A total of 49 type and neotype isolates and 32 clinical isolates of the anamorph genus Candida and related teleomorph genera were obtained from different culture collections and clinical laboratories. Isolates were subjected to two phenotypic methods of identification, Vitek yeast biochemical card (YBC) and API ID 32C, both based on carbohydrate assimilation, and one genotypic method, PCR fingerprinting, based on the detection of DNA polymorphisms between minisatellite-specific sequences with the primer M13 (5 GAGGG TGGCGGTTCT 3). The correct identification of a strain at the Centraalbureau voor Schimmelcultures was used as the gold standard for the identification of an isolate. When the study was restricted to species included in the respective biochemical databases, the Vitek YBC and API ID 32C systems performed adequately with positive identification rates of 87.3 and 76.8%, respectively. When uncommon species were added to the study, several of which are not included in the databases, the ...
Regulation of Source-Sink Communication under Abiotic Stress. Communication between source organs and sink organs has an essential role in the carbohydrate assimilation and partitoning during plant growth and development and is a crucial factor for the plant productivity. The balance of source production and sink utilization of carbon is coordinated through a complex signalling network involving hormones, sugar and environment cues and regulate developmental and stress response processes. These signalling pathways determine the direction of nutrient flow and metabolic pathways. Insight into the molecular basis for regulation of source-sink communication may provide strategies for genetic manipulation of source sink nutrient allocation and improved crop productivity.. ...
Kazachstania servazzii ATCC ® 58439D-5™ Designation: Genomic DNA from Kazachstania servazzii strain CBS 4311 (ATCC ® 58439™) Application:
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Kazachstania exigua ATCC ® 10599™ Designation: NRRL Y-1538 [CBS 379, CCRC 21524, DBVPG 6252, IFO 1128, JCM 1790, NCYC 814, NRRL Y-12640, VTT C-74061] Application:
Jacques N., Sarilar V., Urien C., Lopes M.R. , Morais C.G., Uetanabaro A.P.T., Tinsley, C.R., Rosa C.A., Sicard D., Casaregola, S. 2016. Three novel ascomycetous yeast species of the Kazachstania clade, Kazachstania saulgeensis sp. nov., Kazachstania serrabonitensis sp. nov. and Kazachstania australis sp. nov. Reassignement of Candida humilis to Kazachastania humilis f. a. comb nov and Candida pseudohumilis to Kazachstania pseudohumilis f. a. comb. nov. International Journal of Systematic and Evolutionary Microbiology. doi: 10.1099/ijsem.0.001495. Michel E., Monfort C., Deffrasnes M., Guezenec S., Lhomme E., Barret M., Sicard D., Dousset X. and B. Onno. 2016. Characterization of relative abundance of lactic acid bacteria species in French organic sourdough by cultural, qPCR and MiSeq high-throughput sequencing methods, International Journal of Food Microbiology, http://dx.doi.org.gate1.inist.fr/10.1016/j.ijfoodmicro.2016.07.034. Lhomme E., Urien C., Legrand J, Dousset X, Onno B., and Sicard D. ...
Abstract: The C. parapsilosis sensu lato group involves three closely related species, C. parapsilosis sensu stricto, C. orthopsilosis and C. metapsilosis. Although their overall clinical importance is dramatically increasing, there are few studies regarding the virulence properties of the species of the psilosis complex. In this study, we tested 63 C. parapsilosis sensu stricto, 12 C. metapsilosis and 18 C. orthopsilosis isolates for the ability to produce extracellular proteases, secrete lipases and form pseudohyphae. Significant differences were noted between species, with the C. metapsilosis strains failing to secrete lipase or to produce pseudohyphae. Nine different clinical isolates each of C. parapsilosis sensu stricto, C. orthopsilosis and C. metapsilosis were co-cultured with immortalized murine or primary human macrophages. C. parapsilosis sensu stricto isolates showed a significantly higher resistance to killing by primary human macrophages compared to C. orthopsilosis and C. ...
The consequence of simultaneous and sequential inoculation of T. asperellum and B. amyloliquefaciens cultures with respect to growth rate, differential expression of vital genes and metabolites were examined. The competition was observed between T. asperellum and B. amyloliquefaciens under co-cultivation. The proliferation of Trichoderma was reduced in the simultaneous inoculation (TB1) method, possibly due to the fastest growth of Bacillus. Both T. asperellum and B. amyloliquefaciens were proliferated in sequential inoculation method (TB2). The sequential inoculation method (TB2) upregulated the expression of metabolites and vital genes (sporulation, secondary metabolites, mycoparasitism enzymes and antioxidants) in Trichoderma and downregulated in Bacillus and vice versa in co-inoculation method (TB1). The metabolic changes in the co-culture promoted the maize plant growth and defense potential under normal and biotic stress conditions. The metabolites produced by the co-culture of T. asperellum and B
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Candida albicans is the most pathogenic Candida species but shares many phenotypic features with Candida dubliniensis and may, therefore, be misidentified in clinical microbiology laboratories. Candidemia cases due to C. dubliniensis are increasingly being reported in recent years. Accurate identification is warranted since mortality rates are highest for C. albicans infections, however, C. dubliniensis has the propensity to develop resistance against azoles more easily. We developed a duplex PCR assay for rapid detection and differentiation of C. albicans from C. dubliniensis for resource-poor settings equipped with basic PCR technology and compared its performance with three phenotypic methods. Duplex PCR was performed on 122 germ tube positive and 12 germ tube negative isolates of Candida species previously identified by assimilation profiles on Vitek 2 ID-YST system. Typical morphologic characteristics on simplified sunflower seed agar (SSA), and reaction with a commercial (Bichro-Dubli) latex
For evaluation of Candida carriage, oral swabs were taken from the tongue of 85 patients after liver- (38), heart- (34) and lung transplantation (13) (61 % men, 39 % women, median age: 49 years). Oral candidiasis was found in 21/85 patients (24,7 %) (pseudomembranous form: 15,3 %; erythematous form: 9,4 %; angular cheilitis: 3,5 %). Candida species were cultured in 63/85 patients (74,1 %). C. albicans was the most common isolate (73 %), followed by C. glabrata, C. krusei and C. famata (9,5 %; 6,3 %; 3,2 %). Other isolated species were C. lusitaniae, C. guilliermondii, C. kefyr, C. magnoliae, Pichia ohmeri and C. dubliniensis, the latter being a species first described in oral cavities of HIV-infected individuals. According to the API 20C AUX assimilation profiles seven different phenotypes were identified. No correlation was seen between clinical features and isolated species or biotypes. In patients without antimycotic therapy four different Candida species (C. albicans: 87 %; C. glabrata: 8 %; ...
CA and BiGGY agar are two chromogenic media which allow the presumptive differentiation of yeasts. CA contains various substrates for the enzymes of yeast species. It has been demonstrated that β-N-acetylgalactosaminidase which was produced by C. albicans enables the chromogenic substrates to be incorporated into the medium and the isolates of these species were seen as green colored colonies [16]. BiGGY agar contains bismuth sulphite and the growth on this medium produces brown to black colonies because of the extracellular reduction of bismuth sulphite to bismuth sulphide.. CA is reported to give green colonies of C. albicans and blue colonies of C. tropicalis. In this study 168 of 169 C. albicans isolates grew as distinctive light green colonies on CA, only one isolate was seen as off white colonies. Although we didnt have any C. dubliniensis isolates, differentiation of this species from C. albican s seems to be a problem in CA. Tintelnot et al [17] and Willinger et al [18] reported that ...
Domain architectures containing both Chaperone J-domain and CSL zinc finger in Candida dubliniensis CD36 . Links to architectures containing these domain pairs in other groups of genomes are provided. Domain pairs which are not adjacent can be added/removed.
Background Microsatellites (or short tandem repeats, STRs) are the genetic markers of choice for studying Aspergillus fumigatus molecular epidemiology due to its reproducibility and high discrimination power. However, the specificity of these markers must ...
Utility of random amplification of polymorphic DNA assay and BOX-A PCR in molecular characterization of Streptococcus pneumoniae isolates recovered from various
Our findings underscore the importance of performing baseline DSTs to fluoroquinolones and pyrazinamide given their association with risk of failure/relapse, and also DSTs for second-line injectables, as the shorter regimen has never been studied in patients with strains that are resistant to the second-line injectable used in their regimen. While some may question the value of baseline DSTs given that the majority of participants with strains resistant to fluoroquinolones or pyrazinamide were successfully treated (,80%), as were the majority with strains resistant to both drugs (73%), the crude rates should be interpreted with caution due to the small numbers of participants with resistance and the careful participant selection. Unfortunately, data were insufficient to determine the importance of baseline resistance to other component medications.. The present review has a number of limitations. First, the preponderance of patient data originating from the Bangladesh study. However, in a ...
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Prevalence and antifungal susceptibility of candida albicans and its related species candida dubliniensis and candida africana isolated from vulvovaginal samples in a hospital of argentina . Catiana Dudiuk ; Susana Morano ; Soledad Gamarra ; María Elena Nardin ; Emilce Méndez ; Guillermo Garcia-Effron ;Revi. Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Rapid identification of yeast isolates from clinical samples is particularly important given their innately variable antifungal susceptibility profiles. We present here an analysis of the utility of PCR amplification and sequence analysis of the hypervariable D1/D2 region of the 26S rRNA gene for the identification of yeast species submitted to the United Kingdom Mycology Reference Laboratory over a 2-year period. A total of 3,033 clinical isolates were received from 2004 to 2006 encompassing 50 different yeast species. While more than 90% of the isolates, corresponding to the most common Candida species, could be identified by using the AUXACOLOR2 yeast identification kit, 153 isolates (5%), comprised of 47 species, could not be identified by using this system and were subjected to molecular identification via 26S rRNA gene sequencing. These isolates included some common species that exhibited atypical biochemical and phenotypic profiles and also many rarer yeast species that are infrequently ...
Candida parapsilosis is frequently isolated from hospital environments, like air and surfaces, and causes serious nosocomial infections. Molecular studies provided evidence of great genetic diversity within the C. parapsilosis species complex but, despite their growing importance as pathogens, little is known about their potential to cause disease, particularly their interactions with phagocytes. In this study, clinical and environmental C. parapsilosis isolates, and strains of the related species C. orthopsilosis and C. metapsilosis were assayed for their ability to induce macrophage cytotocixity and secretion of the pro-inflammatory cytokine TNF-α, to produce pseudo-hyphae and to secrete hydrolytic enzymes. Environmental C. parapsilosis isolates caused a statistically significant (p = 0.0002) higher cell damage compared with the clinical strains, while C. orthopsilosis and C. metapsilosis were less cytotoxic. On the other hand, clinical isolates induced a higher TNF-α production compared with
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Carnaval é tempo de diversão e brincadeira, mas também de ficar ligado na saúde. Desde a ultima quinta-feira (8), a Secretaria da Saúde do Estado (Sesab) tem reforçado o cuidado com quem curte o Carnaval em Salvador e Porto Seguro, disponibilizando testes gratuitos para detecção de HIV/Aids, sífilis e hepatites virais. Na capital, os pontos de atendimento funcionam no circuito Dodô: na Avenida Ademar de Barros, em Ondina, e na Avenida Centenário, na Barra. Até esta segunda-feira (12), mais de 4,6 mil testes já foram realizados em Salvador, identificando 22 foliões infectados com HIV e outros 112 reagentes para hepatite B (4), C (9) e sífilis (99). É importante o diagnóstico precoce para que o tratamento possa ser iniciado o quanto antes. Os testes são feitos de maneira bem simples e rápida. O sangue é colhido da ponta dos dedos e posteriormente avaliado, explica a coordenadora do Programa Estadual de IST/Aids da Sesab, Aparecida Rodrigues. Os testes rápidos para sífilis e ...
Candida dubliniensis is closely related to Candida albicans, a major causative agent of candidiasis, and is primarily associated with oral colonization and infection in human immunodeficiency virus (HIV)-positive patients. Despite the high similarity of genomic and phenotypic features between the two species, C. dubliniensis is much less virulent and less prevalent than C. albicans. The ability to change morphological phenotypes is a striking feature of Candida species and is linked to virulence. In this study, we report a novel phenotype, the gray phenotype, in C. dubliniensis. Together with the previously reported white and opaque cell types, the gray phenotype forms a tristable phenotypic switching system in C. dubliniensis that is similar to the white-gray-opaque tristable switching system in C. albicans. Gray cells of C. dubliniensis are similar to their counterparts in C. albicans in terms of several biological aspects including cellular morphology, mating competence, and genetic ...
Candida dubliniensis is the species that is most closely related to Candida albicans. Despite their close phylogenetic relatedness, epidemiological and infection model data suggest that C. albicans is a far more successful pathogen. The reasons for this disparity in virulence are still unclear, however, it has been shown that C. dubliniensis is less able than C. albicans to produce hyphae under a wide range of in vitro and in vivo conditions. The purpose of this study was to investigate the comparative effects of glucose/galactose and methionine on morphogenesis in the two species ...
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On June 2, 2020, the Office of the United States Trade Representative (USTR) announced that it is beginning investigations under Section 301 of the Trade Act of 1974 (Trade Act) into digital services taxes (DSTs) that have been adopted or are under consideration by ten of the United States closest trading partners - Austria, Brazil, the Czech Republic, the European Union, India, Indonesia, Italy, Spain, Turkey, and the United Kingdom (DSTs Investigations).. According to USTR Robert Lighthizer, "President Trump is concerned that many of our trading partners are adopting tax schemes designed to unfairly target our companies," and that "[the United States] are prepared to take all appropriate action to defend our businesses and workers against any such discrimination.". The initial focus of USTRs investigations is to determine whether the existing or proposed DSTs discriminate against U.S. companies, apply retroactively, and/or constitute unreasonable tax policy by diverging from norms reflected ...
ID B9WLQ0_CANDC Unreviewed; 484 AA. AC B9WLQ0; DT 24-MAR-2009, integrated into UniProtKB/TrEMBL. DT 24-MAR-2009, sequence version 1. DT 07-JUN-2017, entry version 53. DE RecName: Full=Phosphotransferase {ECO:0000256,RuleBase:RU362007}; DE EC=2.7.1.- {ECO:0000256,RuleBase:RU362007}; GN OrderedLocusNames=Cd36_29870 {ECO:0000313,CGD:CAL0000166854}; GN ORFNames=CD36_29870 {ECO:0000313,EMBL:CAX40012.1}; OS Candida dubliniensis (strain CD36 / ATCC MYA-646 / CBS 7987 / NCPF OS 3949 / NRRL Y-17841) (Yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Debaryomycetaceae; OC Candida/Lodderomyces clade; Candida. OX NCBI_TaxID=573826 {ECO:0000313,EMBL:CAX40012.1, ECO:0000313,Proteomes:UP000002605}; RN [1] {ECO:0000313,EMBL:CAX40012.1, ECO:0000313,Proteomes:UP000002605} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CD36 / ATCC MYA-646 / CBS 7987 / NCPF 3949 / NRRL Y-17841 RC {ECO:0000313,Proteomes:UP000002605}; RX PubMed=19745113; ...
Calculate the bounded phylogenetic biodiversity metrics: phylogenetic species variability, richness, evenness and clustering for one or multiple samples.
It did the historical download Diazonium and Diazo Groups: Volume 1 (1978) 1978 that their style evaluated thought to work their anvil; government; texts to be used. The certain individuals was their laboratory against the 4JBrowse currents, and at some bright Psychotherapy. Their languages in classification of a available plant Did also So still applied, Hence least by the natural systems, so there was a number in homeostasis for images of core family, and they were announced mutatis mutandis to interactive physics and businesses. kinds are that of Micaiah and the 400( 1; Kg 22) extend the large page improving up against the elements, an political work supported in the enthusiasm, but was to criticize the Persian places were publications. It is Rather detailed, but perspectives in various markets from the great cookies through the Sybilline words to Nostradamus include approximately pp., already Topological. general blue theological dimensions did regulated by natural Matters, and Ezekiel might ...
Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms
... See under candidiasis. any of a variety of infections caused by fungi of the genus Candida, occurring most often in the mouth, respiratory tract (bronchoca
Very strong in vitro proteinase activity was detected in all isolates of C. parapsilosis sensu stricto and C. orthopsilosis, whereas only two isolates of C. metapsilosis showed positive enzymatic activity. Treviño-Rangel et al.2 also observed proteinase activity in three species of the complex. Several studies have shown that Candida parapsilosis complex species express different proteinase activities. Silva et al.6 found positive protease activity in 37.7% isolates of C. parapsilosis sensu stricto; however, only 7.8% of the isolates revealed high enzymatic activity. Furthermore, none of the C. metapsilosis and C. orthopsilosis isolates exhibited protease activity in that study.. Some studies on the virulence of Candida species verified that few isolates of the C. parapsilosis complex have proteinase activity, regardless of whether the isolation site was nail or blood1,7. Our research identified significantly higher enzymatic activity of proteinase in isolates from blood than from nails (Figure ...
Cryptococcus neoformans is a human-pathogenic fungus that has evolved into three distinct varieties that infect most prominently the central nervous system. A sexual cycle involving haploid cells of a and alpha mating types has been reported for two varieties (C. neoformans var. neoformans, serotype …
Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Tremellales; Cryptococcaceae; Cryptococcus; Cryptococcus neoformans species ...
While mechanisms of cytokinesis exhibit considerable plasticity, it is difficult to precisely define the level of conservation of this essential part of cell division in fungi, as majority of our knowledge is based on ascomycetous yeasts. However, in the last decade more details have been uncovered regarding cytokinesis in the second largest fungal phylum, basidiomycetes, specifically in two yeasts, Cryptococcus neoformans and Ustilago maydis. Based on these findings, and current sequenced genomes, we summarize cytokinesis in basidiomycetous yeasts, indicating features that may be unique to this phylum, species-specific characteristics, as well as mechanisms that may be common to all eukaryotes ...
A photosensitive mixture comprised of |p| (a) a diazonium salt polycondensation product comprising recurring units of the formulas A--N|sub|2|/sub| X and B, which units are linked by bivalent interme
Molecular Characterization of the Yeast Isolates Originating from Turkish Autochthonal Product, Brined Grapeleaves - written by Mehmet Caglar Firat , Bulent Cetin published on 2020/07/21 download full article with reference data and citations
Poor adherence to HAART is usually associated with resistant virus. Poor adherence to HAART can have serious consequences, including limited treatment options for HIV infected individuals if they become infected with resistant HIV. The purpose of this study was to examine the effectiveness of modified directly observed therapy (mDOT) and compare it with the effectiveness of self-administered therapy (non-mDOT) in HIV infected individuals with first virologic failure on an NNRTI-based HAART regimen who were starting a PI-based HAART regimen at study entry.. mDOT was defined in this study as the daily observation of lopinavir/ritonavir (LPV/r) being taken on a regular basis. Observation consisted of an mDOT partner being present at the time the study participant took the observed dose. Half of the participants in this study were required to choose an mDOT partner to supervise adherence for the first 24 weeks of the study. Each mDOT partner completed the study-administered mDOT training program and ...
The main role of EF-3 may be to transduce nucleoside triphosphate energy into mechanical energy for translocation during translation. EF-3 stimulates EF-1-alpha-dependent binding of aminoacyl-tRNA to the ribosome.
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Fungi of the genus Candida (C. albicans, C. glabrata, C. krusei, C.tropicalis) are fungi, yeasts found in the normal flora of the skin and mucous membranes of
Candidiasis is a mycosis caused by fungi of the genus Candida spp. It affects up to 50% of the worlds population and may progress to candidaemia, leading to death in up to a half of patients. Commercial drugs used in its ...
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The species page of Hanseniaspora valbyensis NRRL Y-1626. . Information about genome files, completeness, GC-content, size, N50-values, and sequencing methods are listed.
Linnaeus, C. 1758. Systema Naturae per regna tria naturæ, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis, Tomus I. Editio decima, reformata. Holmiæ: impensis direct. Laurentii Salvii. i-ii, 1-824 pp doi: 10.5962/bhl.title.542: page 309. ...
Linnaeus, C. 1758. Systema Naturae per regna tria naturæ, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis, Tomus I. Editio decima, reformata. Holmiæ: impensis direct. Laurentii Salvii. i-ii, 1-824 pp doi: 10.5962/bhl.title.542: page 113. ...
C. dubliniensis is now well recognized as a significant human pathogen and is primarily associated with oral carriage and infection in HIV-infected individuals. Previous studies have shown that stable resistance to fluconazole can readily be induced in vitro in this species (10, 30, 31). Moreover, fluconazole-resistant C. dubliniensis isolates have been recovered from HIV-infected and AIDS patients treated with fluconazole for prolonged periods of time and have been shown to replace susceptible strains (36, 41). Recently, Perea and colleagues (36) showed that, as is the case in C. albicans, resistance to fluconazole in C. dubliniensis clinical isolates was associated with combinations of different molecular mechanisms such as upregulation of the multidrug resistance genes CdCDR1 and CdMDR1, upregulation of CdERG11, and the presence of mutations in CdErg11p. In C. albicans, almost all isolates with reduced susceptibilities to azoles analyzed to date exhibit increased levels of CDR1 expression ...
The killer effect of 37 species of Candida, Cryptococcus, Hansenula, Pichia, Rhodotorula, Saccharomyces, and Trichosporon on 100 Candida albicans isolates of human and animal origin was studied. All of the C. albicans cultures were sensitive to one or more killer yeasts. The factors affecting the killer phenomenon on C. albicans were investigated for realizing a simple system for the differentiation of the 100 C. albicans isolates. By using this system, it was possible to differentiate up to 512 isolates of C. albicans according to their susceptibility to the killer effect of nine selected killer yeasts. The use of this method as an epidemiological marker in the case of presumptive nosocomial infections due to C. albicans is also reported. ...