The neurofibromatosis 2 protein merlin is a classical tumor suppressor protein. Germline mutations predispose to the development of schwannomas, meningiomas and ependymomas. Merlin has been implicated in cellular migration and adhesion. This function is reflected in its subcellular localization at the plasma membrane and known interacting partners. Merlin has been regarded as an exception in not exerting a functional role within the nucleus as other tumor suppressors do. Here, we show that detection of wild-type protein in the nucleus is a rare event. However, splicing out of exon 2 leads to unrestricted entry into the nucleus. Skipping of adjacent exon 3 has no comparable effect ruling out an unspecific effect due to misfolding of the 4.1/JEF domain. Exon 2 functions as a cytoplasmic retention factor as it is able to confer sole cytoplasmic localization to a GFP fusion protein. Nuclear entry of merlin is thus regulated by alternative splicing within the 4.1/JEF domain and analogous to band 4.1 ...

The neurofibromatosis 2 (NF2) tumor suppressor protein merlin is commonly mutated in human benign brain tumors. The gene altered in NF2 was located on human chromosome 22q12 in 1993 and the encoded protein named merlin and schwannomin. Merlin has homology to ERM family proteins, ezrin, radixin, and moesin, within the protein 4.1 superfamily. In efforts to determine merlin function several groups have discovered 34 merlin interacting proteins, including ezrin, radixin, moesin, CD44, layilin, paxillin, actin, N-WASP, betaII-spectrin, microtubules, TRBP, eIF3c, PIKE, NHERF, MAP, RalGDS, RhoGDI, EG1/magicin, HEI10, HRS, syntenin, caspr/paranodin, DCC, NGB, CRM1/exportin, SCHIP1, MYPT-1-PP1delta, RIbeta, PKA, PAK (three types), calpain and Drosophila expanded ...

Cell-cell adhesion is critical for the control of tissue organization and homeostasis. A family of proteins that regulate cell-cell adhesions is the FERM (4.1 protein, Ezrin, Radixin, Moesin) domain-containing proteins.One FERM domain protein, the non-receptor tyrosine phosphatase PTPN14, is mutated or deleted in several human cancers suggesting that it may be involved in tumor development and/or progression. Additionally, the loss of the FERM domain protein Merlin is associated with tumor development and metastasis.Both PTPN14 and Merlin have been shown to localize and possibly regulate adherens junction (AJ) functions. This work sought to determine if PTPN14 is required for the establishment of epithelial adhesion and polarity. Using mammalian epithelial cells as a model to test these hypotheses, we down-regulated the tyrosine phosphatase, PTPN14. Decreased levels of PTPN14 increased E-cadherin membrane movement at cell contacts, disrupting cell contacts and abolishing E-cadherin localization during

With support from a CDMRP FY09 Neurofibromatosis Research Program (NFRP) Investigator-Initiated Research Award, Dr. Duojia Pan and his research team at Johns Hopkins University sought to use Drosophila to identify downstream genetic pathways controlled by Merlin. Drosophila, commonly known as the fruit fly, has many evolutionarily conserved genes, including Merlin, that are strikingly similar to those in humans. Through this work, Pan and his team revealed multiple layers of evidence linking Merlin to the Hippo genetic signaling pathway, an important signaling pathway in cancer development. The Hippo pathway involves a signaling cascade of genes including Hippo (Hpo), Warts (Wts), and YAP. First, they discovered that YAP, which is elevated in many human cancers, genetically interacts with Merlin and the two genes function antagonistically to regulate mammalian tissue growth. Second, they showed that a protein complex, which consists of Kibra, Merlin, and Expanded, regulates Hippo signaling by ...

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Hello! I found this list by looking for Merlin Maxiprep protocol info. It was posted a *long* time ago (1994) but you can still find the protocol here: http://www.bio.net/bionet/mm/methods/1994-April/013440.html Ive been put in charge of making the Merlin solutions. When we first started using the assay, a couple different people had made them. Everything worked pretty well. We ran low on Merlin I, II, and IV, so I made up a more recently, and, horrors, the prep stopped working. D: The problem is that DNA is not precipitating during the spin-down after adding isopropanol to the filtered lysate. I think the problem must lie with either buffer I or buffer II; we are still using the old stock of buffer III, from when the assay was working. Ive already compared notes with the person who made up buffer I the first time (seems to be the same), re-made buffer I in case I got something horribly screwed up, and also tried a new solution of buffer II. Still no crude DNA pellet at the end of the spin. ...

(Yeah, spoilers - youve been warned.) I was surprised when A suggested going to see a horror movie. Usually, she refuses to watch anything that could conceivably be described as scary, while I seek out any and everything with a twisted, sinister heart held in the vice grip of some Cthulhu-esque monster. Having grown up…

Were setting the text of the label control to the Container.DataItem directly rather than using an Eval or Bind method. Thats because simple collections dont have properties that Eval and Bind can use, so you put the content of the data item directly in your output. This is the same with almost all bindable controls with simple collections, but is also one of those things the documentation just sort of assumes you already know… which is probably why I coded asp.net for about 4 years before I discovered this little trick.. On a postback or callback, there is no reliable way to get at the data items in their re-arranged state. If you put a button on the page to postback to the server and try to look at the items in the control, you will only be able to see them in the order they had been in when the control was first bound to the collection.. This means you cant just let the user make changes on the client and just submit the page back when they are done. The only reliable way to keep up ...

NF2 / Merlin兔多克隆抗体(ab47378)可与小鼠, 大鼠, 人样本反应并经WB, ELISA实验严格验证，被1篇文献引用并得到3个独立的用户反馈。所有产品均提供质保服务，中国75%以上现货。

Disclaimer: I own nothing, all rights belong to their respective owners.. Hope everyone had a lovely Christmas. This one takes place after 5x08.. Merlin knew that he was due in Arthurs chambers. He also knew that he had been due there for the last candle mark or so. He couldnt help it; his leg was slowing him down far more than he thought it would. He had let Gaius treat it once he had made sure that the king was no longer in any danger, but he hadnt told the old man precisely what had happened. He had just let Gaius think that he had taken a fall in the woods. Technically, it wasnt far from the truth: that had been how he had hurt the limb. But he just didnt want Gaius to know how close Morgana had come to actually succeeding removing the only barrier she had to killing the king. It worried Merlin that she already knew that he was part of the reason why Arthur survived every attack and that was without her knowing about his powers.. But he could still protect Gaius.. He knew that the old ...

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Rabbit polyclonal NF2 / Merlin antibody validated for IHC, ICC/IF and tested in Human. Immunogen corresponding to synthetic peptide

Nasze doswiadczenie w zajmowaniu sie medycyna alternetywna oraz medycyna niekonwecjonalna, to ponad 20 lat pracy z pacjentami w Polsce i za granica.

A Diablo II characters name must be unique to the Realm it is on. For example, a Realm can have only one Merlin character, but each Realm can have a Merlin character. ...

Live dashboard provides you with an overview of all view metrics in a compact format. Data updates every 15 seconds. Only those elements that contain a metric or graph report are supported. Aggregate graphs will not be included. You can get more graphs per page by using your browser zoom functionality. Please select the view you want to view. ...

Plasmid pXJ40-HA-Merlin I from Dr. Filippo Giancottis lab contains the insert Merlin I and is published in J Cell Biol. 2005 Oct 24. 171(2):361-71. This plasmid is available through Addgene.

Mutant KRAS (mut-KRAS) is present in 17-25% of all human cancers, where it plays a critical role in driving cancer cell growth and resistance to therapy. Despite numerous attempts, there is still no effective therapy for mut-KRAS tumors. Understanding the signaling mechanisms activated by mut-KRAS and finding agents to inhibit mut-KRAS signaling are important unmet needs in cancer therapy today. The recently completed BATTLE-1 clinical trial, a prospective, multi-arm, biopsy-mandated, biomarker-driven, clinical trial in advanced refractory non-small cell lung cancer (NSCLC), found that mut-KRAS did not accurately predict patient outcome (progression-free survival) to targeted intervention. This finding contradicted published evidence for such a relationship from colon cancer and some previous NSCLC studies. We explored more specifically the nature of the KRAS mutations, which are primarily found at codons 12 and 13, where different base substitutions lead to alternate amino acid (aa) ...

Neurofibromatosis type 2 (NF2) is a genetic syndrome that predisposes individuals to multiple benign tumors of the central and peripheral nervous systems, including vestibular schwannomas. Currently, there are no FDA approved drug therapies for NF2. Loss of function of merlin encoded by the NF2 tumor suppressor gene leads to activation of multiple mitogenic signaling cascades, including platelet-derived growth factor receptor (PDGFR) and SRC in Schwann cells. The goal of this study was to determine whether ponatinib, an FDA-approved ABL/SRC inhibitor, reduced proliferation and/or survival of merlin-deficient human Schwann cells (HSC). Merlin-deficient HSC had higher levels of phosphorylated PDGFRα/β, and SRC than merlin-expressing HSC. A similar phosphorylation pattern was observed in phospho-protein arrays of human vestibular schwannoma samples compared to normal HSC. Ponatinib reduced merlin-deficient HSC viability in a dose-dependent manner by decreasing phosphorylation of PDGFRα/β, AKT, ...

TY - JOUR. T1 - DNA Microarray analysis of immediate response to EGF treatment in rat schwannoma cells. AU - Oh, Min-Kyu. AU - Scoles, Daniels R.. AU - Pulst, Stefan M.. PY - 2005/9/1. Y1 - 2005/9/1. N2 - Epidermal growth factor (EGF) activates many intracellular effector molecules, which subsequently influence the expression levels of many genes involved in cell growth, apoptosis and signal transduction, etc. In this study, the early response of gene expressions due to EGF treatment was monitored using oligonucleotide DNA microarrays in rat schwannoma cell lines. An immunoblotting experiment showed the successful activation of EGF receptors and an effector protein, STAT5, due to EGF treatment. The microarray study showed that 35 genes were significantly induced and 2 were repressed within 60 min after the treatment. The list of induced genes included early growth response 1, suppressor of cytokine signaling 3, c-fos, interferon regulatory factor 1 and early growth response 2, etc. According to ...

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PREVIOUS PROJECTS. 2011-2013. 1. p53/mouse double minute 2homolog complex deregulation in Merlin-deficient tumours. This project involved investigation of the role of p53 deregulation inschwannoma development and targeting pathways involved in p53 degradation. Inthis project I was first author.. 2. Gas6/Axl-family receptors in schwannoma pathological proliferation, adhesionand survival. In this project we have investigated the role of Axl receptor inschwannoma pathological proliferation, cell-matrix adhesion and survival. Inthis project I was first and corresponding author.. 2010-2012. 1. The role of insulin-like growth factors (IGF1/2) signalling inMerlin-deficient human schwannomas. 2. Insulin-like growth factor-binding protein-1 (IGFBP-1) regulates human schwannomaproliferation, adhesion and survival. In these studies we have demonstrated that IGF1/2 and IGFBP-1 arereleased from schwannoma cells and IGF-I receptor overexpressed leading toincreased schwannoma proliferation and cell-matrix ...

André Merlin (15 November 1911 - 5 September 1960) was a French tennis player. Born in Brazzaville on 15 November 1911, he was the son of the Governor-General of French Equatorial Africa, Martial Henri Merlin. Merlin first represented the France Davis Cup team in the final of the 1933 tournament, against Great Britain at the Stade de Roland Garros. France, under The Four Musketeers, had won the previous six tournaments, but were an ageing side and brought in Merlin to counter the younger British players. Merlin lost the opening match to Bunny Austin in a one sided encounter and Henri Cochet was beaten by Fred Perry, but the French fought back to win the doubles then levelled the tie when Cochet overcame Austin in five sets. This meant it came down to Merlin in the fifth rubber, with his match against Perry to decide the title. Merlin won the first set, but Perry fought back to win in four and secure Britains first title since 1912. In the second set, Merlin had failed to capitalise on two set ...

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The Hippo signaling pathway was originally discovered in Drosophila melanogaster and has recently emerged as a potent regulator of cell proliferation and organ size (Badouel et al., 2009; Zhang et al., 2009b). Several components of the pathway act as tumor suppressors or as protooncogenes (Harvey and Tapon, 2007). Core components of the Hippo pathway include the upstream activator Merlin/Nf2 (Hamaratoglu et al., 2006), a gene that is mutated in tumors of nervous tissue (Trofatter et al., 1993; Ruttledge et al., 1994) and in renal cell carcinoma (Forbes et al., 2008; Morris and McClatchey, 2009; Dalgliesh et al., 2010), the Ser/Thr kinases MST1/2 (mammalian STE20 kinases 1 and 2) and Lats1/2 (large tumor suppressor 1 and 2), together with their coactivators WW45 and Mob. In the active state, Lats1/2 phosphorylates the transcriptional activators Yes-associated protein (YAP) and TAZ (transcriptional coactivator with PDZ-binding domain). This results in their cytoplasmic retention by binding to ...

talk. Merlin was aware of the sense of an overwhelming intellectual power and realized that he was talking to God. Woden asked Merlin, What can I give you? Merlin said, I did not call you here to give me a gift and Im not sure if Im ready to feel obligated in terms of my religion. Woden said, We are not talking about your religion, we are talking about my religion. My religion is gift-giving and if you dont take my gift it could go ill for both of us. Merlin then said that he would like a job, a good job. Woden said, It is done. Merlin asked Woden how he could learn the things he needed to know. Woden said that he would put those things in place for him. When they finished talking Merlin said good-bye to God and began to ponder the experience. Within days, out of nowhere, Merlin was offered a job by the State, and went to work immediately. Woden, of course, stayed nearby Merlin, asking him if he was to be Wodens man. By late 1976 Merlin had changed his name to Garman, for he was now ...

Weve talked about the Merlin Bird ID app before but Im bringing it back up because a new update allows it to do EXACTLY what weve always wanted. It can now ID a bird from a picture.. The Merlin Bird ID app is part of the Cornell Lab of Ornithology which seeks to identify and preserve natures feathered friends in North America. Most of us can identify your common robins, sparrows, gulls, etc but the ones that a bit rarer are more of a mystery to the untrained eye.. Now all you need to do is snap a clear picture of the bird and the app will compare it to the database. It uses information from the picture as well as location and seasonal data to tell you what you are looking at. Though not perfect, provided you get a good picture, the app has a 90% success rate. You can also upload pictures from better cameras, because getting close enough to a bird to capture something on your smartphone is pretty difficult. Merlin Bird ID is free.. ...

I write. I write a lot. A. LOT. There are times I am half blind with a sentence ricocheting off the walls of my stupid, cant be shut off to save my life, brain. I am miserable until I get it down on paper. Punch it up a bit. Usually cross out half of it. And then breathe. Relax. Only to do it all again..... But I just thought that was me. How I am. Not a writer....noooo...not me. Writers are.....writing people. People Who Write. REALLY write. Write things that matter. All grown up very important things. Not.....me. I am just a scribbler of sorts. And I was/am content with that....if its true, well then....a scribbler am I. Until the thought wormed its way in to my brain (the furtive sneaky bitch) that maybe...just maybe...that is writing. My style. My strange way. But....still writing. So here I am at the dance. Not sure I know any of the moves and the music is entirely mine. But.....only one way to find out. Would you care to join me ...

I write. I write a lot. A. LOT. There are times I am half blind with a sentence ricocheting off the walls of my stupid, cant be shut off to save my life, brain. I am miserable until I get it down on paper. Punch it up a bit. Usually cross out half of it. And then breathe. Relax. Only to do it all again..... But I just thought that was me. How I am. Not a writer....noooo...not me. Writers are.....writing people. People Who Write. REALLY write. Write things that matter. All grown up very important things. Not.....me. I am just a scribbler of sorts. And I was/am content with that....if its true, well then....a scribbler am I. Until the thought wormed its way in to my brain (the furtive sneaky bitch) that maybe...just maybe...that is writing. My style. My strange way. But....still writing. So here I am at the dance. Not sure I know any of the moves and the music is entirely mine. But.....only one way to find out. Would you care to join me ...

Andrew is 12. He loves wolves, reading, acting, fencing, and exploring the woods! He likes to ride his bike, run 5ks, play with cousins, and play video games. (Zelda is his current favorite). He plays the piano and the french horn. And he is a Boy Scout. He is also a pretty nice guy ...

The plan was to ascend the Rhyd-Ddu Path and return on the Ranger Track. This was the quieter side of the mountain so we were hoping for blissful peace throughout the journey. The Rhyd-Ddu path was easy to begin with; the incline was shallow and took us along a track that used to serve the old Bwlch Cwm Llan Slate Quarry before getting relatively steep and rocky as we headed towards Llechog Ridge. It was between these points that we were introduced to the snow; a dusting to begin with but quickly deepened until it hit my knee height (approximately 2ft) a short time later. As it happens the snow was particularly prominent along the ridge and without cramp-ons or an ice axe the route became serious. I havent had any experience with trekking in the snow and I suddenly became aware of the steep drops on either side of me and how close I was to the edge. It didnt help that I had no clue about the terrain below the snow, I could happen a guess but what if I got it wrong? Instead I opted to just take ...

Mommy recuperated somewhat from her c-section and felt human enough to wear real clothes on Thursday and stopped taking the heavy pain killers. Hooray! Then came down with mastitis that same night and went back on the heavy pain killers and also an antibiotic. (Ouch!) One of her dearest friends moved away that same day, so she cried off and on the whole night, tears of sadness and tears of pain. She feels human again tonight and prepared to be the Mommy of three tomorrow all on her own. Say a prayer for her! Shes a little more nervous then she would like to admit ...

I was into my fourth month in a locked ward, sitting in a lounge facing the entrance doors when I could have cried at the vision I saw walk through them. She was a friend, she was my lawyer but more than this - she was the Scottish equivalent of the District Attorney (Procurator Fiscal). Well known throughout the region she was a true Public figure. I swear I saw some of the staff go weak at the knees when they realised who she was - they had no idea why she was here - was it an investigation? She told them crisply and clearly but politely that she was here to see Rory Grant and they almost fell over one another in their haste to lead her toward me. I stood up and we hugged. She sat in the chair next to me and apologised for not visiting sooner - but shed been under the impression Id be getting out sooner rather than later and had fully expected to be visiting me at home by now. We talked for an hour or so before we smilingly parted company - she to her delightful house overlooking the ...

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Merlin awoke in the middle of the day from the sudden shot of searing pain that scorched its way across his back. His vision was muddled for a moment, but that just gave him the time he needed to gather his thoughts and try to figure out a course of action. By the time his gaze had sharpened and he had pushed himself up into a sitting position, Merlin had decided a few things.. First things first, he was dying. He had saved himself from splattering across the ground, but the hydra had still wounded him. His body felt stiff and weak, the poison working its way through his system. Since he had not been bitten - the fangs had scraped across his back and stomach, exposing flesh and gashing deeply, but nothing as bad as it could have been - there was less venom in his body. He was weak, and he guessed he only had a few days left to live, but that was still plenty of time to try and heal.. Next was realizing that the knights probably thought he was dead. He could feel the suns warmth tickling his ...

Machine Wash Cold with Like Colors, Non-chlorine bleach as needed, Tumble Dry Low or Hang Dry, No Iron The Merlin Magic Sleepsuit is the original swaddle transition product The Magic Sleepsuit is designed for back sleeping in the crib at the recommended room temperature for babies

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The Return of Merlin - Chopra, Deepak - The author of the million-copy best-sellerAgeless Body, Timeless Mindemerges as a powerful new force in fiction with a luminously written novel about the final act of the Arthurian legend playing out in modern England.The Return of Merlinis a brilliantly realized narrative that begins in Arthurian times and jumps boldly to our own 20th-century dark age of war, pollution, predation, and hatred--with a message of hope.

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Merlin MP3-Player Beta VersionMotivation: Nachdem meine E2 Boxen leider nicht in der Lage sind, MP3 Lieder von Anfang bis zum Ende abzuspielen (fehlt immer ein Stück) und die Pausen zwischen den Liedern unerträglich lang sind, so dass beispielsweise…

The PerkinElmer Merlin MicroSeal Injector Port Septa, 11 mm, Pkg 1 is a unique replacement septa employing a two-step sealing system and an advanced elastomer material.

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St. Jude Medical is releasing a new version of the Merlin.net remote monitoring system for patients with implanted cardiac devices. The system provides phy

A few more of my MacBreak segments from the Macworld Expo floor are now available for download: MacBreak 51 Macworld 2007: The Omni Group - (MOV) - 8:03 - Merlin chats with Ken Case from the Omni Group about their products and the application

How Cloud Foundry Container Runtime (CFCR) tackles Day 1 and Day 2 Operations for Production Kubernetes Cloud Foundry Container Runtime (CFCR), formerly known as Project KUBO is an open source projec

Is she really busty enough to need a sports bra? Hm. (Methinks youve seen Jay Naylors Trixie character, heh.). … Wait, would that even be kosher? We havent seen furclad characters before, have we? Then again Priscilla was working in the nude for a while and didnt get in too much trouble, and the crystal people arent harassed for it, so… merf?. ...

Stack Exchange network consists of 178 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers. Visit Stack Exchange ...

Last night, 60 Minutes carried a segment on the replacement of humans by machines -- like back in the mid-1800s with the so-called Industrial Revolution. Back then, back-breaking stoop-labor was supplanted by machines, but in the process, the men and women who performed that stoop-labor were kicked to the curb. With no source of income,…

Forex 3mm thickness is a versatile, lightweight plate of 3mm. The plate has a foamed, porous core and is suitable for indoor and (temporarily) outdoor usage. The material is widely used for Point of Sale operations and is easy to print and edit. Forex is excellent milling contour and thinner sheets can be bent slightly.

Today I was in a cyber mood again and I was waiting for so long to have a reason to buy that cool tentacles from [CX]. Being in SL for over 10 years, normally means you have a uncontrollable inventory - at least in my case. I do have so many things, even if I do not use them to much - or not at all - some of them have amazing memories to them and I simply cant delete them. I do sort and as well clean it out, but no matter what I cant make it below a certain number and even that number grows every year for some reason :p. That was why I did impose a rule on myself… Never buy something you do not have an initial idea for, or that you do not use in a reasonable time frame! Sometimes that is really hard as I see many things on events and in stores, that I do like immensely…. With that, I at least protect my inventory from exploding too much but, it still grows… just slower*giggles*. It did change my whole shopping habit a lot, as well it did change my view on some things. I used to buy ...

After a contributor suggests a string, the string gets a status of suggested. In order to transform them into approved strings (which will be used in WordPress), a Translation Editor needs to accept (or reject) those suggestions ...

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Hoy les dejo esta banda rusa de death metal lidereada por una mujer en la voz, como podran notar me gustan las bandas con vocal femenina, esta bastante potente la banda ...

Several people love to customize these wheels or stain them so that they remain as beautiful as the day they bought them. However, taking a spinning wheel...

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This week, Dan and Merlin continue their series on updates, talking about the evolution of electronic task management in their lives. Hint: _simplify, simplify_.

With Merv The Process of photo -synthesis And Merlin What do you mean nothing? So many things happen inside of you every day! Ugh, another day of doing

America is dead; the United States of America is officially history as of November 13th, Duke said matter-of-factly to Colonel Merlin Jones in a sid

In this application note, the capability of the ZEISS MERLIN for high-resolution imaging at low acceleration voltages and low beam currents is demonstrated.