Procedures for the isolation of HeLa S3 nuclear matrices were re-examined with special emphasis on the use of various nucleases and detergents as well as on the ionic strength of the final salt extraction. The protein composition of the resulting nuclear matrix preparations was analysed by one- and two-dimensional gel electrophoresis and found to be extremely reproducible. By means of co-electrophoresis several typical cytoskeletal proteins (actin, vimentin and cytokeratins) and heterogeneous nuclear RNA (hnRNA)-associated core proteins (hnRNP) were shown to be present in such nuclear matrix preparations. The nature of some other protein components was elucidated using two-dimensional immunoblotting and immunofluorescence. For this purpose mouse monoclonal antibodies to cytoskeletal components (vimentin, cytokeratins), small nuclear RNP (70 X 10(3) Mr protein of U1-RNP), hnRNP (C1/C2) and the pore-complex lamina (lamins A, B and C) were used next to human autoimmune sera obtained from patients ...
Numerous reports have suggested that disturbances in nuclear condensation may result in male infertility. This notion has been supported by the observation of infertile individuals with a decrease or absence of the male sperm-specific chromatin packaging protamine proteins. To date, no correlation between the absence of protamine proteins and a mutation within the coding regions of the protamine genes has been documented. To address this issue, PCR-based mutation scanning analysis has been performed across the human male haploid expressed PRM1--|PRM2--|TNP2 domain in several oligozoospermic infertile individuals. This analysis identified a candidate mutation in a region of contact with the sperm nuclear matrix from 2 of 5 affected individuals. This is the first report of a mutation scan covering the entire PRM1--|PRM2--|TNP2 locus in affected individuals.
TY - JOUR. T1 - Identifying Nuclear Matrix-Attached DNA Across the Genome. AU - Dobson, Jason R.. AU - Hong, Deli. AU - Barutcu, A. Rasim. AU - Wu, Hai. AU - Imbalzano, Anthony N.. AU - Lian, Jane B.. AU - Stein, Janet L.. AU - van Wijnen, Andre J. AU - Nickerson, Jeffrey A.. AU - Stein, Gary S.. PY - 2016. Y1 - 2016. N2 - Experimental approaches to define the relationship between gene expression and nuclear matrix attachment regions (MARs) have given contrasting and method-specific results. We have developed a next generation sequencing strategy to identify MARs across the human genome (MAR-Seq). The method is based on crosslinking chromatin to its nuclear matrix attachment sites to minimize changes during biochemical processing. We used this method to compare nuclear matrix organization in MCF-10A mammary epithelial-like cells and MDA-MB-231 breast cancer cells and evaluated the results in the context of global gene expression (array analysis) and positional enrichment of gene-regulatory ...
PHELAN, THOMAS JOSEPH, Genetic and Molecular Analysis of Plant Nuclear Matrix Proteins. (Under the direction of Steven L. Spiker.)The eukaryotic nucleus is composed of DNA, RNA and protein, encapsulated by a nuclear envelope. DNA is compacted up to ten thousand times in order to be packaged into the nucleus. The nucleus must maintain order in the presence of a very high density and variety of protein and RNA. The nuclear matrix is a proteinaceous network thought to provide structure and organization to the nucleus. We believe that relatively stable interactions of nuclear molecules with the nuclear matrix are key to organization of the nucleus. Numerous "Matrix Attachment Region" DNA elements (MARs), have been isolated from plants, animals, and fungi. Evidence suggests that these MARs attach to the nuclear matrix, delimiting loops of chromosomal DNA. In studies of transgenic plants and animals, MARs have been shown to give important advantages to organisms transformed with genes flanked by these ...
The overall objective of this study has been to address some of the longstanding questions concerning functional organization of the interphase nucleus. This was achieved by using recently developed high-resolution fluorescence in situ hybridization techniques for a precise localization of specific DNA and RNA sequences in conjunction with immunocytochemistry and biochemical fractionation. This study is based on the philosophy that new insights may be gained by an approach that attempts to interrelate genomic organization, spatial arrangement of RNA metabolism, and nuclear substructure within the mammalian cell nucleus. The nuclear distribution of an exogenous, viral RNA (Epstein-Barr Virus, EBV) within nuclear matrix preparations was studied by developing an approach which couples in situ hybridization with biochemical fractionation procedures. EBV RNA molecules accumulate in highly localized foci or elongated tracks within the nucleus of lymphoma cells. These RNA tracks were retained with spatial and
TY - JOUR. T1 - The specific binding of estradiol to the nuclear matrix.. AU - Barrack, E. R.. AU - Hawkins, E. F.. AU - Coffey, D. S.. PY - 1979. Y1 - 1979. N2 - Recent studies on the eukaryotic nucleus have described the presence of a structural component termed the nuclear matrix, that appears to be involved in nucleic acid synthesis. This study will present evidence that the nuclear matrix of both the rat uterus and chick liver contains specific, high affinity, saturable estradiol binding sites.. AB - Recent studies on the eukaryotic nucleus have described the presence of a structural component termed the nuclear matrix, that appears to be involved in nucleic acid synthesis. This study will present evidence that the nuclear matrix of both the rat uterus and chick liver contains specific, high affinity, saturable estradiol binding sites.. UR - http://www.scopus.com/inward/record.url?scp=0018336689&partnerID=8YFLogxK. UR - ...
To determine whether or not initiation sites for DNA replication in mammalian cells are defined by association with nuclear structure, attachments between the nucleoskeleton and the hamster DHFR gene initiation zone were examined. Nucleoskeletons were prepared by encapsulating cells in agarose and then extracting them with a nonionic detergent in a physiological buffer. The fraction of DNA that remained following endonuclease digestion was resistant to salt, sensitive to Sarkosyl, and essentially unchanged by glutaraldehyde crosslinking. Although newly replicated DNA was preferentially attached to the nucleoskeleton, no specific sequence was preferentially attached within a 65 kb locus containing the DHFR gene, two origins of bi-directional replication and at least one nuclear matrix attachment region. Instead, the entire region went from preferentially unattached to preferentially attached as cells progressed from G1 to late S-phase. Thus, initiation sites in mammalian chromosomes are not ...
The nuclear matrix protein, NMP-2, was originally identified as an osteoblast-specific DNA-binding complex localized exclusively to the nuclear matrix. NMP-2 was shown to recognize two binding sites, site A (nt-605 to -599) and site B (nt -441 to -435), in the rat bone-specific osteocalcin gene promoter. This study shows that the NMP-2 binding sites A and B as well as a third NMP-2 binding site (nt -135 to -130) constitute a consensus sequence, ATGCTGGT, and represent an AML-1 recognition motif. AML-1 is a member of the AML transcription factor family which is associated with acute myelogenous leukemia and binds to the sequence TGCTGGT via its DNA-binding runt domain. Electrophoretic mobility shift assays reveal that a component of NMP-2 is a member of the AML/PEBP2/runt domain transcription factor family based on cross-competition with AML-1 consensus oligonucleotide. Limited immunoreactivity of NMP-2 with a polyclonal N-terminal AML-1 antibody and inability of the AML-1 partner protein CBF-beta to
Our laboratory recently completed the first comprehensive proteomic analysis of a Gram-negative biofilm matrix. This work demonstrated that a subset of secreted...
The DNase I accessibility and chromatin organization of genes within the nucleus do correlate to their transcriptional activity. Here, we show that both serum starvation and overexpression of Tip5, a key regulator of ribosomal RNA gene (rDNA) repression, dictate DNase I accessibility, facilitate the association of rDNA with the nuclear matrix and thus regulate large-scale rDNA chromatin organization. Tip5 contains four AT-hooks and a TAM (Tip5/ARBP/MBD) domain, which were proposed to bind matrix-attachment regions (MARs) of the genome. Remarkably, the TAM domain of Tip5 functions as nucleolar localization and nuclear matrix targeting module, whereas AT-hooks do not mediate association with the nuclear matrix, but they are required for nucleolar targeting. These findings suggest a dual role for Tip5s AT-hooks and TAM domain, targeting the nucleolus and anchoring to the nuclear matrix, and suggest a function for Tip5 in the regulation of higher-order rDNA chromatin structure. ...
Three early proteins expressed by adenovirus type 5, E1b 55K, E4 Orf3 and E4 Orf6, are involved in regulating late viral gene expression. It has previously been shown that 55K associates with Orf6. Here we show that 55K also associates with Orf3 and that this interaction is necessary for 55K to localize to the nuclear matrix fraction of the cell. From our data, we infer that the Orf3 and Orf6 interactions with 55K may be mutually exclusive. The Orf3 protein is also known to associate with and cause the reorganization of cell nucleus structures known as ND10 or PODs. Consistent with the observed increase in the biochemical interaction between 55K and Orf3 in the absence of Orf6, the 55K association with Orf3 in ND10 was also found to increase in the absence of Orf6. The most studied cellular component of ND10 is PML, a complex protein present in a range of isoforms, some of which are modified by conjugation to the small ubiquitin-like protein PIC-1. The pattern of PML isoforms was altered in adenovirus
CDCP1 a transmembrane protein containing three extracellular CUB domains involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation depending upon its phosphorylation status. Interacts with N- and P-cadherin, syndecans 1 and 4, the serine protease ST14, SRC and PKCG. Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Its expression level is correlated with the metastatic ability of carcinoma cells. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. Also expressed in cells with phenotypes reminiscent of mesenchymal stem ...
The absorption and fluorescence spectra of silver trimers isolated in an Ar matrix show the existence of three distinct lattice sites whereby the population of each site depends on the conditions of matrix preparation. Irradiation of certain absorption bands of the trimer isolated in one of these sites results in an irreversible reorganization of the surrounding matrix and population of another site. The experiments were performed with matrices prepared either by the matrix isolation/aggregation method or by soft landing of size-selected Ag/sub 3/ species.. ...
Some of the IGFBPs have been proposed to potentiate IGF actions by forming extracellular matrix associations, facilitating transport to cell-surface receptors,
The nucleotide sequence of the UL31 open reading frame is predicted to encode a basic protein with a hydrophilic amino terminus and a nuclear localization signal. To identify its gene product, we constructed a viral genome in which the thymidine kinase gene was inserted between the UL31 and UL32 open reading frames. The thymidine kinase gene was then deleted, and in the process, the 5 terminus of the UL31 open reading frame was replaced with a 64-bp sequence in frame with the complete, authentic sequence of the UL31 open reading frame. The inserted sequence encoded a hydrophilic epitope derived from glycoprotein B of human cytomegalovirus and for which a monoclonal antibody is available. We report that in infected cells, the tagged protein localized in and was dispersed throughout the nucleus. Nuclear fractionation studies revealed that the UL31 protein partitions with the nuclear matrix. The protein is phosphorylated in infected cells maintained in medium containing 32Pi. ...
Myocardial injury in mammals leads to heart failure through pathological cardiac remodelling that includes hypertrophy, fibrosis and ventricular dilatation. Central to this is inability of the mammalian cardiomyocyte to self-renew due to entering a quiescent state after birth. Modulation of the cardiomyocyte cell-cycle after injury is therefore a target mechanism to limit damage and potentiate repair and regeneration. Here we show that cardiomyocyte specific over-expression of the nuclear-matrix associated DNA replication protein, CIZ1, extends their window of proliferation during cardiac development, delaying onset of terminal differentiation without compromising function. CIZ1 expressing hearts are enlarged, but the cardiomyocytes are smaller with an overall increase in number, correlating with increased DNA replication after birth and retention of an increased proportion of mono-nucleated cardiomyocytes into adulthood. Furthermore, these CIZ1 induced changes in the heart reduce the impact of ...
TPZ is an anticancer agent designed to exploit the unique hypoxic microenvironment of solid tumors (4) . At low levels of oxygen, the TPZ radical, produced by intracellular reduction of the parent drug, leads to extensive DNA damage including base damage, DNA SSBs, and DNA DSBs, the latter of which can account for the cytotoxicity of the drug (29 , 43) . Recent studies have shown the nuclear metabolism of TPZ is not only responsible for all of the damage caused by TPZ (12) but is also compartmentalized with different reductases being responsible for the production of DNA SSBs and DSBs (14) . We hypothesized, based on the finding that TPZ is metabolized by reductases associated with the nuclear matrix (14) , that this selective localization of TPZ reduction could lead to a disruption of the components of the nuclear matrix or of the activities that take place there. This protein-rich scaffold maintains organization of the chromatin loops and aids in critical nuclear functions, such as DNA ...
Principal Investigator:SUGANO Nobuhiko, Project Period (FY):1995 - 1997, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Functional biochemistry
The human nuclear matrix proteins (hNMP) have reassembling and potential filament forming capabilities. The hNMP200 has a 56kDa nuclear protein that…
Principal Investigator:TSUTSUI Ken, Project Period (FY):1989 - 1990, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:General medical chemistry
The residing matrix is outlined as the continual molecular cloth in the organism, consisting of fascia, the opposite connective tissues, extracellular matrices, integrins, cytoskeletons, nuclear matrices and DNA. The extracellular, mobile and nuclear biopolymers or ground substances constitute a human body-huge reservoir of charge that will retain electrical homeostasis and "inflammatory preparedness" throughout the organism. Current investigation has emphasized the importance of charge transfer in relation on the scavenging or neutralization of no cost radicals delivered to web pages of harm for the duration of and following the oxidative burst. Proof originates from studies in the part of electrons in mitigating the implications of inflammation when residing methods are linked to the earth (earthing ...
Because chromosomal DNA is tethered by attachments to the nuclear matrix every 50kb or so (Ward et al. Development over a path of 15 cm.
Substitution of thymine with 5-bromouracil in DNA is known to change interaction between DNA and proteins, thereby inducing various biological phenomena. We hypothesize that A/T-rich scaffold/nuclear matrix attachment region (S/MAR) sequences are involved in the effects of 5-bromodeoxyuridine. We examined an interaction between DNA containing an intronic S/MAR sequence of the immunoglobulin heavy chain gene and nuclear halos prepared from HeLa cells. Upon substitution with 5-bromouracil, the S/MAR DNA bound more tightly to the nuclear halos. The multi-functional nuclear matrix protein YY1 was also found to bind more strongly to 5-bromouracil-substituted DNA containing its recognition motif. These results are consistent with the above hypothesis.. ...
Recently we have shown that in vitro binding of the proximal part of the human tyrosine hydroxylase gene to the nuclear matrix is correlated with its transcriptional activity. The strongest binding potential was predicted by computing for the first intron sequence (Lenartowski & Goc, 2002, Neurosci Lett.; 330 : 151-154). In this study a 16 kb fragment of the bovine genomic DNA containing the tyrosine hydroxylase gene was investigated for its affinity to the nuclear matrix. Only a 950 bp fragment encoding the distal part of the first intron, second exon and a few nucleotides of the second intron bound to the nuclear matrix. The binding was independent of the tissue-specific tyrosine hydroxylase gene activation. The fragment was subcloned and sequenced. Computer search pointed to one potential intronic matrix attachment region with two AP1-like sites embedded in the sequence. We conclude that even if the position of the matrix binding region is conserved among the tyrosine hydroxylase genes in ...
The genotoxicity of reactive oxygen species (ROS) is well established. The underlying mechanism involves oxidation of DNA by ROS. However, we have recently shown that hydrogen peroxide (H2O2), the major mediator of oxidative stress, can also cause genomic damage indirectly. Thus, H2O2 at pathologically relevant concentrations rapidly induces higher order chromatin degradation (HOCD), i.e. enzymatic excision of chromatin loops and their oligomers at matrix-attachment regions. The activation of endonuclease that catalyzes HOCD is a signalling event triggered specifically by H2O2. The activation is not mediated by an influx of calcium ions, but resting concentrations of intracellular calcium ions are required for the maintenance of the endonuclease in an active form. Although H2O2-induced HOCD can efficiently dismantle the genome leading to cell death, under sublethal oxidative stress conditions H2O2-induced HOCD may be the major source of somatic mutations.. ...
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Fig. 3. CIZ1 promotes CM mono-nucleation and DNA replication. (A) Proportion of mono-, bi-, tri- and tetra-nucleated CMs in WT (white) and Tg (green) hearts at 12 weeks (n=,500 CM/mouse, four mice/group), with representative examples of GFP-positive (green) Tg nuclei. (B) Examples of partially divided GFP-positive nuclei (arrows). (C) Hearts of 2-week-old Tg mice had threefold more replicating CMs than WT, detected following incorporation of EdU for 48 h (n=10-12 sections/mouse, ,1000 nuclei, three mice/group). A slight decrease was observed in replicating NMs. (D) Examples of replicating Tg NMs (short arrow) and CMs (long arrow). Cell identity was based on nuclear size/position relative to WGA-stained membrane (Frentzou et al., 2015). (E) CM:NM ratio during development from neonate to young adulthood, in WT (white) and Tg (green) mice. Scale bar=20 μm. Data represented as mean±s.e.m. *P≤0.05, ***P≤0.001 by two-tailed t-test. ...
matrin 3 an acidic protein that is a component of the nuclear matrix. May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. Note: This description may include information from UniProtKB ...
2006年04月, DNA Structure, Chromatin and Gene Expression, Chapter 14, "Involvement of nuclear matrix and chromatin loop formation in the function of insulators.", Transworld Research Network, 2006年, 04, 単行本(学術書), 共編著, Akasaka K. , ...
How is SWI/SNF Related, Matrix-Associated, Actin-Dependent, Regulator of Chromatin abbreviated? SMARC stands for SWI/SNF Related, Matrix-Associated, Actin-Dependent, Regulator of Chromatin. SMARC is defined as SWI/SNF Related, Matrix-Associated, Actin-Dependent, Regulator of Chromatin somewhat frequently.
Using liposome flotation assays and a novel liposome bead binding assay, we have characterized the binding of HIV-1 MA to membranes containing different phospholipids, and our results (Fig. 2 to 5) support the notion (9, 17, 36, 41) that MA binds preferentially to PI(4,5)P2 versus PC, PS, or PI. We did not observe the level of PI(4,5)P2 selectivity shown with soluble headgroup analogues (41), but this may be due to the presence of membranes and/or the possibilities of multivalent binding in our studies. We also did not achieve the PI(4,5)P2 selectivity found in some studies with in vitro-translated HIV-1 PrGag proteins (9). This could be a consequence of higher protein-to-lipid ratios or the absence of the missing Gag domains in our system. However, it is worth noting that RNase treatment of in vitro translation extracts reduces PrGag selectivity for PI(4,5)P2-containing liposomes (A. Ono, University of Michigan, personal communication), and this effect of nucleic acids is discussed in more ...
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A felső légúti fertőzés a megfázás orvosi elnevezése. A megfázás az orr és a torok egyszerű fertőzését jelenti. Normál légzés esetén a levegő beáramli...
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A high-density matrix was prepared by coating an alumina particle with agarose using an emulsion technique. Iminodiacetic acid was immobilized onto this matrix. Charging this matrix with copper created a useful chromatography matrix for purification of indigenous sesame sprout lipase. Butanediol diglycidyl ether (BDGE) was used as spacer arm. Factors such as, adsorption pH, BDGE concentration in matrix preparation, concentration of NaCl and imidazole were investigated. Based on both the binding capacity of matrix and the eluate adsorbed lipase, the optimum BDGE concentration and the adsorption pH were found about 40 % and 7, respectively. However, an increase in NaCl concentration in adsorption buffer from 0.5 to 1.5 M resulted in 2 times decrease in the ratio between adsorbed lipase and adsorbed total proteins. More interestingly, immobilization of Cu on this matrix was highly effective in the purification lipase, since lipase could be easily eluted from matrix using low concentration of ...
The nuclear matrix is considered to play a significant role in the DNA replication of eukaryotic cells, although immediate evidence for such a job is inadequate still. GAL4-RAD and PEBP2B1. Moreover, AML1/ETO inhibited Py DNA replication stimulated by GAL4-RAD and Rabbit Polyclonal to KCNK15 PEBP2B1. The inhibition was particular for replication mediated by GAL4-RAD and PEBP2B1, and proportional to the amount of lack of these activators in the nuclear matrix, recommending a requirement of nuclear matrix concentrating on in the arousal of Py DNA replication by RAD. These email address details are the first ever to recommend a molecular hyperlink between your initiation of DNA replication as well as the nuclear matrix area. Accumulating proof suggests an participation of transcription elements in the legislation of DNA replication in eukaryotic cells. The polyomavirus (Py) DNA replication program is fantastic for elucidating the assignments of transcription elements in DNA replication, as Py DNA ...
Bioluminescent-labelling allows sensitive non-invasive sequential imaging of tumor development and early metastasis. Current methods for the genetic modification of cells typically use integrating genotoxic viruses that can potentially disrupt the molecular behavior of cancer cell lines due to their random nature of integration. VAL401 is the reformulation of a clinical drug to enable use in the treatment of cancer. Preclinical data indicate potential use of the reformulated drug in lung cancer, where many subsets of patients have currently a high unmet medical need. We have utilized a non-viral DNA vector that comprises an S/MAR (Scaffold/Matrix Attachment Region) element to stably modify cells to be further used in xenograft studies to allow long term expression without affecting cell behavior or silencing over cell divisions. Human BxPC3 pancreatic cancer cells were stably transfected with a pSMARt-UBC-Luc and cultured for 4 weeks under selection. Colonies that formed after this period were ...
In addition to Matrin3 and Raver1, we found a number of other interesting factors that bound to PTB RRM2 and were sensitive to the Y247Q mutation (Joshi et al, 2011), suggesting that their interactions might also be mediated by PRI motifs (Fig 1). These included splicing regulators, proteins involved in the co‐transcriptional‐dependent regulation of splicing and 3′ end processing factors. We did not identify a number of previously identified PTB interactors, including hnRNP‐L, PSF, hnRNPA1, hnRNPA2B1, hnRNPC and MRG15 (Patton et al, 1993; Hahm et al, 1998; Luco et al, 2010; King et al, 2014), although we did identify the helicase DDX3X (King et al, 2014). The lack of overlap could be because we focused on proteins that interact primarily via RRM2. Interesting novel PTB interactors with possible relevance for PTBs splicing regulatory activities include KIAA1967/DBC1/CCAR2 and its paralogue CCAR1, both of which bound strongly to PTB RRM2 in a manner that was sensitive to the PTB Y247Q ...
Gene expression is regulated not only by the swarm of transcription factors that interact with the promoter region of the gene but also by the nuclear localization of the gene. Matrix attachment regions (MARs) of DNA bind to the proteinaceous network of the nucleus and segregate chromosomal subdomains; the MARs may define loops of chromatin presenting multiple genes for coordinated activation or repression by soluble factors. In plants, the loops seem to be on the order of 10 kilobases in length. Surveying variability in expression from low-copy-number transgenes in maize, Brouwer et al. find that different MARs have diverse effects on gene expression, in some cases reducing transgene silencing, and an individual MAR could have multiple effects according to the developmental status of the cells being tested. The MARs offer a glimpse into a complex process, which can alter both chromatin structure and gene expression. - PJH. Plant Cell14, 2251 (2002).. ...
Although it is widely believed that eukaryotic DNA is looped by attachment to a nucleoskeleton, there is controversy about its composition and which sequences are attached to it. As most nuclear derivatives are isolated using unphysiological conditions, the criticism that attachments seen in vitro are generated artifactually has been difficult to rebut. Therefore we have re-investigated attachments of chromatin to the skeleton using physiological conditions. HeLa cells are encapsulated in agarose microbeads and lysed using Triton in a physiological buffer. Then, most chromatin can be electroeluted after treatment with a restriction enzyme to leave some at the base of the loops still attached. Analysis of the size and amounts of these residual fragments indicates that the loops are 80-90kbp long. The residual fragments are stably attached, with about 1kbp of each fragment protected from nuclease attack. This is very much longer than a typical protein-binding site of 10-20bp.
マウス・モノクローナル抗体 ab54370 交差種: Hu 適用: WB,Dot…Nuclear Matrix Protein p84抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
マウス・モノクローナル抗体 ab487 交差種: Ms,Hu 適用: WB,IP,IHC-P,IHC-Fr,ICC,Flow Cyt,ICC/IF…Nuclear Matrix Protein…
Calcium-binding and coiled-coil domain-containing protein 2 is a protein that in humans is encoded by the CALCOCO2 gene. The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. GRCh38: Ensembl release 89: ENSG00000136436 - Ensembl, May 2017 "Human PubMed Reference:". Korioth F, Gieffers C, Maul GG, Frey J (Jul 1995). "Molecular characterization of NDP52, a novel protein of the nuclear domain 10, which is redistributed upon virus infection and interferon treatment". J Cell Biol. 130 (1): 1-13. doi:10.1083/jcb.130.1.1. PMC 2120522 . PMID 7540613. "Entrez Gene: CALCOCO2 calcium binding and coiled-coil domain 2". Maruyama K, Sugano S ...
MORC3 Full-Length MS Protein Standard (NP_056173), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. This gene encodes a protein that localizes to the nuclear matrix. The protein also has RNA binding activity, and has a predicted coiled-coil domain.
Cikkszám: SH-SAW-FP Ideális élelmiszeripari előkészítő felületek, munkapultok, rozsdamentes acél konyhaedények és felszerelések tisztítására, fertőtlenítésére, sterilizálására. Tökéletesen használható élelmiszeripari, közétkeztetési és egyéb magán-, és nyilvános vendéglátóipari ágazatokban, konyhákon, éttermekben, élelmiszerüzemekben, hús,-hentesáru üzletekben.
I have read of placing fertilizer pellets in the planting hole when transplanting on installations. Anyone doing this and what do you use? Thanks,...
In molecular biology, the BEN domain is a protein domain which is found in diverse proteins including: SMAR1 (Scaffold/Matrix attachment region-binding protein 1; also known as BANP), a tumour-suppressor MAR-binding protein that down-regulates Cyclin D1 expression by recruiting HDAC1-mSin3A co-repressor complex at Cyclin D1 promoter locus; SMAR1 is the target of prostaglandin A2 (PGA2) induced growth arrest. NACC1, a novel member of the POZ/BTB (Pox virus and Zinc finger/Bric-a-bracTramtrack Broad complex), but which varies from other proteins of this class in that it lacks the characteristic DNA-binding motif. Mod(mdg4) isoform C, the modifier of the mdg4 locus in Drosophila melanogaster (Fruit fly), where mdg4 encodes chromatin proteins which are involved in position effect variegation, establishment of chromatin boundaries, nerve path finding, meiotic chromosome pairing and apoptosis. Trans-splicing of Mod(mdg4) produces at least 26 transcripts. BEND2, a protein of unknown function, that is ...
Link to Pubmed [PMID] - 9235947. J. Biol. Chem. 1997 Aug;272(31):19457-63. Transcriptional induction of genes is an essential part of the cellular response to interferons. We have established a cDNA library from human lymphoblastoid Daudi cells treated for 16 h with human alpha/beta-interferon (IFN) and made use of differential screening to search for as yet unidentified IFN-regulated genes. In the course of this study, we have isolated a human cDNA that codes for a 20-kDa protein sharing striking homology with the product of the Xenopus laevis XPMC2 gene. This new gene is induced by both type I and II IFNs in various cell lines and will be referred to as ISG20 for interferon-stimulated gene product of 20 kDa. Confocal immunofluorescence analysis of the subcellular localization of ISG20 protein reveals that it is closely associated with PML and SP100 gene products within the large nuclear matrix-associated multiprotein complexes termed the PML nuclear ...