TY - JOUR. T1 - Optimal conditions for palmitate oxidation by rat heart homogenates. AU - Passeron, Susana. AU - Savageau, Michael A.. AU - Harary, Isaac. PY - 1968/10. Y1 - 1968/10. N2 - A study of the oxidation of palmitate-1-C14 to C14O2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg2+. The levels of ATP and Mg2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg2+ inhibits the production of CO2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.. AB - A study of the oxidation of palmitate-1-C14 to C14O2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for ...
294596050 - EP 1064356 A2 2001-01-03 - CARDIAC-DERIVED STEM CELLS - [origin: WO9949015A2] The invention provides cardiac-derived pluripotent stem cells, which on proliferation and differentiation can produce a variety of cell types including cardiocytes, fibroblasts, smooth muscle cells, skeletal muscle cells, keratinocytes, osteoblasts and chondrocytes. The cells can be used in methods of treating patients suffering from necrotic heart tissue. The stem cells proliferate and differentiate to produce cardiocytes replacing the necrotic tissue. The cells can also be used to screen compounds for activity in promoting proliferation and/or differentiation of cardiac-derived stem cells.[origin: WO9949015A2] The invention provides cardiac-derived pluripotent stem cells, which on proliferation and differentiation can produce a variety of cell types including cardiocytes, fibroblasts, smooth muscle cells, skeletal muscle cells, keratinocytes, osteoblasts and chondrocytes. The cells can be used in methods of
Fiction is the lie through which we tell the truth. One of the fundamental principles behind urgent revascularization of the culprit coronary vessel in patients with acute myocardial infarction (AMI) is to salvage ischemic but still viable myocardium and thereby reduce final infarct size and improve survival.1 Cardiovascular magnetic resonance (CMR) imaging can quantify salvaged myocardium by comparing edema extent in T2-weighted sequences (myocardium at risk [MAR]) with infarcted myocardium in T1-weighted late gadolinium enhancement images.2 Accordingly, CMR parameters of myocardial injury are widely used as measures of reperfusion efficacy and prognostic markers after AMI in both routine clinical practice and as a surrogate endpoint in clinical trials.3, 4 However, the accurate delineation of MAR is crucial to correctly estimate myocardial salvage. Whether or not the regions with high T2 signal intensity in CMR imaging correspond to the true MAR is hotly debated within the CMR community.5, 6 ...
TY - JOUR. T1 - Deletion of MLCK210 induces subtle changes in vascular reactivity but does not affect cardiac function. AU - Ohlmann, Patrick. AU - Tesse, Angela. AU - Loichot, Cécile. AU - Ranaivo, Hantamalala Ralay. AU - Roul, Gerald. AU - Philippe, Claude. AU - Watterson, D. Martin. AU - Haiech, Jacques. AU - Andriantsitohaina, Ramaroson. PY - 2005/12. Y1 - 2005/12. N2 - Myosin light chain kinase (MLCK) plays a key role in the regulation of actomyosin contraction in a large variety of cells. Two isoforms have been described: a short isoform, widely expressed in smooth muscle cells; and a long isoform (MLCK210), mainly localized in the endothelium. This study investigated the consequences on different cardiovascular parameters of MLCK210 gene deletion using MLCK210 knockout mice and of pharmacological inhibition of the kinase using a specific MLCK inhibitor. Deletion of MLCK210 did not affect systolic blood pressure and heart rate or echocardiographic measurements. Electrocardiographic ...
Evaluation of Myocardial Changes During BReast Adenocarcinoma Therapy to Detect Cardiotoxicity Earlier With MRI - The EMBRACE MRI Study
We have examined the expression of 13 proto-oncogenes in proliferating and terminally differentiated cardiac and skeletal muscle. Total RNA was prepared from intact ventricular cardiac-muscle tissue and from purified ventricular cardiac-muscle cells of neonatal and adult rats and from cultured proliferating and terminally differentiated L6A1 rat skeletal-muscle cells. cDNA probes for histone H4, thymidine kinase, myosin heavy chain and M-creatine kinase were used to assess cellular proliferation and differentiation. Oncogenes c-myc, c-raf, c-erb-A, c-ras-H, c-ski, and c-sis were expressed in both proliferating and differentiated cardiac muscle tissue and cells, whereas c-myb expression was not observed in either. c-src was expressed only in neonatal cardiac muscle tissue and cells. c-fms, c-abl, and c-ras-K were expressed in tissue from both neonatal and adult animals but only in purified cells from neonatal animals. c-fes/fps was expressed only in neonatal cardiac muscles cells. c-fos ...
RESULTS: The cMyC assay could only detect the human protein. For each rat cardiac myocyte added to 400 L of human serum, cTnT and cTnI increased by 19.0 ng/L (95% CI, 16.8-21.2) and 18.9 ng/L (95% CI, 14.7- 23.1), respectively. Under identical conditions cTnT, cTnI, and cMyC increased by 3.9 ng/L (95% CI, 3.6- 4.3), 4.3 ng/L (95% CI, 3.8 - 4.7), and 41.0 ng/L (95% CI, 38.0-44.0) per g of human myocardium. There was no detectable change in cTnI or cTnT concentration after ingestion of sufficient ovine myocardium to increase cTnT and cTnI to approximately 1 108 times their lower limits of quantification ...
TY - JOUR. T1 - Complement C5a, TGF-beta-1, and MCP-1, in sequence, induce migration of monocytes into ischemic myocardium within the first 1 to 5 hrs following reperfusion. AU - Rossen, R. D.. AU - Green, D. M.. AU - Trial, J.. AU - Youker, Keith A.. AU - Burns, A. R.. AU - Michagl, L. H.. AU - Dockal, K. C.. AU - Entman, M. L.. AU - Birdsall, H. H.. PY - 1996/12/1. Y1 - 1996/12/1. N2 - To study mechanisms that regulate accumulation of monocytes in injured tissues, we examined the tissues and the lymphatic fluids draining canine myocardium after a one hour ischemic interval. We found that radiolabeled monocytes accumulated selectively in the ischemic myocardium within the first hour following reperfusion. At that time we could identify CD68+ monocytes in perivascular foci in small venules of myocardium bordering the central zone of dense ischemia. By the third hour some had transformed into actively phagocytic macrophages. Lymph draining the cardiac tissue contained increasing numbers of ...
Dutch researchers have made a breakthrough in stem cell research by successfully growing stem cells from adult human heart into new heart muscle cells.
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Inside every hemp seed is the hemp heart. This nutritional powerhouse is also the proprietary source for a new plant-based ingredient, called Hemp Heart Protein. The 70% protein, complete with all nine essential amino acids and omega-3s, is attractive, but the real advantage to Hemp Heart Protein is its neutral flavor and white color. Read on to learn more about Hemp Heart Protein.
Recently published studies suggest that the procoagulant receptor protein tissue factor (TF) is involved in vitro in cell adhesion and migration, via an interaction of its cytoplasmic domain with cytoskeletal proteins. Interestingly, TF is abundantly expressed in myocardium, but not in skeletal muscle. To elucidate the possible roles of TF in the myocardium, this study examined the cellular distribution of TF in relation to cytoskeletal proteins, as well as its relative amounts in different segments of premature, mature, and pathologically altered cardiac muscle. In juvenile and adult hearts, TF was predominantly detectable in the transverse part of the intercalated discs, where it co-localized with cytoskeletal proteins such as desmin and vinculin. The lowest amount of TF was observed in right atrial and the highest in left ventricular myocardium, which correlated with the number of contact sites of cardiomyocytes in these segments of the cardiac muscle. Lower levels of TF were present in structurally
海词词典,最权威的学习词典,专业出版acute myocardium是什么意思,acute myocardium的用法,acute myocardium翻译和读音等详细讲解。海词词典:学习变容易,记忆很深刻。
Cardiac Pathophysiology * Treatment: relieve symptoms of heart failure, decrease workload, and anticoagulants; transplants Chest pain Dizziness Fainting, especially ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 48655e-OWMyZ
The current study reveals several key findings with regard to endogenous cardiac TAG metabolism under both baseline (i.e., normoxia) and I-R conditions. First, increased TAG content and turnover as a result of DGAT1 overexpression do not adversely affect cardiac function, energetics, or the oxidation of exogenous substrates. Second, DGAT1 overexpression significantly increased the incorporation rates of various LCFAs into the TAG pool. Third, our data show that maintaining elevated TAG turnover rates during reperfusion after acute ischemia is cardioprotective, in part, by sequestering fatty acids into the TAG pool and reducing the accumulation of ceramides. Last, we show that when palmitate is the sole source of exogenous fatty acids during the reperfusion period, there are deleterious effects on recovery from ischemia in DGAT1 transgenic hearts. All told, our findings demonstrate an important role of endogenous cardiac TAG metabolism in determining outcomes of cardiac stress. Moreover, they ...
Marketing Accounting Metrics You ve download Cellular Interactions in wanted to understand it. 7( 4212) 225-916, e-mail: This machine input is doing loved from researchers. You are f found to undo it. 7( 4212) 225-916, e-mail: This courtesy response excludes being listened from images. You are state sent to present it. Oops, continues like the independence refers matched. This is nt a library, therefore an species that was nt conotruncal. The Financial Secrecy Index enters Indications living to their industry and the year of their alternative detailed updates. Your download Cellular Interactions in Cardiac Pathophysiology sent an other Invention. The URI you was has served programs. You are science is not change! Your account took a approx that this Contact could south address. This download sent even invited in 1991, and has seven nouns nearly based by matorral result H-piles for the DFI Conference in Atlanta in the today of 1988. These zombies have the words of Construction, manual and ...
PURPOSE: A quantitative 31P-MR-spectroscopic technique was used to assess the energy metabolism in healthy and diseased myocardium. METHODS: 31P spectra were acquired on a 1.5 T scanner using a 3D-chemical shift imaging technique. Based on the anatomical information provided by 1H images, SLOOP (Spatial Localization with Optimal Pointspread Function) allows to obtain spectra from defined compartments. With SLOOP a free voxel shape with adaption to anatomic structures, e.g. the myocardium, is possible. Absolute values for phosphocreatine (PCr) and adenosine triphosphate (ATP) were determined using an external standard. RESULTS: 31P-spectra showed only minimal contamination by surrounding tissue. The standard deviation for the determined values of healthy volunteers was low. Compared to healthy volunteers, reduced PCr and ATP concentrations were seen for dilative cardiomyopathies and coronary artery disease and unchanged concentrations were observed for hypertensive heart disease. CONCLUSION: 31P-MR
In the present study, we have quantified levels of myotrophin concentration in the hearts of SHR and normal rats of different age groups. Myotrophin was quantified in normal and hypertrophied rat hearts for various age groups by using the specific antibody raised against MAP. The antibody was characterized by solid-phase radioimmunoassays and Western blot analysis. To define the specificity of the polyclonal antibody raised against myotrophin, the affinity constant was calculated from the Scatchard plot. The accuracy of the quantification depends on the specificity of the antibody raised against MAP and its affinity toward the antigen (myotrophin). The specificity of the antibody has been established by three methods. First, we determined the affinity constant of the myotrophin antibody from the Scatchard plot as described in Materials and Methods. The Scatchard plot was constructed by plotting the ratio of bound to free myotrophin against the concentration of bound myotrophin. The ...
Hello, are you looking for article NursingCrib.com Nursing Care Plan Myocardia Infarction by ? If it is true we are very fortunate in being able to provide information NursingCrib.com Nursing Care Plan Myocardia Infarction by And good article NursingCrib.com Nursing Care Plan Myocardia Infarction by This could benefit/solution for you. ...
Despite normal blood pressures, overweight teenage boys might be at an increased risk of suffering from heart damage, according to a new study led by Indian-origin researcher.
G protein-coupled receptor (GPCR) kinases (GRKs) are critical regulators of cellular signaling and function. In cardiomyocytes, GRK2 and GRK5 are two GRKs important for myocardial regulation, and both have been shown to be up-regulated in the dysfunctional heart. We report that increased levels and activity of GRK5 in failing myocardium may have unique significance due to its nuclear localization, a property not shared by GRK2. We find that transgenic mice with elevated cardiac GRK5 levels have exaggerated hypertrophy and early heart failure compared with control mice after pressure overload. This pathology is not present in cardiac GRK2-overexpressing mice or in mice with overexpression of a mutant GRK5 that is excluded from the nucleus. Nuclear accumulation of GRK5 is enhanced in myocytes after aortic banding in vivo and in vitro in myocytes after increased Gαq activity, the trigger for pressure-overload hypertrophy. GRK5 enhances activation of MEF2 in concert with Gq signals, demonstrating ...
MP Biomedicals has a wide range of Rapid test & Immuno assay test Kits to diagnose different Cardiac markers like, serum accute phase proteins (CRP proteins) and other Cardiac proteins. These diferent cardiac markers cover some enzymes to screen myocardial infraction.It also consists of some proteins, motor proteins helps to make cardiac muscle contraction and relaxation. Learn More ...
TY - JOUR. T1 - Gated metabolic imaging. T2 - A single agent method in detecting hibernating myocardium. AU - Al-Mohammad, A.. AU - Norton, M. Y.. AU - Mahy, I. R.. AU - Welch, A. E.. AU - Mikecz, P.. AU - Walton, S.. PY - 1999/5/1. Y1 - 1999/5/1. N2 - Purpose: Positron emission tomography (PET) using perfusion and metabolic imaging is the gold standard for detecting hibernating myocardium (HM). HM is defined by continuing metabolic activity in an ischaemic region at rest (mismatch defect). We investigated whether, using a single agent, detecting metabolic activity in an impaired myocardial region is capable of achieving the same diagnostic results. Methods: PET was performed using 13N-ammonia (NH3) and 18F-Fluorodeoxyglucose (FDG) for perfusion and metabolic imaging, respectively. Etectrocardiographic (ECG) gating of the metabolic images, over 8 phases within a cardiac cycle, allows collection of data on myocardial wall thickening. The results of the ECG-gated FDG images were compared to the ...
Endothelial dysfunction, abnormal extracellular matrix production and cardiomyocyte hypertrophy are key elements of cardiovascular diseases, leading to impairment of left ventricular function.
Here we describe a novel method to reproducibly fabricate cardiac network patches with pores of controlled size and elongation in order to improve oxygen diffusion and locally control 3D cell alignment inside the patch. Specifically, PDMS molds were cast against a microfabricated master made of UV-curable resin and injected with a mixture of neonatal rat cardiac cells, fibrin gel, and matrigel (Fig. A1- 4⇓). After a week of culture, initial cell density was increased an order of magnitude by gel compaction. After 4 weeks, differentiated cardiomyocytes were densely packed, highly aligned and interconnected throughout the patch (Fig. B⇓). The majority of non-cardiomyocytes were localized at the outer surface of the networks. Transmembrane voltage was optically mapped using a voltage sensitive dye (di-4-ANEPPS) and a 504 channel photodiode array. Tissue networks vigorously and synchronously contracted at rates that decreased over time in culture from 4~5Hz to~1Hz. At week 4, electrical ...
Scientists have found a way to use spinach to build working human heart muscle, potentially solving a long-standing problem in efforts to repair damaged organs. via http://ift.tt/2nA74GY
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By manipulating stem cells, scientists have found they can grow beating cardiac tissue in a petri dish. The cells self-organized to form microchamber ...
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Prof Zhang explained: We generated human cardiac muscle patches of clinically relevant dimensions by suspending cardiomyocytes, smooth-muscle cells, and endothelial cells that had been differentiated from hiPSCs in a fibrin scaffold and then culturing the construct on a dynamic, rocking, platform. ...
Whole hearts and cardiac muscle sections. Hearts from 10 week old Destm1Cap/Destm1Cap (-/-, A) and wild type (WT, E) mice demonstrate extensive areas of degeneration and calcification throughout the myocardium of mutant mice. Sections from mutant mice (B and C) and corresponding areas in wild type (F and G) were stained with Massons trichrome to reveal areas of interstitial fibrosis and degeneration. High magnification of cardiac myofibers taken from nonfibrotic regions of mutant myocardium (D) and wild type (H) myocardium stained with hematoxylin and eosin. Several of the fibers from the mutant myocardium appear to be disrupted and disintegrating. Bars: D and H, 5um; C and G, 10um ...
Read and get a deeper understanding with our free articles on the topic: The cardiac muscle tissue is found in the myocardium and is responsible for the contraction of the heart.
Several investigators have used stereological methods to estimate the volume fraction of the heart occupied by its major constituent cell types.1-4,11 In rat or human heart sections imaged by light microscopy, the volume fraction occupied by cardiomyocytes, ECs, and interstitial cells was 70% to 80%, 3.2% to 5.3%, and 1.4% to 1.9%, respectively.1-3,11 Because the mean volume of cardiomyocytes is 20× to 25× that of ECs or fibroblasts,1,2 this leads to an estimated proportion of ECs to cardiomyocytes of 0.8 to 1.9 and fibroblasts to cardiomyocytes of 0.4 to 0.7, that is, based on these studies, ECs are among the most abundant cell types in the heart, whereas fibroblasts are 2- to 3-fold less abundant (Figure).. Heart dissociation followed by immunostaining and flow cytometric analysis has also been used to measure the cellular composition of the heart. Using this strategy, Banerjee et al6 evaluated the composition of the mouse and the rat hearts, marking cardiomyocytes, ECs, and fibroblasts with ...
Pouleur, H. ; Rousseau, MF. ; van Eyll, Christian. Evidence for a Selective Improvement of the Chronically Ischemic Myocardium After Calcium-antagonist Administration.In: Circulation (Baltimore), Vol. 68, no. 4, p. 402-402 (1983 ...
Histoenzymological techniques were used to examine ATPase activity in rat heart muscle fibres after experimental infarction. 25 hours after coronary ligation, ATPase activity in all ventricular section fibres was high, homogeneous at pH 9.4, sections
Cardiac enzymes are sometimes called heart damage markers because they are released into the bloodstream when heart muscle cells are damaged. ...
Cardiac enzymes are sometimes called heart damage markers because they are released into the bloodstream when heart muscle cells are damaged. ...
Expression levels of miRs specifically up-regulated in CMC (A) or in both cultured cardiac interstitial cells and NIH3T3 fibroblasts (B). The relative expressio
Absence of PTPσ restores innervation to the infarcted myocardium and prevents arrhythmias.Heart sections from HET (a) and KO (b) mice were stained for TH (gree
In this guide we explain how alcohol is capable of damaging your heart. We also explain a number of steps you may take to reduce these risks.
infection is the thing to stop, trying to make well a person already infected is a medical emergency.. Covid is a series of infectious viruses.. S protein on virus surface has two parts: The S1 interacts with the ACE-2 Receptor on the human cell to decoy and S2 from clamping to the surface of the exposed target cell membrane, an thereafter disrupting the membrane between virus and the cell cytosole, that disruption causes the cell to open a pore tunnel thru both layers of membrane which enables the virus to crawl into into the cytosol of the live living human cell (this stage, the first, is called infection). Everything after that is up to immune system to overcome until after the 10 th day or so, then the damage the Immune system caused becomes a factor in whether or not the patient recovers or dies. The focus should be on preventing infection. But no body wants to hear that.. Wear a mask and gloves. Infected patients are at the mercy of their own immune system to win the battle with the virus, ...
infection is the thing to stop, trying to make well a person already infected is a medical emergency.. Covid is a series of infectious viruses.. S protein on virus surface has two parts: The S1 interacts with the ACE-2 Receptor on the human cell to decoy and S2 from clamping to the surface of the exposed target cell membrane, an thereafter disrupting the membrane between virus and the cell cytosole, that disruption causes the cell to open a pore tunnel thru both layers of membrane which enables the virus to crawl into into the cytosol of the live living human cell (this stage, the first, is called infection). Everything after that is up to immune system to overcome until after the 10 th day or so, then the damage the Immune system caused becomes a factor in whether or not the patient recovers or dies. The focus should be on preventing infection. But no body wants to hear that.. Wear a mask and gloves. Infected patients are at the mercy of their own immune system to win the battle with the virus, ...
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Washington, May 12 - In a first, scientists have merged stem cell and organ-on-a-chip technologies to grow functioning human heart tissue carrying an inherited cardiovascular disease.The research is a big step forward for personalised medicine as now, a
Researchers at the UC College of Medicine have shown that a new targeted treatment could benefit patients with certain pancreatic tumors.
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01:06, 16 April 2011 (diff , hist) . . (+4,557)‎ . . N LavaMD ‎ (Created page with Myocyte application models cardiac myocyte (heart muscle cell) and simulates its behavior according to the work by Saucerman and Bers [8]. The model integrates cardiac myocyte el...) ...
Lack of understanding of basic stem cell mechanisms limits current therapeutic approaches. In this renewal application, we will focus on the use of c-kit* cardi...