of advanced diagnostic products, announced today its subsidiary, Epoch Biosciences, was issued Patent No. 6,951,930, Hybridization-Triggered Fluorescent Detection of Nucleic Acids by the U.S. Patent and Trademark Office. The 930 patent relates to latent fluorophore-minor groove binder oligonucleotide conjugates which fluoresce upon hybridization to a target. The conjugates may be used to detect nucleic acid targets. Source: ...
MOLECULAR STRUCTURE AND SPECTRA OF MACROMOLECULES: CYCLIC-TETRAPYRROLE COMPOUNDS AND THEIR METAL COMPLEXES, NUCLEIC-ACID BASES AND THEIR METHYLATED SPECIES.
Early stromal edema can often be detected biomicroscopicallv by noticing fine, undulating striae in the deep stroma and Descemets membrane (deep striate keratopathy, auti sm DSK), caused as the cornea expands posteriorly with thickening. Development of Polyion Complex Micelles from Peppcid for the Delivery of Pepcid ac autism Acid-Based Therapeutics An exciting area of research involving micelles is the efforts to deliver nucleic acid-based therapeutics pepcid ac autism. J. For example, suppose the candidateвs apparent proportion of support was 53 rather than 60.
The present invention relates to use of a nucleic acid molecule having intra-molecular base pairing as an internal control in nucleic acid amplification. The invention relates to a method of nucleic acid amplification comprising having components which amplify target nucleic acid if it is present in a sample and comprising a nucleic acid molecule having intra-molecular base pairing as an internal control. The invention also relates to a kit for use in the method.
6,132,718 Multi-stage cascade boosting vaccine 6,130,364 Production of antibodies using Cre-mediated site-specific recombination 6,130,316 Fusion proteins of novel CTLA4/CD28 ligands and uses therefore 6,127,598 NKX-2.2 and NKX-6.1 transgenic mouse models for diabetes, depression, and obesity 6,121,415 ErbB4 receptor-specific neuregolin related ligands and uses therefor 6,121,030 CSAPK-2 protein and uses therefor 6,118,045 Lysosomal proteins produced in the milk of transgenic animals 6,117,654 Nucleic acid molecules encoding Tango-77-polypeptides 6,117,650 Assay for cardiac hypertrophy 6,114,598 Generation of xenogeneic antibodies 6,114,123 Lipocalin family protein 6,111,092 Nucleic acid molecules encoding DRT111 and uses thereof 6,110,739 Method to produce novel embryonic cell populations 6,110,718 Mammalian putative phosphatidylinositol-4-phosphate-5-kinase 6,107,543 Culture of totipotent embryonic inner cells mass cells and production of bovine animals 6,107,472 Receptor-type tyrosine ...
Compositions, recombinant vaccines and live attenuated pathogens comprising one or more isolated nucleic acid molecules that encode an immunogen in combination with an isolated nucleic acid molecule that encodes IL-28 or a functional fragment thereof are disclosed. Methods of inducing an immune response in an individual against an immunogen, using such com-positions are disclosed.
An outgoing Material Transfer Agreement (MTA) is required in some instances and recommended in others. For assistance, refer to the Office of Technology Development (OTD) website.
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Vertical and seasonal variations of bacterioplankton subgroups with different nucleic acid contents: Possible regulation by phosphorus
Protein-nucleic acid interaction is an important process in many biological phenomena. In this study, a fluorescence resonance energy transfer (FRET)-based protein-DNA binding assay has been developed
The structural biology of protein-nucleic acid interactions is in some ways a mature field and in others in its infancy. High-resolution structures of protein-DNA complexes have been studied since the mid 1980s and a vast array of such structures has now been determined, but surprising and novel structures still appear quite frequently. High-resolution structures of protein-RNA complexes were relatively rare until the last decade. Propelled by advances in technology as well as the realization of RNAs importance to biology, the number of example structures has ballooned in recent years. New insights are now being gained from comparative studies only recently made possible due to the size of the database, as well as from careful biochemical and biophysical studies. As a result of the explosion of research in this area, it is no longer possible to write a comprehensive review. Instead, current review articles tend to focus on particular subtopics of interest. This makes it difficult for newcomers to the
Despite the fact that non-viral nucleic acid delivery systems are generally considered to be less efficient than viral vectors, they have gained much interest in recent years due to their superior safety profile compared to their viral counterpart. Among these synthetic vectors are cationic polymers, branched dendrimers, cationic liposomes and cellpenetrating peptides (CPPs). The latter represent an assortment of fairly unrelated sequences essentially characterised by a high content of basic amino acids and a length of 10-30 residues. CPPs are capable of mediating the cellular uptake of hydrophilic macromolecules like peptides and nucleic acids (e.g. siRNAs, aptamers and antisenseoligonucleotides), which are internalised by cells at a very low rate when applied alone. Up to now, numerous sequences have been reported to show cell-penetrating properties and many of them have been used to successfully transport a variety of different cargos into mammalian cells. In recent years, it has become apparent that
article{744458, author = {Remaut, Katrien and Sanders, Niek and De Geest, Bruno and Braeckmans, Kevin and Demeester, Jo and De Smedt, Stefaan}, issn = {0927-796X}, journal = {MATERIALS SCIENCE \& ENGINEERING R-REPORTS}, keyword = {SINGLE-PARTICLE TRACKING,FLUORESCENCE CORRELATION SPECTROSCOPY,CROSS-CORRELATION SPECTROSCOPY,advanced light microscopy,nuclear uptake,endocytosis,extracellular matrix,non-viral carriers,gene therapy,GLYCOL-POLYETHYLENIMINE/DNA COMPLEXES,CYSTIC-FIBROSIS SPUTUM,BLOCK-COPOLYMER MICELLES,RESONANCE ENERGY-TRANSFER,CAVEOLAE-MEDIATED ENDOCYTOSIS,INTRAVENOUS GENE DELIVERY,IMAGE CORRELATION SPECTROSCOPY}, language = {eng}, pages = {117--161}, title = {Nucleic acid delivery: Where material sciences and bio-sciences meet}, url = {http://dx.doi.org/10.1016/j.mser.2007.06.001}, volume = {58}, year = {2007 ...
Isolation, amplification, and detection of DNA and RNA sequences in molecular diagnostic devices are at the forefront of modern diagnostic medicine. Such technologies offer unprecedented sensitivity and specificity in the detection of infectious disease. Molecular diagnostics are typically very expensive, large, and require a modern laboratory and trained technicians to operate---greatly restricting the use of molecular diagnostic tools in the developing world.. DFA is building paper-based technology to perform molecular diagnostics on an extremely inexpensive, disposable device. DFA is currently developing a nucleic acid amplification-based paper-microfluidic device for early infant diagnosis of HIV under a Saving Lives at Birth grant. Prior test kit development included a DARPA-funded effort to identify E. coli and a DTRA-funded effort with Harvard University focused on Brucella abortus. We believe our approach represents a fundamental shift in the field of nucleic acid detection that can ...
PrimeIAmp™ COVID-19 Lyophilized Fluorescence Nucleic Acid Detection Kit utilizes world-class isothermal amplification technology to detect COVID-19 nucleic acid by quantitative PCR
Surface Enhanced Raman as Tool to study Drug Protein Interactions and Nucleic Acid Detection. Chandrabhas Narayana (BHAS) Chemistry and Physics of Materials Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., Bangalore 560064, India [email protected] Slideshow 2716597 by raven
Beijing Wantai Biological item WS-1248 Beijing Wantai Biological WS-1248 Nucleic Acid Detection Kit COVID. Contact Sanbio for more information.
We are looking for global agents [Product Name] Monodon baculo virus disease(MBV)Nucleic acid Detection Kit(Freeze-dried/qPCR Method)V2.0 [Packaging Specification] 24 test/box [Storage condition and term of validity]Stored at room temperature in a...
Nucleic acid detection by pattern recognition receptors. Virus infection delivers nucleic acids into infected cells. (Left) DNA is detected in the cytoplasm by
Read independent reviews on Southern-Light™ and Southern-Star™ Chemiluminescence Nucleic Acid Detection System from Thermo Fisher Scientific on SelectScience
It is required under the NIH Guidelines that the IBC maintains a membership of a minimum of five individuals, with expertise covering knowledge of recombinant DNA and synthetic nucleic acid molecules, a plant expert, an animal expert, and at least two members that are from the community, not affiliated with UWM. Large-scale or BSL-3 research requires the additional membership of a Biological Safety Officer (BSO). At UWM, the biological safety officer always sits on the IBC and currently serves as the IBC coordinator. A current record of membership is reported annually to the NIH Office of Science Policy (OSP) by the biological safety officer.. In addition to evaluation of biological research, the IBC and Biological Safety Program provide guidance and support to the research community of UWM by evaluating best practices and developing guidance documents for research facilities to follow in their own research. The IBC reserves the right to determine the BSL for researchers work and to recommend ...
I certify that the information provided in my protocol submission form is accurate; and any protocol changes, including the DNA being cloned, the vector, the host organism, or any other toxic or infectious agents, will be submitted to the IBC for approval prior to initiation.. I further certify that I have read and will comply with all relevant publications, including but not limited to the NIU Institutional Biosafety Committee Policy, the CDC/NIH Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition and the Department of Health and Human Services National Institutes of Health Guidelines for Research Involving Recombinant DNA Molecules or Synthetic Nucleic Acid Molecules.. ...
Low-density lipoprotein receptor (LDL-R) is a cell surface receptor protein expressed in a variety of solid cancers including lung, colon, breast, brain and liver, therefore opens up opportunities to deliver lysosome sensitive anti-cancer agents, especially synthetic nucleic acid-based therapeutic mole- cules. In this study, we focused on developing novel nucleic acid molecules specific to LDL-R. For this purpose, we performed in vitro selection procedure via SELEX methodologies using mammalian cell- expressed human recombinant LDL-R protein as a target. After ten rounds of selections, we identified a novel DNA oligonucleotide aptamer, RNV-L7, that can bind specifically to LDL-R protein with high affinity and specificity (Kd = 19.6 nM). Furthermore, flow cytometry and fluorescence imaging assays demonstrated efficient binding to LDL-R over-expressed human cancer cells including Huh-7 liver can- cer cells and MDA-MB-231 breast cancer cells with a binding affinity of ∼200 nM. Furthermore, we ...
Microbial transcription modulator NusG interacts with RNA polymerase and termination factor rho, displaying striking functional homology to eukaryotic Spt5. The protein is also a translational regulator. We have determined crystal structures of Aquifex aeolicus NusG showing a modular design: an N-terminal RNP-like domain, a C-terminal element with a KOW sequence motif and a species-specific immunoglobulin-like fold. The structures reveal bona fide nucleic acid binding sites, and nucleic acid binding activities can be detected for NusG from three organisms and for the KOW element alone. A conserved KOW domain is defined as a new class of nucleic acid binding folds. This module is a close structural homolog of tudor protein-protein interaction motifs. Putative protein binding sites for the RNP and KOW domains can be deduced, which differ from the areas implicated in nucleic acid interactions. The results strongly argue that both protein and nucleic acid contacts are important for NusGs functions ...
In 1944, Oswald Avery, Colin MacLeod, and Maclyn McCarty published an article in which they concluded that genes, or molecules that dictate how organisms develop, are made of deoxyribonucleic acid, or DNA. The article is titled Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types: Induction of Transformation by a Desoxyribonucleic Acid Fraction Isolated from Pneumococcus Type III, hereafter Transformation. The authors isolated, purified, and characterized genes within bacteria and found evidence that those genes were made of DNA and not protein.. Format: Articles Subject: Publications ...
1. The addition of heparin to rat liver, kidney, or brain nuclei has been found to bring about the release of a gel. Chemical analysis and histochemical studies on whole homogenates and isolated nuclei demonstrated that the material released by heparin contained desoxyribonucleic acid (DNA) and protein. The action of heparin on nuclei is interpreted as the result of a combination with the basic proteins of the nucleus with a consequent displacement of DNA.. 2. The addition of heparin to a finely divided dilute liver homogenate prepared in a phosphate-sucrose solution at pH 7.1 brings about a marked increase in viscosity which reaches a maximum in 6 to 8 minutes at 23° and then declines.. 3. The concentration threshold for the viscosity effect was 0.1 mg. per 100 mg. fresh rat liver, with further increases in viscosity at higher heparin concentrations. Over a period of several hours a marked decrease in response to heparin was observed in homogenates stored at 0°.. 4. Fractionation of the ...
Synonyms for episomal DNA in Free Thesaurus. Antonyms for episomal DNA. 2 synonyms for DNA: deoxyribonucleic acid, desoxyribonucleic acid. What are synonyms for episomal DNA?
Heritable type-specific traits have been induced in meningococcus populations by exposure to desoxyribonucleic acid (DNA)-containing extracts derived from meningococcus cells of the type desired. The DNA has been shown to be an essential component of
Genetic Science is a new online OPEN-ACCESS journal opened in the frame of Symbiosis (Bloomington, Illinois, USA) to help gather the formidable knowledge provided for studies on genome, gene, desoxyribonucleic acid (DNA)….
Endomatrial crabmeatous neoplastic distemper Endometrium is the immanent liner of the mammal womb and actually(prenominal) sensitive ductless gland change, cross to menstrual cycle. endometrial malignant neoplastic un backbreakingness is a belated maturity date crabmeat delimitate as a delineate of which the booths of the endometrial line of womb obligate heighten irrepressible or occupy away malignant as a head of the emasculatenation of jail booths desoxyribonucleic acid. Its the quaternary closely reciprocal malignant neoplastic illness among women e very(prenominal)where every(prenominal) in all, later on rapper pubic lo hand instigateher mixture, lung malignant neoplastic disease, and in running gameine crabmeat.Ca recitations and happen accompanimentors 1. phase angle roughly endometrial crab lo substance ab subroutine occurs to women in mid(prenominal) cardinal and honest-to- substantiallyness. It washcloththor n be ca employ by very a great deal than sepa stoole(a) create from raw stuff ...
This study applied a case-control approach to investigate the association between low-grade inflammation, defined by high values within the normal range of C-reactive protein (CRP) and interleukin-6 (IL-6), and urinary markers of nucleic acid oxidation. No differences in excretion of urinary markers of nucleic acid oxidation between cases and controls were found and multivariable linear regression analysis showed no association between urinary markers of nucleic acid oxidation and inflammatory markers. Post-hoc multivariable linear regression analysis showed significant associations between nucleic acid oxidation and various iron status markers and especially a close relationship between nucleic acid oxidation and ferritin. This study shows no association between low-grade inflammation and urinary markers of nucleic acid oxidation in a population of elderly Italian people. The results suggest that low-grade inflammation only has a negligible impact on whole body nucleic acid oxidation, whereas ...
Try the new Google Patents, with machine-classified Google Wie Psoriasis qd behandeln results, and Japanese and South Korean patents. Kallikrein gen Conditions kallikrein translated from German DE T2. An isolated protein consisting of an amino acid sequence of SEQ ID NO: An isolated nucleic acid molecule of claim 2 with a nucleic acid sequence of SEQ ID NO: A vector comprising a nucleic acid molecule according to claim 2 or 3.. A host cell comprising wie Psoriasis qd behandeln nucleic acid molecule of claim 2 or 3. A process for producing a protein according to claim 1, which comprises: Antibodies having specificity Psoriasis Zimt an epitope of a protein of claim wie Psoriasis qd behandeln. The antibody of claim 7 which is wie Psoriasis qd behandeln with a detectable substance and used to detect the polypeptide in biological samples, tissues and cells.. Probe comprising a sequence that encodes a protein of claim 1. A test kit for diagnosing a condition associated wie Psoriasis qd behandeln a ...
Swedish University dissertations (essays) about NUCLEIC-ACID BASES. Search and download thousands of Swedish university dissertations. Full text. Free.
There has been some confusion among the names of dyes. Methyl green and light green are completely different in their uses. It would be possible to go on and on about this, but it would take up at least 4 screenfuls of text. All the information is there in the textbooks. Four points are, however, worth emphasizing. 1. Methyl green (CI 42585) was a lousy dye (always contaminated with crystal violet, which had to be extracted with chloroform) and it is no longer sold. Dyes sold as methyl green are really ethyl green (CI 42590). If the catalogue doesnt say this, dont buy the stuff - they might just be trying to pass off ancient stocks of real methyl green. If you look in a good catalogue (Sigma, Aldrich, and Im sure many others), the real identity of the dye will be clearly stated. Its up to us, the users, to start asking for ethyl green rather than methyl green. 2. Ethyl/methyl green is a cationic dye used for staining nuclei by virtue of their DNA content. Used alone, it also stains other ...
The amount of single stranded nucleic acid molecule reactants present in the aqueous reaction solution can range from an upward extreme in which the second single stranded nucleic acid molecule is present in an initial concentration such that at least a 100 fold increase in acceleration of rate of reaction is observed in the presence of 4M LiCl compared with the rate or reaction observed with 0.18 M NaCl at 60°C, to a lower extreme on the order of 10-9 micrograms. Interestingly, the reaction rate increase for high concentrations of DNA:DNA or DNA:RNA reactions is lower than that for low concentrations of DNA:DNA and RNA:RNA reactions. Thus, the method of the present invention is applicable to both high and low concentrations of reactants. Along these lines, preferred reaction solution volumes will be on the order of a millilitre or less to a fraction of a microlitre. However, it should be emphasized that other reaction solution volumes are contemplated as being within the scope of the present ...
Summary of Facts and Submissions. I. European patent No. 0 972 041 is based on European patent application No. 98 915 305.1, published as International patent application WO 98/45434 (hereinafter the application as filed), and was granted with 34 claims. Claims 1, 13 and 21 read as follows:. 1. An isolated nucleic acid molecule selected from the group consisting of:. a) a nucleic acid molecule comprising a nucleotide sequence which is at least 75% identical to the nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:3, the cDNA insert of the plasmid deposited with the ATCC as Accession Number 98348, or a complement thereof; .... 13. An isolated polypeptide selected from the group consisting of:. a) a polypeptide comprising an amino acid sequence which is at least 75% homologous to the amino acid sequence of SEQ ID N:2; .... 21. An antibody or antibody fragment that selectively binds to the polypeptide of claims 13, 17, 18 or 19.. Claim 1 contained paragraphs (b) to (g) defining further ...
Recently, nucleic acid secondary structures, G-quadruplexes in particular, have received much attention. G-quadruplexes are non-canonical nucleic acid secondary structures that are formed from G-rich sequences. These sequences consist of four stretches of G residues (each stretch with two or more G residues) interspersed by sequences of variable composition that form the loops. The formation of G-quadruplexes is induced and stabilized by monovalent cations like potassium and sodium. The presence of G-quadruplexes was first reported in telomeres and subsequently in the promoter region of several genes, 5′UTR (untranslated regions) and 3′UTRs [1-6]. G-quadruplexes function as regulatory elements and can influence key biological processes including transcription [3], translation [4] and splicing [7]. Recently, G-quadruplexes were also reported to be enriched at certain positions of eukaryotic retrotransposons, which correspond to regulatory regions of genes and viruses [8, 9]. Genome-wide ...
Metallic nanoparticles functionalized with oligonucleotides are used for a number of nucleic acid detection strategies. However, oligonucleotide-nanoparticle conjugates suffer from a lack of stability when exposed to certain conditions associated with DNA detection assays. In this study, we report the synthesis of thiol and thioctic acid-modified oligonucleotide gold nanoparticle (OGNs) conjugates functionalized with a dye label and varying spacer groups. The thioctic acid-modified conjugates exhibit increased stability when treated with dithiothreitol (DTT) compared to the more commonly used thiol modification. When the dye labelled oligonucleotide nanoparticle conjugates are exposed to the same conditions there is a pronounced increase in the stability for both thioctic acid and thiol modified sequences. These results open up the possibility of simply using a dye label to enhance the stability of oligonucleotide-nanoparticle conjugates in DNA detection assays where the enhanced stability of ...
MARKET MONITOR GLOBAL, INC (MMG) has surveyed the Human Mycoplasma Nucleic Acid Detection Kit manufacturers, suppliers, distributors and industry experts on this industry, involving the sales, revenue, demand, price change, product type, recent development and plan, industry trends, drivers, challenges, obstacles, and potential risks ...
In contrast to Risk Groups, Biosafety Levels (BSL) prescribe procedures and levels of containment for the particular microorganism or material (including Research Involving Recombinant or Synthetic Nucleic Acid Molecules). Similar to Risk Groups, BSL are graded from 1 - 4. Detailed descriptions of containment practices and biosafety levels can be found in the CDC-NIH Guidelines Biosafety in Microbiological and Biomedical Laboratories.. The majority of work at UVA involves Biosafety Level 2 (BSL-2) practices. BSL-2 containment and practice is suitable for work with agents that are infectious to humans or animals where exposure may result in limited to moderate disease. The routes of exposure to these agents are typically through cuts and breaks in the skin, ingestion, and splashes to the mucous membranes (eyes, nose, and mouth). These agents or materials include:. ...
Hi, Poly Scientific has a nice methyl green solution ready to use. I dont recall the cat #, sorry. The procedure is quite simple if you remember to keep water and alcohol out. 1 minute in the counterstain then three changes of acetone then three changes of xylene, then coverslip. Check the stain to be sure you dont need to tweak it. Sometimes you need 15- 20 seconds more or less time. piece o cake :-) Amos Brooks Tina Cardamone wrote: , Hello, , , I have a simple question regarding methyl green. , Does anyone have a methyl green counterstain protocol for , immunohistochemistry. , It sounds simple however I cant seem to find a protocol anywhere. , , Thanks in advance. , , Tina Cardamone , University of Melbourne ...
Refractive index increment dn/dc values when you need them? In light Equation displaying K=2 pi^2 n^2/lambda^4 / Since the refractive index is intuitively related to the density / specific volume of Starch, Aqueous Buffer, DNA = desoxyribonucleic acid ; RNA = ribonucleic acid ; SDS = sodium. measuring the refractive index which has the added advantage of requiring as little as 20 μl of sample. There is a simple linear relationship between refractive index and the density. (e.g. sucrose and NaCl). values for iodixanol solutions ) to get an absorbance . SDS-polyacrylamide and agarose gel electrophoresis. A Brix degree is a measure of the density or concentration of sugar solutions. There are two Relationship of Brix Tolerance to Refractive Index Tolerance. ...
Synonyms for Circular DNA in Free Thesaurus. Antonyms for Circular DNA. 2 synonyms for DNA: deoxyribonucleic acid, desoxyribonucleic acid. What are synonyms for Circular DNA?
Special precautions should be taken when working with pantropic and amphotropic vectors, which are capable of infecting human cells.. Integration and Mutagenesis Potential of Viral Vectors. The DNA of some viruses, such as lentivirus and gammaretrovirus, is able to integrate in the genome of the host cells as a provirus. Integration of the viral genome may disrupt endogenous genes resulting in mutations. This can lead to a wide range of disorders depending on the site of integration. Of special concern are the activation of proto-oncogenes and inactivation of tumor-suppressor genes, which can lead to the development of cancer.. Environmental Stability of Viral Vectors. Viruses may be more or less sensitive to external factors depending on the presence or absence of viral envelopes. Naked or non-enveloped viruses - e.g., adenovirus - exit the host cell by lysis. Enveloped viruses - e.g., retroviruses - exit the host cell by budding through the cell membrane. The envelope is composed of fragments ...
Sofinnova Partners announces DNA Script successful Series A fundraising of €11 M to advance development of its DNA synthesis technology. Paris, September 12th 2017. Sofinnova Partners, a leading European venture capital firm specialized in Life Sciences, today announced that portfolio company DNA Script, an industry leader in the manufacture of de novo synthetic nucleic acids using proprietary enzymatic technology, has raised €11 million. Sofinnova Partners is DNA Scripts historic and leading shareholder. Alongside existing shareholders which seeded the company, Sofinnova Partners, Kurma Partners, and Idinvest Partners, new high profile investors join the company for this Series A financing round: Illumina Ventures, and Merck Ventures BV (known as M Ventures in the United States and Canada), the corporate venture arm of Merck KGaA.. Founded in 2014 in Paris (France), DNA Script is the worlds leading company in the manufacturing of de novo synthetic nucleic acids using an enzymatic ...
Oligonucleotide probes are effective tools for the research of nucleic acids. This project discusses about Oligonucleotide Probes, Multiplex Genetic Analyses, Multiplex nucleic acid analyses, Genetic variation, Different Types of nucleic acid analyses, multiplex oligonucleotide design,
1. Nucleic-acid mediated immunity & Inflammation· Innate immune sensing pathways of foreign RNA/DNA · Innate immune sensing of endogenous retroviruses and inflamm...
Nucleic acid chaperone activity is an essential component of reverse transcription in retroviruses and retrotransposons. Using DNA stretching with optical tweezers, we have developed a method for detailed characterization of nucleic acid chaperone protein
Generic CHLORAMPHENICOL; DESOXYRIBONUCLEASE; FIBRINOLYSIN availability. Has a generic version of CHLORAMPHENICOL; DESOXYRIBONUCLEASE; FIBRINOLYSIN been approved? Find suppliers, manufacturers, and packagers
Twisted intercalating nucleic acid (TINA) is a nucleic acid molecule that, when added to triplex-forming oligonucleotides (TFOs), stabilize Hoogsteen triplex DNA formation from double-stranded DNA (dsDNA) and TFOs. Its ability to twist around a triple bond increases ease of intercalation within double stranded DNA in order to form triplex DNA. Certain configurations have been shown to stabilize Watson-Crick antiparallel duplex DNA. TINA-DNA primers have been shown to increase the specificity of binding in PCR. The use of TINA insertions in G-quadruplexes has also been shown to enhance anti-HIV-1 activity. TINA stabilized PT demonstrates improved sensitivity and specificity of DNA based clinical diagnostic assays. Triple helixes are formed when a single-stranded triplex-forming oligonucleotide (TFO) binds to a purine-containing strand of dsDNA through specific major groove interactions. Generally, the third-strand affinity of a TFO is low, due to the requirement for the formation of pH-sensitive ...
Electrostatic forces and potentials are keys in determining the interactions between biomolecules. We have recently imaged the topography and electrostatic surface potential of nucleic acid molecules on silicon surfaces using Kelvin probe force microscopy (KPFM). Here, we demonstrate KPFM imaging on insulating surfaces like mica, which provides access to configurations of DNA that are projections of its structure in solution. In particular, we apply dual-frequency mode to minimize the tip-sample distance at which the Kelvin probe signal is acquired and use the fundamental resonance of the cantilever to determine surface potential and its first overtone to detect the topography. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3512867 ...
Magnetic separation technology To ease and accelerate nucleic acid isolation based on magnetic particles, and to offer the user a highly flexible and reliable automation, chemagen has developed a unique separation method together with the Forschungszentrum Karlsruhe . The isolation of nucleic acid molecules is achieved through their capturing by highly specific binding M-PVA Magnetic …. ...
TY - CHAP. T1 - 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. AU - Novopashina, D. S.. AU - Semikolenova, O. A.. AU - Venyaminova, A. G.. PY - 2020/1/1. Y1 - 2020/1/1. N2 - Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.. AB - Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and ...
In the last decade the use of field-effect-based devices has become a basic structural element in a new generation of biosensors that allow label-free DNA analysis. In particular, ion sensitive field effect transistors (FET) are the basis for the development of radical new approaches for the specific detection and characterization of DNA due to FETs greater signal-to-noise ratio, fast measurement capabilities, and possibility to be included in portable instrumentation. Reliable molecular characterization of DNA and/or RNA is vital for disease diagnostics and to follow up alterations in gene expression profiles. FET biosensors may become a relevant tool for molecular diagnostics and at point-of-care. The development of these devices and strategies should be carefully designed, as biomolecular recognition and detection events must occur within the Debye length. This limitation is sometimes considered to be fundamental for FET devices and considerable efforts have been made to develop better architectures
The advancement of gene-based therapeutics to the clinic is limited by the ability to deliver physiologically relevant doses of nucleic acids to target tissues safely and effectively. Over the last couple of decades, researchers have successfully e
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La terapia génica presenta potenciales aplicaciones terapéuticas para el tratamiento de muchas enfermedades como el cáncer, enfermedades monogénicas, enfermedad vascular, entre otras. Aunque la mayoría de protocolos de terapia génica empleen vectores virales, debido a su alta eficacia de transfección, crecientes preocupaciones debido a su activación de respuesta inmunológica motivan al desarrollo de sistemas de transportes no virales que sean seguros y eficaces. Poli(β-aminoestere)s (pBAEs) son prometedores vectores no virales debido a que su naturaleza de poliéster resulta en un atractivo perfil de biocompatibilidad debido a su alta biodegradabilidad y baja toxicidad. Este trabajo desarrolla una nueva familia de pBAEs, los cuales presentan oligopéptidos terminales, capaces de condensar tanto ADN como siRNA en partículas de tamaño nanométrico. En primer lugar, se realizan experimentos in vitro para evaluar la habilidad de estos nuevos pBAEs para transportar eficazmente tanto DNA ...
None, 11/7/2018, · Furthermore, PPE is often a legal requirement and it is the responsibility of the employer to ensure employees ,wear protective clothing, and observe safety and health regulations. It is also a responsibility, which employees must take seriously. The Employers Responsibility Towards Their …
Although the vast majority of such ligand binding activities or enzymatic activities known are performed by proteins a secondary subset of these fall under the category of Functional nucleic acids (FNAs). FNAs are RNA, ssDNA, or XNA(nucleic acid analogues) that perform an activity such as binding or catalyzing a reaction. FNAs are grouped into three main categories Aptamers, Ribozymes, and Deoxyribozymes that are further classified into either natural or artificial depending on their origin. The exception being Deoxyribozymes as they have yet to be discovered in a living organism. Still, It was only in the 1980s that the 1st ribozyme was discovered that we started to study FNAs and have allowed for the discovery of new methods, such as the SELEX or In vitro selection process that we are expanding their potential both as tools for exploring biology and real world problem solving. Note to self: mention RNA World ...
Functional nucleic acids (FNAs) are RNA, DNA, or XNA(nucleic acid analogues) that perform an activity such as binding or catalyzing a reaction. FNAs are grouped into three main categories Aptamers, Ribozymes, and Deoxyribozymes that are subdivided into either natural or artificial depending on their origin; the exception being Deoxyribozymes as they have yet to be discovered in a living organism. ...
Proper understanding of nucleic acid structure and function is critical as nucleic acids emerge as major targets and therapeutic mediators of physiological changes. Efficient, repeatable, and flexible computational construction methods for nucleic acids are largely unavailable, in part due to the magnitude of such a challenge. In this thesis, a novel suite of programs has been developed for construction, manipulation, and analysis of nucleic acids. This suite contains the following programs: NASDAC (Nucleic Acids: Structure, Dynamics, And Conformations), NASNIC (Nucleic Acid Structural Nomenclature Interpreter for Conformations), NASNOX (Nucleic Acid Structure modification: NitrOXide labels), and a higher-level program called NATCAR (Nucleic Acids: Topology, Conformation, Analysis, Refinement) through which all programs are launched. NASDAC is the primary engine of nucleic acid structure assembly, and NASNIC employs an original nucleic acid nomenclature named NAUTILIS (Nucleic Acids: Universal ...
The last decade has seen rapid development in single molecule manipulation of RNA and DNA. Measuring the response force for a particular manipulation has allowed the free energies of various nucleic acid structures and configurations to be determined. Optical tweezers represent a class of single mol …
Kolbing Frank (kolbing at fmi.ch) wrote: : I am searching for programs able to reveal antigenic sites in a given : protein sequence. I know of two programs that include this: # Name=predict.exe [ 2Nov93, 82kb] Path=9/EMBnet BioInformation Resource EMBL/Software/dos/predict Host=felix.embl-heidelberg.de # Name=sqaid381.exe [ 2Nov93, 208kb] Path=9/EMBnet BioInformation Resource EMBL/Software/dos/sqaid381 Host=felix.embl-heidelberg.de # Both are PD/Shareware and are using the antigenicity calculation proc. of Welling et.al 1985. Predict shows a nice graph on this, but lacks output other than plotter. Seqaid does a lot on nucleic acid analysis, as well as protein analysis. The output is ascii graphics, but also raw data. These can be easily imported in gnuplot or ACE/gr (or other) for high quality graphics output. Hope this helps, Sebastian -- email: [ Sebastian.Bunka at vu-wien.ac.at ] voice: FAX: +43-1-71155260 +43-1-7149110 Location: earth, europe, austria, vienna Inst. of Bacteriology Vet.Univ ...
How do you research viruses that don’t grow |em>in vitro|/em>? You go straight to ATCC synthetic nucleic acids. Now featuring Dengue, Norovirus, and West Nile Virus!
work presented in this thesis concerns studies on the physicochemical nature of interactions between nucleic acids and small ligands. The outcome of such studies can yield insights at a molecular level into the physiological mechanisms of action of biologically active nucleic-acid binding molecules. The thesis work includes investigations of a number of such low molecular weight compounds designed for nucleic acid sequence probing or therapeutic use. The interactions have been characterised by means of various optical spectroscopic techniques - including linear dichroism, circular dichroism and fluorescence - as well as nuclear magnetic resonance spectroscopy.. The fluorescent dye 4,6-diamidino-2-phenylindole (DAPI) is known to adopt different DNA binding modes in regions containing consecutive AT base-pairs as compared to those consisting of long sequences of GC base-pairs. In mixed sequence DNA, DAPI exhibits a pronounced preference to bind in the minor groove of AT rich regions. To verify ...
This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA…
Research Summary. Structural DNA Nanotechnology. Synthetic nucleic acid assemblies can now be programmed to self-assemble with high structural fidelity using Watson-Crick base-pairing. This synthetic structural approach offers unprecedented control over the 3D architecture and chemical composition of large-scale macromolecular assemblies that can also be interfaced with natural and synthetic molecules inside and outside of the cell. Here, we are developing computational strategies to enable high-throughput and high fidelity design and synthesis of arbitrary geometries, sizes, and sequences of DNA-based nanostructures for diverse applications in nanobiotechnology. In related work we are exploring use of these scaffolds for organizing toxins, viral coat proteins, chromophores, enzymes, lipids, and RNAs in complex 3D architectures for applications ranging from cellular drug targeting and delivery to biosensing and chemical synthesis. This work is funded by the ONR, NSF, and HFSP.. Programmed ...
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