TY - JOUR. T1 - Donor substrate promiscuity of bacterial β1-3-N-acetylglucosaminyltransferases and acceptor substrate flexibility of β1-4-galactosyltransferases. AU - Li, Yanhong. AU - Xue, Mengyang. AU - Sheng, Xue. AU - Yu, Hai. AU - Zeng, Jie. AU - Thon, Vireak. AU - Chen, Yi. AU - Muthana, Musleh M.. AU - Wang, Peng G.. AU - Chen, Xi. PY - 2016/4/15. Y1 - 2016/4/15. N2 - β1-3-N-Acetylglucosaminyltransferases (β3GlcNAcTs) and β1-4-galactosyltransferases (β4GalTs) have been broadly used in enzymatic synthesis of N-acetyllactosamine (LacNAc)-containing oligosaccharides and glycoconjugates including poly-LacNAc, and lacto-N-neotetraose (LNnT) found in the milk of human and other mammals. In order to explore oligosaccharides and derivatives that can be synthesized by the combination of β3GlcNAcTs and β4GalTs, donor substrate specificity studies of two bacterial β3GlcNAcTs from Helicobacter pylori (Hpβ3GlcNAcT) and Neisseria meningitidis (NmLgtA), respectively, using a library of 39 ...

Looking for the definition of n-acetylglucosaminyltransferases? Find out what is the full meaning of n-acetylglucosaminyltransferases on Abbreviations.com! The Webs largest and most authoritative acronyms and abbreviations resource.

The alteration of glucose metabolism, through increased uptake of glucose and glutamine addiction, is essential to cancer cell growth and invasion. Increased flux of glucose through the Hexosamine Biosynthetic Pathway (HBP) drives increased cellular O-GlcNAcylation (hyper-O-GlcNAcylation) and contributes to cancer progression by regulating key oncogenes. However, the association between hyper-O-GlcNAcylation and activation of these oncogenes remains poorly characterized. Here, we implement a qualitative modeling framework to analyze the role of the Biological Regulatory Network in HBP activation and its potential effects on key oncogenes. Experimental observations are encoded in a temporal language format and model checking is applied to infer the model parameters and qualitative model construction. Using this model, we discover step-wise genetic alterations that promote cancer development and invasion due to an increase in glycolytic flux, and reveal critical trajectories involved in cancer progression

TY - JOUR. T1 - Transcriptional regulation of N-acetylglucosaminyltransferase V by the src oncogene. AU - Buckhaults, Phillip. AU - Chen, Lin. AU - Fregien, Nevis. AU - Pierce, Michael. PY - 1997/8/1. Y1 - 1997/8/1. N2 - Transformation of baby hamster kidney fibroblasts by the Rous sarcoma virus causes a significant increase in the GlcNAcβ(1,6)Man-branched oligosaccharides by elevating the activity and mRNA transcript levels encoding N-acetylglucosaminyltransferase V (GlcNAc-T V). Elevated activity and mRNA levels could be inhibited by blocking cell proliferation with herbimycin A, demonstrating that Src kinase activity can regulate GlcNAc-T V expression. 5 RACE analysis was used to identify a 3-kilobase 5- untranslated region from GlcNAc-T V mRNA and locate a transcriptional start site in a 25-kilobase pair GlcNAc-T V human genomic clone. A 6-kilobase pair fragment of the 5 region of the gene contained AP-1 and PEA3/Ets binding elements and, when co-transfected with a src expression plasmid ...

This gene encodes an enzyme that acts in the lumen of the endoplasmic reticulum to catalyze the transfer of N-acetylglucosamine to serine or threonine residues of extracellular-targeted proteins. This enzyme modifies proteins containing eukaryotic growth factor (EGF)-like domains, including the Notch receptor, thereby regulating developmental signalling. Mutations in this gene have been observed in individuals with Adams-Oliver syndrome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015 ...

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Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).

Shop Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase ELISA Kit, Recombinant Protein and Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.

In the present study, we demonstrate that GnT-V expression is decreased or lost in about half of NSCLCs, although GnT-V is expressed in bronchial epithelial cells, bronchial gland cells, and alveolar pneumocytes. Histology was significantly correlated with GnT-V expression; low GnT-V expression was more frequently found in squamous cell carcinomas than in non-squamous cell carcinomas. Furthermore, low GnT-V expression was associated with a shorter survival period and was an unfavorable prognostic factor in pStage I resected non-squamous cell carcinomas.. GnT-V expression is not equal to the expression of β1-6 branching asparagine-linked oligosaccharides analyzed by L-PHA histochemistry, because (a) GnT-V has been shown to have a function as an inducer of angiogenesis (26) that is a completely different function from the original function of glycosyltransferase, and (b) GnT-V expression does not necessarily result in the synthesis of β1-6 branching oligosaccharides, depending on the cell and ...

Alterations in chromatin modulators like the nuclear deubiquitinating enzyme BAP1 are the most frequently observed genetic alterations reported in intrahepatic CCA, yet the molecular mechanisms by which they modulate cancer cell behavior are unknown. BAP1 can act as a tumor suppressor and can regulate several cellular processes through its interaction with other protein partners such as host cell factor 1, O-linked N-acetylglucosamine transferase, transcription factor Ying Yang1, ASXL1/2, and FoxK1/K2 and DNA repair proteins like BRCA1/BARD1 heterodimer and RAD51. In the present study, we identify alterations in long non-coding RNA gene expression as a contributor to tumor cell phenotype and differential therapeutic sensitivity of CCA cells that is related to BAP1 expression.. Alterations in BAP1 expression in other cancers such as renal cell carcinoma, breast carcinoma, small cell and non-small cell lung cancers, malignant mesothelioma, metastasizing uveal melanoma, and hepatic cancers can ...

5GGK: Crystal structure of N-terminal domain of human protein O-mannose beta-1,2-N-acetylglucosaminyltransferase in complex with Man-beta-pNP

K03857 PIGA; phosphatidylinositol N-acetylglucosaminyltransferase subunit A [EC:2.4.1.198] K03859 PIGC; phosphatidylinositol N-acetylglucosaminyltransferase subunit C K03858 PIGH; phosphatidylinositol N-acetylglucosaminyltransferase subunit H K03858 PIGH; phosphatidylinositol N-acetylglucosaminyltransferase subunit H K03861 PIGP; phosphatidylinositol N-acetylglucosaminyltransferase subunit P K03860 PIGQ; phosphatidylinositol N-acetylglucosaminyltransferase subunit Q K11001 PIGY; phosphatidylinositol N-acetylglucosaminyltransferase subunit Y K11001 PIGY; phosphatidylinositol N-acetylglucosaminyltransferase subunit Y K09658 DPM2; dolichol phosphate-mannose biosynthesis regulatory protein K09658 DPM2; dolichol phosphate-mannose biosynthesis regulatory protein K03434 PIGL; N-acetylglucosaminylphosphatidylinositol deacetylase [EC:3.5.1.89] K05283 PIGW; glucosaminylphosphatidylinositol acyltransferase [EC:2.3.-.-] K01127 GPLD1; glycosylphosphatidylinositol phospholipase D [EC:3.1.4.50] K05284 PIGM; ...

TY - JOUR. T1 - A dual approach for improving homogeneity of a human-type N-glycan structure in Saccharomyces cerevisiae. AU - Piirainen, Mari. AU - Boer, Harry. AU - de Ruijter, Jorg C.. AU - Frey, Alexander D.. PY - 2016. Y1 - 2016. N2 - N-glycosylation is an important feature of therapeutic and other industrially relevant proteins, and engineering of the N-glycosylation pathway provides opportunities for developing alternative, non-mammalian glycoprotein expression systems. Among yeasts, Saccharomyces cerevisiae is the most established host organism used in therapeutic protein production and therefore an interesting host for glycoengineering. In this work, we present further improvements in the humanization of the N-glycans in a recently developed S. cerevisiae strain. In this strain, a tailored trimannosyl lipid-linked oligosaccharide is formed and transferred to the protein, followed by complex-type glycan formation by Golgi apparatus-targeted human N-acetylglucosamine transferases. We ...

SWISS-MODEL Repository entry for A1WC06 (MURG_ACISJ), UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase. Acidovorax sp (strain JS42)

The present study provides experimental evidence for the function of Dbv4 as a DNA-binding protein which acts as a positive regulator of A40926 biosynthesis by controlling expression of two operons of the dbv cluster: the dbv14-dbv8 operon, encoding the four cross-linking oxygenases, the halogenase, the N-acetylglucosamine transferase and the N-acylase, and the dbv30-dbv35 operon, encoding the four enzymes involved in DPG biosynthesis (5, 25), as well as the sodium-proton antiporter and a protein of unknown function. In the present study, we demonstrate that the expression of dbv4 and of the dbv14-dbv8 and dbv30-dbv35 operons is negatively influenced by phosphate by RT-PCR analysis and real-time RT-PCR experiments (Fig. 3 and 4) and that in vitro Dbv4 binds to the region upstream of dbv14 and dbv30 (Fig. 6).. In vitro, Dbv4 and its ortholog Bbr were found to equally bind four distinct regions: the 171-bp fragment containing the dbv14 promoter (Fig. 6C), the 351-bp fragment upstream to dbv30 ...

TY - JOUR. T1 - Inhibition of O-GlcNAc transferase activity reprograms prostate cancer cell metabolism. AU - Itkonen, Harri M. AU - Gorad, Saurabh S. AU - Duveau, Damien Y. AU - Martin, Sara E S. AU - Barkovskaya, Anna. AU - Bathen, Tone F. AU - Moestue, Siver A. AU - Mills, Ian G. PY - 2016/1/27. Y1 - 2016/1/27. N2 - Metabolic networks are highly connected and complex, but a single enzyme, O-GlcNAc transferase (OGT) can sense the availability of metabolites and also modify target proteins. We show that inhibition of OGT activity inhibits the proliferation of prostate cancer cells, leads to sustained loss of c-MYC and suppresses the expression of CDK1, elevated expression of which predicts prostate cancer recurrence (p=0.00179). Metabolic profiling revealed decreased glucose consumption and lactate production after OGT inhibition. This decreased glycolytic activity specifically sensitized prostate cancer cells, but not cells representing normal prostate epithelium, to inhibitors of oxidative ...

Pomgnt1 (untagged) - Mouse protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 1, (10ug), 10 µg.

Mucopolysaccharidosis (MPS) I is a lysosomal storage disease caused by a deficiency of α-L-iduronidase (IDUA) (EC 3.2.1.76); enzyme replacement therapy is the conventional treatment for this genetic disease. Arabidopsis cgl mutants are characterized by a deficiency of the activity of N-acetylglucosaminyl transferase I (EC 2.4.1.101), the first enzyme in the pathway of hybrid and complex N-glycan biosynthesis. To develop a seed-based platform for the production of recombinant IDUA for potential treatment of MPS I, cgl mutant seeds were generated to express human IDUA at high yields and to avoid maturation of the N-linked glycans on the recombinant human enzyme. Enzyme kinetic data showed that cgl-IDUA has similar enzymatic properties to the commercial recombinant IDUA derived from cultured Chinese hamster ovary (CHO) cells (AldurazymeTM). The N-glycan profile showed that cgl-derived IDUA contained predominantly high-mannose-type N-glycans (94.5%), and the residual complex/hybrid ...

Myc-DDK-tagged ORF clone of Homo sapiens UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2) as transfection-ready DNA - 10 µg - OriGene - cdna clones

The GRC prioritizes curation efforts that improve gene representation in the human reference genome assembly. In some cases, such curation takes the form of base-pair level edits. The recent GRCh38.p11 patch release includes a new, curated, representation for the GCNT2 gene. The representation of the GCNT2 gene in the GRCh38 reference assembly contains the C allele for SNP rs539351 on chromosome 6 (NC_000006.12) at position 10,586,805, which reflects the sequence from the underlying component AL358777.12 (RP11-421M1) (Figure 1, top). During human development, the fetal blood group antigen (i) is converted to the adult antigen (I) by a beta-1,6-N-acetylglucosaminyltransferase-2 (GCNT2). Alternative splicing of the gene generates 3 isoforms, which differ only in their first exon. The SNP rs539351 is found in the first exon unique to the GCNT2 isoform C, which is the only one expressed in red blood cells, where this conversion occurs (NM_145655.3: c.816C,G (NP_663630.2: p.Asp272Glu). A user ...

The GRC prioritizes curation efforts that improve gene representation in the human reference genome assembly. In some cases, such curation takes the form of base-pair level edits. The recent GRCh38.p11 patch release includes a new, curated, representation for the GCNT2 gene. The representation of the GCNT2 gene in the GRCh38 reference assembly contains the C allele for SNP rs539351 on chromosome 6 (NC_000006.12) at position 10,586,805, which reflects the sequence from the underlying component AL358777.12 (RP11-421M1) (Figure 1, top). During human development, the fetal blood group antigen (i) is converted to the adult antigen (I) by a beta-1,6-N-acetylglucosaminyltransferase-2 (GCNT2). Alternative splicing of the gene generates 3 isoforms, which differ only in their first exon. The SNP rs539351 is found in the first exon unique to the GCNT2 isoform C, which is the only one expressed in red blood cells, where this conversion occurs (NM_145655.3: c.816C,G (NP_663630.2: p.Asp272Glu). A user ...

Mgat4c (untagged) - Mouse mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative) (Mgat4c), transcript variant 1, (10ug), 10 µg.

View mouse Pomgnt2 Chr9:121981606-121996053 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression

View mouse Pomgnt1 Chr4:116123840-116159849 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression

Complete information for MGAT5 gene (Protein Coding), Alpha-1,6-Mannosylglycoprotein 6-Beta-N-Acetylglucosaminyltransferase, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

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PRK12446 (PSSM ID: 171505): Conserved Protein Domain Family PRK12446, undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase; Reviewed

Complete information for LFNG gene (Protein Coding), LFNG O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

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putative UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase LOC402377-like; K03766 beta-1,3-N-acetylglucosaminyltransferase 5 [EC:2.4.1.206] ...

Necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.

β-1,3-galactosyl-O-glycosyl-glycoprotein β-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.102); N-acetyllactosaminide β-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.150); protein O-β-xylosyltransferase (EC 2.4.2.26); UDP-GlcA:arabinogalactan β-glucuronosyltransferase (EC 2.4.1.- ...

List of all the English words with 29 letters containing letter A. acetylglucosaminyltransferase, acetylmannosaminyltransferase, acylphosphatidylethanolamines, alkylacetylglycerophosphatase, aminocyclopropanecarboxylates, antidisestablishmentarianisms

As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.

Order GCNT1 ELISA Kits for many Reactivities. Chicken, Cow, Dog and more. Compare GCNT1 ELISA Kits and find the right product on antibodies-online.com.

B3gnt7 - B3gnt7 (untagged ORF) - Rat UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7 (B3gnt7), (10 ug) available for purchase from OriGene - Your Gene Company.

Supplier pricing and chemical structure of high purity Man(a1-2)-Man(a1-2)-Man(a1-3)-[Man(a1-6)-Man(a1-6)]-Man(b1-4)-GlcNAc(b1-4)-GlcNAc from Omicron Biochemicals, Inc.

Supplier pricing and chemical structure of high purity Man(a1-2)-Man(a1-3)-[Man(a1-6)-Man(a1-6)]-Man(b1-4)-GlcNAc(b1-4)-GlcNAc from Omicron Biochemicals, Inc.

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The MGAT5B gene encodes a beta-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.155) that functions in the synthesis of complex cell surface N-glycans (Kaneko et al., 2003 [PubMed 14623122]).[supplied by OMIM, Nov 2008 ...

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This gene is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the MFNG (GeneID: 4242) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. [provided by RefSeq, May 2018 ...

K00790 murA; UDP-N-acetylglucosamine 1-carboxyvinyltransferase [EC:2.5.1.7] K00075 murB; UDP-N-acetylmuramate dehydrogenase [EC:1.3.1.98] K01924 murC; UDP-N-acetylmuramate--alanine ligase [EC:6.3.2.8] K01924 murC; UDP-N-acetylmuramate--alanine ligase [EC:6.3.2.8] K01925 murD; UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC:6.3.2.9] K01928 murE; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase [EC:6.3.2.13] K01929 murF; UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [EC:6.3.2.10] K01921 ddl; D-alanine-D-alanine ligase [EC:6.3.2.4] K01775 alr; alanine racemase [EC:5.1.1.1] K01776 murI; glutamate racemase [EC:5.1.1.3] K01297 ldcA; muramoyltetrapeptide carboxypeptidase [EC:3.4.17.13] K01000 mraY; phospho-N-acetylmuramoyl-pentapeptide-transferase [EC:2.7.8.13] K02563 murG; UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [EC:2.4.1.227] K06153 bacA; undecaprenyl-diphosphatase [EC:3.6.1.27] K19302 ...

SWISS-MODEL Repository entry for Q3Z5R9 (MURG_SHISS), UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase. Shigella sonnei (strain Ss046)

Hereditary multiple exostoses (EXT; MIM 133700) is an autosomal dominant bone disorder. It is genetically heterogeneous with at least three chromosomal loci: EXT1 on 8q24.1, EXT2 on 11p11, and EXT3 on 19p. EXT1 and EXT2, the two genes responsible for EXT1 and EXT2, respectively, have been cloned. Recently, three other members of the EXT gene family, named the EXT-like genes (EXTL: EXTL1, EXTL2, and EXTL3), have been isolated. EXT1, EXT2, and the three EXTLs are homologous with one another. We have identified the intron-exon boundaries of EXTL1 and EXTL3 and analyzed EXT1, EXT2, EXTL1, and EXTL3, in 36 Chinese families with EXT, to identify underlying disease-related mutations in the Chinese population. Of the 36 families, five and 12 family groups have mutations in EXT1 and EXT2, respectively. No disease-related mutation has been found in either EXTL1 or EXTL2, although one polymorphism has been detected in EXTL1. Of the 15 different mutations (three families share a common mutation in EXT2), 12 ...

In order for a protein modification to play an active role in signal transduction, it needs to have certain key features. First, the modification needs to be dynamic. For the proteins that have been examined to date, the O-GlcNAc half-life is much shorter than that of the modified polypeptide chain (8). Second, the removal or attachment of the modification should be inducible by certain stimuli. O-GlcNAc modification of certain proteins is known to change in response to T cell activation, insulin signaling, glucose metabolism, and cell cycle progression (6). Thus, O-GlcNAc displays features essential for a role in signal transduction.. Consistent with O-GlcNAc being dynamic and inducible, regulated nucleocytoplasmic enzymes for the attachment [O-GlcNAc transferase (OGT)] and for the removal (O-GlcNAcase) of the modification have been purified, characterized, and cloned (9-12). The OGT enzyme is modified by both O-GlcNAc and tyrosine phosphorylation and has 11 protein-protein interaction domains ...

Fingerprint Dive into the research topics of Dynamic O-GlcNAc modification of nucleocytoplasmic proteins in response to stress: A survival response of mammalian cells. Together they form a unique fingerprint. ...

TY - JOUR. T1 - Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including α-actinin 4. AU - Akimoto, Yoshihiro. AU - Miura, Yuri. AU - Toda, Tosifusa. AU - Wolfert, Margreet A.. AU - Wells, Lance. AU - Boons, Geert Jan. AU - Hart, Gerald Warren. AU - Endo, Tamao. AU - Kawakami, Hayato. PY - 2011. Y1 - 2011. N2 - Purpose. The objective of the present study is to identify proteins that change in the extent of the modification with O-linked N-acetylglucosamine (O-GlcNAcylation) in the kidney from diabetic model Goto-Kakizaki (GK) rats, and to discuss the relation between O-GlcNAcylation and the pathological condition in diabetes. Methods. O-GlcNAcylated proteins were identified by two-dimensional gel electrophoresis, immunoblotting and peptide mass fingerprinting. The level of O-GlcNAcylation of these proteins was examined by immunoprecipitation, immunoblotting and in situ Proximity Ligation Assay (PLA). Results: O-GlcNAcylated ...

The O-GlcNAc (O-linked N-acetylglucosamine) modification is a dynamic and reversible form of protein glycosylation occurring on specific serine and threonine residues of intracellular proteins [1,2]. Since the initial discovery of O-GlcNAc [3], technological advances have greatly facilitated its detection, and proteomics studies [4-6] have shown that a significant proportion of cellular proteins are O-GlcNAcylated. However, the functional importance of O-GlcNAc is only just emerging, with evidence to suggest that it may regulate protein activity in a manner analogous (and complementary) to phosphorylation [7]. O-GlcNAc levels are known to respond dynamically to nutrient availability [1] and stress [8], and to undergo changes during the cell cycle [9] and development [10]. O-GlcNAc has been shown to be associated with a range of human diseases [2]. Strikingly, only two enzymes orchestrate the O-GlcNAc modification. Both the OGT (O-GlcNAc transferase) and its antagonistic OGA (O-GlcNAcase or ...

TY - JOUR. T1 - The lineage stability and suppressive program of regulatory T cells require protein O-GlcNAcylation. AU - Liu, Bing. AU - Salgado, Oscar C.. AU - Singh, Sangya. AU - Hippen, Keli L.. AU - Maynard, Jason C.. AU - Burlingame, Alma L.. AU - Ball, Lauren E.. AU - Blazar, Bruce R.. AU - Farrar, Michael A.. AU - Hogquist, Kristin A.. AU - Ruan, Hai Bin. PY - 2019/12/1. Y1 - 2019/12/1. N2 - Regulatory T (Treg) cells control self-tolerance, inflammatory responses and tissue homeostasis. In mature Treg cells, continued expression of FOXP3 maintains lineage identity, while T cell receptor (TCR) signaling and interleukin-2 (IL-2)/STAT5 activation support the suppressive effector function of Treg cells, but how these regulators synergize to control Treg cell homeostasis and function remains unclear. Here we show that TCR-activated posttranslational modification by O-linked N-Acetylglucosamine (O-GlcNAc) stabilizes FOXP3 and activates STAT5, thus integrating these critical signaling pathways. ...

The hexosamine biosynthetic pathway (HBP) generates the substrate for the O-linked β-N-acetylglucosamine (O-GlcNAc) modification of proteins. The HBP also serves as a stress sensor and has been reported to be involved with nuclear factor of activated T-cells (NFAT) activation, which can contribute to multiple cellular processes including cell metabolism, proliferation, and inflammation. In our previously published report, Fibroblast Growth Factor (FGF) 23, an important endocrine pro-inflammatory mediator, was shown to activate the FGFR4/phospholipase Cγ (PLCγ)/nuclear factor of activated T-cells (NFAT) signaling in chronic inflammatory airway diseases such as cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD). Here, we demonstrate that FGF23 increased the O-GlcNAc modification of proteins in HBECs. Furthermore, the increase in O-GlcNAc levels by FGF23 stimulation resulted in the downstream activation of NFAT and secretion of interleukin-6 (IL-6). Conversely, inhibition of FGF23

N-acetyllactosaminide β-1,3-N-acetylglucosaminyltransferase (EC 2.4.1.149); Glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase (EC 2.4.1.122); fucose-specific β-1,3-N-acetylglucosaminyltransferase (EC 2.4.1.-); globotriosylceramide β-1,3-GalNAc transferase (EC 2.4.1.79); chondroitin synthase (β-1,3-GlcUA and β-1,4-GalNAc transferase (EC 2.4.1.175); chondroitin β-1,3-glucuronyltransferase (EC 2.4.1.226); chondroitin β-1,4-N-acetylgalactosaminyltransferase (EC 2.4.1.-); UDP-Gal: β-galactosylxylosylprotein β-1,3-galactosyltransferase (EC 2.4.1.134); UDP-GlcNAc: O-fucosylpeptide β-1,3-N-acetylglucosaminyltransferase (EC 2.4.1.222 ...

TY - JOUR. T1 - Profiling of Protein O-GlcNAcylation in Murine CD8+ Effector- and Memory-like T Cells. AU - Lopez Aguilar, Aime. AU - Gao, Yu. AU - Hou, Xiaomeng. AU - Lauvau, Gregoire. AU - Yates, John R.. AU - Wu, Peng. N1 - Funding Information: This work was performed at The Scripps Research Institute with the financial support from the National Institutes of Health to P.W. (GM093282, GM113046) and to J.R.Y. (MH067880, GM103533). We thank the Histology Core Facility at TSRI for providing access to their equipment and support.. PY - 2017/12/15. Y1 - 2017/12/15. N2 - During an acute infection, antigenic stimulation leads to activation, expansion, and differentiation of naïve CD8+ T cells, first into cytotoxic effector cells and eventually into long-lived memory cells. T cell antigen receptors (TCRs) detect antigens on antigen-presenting cells (APCs) in the form of antigenic peptides bound to major histocompatibility complex I (MHC-I)-encoded molecules and initiate TCR signal transduction ...

Abstract: O-GlcNAc transferase (OGT) glycosylates a diverse range of intracellular proteins with O-linked N-acetylglucosamine (O-GlcNAc), an essential and dynamic post-translational modification in metazoans. Although this enzyme modifies hundreds of proteins with O-GlcNAc, it is not understood how OGT achieves substrate specificity. In this study, we describe the application of a high-throughput OGT assay to a library of peptides. We mapped sites of O-GlcNAc modification by electron transfer dissociation MS and found that they correlate with previously detected O-GlcNAc sites. Crystal structures of four acceptor peptides in complex with Homo sapiens OGT suggest that a combination of size and conformational restriction defines sequence specificity in the −3 to +2 subsites. This work reveals that although the N-terminal TPR repeats of OGT may have roles in substrate recognition, the sequence restriction imposed by the peptide-binding site makes a substantial contribution to O-GlcNAc site ...

Beta-(1,3)-N-Acetylglucosaminyltransferase Size: 100 mU Introduction: B3GlcNAcT is a β-1,3-N-acetylglucosaminyltransferase that transfers glucosamine from UDP

Cancer cells increase nutrient consumption leading to the altered metabolic state known as the Warburg effect. One pathway dependent on glucose, glutamine and acetyl-CoA is the Hexosamine Biosynthetic Pathway (HBP). Increased flux through the HBP leads to elevated post-translation addition of O-linked-β-N-acetylglucosamine (O-GlcNAc) on a diverse population of nuclear and cytosolic proteins, many of which are implicated in cancer. Recently, our lab provided the first evidence that breast and prostate cancers increases total O-GlcNAcylation by increasing O-GlcNAc Transferase (OGT) levels. Importantly, reducing OGT activity inhibits cancer cell invasion in vitro and metastasis in vivo. OGT inhibition reduces breast and prostate cancer cell invasion through, in part, inhibition of the oncogenic transcription factor, FOXM1 and its transcriptional target matrix metalloproteinase 2 (MMP2). Here, we show that OGT regulation of FOXM1 and cancer cell invasion requires regulation of the NAD+-dependent ...

Description: This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins ...

O-GlcNAc transferase antibody [GT2037] (O-linked N-acetylglucosamine (GlcNAc) transferase) for WB. Anti-O-GlcNAc transferase mAb (GTX629813) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.

diff --git a/fs/ext4/ext4_jbd2.c b/fs/ext4/ext4_jbd2.c index e8287f4..eb88564 100644 --- a/fs/ext4/ext4_jbd2.c +++ b/fs/ext4/ext4_jbd2.c @@ -23,6 +23,7 @@ int __ext4_handle_get_bitmap_access(const char *where, unsigned int line, if (err) ext4_journal_abort_handle(where, line, __func__, bh, handle, err); + ext4_journal_trace(SNAP_DEBUG, where, handle, 1); } return err; } @@ -40,6 +41,7 @@ int __ext4_journal_get_write_access_inode(const char *where, unsigned int line, if (err) ext4_journal_abort_handle(where, line, __func__, bh, handle, err); + ext4_journal_trace(SNAP_DEBUG, where, handle, 1); } return err; } @@ -91,6 +93,7 @@ int __ext4_forget(const char *where, unsigned int line, handle_t *handle, if (err) ext4_journal_abort_handle(where, line, __func__, bh, handle, err); + ext4_journal_trace(SNAP_DEBUG, where, handle, 1); return err; } return 0; @@ -108,6 +111,7 @@ int __ext4_forget(const char *where, unsigned int line, handle_t *handle, error %d when attempting revoke, err); } ...

O-GlcNAcylation is the addition of β-D-N-acetylglucosamine to serine or threonine residues of nuclear and cytoplasmic proteins. O-linked N-acetylglucosamine (O-GlcNAc) was not discovered until the early 1980s and still remains difficult to detect and quantify. Nonetheless, O-GlcNAc is highly abundan …

doc::unibi_get_ext_bool_name(3) unibilium doc::unibi_get_ext_bool_name(3) NAME unibi_get_ext_bool_name, unibi_set_ext_bool_name, unibi_get_ext_num_name, unibi_set_ext_num_name, unibi_get_ext_str_name, unibi_set_ext_str_name - access the names of extended capabilities of a terminal object SYNOPSIS #include ,unibilium.h, const char *unibi_get_ext_bool_name(const unibi_term *ut, size_t i); const char *unibi_get_ext_num_name(const unibi_term *ut, size_t i); const char *unibi_get_ext_str_name(const unibi_term *ut, size_t i); void unibi_set_ext_bool_name(unibi_term *ut, size_t i, const char *s); void unibi_set_ext_num_name(unibi_term *ut, size_t i, const char *s); void unibi_set_ext_str_name(unibi_term *ut, size_t i, const char *s); DESCRIPTION Get/set the names of extended boolean, numeric, and string capabilities. i is the index of the extended capability to act on; it must be less than unibi_count_ext_bool(ut), unibi_count_ext_num(ut), or unibi_count_ext_str(ut), respectively. Note that ...

This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase protein family. The encoded enzyme is involved in the biosynthesis of poly-N-acetyllactosamine chains and prefers lacto-N-neotetraose as a substrate. It is a type II transmembrane protein ...

Protein glycosylation is usually relegated to the cell surface and intracellular compartments. In a fascinating exception to this rule that was first observed in the 1980s, A GlcNAc monosaccharide can be added to serine and threonine residues of cytosolic proteins. Many labs are trying to understand the dynamic regulation of the addition and removal of this sugar that seemingly has a hand in every cellular process and disease state known to man. More and more examples are being found to suggest that this modification and phosphorylation regulate each other, as if they werent already complicated enough on their own.. There are a handful of ways to detect O-GlcNAc, which have helped build the laundry list by telling us which proteins are modified. Now, in a recent Nature Chemical Biology paper from Linda Hsieh-Wilsons lab at CalTech, they show us a useful new method that reveals what proportion of any particular protein is modified (2%? 80%), and of those that are modified, exactly how many ...

Shafi, R., Iyer, S. P., Ellies, L. G., ODonnell, N., Marek, K. W., Chui, D., Hart, G. W., and Marth, J. D. (2000). The O-GlcNAc transferase gene resides on the X chromosome and is essential for embryonic stem cell viability and mouse ontogeny. Proc Natl Acad Sci U S A. 97, 5735-5739 ...

Background Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X...

GCNT3 antibody for detecting human glucosaminyl (N-acetyl) transferase 3. Validated with up to 12 cell lysates. Try a trial size today.

Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.

The long-term objectives of this proposal are to generate and commercialize a new class of high-specificity, high-affinity proteins called Lectenz?, as research...

How is Composition Evaluation by Lattice Fringe Analysis abbreviated? CELFA stands for Composition Evaluation by Lattice Fringe Analysis. CELFA is defined as Composition Evaluation by Lattice Fringe Analysis very rarely.

i have a 2004 gmc ext. cab 4x4 short box pu gvw is... i have a 2004 gmc ext. Cab 4x4 short box pu gvw is 8100 plusis - Cars & Trucks question

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Trippy, right? Behold the dichotomy that is the Scottish summer.Taken before the rush of the Fringe, when we werent open and could have the beanbags all to ourselves while Barry White croaks over the foyer.

NSString：pathWithComponens:components //根據components中的元素構造有效路徑 NSArray：pathComponents //析構路徑，獲得組成此路徑的各個部分 NSString：lastPathComponent //提取路徑的最後一個組成部分 NSString：pathExtension //從路徑的最後一個組成部分中提取其副檔名 NSString：stringByAppendingPathComponent:path //將path添加到現有路徑的末尾 NSString：stringByAppendingPathExtension:ext //將指定的副檔名添加到路徑的最後一個組成部分 NSString：stringByDeletingLastPathComponent //刪除路徑的最後一個組成部分 NSString：stringByDeletingPathExtension //從檔的最後一部分刪除副檔名 NSString：stringByExpandingTileInPath //將路徑中代字元擴展成用戶主目錄（~）或指定使用者的主目錄（~user） NSString：stringByresolvingSymlinksInPath //嘗試解析路徑中的符號連結 NSString：stringByStandardizingPath ...