Objective: To analyse the prevalence of Mycoplasma genitalium infection in a population-based study among sexually active married women from a demographic surveillance site in a rural geographical area of Vietnam.. Materials and Methods: Women, 18-49 years of age, were randomly selected to participate. DNA was isolated from endocervical swabs sampled from 990 participating women. The M. genitalium MgPa adhesion gene was detected using a real-time PCR with TaqMan probe. Results: Eight (0.8% [95% confidence interval, 0.25-1.35%]) of the included women were infected with M. genitalium. Two of these positive women reported clinical symptoms. One additional M. genitalium positive but symptom-free woman, however, showed clinical signs of vaginitis. None of the M. genitalium positive women was concomitantly infected with Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum or HIV. Furthermore, there was no obvious association between M. genitalium infection and vaginal douching, use of ...
Objectives: To evaluate the microbiological cure rate after treatment with tetracyclines or azithromycin in patients infected with M genitalium.. Methods: One hundred and fifty-two men and 60 women positive for M genitalium were recruited. Patients treated either with doxycyline for 9 days or with azithromycin 1 g stat. were compared. Those still positive for M genitalium after primary doxycycline treatment received an extended course of azithromycin 500 mg on day 1 followed by 250 mg daily for the following 4 days, whereas those with treatment failure after azithromycin received doxycycline 100 mg twice daily for 15 days.. Results: The eradication rate after azithromycin 1 g stat. was 85% (95% CI 69 to 94) in men (n = 39) and 88% (95% CI 64 to 99) in women (n = 17) and after doxycycline 17% (95% CI 9 to 27) in men (n = 76) and 37% (95% CI 19 to 58) in women (n = 27). Extended azithromycin eradicated M genitalium from 96% (95% CI 85 to 99) of the men (n = 47) and from all six women who failed on ...
Mycoplasma genitalium ATCC ® 33530D™ Designation: Genomic DNA from Mycoplasma genitalium Strain G37 TypeStrain=False Application:
The microbe Mycoplasma genitalium has in several studies been proposed as an individual cause of non-gonococcal urethritis (NGU) in men, and has been associated with pelvic inflammatory disease (PID) and salpingitis. The prevalence of M genitalium has generally been 50-90% of the prevalence of C trachomatis, and this seems to be the case in Sweden as well. This is the first study of the pathogenesis and prevalence of M genitalium in northern Sweden. In total 823 samples, 340 from women and 483 from men, were screened for M genitalium by using a PCR method. Thirtythree (4.0%) patients, 13 (3.8%) women and 20 (4.1%) men, were infected by M genitalium. In the same group 60 (7.3%) patients, 16 (4.7%) women and 44 (9.1%) men, were infected by Chlamydia trachomatis. None of the 22 patients that were tested after treatment with azitromycin was still infected.. ...
The prevalence of M. genitalium infection was 3.8% (4/106; 95% confidence interval, 1.0-9.8). Specific sexual behaviors, HIV infection characteristic, and viral hepatitis coinfection were not significantly associated with M. genitalium infection. Among the 4 patients infected with M. genitalium, 3 were asymptomatic and 1 was diagnosed as having an endometritis. She was successfully treated with ofloxacin for 3 weeks. No C. trachomatis infection was detected.. The prevalence of M. genitalium infection seems lower among French HIV+ women in care than in the US population, but age and race distribution differed slightly (41.3 vs. 35.0 years and 42% of nonwhite vs. 84% of African Americans in the US study, respectively).5 This prevalence is similar to the 1% to 3% usually quoted in the general population from high-income countries.7,8 However, our sample size is limited, and further longitudinal studies are needed to better understand the burden of disease.. In conclusion, a systematic screening of ...
Mycoplasma genitalium, a human mycoplasma species has been established as a cause of nongonococcal urethritis (NGU) in men, particularly in Chlamydia trachomatis-negative patients. It was also shown to play a role in cervicitis and pelvic inflammatory disease (PID) in women. Due to difficulty in culturing, and the lack of routine molecular diagnostic tests, many M. genitalium infections are undetected. The purpose of this study was to evaluate three nucleic acid amplification tests (NAATs) i.e. a recently developed Gen-Probe research only transcription mediated amplification (TMA) assay, a conventional polymerase chain reaction (PCR) assay and a real-time PCR (q-PCR) assay for the detection of M. genitalium in urine specimens of men with symptoms of urethritis. To evaluate the three assays, 300 urine specimens were collected between June 2007 and July 2008 from sexually active male patients presenting with discharge (N=94) and/or burning on micturition (N=206) to a private medical practitioner ...
Mycoplasma genitalium may not be a well-known infection, however its ability to spread is just as easy as all other bacterial infections. It is often symptomless, like many sexually transmitted infections, highlighting the importance to get tested. The only way to treat mycoplasma genitalium.
Results: M. genitalium was detected in 7 (7.0%) of specimens of which one positive sample could not be detected with further methods. No resistance associated mutations were seen in the gyrA and parC genes. In 2 isolates the macrolide associated mutation A2059G was seen. SNP typing revealed Sequence Types 1, 2 and 4. Four different types were seen using MG309 VNTR analysis. Typing assigned M. genitalium to 2 major clusters. Genotypic macrolide resistance was found within one of the clusters. ...
Read Also: 7 Of The Most Commonly Transmitted STDs in Africa. Several studies that had identified Mycoplasma as a STI have showed statistically significant increased rates of infection among sexually active women, with rate/risk of infection increasing with 2 or more sexual partners.. Implications of contracting Mycoplasma genitalium infection. 1. It can significantly affect the ability of the infected individual to have children.. 2. It complicate and increase the severity of the symptoms of HIV and other viral infections.. 3. The can lead to the development of ovarian cancers, lymphomas and prostate cancer.. 4. The infection can lead to pre-term birth and spontaneous abortion.. Prevention of sexually transmitted diseases. The most effective means of preventing sexually transmitted disease is abstinence from sex or faithfulness to a faithful sexual partner. Unprotected sex increase the risk of contracting venereal diseases (vd).. publichealth.com.ng ...
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Mycoplasma genitalium (Mg) is a mollicute that causes a range of human urogenital infections. A hallmark of these bacteria is their ability to establish chronic infections that can persist despite completion of appropriate antibiotic therapies and intact and functional immune systems. Intimate adher …
Exhaustive identification of open reading frames in complete genome sequences is a difficult task. It is possible that important genes are missed. In our efforts to reanalyze the intergenic regions of Mycoplasma genitalium and Mycoplasma pneumoniae, we have newly identified a number of new open reading frames (ORFs) in both M. genitalium and M. pneumoniae. The most significant identification was that of a ribonuclease H enzyme in both species which until now has not been identified or assumed absent and interpreted as such. In this paper we discuss the biological importance of RNase H and its evolutionary implication. We also stress the usefulness of our method for identifying new ORFs by reanalyzing intergenic regions of existing ORFs in complete genome sequences.. ...
We developed and validated a real-time quantitative polymerase chain reaction (qPCR) assay to determine Mycoplasma genitalium bacterial load in endocervical swabs, based on amplification of the pdhD gene which encodes dihydrolipoamide dehydrogenase, using the Rotor-Gene platform. We first determined the qPCR assay sensitivity, limit of detection, reproducibility and
Mycoplasma genitalium is an emerging sexually transmitted pathogen. This wall-less microorganism is among the smallest, self-replicating cell known. Its streamlined genome is an appealing model of a minimal cell. Behind this apparent simplicity, its cell membrane hides a complex cytoskeleton that shapes and polarizes the cell. In this way, cells show a differentiated tip structure, known as terminal organelle (TO), which is involved in key processes of its parasitic way of life. Moreover, TO is involved in gliding motility. This unique motility mechanism is related in many aspects of the biology of this microorganism, with especial relevance in pathogenesis. Nevertheless, the mechanics behind it is still poorly characterized. The general aim of the present work is to deepen the understanding of this mechanism by studying the specific contribution of different domains of proteins previously related with the TO or motility. TO is organized around an internal cytoskeletal structure. MG218 protein ...
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Citation. Bott, K., Fraser, C.M., de Bruijn, F.J., Lupski, J.R., Weinstock, G.M.. Mycoplasma genitalium. Bacterial Genomes : Physical structure and analysis. 1998 Jan 01;: 508-519.. ...
This project provides all code and data for the first whole-cell model of the entire life cycle of a living organism, |i|Mycoplasma genitalium|/i|.
This project provides all code and data for the first whole-cell model of the entire life cycle of a living organism, |i|Mycoplasma genitalium|/i|.
HealthTap: Doctor answers on Symptoms, Diagnosis, Treatment, and More: Dr. Howell on alternative treatments for mycoplasma genitalium: This is a very small bacteria associated with urethral symptoms. It is a kind of sexually transmitted disease. It is treatable with a wide range of antibiotics. However, where there is one sdt there may be others. Go get checked out by a clinician.
Pepin, J; Deslandes, S; Frost, E; Andreasen, AA; Solomon, AW; Bailey, R; Mabey, DCW; (2008) Absence of Mycoplasma genitalium in eye samples from a trachoma-endemic area of Tanzania. The British journal of ophthalmology, 92 (3). p. 435. ISSN 0007-1161 DOI: https://doi.org/10.1136/bjo.2007.121954 ...
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Mükoplasmad on väikseimad meditsiiniliselt olulised bakterid. Neil puudub rakusein, mistõttu nad on resistentsed rakuseinale toimivatele antibiootikumidele.
We report significant failure rates (28%, 95% confidence interval 15%-45%) after administering 1 g azithromycin to men with Mycoplasma genitalium-positive nongonococcal urethritis. In vitro evidence supported reduced susceptibility of M. genitalium to macrolides. Moxifloxacin administration resulted in rapid symptom resolution and eradication of infection in all cases. These findings have implications for management of urethritis.
Mycoplasma species may persist as part of the normal human flora of the respiratory system or the genital area. Of seven known mycoplasma species from the genital area, so far only four have been described as pathogenic: Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum und Ureaplasma parvum.. Mycoplasma hominis (M. hominis) mainly colonizes the genital tract of sexually active men and women, however most of the M. hominis described infections have been diagnosed in woman. M. hominis is associated with pelvic inflammatory disease (PID) and may cause infections during or after pregnancy, such as endometritis or neonatal pneumonia. Common symptoms for infections with M. hominis include e.g. frequent urination, yellow discharge or dysuria.. Globally, the prevalence of Mycoplasma genitalium (M. genitalium) ranges between 1- 4 % for men and 1 - 6.4 % for women. In men, M. genitalium may result in non-specific urethritis and is the second most common cause after Chlamydia trachomatis. ...
Infectious Diseases in Obstetrics and Gynecology is a peer-reviewed, Open Access journal that publishes original research, reviews, and clinical studies related to infectious diseases in womens health. Articles on the diagnosis and management of sexually transmitted diseases, genital and urinary tract infections, and infections in pregnancy will be considered, among other subject areas. Emerging research on the microbiome is also particularly welcomed. The journal aims to disseminate research that will improve the care given to women in the face of current and emerging gynecological infectious diseases.
FRIDAY, Feb. 12, 2016 (HealthDay News) -- Theres yet another sexually transmitted infection that doctors and patients need to watch out for -- Mycoplasma genitalium.. New research from England adds to evidence that the bacteria Mycoplasma genitalium, or MG, is transmitted through sexual contact. Until now, researchers werent sure how the often-symptomless infection, identified in the early 1980s, was spread.. But the current study of more than 4,500 British residents found MG prevalent in 1 percent of participants and linked to risky sexual behaviors, such as multiple sex partners and unsafe sexual practices in the prior year.. This finding suggests MG warrants more attention than it has received to date, said epidemiology professor Betsy Foxman, who specializes in infectious diseases at the University of Michigan.. "My impression is that MG is not on the radar of most general practitioners, [but] with a prevalence of 1 percent, this is an infection that physicians should learn more about," ...
Scientifica is a peer-reviewed, Open Access journal that publishes research articles, review articles, and clinical studies covering a wide range of subjects in the life sciences, environmental sciences, health sciences, and medicine. The journals Editorial Board is divided into the 65 subject areas included within the journals scope.
SYDNEY, Australia, April 19, 2018 (GLOBE NEWSWIRE) - SpeeDxs ResistancePlus® MG Positive Control kit has now been listed with the US Food and Drug
The genome of Mycoplasma genitalium is so far the smallest discovered for any free-living organism, so it has been used as a starting point for defining a minimal genome. Transposon mutagenesis and comparison with a second mycoplasma have further narrowed down the list of genes. Now Glass et al. announce the sequencing of a third mycoplasma, the mucosal pathogen Ureaplasma urealyticum, in the 12 October Nature (Nature 2000, 407:757-762). Their results suggest that there is more than one version of a minimal genome, even for organisms living in very similar environments. Although U. urealyticum has homologs for most of the M. genitalium genes that have been proposed to be essential, it lacks the heat shock protein/chaperonins GroEL and GroES (found in all other sequenced microbial genomes) and the cell division protein FtsZ (absent only in a single archaeon and in chlamydia, which divide in host vacuoles). Key energy metabolism genes are also missing, presumably replaced by U. urealyticums ...
A number of commercially produced M. genitalium DNA amplification modalities have been described. M. genitalium-specific dual-priming oligonucleotide primers have been incorporated into a multiplex PCR assay for six sexually transmitted infection (STI) agents, with subsequent detection of end products via auto-capillary electrophoresis (Seeplex STD6 ACE; Seegene, Seoul, South Korea). One evaluation (10), performed on 739 urogenital specimens, was limited by utilization of a monoplex PCR (using the same primer sets) to confirm positive results and by M. genitalium DNA being detected from only two specimens. Kweon et al. (11) evaluated a PCR microarray capable of detecting 13 agents of genitourinary tract infection (STDetect Chip; LabGenomics, Seongnam, South Korea) using a combination of prospective vaginal and urine specimens that was augmented by archived specimens. When compared to direct sequencing of PCR products generated by primers to the MgPa operon, the assay yielded concordance and ...
J. Craig Venters team at JCVI have worked to carry gene synthesis to its most advanced level - the creation of a completely synthetic genome. Initially, the JCVI team synthesized a 582,970-base pair "minimized" genome of Mycoplasma genitalium using a stepwise assembly [27]. The genome was divided into 24 cassettes of about 24 kb each which were synthesized by commercial manufacturers. These overlapping cassettes were enzymatically stitched together into bacterial artificial chromosomes, which were attached in a stepwise fashion using a combination of restriction digestion and in vitro recombination. At the point where the molecules were "quarter genomes", they were transformed into yeast where in vivo recombination machinery assembled the full M. genitalium chromosome. This was only proof of synthesis, however, and not of a synthetic genome useful for supporting a living organism. In 2010, the JCVI team reported the creation of a bacterium controlled by a fully synthetic genome[4]. The 1.08 ...
J. Craig Venters team at JCVI have worked to carry gene synthesis to its most advanced level - the creation of a completely synthetic genome. Initially, the JCVI team synthesized a 582,970-base pair "minimized" genome of Mycoplasma genitalium using a stepwise assembly [29]. The genome was divided into 24 cassettes of about 24 kb each which were synthesized by commercial manufacturers. These overlapping cassettes were enzymatically stitched together into bacterial artificial chromosomes, which were attached in a stepwise fashion using a combination of restriction digestion and in vitro recombination. At the point where the molecules were "quarter genomes", they were transformed into yeast where in vivo recombination machinery assembled the full M. genitalium chromosome. This was only proof of synthesis, however, and not of a synthetic genome useful for supporting a living organism. In 2010, the JCVI team reported the creation of a bacterium controlled by a fully synthetic genome[4]. The 1.08 ...
SpeeDx and Thermo Fisher Scientific have partnered to bring to U.S. markets a molecular diagnostic test for Mycoplasma genitalium (M. genitalium), a treatment-resistant and rapidly-growing sexually transmitted infection (STI).
Even though free-living, mycoplasmas depend on their hosts for key nutrients, including purines, pyrimidines, several amino acids, and sterols, which are incorporated into the membranes of these wall-less bacteria. These requirements reflect their relative genomic simplicity. The 580-kb genome of Mycoplasma genitalium, for example, is comparable in size to that of a large virus.
Research in the Totten group focuses on the molecular biology, pathogenesis, and disease associations of the recently discovered STD pathogen, Mycoplasma genitalium. Their finding that this bacterium can persist for months, if not years, in infected women lead to our hypothesis that this pathogen evades the host immune response in part by antigenically varying two of its immunogenic surface-exposed proteins. Supporting this hypothesis, they have shown that the sequences of the genes encoding these proteins evolve using reciprocal recombination with non-coding homologous DNA distributed throughout its minimal chromosome. Further, contrary to the accepted wisdom that this bacterium contains few regulatory genes, they have shown that recombination leading to antigenic variation is regulated at the transcriptional, post-transcriptional, and translational levels. The novel recombination and regulatory mechanisms of antigenic variation, the biologic significance of the resulting antigenic variants, ...
1. Bacterial sexually transmitted diseases. Main focus Neisseria gonorrhoeae and antimicrobial resistance. Present sidelines Treponema pallidum, Mycoplasma genitalium and shigellosis. 2. Spiorochetal infections: Lyme borreliosis and leptospirosis (and syphilis, see above) ...
Understanding how complex phenotypes arise from individual molecules and their interactions is a primary challenge in biology that computational approaches are poised to tackle. We report a whole-cell computational model of the life cycle of the human pathogen Mycoplasma genitalium that includes all of its molecular components and their interactions. An integrative approach to modeling that combines diverse mathematics enabled the simultaneous inclusion of fundamentally different cellular processes and experimental measurements. Our whole-cell model accounts for all annotated gene functions and was validated against a broad range of data. The model provides insights into many previously unobserved cellular behaviors, including in vivo rates of protein-DNA association and an inverse relationship between the durations of DNA replication initiation and replication. In addition, experimental analysis directed by model predictions identified previously undetected kinetic parameters and biological ...
How do you research bacteria that don’t grow |em>in vitro|/em>? You go straight to ATCC synthetic nucleic acids. Now featuring |em>Mycoplasma genitalium |/em>and |em>Treponema pallidum|/em>!
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Mycoplasma Genitalium is a very tricky sexually transmitted infection. And since the symptoms can be hidden, many people dont know they have it.
According to a 2006 study of sexually transmitted diseases, the bacterium Mycoplasma genitalium is the smallest living cell as of 2014. It measures only 200 to 300 nanometers, according to Wikipedia....
/PRNewswire/ -- SpeeDx Pty. Ltd. announced that a study using a beta version of PlexPCR™ M. genitalium ResistancePlus™ shows strong results in the detection of...
SYDNEY, June 22, 2016 /PRNewswire/ -- SpeeDx Announces Results of M. genitalium ResistancePlus™ Study. Publication in PLOS ONE shows assay detects both...
Recently, I looked at the genome of M. genitalium from the point of view of latent nucleic-acid secondary structure. (Secondary structure refers to the ability of a single strand of DNA or RNA to base-pair with itself.) I probed each gene with a script designed to detect intragenic self-complementing regions of length eleven (so-called 11-mers), the idea being that complementary runs of bases shorter than that might occur at a high rate by chance. Given M. genitaliums base composition stats (adenine being most prevalent, at 36.24% of all bases in protein-coding message regions, thymine being next-most-abundant, at 32.21% of protein-coding bases), the most likely 11-mer, AAAAAAAAAAA, could be expected to occur by chance once every 70,672 bases. In actuality, that particular 11-mer doesnt occur in the M. genitalium genome, but if it did we could expect around 8 occurrences of it in a genome of 528,500 base pairs. Instead, what we actually find are 749 occurrences of complementary 11-mers in ...
Recently, I looked at the genome of M. genitalium from the point of view of latent nucleic-acid secondary structure. (Secondary structure refers to the ability of a single strand of DNA or RNA to base-pair with itself.) I probed each gene with a script designed to detect intragenic self-complementing regions of length eleven (so-called 11-mers), the idea being that complementary runs of bases shorter than that might occur at a high rate by chance. Given M. genitaliums base composition stats (adenine being most prevalent, at 36.24% of all bases in protein-coding message regions, thymine being next-most-abundant, at 32.21% of protein-coding bases), the most likely 11-mer, AAAAAAAAAAA, could be expected to occur by chance once every 70,672 bases. In actuality, that particular 11-mer doesnt occur in the M. genitalium genome, but if it did we could expect around 8 occurrences of it in a genome of 528,500 base pairs. Instead, what we actually find are 749 occurrences of complementary 11-mers in ...
Recently, I looked at the genome of M. genitalium from the point of view of latent nucleic-acid secondary structure. (Secondary structure refers to the ability of a single strand of DNA or RNA to base-pair with itself.) I probed each gene with a script designed to detect intragenic self-complementing regions of length eleven (so-called 11-mers), the idea being that complementary runs of bases shorter than that might occur at a high rate by chance. Given M. genitaliums base composition stats (adenine being most prevalent, at 36.24% of all bases in protein-coding message regions, thymine being next-most-abundant, at 32.21% of protein-coding bases), the most likely 11-mer, AAAAAAAAAAA, could be expected to occur by chance once every 70,672 bases. In actuality, that particular 11-mer doesnt occur in the M. genitalium genome, but if it did we could expect around 8 occurrences of it in a genome of 528,500 base pairs. Instead, what we actually find are 749 occurrences of complementary 11-mers in ...
Engineering a bacterium that thrives with the smallest feasible string of genes is no vanity project and could have important uses, says Richard Kitney
When the J. Craig Venter Institute announced they had created a cell that ran from artificial DNA, Venter said one of the questions they wanted to answer is, "Whats the minimal genome?" What is the smallest amount of instructions that you can have that will allow a cell to be alive? Thats a fascinating question, but unless / until a lot more labs start pursuing it besides the J. Craig Venter Institute, I dont think it qualifies as a ...
OBJECTIVES: Mycoplasma genitalium has been associated with cervicitis, endometritis, and tubal factor infertility. Because the ability of this bacterium to ascend and infect the fallopian tube remains undefined, we performed an investigation to determine the prevalence of M genitalium in fallopian tube, endometrial, and cervical specimens from women laparoscopically diagnosed with acute salpingitis in Nairobi, Kenya. METHODS: Women presenting with pelvic inflammatory disease were laparoscopically diagnosed with salpingitis. Infection with M genitalium in genital specimens was determined by polymerase chain reaction (PCR). RESULTS: Of 123 subjects with acute salpingitis, M genitalium was detected by PCR in the cervix and/or endometrium in nine (7%) participants, and in a single fallopian tube specimen. In addition, those infected with M genitalium were more often HIV infected than women not infected by M genitalium (seven of nine (78%) v 42 of 114 (37%), p,0.03). CONCLUSIONS: M genitalium is able ...
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