Abstract Poly lactic acid is a biodegradable, biocompatible, and non-toxic polymer, widely used in many pharmaceutical preparations such as controlled release formulations, parenteral preparations, surgical treatment applications, and tissue engineering. In this study, we prepared lipid-polymer hybrid nanoparticles for topical and site targeting delivery of Norfloxacin by emulsification solvent evaporation method (ESE). The design of experiment (DOE) was done by using software to optimize the result, and then a surface plot was generated to compare with the practical results. The surface morphology, particle size, zeta potential and composition of the lipid-polymer hybrid nanoparticles were characterized by SEM, TEM, AFM, and FTIR. The thermal behavior of the lipid-polymer hybrid nanoparticles was characterized by DSC and TGA. The prepared lipid-polymer hybrid nanoparticles of Norfloxacin exhibited an average particle size from 178.6 ± 3.7 nm to 220.8 ± 2.3 nm, and showed very narrow ...
Boron Carbide Nanoparticles,Freestanding Gallium Nitride Substrate,Gallium Nitride Powder,Molybdenum Disulfide Nanoparticle,Nano Hydroxylapatie Powder,Silicon Carbide SIC Nanoparticles,Silicon Nitride Nanoparticles,Titanium Nitride Nanoparticles,Titanium Carbide Nanoparticles,Tungsten Carbide Nanoparticles,Zirconium Carbide Nanoparticles,Zirconium Boride Nanoparticles,Aluminium Nanoparticles,Bismuth Nanoparticles,Carbon Nanoparticles,Cobalt Nanoparticles,Copper Nanoparticles,Gold Nanoparticles,Graphite Nanoparticles,Iron Nanoparticles,Indium Nanoparticles,Molybdenum Nanoparticles,Nickel Nanoparticles,Silicon Nanoparticles,Silver Nanoparticles,Tin Nanoparticles,Titanium Nanoparticles,Tungsten Nanoparticles,Znic Nanoparticles,Nano Metal Oxide,ATO Nanoparticles,Alpha Aluminum Oxide,Gamma Aluminum Oxide,Nano CeO2,Nano CuO,Nano Cu2O,Nano Indium Oxide,Nano Indium Tin Oxide,Nano Lanthanum Oxide,Nano MgO,Nano Nickel Oxide,Hydrophobic Nano SiO2,Nano SiO2,Nano SnO2,Nano TiO2,Nano ZnO,Nano ZrO2 Copyright ...
Boron Carbide Nanoparticles,Freestanding Gallium Nitride Substrate,Gallium Nitride Powder,Molybdenum Disulfide Nanoparticle,Nano Hydroxylapatie Powder,Silicon Carbide SIC Nanoparticles,Silicon Nitride Nanoparticles,Titanium Nitride Nanoparticles,Titanium Carbide Nanoparticles,Tungsten Carbide Nanoparticles,Zirconium Carbide Nanoparticles,Zirconium Boride Nanoparticles,Aluminium Nanoparticles,Bismuth Nanoparticles,Carbon Nanoparticles,Cobalt Nanoparticles,Copper Nanoparticles,Gold Nanoparticles,Graphite Nanoparticles,Iron Nanoparticles,Indium Nanoparticles,Molybdenum Nanoparticles,Nickel Nanoparticles,Silicon Nanoparticles,Silver Nanoparticles,Tin Nanoparticles,Titanium Nanoparticles,Tungsten Nanoparticles,Znic Nanoparticles,Nano Metal Oxide,ATO Nanoparticles,Alpha Aluminum Oxide,Gamma Aluminum Oxide,Nano CeO2,Nano CuO,Nano Cu2O,Nano Indium Oxide,Nano Indium Tin Oxide,Nano Lanthanum Oxide,Nano MgO,Nano Nickel Oxide,Hydrophobic Nano SiO2,Nano SiO2,Nano SnO2,Nano TiO2,Nano ZnO,Nano ZrO2 Copyright ...
Lipid-polymer hybrid nanoparticles (LPHNPs) are next-generation core-shell nanostructures, conceptually derived from both liposome and polymeric nanoparticles (NPs), where a polymer core remains enveloped by a lipid layer. Although they have garnered significant interest, they remain not yet widely exploited or ubiquitous. Recently, a fundamental transformation has occurred in the preparation of LPHNPs, characterized by a transition from a two-step to a one-step strategy, involving synchronous self-assembly of polymers and lipids. Owing to its two-in-one structure, this approach is of particular interest as a combinatorial drug delivery platform in oncology. In particular, the outer surface can be decorated in multifarious ways for active targeting of anticancer therapy, delivery of DNA or RNA materials, and use as a diagnostic imaging agent. This review will provide an update on recent key advancements in design, synthesis, and bioactivity evaluation as well as discussion of future clinical
OBJECTIVE Docetaxel (DTX) remains the only effective drug for prolonging survival and improving quality of life of metastatic castration-resistant prostate cancer (mCRPC) patients. Combination anticancer therapy encapsulating DTX and another extract of traditional Chinese medicine is one nano-sized drug delivery system promising to generate synergistic anticancer effects, to maximize the treatment effect, and to overcome multi-drug resistance. The purpose of this study is to construct lipid-polymer hybrid nanoparticles (LPNs) as nanomedicine for co-encapsulation of DTX and curcumin (CUR). METHODS DTX and CUR co-encapsulated LPNs (DTX-CUR-LPNs) were constructed. DTX-CUR-LPNs were evaluated in terms of particles size, zeta potential, drug encapsulation, and drug delivery. The cytotoxicity of the LPNs was evaluated on PC-3 human prostate carcinoma cells (PC3 cells) by MTT assays. In vivo anti-tumor effects were observed on the PC3 tumor xenografts in mice. RESULTS The particle size of DTX-CUR-LPNs was
Page contains details about fluorescein isothiocyanate-loaded targeted lipid-polymer hybrid nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Page contains details about small interfering RNA against actin-loaded lipid-polymer hybrid nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Core-shell nanostructures are emerging as more important materials than alloy nanostructures and have much more interesting potential applications in various fields. In this work, we demonstrated the fast and facile synthesis of core-shell nanoparticles consisting of Pt thin layer as the shell and Ni nanoparticles as the cores. The described method herein is suitable for large-scale and low-cost production of the core-shell nanoparticles. X-ray diffraction, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy techniques were used to investigate the physicochemical characterizations. Importantly, the catalytic activity of [email protected] core-shell nanoparticles was probed to develop electrocatalyst in direct ethanol fuel cells (DEFCs). This electrocatalyst was applied to ethanol oxidation reaction for first time. Thus, the electrocatalytic activity of the [email protected] core-shell nanoparticles towards ethanol oxidation reaction has been investigated by cyclic voltammetry
The present invention is directed to cationic nanoparticles, methods to make them, and the use of compositions containing said nanoparticles in personal care compositions or formulations. The nanoparticles are useful in personal care applications and impart antimicrobial properties to home and personal care products containing them. These cationic nanoparticles also contribute useful conditioning properties to hair-care and skin-care products.
TY - JOUR. T1 - Cationic surface modification of PLG nanoparticles offers sustained gene delivery to pulmonary epithelial cells. AU - Baoum, Abdulgader. AU - Dhillon, Navneet. AU - Buch, Shilpa J. AU - Berkland, Cory. PY - 2010/1/1. Y1 - 2010/1/1. N2 - Biodegradable polymeric nanoparticles are currently being explored as a nonviral gene delivery system; however, many obstacles impede the translation of these nanomaterials. For example, nanoparticles delivered systemically are inherently prone to adsorbing serum proteins and agglomerating as a result of their large surface/volume ratio. What is desired is a simple procedure to prepare nanoparticles that may be delivered locally and exhibit minimal toxicity while improving entry into cells for effectively delivering DNA. The objective of this study was to optimize the formulation of poly(D,L-lactide-co-glycolide) (PLG) nanoparticles for gene delivery performance to a model of the pulmonary epithelium. Using a simple solvent diffusion technique, ...
Background: Accurate tumor localization is critical to performing laparoscopic colectomy which is lack of tactile sensation. The purpose of this study was to evaluate the feasibility and safety of using carbon nanoparticles to localize non-palpable tumor for laparoscopic colectomy, compared with intra-operative colonoscopy.. Methods: A prospective study was performed between July 2012 and September 2015. Inclusion criteria included T1-3 colon cancer, big adenoma or polyp unsuitable for endoscopic resection, multiple colorectal tumors, and cancer complete or partial response after neoadjuvant therapy. Exclusion criteria included T4 colon cancer, planned local excision, previous abdominal surgery, and emergency case with bleeding or obstruction or perforation. Sixty patients were enrolled in this study and divided into carbon nanoparticles group (30 cases) and intra-operative colonoscopy group (30 cases). One milliliter carbon nanoparticles suspension, which is approved by Chinese Food and Drug ...
Faceted colloidal nanoparticles are currently of immense interest due to their unique electronic, optical, and catalytic properties. However, continuous flow synthesis that enables rapid formation of faceted nanoparticles of single or multi-elemental composition is not trivial. We present a continuous flow synthesis route for the synthesis of uniformly sized Pd nanocubes and PdPt core-shell nanoparticles in a single-phase microfluidic reactor, which enables rapid formation of shaped nanoparticles with a reaction time of 3 min. The PdPt core-shell nanoparticles feature a dendritic, high surface area with the Pt shell covering the Pd core, as verified using high-resolution scanning transmission electron microscopy and energy dispersive X-ray spectroscopy. The Pd nanocubes and PdPt core-shell particles are catalytically tested during NO2 reduction in the presence of H2 in a flow pocket reactor. The Pd nanocubes exhibited low-temperature activity (i.e., <136 °C) and poor selectivity performance toward
Nanoparticle size controls their ability to move through the body. Smaller nanoparticles move through the body without getting stuck in tight spaces. They also are easier for cells to swallow, which is important because nanoparticles need to release their medicine inside cells to work. Smaller nanoparticles can also let go of medicine faster.. It might seem like a good idea to make the smallest nanoparticles possible, but there is a size limit that cant be crossed. If the nanoparticles are too small, they will clump together. Clumpy nanoparticles are no longer small enough to work properly. Thats why its important to stay within a certain size range when designing nanoparticles.. While their small size allows nanoparticles to go anywhere in the body, they still have to find their target organs or cell types. Scientists can program nanoparticles to find cell types from different organs, systems, or even tumors because each type of cell has different proteins on its surface. This is the cells ...
Nanoparticles are considered a primary vehicle for targeted therapies because they can pass biological barriers, enter and distribute in cells by energy-dependent pathways1-3. Until now, most studies have shown that nanoparticle properties, such as size4-6 and surface7,8, can affect how cells internalise nanoparticles. Here we show that the different phases of cell growth, which constitute the cell cycle, can also influence nanoparticle uptake. Although cells in different cell cycle phases internalised nanoparticles with similar rates, after 24 hours of uptake the concentration of nanoparticles in the cells is ranked according to the different cell cycle phases: G2/M , S , G0/G1. Nanoparticles were not exported from cells but the internalised nanoparticle concentration is split when the cell divides. Our results suggest that future studies on nanoparticle uptake should consider the cell cycle because in a cell population, the internalised nanoparticle dose in each cell varies as the cell cycles ...
Makefield Therapeutics Licenses Patent Rights Covering Its Hybrid Nanoparticle Delivery Platform From Albert Einstein College of Medicine
PCS and AFM analyses were carried out in order to characterize size and size distribution, surface properties, and shape of nanoparticles. The analyses showed that the produced nanoparticles have almost spherical shape, and they are formed with desirable surface morphology (they have very smooth surface). In addition, it was turned out that egg albumin nanoparticles had a mean size of less than 100 nm. The simple coacervation method was considered an appropriate method for the production of this type of nanoparticles. Therefore, egg albumin nanoparticles can be considered very good candidates to be used as drug and food nano-carriers ...
0153] In some embodiments, subject 2DG-functionalized polyoxalate nanoparticles exhibit differential metabolic uptake into a particular mammalian cell and/or tissue. In some embodiments, subject 2DG-functionalized polyoxalate nanoparticles exhibit differential metabolic uptake into a diseased mammalian fissile, e.g., subject 2DG-functionalized polyoxalate nanoparticles exhibit an at least about 1%, at least about 5%, at least about 10%, at least about 25%, at least about 50%, at least about 100% (or 2-fold), at least about 2.5-fold, at least about 5-fold, at least about 10-fold, at least about 15-fold, at least about 20-fold, at least about 50-fold, or at least about 100-fold, or more, greater metabolic uptake into the diseased tissue, compared to the metabolic uptake of the 2DG-functionalized polyoxalate nanoparticles into a non-diseased tissue, e.g., a non-diseased tissue of the same tissue type. For example, in some embodiments, subject 2DG-functionalized polyoxalate nanoparticles exhibit ...
Through a process of translocation across biological barriers, nanoparticles can reach and deposit in secondary target organs where they may induce adverse biological reactions. Therefore, a correct assessment of nanoparticle-induced adverse effects should take into account the different aspects of toxicokinetics and tissues that may be targeted by nanoparticles. For this reason, a comprehensive evaluation of renal nanotoxicity is urgently needed as kidneys are particularly susceptible to xenobiotics and renal excretion is an expected and possible elimination route of nanoparticles in living organisms. On one hand, summarizing the findings of in vitro and in vivo studies that have investigated the adverse effects of nanoparticles on the kidney, this review intends to provide a thorough insight into the nephrotoxicity of these substances. The evaluation of the in vitro studies revealed that different types of nanoparticles (carbon, metal and/or silica nanoparticles) are able to exert significant ...
TY - JOUR. T1 - Protein Nanoparticle Formation Using a Circularly Permuted α-Helix-Rich Trimeric Protein. AU - Kawakami, Norifumi. AU - Kondo, Hiroki. AU - Muramatsu, Masayuki. AU - Miyamoto, Kenji. PY - 2017/2/15. Y1 - 2017/2/15. N2 - We here report the production of highly spherical protein nanoparticles based on the domain-swapping oligomerization of a circularly permuted trimeric protein, major histocompatibility complex (MHC) class II associated chaperonin. The size distribution of the nanoparticles can be adjusted to between 40 and 100 nm in diameter, and thus, these particles are suitable as drug carriers following purification under basic conditions. Our approach involves no harsh treatments and could provide an alternative approach for protein nanoparticle formation.. AB - We here report the production of highly spherical protein nanoparticles based on the domain-swapping oligomerization of a circularly permuted trimeric protein, major histocompatibility complex (MHC) class II ...
The development of eco-friendly technologies in material synthesis is of considerable importance to expand their biological applications. Nowadays, a variety of inorganic nanoparticles with well-defined chemical composition, size, and morphology have been synthesized by using different microorganisms, and their applications in many cutting-edge technological areas have been explored. This paper highlights the recent developments of the biosynthesis of inorganic nanoparticles including metallic nanoparticles, oxide nanoparticles, sulfide nanoparticles, and other typical nanoparticles. Different formation mechanisms of these nanoparticles will be discussed as well. The conditions to control the size/shape and stability of particles are summarized. The applications of these biosynthesized nanoparticles in a wide spectrum of potential areas are presented including targeted drug delivery, cancer treatment, gene therapy and DNA analysis, antibacterial agents, biosensors, enhancing reaction rates, separation
The multi-kinase inhibitor sorafenib (tradename Nexavar®, Bayer) has been recently approved by the FDA for the treatment of non-resectable hepatocarcinoma and advanced renal carcinoma. Despite its proven survival benefit, sorafenib can lead to important side effects. The aim of this study is the development of a magnetic nanovector able to efficiently and selectively deliver sorafenib toward cancer lesions thanks to a physical guidance mediated by magnetic nanoparticles. Sorafenib and superparamagnetic iron oxide nanoparticles are encapsulated in solid lipid nanoparticles (SLNs), extensively characterized and in vitro tested on the hepatocellular carcinoma cell line HepG2. Obtained results suggest the possibility to prepare stable SLNs able to destroy HepG2 cancer cells through sorafenib cytotoxic effect, and to enhance this effect in a desired area thanks to the magnetically-driven accumulation of the drug.
The health risk and cytotoxic effects of nanoparticles are almost unknown. Consequently, we have launched an interdisciplinary research program to systematically investigate the toxicity of nanoparticles. An initial observation prompted us to hypothesize that SiO2 nanoparticles can traverse the plasma membranes of cells through the endocytosis mechanism and thereby exert their cellular and cytotoxic effects on cells. To test this hypothesis, we investigated the effects of silicon dioxide nanoparticles on two human brain tumor cell lines (SK-N-SH, a neuroblastoma line and U87, an astrocytoma line) employing light microscopy, lactate dehydrogenase release into the culture medium (an indicator of cell damage and necrosis) and MTT assay (an indicator of cell survival). Our results indicate exposure to SiO2 nanoparticles led to cytotoxic damage (as indicated by LDH release) and decreases in cell survival (as determined by the MTT assay) in SK-N-SH and U87 cells in a dose-related manner, their effect being
TY - JOUR. T1 - Development of solid lipid nanoparticles for enhanced solubility of poorly soluble drugs. AU - Potta, Sriharsha Gupta. AU - Minemi, Sriharsha. AU - Nukala, Ravi Kumar. AU - Peinado, Chairmane. AU - Lamprou, Dimitrios A.. AU - Urquhart, Andrew. AU - Douroumis, D.. PY - 2010/12. Y1 - 2010/12. N2 - Cyclosporine (CyA) solid lipid nanoparticles were prepared by using a solvent free high pressure homogenization process. CyA was incorporated into SLNs that consisted of stearic acid, trilaurin or tripalmitin lipid solid cores in order to enhance drug solubility. The process was conducted by varying lipid compositions, drug initial loading and applied homogenization pressure. The processing temperatures were above the lipid melting points for all formulations. The empty and CyA loaded SLN particles made were characterized for particle size stability over six months. Atomic force microscopy (AFM) and photon correlation spectroscopy (PCS) showed particle sizes ranging from 112-177 nm for ...
PhD Project - Nanoparticle delivery of antibiotics for treatment of pulmonary infection at Queens University Belfast, listed on FindAPhD.com
We develop second-harmonic nanoparticles as the contrast agents for cell imaging. Second-harmonic nanoparticles show promise as cell imaging probes due to their non-bleaching, non-blinking, and coherent signal. Nanoparticles of noncentrosymmetric crystal structures have high second-harmonic generation (SHG) efficiency and provide high contrast in a generally non-structured cell environment. Here, we use barium titanate (BaTiO3) nanoparticles with tetragonal crystal structure as imaging probes. Cytotoxicity tests performed on BaTiO3 nanoparticles with mammalian cells did not result in toxic effects. Specifically we observed no change in the cell metabolism after 24 hours incubation of the cells with high concentration of BaTiO3 nanoparticles. We demonstrate two methods of cell labeling with BaTiO 3 nanoparticles for imaging. One is non-specific labeling via endocytosis of the cells, which results in a great number of the nanoparticles randomly distributed inside the cells. The other is specific ...
Water-soluble cupric oxide nanoparticles are fabricated via a quick-precipitation method and used as peroxidase mimetics for ultrasensitive detection of hydrogen peroxide and glucose. The water-soluble CuO nanoparticles show much higher catalytic activity than that of commercial CuO nanoparticles due to their higher affinity to hydrogen peroxide. In addition, the as-prepared CuO nanoparticles are stable over a wide range of pH and temperature. This excellent stability in the form of aqueous colloidal suspensions makes the application of the water-soluble CuO nanoparticles easier in aqueous systems. A colorimetric assay for hydrogen peroxide and glucose has been established based on the catalytic oxidation of phenol coupled with 4-amino-atipyrine by the action of hydrogen peroxide. This analytical platform not only confirms the intrinsic peroxidase-like activity of the water-soluble cupric oxide nanoparticles, but also shows its great potential applications in environmental chemistry, biotechnology and
Nanomaterials have become a popular topic of research over the years because of their many important applications. It can be a challenge to stabilize the particles at a nanometer size, while having control over their surface features. Copper nanoparticles were synthesized photochemically using a photogenerated radical allowing spatial and temporal control over their formation. The synthesis was affected by the stabilizers used, which changed the size, dispersity, rate of formation, and oxidation rate. Copper nanoparticles suffer from their fast oxidation in air, so copper-silver bimetallic nanoparticles were synthesized in attempts to overcome the oxidation of copper nanoparticles. Bimetallic nanoparticles were synthesized, but preventing the oxidation of the copper nanoparticles proved difficult. One important application of nanoparticles that was explored here is in catalyzing organic reactions. Because of the fast oxidation of copper nanoparticles, silver nanoparticles were synthesized ...
We have previously shown that gadolinium oxide (Gd2O3) nanoparticles are promising candidates to be used as contrast agents in magnetic resonance (MR) imaging applications. In this study, these nanoparticles were investigated in a cellular system, as possible probes for visualization and targeting intended for bioimaging applications. We evaluated the impact of the presence of Gd2O3 nanoparticles on the production of reactive oxygen species (ROS) from human neutrophils, by means of luminol-dependent chemiluminescence. Three sets of Gd2O3 nanoparticles were studied, i.e. as synthesized, dialyzed and both PEG-functionalized and dialyzed Gd2O3 nanoparticles. In addition, neutrophil morphology was evaluated by fluorescent staining of the actin cytoskeleton and fluorescence microscopy. We show that surface modification of these nanoparticles with polyethylene glycol (PEG) is essential in order to increase their biocompatibility. We observed that the as synthesized nanoparticles markedly decreased the ...
TY - CHAP. T1 - Engineering well-characterized PEG-coated nanoparticles for elucidating biological barriers to drug delivery. AU - Yang,Qi. AU - Lai,Samuel K.. PY - 2017. Y1 - 2017. N2 - Poly(ethylene glycol) (PEG) coatings can substantially reduce nanoparticle uptake and clearance by immune cells as well as nonspecific interactions with the biological environment, thus potentially improving nanoparticle circulation times and biodistribution in target tissues such as tumors. Naturally, the "stealth" properties of PEG coatings are critically dependent on the density and conformation of surface PEG chains. However, there are significant technical hurdles to both generating sufficiently dense PEG coatings on nanoparticles and precisely characterizing their PEG grafting densities. Here, we describe methods for preparing PEGylated polymeric nanoparticles with precisely tunable PEG coatings without the use of organic solvents, quantifying PEGylation efficiency and density using a standard fluorescence ...
1. Chertok B, Webber MJ, Succi MD. et al. Drug delivery interfaces in the 21st century: From science fiction ideas to viable technologies. Mol Pharm. 2013;10:3531-43 2. Hubbell JA, Langer R. Translating materials design to the clinic. Nat Mater. 2013;12:963-6 3. Hu C-MJ, Fang RH, Luk BT. et al. Polymeric nanotherapeutics: clinical development and advances in stealth functionalization strategies. Nanoscale. 2013;6:65-75 4. Wicki A, Witzigmann D, Balasubramanian V. et al. Nanomedicine in cancer therapy: Challenges, opportunities, and clinical applications. J Control Release. 2015;200:138-157 5. Tan SW, Li X, Guo Y. et al. Lipid-enveloped hybrid nanoparticles for drug delivery. Nanoscale. 2013;5:860-72 6. Bamrungsap S, Zhao ZL, Chen T. et al. Nanotechnology in therapeutics: a focus on nanoparticles as a drug delivery system. Nanomedicine. 2012;7:1253-71 7. Al-Jamal WT, Kostarelos K. Liposomes: from aclinically established drug delivery system to a nanoparticle platform for theranostic nanomedicine. ...
Difference between revisions of "Topic4-Differentially Charged Hollow Core/Shell Lipid-Polymer-Lipid Hybrid Nanoparticles for Small Interfering RNA Delivery" ...
There are different applications of biocompatible magnetic nanoparticles (MNP) in biomedical technologies. The MNP can be applied to cell separation, immunoassay, magnetic resonance imaging (MRI), drug and gene delivery, minimally invasive surgery, radionuclide therapy, hyperthermia and artificial muscle applications (see [1] for example). Most of these applications require superparamagnetic state of MNP. However, nanoparticles agglomerate very easily. By this reason, the production methods of superparamagnetic nanoparticles (SPNP) are being developed. Those nanoparticles should be weakly interactive and as a result incapable to stick together.. One of the different ways to avoiding MNP agglomeration is the production of composite particles of the core-shell type [2]. The particle core made of iron oxide is superparamagnetic, and the polymeric shell does not allow them to agglomerate. The polymeric shell serves also to functionalize nanoparticles for specific applications [1, 2]. The diameter of ...
BackgroundIn recent years, due to their small size and increased surface-to-volume ratio, the use of nanoparticles in diagnosis, drug delivery, and treatment has received much attention. The most important problem in chemotherapy is the limited access to the central part of the tumor due to its low blood flow. The aim of this study was to investigate the toxicity of polydopamine-coated and uncoated  nanoparticles on melanoma B16-F10 cells.Methods nanoparticles were co-precipitated and coated with dopamine. Then, the effect of cytotoxicity of the coated and uncoated nanoparticles on the B16-F10 cells was studied using MTT assay.Findings nanoparticles were toxic to B16-F10 at the concentration of 450 μg/ml and duration of 72 hours. Coated nanoparticles had no toxicity at any concentration at the duration of 48 and 72 hours.ConclusionThe cytotoxic properties of uncoated nanoparticles were significantly higher than the coated nanoparticles. Polydopamine coating shell can reduce the
Exposure to respirable ultrafine particles (2.5 μM) can adversely affect human health and have been implicated with episodes of increased respiratory diseases such as asthma and allergies; the same is predicted for nanoparticles, which can reach deeper regions of the lung. The objective of this investigation was to examine the effects of carbon nanoparticles on alveolar macrophages. Macrophages (MΦ) function as the first line of defense against invading pathogens and are likely to be amongst the first cells affected by nanoparticulates. We focused on two manufactured nanoparticles: multiwalled carbon nanotube aggregates and black-carbon. The two were tested against MΦ in a chronic contact model. We hypothesized that exposure to nanoparticles would decrease MΦ ability to effectively respond to immunological challenge. Production of interleukin (IL)-12 and nitric oxide (NO), as well as phagocytosis, and MΦ activation were examined in response to lipopolysaccharide (LPS) following a 144 hr ...
TY - JOUR. T1 - Everolimus Nanoformulation in Biological Nanoparticles Increases Drug Responsiveness in Resistant and Low-Responsive Breast Cancer Cell Lines. AU - Bonizzi, Arianna. AU - Truffi, Marta. AU - Sevieri, Marta. AU - Allevi, Raffaele. AU - Sitia, Leopoldo. AU - Ottria, Roberta. AU - Sorrentino, Luca. AU - Sottani, Cristina. AU - Negri, Sara. AU - Grignani, Elena. AU - Mazzucchelli, Serena. AU - Corsi, Fabio. PY - 2019/8/2. Y1 - 2019/8/2. N2 - Everolimus (Eve) is an FDA approved drug that inhibits mammalian target of rapamycin (mTOR). It is employed in breast cancer treatment even if its responsiveness is controversial. In an attempt to increase Eve effectiveness, we have developed a novel Eve nanoformulation exploiting H-ferritin nanocages (HEve) to improve its subcellular delivery. We took advantage of the natural tumor targeting of H-Ferritin, which is mediated by the transferrin receptor-1 (TfR1). Breast cancer cells overexpressing TfR-1 were successfully recognized by H-Ferritin, ...
Researchers at Wake Forest Baptist Medical Center have modified electrically-conductive polymers, commonly used in solar energy applications, to develop revolutionary polymer nanoparticles (PNs) for a medical application. When the nanoparticles are exposed to infrared light, they generate heat that can be used to kill colorectal cancer cells.
Porous hyperbranched conjugated polymer nanoparticles with an average particle size of 20-60 nm and a specific surface area of 225 m2 g−1 have been prepared through Suzuki polymerization in a miniemulsion, which could be stably dispersed in common organic solvents after complete removal of surfactants. Furth
Oral exposure to nanoparticles incorporated in foods is increasingly common. However, the effects of these nanoparticles when ingested are still unknown. Thus, this investigation tested the hypothesis that nanoparticle toxicity is dependent on particle size, chemistry, and surface charge. This was done by designing studies to assess toxic effects on intestinal epithelial cells after both acute and chronic exposure. The inorganic nanoparticles SiO2, TiO2, and ZnO were investigated. Particles were treated with digestive enzymes to simulate in vivo digestion before exposing to cells. Acute studies in which intestinal epithelial cells were treated with nanoparticles for 24 hours revealed no toxicity as measured by several different assays. Chronic studies in which cells were repeatedly treated with nanoparticles after each successive cell passage also revealed no toxicity or change in cell proliferation up to 32 nanoparticle exposures. These studies suggest that the investigated nanoparticles are not
Hyaluronic acid-coated bovine serum albumin nanoparticles loaded with brucine as selective nanovectors for intra-articular injection Zhipeng Chen,* Juan Chen,* Li Wu, Weidong Li, Jun Chen, Haibo Cheng, Jinhuo Pan, Baochang CaiDepartment of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China*These authors contributed equally to this workObjective: To evaluate the potential of hyaluronic acid (HA)-coated bovine serum albumin nanoparticles (BSANPs) as a novel chondrocyte-targeting drug-delivery nanomedicine.Methods: The HA-BSANPs were characterized by dynamic light scattering, transmission electron microscopy, differential scanning calorimetry, and X-ray diffraction. Fluorescence imaging was used to visualize the distribution of nanoparticles after intra-articular injection. The chondrocyte-targeting efficiency and cellular uptake mechanism of HA-BSANPs were investigated using endocytic inhibitors.Results: HA-BSANPs were successfully prepared with HA coating the
0007]Recently, the utilization of biological entities has emerged as a novel method for the synthesis of nanoparticles. Biotechnology approaches toward the synthesis of nanoparticles can have many advantages, such as a greater ease with which the process can be scaled up, economic viability, possibility of readily covering large surface areas by suitable growth of the mycelia, and its green chemistry nature. Some examples of the use of microbes and other biological entities in the synthesis of nanoparticles of different chemical compositions include the following: [0008]i. Ribosomes for biosynthesis of gold nanoparticles [I. S. Pavel, "Assembly of Gold Nanoparticles by Ribosomal Molecular Machines" Ph.D. Dissertation, The University of Texas at Austin, (May 2005)]; [0009]ii. Bacteria for production of cadmium sulfide, zinc sulfide, magnetite, iron sulfide and silver nanoparticles [D. Mandal, et al. "The use of microorganisms for the formation of metal nanoparticles and their ...
Nanoparticles (NPs) are finding their application worldwide for specific kind of scientific research irrespective of their Fields such as chemical industries, biomedical, engineering, Material science, Environmental science, health care, cosmetics, food and feed, drug-gene delivery, environment and health. Nanoparticles are in a way into each and every aspect of our life mainly due to their small size which gives them large surface area to volume ratio and makes them distinct from those of bulk materials. Among several noble metal nanoparticles, silver and gold nanoparticles have attained a special focus along with Biodegradable and Magnetic nanoparticles. The Metallic nanoparticles are synthesized using chemical and Mechanical methods which are highly toxic and costly compared to biological system, hence there is a need to have a set of standard procedures that can be used to produce NPs in an environmentally safe and cost effective way. Thus green synthesis of NPs using biological systems like ...
Magnetic nanoparticles are a class of nanoparticle that can be manipulated using magnetic fields. Such particles commonly consist of two components, a magnetic material, often iron, nickel and cobalt, and a chemical component that has functionality. While nanoparticles are smaller than 1 micrometer in diameter (typically 5-500 nanometers), the larger microbeads are 0.5-500 micrometer in diameter. Magnetic nanoparticle clusters that are composed of a number of individual magnetic nanoparticles are known as magnetic nanobeads with a diameter of 50-200 nanometers. Magnetic nanoparticle clusters are a basis for their further magnetic assembly into magnetic nanochains. The magnetic nanoparticles have been the focus of much research recently because they possess attractive properties which could see potential use in catalysis including nanomaterial-based catalysts, biomedicine and tissue specific targeting, magnetically tunable colloidal photonic crystals, microfluidics, magnetic resonance imaging, ...
Once these drug-toting nanoparticles have accumulated within a tumor, application of a magnetic field could trigger them to break down and release their anti-cancer arsenal.. "Its like a little Russian doll, with everything nested in there," Berti says.. Once these drug-toting nanoparticles have accumulated within a tumor, application of a magnetic field could trigger them to break down and release their therapeutic attack.. Theres even more to Bertis battle strategy, though. The temperature of iron oxide nanoparticles increases when theyre exposed to a magnetic field - one thats otherwise harmless to the body. Prompting the nanoparticles to heat up once theyve invaded a tumor could provoke further weakening of the cancer cells, thereby making malignancies even more susceptible to the chemotherapy drug.. Because the iron oxide nanoparticles are magnetic, their distribution could be monitored using magnetic resonance imaging (MRI), allowing clinicians to see the particles accumulated in a ...
Glucan particles (GPs) are hollow, porous 2-4 mum microspheres derived from the cell walls of Bakers yeast (Saccharomyces cerevisiae). The 1,3-beta-glucan outer shell provides for receptor-mediated uptake by phagocytic cells expressing beta-glucan receptors. GPs have been used for macrophage-targeted delivery of soluble payloads (DNA, siRNA, protein, and small molecules) encapsulated inside the hollow GPs via core polyplex and layer-by-layer (LbL) synthetic strategies. In this communication, we report the incorporation of nanoparticles as cores inside GPs (GP-NP) or electrostatically bound to the surface of chemically derivatized GPs (NP-GP). GP nanoparticle formulations benefit from the drug encapsulation properties of NPs and the macrophage-targeting properties of GPs. GP nanoparticle formulations were synthesized using fluorescent anionic polystyrene nanoparticles allowing visualization and quantitation of NP binding and encapsulation. Mesoporous silica nanoparticles (MSNs) containing the
Nanoparticle technology is being incorporated into many areas of molecular science and biomedicine. Because nanoparticles are small enough to enter almost all areas of the body, including the circulatory system and cells, they have been and continue to be exploited for basic biomedical research as well as clinical diagnostic and therapeutic applications. For example, nanoparticles hold great promise for enabling gene therapy to reach its full potential by facilitating targeted delivery of DNA into tissues and cells. Substantial progress has been made in binding DNA to nanoparticles and controlling the behavior of these complexes. In this article, we review research on binding DNAs to nanoparticles as well as our latest study on non-viral gene delivery using polyethylenimine-coated magnetic nanoparticles.
Nanodelivery of drugs, diagnostic agents and other functionalized nanoparticles aiming for better treatment of diseases or diagnosis purposes are the new trends in medicine that appear promising. However, nanoparticles or nanotechnology used to deliver these agents in vivo may have potential risks for cell and tissue damages. Thus, before nanotechnology is widely accepted as a routine therapeutic tool for effective medical treatment or for diagnostic tools this is mandatory to study their potential or plausible neurotoxic effects in details. So far effects of nanoparticles or nanomaterials including biodegradable nanoparticles on toxicity in the central nervous system (CNS) is not very well documented in the literature. Also, there is an urgent need to find dose related studies on nanoparticles on cellular toxicity especially in vivo situations. Without these details and systematic studies, the use of nanomedicine still remains questionable. There are also reports that drugs delivered through different
TY - CHAP. T1 - Nanoparticles for mass spectrometry applications. AU - Larguinho, Miguel. AU - Capelo, José Luís. AU - Baptista, Pedro V.. N1 - Sem PDF.. PY - 2015/9/18. Y1 - 2015/9/18. N2 - Nanotechnology has led to the development of new and improved materials, and particular emphasis has been directed toward nanoparticles and their multiple bio-applications. Nanoparticles exhibit size-, shape-, and compositiondependent properties, e.g., surface plasmon resonance and photothermal properties, which may potentially enhance laser desorption/ionization systems for mass spectrometry-based analysis of biomolecules. Also, nanoparticles possess high surface to volume ratio that can be easily derivatized with a wide range of ligands with different functional groups. Surface modification makes nanoparticles advantageous for sample preparation procedures prior to detection by mass spectrometry. Moreover, it allows the synthesis of affinity probes, which promotes interactions between nanoparticles and ...
Cisplatin is the most widely used anticancer drug, but its side effects limit the maximum systemic dose. To circumvent the side effects, a DNA tetrahedron-affibody nanoparticle was prepared by combination of a DNA chain with cisplatin via interstrand crosslinks or adducts. Each nanocarrier can bind ∼68 molecules of
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Creative Diagnostics, a global leading manufacturer of highly uniform nanoparticles widely used in biology and medicine, now launches a new product line of coating nanoparticles to support researchers in the areas of targeted drug delivery, magnetic resonance imaging (MRI), cell labelling and separation, and hyperthermia treatment of cancer, etc.. Magnetic nanoparticles are superparamagnetic in nature; they are magnetized only upon application of an external magnetic field. Due to large surface area, magnetic nanoparticles are prone to oxidation by air and aggregation; this limits their use in biomedical applications. Various coating strategies have been employed to protect magnetic nanoparticles from oxidation and aggregation by using polymers and noble metals like gold, silica.. Out of them, gold coating offers advantage of being chemically stable and biocompatible. It also provides the possibility of conjugating targeting ligands, drugs using gold-thiol chemistry, and for photothermal ...