The Global Isothermal Nucleic Acid Amplification Technology (INAAT) Market is expected to reach USD 4.3 billion by 2025, growing at a CAGR of 11.8%, according to a new report by Grand View Research, Inc. This can be attributed to significant rise in awareness regarding need for rapid and on-time testing for chronic and infectious diseases. Global increase in adoption of INAAT for detection of HIV, hepatitis A & B, and sexually transmitted diseases is considered to be a high impact rendering driver for growth.. Loop-Mediated Isothermal Amplification is one of the most preferred nucleic acid amplification methods because of its ability to provide point of care testing, thus leading to increased adoption. The recently-introduced Nicking Enzyme Amplification Reaction (NEAR) and Helicase-dependent amplification (HDA) technologies are expected to experience exponential growth during the forecast period owing to their patent protection.. In addition, owing to recent epidemics of infectious diseases, ...
The Global Isothermal Nucleic Acid Amplification Technology (INAAT) Market is expected to reach USD 4.3 billion by 2025, growing at a CAGR of 11.8%, according to a new report by Grand View Research, Inc. This can be attributed to significant rise in awareness regarding need for rapid and on-time testing for chronic and infectious diseases. Global increase in adoption of INAAT for detection of HIV, hepatitis A & B, and sexually transmitted diseases is considered to be a high impact rendering driver for growth.. Loop-Mediated Isothermal Amplification is one of the most preferred nucleic acid amplification methods because of its ability to provide point of care testing, thus leading to increased adoption. The recently-introduced Nicking Enzyme Amplification Reaction (NEAR) and Helicase-dependent amplification (HDA) technologies are expected to experience exponential growth during the forecast period owing to their patent protection.. In addition, owing to recent epidemics of infectious diseases, ...
The Global Isothermal Nucleic Acid Amplification Technology (INAAT) Market is expected to reach USD 4.3 billion by 2025, growing at a CAGR of 11.8%, according to a new report by Grand View Research, Inc. This can be attributed to significant rise in awareness regarding need for rapid and on-time testing for chronic and infectious diseases. Global increase in adoption of INAAT for detection of HIV, hepatitis A & B, and sexually transmitted diseases is considered to be a high impact rendering driver for growth.. Loop-Mediated Isothermal Amplification is one of the most preferred nucleic acid amplification methods because of its ability to provide point of care testing, thus leading to increased adoption. The recently-introduced Nicking Enzyme Amplification Reaction (NEAR) and Helicase-dependent amplification (HDA) technologies are expected to experience exponential growth during the forecast period owing to their patent protection.. In addition, owing to recent epidemics of infectious diseases, ...
The Global market for Isothermal Nucleic Acid Amplification Technology is poised to reach $2.1 billion by the end of 2020 growing at a CAGR of 10.5%. The P
Rapid and Sensitive Detection of Salmonella spp. by Using a Loop-Mediated Isothermal Amplification Assay in Duck Carcass Sample - loop-mediated isothermal amplification;Salmonella spp.;screening;duck;
A Loop-Mediated Isothermal Amplification Assay and Sample Preparation Procedure for Sensitive Detection of Xanthomonas fragariae in Strawberry. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Read Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay, Archives of Virology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
TY - JOUR. T1 - Rapid detection of rifampicin-resistant Mycobacterium tuberculosis, based on isothermal DNA amplification and DNA chromatography. AU - Takarada, Yutaka. AU - Kodera, Takuya. AU - Kobayashi, Kumi. AU - Nakajima, Chie. AU - Kawase, Mitsuo. AU - Suzuki, Yasuhiko. N1 - Funding Information: This study is based on previous results obtained from a project subsidized by the New Energy and Industrial Technology Development Organization (NEDO) . The research was supported in part by a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan(MEXT) , the Joint Research Program of the Research Center for Zoonosis Control, Hokkaido University to YS, and in part by Japan Agency for Medical Research and Development (AMED) under Grant Number JP20jk0210005 , JP20jm0110021 and JP20wm0125008 to YS. Funding Information: We thank Dr. Aki Tamaru for providing DNA extracted from clinically isolated M. tuberculosis. This study is based on previous results obtained from a ...
Methicillin-resistant Staphylococcus aureus (MRSA) has become one of the most prevalent pathogens responsible for nosocomial infections throughout the world. As clinical MRSA diagnosis is concerned, current diagnostic methodologies are restricted by significant drawbacks and novel methods are required for MRSA detection. This study aimed at developing a simple loop-mediated isothermal amplification (LAMP) assay targeting on orfX for the rapid detection of methicillin-resistance Staphylococcus aureus (MRSA). The protocol was designed by targeting orfX, a highly conserved open reading frame in S. aureus. One hundred and sixteen reference strains, including 52 Gram-positive and 64 Gram-negative isolates, were included for evaluation and optimization of the orfX-LAMP assay. This assay had been further performed on 667 Staphylococcus (566 MRSA, 25 MSSA, 53 MRCNS and 23 MSCNS) strains and were comparatively validated by PCR assay using primers F3 and B3, with rapid template DNA processing, simple equipments
The emergence of biosensor has triggered a revolution in many fields.It is an analytical device that uses biological or biochemical reactions to detect analytes and mainly consists of biological component and sensor.The
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This study was designed to optimize and apply the use of loop-mediated isothermal amplification (LAMP) as an alternative to conventional polymerase chain reaction (PCR) for the detection of herpesvirus of turkeys (HVT) (FC 126 strain) in vaccinated and non-vaccinated poultry in Nigeria
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject-specific sections.
Chlamydiae are implicated in a variety of clinically and economically important diseases in livestock and companion animals. These bacteria are associated with abortion, conjunctivitis, encephalomyelitis, enteritis, pneumonia, and polyarthritis in ruminants. Infection with these bacteria is the most common cause of abortion in sheep and goats and also causes zoonotic infection in humans which, in pregnant women, can result in spontaneous abortion.
Koi Herpesvirus (KHV) affects both juvenile and adult common carp and koi, and is especially lethal to fry. The high mortalities caused by the disease have had a negative impact on the international koi trade. Different diagnostic techniques have been used to detect KHV, including: isolation of the virus in cell culture, electron microscopy, several PCR tests, ELISA and in situ hybridisation. All of these methods are time consuming, laborious and require specialised equipment. A rapid field diagnosis of KHV in common and koi carp was developed using loop-mediated isothermal amplification (LAMP). The LAMP reaction rapidly amplified nucleic acid with high specificity and efficiency under isothermal conditions using a simple water bath. Two methods of extracting DNA from host tissue were compared: extraction by boiling and by using a commercial extraction kit. A set of six primers - two inner primers, two outer primers and two loop primers - was designed from a KHV amplicon. The reaction conditions were
Abstract. Monitoring post-control transmission of schistosomes by examining humans becomes less effective as infection rates among humans decrease. Molecular monitoring of prepatent schistosome infection in snails by the polymerase chain reaction (PCR) has been used for studying human-to-snail transmission, and snail prepatent infection rates were found to correspond to infection prevalence and average intensity in human populations contacting the sites studied. We have now developed loop-mediated isothermal amplification (LAMP) assays for identifying Schistosoma mansoni and S. haematobium to facilitate large-scale evaluation of post-intervention transmission potential. LAMP primers were designed based on the Sm1-7 and DraI repeated sequences of the corresponding schistosomes, and amplification by LAMP of these 121-basepair highly abundant sequences provided a detection sensitivity of 0.1 fg of genomic DNA. When these LAMP assays were applied for examining infected laboratory snails, it was possible to
Because of the serological cross-reactivity among the flaviviruses, molecular detection methods, such as reverse-transcription polymerase chain reaction (RT-PCR), play an important role in the recent Zika outbreak. However, due to the limited sensitivity, the detection window of RT-PCR for Zika viremia is only about one week after symptom onset. By combining loop-mediated isothermal amplification (LAMP) and AC susceptometry, we demonstrate a rapid and homogeneous detection system for the Zika virus oligonucleotide. Streptavidin-magnetic nanoparticles (streptavidin-MNPs) are premixed with LAMP reagents including the analyte and biotinylated primers, and their hydrodynamic volumes are dramatically increased after a successful LAMP reaction. Analyzed by a portable AC susceptometer, the changes of the hydrodynamic volume are probed as Brownian relaxation frequency shifts, which can be used to quantify the Zika virus oligonucleotide. The proposed detection system can recognize 1 aM synthetic Zika ...
A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤1.8 M, compared with a PCR assay that was functional at concentrations of ,100 mM. The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform.. ...
BackgroundToxoplasma gondii (T. gondii) causes an important parasitic infection known as toxoplasmosis, which is a globally distributed important zoonosis. One of the major serious characteristics of T. gondii is its ability to manipulate the behavior of intermediate hosts. We performed a cross-sectional study to determine toxoplasmosis in schizophrenic patients, as one of the major neuropsychiatric disorders, using loop-mediated isothermal amplification (LAMP) technic by targeting parasite B1 gene.MethodsBlood samples were taken from 118 schizophrenic patients hospitalized in tow hospitals including Baharan, Clinic of Psychiatric Ali-ibn-Abi-Talib Hospital (in Zahedan City), and Amir-al Momenin Psychiatric Hospital (in Zabol City), Sistan and Baluchestan Province, southeast Iran in 2016. They were analyzed using LAMP, and compared with the previous data of nested-PCR and serology.ResultsOut of the 118 schizophrenic individuals, 56 patients (47.4%) were found to be infected with T. gondii. The diagnosis
As a first step in developing a quick, accurate and simple method for the diagnosis of red ring disease, the loop-mediated isothermal amplification (LAMP)-based identification procedure was applied to the causative agent, Bursaphelenchus cocophilus. Two LAMP primer sets were designed using two loci of ribosomal RNA genes, i.e., D2-D3 expansion segments of the large subunit (D2-D3 LSU), and internal transcribed spacers (ITS). Within those two sets of primers, the D2-D3 LSU primer set successfully yielded amplicons from B. cocophilus nematode lysate prepared from 3-year-old DESS-fixed specimens. The specificity of the primers was examined using 18 species of confamilial Aphelenchoididae nematodes and primer sensitivity was tested using a diluted series of B. cocophilus lysate. The primer set did not amplify the DNA from other aphelenchoidids, and sensitivity was achieved by 1:100 diluted B. cocophilus DNA (roughly 1/1500 of total DNA from a single third-stage juvenile).
In this study, a loop-mediated isothermal amplification (LAMP) assay was established to detect Toxoplasma gondii DNA in mice infected with T. gondii PRU strain. This LAMP assay was based on the sequence of highly repetitive B1 gene. The detect...
Laboratory diagnosis has relied on the identification of malaria parasites and parasite antigens in peripheral blood using Giemsa-stained microscopy or rapid diagnostic tests (RDTs) which lack analytical and clinical sensitivity. The aim of this study was to evaluate the performance of loop-mediated isothermal amplification (LAMP) for the diagnosis of malaria among malaria suspected pregnant women in Northwest Ethiopia. The authors conclude that this study showed higher sensitivity of LAMP compared to microscopy and RDT for the detection of malaria in pregnancy. Increased sensitivity and ease of use with LAMP in point-of-care testing for malaria in pregnancy was noted. LAMP warrants further evaluation in intermittent screening and treatment programmes in pregnancy.. ...
Vet Parasitol. 2013 Feb 18;192(1-3):98-103. doi: 10.1016/j.vetpar.2012.10.010. Epub 2012 Oct 23. Research Support, Non-U.S. Govt
The need to perform gene expression profiling using next generation sequencing and quantitative real-time PCR (qPCR) on small sample sizes and single cells is rapidly expanding. However, to analyse few molecules, preamplification is required. Here, we studied global and target-specific preamplification using 96 optimised qPCR assays. To evaluate the preamplification strategies, we monitored the reactions in real-time using SYBR Green I detection chemistry followed by melting curve analysis. Next, we compared yield and reproducibility of global preamplification to that of target-specific preamplification by qPCR using the same amount of total RNA. Global preamplification generated 9.3-fold lower yield and 1.6-fold lower reproducibility than target-specific preamplification. However, the performance of global preamplification is sufficient for most downstream applications and offers several advantages over target-specific preamplification. To demonstrate the potential of global preamplification we
In aquaculture, vibriosis is known as a major bacterial disease in fish culture systems and can cause considerable loss in terms of production and processing (Toranzo et al. 2005). Many Vibrio species have been recognized as fish pathogens that can cause infection with various symptoms. For example, Vibrio scophthalmi infection results in hemorrhage on fish body surface and inner surface of the abdomen, severe enteritis, and ascites (Qiao et al. 2012); Vibrio ichthyoenteri infection cause opaque intestines and necrotizing fasciitis with high mortality rates (Ishimaru et al. 1996; Lee et al. 2012); Vibrio parahaemolyticus infection causes diseases not only in fish, shrimp, oysters, and mussels, etc. (Montilla et al. 1994; Quintoil et al. 2007), but also is important in public health and causes gastrointestinal disorders in humans who ingest contaminated fish and shellfish (Kubota et al. 2008; Iwamoto et al. 2010); and Vibrio vulnificus has been associated with vibriosis outbreaks in fish and ...
Battling infection is a major healthcare objective. Untreated infections can rapidly evolve toward the condition of sepsis in which the body begins to fail and resuscitation becomes critical and tenuous. Identification of infection followed by rapid antimicrobial treatment are primary goals of medical care, but precise identification of offending organisms by current methods is slow and broad spectrum empirical therapy is employed to cover most potential pathogens. Current methods for identification of bacterial pathogens in a clinical setting typically require days of time, or a four- to eight-hour growth phase followed by DNA extraction, purification and PCR-based amplification. We demonstrate rapid (70-120 minutes) genetic diagnostics methods utilizing loop-mediated isothermal amplification (LAMP) to test for 15 common infection pathogen targets, called the Infection Diagnosis Panel (In-Dx). The method utilizes filtration to rapidly concentrate bacteria in sample matrices with lower bacterial loads
In this study, we constructed a novel tool for the identification of six Plasmodium parasites, including two P. ovale subspecies, using MinION™ portable sequencer and the LAMP method. We demonstrated that the method can achieve a comprehensive diagnosis of malaria even in resource-limited endemic regions. In particular, the LAMP method employs a set of four specially designed primers that recognize six distinct sequences on the target DNA, and relies on an auto-cycling procedure under isothermal conditions. In addition, our method enabled rapid library preparation (within 30 min), with multiplex sequencing and streaming analysis of real-time sequencing data via the Rapid Barcoding Sequencing Kit. Therefore, our LAMP method combined with the MinION™ sequencer would be convenient to use in various clinical settings.. Recently, Loopamp™ MALARIA Pan/Pf Detection Kit (Eiken Chemical) has become commercially available for the diagnosis of malaria by the LAMP method. This LAMP kit has good ...
0002] Nucleic acid amplification technologies, such as a polymerase chain reaction (hereinafter, referred to as PCR) that requires a temperature change in reaction liquid amplification and detection processes and a loop mediated isothermal amplification method (hereinafter, referred to as LAMP method) that does not require a temperature change in reaction liquid amplification and detection processes, have been used for amplification and quantification of nucleic acids contained in samples originating in living organisms. For nucleic acid amplification, PCR requires periodically changing a sample temperature in typically about two to three temperature regions. For example, a typical PCR is performed as follows: the sample temperature is heated up to 94° C. to separate the double strands, followed by annealing at 60° C., and the sample temperature is kept at 60° C. to 72° C. for a few minutes. This PCR process is repeated by n times to amplify a target nuclear acid. On the other hand, in ...
Martzy R, Kolm C, Krska R, Mach RL, Farnleitner AH, Reischer GH (2019) Challenges and perspectives in the application of isothermal DNA amplification method for food and water analysis.Analytical and Bioanalytical Chemistry 144:9, 1695-1702. Martzy R, Bica-Schröder K, Pálvölgyi AM, Kolm C, Jakwerth S, Kirschner AKT, Sommer R, Krska R, Mach RL, Farnleitner AH, Reischer GH (2019) Simple lysis of bacterial cells for DNA-based diagnostics using hydrophilic ionic liquids.Scientific Reports 9:13994. Martzy R, Kolm C, Brunner K, Mach RL, Krska R, S?inkovec H, Sommer R, Farnleitner AH, Reischer GH (2017) A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Enterococcus spp. in water.Water Research 122:62-69. Georg Kerber (2019) 3D Modellierung des Grundwasserkörpers für den Bereich des Brunnenfeldes Donauinsel Nord.Technische Universität Wien, Diplomarbeit, 2019 ...
A47 Recently, GD-100 (SYSMEX, Kobe, Japan) has been released. It rapidly can detect and quantify mRNA by one-step nucleic acid amplification (OSNA). Loop-mediated isothermal amplification (LAMP) is performed and turbidity derived from magnesium pyrophosphate formation with LAMP can be monitored in OSNA. LAMP originally was reported in 2000 as a novel nucleic acid amplification method that uses only one type of enzyme and yields 109 of its target in less than an hour. In this study, we compared the sensitivity of OSNA using GD-100 versus conventional real-time RT-PCR, using five colon cancer cell lines. We then applied OSNA to detect micro-metastases in 1) lymph nodes (LNs) from patients with advanced colorectal adenocarcinomas; and 2) LNs submitted for frozen diagnosis from patients with several malignancies. Our goal was to develop a more efficient system to detect LN metastases than histological examination (HE) performed intra-operatively.Methods: CK19 mRNA copy numbers of five colon ...
Multiple displacement amplification (MDA) is a widely used technique for amplification of DNA from samples containing limited amounts of DNA (e.g., uncultivable microbes or clinical samples) before whole genome sequencing. Despite its advantages of high yield and fidelity, it suffers from high amplification bias and non-specific amplification when amplifying sub-nanogram of template DNA. Here, we present a microfluidic digital droplet MDA (ddMDA) technique where partitioning of the template DNA into thousands of sub-nanoliter droplets, each containing a small number of DNA fragments, greatly reduces the competition among DNA fragments for primers and polymerase thereby greatly reducing amplification bias. Consequently, the ddMDA approach enabled a more uniform coverage of amplification over the entire length of the genome, with significantly lower bias and non-specific amplification than conventional MDA. For a sample containing 0.1 pg/μL of E. coli DNA (equivalent of ~3/1000 of an E. coli genome per
Researchers showed how an isothermal amplification technique could detect S. enterica serovars from culture by targeting specific gene markers.
Discoveries of new human viruses and new technologies for their detection have made, and will continue to make, major contributions to the safety of blood transfusion. This article discusses the practical issues involved in the implementation of additional serological screening tests for viruses such as human T-lymphotropic virus, and reviews current information on the prevalence and pathogenicity of more recently discovered viruses, such as hepatitis G virus (HGV) or GB virus-C (GBV-C) and human herpes virus 8, a potential aetiological agent of Kaposis sarcoma. Progress in the technology behind nucleic acid amplification techniques, such as the polymerase chain reaction (PCR), makes direct detection of viruses such as human immunodeficiency virus and hepatitis C virus possible. The use of such methods for screening will allow the earlier detection of acutely-infected individuals and the elimination of transmission from window period donations before seroconversion for antibody. Establishing a
Viruses such as hepatitis A virus (HAV), noroviruses (NoV), sapoviruses, enteroviruses, astroviruses, adenoviruses, rotaviruses, and hepatitis E virus have all been implicated in food- and/or water-borne outbreaks of illness. This chapter deals with NoV and HAV detection in bivalve mollusks, soft fruits, and water. For bivalve mollusks, fecal indicators are measured either in the shellfish themselves or in their growing waters. When virus detection procedures are mentioned, the recurrent issue of detecting infectious or physical particles comes into discussion. Whenever possible, infectious assays coupled with identification methods are preferred for direct assessment of human health risk. Nucleic acid amplification techniques are currently the most widely used methods for detection of viruses in food and water and also enable investigators to gather information on the virus genotypes occurring in the environment and in food products, thus providing the most relevant epidemiological information,
Currently available diagnostic approaches for tuberculosis are limited by low sensitivity and time factors. The present gold standard for diagnosis is isolation of positive culture from clinical specimens, which generally takes up to 8 weeks for results. Unfortunately, even with rapid culture techniques, the results take at least 1 week to return, which increases the difficulty of identifying infected patients and ensuring that they follow-up for treatment. Nucleic acid amplification techniques are limited by their sensitivity; they are presently widely recommended only for AFB-smear (+) patients. The EliSpot assay, which detects IFN-g producing T cells specific for mTB, cannot reliably distinguish between active and latent TB given that the test is performed on peripheral blood samples. Therefore, rapid, highly sensitive tests are desperately needed in order to identify patients infected with TB and limit the transmission of this potentially fatal infection. We have developed an innovative ...
3. 233 Hepatitis C virus (HCV), validation of nucleic acid amplification techniques for the detection streett HCV RNA in plasma pools Guidelines.
Molecular diagnostics refers to a technique used to detect and identify the presence of genetic material or proteins associated with a specific health condition or disease. Viral molecular diagnostic helps in diagnosis of infectious diseases caused by virus. Viral molecular diagnostics has varied applications in hospitals, academics institutions, laboratories and others. On the basis of application, viral molecular diagnostics can be segmented into infectious diseases, genetics, blood screening, microbiology and others. On the basis of infectious diseases, viral molecular diagnostics market can be segmented into hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV) human papillomavirus (HPV) and others. On the basis of technology, viral molecular diagnostics market can be segmented into polymerase chain reaction (PCR), isothermal nucleic acid amplification technology (INAAT), microarrays, in situ hybridization and others.. North America, followed by Europe, has the ...
Sampling technique and detection rates of oropharyngeal and anorectal gonorrhoea using nucleic acid amplification tests in men who have sex with men ...
Global Zika Virus Testing Market By Product Type (Nucleic Acid Amplification Test/ Molecular Tests, Zika Virus Antibody Test/ Serological Test) And By End-Users/Application (Hospitals, Diagnostic Centers) Global Market Share, Forecast Data, In-Depth Analysis, And Detailed Overview, and Forecast, 2013 - 2026
We tested our hypothesis that double strand breaks can improve the loading efficiency of the helicase near a target sequence by evaluating the impact of restriction endonuclease digestion during HDA amplification. As shown in Figure 1B, specific restriction enzyme can be selected to cleave a specific sequence near the target sequence, generate 5ss (single-stranded) ends, 3ss ends or blunt ends to help recruiting and loading the helicase. Therefore, one simple protein for mediation of helicase homing can replace the functions of multiple accessory proteins in vivo. Indeed, previous studies had already used restriction enzymes to produce substrates for helicase unwinding assay in vitro [12, 13]. So far more than 3000 restriction endonucleases with over two hundred different specificities have been isolated from bacteria [14]. This broad collection makes finding an enzyme which can cut the DNA duplex close to the target very straightforward. The time-saver qualified enzymes from New England ...
Abbott ID NOW A rapid, instrument-based system for the qualitative detection of infectious diseases using isothermal nucleic acid amplification technology. Shop Abbott™ ID
A unit dose test device for a nucleic acid amplification reaction has the form of a elongate disposable test strip. The test strip includes a dual-chamber reaction vessel pre-loaded with nucleic acid amplification reaction reagents, and a plurality of wells for processing a reaction occurring in the reaction vessel.
Genetically modified (GM) rice KMD1, TT51-1, and KF6 are three of the most well known transgenic Bt rice lines in China. A rapid and sensitive molecular assay for risk assessment of GM rice is needed. Polymerase chain reaction (PCR), currently the most common method for detecting genetically modified organisms, requires temperature cycling and relatively complex procedures. Here we developed a visual and rapid loop-mediated isothermal amplification (LAMP) method to amplify three GM rice event-specific junction sequences. Target DNA was amplified and visualized by two indicators (SYBR green or hydroxy naphthol blue [HNB]) within 60 min at an isothermal temperature of 63 °C. Different kinds of plants were selected to ensure the specificity of detection and the results of the non-target samples were negative, indicating that the primer sets for the three GM rice varieties had good levels of specificity. The sensitivity of LAMP, with detection limits at low concentration levels (0.01%-0.005% GM), was 10-
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of equine coronavirus (ECoV). This assay was conducted at 60°C for 40min. Specificity of the RT-LAMP assay was confirmed using several equine intestinal and respiratory pathogens in addition to ECoV. The novel assay failed to cross-react with the other pathogens tested, suggesting i ...
Biological RNA generally comprises secondary structure motifs which cause a problem for target RNA detection by isothermal amplification methods. The complexity of the secondary structures makes RNA targets inaccessible for probe hybridization, resulting in decreased sensitivity and selectivity. This is particularly important because the hybridization step of the isothermal amplification method re ...
Pathologic lymph node staging is becoming a deficient method in the demanding molecular era. Nevertheless, the use of more sensitive molecular analysis for nodal staging is hampered by its high costs and extensive time requirements. Our aim is to take a step forward in colon cancer (CC) lymph node (LN) pathology diagnosis by proposing a feasible and efficient molecular method in routine practice using reverse transcription loop-mediated isothermal amplification (RT-LAMP). Molecular detection of tumor cytokeratin 19 (CK19) mRNA with RT-LAMP was performed in 3206 LNs from 188 CC patients using two methods: individual analysis of 1449 LNs from 102 patients (individual cohort), and pooled LN analysis of 1757 LNs from 86 patients (pooling cohort). A median of 13 LNs (IQR 10-18) per patient were harvested in the individual cohort, and 18 LNs (IQR 13-25) per patient in the pooling cohort (p ≤ 0.001). The median of molecular assays performed in the pooling cohort was 2 per patient (IQR 1-3), saving a median
Open peer review is a system where authors know who the reviewers are, and the reviewers know who the authors are. If the manuscript is accepted, the named reviewer reports are published alongside the article. Pre-publication versions of the article and author comments to reviewers are available by contacting [email protected] All previous versions of the manuscript and all author responses to the reviewers are also available.. You can find further information about the peer review system here.. ...
Mediators of Inflammation is a peer-reviewed, Open Access journal that publishes original research and review articles on all types of inflammatory mediators, including cytokines, histamine, bradykinin, prostaglandins, leukotrienes, PAF, biological response modifiers and the family of cell adhesion-promoting molecules.
The vision of point-of-care pathogen detection has become more achievable since the development of isothermal nucleic acid amplification techniques. These techniques rapidly produce large quantities of nucleic acids to enable high sensitivity of detection, whilst only requiring a single temperature of operation. Recombinase polymerase amplification (RPA) is a relatively new isothermal amplification technique amenable for use in low-resource settings. Detection can be achieved using lateral flow devices, components are supplied freeze-dried in single use ampoules, and the assay operates at 37 °C. However, little is known about the tolerance of RPA for the detection of heterogeneous sequence populations. Indeed, the extended length requirements for RPA primers and probe can make it more difficult to find near-exact regions of conservation within diverse sequence populations. Here we describe an RPA assay for detection of Japanese encephalitis virus (JEV), which comprises 4 lineages and 7 clusters, with
FIG 2 Detection sensitivity of RT-LAMP, RT-PCR, and real-time RT-PCR for New Bunyavirus. RT-LAMP products from virus serial dilutions were visualized by adding FDR dye under visible light (A), UV light (B), and by agarose gel electrophoresis (C). RT-PCR products were evaluated by agarose gel electrophoresis (D) and real-time RT-PCR amplification (E). Lane 1, negative control; lanes 2 to 8, NBV samples from 101 to 107 TCID50/ml serial dilutions; lane 9, positive control; lane M, DL2000 molecular size marker. ...
The present invention is directed to a rapid and sensitive method for detecting Ureaplasma urealyticum using U. urealyticum-specific probes and oligonucleotides. In particular a target sequence can be amplified by in vitro nucleic acid amplification techniques or directly detected by nucleic acid hy ...
The control and detection of foodborne pathogens is a major problem in public health. Therefore, the development of rapid and economical diagnostic method along the food chain has become a priority. Among the available methodologies, those based on the detection of nucleic acids provide certain advantages such as high sensitivity speed, selectivity, and the ability to simultaneously detect multiple microorganisms. However, the amount of pathogen DNA present in a food is minimal, so these methods must incorporate an amplification stage that achieves the proper amount for their detection. The polymerase chain reaction (PCR) is the common technique, although this method has the requirement of thermocycling. In this master project two enzymatic reactions that enable the amplification of DNA under isothermal conditions have been studied. The work focuses on the comparison of conventional PCR with two isothermal amplification methods ‐recombinase polymerase (RPA) and multiple displacement ...
TY - JOUR. T1 - Rapid and low-cost amplicon visualization for nucleic acid amplification tests using magnetic microbeads. AU - Nakano, Michihiko. AU - Inaba, Masafumi. AU - Suehiro, Junya. N1 - Funding Information: This work was partly supported by the following foundations: Yoshida Foundation for the Promotion of Learning and Education, Mukai Science and Technology Foundation, and the Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering. It was also partly supported by the Incubation Center for Advanced Medical Science of Kyushu University, and the Center for Clinical and Translational Research of Kyushu University Hospital. Publisher Copyright: © The Royal Society of Chemistry 2021.. PY - 2021/5/7. Y1 - 2021/5/7. N2 - This study presents a rapid and low-cost amplicon detection method in which amplicons are attached to magnetic microbeads, suspended in deionized water, and subjected to a magnetic field on a hydrophilic surface resulting in the circular ...
ITS2 DNA Sequence Analysis for Eight Species of Delphacid Planthoppers and a Loop-mediated Isothermal Amplification Method for the Brown Planthopper-specific Detection. Bo Yoon Seo, Chang Gyu Park, Young-Ho Koh, Jin Kyo Jung, Jumrae Cho, Chanyeong Kang. Korean Journal of Applied Entomology :: Vol.56 No.4 pp.377-385. DOI:https://doi.org/10.5656/KSAE.2017.10.0.033 ...
The detection of lymph node metastasis by RT-PCR (37-40) and by QRT-PCR (12, 19-25) has been studied previously. CK19 mRNA has been described as having the highest sensitivity at nearly 90%. However, there are drawbacks using CK19 mRNA due to the concomitant amplification of pseudogenes in genomic DNA that lead to false positive results. For this reason, a combination of two or three markers has been used.. We evaluated 45 potential mRNAs and finally selected CK19 mRNA as the best marker for the OSNA assay. To use CK19 mRNA as a marker, we designed RT-LAMP primers that do not amplify the known CK19 pseudogenes (see Materials and Methods). In addition, the lymph node solubilization step in the OSNA assay was carried out at pH 3.5. At this pH, almost all genomic DNA precipitates out. Even when the sample still contained genomic DNA, DNA amplification is unlikely to occur in the OSNA assay because the RT-LAMP step is carried out at 65°C, a temperature at which genomic DNA typically does not ...
TY - JOUR. T1 - Characterization of the 12q Amplicons in Lipomatous Soft Tissue Tumors by Multiplex Ligation-dependent Probe Amplification-based Copy Number Analysis. AU - Creytens, David. AU - Van Gorp, Joust. AU - Speel, Ernst-Jan. AU - Ferdinande, Liesbeth. PY - 2015/4. Y1 - 2015/4. KW - Lipoma. KW - well-differentiated liposarcoma. KW - dedifferentiated liposarcoma. KW - pleomorphic liposarcoma. KW - multiplex ligation-dependent probe amplification. KW - 12q amplicon. M3 - Article. VL - 35. SP - 1835. EP - 1842. JO - Anticancer Research. JF - Anticancer Research. SN - 0250-7005. IS - 4. ER - ...
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To combine the advantages of molecular testing (sensitivity) and immunoassays (low cost), we developed an assay for toxigenic C. difficile that couples isothermal DNA amplification to array-based hybridization. In lieu of monitoring nucleic acid amplification in real time, this approach permits inexpensive detection, requiring only a digital image instead of fluorophore-based detection with accompanying sophisticated optics and algorithms. Multiplexing is accomplished at two levels: at the amplification step and via hybridization to capture probes immobilized on the array. These methods were sufficient for detection of fewer than 10 C. difficile CFU in the context of a fecal sample. The ability of HDA to amplify crude fecal samples is also seen with other crude samples, for example, blood culture (22). Straightforward filtration and automated dilution produce a simple test in which a swab sample is filtered and transferred into the cartridge to initiate testing.. To discriminate toxigenic from ...
TEONG, TEOH BOON (2015) Colorimetric detection of dengue by single tube reverse- transcription-loop-mediated isothermal amplification. PLoS One, 10 (9). e0138694. TEONG, TEOH BOON (2015) Detection of Langat virus by TaqMan real-time one-step qRT- PCR method. Scientific Reports, 5. p. 14007. TEONG, TEOH BOON (2015) Early detection of dengue virus by use of reverse transcription-recombinase polymerase amplification. Journal of Clinical Microbiology, 53 (3). pp. 830-7. TEONG, TEOH BOON (2015) High producing tumor necrosis factor alpha gene alleles in protection against severe manifestations of dengue. International Journal of Medical Sciences, 12 (2). pp. 177-86. TEONG, TEOH BOON (2015) The IL-10 and IL-12B gene polymorphisms in the multiethnic Malaysian population. Genetics and Molecular Research, 14 (2). pp. 3257-63. TEONG, TEOH BOON (2014) Baicalin, a metabolite of baicalein with antiviral activity against dengue virus. Scientific Reports, 4. p. 5452. TEONG, TEOH BOON (2013) Comparison of real ...
Evaluation of Recombinase Polymerase Amplification (RPA) Isothermal Amplification Diagnostic Assay for Phytophthora ramorum.
It was recently demonstrated that recombinaseᅠpolymeraseᅠamplification (RPA), an isothermal amplification platform for pathogen detection, may be used to quantify DNA sampleᅠconcentrationᅠusing aᅠstandard curveᅠIn this manuscript, a detailed protocol for developing and implementing a real-time quantitative recombinase polymerase amplification assay (qRPA assay) is provided. Using HIV-1 DNA quantification as an example, the assembly of real-time RPA reactions, the design of an internal positive control (IPC) sequence, and co-amplification of the IPC and target of interest are all described. Instructions and data processing scripts for the construction of a standard curve using data from multiple experiments are provided, which may be used to predict the concentration of unknown samples or assess the performance of the assay. Finally, an alternative method for collecting real-time fluorescence data with a microscope and a stage heater as a step towards developing a point-of-care qRPA ...
The clinical outcomes and cost implications of a diagnostic shift from an EIA- to PCR-based assay for Clostridium difficile infection (CDI) have not been completely described in the literature. The impact of the PCR-based assay on the incidence and duration of CDI therapy was compared to the EIA assay for patients with a negative CDI diagnostic result. Secondary clinical and economic outcomes were also evaluated. Independent predictors of receipt of antibiotic therapy were assessed via logistic regression. 141 EIA and 140 PCR patients were included. Significantly more patients were started or continued on anti-CDI antibiotic therapy after a known negative assay result in the EIA group (26 patients vs. 8 patients, P = 0.002). Duration of antibiotic therapy after a known negative result was significantly shorter in the PCR group (1 vs. 4 days, P = 0.029) and a 23% reduction in the number of tests obtained per patient was observed (1.41 ± 0.86 vs. 1.82 ± 1.35, P = 0.007). The over fourfold difference in
In another embodiment, the products of the amplification process may be bound to an immobilized support, hybridized to a nucleic acid probe containing a complementary sequence, and separated from the unhybridized nucleic acid probe which remains in solution. The products, DNA or RNA, may be bound directly to a solid support by any stable interaction, such as hydrophobic, electrostatic, or covalent interaction. In addition, the products may contain certain chemical groups, for example, biotin, which may be incorporated into the products during the amplification process to allow binding to an immobilized protein, for example, avidin or streptavidin. In addition, the products may be hybridized to a nucleic acid which contains a complementary sequence and which can be immobilized. The nucleic acid probe would contain a complementary sequence which forms a sufficiently stable interaction with a product of the amplification process to allow binding under the conditions of hybridization and sustained ...
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Detection methods included chromogenic media (powdered media and prepared plates), Immunoassays, and various forms of molecular methods including simple polymerase chain reaction (PCR), multiplex PCR, real-time PCR, nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP), and oligonucleotide DNA microarray. The reason that so many vendors focused on pathogen screening is because while if looking at the number of performed tests, pathogen testing accounts for less than 20% of the assays; while from the laboratory budget it accounts for almost 50% of the budget. The most important features of the new assays shown in order of importance accuracy (bases of sensitivity and specificity, and also inclusivity and exclusivity, limit-of-detection (LOD)), speed (total time to actionable result), range of food matrices (the ability of the method to be utilized in a broad range of food matrices), and cost of the assay and instrument. Another important criterion is the ...
The Xpert MTB/RIF is a cartridge based nucleic acid amplification test, automated diagnostic test that can identify Mycobacterium tuberculosis (MTB) DNA and resistance to rifampicin (RIF) by nucleic acid amplification test (NAAT). It was co-developed by the laboratory of Professor David Alland at the University of Medicine and Dentistry of New Jersey (UMDNJ). Cepheid Inc. and Foundation for Innovative New Diagnostics, with additional financial support from the US National Institutes of Health (NIH). In December 2010, the World Health Organization (WHO) endorsed the Xpert MTB/RIF for use in TB endemic countries. This followed 18 months of assessment of its field effectiveness in TB, MDR-TB and TB/HIV co-infection. This test, and others that are likely to follow, could have the potential to improve the diagnosis of TB in those that are likely to be missed by traditional tests. Tuberculosis is one of the deadliest public health threats today. Traditionally, tuberculosis is mostly being diagnosed by ...
Evolution of HCV infection markers over time.. Most infected people are asymptomatic with onset of disease being insidious. Symptoms range from anorexia, fatigue, fever, myalgia, nausea and vomiting progressing to jaundice. More than 50% of those infected will develop chronic HCV infection. Of those chronically infected about half will develop cirrhosis or cancer of the liver.. Hepatitis C virus (HCV) is an RNA virus of the Flaviviridae family, previously known as NANB hepatitis, which is spread predominantly by parenteral routes. An estimated 170 million people are infected worldwide, and HCV infection is now the leading cause for liver transplantation in the United States because of its propensity to cause chronic liver disease, cirrhosis, and hepatocellular carcinoma.. HCV is recognized as the cause of most cases of post-transfusion hepatitis and is a significant cause of morbidity and mortality worldwide. HCV infection has been reported in virtually every country where it has been carefully ...
Immunoliposomes and use thereof in highly specific and sensitive nucleic acid amplification assays relying on amplification of specific nucleic acid sequences released from encapsulation within a lipo
The invention provides a rapid, sensitive and specific nucleic acid detection system which utilizes isothermal nucleic acid amplification in combination with a lateral flow chromatographic device, or DNA dipstick, for DNA-hybridization detection. The system of the invention requires no complex instrumentation or electronic hardware, and provides a low cost nucleic acid detection system suitable for highly sensitive pathogen detection. Hybridization to single-stranded DNA amplification products using the system of the invention provides a sensitive and specific means by which assays can be multiplexed for the detection of multiple target sequences.
Quidel has received 510(k) clearance from the FDA to market Quidels new Solana Strep Complete Assay for the rapid and qualitative detection and differentiation of Streptococcus pyogenes (Group A beta-hemolytic Streptococcus) and Streptococcus dysgalactiae (pyogenic Group C and G beta-hemolytic Streptococcus) nucleic acids isolated from throat swab specimens obtained from symptomatic patients. The Solana molecular platform leverages the Helicase-Dependent Amplification technology in Quidels AmpliVue molecular product line to generate a fast and accurate test result. The device can process up to 12 patient samples in . . .
Flow cytometry facilitates sorting of single chromosomes and chromosome arms which can be used for targeted genome analysis. However, the recovery of microgram amounts of DNA needed for some assays requires sorting of millions of chromosomes which is laborious and time consuming. Yet, many genomic applications such as development of genetic maps or physical mapping do not require large DNA fragments. In such cases time-consuming de novo sorting can be minimized by utilizing whole-genome amplification. Here we report a protocol optimized in barley including amplification of DNA from only ten thousand chromosomes, which can be isolated in less than one hour. Flow-sorted chromosomes were treated with proteinase K and amplified using Phi29 multiple displacement amplification (MDA). Overnight amplification in a 20-microlitre reaction produced 3.7 - 5.7 micrograms DNA with a majority of products between 5 and 30 kb. To determine the purity of sorted fractions and potential amplification bias we used
BACKGROUND: Sustainable DNA resources and reliable high-throughput genotyping methods are required for large-scale, long-term genetic association studies. In the genetic dissection of common disease it is now recognised that thousands of samples and hundreds of thousands of markers, mostly single nucleotide polymorphisms (SNPs), will have to be analysed. In order to achieve these aims, both an ability to boost quantities of archived DNA and to genotype at low costs are highly desirable. We have investigated phi29 polymerase Multiple Displacement Amplification (MDA)-generated DNA product (MDA product), in combination with highly multiplexed BeadArray genotyping technology. As part of a large-scale BeadArray genotyping experiment we made a direct comparison of genotyping data generated from MDA product with that from genomic DNA (gDNA) templates. RESULTS: Eighty-six MDA product and the corresponding 86 gDNA samples were genotyped at 345 SNPs and a concordance rate of 98.8% was achieved. The BeadArray
Background: Sustainable DNA resources and reliable high-throughput genotyping methods are required for large-scale, long-term genetic association studies. In the genetic dissection of common disease it is now recognised that thousands of samples and hundreds of thousands of markers, mostly single nucleotide polymorphisms (SNPs), will have to be analysed. In order to achieve these aims, both an ability to boost quantities of archived DNA and to genotype at low costs are highly desirable. We have investigated Φ29 polymerase Multiple Displacement Amplification (MDA)-generated DNA product (MDA product), in combination with highly multiplexed BeadArray™ genotyping technology. As part of a large-scale BeadArray genotyping experiment we made a direct comparison of genotyping data generated from MDA product with that from genomic DNA (gDNA) templates. Results: Eighty-six MDA product and the corresponding 86 gDNA samples were genotyped at 345 SNPs and a concordance rate of 98.8% was achieved. The BeadArray
Here we describe a method for the detection of Clostridium difficile from stool using a novel low-complexity and rapid extraction process called Heat Elution (HE). The HE method is two-step and takes just 10 minutes, no specialist instruments are required and there is minimal hands-on time. A test method using HE was developed in conjunction with Loop-mediated Isothermal Amplification (LAMP) combined with the real-time bioluminescent reporter system known as BART targeting the toxin B gene (tcdB). The HE-LAMP-BART method was evaluated in a pilot study on clinical fecal samples (tcdB+, n = 111; tcdB−, n = 107). The HE-LAMP-BART method showed 95.5% sensitivity and 100% specificity against a gold standard reference method using cytotoxigenic culture and also a silica-based robotic extraction followed by tcdB PCR to control for storage. From sample to result, the HE-LAMP-BART method typically took 50 minutes, whereas the PCR method took |2.5 hours. In a further study (tcdB+, n = 47; tcdB−, n = 28) HE
Transparency Market Research Reports included a detailed market survey and analysis trends on Nucleic Acid Testing Market. This report also includes more info about basic overview of the industry including definitions, applications and global market industry structure.. Nucleic acid tests are performed in order to detect the presence of viral DNAs or RNAs in blood samples of patients. Three major processes involved in NAT are sample pooling and nucleic acid (NA) extraction, target NA amplification and target amplicon NA detection.Major NAT technologies include polymerase chain reaction (PCR), strand displacement amplification (SDA), ligase chain reaction (LCR), transcription-mediated amplification (TMA) and nucleic acid sequence based amplification (NASBA). Based on its major applications, NAT market can be classified into five major segments namely infectious diseases, genetic diseases, cancer, forensic testing and paternity testing.. Browse the full report with request TOC at ...
Scientists from the University of Oxfords Nuffield Department of Medicine have today published their evaluation of LamPORE, a novel diagnostic platform for detecting SARS-CoV-2 RNA. It combines loop-mediated isothermal amplification with nanopore sequencing. This technology has the potential to analyse thousands of samples per day on a single instrument.
Zhang, Jian-min et al. Detection of Haemophilus parasuis isolates from South China by loop-mediated isothermal amplification and isolate characterisation. Onderstepoort j. vet. res., 2012, vol.79, no.1, p.01-06. ISSN 0030- ...
This invention relates to the use of tumor-derived or associated extracellular ribonucleic acid (RNA) found circulating in the plasma or serum fraction of blood for the detection, monitoring, or evaluation of cancer or premalignant conditions. Extracellular RNA may circulate as non-bound RNA, protein-bound RNA, lipid-RNA complexes, lipoprotein (proteolipid)-RNA complexes, protein-RNA complexes including within or in association with ribonucleoprotein complexes, nucleosomes, or within apoptotic bodies. Any intracellular RNA found in plasma or serum can additionally be detected by this invention. Specifically, this invention enables the extraction of circulating RNA from plasma or serum and utilizes nucleic acid amplification assays for the identification, detection, inference, monitoring, or evaluation of any neoplasm, benign, premalignant, or malignant, in humans or other animals, which might be associated with that RNA. Further, this invention allows the qualitative or quantitative detection of tumor
Looking for amplification system? Find out information about amplification system. Complements are words or groups of words that are necessary to complete the meaning of another part of the sentence. Complements act like modifiers to add... Explanation of amplification system
BACKGROUND: Xpert MTB/RIF Ultra (Xpert Ultra) and Xpert MTB/RIF are World Health Organization (WHO)-recommended rapid nucleic acid amplification tests (NAATs) widely used for simultaneous detection of Mycobacterium tuberculosis complex and rifampicin resistance in sputum. To extend our previous review on extrapulmonary tuberculosis (Kohli 2018), we performed this update to inform updated WHO policy (WHO Consolidated Guidelines (Module 3) 2020). OBJECTIVES: To estimate diagnostic accuracy of Xpert Ultra and Xpert MTB/RIF for extrapulmonary tuberculosis and rifampicin resistance in adults with presumptive extrapulmonary tuberculosis. SEARCH METHODS: Cochrane Infectious Diseases Group Specialized Register, MEDLINE, Embase, Science Citation Index, Web of Science, Latin American Caribbean Health Sciences Literature, Scopus, ClinicalTrials.gov, the WHO International Clinical Trials Registry Platform, the International Standard Randomized Controlled Trial Number Registry, and ProQuest, 2 August 2019 ...
When people go for either a Chlamydia test or a Gonorrhea test, they always go for a combined Chlamydia and Gonorrhea test.. The main reason for that is that both the tests are quite similar. Additionally, Chlamydia symptoms are very similar to Gonorrhea symptoms, so its only wise to test for both infections together to ascertain the exact type of infection.. This test is called the Nucleic Acid Amplification Test or NAAT. Both Chlamydia and Gonorrhea are caused by bacteria. When somebody is infected with these bacteria, theres supposed to be the presence of the bacteria in the urine sample.. Chlamydia and Gonorrhea Test Window Period is generally 3 to 7 days. It means 3 to 7 days after the infection period the detection of the individual bacteria can be done positively. In most of the cases people come for a test much after that, but if youre not among them then its better that you wait for at least 7 to 10 days.. The NAAT test carries out an amplification of the DNA of the bacteria and ...
The APTIMA HPV Assay is an in vitro nucleic acid amplification test for the qualitative detection of E6/E7 viral messenger RNA (mRNA) from 14 high-risk types of human papillomavirus (HPV) in cervical specimens. The high-risk HPV types detected by the assay include: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68. The APTIMA HPV Assay does not discriminate between the 14 high-risk types. Cervical specimens collected in ThinPrep Pap Test vials containing PreservCyt® Solution with commercially available collection devices (broom-type device or cytobrush/spatula combination) may be tested with the APTIMA HPV Assay either pre- or post-Pap processing. The assay is used with the TIGRIS System and the PANTHER System.. The objective is to establish that APTIMA HPV Assay performance on the PANTHER System is comparable to performance on the TIGRIS System. ...
OBJECTIVE: Voice-aligned compression (VAC) is a method used in Oticons hearing aids to provide more comfortable hearing without sacrificing speech discrimination. The complex, non-linear compression curve for the VAC strategy is designed based on the frequency profile of certain spoken Western languages. We hypothesized that hearing aids could be further customized for Japanese-speaking users by modifying the compression curve using the frequency profile of spoken Japanese. METHODS: A double-blind randomized controlled crossover study was performed to determine whether or not Oticons modified amplification strategy (VAC-J) provides subjectively preferable hearing aids for Japanese-speaking hearing aid users compared to the same companys original amplification strategy (VAC ...
Submit only 1 of the following specimens:. Swab specimen must be collected using an Aptima Collection Unisex Swab (T583), or Aptima Collection Multitest Swab (T584, formerly called Aptima Vaginal Swab Specimen Collection Kit). These swabs are contained in the Aptima Collection Kit.. Supplies: Swab, Aptima Male/Female Collection (T583). Specimen Type: Endocervix. Container/Tube: Aptima Collection Unisex Swab (T583). Specimen Volume: Swab. Collection Instructions:. 1. Use cleaning swab (white shaft) to remove excess mucus from endocervix and discard.. 2. Insert second swab (blue shaft) 1 to 1.5 cm into endocervical canal, and rotate swab gently for 30 seconds. Avoid touching vaginal wall when removing swab.. 3. Place second swab into transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.. 4. Cap tube securely, and label tube with patients entire name, and date and time of collection.. 5. Transport and store swab container at 2 to 30° C (refrigerate ...
PrimeIAmp™ COVID-19 Lyophilized Fluorescence Nucleic Acid Detection Kit utilizes world-class isothermal amplification technology to detect COVID-19 nucleic acid by quantitative PCR
Diagnose the acute retroviral syndrome. The most appropriate next diagnostic test is an HIV nucleic acid amplification test. This patients medical history and timing of symptoms are typical of acute HIV infection. Although his symptoms could also represent infectious mononucleosis or syphilis, preliminary results for those conditions are negative. Most persons in whom HIV infection develops experience an acute symptomatic illness within 2 to 4 weeks of infection. Symptoms typically last for a few weeks and range from a simple febrile illness to a full-blown mononucleosis-like syndrome. Because patients lack an immune response during this period, virus levels tend to be very high, resulting in high levels of infectivity. Symptoms of acute HIV infection resolve with or without treatment, and most acute infections are undiagnosed. Patients presenting with symptomatic acute HIV infection (the acute retroviral syndrome) are usually in the window period, which may extend for 3 to 6 weeks, during ...
Dear Ani,. Various types of sexually transmitted infections (STIs) require different tests. A urine test can be used to check for chlamydia and gonorrhea, two of the most common bacterial STIs.. A urine test or urinalysis involves collecting a urine sample which is then sent to a laboratory to be analyzed. At the lab, technicians look for evidence of chlamydia or gonorrhea in the urine using various tests. The lab chooses the correct test based on technical capabilities of the lab, cost, and other factors. One option is a nucleic acid amplification test (NAAT), which is used to make extra copies of any nucleic acids or genetic material present in the urine sample. By amplifying the genetic material, lab technicians can more easily identify any parts that belong to chlamydia or gonorrhea bacteria. Another test called nucleic acid hybridization (NAH) works kind of like Velcro by using a DNA probe to seek out and attach to the rRNA material of the chlamydia or gonorrhea bacteria. A third test ...
ARRIVING PASSENGERS 1. 3 If a symptomatic person has a low likelihood of SARS-CoV-2 infection, clinical discretion should determine if this negative antigen test result requires confirmatory testing 7 Nucleic acid amplification test; confirm within 48 hours using a NAAT, such as RT-PCR, that has been evaluated against FDAs reference panel for analytical sensitivity operating procedure COVID-19 virus testing in NHS laboratories This guidance is correct at the time of publishing. Berlin, Jan 17th, 2020 Diagnostic detection of 2019-nCoV by real-time RT-PCR -Protocol and preliminary evaluation as of Jan 17, 2020- Victor Corman, Tobias Bleicker, Sebastian Brünink, Christian Drosten Charité Virology, Berlin Coronavirus (COVID-19) support Check for travel restrictions. This COVID-19 test detects certain proteins in the virus. Besides surgery on a COVID‐19 patient posing a risk to the treatment team, it can also be detrimental to the post‐operative course of COVID‐19 for the patient 3 . PCR ...
The LAMP method is comparable to PCR (polymerase chain reaction) in terms of sensitivity and specificity. In contrast to PCR however, the LAMP method is less costly and less affected by the presence of non-target DNA and inhibitory contaminants. LAMP does not require sophisticated equipment and reagents. Also, it can be used with simple heating devise such as water bath or heat block. Because of its high specificity and visiual indicators of the presence of a pathogen (Notomi et al, 2000) the LAMP method is considered a potential tool for field diagnosis of swine diseases. Continue reading LAMP for Rapid Detection of Common Pathogens causing disease in Swine. ...
The LAMP method is comparable to PCR (polymerase chain reaction) in terms of sensitivity and specificity. In contrast to PCR however, the LAMP method is less costly and less affected by the presence of non-target DNA and inhibitory contaminants. LAMP does not require sophisticated equipment and reagents. Also, it can be used with simple heating devise such as water bath or heat block. Because of its high specificity and visiual indicators of the presence of a pathogen (Notomi et al, 2000) the LAMP method is considered a potential tool for field diagnosis of swine diseases. Continue reading LAMP for Rapid Detection of Common Pathogens causing disease in Swine. ...
Results In 2011, a total of 550 cases of gonorrhoea were reported giving a rate of 26.7 per 100 000 population. In 2012 - 602 cases and an incidence of 29.5. In total, the incidence of gonorrhoea increased by 73.5% from 2010 to 2012. For detection of N. gonorrhoeae, currently three laboratories are using nucleic acid amplification tests (NAATs), 10 - RNA probe hybridization tests, four laboratories culture, and 17 - Gram staining of specimen smears only. Monitoring of AMR in N. gonorrhoeae was initiated in 2010, however, during 2010 and 2012 in total only 35 isolates were tested. All 35 isolates were fully susceptible to cefixime, ceftriaxone, azithromycin, and spectinomycin, and the minimum inhibitory concentrations (MICs) of gentamicin were low. Resistance to ciprofloxacin was displayed by 43% of isolates, to penicillin by 31%, and to tetracycline by 23%.. ...
Recombinase polymerase amplification (RPA) is an isothermal amplification technique. Because of its short detection cycle and high specificity, it has been applied in various fields. However, the design of probe on the efficiency of RPA is not well understood and the effect of sequence mismatches of oligonucleotides on the performance of RPA is rarely discussed. In this study, we found that different primers with the same probe have a slight effect on the efficiency of fluorescent RPA, and different probes with the same amplified region have a great influence on the efficiency of fluorescent RPA. We summarized the design rules of probes suitable for fluorescent RPA by analyzing the experimental data. The rule is that the best distance between fluorescent groups in the probe is 1-2 bases, and the G content should be reduced as far as possible. In addition, we verified this rule by designing a series of probes. Furthermore, we found the base mismatches of the probe had a significant effect on RPA, ...