Abstract:Background: The metabolic syndrome comprises an array of cardiovascular disease (CVD) risk; the clustering of CVD risk factors in the metabolic syndrome suggests a common underlying mechanism. Circulating serum sialic acid, an inflammatory marker has recently been shown to be a strong predictor of cardiovascular mortality, and obesity-related diseases. Objective: is to study the relationship between serum total sialic acid and metabolic variables in type 2 diabetes with and without metabolic syndrome.Subject and Methods: One hundred type 2 diabetic mellitus patients (52 of them had metabolic syndrome and 48 without it) were recruited from the National Diabetic Center, Al-Mustansiriya University during the period from June 2011 to January 2012. One hundred apparently healthy control matched for age and sex were participated in this study. Fasting venous blood samples were collected from all participants, the level of HbA1c was estimated by high performance liquid chromatography. Total ...
Lindberg and colleagues report on a fascinating observation that serum sialic acid level predicts cardiovascular (and noncardiovascular) mortality. The studys classical design of measuring a factor and relating it to subsequent mortality was greatly strengthened by its large community-based sample. However, this study raises several concerns. First, it is unclear whether any hypothesis about sialic acid had been set out by the investigators 20 years ago or whether this was part of the series of protein measurements that was done without a particular hypothesis. Second, because the measurement of sialic acid was done in the 1960s, another sialated protein may have been measured. Third, many more risk factors for coronary disease are known now than were known 20 years ago; for example, the only lipid measured in that study was total cholesterol. Thus, the relation of other lipids and lipoproteins to sialic acid could not be determined. Also, no data on cigarette smoking were provided. Fourth, ...
We have generated two transgenic mice strains (one is overexpressing the mutated key enzyme of the sialic acid biosynthesis, which leads to high sialic acid levels and one has a defect in the sialic acid biosynthesis). Both strains will be compared with wild-type animals. We plan to analyse O-GlcNAc, sialic acid, sialic acid binding proteins and sialic acid-dependent differentiation markers in all organs over the whole lifespan and quantify age-dependent muscle performance in vivo. The outcome of metabolic sialic acid engineering will be analysed in embryonic stem cells (differentiation) and neuronal cells (neurite outgrowth e.g. regeneration). We also plan to analyse the involvement of sialylation and O-GlcNAcylation on the function of endothelial cells. After metabolic engineering we will analyse their barrier capacity and age-related impact on neuronal cells by real-time cell analysis. Since high levels of glucose induce glycation of proteins, we will analyse the function of an artificial ...
We have generated two transgenic mice strains (one is overexpressing the mutated key enzyme of the sialic acid biosynthesis, which leads to high sialic acid levels and one has a defect in the sialic acid biosynthesis). Both strains will be compared with wild-type animals. We plan to analyse O-GlcNAc, sialic acid, sialic acid binding proteins and sialic acid-dependent differentiation markers in all organs over the whole lifespan and quantify age-dependent muscle performance in vivo. The outcome of metabolic sialic acid engineering will be analysed in embryonic stem cells (differentiation) and neuronal cells (neurite outgrowth e.g. regeneration). We also plan to analyse the involvement of sialylation and O-GlcNAcylation on the function of endothelial cells. After metabolic engineering we will analyse their barrier capacity and age-related impact on neuronal cells by real-time cell analysis. Since high levels of glucose induce glycation of proteins, we will analyse the function of an artificial ...
Variations of serum sialic acid level in liver cirrhosis. Saatea Arif, Najeeb-ul-Haq, Ruhila Hanif, A. Sattar Khan, Jamil-ur-Rehman, Taufeeq Ahmed Mufti. Comparison of students performance under composite scheme and split scheme of first professional MBBS. examination. Tehseen Iqbal, Naila Hamid, Hamid Javaid ...
Rapid shifts in microbial composition frequently occur during intestinal inflammation, but the mechanisms underlying such changes remain elusive. Here we demonstrate that an increased caecal sialidase activity is critical in conferring a growth advantage for some bacteria including Escherichia coli (E. coli) during intestinal inflammation in mice. This sialidase activity originates among others from Bacteroides vulgatus, whose intestinal levels expand after dextran sulphate sodium administration. Increased sialidase activity mediates the release of sialic acid from intestinal tissue, which promotes the outgrowth of E. coli during inflammation. The outburst of E. coli likely exacerbates the inflammatory response by stimulating the production of pro-inflammatory cytokines by intestinal dendritic cells. Oral administration of a sialidase inhibitor and low levels of intestinal α2,3-linked sialic acid decrease E. coli outgrowth and the severity of colitis in mice. Regulation of sialic acid ...
HA-mediated attachment of influenza viruses to sialic acid-containing receptors on the host cell surface is the initial step in infection. Influenza virus HA contains at the tip a narrow crevice lined with highly conserved amino acids. By its ability to specifically bind sialic acids, this crevice has been identified as the receptor-binding site (7, 31, 37). The precise structure of this HA domain is known to be of crucial importance for the process of virus binding to its receptor. Accordingly, single-amino-acid substitutions in the binding pocket can result in altered receptor-binding specificity and altered host range of the viruses (1, 5, 35). Furthermore, in our previous study employing vector-expressed FPV HA, we could show that oligosaccharides flanking the binding site modulate receptor affinity (26). The aim of the present study was to evaluate the impact of each individual N-glycan at the FPV HA tip on the growth of intact viruses. To address this question, we generated recombinant ...
Increased risk for chronic comorbid disorders in patients with inflammatory arthritis: a population based study. Ursum, Jennie; Nielen, Mark M. J.; Twisk, Jos W. R.; Peters, Mike J. L.; Schellevis, François G.; Nurmohamed, Michael T.; Korevaar, Joke C. // BMC Family Practice;2013, Vol. 14 Issue 1, p199 Background: Studies determining the development of a wide variety of different comorbid disorders in inflammatory arthritis (IA) patients are scarce, however, this knowledge could be helpful in optimising preventive care in IA patients. The aim of this study is to establish the risk that new... ...
Sialoglycoproteins (SGs) have been shown to be important in the enhancement of sensitivity to sialic acid-specific toxin from E. histolytica (Feingold et al. 1984). This toxin is known to induce fluid secretion in ligated intestinal loops of indomethacin-pretreated rats. Since SGs serve as receptors for the E. histolytica toxin, the pathogenesis of amebiasis could be preceded by an initial unmasking event of surface sialic acid by the parasites sialidase, followed by attachment of the toxin. Feingold et al. (1985) reported the isolation of two cytotoxic factors from E. histolytica trophozoites, one of which is sensitive to sialic acid levels above 5 mg/ml. Our observation could imply that, when such a threshold level of free liberated by sialidase is attained, it triggers the expression of PSL to hydrolyse the sialic acid to pyruvate and N-acetyl mannosamine. This biochemical event may promote the interaction of cytopathic factors with the receptor. Moreover, when Neu5Ac2,3-lac was incubated ...
Sialoglycoproteins (SGs) have been shown to be important in the enhancement of sensitivity to sialic acid-specific toxin from E. histolytica (Feingold et al. 1984). This toxin is known to induce fluid secretion in ligated intestinal loops of indomethacin-pretreated rats. Since SGs serve as receptors for the E. histolytica toxin, the pathogenesis of amebiasis could be preceded by an initial unmasking event of surface sialic acid by the parasites sialidase, followed by attachment of the toxin. Feingold et al. (1985) reported the isolation of two cytotoxic factors from E. histolytica trophozoites, one of which is sensitive to sialic acid levels above 5 mg/ml. Our observation could imply that, when such a threshold level of free liberated by sialidase is attained, it triggers the expression of PSL to hydrolyse the sialic acid to pyruvate and N-acetyl mannosamine. This biochemical event may promote the interaction of cytopathic factors with the receptor. Moreover, when Neu5Ac2,3-lac was incubated ...
Methods and Materials This review targets the role of antibody methods and sialylation because of its quantitation. this lectin-affinity small fraction holds the entire anti-inflammatory activity, with the nonbinding fraction being essentially ineffective. At first glance, these results appear to match nicely with the 11% of sialylated N-glycans found in the Fc region [8, 22]. The situation, however, is more complex. Site-specific analysis of the SNA binding and nonbinding fractions of IVIG revealed no significant difference in Fc sialylation [22]. The obvious conclusion was that the fractionation of IgG on SNA was solely based on the N-glycans in the variable domains, whereas the sialoglycans in the CH2 domain name were inaccessible to the lectin. This view was seemingly supported by SNA fractionation of isolated Fab fragments [22]. However, it harshly contradicts the earlier conclusion that this anti-inflammatory activity depended on sialylation of the Fc region N-glycans [3, 4]. Stadlmann and ...
Sigel et al.(J. Am. Chern. Soc., 118(16), 3789-3800,1996) reported the efficacy of polymers containing sialoside groups in inhibiting the adhesion of influenza virus to, erythrocytes. They delineated the contributions of enhanced substrate ligand binding and steric considerations to efficiency of inhibition. These investigators reported sialic acid ligands, which can be exploited for the inhibition of the influenza virus. Monomeric inhibitor requires a higher concentration for inhibition since they are required to occupy at least half of the sialic acid binding sites on the virus, whereas the high molecular weight inhibitors need only a few attachments to achieve the same. Dimick et al. (J. Am. Chern. Soc., 121: 44,10286,1999) reported the molecular cluster glycoside effects, and the synthesis of polyvalent ligands for the plant lectin concanavalin A ...
Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries. ...
In N-acetylneuraminic acid, apart from O9 and O8, a possible glycosylation site is the O4 position. For example, gangliosides HLG-2 and HPG-7 are considered to be potential lead compounds for carbohydrate-based drug development to treat neural disorders. However, the construction of their α(1 → 4) fucosyl sialic acid and α(2 → 4) linkages between sialic acids is difficult because of the regioselec ...
Sialic acids are prominent termini of mammalian glycoconjugates and are key binding determinants for cell-cell recog-nition lectins. Binding of the sialic acid-dependent lectin, myelin-associated glycoprotein (MAG), to nerve cells is implicated in the inhibition of nerve regeneration after injury. Therefore, blocking MAG binding to nerve cell sialoglycoconjugates might enhance nerve regeneration. Previously, we reported that certain sialoglycoconjugates bearing N-acetylneuraminic acid (NeuAc) but not N-glycolylneuraminic acid (NeuGc) support MAG binding (Collins et al., 1997a). We now report highly efficient conversion of sialic acids on living neural cells from exclusively NeuAc to predominantly NeuGc using a novel synthetic metabolic precursor, N-glycolylmannosamine pentaacetate (Man-NGc-PA). When NG108-15 neuroblastoma-glioma hybrid cells, which normally express only NeuAc (and bind to MAG), were cultured in the presence of 1 mM ManNGcPA, they expressed 80-90% of their sialic acid precursor pool as
Despite the functional importance of brain sialylation, in vivo visualization of sialoglycans in the mouse brain has been impossible. Azidosugars have been used as tracers to report the sialic acid biosynthesis in living animals (12, 13), but the BBB impedes their access to the brain. The LABOR strategy developed herein overcomes this obstacle by exploiting liposomes to shuttle 9AzSia into the brain. Previous in vitro studies using neuron cell culture (33) and brain tissue culture (34) have shown that neurons can uptake azidosugars and metabolically incorporate them into sialoglycans. Our results demonstrate that LABOR-mediated delivery of 9AzSia into the brain results in robust metabolic incorporation of azides into brain sialoglycans in living mice. In vivo copper-free click chemistry enables whole-body fluorescence imaging of brain sialylation. This strategy may be further applied to visualize sialylation in mouse models of brain tumors and neurodegenerative diseases (8, 10).. The mouse ...
Most adenoviruses use the coxsackie-adenovirus receptor (CAR) as a major cellular receptor. We have shown recently that adenovirus types 8, 19a, and 37, which are the major causes of epidemic keratoconjunctivitis, use sialic acid rather than CAR as a major cellular receptor. The predicted isoelectric point of the receptor-interacting knob domain in the adenovirus fiber protein is unusually high (9.0-9.1) in type 8, 19a, and 37. The pKa of sialic acid is low, 2.6, implying a possible involvement of charge in fiber knob-sialic acid interactions. Here we show that (i) positively charged adenovirus knobs require sialic acid for efficient cell membrane interactions; (ii) viral and knob interactions with immobilized sialic acid or cell-surface sialic acid are sensitive to increased ionic strength; (iii) negatively charged molecules such as sulfated glycosaminoglycans inhibit the binding of virions to target cells in a nonspecific, charge-dependent manner; and that (iv) the ability of adenovirus knobs ...
Iwata, Y., Suzuki, O. and Wakabayashi, S. (2013), Decreased surface sialic acid content is a sensitive indicator of muscle damage. Muscle Nerve, 47: 372-378. doi: 10.1002/mus.23632 ...
Tumor cells of various origins feature increased expression of sialic acid sugars on membrane glycoproteins and glycolipids and their secretion into the tumor microenvironment. Sialic acids are synthesized in and expressed by essentially every vertebrate cell, and are involved in multiple different physiologic processes. However, hypersialylation of tumor cells relative to their untransformed normal counter parts specifically benefits tumor cell growth and correlates with a poor prognosis for patients with cancer (1, 2). Sialic acids comprise a family of more than 50 carbohydrates that share a nine-carbon backbone (C1-9) to which specific chemical modifications are enzymatically attached inside the cell. The most common sialic acid derivate found in mammals is N-Acetylneuraminic acid (Neu5Ac) which bears an acetyl group on the fifth carbon atom (C5). In general, sialic acids terminate the outer end of glycans (sialoglycans), where they are enzymatically linked to other carbohydrates, such as the ...
Chloramphenicol Role of serum total sialic acid in differentiating cholangiocarcinoma from hepatocellular carcinoma The remaining methylene and methine protons appeared as a multiplet at ? Its classified as a gastrointestinal disorder that is not widely recognized by mainstream medical doctors. ligaments and tendons slowly heal with continued consumption of sour cherry juice for arthritis. medicate and do not presume. chloromycetin order chloramphenicol online cod and strawberries are fresh fruits and excellent sources of fiber. chloramphenicol buy chloramphenicol cheap with no ...
Chloramphenicol Role of serum total sialic acid in differentiating cholangiocarcinoma from hepatocellular carcinoma The remaining methylene and methine protons appeared as a multiplet at ? Its classified as a gastrointestinal disorder that is not widely recognized by mainstream medical doctors. ligaments and tendons slowly heal with continued consumption of sour cherry juice for arthritis. medicate and do not presume. chloromycetin order chloramphenicol online cod and strawberries are fresh fruits and excellent sources of fiber. chloramphenicol buy chloramphenicol cheap with no ...
In this study, we investigated the use of metabolic oligosaccharide engineering and bio-orthogonal ligation reactions combined with lectin microarray and mass spectrometry to analyze sialoglycoproteins in the SW1990 human pancreatic cancer line. Specifically, cells were treated with the azido N-acetylmannosamine analog, 1,3,4-Bu3ManNAz, to label sialoglycoproteins with azide-modified sialic acids. The metabolically labeled sialoglyproteins were then biotinylated via the Staudinger ligation, and sialoglycopeptides containing azido-sialic acid glycans were immobilized to a solid support. The peptides linked to metabolically labeled sialylated glycans were then released from sialoglycopeptides and analyzed by mass spectrometry; in parallel, the glycans from azido-sialoglycoproteins were characterized by lectin microarrays. This method identified 75 unique N-glycosite-containing peptides from 55 different metabolically labeled sialoglycoproteins of which 42 were previously linked to cancer in the literature
Human samples. Skin fibroblasts were obtained with informed consent from the patients (P1.1 and P1.2) and healthy controls and cultured at 37.0°C under 5.0% CO2 in E199 culture medium, supplemented with 10% fetal calf serum and 1% penicillin/streptomycin. All cultures were negative for mycoplasma infection.. WES. The family was enrolled into the TIDEX gene discovery project (University of British Columbia IRB approval H12-00067), and provided written consent for the publication of this case report. WES of the subject and his unaffected parents was performed using the Agilent SureSelect Kit and Illumina HiSeq 2000 (PerkinElmer), covering 37.8× for the subject, 30.4× for the mother, and 27.5× for the father. The sequencing reads were mapped to the hg19 human reference genome. Rare variants, identified as those with minor allele frequency (,0.01), were assessed for predicted impact on protein function and screened under multiple inheritance models, as described previously (31). Sanger ...
W. Ledeen and R. K. ~ H-(-OAc I H-(-OAc I (H20Ac I Ph 3 == P-(-O(2 HS 00 XLVII y XLVIII I H-(-OH I AcNH-(-H I HO-(-H I H-(-OH I H-(-OH I (H20H HO-(-(OOH I (H2 I H-(-OH (HO I H-(-OAc I AcNH-(-H I AcO-(-H I H-(-OAc I H-(-OAc I (H 2 0Ac XLVI I AcNH-(-H I O-(-H I H-(-OH I H-(-OH I (H20H IX FIGURE 13. Sialic acid synthesis by the procedure of Mirzayanova et al. (1970). , 1957), resorcinol (Svennerholm, 1957b), dimethylaminobenzaldehyde (Werner and Odin, 1952), and thiobarbituric acid (Warren, 1959; Aminoff, 1959), but only a few of these are still in use. Although the various anomeric pairs could be differentiated by physical and enzymatic means, the precise structures remained uncertain until it was established that these reactions followed III HO, /H XXVIII OR /C:CC~n--r COOR HOCH2~ AcNH OH XXIX FIGURE 9. HO~~(COOH HOCH2 H,C-- OH AcNH 0 OR OH XXX General procedures for synthesis of l3-ketosides (XXIX) and a-ketosides (XXX) of sialic acids. Ketoside group Methyl Methyl Methyl Methyl n-Amyl n-Amyl ...
We identified CD169 as a specific binding-partner for exosomes in lymphoid tissue. Its restriction to subpopulations of macrophages dedicated to antigen capture and direct presentation and/or transfer of antigen to other APCs17 suggests an important role of CD169-mediated capture in the immune response to exosomal antigen. CD169+ macrophages are strategically situated at antigen entry points into the spleen and LN and are involved in capture of sialylated pathogens13,30; however, a role for CD169 in capturing exosomes has not previously been demonstrated.. Despite the low affinity (Kd ∼ 1.4 mM) of CD169 for α2,3-linked sialic acid,33 we observed abundant binding of exosomes to this receptor. This may be a result of high level expression of α2,3-linked sialic acid expression on exosomes, as linear increases in CD169 ligand availability results in logarithmic increases in avidity.13 Moreover, due to their size, exosomes may suffer less shear stress, therefore gaining greater access to ...
Wiley Online Library is migrating to a new platform powered by Atypon, the leading provider of scholarly publishing platforms. The new Wiley Online Library will be migrated over the weekend of February 24 and 25 and will be live on February 26, 2018. For more information, please visit our migration page:http://www.wileyactual.com/WOLMigration ...
4AMA: Structural Insights Into the Recovery of Aldolase Activity in N-Acetylneuraminic Acid Lyase by Replacement of the Catalytically Active Lysine with Gamma-Thialysine by Using a Chemical Mutagenesis Strategy.
4AHO: Structural Insights Into the Recovery of Aldolase Activity in N-Acetylneuraminic Acid Lyase by Replacement of the Catalytically Active Lysine with Gamma-Thialysine by Using a Chemical Mutagenesis Strategy.
Alpha1?acid glycoprotein (AGP) is a highly glycosylated lipid?binding acute?phaseprotein. Although the exact mechanisms are unknown, several studies havesuggested that AGP may regulate the function of neutrophils and hence modulateinflammatory responses. The general aim of this thesis was to investigate if AGP isable to mediate intracellular signalling in neutrophils through binding to specificreceptors.Measurements of intracellular calcium concentration showed that AGP elicited asmall rise in [Ca2+]i in neutrophils that was markedly enhanced by pre?treatmentwith anti?L?selectin antibodies. In contrast, desialylation of AGP reduced the Ca2+mobilizing capacity significantly. The AGP?induced Ca2+ signal was mediatedthrough Src tyrosine kinases, PLC and PI3K which suggests involvement of cellsurface receptors. Indeed, AGP was shown to bind to, and mediate Ca2+ signallingthrough, sialic acid binding immunoglobulin?like lectin (Siglec)?5 and/or ?14.Increased fucosylation of AGP is common during ...
J:188842 Bergfeld AK, et al., Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid. J Biol Chem. 2012 Aug 17;287(34):28865-81 ...
... di- ets - investasi dana forex, monitoring forex broker, forex analyst report, trading system in forex, happy forex 1 5
In previous studies, we have shown that the highly sialylated renal anti-adhesin Podocalyxin (PC) is expressed in the developing brain [13], [14 ...
UFC President Dana White talked about Ronda Rousey's impressive performance at UFC 190 on the post-fight show. White was all praise for Rousey's 34 second dismantling of Bethe Correia, and called her "the most badass woman anyone has ever seen." "You don't see women knock women out like that," White said. "She knocked her out. She's an absolute beast. She j
DANA BUD A U Manua deII s tr a I de VALERI MOlUOV D\HR/" dintr-un lrnsns sentiment de regret Cal1E nu are ...
Check out the UFC 216 Ultimate Media Day face-offs where UFC president Dana White was on guard, even though Tony Ferguson snuck it a little ...
Creating a World of Change with Karlyn Emile 05, 11 Author: Dana Ratliff https://fiualumni.com/2016/07/27/creating-a-world-of-change-with-karlyn-emile-05-11/
At The Natural Path Center for Wellness we help our patients overcome and cope with a wide variety of health concerns. Below are some of the more common issues we assist people with ...
Direct OE replacement Jeep parts and accessories built to the original specifications by Omix-ADA. Limited five year manufacturers warranty.
All of u - Stop answering questions. Instead ask me questions so I can answer. . All of u - ... | Page: 89 | 1630551 | Geet - Hui Sabse Parayee Forum
Burdová Kateřina; Supervisor: Ralbovská Dana Rebeka; Opponent: Knezović Renata (České vysoké učení technické v Praze. Vypočetní a informační centrum., 2013-06-25) ...
We present a comprehensive report of two siblings with hereditary inclusion body myopathy (HIBM). The clinical features and histological characteristics of the muscle biopsies showed the typical pattern of predominantly distal vacuolar myopathy with quadriceps sparing. This was confirmed by muscle MRI. PNA lectin staining showed an increased signal at the sarcolemma in patient muscle sections compared to control muscle, indicating reduced sialylation of glycoconjugates. Mutation analysis revealed compound heterozygous mutations in the GNE gene, encoding the key enzyme in sialic acid synthesis UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase: a missense mutation (c.2086G > A; p.V696M) previously described in HIBM patients of Indian origin, and a novel frame shift mutation (c.1295delA; p.K432RfsX17) leading to a premature stopcodon. These findings confirmed the diagnosis of HIBM on the histological, molecular and biochemical level ...
Definition of Salla disease in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Salla disease? Meaning of Salla disease as a legal term. What does Salla disease mean in law?
Alkaline borohydride-reduced keratan sulphate (KS) chains derived from bovine femoral head cartilage were fractionated by lectin affinity chromatography with Sambucus nigra agglutinin (SNA) into binding and non-binding populations. Analysis of the SNA-binding and non-binding KS chains using 600 MHz 1H n.m.r. spectroscopy showed that the former population contained alpha(2-6)-N-acetylneuraminic acid residues and the latter contained primarily alpha(2-3)-N-acetylneuraminic acid residues as chain terminators. Both populations contained a similar proportion of alpha(2-3)-N-acetylneuraminic acid residues within their protein-linkage regions, and similar sulphation and fucosylation levels. Analysis of these two fractions by gel-permeation chromatography (g.p.c.) on a TSK-30 XL column showed them to have the same size distributions. It was concluded from the n.m.r. spectra and g.p.c. data that the populations differed primarily in the mode of linkage of the chain-terminating sialic acids. ...
Poly-α2,8-sialic acid (PSA) has been implicated in numerous normal and pathological processes, including development, neuronal plasticity, and tumor metastasis. We report that cell surface PSA expression can be reversibly inhibited by a small molecule,N-butanoylmannosamine (ManBut). Inhibition occurs through a metabolic mechanism in which ManBut is converted to unnatural sialic acid derivatives that effectively act as chain terminators during cellular PSA biosynthesis. N-Propanoylmannosamine (ManProp), which differs from ManBut by a single methylene group, did not inhibit PSA biosynthesis. Modulation of PSA expression by chemical means has a role complementary to genetic and biochemical approaches in the study of complex PSA-mediated events. ...
Sialic-acid-binding immunoglobulin-like lectins (Siglecs) are members of the Ig superfamily that bind sialic acids in different linkages in a wide variety of glycoconjugates. These membrane receptors are expressed in a highly specific manner, predominantly within the haematopoietic system. The CD33- …
Sialic acid storage disease, also known as Salla disease, is a lysosomal storage disorder caused by defective transport of lysosomal degradation product, free sialic acid, across the lysosomal membrane. Functional consequences come only from central nervous system presenting slowly progressive mental retardation associated with ataxia and some other neurological manifestations. Affected infants present first signs of the disease already at 3 to 9 months of age as muscular hypotonia, truncal and limb ataxia, often transient nystagmus and delayed motor development. Developmental profile of SD patients is characterized by slowly progressive general handicap with motor performance more severely and earlier affected than cognitive skills. All patients are severely mentally retarded from third decade on. Life span of affected patients is close to normal.
Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
or. symbol for a residue (or molecule) of N‐acetylneuraminic acid (i.e. sialic acid). It accumulates in lysosomes in Salla disease and infantile sialic acid storage disease, and in cell cytosol in sialuria.. [...] ...
• Serum hepatic cell-surface enzymes, isoenzymes, and sialic acid levels may be useful adjuncts in detecting early metastatic disease and in evaluating the tumo
The study of norovirus entry has been particularly hampered by the lack of an efficient cell culture system. MNV is the only norovirus that replicates well in cell culture and therefore lends itself to a detailed molecular analysis of norovirus entry. In this study, we focused on the identification of molecules involved in MNV-1 attachment to cells. Very recently, sialylated neoglycoproteins like sialyl-Lewis x were identified as an additional HuNoV attachment factor (44). FCV, another member of the Caliciviridae family, attaches to α2,6-linked SA and uses JAM-1 as a receptor (31, 53). Many viruses, including rotaviruses, also use terminal SA as attachment receptors for infection. Therefore, we investigated the role of SA and gangliosides, SA-containing lipids, during the MNV-1 binding and infection of permissive murine macrophages.. Two main conclusions can be drawn from the data presented in this study. First, SA plays an important role during the MNV-1 infection of murine macrophages. Lectin ...