Accepted name: DNA-3-methyladenine glycosylase II. Reaction: Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine. Other name(s): deoxyribonucleate 3-methyladenine glycosidase II; 3-methyladenine DNA glycosylase II; DNA-3-methyladenine glycosidase II; AlkA. Systematic name: alkylated-DNA glycohydrolase (releasing methyladenine and methylguanine). Comments: Involved in the removal of alkylated bases from DNA in Escherichia coli (cf. EC 2.1.1.63 methylated-DNA [protein]-cysteine S-methyltransferase).. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 89287-38-7. References:. 1. Evensen, G. and Seeberg, E. Adaptation to alkylation resistance involves the induction of a DNA glycosylase. Nature 296 (1982) 773-775. [PMID: 7040984]. 2. Karran, P., Hjelmgren, T. and Lindahl, T. Induction of a DNA glycosylase for N-methylated purines is part of the adaptive response to alkylating agents. Nature 296 (1982) 770-773. ...
Endonuclease III; DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N- glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3 to the AP site by a beta-elimination, leaving a 3-terminal unsaturated sugar and a product with a terminal 5-phosphate (219 aa ...
Involved in the removal of alkylated bases from DNA in Escherichia coli (cf. EC 2.1.1.63 methylated-DNA-[protein]-cysteine S-methyltransferase).
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
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Excellgen E. coli Uracil DNA Glycosylase, UDG [EG-1019] - Description E. coli Uracil-DNA Glycosylase (UDG), also known as Uracil N-Glycosylase (UNG), catalyses the release of uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from short oligonucleotides (|6 bases), or RNA substrates. Applications Digestion of uracil-containing DNA Source An E. coli strain that carries the ung (Uracil-DNA Glycosylase)
Uracil, a promutagenic base, appears in DNA either by deamination of cytosine or by incorporation nof dUMP by DNA polymerases. This unconventional base in DNA is removed by uracil-DNA glycosylase (UDG). Interestingly, a bacteriophage-encoded short polypeptide, UDG inhibitor (Ugi), specifically inhibits UDGs by forming a tight complex. Three-dimensional structures of the complexes of Ugi with UDGs from Escherichia coli, human and herpes simplex virus have shown that two of the structural elements in Ugi, the hydrophobic pocket and the b1-edge, establish key interactions with UDGs. In this report the characterization of complexes of Ugi with UDGs from Mycobacterium tuberculosis, a pathogenic bacterium, and Mycobacterium smegmatis, a widely used model organism for the former, is described. Unlike the E. coli (Eco) UDG-Ugi complex, which is stable to treatment with 8 M urea, the mycobacterial UDG-Ugi complexes dissociate in 5-6 M urea. Furthermore, the Ugi from the complexes of mycobacterial UDGs ...
The pyrimidine salvage enzyme, nucleoside hydrolase, is catalyzes the irreversible hydrolysis of nucleosides into the free nucleic acid base and D-ribose. Nucleoside hydrolases have varying degrees of specificity towards purine and pyrimidine nucleosides. In E. coli, three genes were found that encode homologues of several known nucleoside hydrolases in protozoa. All three genes (designated yaaF, yeiK, and ybeK) were amplified by PCR and cloned. Two of the gene products (yeiK and ybeK) encode pyrimidine-specific nucleoside hydrolases, while the third (yaaF) encodes a nonspecific nucleoside hydrolase. All three were expressed at low levels and had different modes of regulation. As a comparative analysis, the homologous genes of Pseudomonas aeruginosa and P. fluorescens (designated nuh) were cloned. Both were determined to encode nonspecific nucleoside hydrolases. The nucleoside hydrolases of the pseudomonads exhibited markedly different modes of regulation. Both have unique promoter structures and
K00567 ogt; methylated-DNA-[protein]-cysteine S-methyltransferase [EC:2.1.1.63] K03648 UNG; uracil-DNA glycosylase [EC:3.2.2.27] K03575 mutY; A/G-specific adenine glycosylase [EC:3.2.2.31] K10773 NTH; endonuclease III [EC:4.2.99.18] K10844 ERCC2; DNA excision repair protein ERCC-2 [EC:3.6.4.12] K03574 mutT; 8-oxo-dGTP diphosphatase [EC:3.6.1.55] K03574 mutT; 8-oxo-dGTP diphosphatase [EC:3.6.1.55] K03574 mutT; 8-oxo-dGTP diphosphatase [EC:3.6.1.55] K03574 mutT; 8-oxo-dGTP diphosphatase [EC:3.6.1.55] K03574 mutT; 8-oxo-dGTP diphosphatase [EC:3.6.1.55] K03574 mutT; 8-oxo-dGTP diphosphatase [EC:3.6.1.55] K03574 mutT; 8-oxo-dGTP diphosphatase [EC:3.6.1.55] K03574 mutT; 8-oxo-dGTP diphosphatase [EC:3.6.1.55] K01520 dut; dUTP pyrophosphatase [EC:3.6.1.23] K03649 mug; double-stranded uracil-DNA glycosylase [EC:3.2.2.28] K01247 alkA; DNA-3-methyladenine glycosylase II [EC:3.2.2.21] K10563 mutM; formamidopyrimidine-DNA glycosylase [EC:3.2.2.23 4.2.99.18] K10563 mutM; formamidopyrimidine-DNA glycosylase ...
Human DNA topoisomerase II-binding protein 1 (hTopBP1) plays an important role in DNA replication and the DNA damage checkpoint pathway. The human mutY homolog (hMYH) is a base excision repair DNA glycosylase that excises adenines or 2-hydroxyadenines that are mispaired with guanine or 7,8-dihydro-8-oxoguanine (8-oxoG). hTopBP1 and hMYH were involved in ATR-mediated Chk1 activation, moreover, both of them were associated with ATR and hRad9 which known as checkpoint-involved proteins. Therefore, we investigated whether hTopBP1 interacted with hMYH, and what the function of their interaction is. We documented the interaction between hTopBP1 and hMYH and showed that this interaction increased in a hydroxyurea-dependent manner. We also mapped the hMYH-interacting region of hTopBP1 (residues 444-991). In addition, we investigated several cell cycle-related proteins and found that co-knockdown of hTopBP1 and hMYH significantly diminished cell cycle arrest due to compromised checkpoint kinase 1 (Chk1)
1LQG: Domain closure and action of uracil DNA glycosylase (UDG): structures of new crystal forms containing the Escherichia coli enzyme and a comparative study of the known structures involving UDG.
1LQG: Domain closure and action of uracil DNA glycosylase (UDG): structures of new crystal forms containing the Escherichia coli enzyme and a comparative study of the known structures involving UDG.
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Nickel atom in PDB 1kic: Inosine-Adenosine-Guanosine Preferring Nucleoside Hydrolase From Trypanosoma Vivax: ASP10ALA Mutant in Complex With Inosine
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3- and 5-phosphates.
DNA glycosylases are involved in the base excision repair pathway, and all mammals express multiple DNA glycosylases to maintain genome stability. However, the simultaneous detection of multiple DNA glycosylase still remains a great challenge. Here, we develop a single-molecule detection method for the simul
MPG antibody (N-methylpurine-DNA glycosylase) for ICC/IF, WB. Anti-MPG pAb (GTX101916) is tested in Human samples. 100% Ab-Assurance.
To demonstrate the simultaneous detection of multiple DNA glycosylases, we used hOGG1 and hAAG as model enzymes. hOGG1 and hAAG may initiate the first step of base excision repair, and are considered to be functional biomarkers for lung cancer.12-14 The principle of the DNA glycosylase assay is illustrated in Scheme 1. This assay involves two steps: (1) the DNA glycosylase-mediated cleavage of molecular beacons and (2) the subsequent single-molecule detection. We designed two specific substrates for hOGG1 and hAAG, respectively. The substrate for hOGG1 is labeled with a Cy3 fluorophore at the 5′ terminus and a BHQ2 quencher at the 3′ terminus, and is modified with 8-oxoG positioned 6 deoxynucleotides downstream of a 5′-terminus. The substrate for hAAG is labeled with a Cy5 fluorophore at the 5′ terminus and a BHQ3 quencher at the 3′ terminus, and is modified with deoxyinosine positioned 5 deoxynucleotides downstream of a 5′-terminus (Scheme 1). The substrate for hOGG1 and the ...
References for Abcams Recombinant |em|E. coli |/em| mutM protein (ab124310). Please let us know if you have used this product in your publication
Versées, W., J. Barlow, and J. Steyaert, Transition-state complex of the purine-specific nucleoside hydrolase of T. vivax: enzyme conformational changes and implications for catalysis., J Mol Biol, vol. 359, issue 2, pp. 331-46, 2006 Jun 2. ...
DNA glycosylases help maintain the genome by excising chemically modified bases from DNA. In bacteria, DNA glycosylases that remove alkylated nucleobases have varying substrate specificities despite their structural similarity. Escherichia coli 3
Synthetic sickness/lethality (SSL) can be exploited to develop therapeutic strategies for cancer. Deficiencies in the tumor suppressor proteins MLH1 and MSH2 have been implicated in cancer. Here we demonstrate that deficiency in MSH2 is SSL with inhibition of the DNA polymerase POLB, whereas deficiency in MLH1 is SSL with DNA polymerase POLG inhibition. Both SSLs led to the accumulation of 8-oxoG oxidative DNA lesions. MSH2/POLB SSL caused nuclear 8-oxoG accumulation, whereas MLH1/POLG SSL led to a rise in mitochondrial 8-oxoG levels. Both SSLs were rescued by silencing the adenine glycosylase MUTYH, suggesting that lethality could be caused by the formation of lethal DNA breaks upon 8-oxoG accumulation. These data suggest targeted, mechanism-based therapeutic approaches.. ...
Versées, W., K. Decanniere, R. Pellé, J. Depoorter, E. Brosens, D. W. Parkin, and J. Steyaert, Structure and function of a novel purine specific nucleoside hydrolase from Trypanosoma vivax., J Mol Biol, vol. 307, issue 5, pp. 1363-79, 2001 Apr 13. ...
Noncysteinyl coordination to the [4Fe-4S]2+ cluster of the DNA repair adenine glycosylase MutY introduced via site-directed mutagenesis. Structural characterization of an unusual histidinyl-coordinated cluster ...
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Viruses employ an array of elaborate strategies to overcome plant defense mechanisms and must adapt to the requirements of the host translational systems. Pokeweed antiviral protein (PAP) from Phytolacca americana is a ribosome inactivating protein (RIP) and is an RNA N-glycosidase that removes specific purine residues from the sarcin/ricin (S/R) loop of large rRNA, arresting protein synthesis at the translocation step. PAP is thought to play an important role in the plants defense mechanism against foreign pathogens. This review focuses on the structure, function, and the relationship of PAP to other RIPs, discusses molecular aspects of PAP antiviral activity, the novel inhibition of this plant toxin by a virus counteraction-a peptide linked to the viral genome (VPg), and possible applications of RIP-conjugated immunotoxins in cancer therapeutics.
Two enzymes in the methionine salvage pathway, 5-methylthioribose kinase (MTR kinase) and 5´-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTA/SAH nucleosidase) were purified from Klebsiellapneumoniae. Chromatography using a novel 5´-(p-aminophenyl)thioadenosine/5-(p-aminophenyl)thioribose affinity matrix allowed the binding and selective elution of each of the enzymes in pure form. The molecular mass, substrate kinetics and N-terminal amino acid sequences were characterized for each of the enzymes. Purified MTR kinase exhibits an apparent molecular mass of 46-50 kDa by SDS/PAGE and S200HR chromatography, and has a Km for MTR of 12.2 μM. Homogeneous MTA/SAH nucleosidase displays a molecular mass of 26.5 kDa by SDS/PAGE, and a Km for MTA of 8.7 μM. Comparisons of the N-terminal sequences obtained for each of the enzymes with protein-sequence databases failed to reveal any significant sequence similarities to known proteins. However, the amino acid sequence obtained for the ...
The bacterial 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) enzyme is a multifunctional enzyme that catalyzes the hydrolysis of the N-ribosidic bond of at least four different adenosine-based metabolites: S-adenosylhomocysteine (S
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Macromolecule FORMYCIN-5-MONOPHOSPHATE C 1 0 H 1 4 N 5 O 7 P PBAHXXBYQACZMA-KSYZLYKTSA-N The hypoxanthine phosphoribosyltransferase (HPRT) from Trypanosoma cruzi, etiologic agent of Chagas disease, was cocrystallized with the inosine analogue Formycin B 1NC3: Structure of Escherichia coli 5-methylthioadenosine/ S-adenosylhomocysteine nucleosidase inhibitor complexes provide insight into the conformational changes Previous article in issue: 2-Methylene-3-quinuclidinone and its derivatives Previous article in issue: 2-Methylene-3-quinuclidinone and its derivatives Next article Abstract. Biochemistry. , Townsend, L. K. About DrugBank; Wishart Research Group; Structure, properties, spectra, suppliers and links for: formycin A, 6742-12-7. Formycin 5-triphosphate (FoTP), a fluorescent analog of ATP, is shown to be a substrate for the membrane-bound adenylate cyclase . Synonyms: Not Acetyldigitoxin may decrease the cardiotoxic activities of Formycin. Crystal structure of formycin 5′-phosphate: An ...
B cells that mediate normal, T cell-dependent, humoral immune responses must first pass through germinal centers (GCs) within the cortex of antigenically stimulated lymph nodes. As they move through the dark zone and then the light zone in the GC, B cells are subjected to somatic hypermutation and switch recombination within their rearranged immunoglobulin genes and also participate in a number of other processes that control development into memory cells or cells specialized for antibody secretion. To investigate the molecular mechanisms that contribute to B cell development within GCs, we constructed a recombinant DNA library enriched for cDNAs derived from human genes expressed in B cells at this site. This library was found to contain a cDNA structurally and functionally related to genes in bacteria and yeast for the DNA repair enzyme 8-oxoguanine DNA glycosylase. Northern blot analysis indicated that the human gene is expressed as two alternatively spliced messenger RNAs within GC B cells at levels
甲硫核苷酶為一種酵素,可以催化5-methylthioadenosine 和S-adenosylhomocysteine的水解脫腺苷酸化的反應。甲硫核苷酶也被視為抗細菌藥物發現的目標,因為甲硫核苷酶對於細菌代謝系統是非常重要的,且也只有細菌會產生。[7]視腺嘌呤的氮和核醣的碳距離不同,過渡態的結構可藉由早期或晚期的解離程度決定。根據找到不同的過渡態結構,Schramm和他的同事設計了兩個過渡態類似物,模仿早期即晚期的解離過渡態。早期即晚期的解離過渡態展現了個別的解離常數,約在360~140pM。[8]. ...
Since the discovery in 1974 of uracil DNA glycosylase (UDG), the first member of the family of enzymes involved in base excision repair (BER), considerable progress has been made in the understanding of DNA glycosylases, the polypeptides that remove damaged or mispaired DNA bases from DNA. We also know the enzymes that act downstream of the glycosylases, in the processing of abasic sites, in gap filling and in DNA ligation. This article covers the most recent developments in our understanding of BER, with particular emphasis on the mechanistic aspects of this process, which have been made possible by the elucidation of the crystal structures of several glycosylases in complex with their respective substrates, substrate analogues and products. The biological importance of individual BER pathways is also being appreciated through the inactivation of key BER genes in knockout mouse models. ...
8-oxoguanine DNA glycosylase (OGG1) is found in many species and catalyzes the excision of 8-oxoguanine from DNA. This modified base byproduct occurs as a result of exposure to reactive oxygen species. Alternative splicing of the C-terminal region of the OGG1 gene in humans results in two major groups of variants: type 1 and type 2. Both groups share a common N-terminal region that contains a mitochondrial targeting sequence required for OGG1 localization in mitochondria. Although knockout mice lacking the OGG1 gene were observed to have a normal lifespan, the precise role of OGG1 in mutagenesis and cancer remains controversial. OGG1 is also known as 8-hydroxyguanine DNA glycosylase, DNA-apurinic or apyrimidinic site lyase, AP lyase, N-glycosylase/DNA lyase, HMMH, MMH, MUTM, OGH1, and HOGG1.. ...
8-oxoguanine DNA glycosylase (OGG1) is found in many species and catalyzes the excision of 8-oxoguanine from DNA. This modified base byproduct occurs as a result of exposure to reactive oxygen species. Alternative splicing of the C-terminal region of the OGG1 gene in humans results in two major groups of variants: type 1 and type 2. Both groups share a common N-terminal region that contains a mitochondrial targeting sequence required for OGG1 localization in mitochondria. Although knockout mice lacking the OGG1 gene were observed to have a normal lifespan, the precise role of OGG1 in mutagenesis and cancer remains controversial. OGG1 is also known as 8-hydroxyguanine DNA glycosylase, DNA-apurinic or apyrimidinic site lyase, AP lyase, N-glycosylase/DNA lyase, HMMH, MMH, MUTM, OGH1, and HOGG1.. ...
Formamidopyrimidine-DNA glycosylase (Fapy-DNA glycosylase)(gene fpg) is a bacterial enzyme involved in DNA repair and which excise oxidized purine bases to release 2,6-diamino-4-hydroxy-5N-methylformamido-pyrimidine (Fapy) and 7,8-dihydro-8-oxoguanine (8-OxoG) residues. In addition to its glycosylase activity, FPG can also nick DNA at apurinic/apyrimidinic sites (AP sites). FPG is a monomeric protein of about 32 Kd which binds and require zinc for its activity ...
Cytotoxic biotherapeutic agents effective for treating certain types of cancer in humans are provided which comprise the TP-3 murine monoclonal antibody chemically conjugated to pokeweed antiviral protein (PAP). The invention further provides a method which utilizes the disclosed cytotoxic biotherapeutic agents to systemically treat cancer patients. With slight modifications the method of the present invention should be generally applicable to preparation and use of other cytotoxic biotherapeutic agents using chemical or recombinant derivatives of the TP-3 or TP-1 antibodies or PAP toxin. The invention is applicable to cancer patients who express the p80 antigen recognized by the TP-1/TP-3 antibodies either on the surface of their tumor cells or on the tumor blood vessels.
The pyrimidine-specific nucleoside hydrolase Yeik (CU-NH) from Escherichia coli cleaves the N-glycosidic bond of uridine and cytidine with a 102~104-fold faster than that of purine nucleoside substrates such as inosine. Such remarkable substrate specificity and the plausible hydrolytic mechanisms of uridine have been explored by using QM/MM and MM MD simulations. The present calculations show that the relatively stronger hydrogen bond interactions between uridine and the active-site residues Gln227 and Tyr231 in CU-NH play an important role in enhancing the substrate binding and thus promoting the N-glycosidic bond cleavage, in comparison with inosine ...
Homo sapiens 8-oxoguanine DNA glycosylase (OGG1), nuclear gene encoding mitochondrial protein, transcript variant 2e, mRNA. (H00004968-R08) - Products - Abnova
NucleoMix (dUTP), PCR Grade, is designed for amplification reactions where high-quality reagents are required, such as for in vitro diagnostics. The ready-to-use mix eliminates process steps. Avoid carryover contamination between PCRs, a significant source of false positives. To decontaminate PCR and RT-PCR reagent mixes, dUTP is incorporated in place of dTTP. Subsequent reactions should be treated with Uracil-DNA Glycosylase (UNG) before amplification to degrade dUTP-containing PCR products ...
Complete information for UNGP1 gene (Pseudogene), Uracil-DNA Glycosylase Pseudogene 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Mouse anti Human Cdk6 antibody, clone DCS-83 recognizes the human cyclin dependent kinase 6, also known as Cdk6 or Serine/threonine-protei
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Day 6 contains 99 protein-coding genes, including MUTYH (mutY homolog). Throughout life, your cells suffer DNA damage, which is constantly repaired by enzymes - one of which is made by the gene MUTYH.. When the DNA encoding a repairer like MUTYH is itself mutated, though, mutations can start to run amok in the genome, leading to cancer. Inherited MUTYH variants are associated with polyposis and colon cancer.. MUTYH is named after the mutY gene in E. coli bacteria. The similarity to a bacterial gene means that it is as ancient as the common ancestor of prokaryotes and eukaryotes (,1.7 billion years.). Click here to see your MUTYH gene where you will see a cancer-associated variant flashing.. ...
Uses, Benefits, Cures, Side Effects, Nutrients in Pokeweed. List of various diseases cured by Pokeweed. How Pokeweed is effective for various diseases is listed in repertory format. Names of Pokeweed in various languages of the world are also given.
HRP偶联Saporin抗体(ab42903)经WB, IHC实验严格验证,被4篇文献引用,实验条件参看说明书。Abcam对所有产品均提供质保服务和专属技术支持,中国75%以上现货。
Learn more about Pokeroot at St. Marks Hospital Pokeweed Uses Principal Proposed Uses We recommend against using pokeroot at all. ...
Learn more about Pokeroot at Memorial Health Pokeweed Uses Principal Proposed Uses We recommend against using pokeroot at all. ...
Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA-3-methyladenine glycosylase (TIGR00567; EC 3.2.2.-; HMM-score: 156.9) ...