CIR Safety Review: The CIR Expert Panel recognizes that the salts of Myristic Acid dissociate to form Myristic Acid and esters of Myristic Acid are hydrolyzed to their corresponding alcohols and Myristic Acid which are then further metabolized. Myristic Acid is a digestible constituent of most vegetable and animal fats and is nontoxic when ingested. Following oral exposure, Butyl Myristate and Ethyl Myristate were not toxic. Dermal exposure indicated that Myristic Acid and Butyl Myristate were not irritating. Myristic Acid, Isopropyl Myristate and Myristyl Myristate were minimally irritating to the eyes. Ethyl Myristate, Glyceryl Myristate and Isopropyl Myristate were not sensitizers. Isopropyl Myristate was negative in genotoxicity tests and was not carcinogenic.. Based on the structural similarities of these compounds, and the knowledge that in the body similar metabolites are formed, the CIR Expert Panel concluded that Myristic Acid, Aluminum Dimyristate, Aluminum Isostearates/Myristate, ...
Plasma membrane targeting of Ras requires CAAX motif modifications together with a second signal from an adjacent polybasic domain or nearby cysteine palmitoylation sites. N-terminal myristoylation is known to restore membrane binding to H-ras C186S (C-186 is changed to S), a mutant protein in which all CAAX processing is abolished. We show here that myristoylated H-ras C186S is a substrate for palmitoyltransferase, despite the absence of C-terminal farnesylation, and that palmitoylation is absolutely required for plasma membrane targeting of myristoylated H-ras. Similarly, the polybasic domain is required for specific plasma membrane targeting of myristoylated K-ras. In contrast, the combination of myristoylation plus farnesylation results in the mislocalization of Ras to numerous intracellular membranes. Ras that is only myristoylated does not bind with a high affinity to any membrane. The specific targeting of Ras to the plasma membrane is therefore critically dependent on signals that are ...
The mechanisms by which proteins are targeted to the membrane of eukaryotic flagella and cilia are largely uncharacterized. We have identified a new family of small myristoylated proteins (SMPs) that are present in Leishmania spp and related trypanosomatid parasites. One of these proteins, termed SMP-1, is targeted to the Leishmania flagellum. SMP-1 is myristoylated and palmitoylated in vivo, and mutation of Gly-2 and Cys-3 residues showed that both fatty acids are required for flagellar localization. SMP-1 is associated with detergent-resistant membranes based on its recovery in the buoyant fraction after Triton X-100 extraction and sucrose density centrifugation and coextraction with the major surface glycolipids in Triton X-114. However, the flagellar localization of SMP-1 was not affected when sterol biosynthesis and the properties of detergent-resistant membranes were perturbed with ketoconazole. Remarkably, treatment of Leishmania with ketoconazole and myriocin (an inhibitor of ...
Myristoylation is a lipidation modification where a myristoyl group, derived from myristic acid, is covalently attached by an amide bond to the alpha-amino group of an N-terminal glycine residue. Myristic acid is a 14-carbon saturated fatty acid (14:0) with the systematic name of n-Tetradecanoic acid. This modification can be added either co-translationally or post-translationally. N-myristoyltransferase (NMT) catalyzes the myristic acid addition reaction in the cytoplasm of cells. This lipidation event is common among many organisms including animals, plants, fungi, protozoans and viruses. Myristoylation allows for weak protein-protein and protein-lipid interactions and plays an essential role in membrane targeting, protein-protein interactions and functions widely in a variety of signal transduction pathways. In 1982, Koiti Titanis lab identified an "N-terminal blocking group" on the catalytic subunit of cyclic AMP-dependent protein kinase from cow as n-Tetradecanoyl. Almost simultaneously in ...
Peng, B.; Voltan, R.; Cristillo, A.D.; Alvord, W.Gregory.; Davis-Warren, A.; Zhou, Q.; Murthy, K.K.; Robert-Guroff, M., 2006: Replicating Ad-recombinants encoding non-myristoylated rather than wild-type HIV Nef elicit enhanced cellular immunity
Buy Myristic Acid ethyl ester (CAS 124-06-1), a 16 carbon saturated fatty acid, from Santa Cruz. Purity: ≥98%, Molecular Formula: C16H32O2, MW: 256.42
Guanylyl cyclase activating protein 1 (GCAP1), a member of the neuronal calcium sensor (NCS) subclass of the calmodulin superfamily, confers Ca(2+)-dependent activation of retinal guanylyl cylcase (RetGC) during phototransduction in vision. Here we analyze the energetics of Ca(2+) and Mg(2+) binding to the individual EF-hands, characterize metal-induced conformational changes, and evaluate structural effects of myristoylation as studied by isothermal titration calorimetry (ITC), differential scanning calorimetry (DSC), and nuclear magnetic resonance (NMR). GCAP1 binds cooperatively to Ca(2+) at EF3 and EF4 (DeltaH(EF3) = -3.5 kcal/mol, and DeltaH(EF4) = -0.9 kcal/mol) with nanomolar affinity (K(EF3) = 80 nM, and K(EF4) = 200 nM), and a third Ca(2+) binds entropically at EF2 (DeltaH(EF2) = 3.1 kcal/mol, and K(EF2) = 0.9 microM). GCAP1 binds functionally to Mg(2+) at EF2 (DeltaH(EF2) = 4.3 kcal/mol, and K(EF2) = 0.7 mM) required for RetGC activation. Ca(2+) and/or Mg(2+) binding to GCAP1 dramatically
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Use the Advanced Search limited to the Perspectives section, to find Perspective articles. Alternatively, the Perspectives and the Perspectives Archive can be accessed from the Literature section of the site.. An example of the proper citation format for a Perspective published prior to January 2008 is shown below:. H. R. de Jonge, B. Hogema, B. C. Tilly, Protein N-myristoylation: Critical role in apoptosis and salt tolerance. Sci. STKE 2000, pe1 (2000).. An example of the proper citation format for a Perspective published after the title change to Science Signaling in January 2008 is shown below:. H. R. de Jonge, B. Hogema, B. C. Tilly, Protein N-myristoylation: Critical role in apoptosis and salt tolerance. Science Signaling 1, pe1 (2008). ...
An additional series of mutational analyses revealed a fine specificity for the lipopeptide recognition by the T cells. The 5-mer Nef peptide conjugated with a shorter (C10) saturated fatty acid (C10-GGAIS) showed reduced T cell stimulation activity compared with C14nef5, and no T cell response was detected for C6-GGAIS (Fig. 1F), further confirming that the peptide modification with a fatty acid of the C14 chain length (myristic acid) was essential for activation of the 2N5.1 cells. The N-terminal amino acid sequence (GGAIS) of the Nef protein matches with a typical N-myristoylation motif, Gly-X-X-X-(Ser/Thr), in which X is any amino acid (3). Whereas the serine-to-threonine substitution (C14-GGAIT) did not affect the antigenic activity, alanine substitution for either the second glycine residue (C14-GAAIS) or the isoleucine residue (C14-GGAAS) located between the conserved flanking amino acid residues totally abrogated the activity (Fig. 1F). Furthermore, addition of an amide linkage to the ...
POL_HV1B1] Gag-Pol polyprotein and Gag polyprotein may regulate their own translation, by the binding genomic RNA in the 5-UTR. At low concentration, Gag-Pol and Gag would promote translation, whereas at high concentration, the polyproteins encapsidate genomic RNA and then shutt off translation (By similarity).[1] Matrix protein p17 has two main functions: in infected cell, it targets Gag and Gag-pol polyproteins to the plasma membrane via a multipartite membrane-binding signal, that includes its myristoylated N-terminus. The second function is to play a role in nuclear localization of the viral genome at the very start of cell infection. Matrix protein is the part of the pre-integration complex. It binds in the cytoplasm the human BAF protein which prevent autointegration of the viral genome, and might be included in virions at the ration of zero to 3 BAF dimer per virion. The myristoylation signal and the NLS thus exert conflicting influences its subcellular localization. The key regulation ...
Low hazard for health. Plant derived, biodegradable and suitable for greywater and septic tanks.. A sugar-based plant derived surfactant used to "solubilize" oils into water and other ingredients which typically are not capable of mixing. It is obtained from renewable raw materials of vegetable origin. Capryly and Myristyl are names to describe fatty acid molecules of different length. 8 carbons long for caprylyl and 14 for myristyl. The glucoside part of its name means that a glucose molecule is attached to these fatty acids.. ...
The Coffey lab seeks a comprehensive understanding of the role of EGF receptor (EGFR) and its ligands in epithelial cell growth, differentiation and neoplasia. In vitro, we study spatial compartmentalization of key components of the EGFR axis in the context of polarized epithelial cells. For example, two critical EGFR ligands, TGF-alpha and amphiregulin, are both delivered exclusively to the basolateral surface, cleaved by TACE/ADAM-17, but then interact with basolateral EGFRs in very different ways. Naked2 and MAGI-3 have been identified recently as two proteins that bind the cytoplasmic tail of TGF-alpha and play important roles in the fidelity (former) and efficiency (latter) of TGF-alphas trafficking to the basolateral surface. Naked2 coats exocytic vesicles that contain TGF-alpha and escorts these vesicles to the basolateral surface where they dock and fuse in a Naked2 myristoylation-dependent manner. Naked2 vesicles have been isolated, 389 proteins have been identified by use of fluorescence
N-myristoylation is a cotranslational modification involved in protein-protein interactions as well as in anchoring polypeptides to phospholipid bilayers; however, its role in targeting proteins to specific subcellular compartments has not been clearly defined. The mammalian myristoylated flavoenzyme NADH-cytochrome b5 reductase is integrated into ER and mitochondrial outer membranes via an anchor containing a stretch of 14 uncharged amino acids downstream to the NH2-terminal myristoylate glycine. Since previous studies suggested that the anchoring function could be adequately carried out by the 14 uncharged residues, we investigated a possible role for myristic acid in reductase targeting. The wild type (wt) and a nonmyristoylatable reductase mutant (gly2--,ala) were stably expressed in MDCK cells, and their localization was investigated by immunofluorescence, immuno-EM, and cell fractionation. By all three techniques, the wt protein localized to ER and mitochondria, while the nonmyristoylated ...
The replication competence of human immunodeficiency virus type 1 genomes containing mutations in the nef open reading frame was evaluated in continuous cell lines. Mutants that contained a deletion in the nef open reading frame, premature termination codons, or missense mutations in the N-terminal myristoylation signal were constructed. The replication of these mutants was tested in three ways. First, plasmid genomes were used to transfect T-lymphoblastoid cells. Second, low-passage posttransfection supernatants were used to infect cells with a relatively low virus input. Third, high-titer virus stocks were used to infect cells with a relatively high virus input. These experiments demonstrated a 100- to 10,000-fold decrement in p24 production by the nef mutants compared with that by the wild-type virus. The greatest difference was obtained after infection with the lowest virus input. The myristoylation signal was critical for this positive effect of nef. To investigate the mechanism of the ...
A 16-residue synthetic peptide corresponding to the N-terminal sequence of p60src was used as the acyl acceptor in an assay for myristoyl-CoA:glycylpeptide N-myristoyltransferase in rat tissues. An additional C-terminal tyrosine amide was added to this peptide to facilitate radioiodination and enhance detectability. Reverse-phase h.p.l.c. enabled the simultaneous detection and quantification of the peptide substrate and its N-myristoylated product. N-Myristoyltransferase activity was highest in the brain with decreasing activities in lung, small intestine, kidney, heart, skeletal muscle and liver. Brain activity was distributed approximately equally between the 100,000 g pellet and supernatant fractions. The soluble enzyme exhibited a Kappm of 20 microM for the src peptide and an optimum between pH 7.0 and 7.5. Maximum N-acylating activity was seen with myristoyl (C14:0)-CoA with lower activities found with the C10:0-CoA and C12:0-CoA homologues. No activity was obtained with palmitoyl ...
প্রয়োজনীয় তেল (Essential oil), নিকট্যান্থিন (nyctanthin), ডি-মানিটল (d-mannitol), ট্যানিন ও গ্লুকোজ (tannin and glucose), ক্যারোটিনয়েড (carotenoid), গ্লুকোসিডেজ (glycosides) যেমন- মনোজেন্টিওবায়োসিডেজ আলফা ক্রোদিটিনের এস্টার (β-monogentiobioside ester of α - crocetin or crocin-3), বিটা-মনোজেনোবায়োসাইড আলফা ক্রোসিটিন ক্যারোটিন এর বিটা-ডি মনোগ্লুকোসাইড এস্টার (β-monogentiobioside -β-D monoglucoside ester of α-crocetin), β-digentiobioside ester of α-crocetin (or crocin-1).. বীজ (Seeds): Arbortristoside A&B, Glycerides of linoleic oleic, lignoceric, stearic, palmitic and myristic acids, nyctanthic acid, 3-4 ...
Genome layout:. VPg+5UTRIRES[L/1AB-1C-1D/2AH-box/NC-2B-2C/3A-3B-3C-3D]3UTR-poly(A). The deduced polyprotein has a length of 2,428 aa. There is a L protein. 1AB protein (with myristoylation signal) remains uncleaved. 2A protein has a H-box/NC sequence motif. ...
The Breast tumor kinase Brk is a prototypical non-myristoylated, non-receptor tyrosine kinase. Brk expression is epithelial-specific and ,in normal tissues, restricted to cells exiting the cell cycle and undergoing terminal differentiation. To determine the biological role of Brk in the gastrointestinal tract, we disrupted mouse brk by homologous recombination. Loss of Brk in the mouse resulted in increased intestinal epithelial cell turnover and the appearance of longer small intestinal villi. Brk deficient mice displayed enhanced accumulation of nuclear (-catenin and upregulation of the (-catenin target gene c-myc in the crypt compartment of small and large intestine. In addition, Brk deficient mice exhibited increased Akt kinase activity. Even though, there was no corresponding difference in base-line apoptosis in untreated wild-type and knockout animals. However, subjected to (-irradiation, Brk deficient animals were significantly impaired in the apoptotic response. Wild-type mice, however, ...
Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. Required for normal embryogenesis.
sized amount, massage in and remove with water.. Package: Tube. INGREDIENT HIGHLIGHTS:. Beeswax, Mild Surfactants. INCIS. Aqua (Water), Glycerin, Potassium Myristate, Sodium Methyl Cocoyl Taurate, Potassium Laurate, Myristic Acid, Cera Alba (Beeswax), Glyceryl Stearate SE, PEG ...
Recombinant protein of human LanC lantibiotic synthetase component C-like 1 (bacterial) (LANCL1), transcript variant 2, 20 ug available for purchase from OriGene - Your Gene Company.
Glycylpeptide N-tetradecanoyltransferase 1 also known as myristoyl-CoA:protein N-myristoyltransferase 1 (NMT-1) is an enzyme that in humans is encoded by the NMT1 gene. GRCh38: Ensembl release 89: ENSG00000136448 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000020936 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Duronio RJ, Reed SI, Gordon JI (May 1992). "Mutations of human myristoyl-CoA:protein N-myristoyltransferase cause temperature-sensitive myristic acid auxotrophy in Saccharomyces cerevisiae". Proc Natl Acad Sci U S A. 89 (9): 4129-33. doi:10.1073/pnas.89.9.4129. PMC 525646 . PMID 1570339. "Entrez Gene: NMT1 N-myristoyltransferase 1". Rajala RV, Datla RS, Moyana TN, et al. (2000). "N-myristoyltransferase". Mol. Cell. Biochem. 204 (1-2): 135-55. doi:10.1023/A:1007012622030. PMID 10718634. Geyer M, Fackler OT, Peterlin BM (2001). "Structure--function relationships in HIV-1 Nef". EMBO Rep. 2 (7): 580-5. doi:10.1093/embo-reports/kve141. PMC 1083955 . ...
Using 5-rapid amplification of cDNA ends, we have identified an extended 5-end of mRNA coding for human myristoyl-CoA:protein N-myristoyltransferase (NMT). PCR using primers based on this new 5-sequence and reverse primers within the currently accepted coding sequence of the enzyme resulted in the identification of a novel splice variant of NMT. In vitro translation of these cDNAs resulted in the production of proteins with apparent molecular masses of 63 kDa and 48 kDa. Immunoprecipitation of NMT from human cell lines and immunoblotting of a range of rat tissues has identified proteins with molecular masses corresponding to those derived from these cDNAs, and provided evidence that their relative abundance differs among tissues. Our results provide evidence that this enzyme exists in different forms resulting from alternative splicing of the mRNA ...
N-Myristoylated ubiquitin ligase Cbl-b inhibitor prevents on glucocorticoid-induced atrophy in mouse skeletal muscleN-Myristoylated ubiquitin ligase Cbl-b inhibitor prevents on glucocorticoid-induced atrophy in mouse skeletal muscle ...
Catalytic domain of the Protein Tyrosine Kinases, Srm and Brk. Protein Tyrosine Kinase (PTK) family; Src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites (Srm) and breast tumor kinase (Brk, also called protein tyrosine kinase 6); catalytic (c) domains. The PTKc family is part of a larger superfamily that includes the catalytic domains of other kinases such as protein serine/threonine kinases, RIO kinases, and phosphoinositide 3-kinase (PI3K). PTKs catalyze the transfer of the gamma-phosphoryl group from ATP to tyrosine (tyr) residues in protein substrates. Srm and Brk are a member of the Src subfamily of proteins, which are cytoplasmic (or non-receptor) tyr kinases. Src kinases in general contain an N-terminal SH4 domain with a myristoylation site, followed by SH3 and SH2 domains, a tyr kinase domain, and a regulatory C-terminal region containing a conserved tyr; they are activated by autophosphorylation at the tyr kinase domain, but are negatively ...
How much of 14:0 tetradecanoic, myristic, common saturated fatty acid is present in Chicken, fryers or broilers, thigh, meat plus skin, cooked, roasted in details, quantity how high or low 14:0 tetradecanoic, myristic, common saturated fatty acid nutrient content it has.
How much of 14:0 tetradecanoic, myristic, common saturated fatty acid is present in Veal, Australian, separable fat, raw natural in details, quantity how high or low 14:0 tetradecanoic, myristic, common saturated fatty acid nutrient content it has.
TY - JOUR. T1 - Myristylation and palmitylation of HSV-1 UL11 are not essential for its function. AU - Baird, Nicholas L.. AU - Starkey, Jason L.. AU - Hughes, David J.. AU - Wills, John W.. PY - 2010/2/5. Y1 - 2010/2/5. N2 - All herpesviruses encode a homolog of the herpes simplex virus type-1 UL11 tegument protein. Deletion of UL11 disrupts virus envelopment, causes capsid accumulation within the cytoplasm, and reduces virus release. UL11 requires acylation with myristate and palmitate for membrane binding, lipid raft trafficking, and accumulation at the site of virus envelopment. Thus, it was predicted that acylation of UL11 would be necessary for efficient virion production, similar to HIV-1 Gag which requires myristylation for virus production. Accordingly, recombinant viruses were created to express UL11 derivatives that are not acylated, are partially acylated, or contain foreign acylation signals. Unexpectedly, the non-acylated UL11 rescued some growth defects of a UL11-null mutant, even ...
BioAssay record AID 144067 submitted by ChEMBL: Tested for Inhibitory concentration against human N-myristoyltransferase (NMT) in radiochemical HPLC end point assay with peptide GNAASARR-NH2 and [3H]myristoyl-CoA radioligand at 1 uM; ND=Not determined.
TY - JOUR. T1 - Differences in eNOS activity because of subcellular localization are dictated by phosphorylation state rather than the local calcium environment. AU - Church, Jarrod E.. AU - Fulton, David. PY - 2006/1/20. Y1 - 2006/1/20. N2 - Nitric oxide (NO) produced in the endothelium via the enzyme endothelial nitric-oxide synthase (eNOS) is an important vasoactive compound. Wild-type (WT) eNOS is localized to the plasma membrane and perinuclear/Golgi region by virtue of N-terminal myristoylation and palmitoylation. Acylation-deficient mutants (G2AeNOS) remain cytosolic and release less NO in response to Ca 2+-elevating agonists; a disparity that we hypothesized was attributed to the greater distance between G2AeNOS and plasma membrane Ca 2+ influx channels. The reduced activity of G2AeNOS versus WT was reversed upon disruption of cellular integrity with detergents or sonication. NO production from both constructs relied almost exclusively on the influx of extracellular Ca2+, and elevating ...
Beneficial effects of myristic ,stearic or oleic acid as part of liposomes on experimental infection & antitumour effect in a murine model ...
Hi all, I want to detect a protein, which has a number of potential motifs potentially useful for detection ! These include sulfation, asn_glycosylation, myristylation and amidation. Is it easy to detect these modification sites and if yes, how do I do this ? Sulfation I have figured out: just add 32S (as H2SO3) to the culture and it (apparently) works. People here have some experience with that already. An additional problem is that I would like to be able to detect by pulse chase the protein amongst total cellular proteins, so f.i. what percentage of proteins is sulfated ? could you reply by e-mail directly as well ? Thanks, clemens suter-crazzolara, anatomy, heidelberg ...
PC(14:0/16:0) is a phosphatidylcholine (PC or GPCho). It is a glycerophospholipid in which a phosphorylcholine moiety occupies a glycerol substitution site. As is the case with diacylglycerols, glycerophosphocholines can have many different combinations of fatty acids of varying lengths and saturation attached at the C-1 and C-2 positions. Fatty acids containing 16, 18 and 20 carbons are the most common. PC(14:0/16:0), in particular, consists of one chain of myristic acid at the C-1 position and one chain of palmitic acid at the C-2 position. The myristic acid moiety is derived from nutmeg and butter, while the palmitic acid moiety is derived from fish oils, milk fats, vegetable oils and animal fats. Phospholipids, are ubiquitous in nature and are key components of the lipid bilayer of cells, as well as being involved in metabolism and signaling.While most phospholipids have a saturated fatty acid on C-1 and an unsaturated fatty acid on C-2 of the glycerol backbone, the fatty acid distribution ...
The reggie protein family consists of two proteins, reggie-1 and -2, also called flotillins, which are highly ubiquitous and evolutionarily conserved. Both reggies have been shown to be associated with membrane rafts and are involved in various cellular processes such as T-cell activation, phagocytosis and insulin signalling. However, the exact molecular function of these proteins remains to be determined. In addition, the mechanism of membrane association of reggie-1, which does not contain any transmembrane domain, is not known. In this study, we have produced a fusion protein of reggie-1 with enhanced green fluorescent protein and generated targeted substitutions for the inactivation of putative palmitoylation and myristoylation sites. We were able to show that reggie-1 is myristoylated and multiply palmitoylated and that lipid modifications are necessary for membrane association of reggie-1. Overexpression of reggie-1 resulted in the induction of numerous filopodia-like protrusions in ...
Browse Fatty Acids - Fractionated, Distilled, Hydrogenated in the McKinley Resources catalog including Caprylic/Capric Acid,Lauric Acid (C-12),Myristic Acid,Oleic Acid,Palmitic Acid,Sodium Cocoyl Isethionate,Stearic Acid
Volume 180ml Ingredients Water, Myristic Acid, Butylene Glycol, Lauric Acid, Disodium Cocoamphodiacetate, Potassium Hydroxide, Glycol Distearate, Glycerin, Sodi
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
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PC(14:0/18:2(9Z,12Z)) is a phosphatidylcholine (PC or GPCho). It is a glycerophospholipid in which a phosphorylcholine moiety occupies a glycerol substitution site. As is the case with diacylglycerols, glycerophosphocholines can have many different combinations of fatty acids of varying lengths and saturation attached at the C-1 and C-2 positions. Fatty acids containing 16, 18 and 20 carbons are the most common. PC(14:0/18:2(9Z,12Z)), in particular, consists of one chain of myristic acid at the C-1 position and one chain of linoleic acid at the C-2 position. The myristic acid moiety is derived from nutmeg and butter, while the linoleic acid moiety is derived from seed oils. Phospholipids, are ubiquitous in nature and are key components of the lipid bilayer of cells, as well as being involved in metabolism and signaling.While most phospholipids have a saturated fatty acid on C-1 and an unsaturated fatty acid on C-2 of the glycerol backbone, the fatty acid distribution at the C-1 and C-2 positions ...
Using video fluorescence microscopy and Golgi proteins tagged with variants of GFP, it has been possible to follow the duplication of the Golgi apparatus in the protozoan parasite T. brucei. A matrix protein and a putative enzyme were chosen for study because in mammals there is evidence that these two types of Golgi protein can behave differently during certain phases of the Golgi life cycle (Seemann et al., 2000, 2002). The GRASP family of matrix proteins has been implicated in cisternal stacking and other signaling events. A single homologue was identified by searching the T. brucei genome that was most similar to mammalian GRASP55 and had the same domain structure: a predicted NH2-terminal myristic acid that aids membrane binding; a highly homologous NH2-terminal region (50% identity) that in mammals binds to the coiled-coil matrix protein GM130 (Barr et al., 1998); and a poorly conserved COOH-terminal region, rich in serines and prolines, which in mammals is thought to mediate mitotic ...
Springfield 4.5" NMT by Danner at Zappos.com. Read Danner Springfield 4.5" NMT product reviews, or select the size, width, and color of your choice.
TY - JOUR. T1 - Creation and expression of myristylated forms of Rous sarcoma virus Gag protein in mammalian cells. AU - Wills, John. AU - Craven, Rebecca. AU - Achacoso, J. A.. PY - 1989. Y1 - 1989. N2 - Rous sarcoma virus (RSV), a member of the avian sarcoma and leukosis family of retroviruses, has long been known to be capable of infecting and transforming mammalian cells; however, such transformed cells do not release virus particles. The RSV gag product (Pr76(gag)) produced in these cells is not released into the culture medium or proteolytically processed to release mature products. Thus, the behavior of Pr76(gag) in mammalian cells is much like that of mammalian retroviral Gag proteins which have been altered so as to block the addition of myristic acid at residue 2 (Gly). Because the RSV gag product does not possess a myristic acid addition site, we hypothesized that the creation of one by oligonucleotide-directed mutagenesis might permit particles to be released from mammalian cells. ...
Jojoba is a desert shrub effective for treating eczema, psoriasis and dry skin.Jojoba oil is more correctly described as liquid wax. Like Macadamia oil, the properties of jojoba oil are akin to that of human sebum or the skins natural oil. As a sunscreen ingredient, jojoba oil not only provides natural moisturizers for the skin but also provides healing and anti-inflammatory elements through its natural substance called myristic acid. Myristic acid is a form of fatty acid naturally occurring in plant oils. This oil has an SPF rating of 4 You May Also Be Interested in:. ...
... , Authors: Atsuhiro Tanabe, Maho Saito. Published in: Atlas Genet Cytogenet Oncol Haematol.
Enterokinase is a protease of the intestinal brush border that specifically cleaves the acidic propeptide from trypsinogen to yield active trypsin. This cleavage initiates a cascade of proteolytic reactions leading to the activation of many pancreatic zymogens. The full-length cDNA sequence for bovine enterokinase and partial cDNA sequence for human enterokinase were determined. The deduced amino acid sequences indicate that active two-chain enterokinase is derived from a single-chain precursor. Membrane association may be mediated by a potential signal-anchor sequence near the amino terminus. The amino terminus of bovine enterokinase also meets the known sequence requirements for protein N-myristoylation. The amino-terminal heavy chain contains domains that are homologous to segments of the low density lipoprotein receptor, complement components C1r and C1s, the macrophage scavenger receptor, and a recently described motif shared by the metalloprotease meprin and the Xenopus A5 neuronal ...
SRMS (Src-related tyrosine kinase lacking C-terminal regulatory tyrosine and N-terminal myristoylation sites) belongs to a family of non-receptor tyrosine kinases, which harbours a Src homology 3 and a Src homology 2, as well as a protein kinase domain. SRMS was first identified in a screen for the genes that regulate the growth and differentiation of neuroepithelial cells. SRMS, however, is an understudied member of this family. The present study was undertaken in order to explore the role of SRMS in signaling downstream of KIT. The receptor tyrosine kinase KIT, also known as the stem cell factor receptor, plays a key role in several developmental processes and have been implicated in many human cancers such as gastrointestinal stromal tumors, acute myeloid leukemia and testicular carcinoma. To understand the role of SRMS in KIT signaling, we generated Ba/F3 cell lines overexpressing KIT and SRMS. We observed that SRMS regulates normal and oncogenic KIT signaling differentially with respect to ...
Here we describe a phosphorylation-based reverse myristoyl switch for mammalian ZNRF2, and show that this E3 ubiquitin ligase and its sister ZNRF1 regulate the sodium/potassium pump (Na+/K+ATPase). N-myristoylation targets ZNRF1 and ZNRF2 to intracellular membranes and enhances their activity. However, when ZNRF2 is phosphorylated in response to agonists including insulin and growth factors, it binds to 14-3-3 and is released into the cytosol. On membranes, ZNRF1 and ZNRF2 interact with the Na+/K+ATPase α1 subunit via their UBZ domains, while their RING domains interact with E2 proteins, predominantly Ubc13 that with Uev1a specifies formation of Lys63-ubiquitin linkages. ZNRF1 and ZNRF2 can ubiquitylate the nucleotide-binding/phosphorylation region of Na+/K+ATPase α1. Ouabain, a Na+/K+ATPase inhibitor and therapeutic cardiac glycoside, decreases ZNRF1 protein levels, while knockdown of ZNRF2 inhibits the ouabain-induced decrease of cell surface and total Na+/K+ATPase α1 levels. Thus, ZNRF1 ...
BioAssay record AID 143943 submitted by ChEMBL: The compound was tested for the affinity against Candida albicans N-myristoyltransferase (NMT).
ADP ribosylation factor 1 (Arf1). Arl1 subfamily. Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Like most members of the Arf family, Arl1 is myristoylated at its N-terminal helix and mutation of the myristoylation site disrupts Golgi targeting. In humans, the Golgi-localized proteins golgin-97 and golgin-245 have been identified as Arl1 effectors. Golgins are large coiled-coil proteins found in the Golgi, and these golgins contain a C-terminal GRIP domain, which is the site of Arl1 binding. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. Arl1 is not required for exocytosis, but appears necessary for trafficking from the endosomes to the Golgi. In Drosophila zygotes, mutation of Arl1 is lethal, and in the host-bloodstream form of Trypanosoma brucei, Arl1 is essential for viability. ...