Danggui (Radix Angelicae Sinensis) is an herb often used in Traditional Chinese medicine. It is used to promote blood flow and has been used in the treatment of myocardial ischemia-reperfusion injury in animal models. Angiotensin II (Ang II) has been shown to play important roles in mediating cardiovascular diseases, and may cause cardiac hypertrophy and apoptosis. This study aimed to investigate whether Danggui has protective effects on Ang II-induced apoptosis in H9c2 cardiomyoblast cells and study the mechanisms involved. We evaluated the effect of Danggui on Ang II-induced apoptosis in an in vitro model. H9c2 cardiomyoblast cells were cultured in serum-free medium for 4 hr, then treated with Danggui (50, 100 μg/ml) 1 hr pre- or post-Ang II treatment. After a further 23 hr of culture, cells were harvested for analyses with assays for apoptosis markers and cell signaling pathways. Our results showed that Ang II induced upregulation of pro-apoptotic Bad, instability of the mitochondria membrane

During normal lifespan, the mammalian heart undergoes limited renewal of cardiomyocytes. While the exact mechanism for this renewal remains unclear, two possibilities have been proposed: differentiated myocyte replication and progenitor/immature cell differentiation. This study aimed to characterize a population of cardiomyocyte precursors in the neonatal heart and to determine their ... read more requirement for cardiac development. By tracking the expression of an embryonic Nkx2.5 cardiac enhancer, we identified cardiomyoblasts capable of differentiation into striated cardiomyocytes in vitro. Genome-wide expression profile of neonatal Nkx2.5+ cardiomyoblasts showed the absence of sarcomeric gene and the presence of cardiac transcription factors. To determine the lineage contribution of the Nkx2.5+ cardiomyoblasts, we generated a doxycycline suppressible Cre transgenic mouse under the regulation of the Nkx2.5 enhancer and showed that neonatal Nkx2.5+ cardiomyoblasts mature into cardiomyocytes ...

Background: Doxorubicin (DOX), a widely used anticancer drug, has been associated with cardiotoxicity. Recently, DOX-induced cardiotoxicity has been attributed to topoisomerase II (TOPII)-β expression and activity. In our study, we investigated the effect of inhibiting TOPII in attenuating the DOX induced cardiotoxicity. Method: H9c2 cardiomyoblasts were treated with 1 or 2 µM DOX (+/-) 1 µM ETO. Cardiotoxicity was assessed by examining cell viability using the MTT assay, hypertrophy of crystal violet stained cardiomyoblasts and ROS production. Results: DOX induced a dose dependent increase in cardiotoxicity as indicated by the significant reduction in cell viability (71.77 ± 9.25% 2 µM DOX vs. 100% control, P,0.05), ROS production and hypertrophy. Stimulation of H9c2 cardiomyoblasts with both 2 µM DOX and 1µM ETO did not show a significant difference in cell viability, ROS production or hypertrophy. Conclusion: DOX induced cardiotoxicity in H9c2 cardiomyoblasts was not exacerbated in the ...

Keywords: Body organ preservation alternative, Cardiac myoblasts, Mitochondria, Bioenergetics, Somah, Celsior, Perfadex Background The silver standard for sufferers experiencing end-stage heart failing remains center transplantation. With the real variety of sufferers needing transplants developing as well as the obtainable organs PI-103 staying continuous, theres a dependence on improved donor body organ and graft preservation solutions to extend the life span of donor organs [1]. Conserving body organ viability beyond an organism could be achieved by frosty or warm storage space, with or without perfusion. Presently, the standard strategy in center preservation is frosty static storage space, supplemented through preservation solutions [2] often. These approaches have got allowed for an ex girlfriend or boyfriend vivo storage space time around 4C6 h before vital tissue damage takes place [3]. Preservation of organs for much longer than 4C6 h leads to increased ROS amounts, ATP depletion, ...

Background: Mammalian Target of Rapamycin (mTOR) is a central sensor of nutrient availability and cellular stress and is implicated in carcinogenesis and acceleration of aging. Since iron is a required nutritional element, we assessed the role of mTOR in regulation of cellular iron homeostasis.. Methods and Results: To determine whether mTOR regulates iron homeostasis, we analyzed the expression of genes necessary for cellular iron uptake, storage, mitochondrial handling and export with mTOR modulation. Pharmacologic inhibition of mTOR with rapamycin decreased expression of several iron-regulatory genes in mouse embryonic fibroblasts (MEFs) and led to accumulation of heme and non-heme iron in H9C2 cardiac myoblasts. Constitutive activation of mTOR by genetic ablation of its inhibitor in TSC2−/− MEFs enhanced the expression of the above genes and decreased cellular iron content; both findings were reversible with rapamycin treatment. To study the mechanism of iron regulation by mTOR, we ...

Intracellular accumulation of fatty acid metabolites in non-adipose tissues has been proposed to play an important role in the pathogenesis of diabetes mellitus. Moreover, lipotoxicity may contribute to cell death and cardiac dysfunction. Evidence is emerging that saturated fatty acids induce cell death through apoptosis and this effect is specific for palmitate and stearate. On the other hand, n-3 polyunsaturated fatty acids (PUFAs) have been implicated in the protection against cardiovascular diseases, cardiac ischemic damage and myocardial dysfunction. In the present study we show- that supplementation with the n-3 PUFA- eicosapentaenoic acid (EPA)- to culture medium of H9c2 rat cardiomyoblasts protects cells against palmitate-induced apoptosis. Experiments were performed by using H9c2 rat ventricular cardiomyoblasts. Apoptosis was detected by trypan blue exclusion, MTT or caspase activity assays. Gene expression was investigated by Real Time PCR. Signal transduction pathways were ...

Quercetin Reduces Inflammatory Responses in LPS-Stimulated Cardiomyoblasts. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.

The final morphogenesis of the cardiac outflow tract (OFT) depends on coordinated cell-cell interactions among the heart tip, migrating heart-anchorin...

Predicted to have RNA polymerase II distal enhancer sequence-specific DNA binding activity. Involved in brain development; cardioblast differentiation; and cell fate specification. Predicted to ... Predicted to have RNA polymerase II distal enhancer sequence-specific DNA binding activity. Involved in brain development; cardioblast differentiation; and cell fate specification. Predicted to localize to the nucleus. Is expressed in several structures, including mesoderm; nervous system; otic vesicle; pharyngeal arch; and pronephric duct. Orthologous to human HOXB5 (homeobox B5). ...

Following the safety verification of the Phase I study (TICAP trial) that has been implemented and completed, the efficacy will be verified in this Phase II clinical study. The rationale of this study is based on the animal studies that transplanted cardiac progenitor cells may regenerate damaged myocardium by giving rise to cardiac muscle and vascular cell types. Preclinical and phase I studies indicate the possibilities of cardiac function improvements and reduced myocardial fibrosis by progenitor cell infusion.. The efficacy of cell transplantation is assessed as the primary endpoint of the Phase II clinical study. The improvements of cardiac ejection fraction assessed by echocardiography, ventriculography, and cardiac MRI, which are conducted before and after treatment, are assessed as the evaluation items. The absolute values of the improvement of cardiac ejection fraction obtained by the above three imaging modalities before and after treatment are compared between two groups of the ...

Catecholamines enhance cardiac function by activating effector molecules targeting Ca2+ handling proteins in myocytes, but the impact of these neurohumoral factors on the pool of resident primitive cells is poorly defined. The aim of this study was to establish whether catecholaminergic stimulation of human cardiac progenitor cells (hCPCs) promotes intracellular Ca2+ oscillations, critical events for cell cycle progression. For this purpose, c-kit-positive hCPCs were loaded with the Ca2+ indicator Fluo-4 and monitored by two-photon microscopy. After documenting that intracellular Ca2+ elevations in hCPCs are mediated by Ca2+ translocation from the endoplasmic reticulum to the cytoplasm via IP3 receptor channels, the effects of adrenergic receptor (AR) stimulation were tested. In Tyrode solution, Ca2+ spikes were observed in 12% of the cell population over a period of 33 minutes, and exposure to norepinephrine, agonist of α- and β-AR, increased the fraction of active cells to 26%. To dissect ...

miR-133a and miR-1 are known as muscle-specific microRNAs that are involved in cardiac development and pathophysiology. We have shown that both miR-1 and miR-133a are early and progressively upregulated during in vitro cardiac differentiation of adu

Discovery of cardiac progenitor cells (CPCs) in the adult tieart hias led to tieightened expectations for novel treatments of cardiac disease. However, adoptive...

Danshen-Gegen decoction exerts proliferative effect on rat cardiac myoblasts H9c2 via MAPK and insulin pathways.. PubMed. Fong, Chi Chun; Wei, Fan; Chen, Yao; Yu, Wai Kin; Koon, Chi Man; Leung, Ping Chung; Fung, Kwok Pui; Lau, Clara Bik San; Yang, Mengsu. 2011-10-31. Danshen (root of Salvia miltiorrhiza) and Gegen (roots of Pueraria lobata) are traditional Chinese medicines that have been used in combination for cardiovascular disease treatment. The present study was performed to investigate the effect of Danshen-Gegen decoction on rat myocardium cell line H9c2 and the possible molecular mechanisms. Rat heart myocardium H9c2 cells were treated with or without Danshen-Gegen decoction (DG) ranging from 10 to 1000μg/ml for 24h. Cell viability was measured by Alarma blue assay and cell proliferation assay was performed by BrdU Cell Proliferation ELISA kit. The activation of mitogen-activated protein kinase and insulin pathways was analyzed by Luminex technology and the growth factors and cytokine ...

TY - JOUR. T1 - IGF-II/mannose-6-phosphate receptor signaling induced cell hypertrophy and atrial natriuretic peptide/BNP expression via Gq interaction and protein kinase Cα-/CaMKII activation in H9c2 cardiomyoblast cells. AU - Chu, Chun Hsien. AU - Tzang, Bor Show. AU - Chen, Li Mien. AU - Kuo, Chia Hua. AU - Cheng, Yi Chang. AU - Chen, Ling Yun. AU - Tsai, Fuu Jen. AU - Tsai, Chang Hai. AU - Kuo, Wei Wen. AU - Huang, Chih Yang. N1 - Copyright: Copyright 2009 Elsevier B.V., All rights reserved.. PY - 2008/5. Y1 - 2008/5. N2 - The role played by IGF-II in signal transduction through the IGF-II/ mannose-6-phosphate receptor (IGF2R) in heart tissue has been poorly understood. In our previous studies, we detected an increased expression of IGF-II and IGF2R in cardiomyocytes that had undergone pathological hypertrophy. We hypothesized that after binding with IGF-II, IGF2R may trigger intracellular signaling cascades involved in the progression of pathologically cardiac hypertrophy. In this study, ...

Endocardial and myocardial progenitors originate in distinct regions of the anterior lateral plate mesoderm and migrate to the midline where they coalesce to form the cardiac tube. Endocardial progenitors acquire a molecular identity distinct from other vascular endothelial cells and initiate expression of specific genes such as nfatc1. Yet the molecular pathways and tissue interactions involved in establishing endocardial identity are poorly understood. The endocardium develops in tight association with cardiomyocytes. To test for a potential role of the myocardium in endocardial morphogenesis, we used two different zebrafish models deficient in cardiomyocytes: the hand2 mutant and a myocardial-specific genetic ablation method. We show that in hand2 mutants endocardial progenitors migrate to the midline but fail to assemble into a cardiac cone and do not express markers of differentiated endocardium. Endocardial differentiation defects were rescued by myocardial but not endocardial-specific ...

Rationale: Myocardial infarction (MI) is a leading cause of death in developed nations, and there remains a need for cardiac therapeutic systems that mitigate tissue damage and. Cardiac progenitor cells (CPCs) and other stem cell types are attractive candidates for treatment of MI; however, the benefit of these cells may be due to paracrine effects. Objective: We tested the hypothesis that CPCs secrete pro-regenerative exosomes in response to hypoxic conditions. Methods and Results: The angiogenic and anti-fibrotic potential of secreted exosomes on cardiac endothelial cells and cardiac fibroblasts were assessed. We found that CPC exosomes secreted in response to hypoxia enhanced tube formation of endothelial cells and decreased pro-fibrotic gene expression in TGF-β stimulated fibroblasts, indicating that these exosomes possess therapeutic potential. Microarray analysis of exosomes secreted by hypoxic CPCs identified eleven miRNAs that were upregulated compared to exosomes secreted by CPCs grown ...

Capillary electrophoretic mobility shift assay for binding of DNA with NFAT3, a transcription factor from H9c2 cardiac myoblast cells ...

Self-assembling heart-derived stem cell clusters named cardiospheres (CSps) improve function and attenuate remodeling in rodent models of acute myocardial infarction. The effects of CSps in chronically remodeled myocardium post-MI, and the underlying mechanisms, remain unknown. One month after permanent coronary ligation, rats were randomly assigned to injection of vehicle (controls) or CSps in the peri-infarct area. One month post-injection, CSps increased left ventricular function, reduced scar mass and collagen density, and enhanced vascularity within the infarct zone compared to controls. Immunoblots revealed Tgfbeta-1/smad cascade downregulation and an increase in soluble endoglin post-CSp injection. Six months post-transplantation, left ventricular function further improved and cardiomyocyte hypertrophy was attenuated in the CSp-treated group. In vitro, co-culture of CSps with fibroblasts recapitulated the suppression of the Tgf-beta1/smad pathway changes, responses which were blunted by ...

PURPOSE: To assess the uptake, accumulation, temporal stability, and spatial localization of isoflurane (ISO) in the C57BL/6 mouse, and to identify its potential interference with the detection of labeled cardiac progenitor cells using 19 F MRI/MR spectroscopy (MRS). MATERIALS AND METHODS: Objectives are demonstrated using (a) in vitro ISO tests, (b) in vivo temporal accumulation/spatial localization C57BL/6 studies (n = 3), and (c) through injections of perfluoro-crown-ether (PFCE) labeled cardiac progenitor cells into femoral muscle areas of the murine hindlimb post-mortem (n = 1) using 1 H/19 F MRI/MRS at 9.4 Tesla. Data were acquired using double-gated spoiled gradient echo images and pulse-acquire spectra. For the in vivo study, the temporal stability of ISO resonances was quantified using coefficient of variability (CV) (5 min) estimates. RESULTS: Two ISO resonances were observed in vivo that correspond to the -CF3 and -OCHF2 moieties. CV values ranged between 3.2 and 6.4% (-CF3 ) and 6.4 and 11.2

The four-chambered mammalian heart develops from two fields of cardiac progenitor cells (CPCs) distinguished by their spatiotemporal patterns of differentiation and contributions towards the definitive heart [1-3]. lack of function research in zebrafish a lesser vertebrate with an individual ventricle that transcripts tag a field of CPCs with determining characteristics from the anterior SHF in mammals. Particularly absence the same cardiac constructions produced from cells because of jeopardized progenitor proliferation. Additionally small-molecule inhibition of TGFβ signaling phenocopies the hybridization we found that zebrafish transcripts are indicated in cells in the outflow pole from the linear center pipe (Fig. 1a b and Supplementary Fig. 1 and 2). Two times marker analyses verified that a most cells are nonoverlapping with differentiated myocardium (Fig. 1c-e and Supplementary Fig. 2). Additionally cells are neither endothelial nor LY315920 produced from the neural crest because ...

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Purpose: NecroX-5 is an indole-derived small molecule which showed anti-oxidative and anti-nitrosative effects in H9C2 cardiomyoblast and protective effect against in vivo ischemia-reperfusion injury. In this study, we examined the effect of NecroX-5 in an animal model for retinal degeneration (RD), which is one of the leading causes of photoreceptor cell death.. Methods: Retinal degeneration was induced in Sprague-Dawley rats by an intraperitoneal injection of N-methyl-N-nitrosourea (MNU) (75 mg/Kg), a DNA methylating agent, which causes selective retinal pigment epithelium damage with secondary photoreceptor cell death. NecroX-5 (50 μM) was injected intravitreally at 3 h after MNU injection. Electroretinogram (ERG) recordings and morphological analyses were performed at 1 week after MNU injection.. Results: Marked loss of photoreceptors in the outer nuclear layer (ONL) was observed in untreated RD rats at 1 week after MNU injection, while the ONL in the MNU-induced RD rats injected with ...

The potential of marine benthic cyanobacteria as a source of anticancer drug candidates was assessed in a screen for induction of cell death (apoptosis) in acute myeloid leukemia (AML) cells. Of the 41 marine cyanobacterial strains screened, more than half contained cell death-inducing activity. Several strains contained activity against AML cells, but not against non-malignant cells like hepatocytes and cardiomyoblasts. The apoptotic cell death induced by the various strains could be distinguished by the role of caspase activation and sensitivity to the recently detected chemotherapy-resistance-associated prosurvival protein LEDGF/p75. One strain (M44) was particularly promising since its activity counteracted the protective effect of LEDGF/p75 overexpressed in AML cells, acted synergistically with the anthracycline anticancer drug daunorubicin in AML cells, and protected cardiomyoblasts against the toxic effect of anthracyclines. We conclude that culturable benthic marine cyanobacteria from temperate

Vertebrate hearts develop from the fusion of bilaterally positioned cardiac precursors, followed by the growth of the primitive heart tube. In the zebrafish, complex morphogenic events transforming the embryonic heart from sheets of cardiac precursors into a three-dimensional tubular structure have been previously described (Stainier and Fishman, 1992; Yelon, 2001; Yelon et al., 1999). The proper growth of the primitive heart tube is an important factor for subsequent patterning of cardiac chambers, as demonstrated by studies on the zebrafish heart and soul mutation (Peterson et al., 2001; Yelon et al., 1999). However, detailed molecular and cellular mechanisms guiding primitive heart tube extension are still largely unknown.. Na,K-ATPase is an integral membrane protein that transports Na+ and K+ across the plasma membrane to establish proper chemical and electrical gradients (for reviews, see Blanco and Mercer, 1998; Therien and Blostein, 2000). Its activity is essential for maintenance of the ...

Up-Regulation of miRNA-21 Expression Promotes Migration and Proliferation of Sca-1+ Cardiac Stem Cells in Mice - Get your full text copy in PDF #895753

Rat Cardiac Stem Cell Media (Serum Free Medium or Medium with Serum). This product is tissue tested including Stem Cells and is available as 500ml sterile filtered unit. The product is also available as a pack of 6, 500ml unit sizes ...

Congenital heart disease (CHD) represents the most prevalent inborn anomaly. Only a minority of CHD cases are attributed to genetic causes, suggesting a major role of environmental factors. Nonphysiological hypoxia during early pregnancy induces CHD, but the underlying reasons are unknown. Here, we have demonstrated that cells in the mouse heart tube are hypoxic, while cardiac progenitor cells (CPCs) expressing islet 1 (ISL1) in the secondary heart field (SHF) are normoxic. In ISL1+ CPCs, induction of hypoxic responses caused CHD by repressing ...

J41] Kshitiz, M.E. Hubbi, E.H. Ahn, J. Downey, D.H. Kim, S. Rey, J. Afzal, A. Kundo, G.L. Semenza, R. M. Abraham, and A. Levchenko, Matrix rigidity controls endothelial differentiation and morphogenesis of cardiac precursors, Science Signaling vol. 5, Issue. 227, ra41, 2012. [http://stke.sciencemag.org/content/vol5/issue227/cover.dtl (Featured as a Cover Article]; also featured in The matter of the heart Science 336 (6088):1483-84 (2012) and the JHU press release) [http://stke.sciencemag.org/cgi/content/abstract/sigtrans;5/227/ra41 Article ...

J41] Kshitiz, M.E. Hubbi, E.H. Ahn, J. Downey, D.H. Kim, S. Rey, J. Afzal, A. Kundo, G.L. Semenza, R. M. Abraham, and A. Levchenko, Matrix rigidity controls endothelial differentiation and morphogenesis of cardiac precursors, Science Signaling Vol. 5, Issue. 227, ra41, 2012. [http://stke.sciencemag.org/content/vol5/issue227/cover.dtl (Featured as a Cover Article)] [http://stke.sciencemag.org/cgi/content/abstract/sigtrans;5/227/ra41 Article ...

Immunofluorescence assessment of Ref-1 response in cardiac tissue after 4 months following implantation of cardiac stem cells. (A-D) Control and (E-H) the L

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Stem cell therapy has recently emerged as an innovative strategy over conventional cardiovascular treatments to restore cardiac function in patients affected by ischemic heart disease. Various stem cell populations have been tested and their potential for cardiac repair has been analyzed. Embryonic stem cells retain the greatest differentiation potential, but concerns persist with regard to their immunogenic and teratogenic effects. Although adult somatic stem cells are not tumourigenic and easier to use in an autologous setting, they exist in small numbers and possess reduced differentiation potential. Traditionally the heart was considered to be a post-mitotic organ; however, this dogma has recently been challenged with the identification of a reservoir of resident stem cells, defined as cardiac progenitor cells (CPCs). These endogenous progenitors may represent the best candidates for cardiovascular cell therapy, as they are tissue-specific, often pre-committed to a cardiac fate, and display a

Aconitine (ACO), a highly toxic diterpenoid alkaloid, is recognized to have effects on cardiac voltage-gated Na+ channels. However, it remains unknown whether it has any effects on K+ currents. The effects of ACO on ion currents in differentiated clonal cardiac (H9c2) cells and in cultured neonatal rat ventricular myocytes were investigated in this study. In H9c2 cells, ACO suppressed ultrarapid-delayed rectifier K+ current (IKur) in a time- and concentration-dependent fashion. The IC50 value for ACO-induced inhibition of IKur was 1.4μM. ACO could accelerate the inactivation of IKur with no change in the activation time constant of this current. Steady-state inactivation curve of IKur during exposure to ACO could be demonstrated. Recovery from block by ACO was fitted by a single-exponential function. The inhibition of IKur by ACO could still be observed in H9c2 cells preincubated with ruthenium red (30μM). Intracellular dialysis with ACO (30μM) had no effects on IKur. IKur elicited by ...

TY - JOUR. T1 - β1-integrin is a cell-autonomous factor mediating the Numb pathway for cardiac progenitor maintenance. AU - Gibbs, Brian C.. AU - Shenje, Lincoln. AU - Andersen, Peter. AU - Miyamoto, Matthew. AU - Kwon, Chulan. PY - 2018/1/1. Y1 - 2018/1/1. N2 - Proper control of multipotent/stem cell number and fate is essential for ensuing organ formation during development. β1-integrin, a subfamily of cell surface receptors, has a conserved role in maintenance of multipotent/stem cells, including renal progenitor cells, follicle stem cells, epidermal stem cells and neural stem cells. However, it remains unclear whether β1-integrin has a role in cardiac progenitor cell (CPC) development. Here we show that a mesodermal deletion of β1-integrin decreases Isl1+ cell number in the second pharyngeal arch (PA2), where CPCs undergo renewal and expansion. Mesp1 lineage-specific mosaicism revealed that β1-integrin-deleted Isl1+ cells do not proliferate in the PA2. Consistently, β1-integrin-deleted ...

TY - JOUR. T1 - Matrix metalloproteinase-1 induces cleavage of exogenous alphab-crystallin transduced by a cell-penetrating peptide. AU - Yang, Seung Won. AU - Lee, Seung Min. AU - Choi, Eun Young. AU - Lee, Kyung Hye. AU - Kim, Soo Hyuk. AU - Shin, Min Jeong. AU - Han, Ye Sun. AU - Kang, Seok Min. AU - Chung, Ji Hyung. PY - 2011/9/1. Y1 - 2011/9/1. N2 - Cell-penetrating peptides (CPPs), including TAT-CPP, have been used to deliver exogenous proteins into living cells. Although a number of proteins fused to TAT-CPP can be delivered into various cells, little is known about the proteolytic cleavage of TAT-fusion proteins in cells. In this study, we demonstrate that a small heat shock protein (sHSP), alphaB-crystallin (αB-crystallin), delivered by TAT-CPP is susceptible to proteolytic cleavage by matrix metalloproteinase-1 (MMP-1) in cardiac myoblast H9c2 cells. Recombinant TAT-αB-crystallin was efficiently transduced into H9c2 cells. For a few hours following protein transduction, generation of ...

Aging is a major risk factor for cardiovascular disease. Although the impact of aging has been extensively studied, little is known regarding the aging processes in cells of the heart. Here we analyzed the transcriptomes of hearts of 12-week-old and 18-month-old mice by single-nucleus RNA-sequencing. Among all cell types, aged fibroblasts showed most significant differential gene expression, increased RNA dynamics, and network entropy. Aged fibroblasts exhibited significantly changed expression patterns of inflammatory, extracellular matrix organization angiogenesis, and osteogenic genes. Functional analyses indicated deterioration of paracrine signatures between fibroblasts and endothelial cells in old hearts. Aged heart-derived fibroblasts had impaired endothelial cell angiogenesis and autophagy and augmented proinflammatory response. In particular, expression of Serpine1 and Serpine2 were significantly increased and secreted by old fibroblasts to exert antiangiogenic effects on endothelial ...

Scientists have a new way to treat heart attacks, and it involves providing cardiac stem cells with what is essentially a road map to the site of the injur | Cardiology

Francis J. Manasek; THE APPEARANCE OF GRANULES IN THE GOLGI COMPLEX OF EMBRYONIC CARDIAC MYOCYTES . J Cell Biol 1 December 1969; 43 (3): 605-610. doi: https://doi.org/10.1083/jcb.43.3.605. Download citation file:. ...

To regenerate heart tissue after a myocardial infarction, researchers have investigated cell therapies involving cardiac progenitor cells. These cells can differentiate into heart cells such as cardiac myocytes, endothelial cells and fibroblasts, but it is not clear whether this differentiation is the sole or even main

Introduction: The role of erythropoietin (Epo) in myocardial repair after infarction remains inconclusive. We observed high Epo receptor (EPOR) expression in cardiac progenitor cells (CPCs). Therefore, we aimed to characterize these cells and elucida

Intra-peritoneal injection and oral gavage on mice; organs and embryos harvesting. - Preparation of primary cultures (cardiac explants) and growth of cardiac progenitor cells and mESCs. - Langendorff preparation to disperse cells from the heart. - Cloning and transfection of cells in culture. - Cellular characterization by Immunohistochemistry, Immunofluorescence and FACS sorting. - Proteins extraction and Western Blotting. - RNA and DNA extraction and purification. - PCR (Polymerase Chain Reaction)/ RT-PCR (Sybr Green and Taqman). - Bacterial cultures and bacterial transformation Good knowledge of Bioinformatic tools. Use of ImageJ, OligoExplorer, BLAST. Basic knowledge of CorelDraw, CLUSTALW, Rasmol v.2.6, Swiss Pdb Viewer, Clone, Gene Runner. ...

LITTLE FALLS, N.J. -- The earliest master stem cells that form the three major cell types in the human heart have been identified and isolated, researchers said.

1 X Phosphate-Buffered Saline Solution (PBS) with Calcium & Magnesium - Sterile filtered & tissue culture tested, including Stem Cells. Pack of 12 (500ml) Bottles. Specifically formulated for Cardiac Stem Cell extraction.. ...

T75 plated Cells $750.00 shipped at room temperature. Cat#36099-26-T75. 120 Population doublings or up to 12 passages.. This product would require pre-coated flasks with Human Adult Cardiac Stem Cell Extra-cellular Matrix Cat# E36099-26 and Human Adult Cardiac Stem Cell Media Cat# M36099-26. Source: Human Adult Cardiac tissue.. Positive Markers: Troponin, α Actin, MEF 2C transcription factor, Sca-1+, c-kit+, GATA &. CD34+, Tropomyosin (upon differentiation), Nkx2.5, KDR, HAND1, ISL 1. For non-academic use please inquire for pricing.. Cells are only guaranteed with purchase of Celprogen Media and Celprogen Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.. ...

The objective of this study was to determine whether aging of the heart is characterized by alterations in the cellular composition of cardiac niches and defective cardiomyogenesis. By employing a morphometric approach in which the three-dimensional structure of each niche was evaluated, we examined 40 and 20 cardiac niches in young and old Fischer 344 rats, respectively. Although a niche can include a single stem cell, clusters composed of three or more cardiac stem cells (CSCs) and early lineage committed cells (LCCs) were included in the analysis. LCCs expressed the stem cell antigen c-kit in combination with the marker of cardiac commitment GATA4. The average volume of the niches did not vary with age. Surprisingly, the number of lineage-negative CSCs increased from 4 to 28 months. Young niches contained 6 CSCs while old niches showed 10 CSCs. However, the fraction of CSCs positive for the senescence-associated marker p16INK4a increased 14-fold with age, from 6% to 85%. Corresponding values ...

The purpose of this study is to investigate the safety of intracoronary cardiac stem cells (CSCs) therapy in humans. Currently, there is no effective intervention to regenerate (regrow) dead heart muscle after a heart attack.. The central hypothesis is that CSCs infused into nonviable myocardial segments will regenerate infarcted myocardium by differentiating into cardiomyocytes and other cell types. According to our hypothesis, CSC infusion regenerates myocardium with consequent improvement in contractile function of the heart and general clinical status. ...

There is great interest in the development of cardiac stem cells (CSCs) cell-based therapeutics; thus, clinical translation requires an efficient method for attaining therapeutic quantities of these cells. Furthermore, an in vitro model to investigate the mechanisms regulating the cardiac homeostasis is crucial. We sought to develop a simple myocardial culture method for enabling both the recapitulation of myocardial homeostasis and the simultaneous isolation of CSCs. The intact myocardial fragments were encapsulated 3-dimensionally into the fibrin and cultured under dynamic conditions. The fibrin provided secure physical support and substratum to the myocardium, which mediated integrin-mediated cell signaling that allowed in situ renewal, outgrowth and cardiomyogenic differentiation of CSCs, mimicking myocardial homeostasis. Since our culture maintained the myocardial CSCs niches, it was possible to define the identity of in vitro renewed CSCs that situated in the interstitium between ...

Celprogen, it would seem, is ahead of many research labs worldwide---and probably the envy of many---as they have produced a 3D printed heart created with a PLA scaffolding that holds adult cardiac stem cells. This technology may indeed one day pave the way for 3D printed organs that can actually be used in transplants. Working toward this cause, the Celprogen team is also in the process of making their own 3D printer so that they will have full control over the cells and resulting bioprints.

Loss and gain-of-function investigation uncovers a regulatory network controlling human heart chamber specification in which the cardiac precursor gene ISL1 accelerates ventricular induction and antagonizes retinoic acid-driven atrial commitment.

Background: APJ (also called AGTRL1, angiotensin II receptor-like 1) is a G- protein-coupled receptor that mediates diverse physiological effects of the neuropeptide apelin. It plays a role in the central and peripheral regulation of the cardiovascular system, in water and food intake, and possibly in immune function. APJ is highly expressed in the cardiovascular system and exerts the hypotensive effect in vivo and plays a counter-regulatory role against the repressor action of angiotensin II. APJ is also found to be a coreceptor for the entry of several HIV-1 and SIV strains, and apelin blocks the entry of HIV-1 and HIV-2.. Application: cAMP assays ...

AORTIC ARCH ANOMALIES. Dr.Santhosh Narayanan. Topic outline. Embryology Anatomy Classification Individual anomalies. Embryology-Sequence of Events. Day I8 - Cardiac precursor cells seen in the form of blood islands Day 20 - First intraembryonic blood vessels Slideshow 1406753 by gwyneth

Immunofluorescence assessment of the combined effect of cardiac stem cells on the expression of SDF-1 and myosin in myocardium after 4 months following implanta