Surface enhanced Raman spectroscopy (SERS) for the detection of intracellular constituents using gold nanoshells -ORCA
This study reports on current work involving the use of Surface Enhanced Raman Spectroscopy (SERS) for the intracellular detection of cell constituents in mouse fibroblast cells using gold nanoshells. Gold nanoshells were acquired from Nanospectra Biosciences that are based on a silica dielectric core and an outer gold shell layer. They have the unique property of a tunable surface plasmon resonance wavelength from the visible through the near infrared which allows control of the electromagnetic field strength on its surface. Hence gold nanoshells can serve as SERS substrates with plasmonic properties that are not aggregation dependent and thus can be expected to overcome the reproducibility problem that is generally associated with aggregation based colloidal metal nanoparticles. These results represent the first steps in the development of a nanoshell-based SERS probe to detect cell organelles and/or intracellular biochemicals with the goal of ultimately improving the ability to monitor ...
Stone Sealer S Waterproofing Spray | nanoShell Stone | Impregnation Protective Coatings at nanoShell Ltd
nanoShell Stone Protector S is a clear, solvent based ready-to-use coating that protects facades from physical and chemical damages. nanoShell Stone Protector S forms a permanent monomolecular layer inside the capillaries that make the pore surface water-
Macrophages loaded with gold nanoshells for photothermal ablation of g by Amani Riad Makkouk
The current median survival of patients with glioblastoma multiforme (GBM), the most common type of glioma, remains at 14.6 months despite multimodal treatments (surgery, radiotherapy and chemotherapy). This research aims to study the feasibility of photothermal ablation of glioma using gold nanoshells that are heated upon laser irradiation at their resonance wavelength. The novelty of our approach lies in improving nanoshell tumor delivery by loading them in macrophages, which are known to be recruited to gliomas via tumor-released chemoattractive agents. Ferumoxides, superparamagnetic iron oxide (SPIO) nanoparticles, are needed as an additional macrophage load in order to visualize macrophage accumulation in the tumor with magnetic resonance imaging (MRI) prior to laser irradiation. The feasibility of this approach was studied in an in vitro model of glioma spheroids with the use of continuous wave (CW) laser light for ablation.
The optimal loading of both murine and rat macrophages with Ferumoxides
Shell-thickness-dependent nonlinear optical properties of colloidal gold nanoshells - Journal of Materials Chemistry C (RSC...
Third-order nonlinear optical properties of gold nanoshells of different thickness were investigated over a broad wavelength range (530-1200 nm) by the Z-scan technique using femtosecond laser pulses. Nonlinear absorption of the nanoshells is dominated by strong absorption saturation phenomena. The reciproca
Optimization of nanoshell mixtures for solar applications
The plasmon resonance in metallic nanoshells can be used to efficiently harvest solar energy and convert it into thermal or electronic form. Possible applications include improved optical coupling into silicon photodiodes, solar water heaters, and photocatalysis. We use standard optimization algorithms to theoretically determine the best mixture of different nanoshell species ([core, shell] sizes) for two practical scenarios. We show that a mixture of nanoshell species [r1, r2] = [47, 58] nm and [r1, r2] = [28, 42] run in a 6:5 volume ratio is optimal for absorbing AM 1.5 sunlight when deposited on a silicon surface. Surprisingly, we find that a single particle species is very good for scattering AM 1.5 light on a glass surface, and that very little benefit is gained by mixing different shells. Assumptions and approximations made in the analysis are discussed ...
DiagNano™ Carboxyl Gold Nanoshells, 150 nm - CD Bioparticles
Use DiagNano™ Carboxyl Gold Nanoshells, 150 nm for fast, easy, and consistent DNA/RNA Purification, Antibody/Protein Purification, Cell Isolation.
OSA | Nanoshells for photothermal therapy: a Monte-Carlo based numerical study of their design tolerance
The optimization of the coated metallic nanoparticles and nanoshells is a current challenge for biological applications, especially for cancer photothermal therapy, considering both the continuous improvement of their fabrication and the increasing requirement of efficiency. The efficiency of the coupling between illumination with such nanostructures for burning purposes depends unevenly on their geometrical parameters (radius, thickness of the shell) and material parameters (permittivities which depend on the illumination wavelength). Through a Monte-Carlo method, we propose a numerical study of such nanodevice, to evaluate tolerances (or uncertainty) on these parameters, given a threshold of efficiency, to facilitate the design of nanoparticles. The results could help to focus on the relevant parameters of the engineering process for which the absorbed energy is the most dependant. The Monte-Carlo method confirms that the best burning efficiency are obtained for hollow nanospheres and exhibit ...
Initial Evaluation of the Safety of Nanoshell-Directed Photothermal Therapy in the Treatment of Prostate Disease<...
TY - JOUR. T1 - Initial Evaluation of the Safety of Nanoshell-Directed Photothermal Therapy in the Treatment of Prostate Disease. AU - Stern, Joshua M.. AU - Kibanov Solomonov, Viktor V.. AU - Sazykina, Elena. AU - Schwartz, Jon A.. AU - Gad, Shayne C.. AU - Goodrich, Glenn P.. PY - 2016/1/1. Y1 - 2016/1/1. N2 - To evaluate the clinical safety profile for the use of gold nanoshells in patients with human prostate cancer. This follows on the nonclinical safety assessment of the AuroShell particles reported previously. Twenty-two patients, with biopsy diagnosed prostate cancer, underwent nanoshell infusion and subsequent radical prostatectomy (RRP). Fifteen of these patients had prostates that were additionally irradiated by a single-fiber laser ablation in each prostate hemisphere prior to RRP. Patients in the study were assessed at 9 time points through 6 months postinfusion. Adverse events were recorded as reported by the patients and from clinical observation. Blood and urine samples were ...
Early tests find nanoshell therapy effective against br... ( HOUSTON -- (Feb. 1 2011) -- Rice Uni...)
...HOUSTON -- (Feb. 1 2011) -- Rice University bioengineers and physicia...The results of the new study are available online in the Journal of... This first round of in vivo animal tests suggests that photothermal t...Glioma is among the most aggressive and difficult-to-treat of all brai...,Early,tests,find,nanoshell,therapy,effective,against,brain,cancer,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
gold-dielectric-gold multilayer nanoshell surrounded by silver shell
Page contains details about gold-dielectric-gold multilayer nanoshell surrounded by silver shell . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Enhanced penetration of lipid functionalized nanoparticles in 3D cell by Christopher Gene England 1988
Nanoparticle-based systems can alleviate systemic toxicity via surface functionalization to promote tissue-specific targeting as well as passive targeting abilities [1], such as the enhanced permeation and retention effect (EPR) [2]. The transport of nanoparticles is limited in hypoxic tumor regions due to the typically impaired tumor vasculature. To enhance diffusion of nanotherapeutics within tumor tissue; functionalized citrate gold nanoparticles and silica gold nanoshells with surface modifications of phosphatidylcholine (PC) and high density lipoprotein (HDL) were synthesized. 3D cell cultures were used as a representative model of portions of hypoxic tissue in liver, lung, and pancreatic solid tumors. Our results indicate that two layered silica gold nanoshells (surface modifications of -thiol and PC) permit enhanced accumulate compared to PEGylated nanoparticles. The addition of HDL for the smaller, citrate gold nanoparticles as a third external layer provided enriched accumulation compared to
OSA | Concentration dependence of gold nanoshells on the enhancement of optical coherence tomography images: a quantitative...
James Chen Yong Kah,1 Tzu Hao Chow,2 Beng Koon Ng,2 Sirajudeen Gulam Razul,2 Malini Olivo,3,4,5 and Colin James Richard Sheppard1,* 1Division of Bioengineering, National University of Singapore, 7 Engineering Drive 1, Singapore 117574 2School of Electrical and Electronics Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798 3Division of Medical Sciences, National Cancer Centre Singapore, 11 Hospital Drive, Singapore 169610 4Singapore Bioimaging Consortium, Biomedical Sciences Institutes, 11 Biopolis Way, #02-02 Helios, Singapore 138667 5Department of Pharmacy, National University of Singapore, Blk S4, 18 Science Drive 4, Singapore 117543 *Corresponding author: [email protected] ...
thiol-terminated polyethylene glycol-coated mesoporous plasmonic Ag/Au alloy nanoshells
Page contains details about thiol-terminated polyethylene glycol-coated mesoporous plasmonic Ag/Au alloy nanoshells . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Synthetic immunogenic cell death mediated by intracellular delivery of STING agonist nanoshells enhances anti-cancer chemo...
Many favorable anti-cancer treatments owe their success to the induction immunogenic cell death (ICD) in cancer cells, which activates antigen presenting cells to prime anti-cancer adaptive immunity. We describe a strategy to synthetically induce ICD by delivering the agonist of stimulator of interferon genes (STING), 23′-cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) into tumor cells using hollow polymeric nanoshells. Following intracellular delivery of exogenous adjuvant, subsequent cytotoxic treatment creates immunogenic cellular debris, by a process herein termed synthetic immunogenic cell death (sICD). sICD is indiscriminate to the type of chemotherapeutics adopted for cancer treatment and enables co-localization of exogenously administered immunologic adjuvants and tumor antigens for enhanced antigen presentation and development of anticancer adaptive immunity. In three mouse tumor models with distinctive chemotherapeutic treatments, sICD enhances therapeutic efficacy ...
Clinicians | Nanospectra
The detection of a gold nanoparticle contrast agent is demonstrated using a photothermal modulation technique and phase sensitive optical coherence tomography (OCT). A focused beam from a laser diode at 808 nm is modulated at frequencies of 500 Hz-60 kHz while irradiating a solution containing nanoshells. Because the nanoshells are designed to have a high absorption coefficient at 808 nm, the laser beam induces small-scale localized temperature oscillations at the modulation frequency. These temperature oscillations result in optical path length changes that are detected by a phase-sensitive, swept source OCT system. The OCT system uses a double-buffered Fourier domain mode locked (FDML) laser operating at a center wavelength of 1315 nm and a sweep rate of 240 kHz. High contrast is observed between phantoms containing nanoshells and phantoms without nanoshells. This technique represents a new method for detecting gold nanoparticle contrast agents with excellent signal-to-noise performance at ...
Porous Phospholipid Nanoshells as Enzymes Delivery Agents
Diabetes is an epidemic in developed nations. Glucokinase (GK) is vital for glocose sensing, and is directly implicated in particular forms of diabetes. Studying pancreatic cells with altered GK activity would facilitate studies, but current methods for altering proteomes are lacking. Porous phospholipid nanoshells (PPNs) have traditionally been used as platforms for biologically derived nanosensors, though their biocompatibility and protease resistance well suits them as enzyme delivery agents. GK kinetics were investigated with an enzyme coupled reaction to determine the effect of encapsulation. It was determined that encapsulation increased the Hill coefficient by 5.8% and the S(0.5) by 1.8%. This small deviation may not be significant in physiological conditions. To observe a recovered function in cell lines upon reintroducing GK, constitutively expressed GK must first be knocked down with siRNA. As initial work toward an siRNA knockdown, immunoblotting conditions were optimized resulting in ...
Nanoshell sensors for cellular analysis - University of Arizona
DESCRIPTION (provided by applicant): The incidence of diabetes mellitus has reached epidemic proportions in the U.S. and will continue to increase rapidly across all age group and ethnicities for the foreseeable future. The resulting cost to society via health care, lost wages, etc. is staggering and the decreased quality of life is immeasurable. Type 2 diabetes is primarily manifest in two categories, insufficient insulin secretion and enhanced insulin resistance. In non-diabetics, increased serum glucose stimulates insulin secretion from the pancreatic ?-cell and decreases glucagon secretion from the pancreatic ?-cell. Glucose-stimulated insulin secretion is a complex process that is regulated via complex signaling pathways within the cell. The dynamics of cellular signaling, and corresponding insulin release, are critical to normal regulation of serum glucose, however, detection of many cellular signals is not possible due to a dearth of sensing technologies. Recent studies have rediscovered ...
Mitochondrial-targeted multifunctional mesoporous Au@Pt nanoparticles for dual-mode photodynamic and photothermal therapy of...
In the conventional non-invasive cancer treatments, such as photodynamic therapy (PDT) and photothermal therapy (PTT), light irradiation is precisely focused on tumors to induce apoptosis via the generation of reactive oxygen species (ROS) or localized heating. However, overconsumption of oxygen and restrict
2017 Nanoscale HOT Article Collection
Gadolinium-enriched polyaniline particles (GPAPs) for simultaneous diagnostic imaging and localized photothermal therapy of...
TY - JOUR. T1 - Gadolinium-enriched polyaniline particles (GPAPs) for simultaneous diagnostic imaging and localized photothermal therapy of epithelial cancer. AU - Lee, Taeksu. AU - Bang, Doyeon. AU - Park, Yeonji. AU - Kim, Sun Hee. AU - Choi, Jihye. AU - Park, Joseph. AU - Kim, Donghun. AU - Kim, Eunkyoung. AU - Suh, Jin Suck. AU - Huh, Yong Min. AU - Haam, Seungjoo. PY - 2014/1/1. Y1 - 2014/1/1. UR - http://www.scopus.com/inward/record.url?scp=84908029000&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84908029000&partnerID=8YFLogxK. U2 - 10.1002/adhm.201300636. DO - 10.1002/adhm.201300636. M3 - Article. C2 - 24550214. AN - SCOPUS:84908029000. VL - 3. SP - 1408. EP - 1414. JO - Advanced healthcare materials. JF - Advanced healthcare materials. SN - 2192-2640. IS - 9. ER - ...
Integration of photothermal therapy and synergistic chemotherapy by a porphyrin self-assembled micelle confers chemosensitivity...
Integration of photothermal therapy and synergistic chemotherapy by a porphyrin self-assembled micelle confers chemosensitivity in triplenegative breast cancer ...
Bionanotechnology Laboratories
January 8, 2018 - Nasim Ganjis paper Surface activity of poly(ethylene glycol)-coated silver nanoparticles in the presence of a lipid monolayer accepted in Langmuir.. August 15, 2017 - Bothun and lab embark on a new collaboration: NIEHS funds URI-led Superfund on fluorinated pollutants (poly- and per-fluoroalkylated substances).. May 9, 2017 - Akram Abbasi publishes her first article in Langmuir! Near Infrared Responsive Gold-Layersome Nanoshells.. April 25, 2017 - Matt Preiss collaborative work with Kitchens group (Clemson Univ) in J. Phys. Chem. B Hydrophobic Nanoparticles Modify the Thermal Release Behavior of Liposomes.. December 14, 2016 - Collaborative work with Nasim Ganji, Iftheker Khan, Aihong Xi, and Chris Bobba accepted to Langmuir Anionic and cationic silver nanoparticle binding restructures net-anionic PC/PG monolayers with saturated or unsaturated lipids.. July 14, 2016 - Yaser Kashcooli successfully defends his M.S. thesis NIR Light Activated Hollow Gold Nanoshell ...
Bladder Ca photothermal treatment yields encouraging results | Urology Times
Photothermal treatment with epidermal growth factor receptor-directed gold nanorods results in considerable antineoplastic activity in an early study of bladder cancer.
Publications | Functional Optical Imaging Lab
BACKGROUND AND OBJECTIVES: Gold nanoparticles (GNPs) such as gold nanoshells (GNSs) and gold nanorods (GNRs) have been explored in a number of in vitro and in vivo studies as imaging contrast and cancer therapy agents due to their highly desirable spectral and molecular properties. While the organ-level biodistribution of these particles has been reported previously, little is known about the cellular level or intra-organ biodistribution. The objective of this study was to demonstrate the use of intrinsic two-photon induced photoluminescence (TPIP) to study the cellular level biodistribution of GNPs. STUDY DESIGN/MATERIALS AND METHODS: Tumor xenografts were created in twenty-seven male nude mice (Swiss nu/nu) using HCT 116 cells (CCL-247, ATCC, human colorectal cancer cell line). GNSs and GNRs were systemically injected 24 hr. prior to tumor harvesting. A skin flap with the tumor was excised and sectioned as 8 $μ$m thick tissues for imaging GNPs under a custom-built multiphoton microscope. For ...
Publications | Reich Research Group - UC Santa Barbara
Huang, X., et al. (2017). Modularized Gold Nanocarriers for TAT-Mediated Delivery of siRNA. Small 13(8):1602473.. Targeted delivery of siRNA controlled by near-infrared light using hollow gold nanoshells has been demonstrated in cancer and stem cells models. Here, a universal surface module and several functionalization rules for the maximized delivery of short nucleic acids (here, siRNA) applicable for diverse gold nanocarriers are described. Streptavidin is devised as a handle to assemble biotinylated cell penetrating peptides (e.g., transactivating transcriptional activator (TAT)), as well as an insulator between the positive charge of TAT and the dense negative charge of RNA. However, direct linking of streptavidin to functional siRNA inhibits its silencing activity. The approach then involves the orthogonal assembly of two types of RNA strands: one with biotin modification for cell targeting and penetration (scaffold RNA); the other without biotin as functional RNA (i.e., siRNA). ...
2018 AACC
Booth Profile NanoComposix has developed high contrast probes for improving the sensitivity of lateral flow assays. 3 - 20X enhancement in sensitivity has been achieved with BioReady gold nanoshells compared to 40 nm gold particles. Whether you are looking for a colloidal gold commercial supplier or a development partner, our lateral flow team can assist with conjugate design, antibody selection, strip optimization and transfer to manufacturing. Stop by our booth to learn more about our service options.. ...
People in the news - Union College
Roman Yukilevich, assistant professor of biology, was a first co-author on Incompatibility and Competitive Exclusion of Genomic Segments Between Sibling Drosophila Species, accepted for publication in Plos Genetics. His research investigated the extent to which recently diverged species show genetic incompatibilities, such that genomic regions of one species are unable to be incorporated into the genome of the second species. The Journal of Applied Physics accepted a manuscript written by Rebecca Cortez, assistant professor of mechanical engineering, and colleagues at the Air Force Research Laboratories and Rice University. Electrical Conductivity of Cationized Ferritin Decorated Gold Nanoshells addresses the tuning of electrical properties for future generations of nanodimensional electronic circuits. The work was partially funded by National Science Foundation grants.. Stephen Romero, associate professor of psychology, has pledged to bicycle 200 miles in two consecutive days this summer to ...
Self-assembly of nanoparticles into biomimetic capsid-like nanoshells - Immunology
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
Now, Silica Nanobubbles for Targeted Drug Delivery ( Indian researchers at the Indian Instit...)
Health,Indian researchers at the Indian Institute of Technology (IIT) Chenna...The researchers have used the Core-shell system that comprises a...The possibility of delivering therapeutic molecules into cells and...They incorporated ciprofloxacin (a known antibiotic) molecules on ... A hollow nanobubble or nanoshell of silica (10-20 nm in diameter...,Now,,Silica,Nanobubbles,for,Targeted,Drug,Delivery,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Novel Multifunctional Nanoagent for Visual Chemo/Photothermal Therapy of Metastatic Lymph Nodes via Lymphatic Delivery |...
Breast cancer is one of the major diseases that threaten womens health. Lymph node (LN) metastasis is the most common metastatic path of breast cancer. Finding a simple, effective, and safe strategy to eliminate metastatic tumors in LNs is highly desired for clinical use. Carbon nanoparticles (CNs), as an LN tracer, have been widely used in the clinical setting. In addition, previous experiments have confirmed that CNs have good photoacoustic imaging and photothermal effects.
Fucoidan-coated CuS nanoparticles for chemo-and photothermal therapy against cancer | Oncotarget
https://doi.org/10.18632/oncotarget.23898 Bian Jang, Madhappan Santha Moorthy, Panchanathan Manivasagan, Li Xu, Kyeongeun Song, Kang Dae Lee, Minseok Kwak, Junghwan Oh, Jun-O Jin
Indo-Canadian Student Tweaks Photothermal Therapy for Effective Cancer Treatment
A Canadian high school student of Indian origin has improved an ineffective experimental cancer therapy pairing it with antibiotics and has earned accolades from a panel of eminent scientists.
Highly photosensitive phospholipid nanovesicles for near infrared light-triggered local anesthesia
we propose a highly photosensitive nanovesicle to achieve …, which consists of gold nanoparticles attached to mechanoresponsive liposomes
Light-triggered inactivation of enzymes with photothermal nanoheaters - Strathprints
Enzymes are crucial for the cell metabolism and in industrial applications such as the manufacture of biosensors and medicines. A method for inactivating enzymes non-invasively and on demand could reveal the role of a biocatalyst in a particular cell function and enable fine-tuning the therapeutic dose of enzyme-based drugs. In this manuscript we introduce a universal method for inactivating enzymes with light. It consists of attaching the enzymes to photothermal nanoheaters (gold nanorods). Upon irradiation with light resonant with their LSPR the plasmonic nanoparticles generate heat, which denatures the enzymes. The resulting increase in temperature is highest in the vicinity of the nanoheaters, which allows the localized inactivation of nanorod-bound enzymes without damaging other proteins or cell membranes present in the same solution. Here we demonstrate this concept by selectively turning off an enzyme cascade via the selective inactivation of one of its components. We also prove that the ...
Light-Triggered Genes Reveal the Hidden Workings of Memory | Gizmocrazed - Future Technology News
A future technology news site addressing latest advancements in artificial intelligence, virtual reality, robots, gadgets and mobile technology.
FLJ12894 | CDK-II inhibitor KN-62 increase T-cell proliferation and IL-2 expression
LbL self-assembly, which was 1st reported almost 25 years ago,3 utilizes a wide range of interactions of varied strength, including covalent bonds,4 metal-ligand coordination,5 and electrostatic attractions between oppositely charged polyelectrolytes. 6 Whereas molecular self-assembly was initially developed for depositing monolayer and multilayer films on planar substrates, electrostatic LbL self-assembly of polyelectrolytes has recently been shown to be a versatile strategy for the synthesis of core-shell nanostructures and nanoshells.7 In this work, we have prepared cancer-specific MFNPs by LbL self-assembly of polyelectrolytes and explored their applications in magnetic resonance (MR) and optical imaging. Scheme 1 illustrates our LbL self-assembly strategy for MFNPs which were synthesized starting from recently reported hybrid silica nanoparticles (NP0) containing a luminescent [Ru(bpy)3]Cl2 core and a paramagnetic monolayer coating of the Gd-(siloxylpropyl)diethylenetriamine tetraacetate ...
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Nanoshells could deliver more chemo with fewer side effects, using In vitro study verifying a method for remotely triggering release of cancer drugs
Development of ultrasound bioprobe for biological imaging | Science Advances
We report the development of an ultrasound bioprobe for in vitro molecular imaging. In this method, the phase of the scattered ultrasound wave is mapped to provide in vitro and intracellular imaging with nanometer-scale resolution under physiological conditions. We demonstrated the technique by successfully imaging a magnetic core in silica core shells and the stiffness image of intracellular fibers in endothelial cells that were stimulated with thrombin. The findings demonstrate a significant advancement in high-resolution ultrasound imaging of biological systems with acoustics under physiological conditions. These will open up various applications in biomedical and molecular imaging with subsurface resolution down to the nanometer scale. ...
BHU researchers use laser to remove blood clots - Science Chronicle
It is now possible to remove blood clots in a less risky way, thanks to research carried out by scientists at the Institute of Medical Sciences, Banaras Hindu University, Varanasi. A team led by Prof. Debabrata Dash from the Department of Biochemistry has for the first time used photothermal therapy using near-infrared (NIR) laser and…
Performance of methods for enrichment detection on co-e | Open-i
Performance of methods for enrichment detection on co-expression clusters. Out of the 1323 possible miRNA-cluster pairs, those with a correlation of r |
Smart MoS<sub>2</sub>/Fe<sub>3</sub>O<sub>4</sub> nanotheranostic for magnetically targeted photothermal therapy guided by...
TY - JOUR. T1 - Smart MoS2/Fe3O4 nanotheranostic for magnetically targeted photothermal therapy guided by magnetic resonance/photoacoustic imaging. AU - Yu, Jie. AU - Yin, Wenyan. AU - Zheng, Xiaopeng. AU - Tian, Gan. AU - Zhang, Xiao. AU - Bao, Tao. AU - Dong, Xinghua. AU - Wang, Zhongliang. AU - Gu, Zhanjun. AU - Ma, Xiaoyan. AU - Zhao, Yuliang. N1 - Publisher Copyright: © 2015 Ivyspring International Publisher. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.. PY - 2015. Y1 - 2015. N2 - The ability to selectively destroy cancer cells while sparing normal tissue is highly desirable during the cancer therapy. Here, magnetic targeted photothermal therapy was demonstrated by the integration of MoS2 (MS) flakes and Fe3O4 (IO) nanoparticles (NPs), where MoS2 converted near-infrared (NIR) light into heat and Fe3O4 NPs served as target moiety directed by external magnetic field to tumor site. The MoS2/Fe3O4 composite (MSIOs) functionalized by biocompatible polyethylene glycol (PEG) were ...
IP Profile: Melanin-based nanoshells for protection against electromagnetic radiation and for energy conversion
We established that melanin-based nanoshells can be used in the protection of human beings and machinery from electromagnetic radiation, in particular, from ionizing radiation, and in energy conversion such as minimizing the losses during energy transfer.
Congratulations to Dr. Demosthenes Morales for Successfully Defending on May 26th, 2017!!! | Reich Research Group - UC Santa...
Introduction: The utility of recombinant proteins as potential therapeutics and cellular tools are largely impeded by endosome entrapment and susceptibility of delivered proteins in lysositic environments and degradative enzymes. We describe a modular, light-activated nanocarrier that transports proteins into cells by receptor-mediated endocytosis, and deliver the cargo to the cytosol by light triggered endosomal escape in a timely manner. The delivery platform is based on plasmonic hollow gold nanoshells (HGN) assembled with a cargo linking layer based on nickel affinity capable of supporting virtually any soluble poly-histidine tagged proteins. Transport into endosomes is mediated by the fusion of cell-penetrating peptides onto the protein of interest or by presenting an orthogonal internalizing handle. Once internalized, cytosolic localization of the protein of interest is achieved by excitation of nanocarriers with pulsed near-infrared (NIR) light to trigger protein release and disruption of ...
Targeted intracellular delivery of proteins with spatial and temporal control<...
TY - JOUR. T1 - Targeted intracellular delivery of proteins with spatial and temporal control. AU - Morales, Demosthenes P.. AU - Braun, Gary B.. AU - Pallaoro, Alessia. AU - Chen, Renwei. AU - Huang, Xiao. AU - Zasadzinski, Joseph A.. AU - Reich, Norbert O.. N1 - Publisher Copyright: © 2014 American Chemical Society. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.. PY - 2015/2/2. Y1 - 2015/2/2. N2 - While a host of methods exist to deliver genetic materials or small molecules to cells, very few are available for protein delivery to the cytosol. We describe a modular, light-activated nanocarrier that transports proteins into cells by receptor-mediated endocytosis and delivers the cargo to the cytosol by light triggered endosomal escape. The platform is based on hollow gold nanoshells (HGN) with polyhistidine tagged proteins attached through an avidity-enhanced, nickel chelation linking layer; here, we used green fluorescent protein (GFP) as a model deliverable cargo. Endosomal ...
Shannon Luminous Materials, Inc.
Shannon carries an extensive line of fluorescent products such as lacquer, aerosol spray cans, water base acrylic paint, varnish coatings, pigments, invisible dyes, invisible inks, re-admission ink, plastisol, enamel, fingerprint powder, pencils, adhesive additives, crayons, chalks, and fluorescent metal marking ink to name a few.. Our Phosphorescent products based on Zinc Sulfide are available in lacquer, enamel, acrylic water base, varnish, adhesive tape, plastisol and pigments.. The Super Phosphorescent line is made with Strontium long after glow and are available in acrylic water base paint, adhesive tape, plastic board, pellets, pigments, lacquer, varnish, rope, fabric, and much more.. Black lights have been a Shannon specialty since 1939. Our ultraviolet product offering contains everything from minature portable to stage spot lights. We have a black light for every need.. We are here for you so please call us and get the personal attention that you deserve! ...
Let there be tissue
Near-infrared light has better tissue penetration that is useful when the sample becomes thick, he explained. In addition to enhanced penetration -- up to 10 cm deep -- the light can be focused tightly to specific areas. Irradiation with the light released the RNA molecules from the nanoshells in the sample and initiated gene-silencing activity, which knocked down green fluorescent protein genes in the cell cluster. The experiment also showed that the irradiated cells grew at the same rate as the untreated control sample; the treated cells showed unchanged viability after irradiation.. Of course, culturing tissues consisting of related but varying cell types is a far more complex process than knocking down a single gene.. Its a concert of orchestrated processes, said co-author and graduate student researcher Demosthenes Morales, describing the process by which human embryonic stem cells become specific tissues and organs. Things are being turned on and turned off. Perturbing one aspect of ...
Guiding light | Electro Optics
Standard optical fibre, the type now widely used in telecoms, is pretty simple in terms of design, consisting of a glass silica core surrounded by cladding material with a lower index of refraction.
headspace-volatiles-distilled-spirits-spme-gc | China-Mainland | Sigma-Aldrich
A flexible StableFlex core is an improvement over a more rigid fused silica core. In addition to being a less breakable fiber, the sorbent coating will partially bond to the StableFlex, resulting in a more stable coating. For the GC analysis, a VOCOL® capillary GC column was used. Its intermediate polar nature enables strong dispersive (van der Waals forces) analyte-stationary phase interactions, along with π-π, dipole-dipole, and dipole-induced dipole interactions. The thick 1.50 μm film is necessary to provide focusing and increased retention of volatile analytes.. Slight heat, agitation of the vial, stirring, and the additional of salt are common ways to help drive volatiles into the headspace, which will increase sensitivity. The autosampler was programmed with a simple extraction (40 °C for 15 minutes with agitation). Replicates with salt (sodium chloride) added at 15% (w/v), and without salt, were extracted and analyzed for the first sample (a Kentucky bourbon). Similar ...
Publications | Laser Dynamics Lab
Huang XH, El-Sayed IH, EL-Sayed MA, Grobmyer SR. Applications of gold nanorods for cancer imaging and photothermal therapy. In: Methods in Molecular Biology. Vol. 624. Methods in Molecular Biology. Springer; 2010. pp. 343-357. Available from: http://dx.doi.org/10.1007/978-1-60761-609-2_23 ...
Publications | Laser Dynamics Lab
Huang XH, El-Sayed IH, EL-Sayed MA, Grobmyer SR. Applications of gold nanorods for cancer imaging and photothermal therapy. In: Methods in Molecular Biology. Vol. 624. Methods in Molecular Biology. Springer; 2010. pp. 343-357. Available from: http://dx.doi.org/10.1007/978-1-60761-609-2_23 ...
DI-fusion In vitro digital cell image analysis of morphonuclear...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.