... - Site-specific mutagenesis techniques, also known as site-directed mutagenesis .. GeneTailor Site-Directed mutagenesis system (Invitrogen. I have designed
Webb, Helen M. and Sixma, T.K. and Hol, W.G.J. and Hirst, Timothy R. (1994) Analysis by Site-Directed Mutagenesis of Important Residues Invoved in the Assembly of Escherichia-Coli Heart-Labile NALYSIS BY SITE-DIRECTED MUTAGENESIS OF IMPORTANT RESIDUES Enterotoxin. In: 6th European Workshop on Bacterial Protein Toxins, Stirling, Scotland. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) ...
There is a lack of information about structural requirements for a functional transmembrane domain in eukaryotic membrane proteins. This led me to design a eukaryotic system to bring more information about this structure and the role of the positively charged residues situated at the cytoplasmic side of a transmembrane region. The CD2 molecule was chosen as a model for an integral membrane protein. I deleted the transmembrane domain (26 amino acids) by oligonucleotide site-directed mutagenesis and overlap extension using PCR mutagenesis. Truncated forms with transmembrane regions 14, 12, 10 and 8 amino acids long were created. The common positive cluster (Lys-Arg-Lys-Lys) at the cytoplasmic domain was disrupted by substituting it for polar residues (Gln-Gln-Gln-Gln). The effects of such mutagenesis was examined after expression of the mutant proteins in eukaryotic cells (COS-7 and CHO) . Cellular localization was determined by panning and immunostaining experiments. The functional state of the ...
Creative Biostructure develops comprehensive protein evolution and engineering platform to generate gene diversification by site-directed mutagenesis libraries.
what are the criteria for good primers in a pcr reaction?describe how you would use site-directed mutagenesis to change, Hire Biology Expert, Ask Academics Expert, Assignment Help, Homework Help, Textbooks Solutions
Biomigas site-saturation mutagenesis technology systematically substitutes wildtype amino acids with partial or all 19 non-wildtype mutants.. Advantages. ...
The ion selectivity of the Na+ channel is determined by a ring of amino acids created by the P-loops of all four domains (D1-D4), and the major determinants are the residues referred to as `DEKA (Asp372, Glu898, Lys1419, and Ala1711; following residue numbers are the equivalents of human Nav1.5) (Heinemann et al., 1992; Sun et al., 1997) (Fig. 6A). However, the contribution of each P-loop to the ion selectivity is not equal (Schlief et al., 1996). Site-directed mutagenesis studies have shown that the positive Lys residue in D3 is the most critical residue for ion selectivity (Chiamvimonvat et al., 1996). In contrast, the physiological role of the D4 selectivity filter region is less clear. Cysteine mutagenesis experiments have shown that the five residues between Ser1710 and Asp1714 around the selectivity filter residue Ala1711 are all accessible from outside (Chiamvimonvat et al., 1996), suggesting that these five residues face the hydrophilic ion-conducting pore. In fact, mutations at the ...
Amino acids within TMs have been shown to play important roles in substrate binding, maintenance of protein stability, and correct folding of proteins (Hong et al., 2004, 2010; Gui and Hagenbuch, 2009; Miyagawa et al., 2009; Li et al., 2012). Studies of single nucleotide polymorphisms have also pointed out that mutants located within TMs often result in functional changes (Kalliokoski and Niemi, 2009). In the present study, we used site-directed mutagenesis to study the involvement of amino acid residues within the putative TM6 of OATP1B1 in substrate transport. Of the 24 amino acids analyzed, F262A, V264A, L267A, S269A, F276A, and F278A showed a more than 60% reduction of transport activity. Further studies revealed that these mutants had much reduced transporter protein expression compared with the wild-type. If protein expression is considered in the comparison of the transport activity, E-3-S uptake by these mutants was more than 60% of that by wild-type OATP1B1 (data not shown). Therefore, ...
Site-directed mutagenesis, cloning, strains, and media: Four mutant alleles of SSA1, ssa1-101 (K69Q), ssa1-102 (G198D), ssa1-103 (G199D), and ssa1-104 (G226D), were created using the Quick-Change site-directed mutagenesis kit (Stratagene, La Jolla, CA). SSA1 inserted between the HindIII and BamHI sites of YEp351 (Hillet al. 1986; YEp351-SSA1 plasmid provided by E. Craig, University of Wisconsin, Madison) served as the template. The primer pairs used were as follows: for ssa1-101, 5′-CCGTTTTCGACGCTCAGCGTTTGATCGG-3′ and 5′-CCGATCAAACGCTGAGCGTCGAAAACGG-3′; for ssa1-102, 5′-GTCTTGATTTTCGACTTGGATGGTGGTACTTTCG-3′ and 5′-CGAAAGTACCACCATCCAAGTCGAAAATCAA GAC-3′; for ssa1-103,5′-GATTTTCGACTTGGGTGATGG TACTTTC GATGTC-3′ and 5′-GACATCGAAAGTACCATCACCCAAGTC GAAAATC-3′; and for ssa1-104, 5′-GGTGACACCCATTTGGAT GGTGAAGATTTTGAC-3′ and 5′-GTCAAAATCTTCACCATC CAAATGGGTGTCACC-3′. In each primer the altered nucleotide is boldfaced and underlined. Mutagenesis resulted in plasmids ...
Our lab (at least Amanda and Janet) have not tried it yet. Our understanding is that it is for more substantial changes than just single amino acid changes ...
This page is beta. Please feel free to update it. The idea here is to create a flow of work to simulate the point mutation of a single amino acid in a protein. ...
you will bite yourself in the a** when you later discover that there was a mutation somewhere else in your sequence. Considering the cost of commercial sequencing (the cheapest way are those packages where they just run the reaction and you get the raw data and do the evaluation by yourself, there is free software available like finch tv), sequencing your constructs is a must before you derive any data from them that you want to publish/patent or which are important in any other context. I had learned my lesson once by finding out almost too late that one of the primers I had used was missing a base (still in the time of running your own DNA synthesizer and sequencing with gels and 35S) my 2c Wo On 15 Aug., 04:05, mnr mnr ,mnr... from gmail.com, wrote: , If I were to go down the Phusion road, will I need to sequence the entire of , my gene (4.5 kbp) or just the mutated region to make sure the protein , finally has mutated in the right places? ...
Ortsgerichtete Mutagenese: Punktmutationen, Aminosäure-Austausche und Insertionen einführen. Bis zu 150 bp. Kurze Lieferzeit. Absolute Vertraulichkeit.
Site-specific mutagenesis techniques are aimed at the precise substitution, insertion or deletion of any coding sequence in vitro. More recently, however, such precise alterations are also being developed for in vivo gene/genome modifications. These techniques are revolutionizing our understanding of the genetic and molecular mechanisms in several biological systems, which could lead to the development of new enzymes, therapeutics as well as improved agricultural applications. ...
Deadline: 4 June 2020 EFSAs GMO Panel has launched an open consultation on its draft scientific opinion on the applicability of the EFSA opinion on site-directed nucleases type 3 (SDN-3) for the safety assessment of plants developed using site-dire .... ...
Older research outputs will score higher simply because theyve had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 271,050 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 61% of its contemporaries ...
G-protein-coupled receptors generally share a similar structure containing seven membrane-spanning domains and extracellular site(s) for N-glycosylation. The histamine H2 receptor is a member of the family of G-protein-coupled receptors, and has three extracellular potential sites for N-glycosylation (Asn-4, Asn-162 and Asn-168). To date, however, no information has been presented regarding N-glycosylation of the H2 receptor. To investigate the presence, location and functional roles of N-glycosylation of the H2 receptor, site-directed mutagenesis was performed to eliminate the potential site(s) for N-glycosylation singly and collectively. The wild-type and mutated H2 receptors were expressed stably in Chinese hamster ovary (CHO) cells or transiently in COS7 cells. Immunoblotting of the wild-type and mutated H2 receptors with an antiserum directed against the C-terminus of the H2 receptor showed that mutation at Asn-162, but not at Asn-168, resulted in a substantial decrease in the molecular ...
Diacylglycerol kinases (DAGKs) catalyse ATP-dependent phosphorylation of sn-1,2-diacylglycerol that arises during stimulated phosphatidylinositol turnover. DAGKα is activated in vitro by Ca2+ and by acidic phospholipids. The regulatory region of DAGKα includes an N-terminal RVH motif and EF hands that mediate Ca2+-dependent activation. DAGKα also contains tandem C1 protein kinase C homology domains. We utilized yeast, Saccharomyces cerevisiae, which lacks an endogenous DAGK, to express DAGKα and to determine the enzymic activities of different mutant forms of pig DAGKα in vitro. Six aspartate residues conserved in all DAGKs were individually examined by site-directed mutagenesis. Five of these aspartate residues reside in conserved blocks that correspond to sequences in the catalytic site of phosphofructokinases. Mutation of D434 (Asp434) or D650 abolished all DAGKα activity, whereas substitution of one among D465, D497, D529 and D697 decreased the activity to 6% or less of that for ...
GENEWIZ can increase your research productivity by performing your time-consuming site-directed mutagenesis projects efficiently and cost-effectively. Our customized mutagenesis services provide a fail-safe approach to obtain mutant constructs quickly, with 100% accuracy, thus eliminating the possibility of undesired mutations in your gene.. ...
TY - JOUR. T1 - Plasmid-based one-pot saturation mutagenesis. AU - Wrenbeck,Emily E.. AU - Klesmith,Justin R.. AU - Stapleton,James A.. AU - Adeniran,Adebola. AU - Tyo,Keith E.J.. AU - Whitehead,Timothy A.. PY - 2016/11/1. Y1 - 2016/11/1. N2 - Deep mutational scanning is a foundational tool for addressing the functional consequences of large numbers of mutants, but a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Here we present nicking mutagenesis, a robust, single-day, one-pot saturation mutagenesis method performed on routinely prepped plasmid dsDNA. The method can be used to produce comprehensive or single- or multi-site saturation mutagenesis libraries.. AB - Deep mutational scanning is a foundational tool for addressing the functional consequences of large numbers of mutants, but a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Here we present nicking mutagenesis, a robust, ...
Defining fibronectins cell adhesion synergy site by site-directed mutagenesis.s profile, publications, research topics, and co-authors
The drug binding also produces significant displacement of several segments of the backbone. There is a large movement of the segment between Val107 and Leu113 (including the mutated residue Ala109), apparently because the segment between Gln104 and Gln108 becomes helical as a result of drug binding. Some of the side chain atoms appear to become displaced by nearly 3 Å upon drug binding. In addition, the segment between Gly861 and Gly870, just preceding TM helix 8, shows a slight backbone displacement and side chain movement up to 3 Å.. Similar interactions often involving the same set of residues appear to occur with the binding of R6G and Et in our model (not shown). Surprisingly, no acidic residue that would neutralize the positive charges of these dyes was found within 6 Å of the ligand (except Asp566, which is close to the benzoic acid moiety of R6G but more than 11 Å away from its amine nitrogens), although the partially negative π-electron cloud of the phenyl ring of Phe664 is about ...
1RQL: X-ray crystallographic and site-directed mutagenesis analysis of the mechanism of Schiff-base formation in phosphonoacetaldehyde hydrolase catalysis
Site-directed mutagenesis of a sequence to make specific, targeted changes to double stranded DNA. Based on optimised protocols, BaseGene is able to create mutations in virtually every DNA fragment. This is a valuable tool for studying DNA or protein structure and function. Site-directed or random mutagenesis can be applied to any DNA-fragment cloned into a plasmid vector. We can help you to introduce a range of mutations, varying from point mutations to deletions or insertions.. ...
Directed evolution has so far been used almost exclusively as a tool for engineering proteins. By mutation techniques such as site-directed mutagenesis, cassette-mutagenesis, random mutagenesis, and error prone PCR variants of protein functions have been generated and the libraries thus produced have been screened for their ability to perform a specific function. Recursive application of this procedure has been used successfully for the modification of physical and catalytic properties of enzymes such as pH optima, thermo-tolerance, solvent stability, stereoselectivity, catalytic activity and substrate specificity as well as toxicity resistance mechanisms in bacteria and the host range and stability of viruses.Traditional mutagenesis approaches for evolving new properties in enzymes have a number of limitations. These approaches are only applicable to genes or sequences that have been cloned and functionally characterized and that have a discrete function. Also, the traditional mutagenesis ...
mutagenesis expert come in~ - posted in Molecular Cloning: I want to do a site directed mutagenesis in a 6kb plasmid, the primer set is primerF: GCCTCTGCTTAGTGGaaTTcCCACCCCCACAACCCGCAGG primerR: CCTGCGGGTTGTGGGGGTGGgAAttCCACTAAGCAGAGGC, The reaction system was designed as following: template 1ul (100ng) primer 1+1 ul (10mM each) dNTPs 1 ul (25mM each) 10Xpfu buffer 5 ul pfu 1 ul H2O...
G. DeSantis and J.B. Jones, Combining Site-Specific Chemical Modification with Site-Directed Mutagenesis: A Versatile Strategy To Move Beyond the Structural Limitations of the 20 Natural Amino Acids Side Chains in Protein Engineering , in In Vitro Mutagenesis Protocols , Methods in Molecular Biology, Humana Press, 2002, vol 182, pp55-65 ...
Weird problem in in vitro mutagenesis - posted in Molecular Biology: Hi, Iam doing invitro mutagenesis for a 5.2Kb plasmid using stratagene kit. I did not get any results with my plasmid, so checked the control in the kit. Unfortunately the control is not working. I have tried many times, but got no colonies. The conditions used were according to the instructions given by the manufacturer. 95 C 50 sec 95 C 50 seconds 55 C 1 min 68 C 5 minute x 18 times 68 C 5 minutes. I even tried addin...
For contributions to the development of methods within DNA-based chemistry [specifically, for his fundamental contributions to the establishment of oligonucleotide-based, site-directed mutagenesis and its development for protein studies ...
Kajohn Boonrod is the author of this article in the Journal of Visualized Experiments: Homemade Site Directed Mutagenesis of Whole Plasmids
Cysteine is the ubiquitous ligand of iron-sulfur clusters in proteins, although chemical models have indicated that functional groups other than thiolates can coordinate iron in iron-sulfur...
Identification of Critical Amino Acid Residues in Human Immunodeficiency Virus Type 1 IN Required for Efficient Proviral DNA Formation at Steps prior to Integra
Plasmids ME1646 (S29, nucleotide 2555 A → G; amino acid 853 H → R), ME1647 (S41, nucleotide 2555 A → G; amino acid 853 H → R), and ME1648 (S55, nucleotides 2260 A → G, 2545 G → A; amino acids 754 N → D, 849 A → T) were sequenced between the DraI and HpaI sites to determine the mutations responsible for the mutant phenotypes. Site-directed mutagenesis was performed to generate the single (S29/S41) and double mutations (S55) using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA) as recommended. The primers used were: S29/S41 P126, 5′-CAG AAT TGC TGA TTT TCG TTT AGA TAA ACC ATT C, and P127, 5′-G AAT GGT TTA TCT AAA TCA CGA AAA TCA GCA ATT CTG; S55 (nucleotide 2260 A → G) P135, 5′-TTG TGA TTT ATA GGG ATG TGG GGA ATA TTG, and P136, 5′-CAA TAT TCC CCA CAT CCC TAT AAA TCA CAA; and S55 (nucleotide 2545 G → A) P132, 5′-TAT TCA AAT GCC AGA ATT ACT GAT TTT CAT GAT TTA G, and P133, 5′-C TAA ATC ATG AAA ATC AGT AAT TCT GGC ATT TGA ATA (the boldface ...
Mechano-growth factor (MGF), an alternative splicing variant of insulin-like growth factor-1 (IGF-1) gene, promotes cell proliferation and inhibits cell differentiation. It also plays an important role in tumor development. It is important to optimize the production process and achieve MGF protein because there is no commercial MGF protein available. In this study, the human MGF gene is cloned into pGEX-4T-1 and the recombinant human MGF (rhMGF) protein could be expressed in Rosetta (DE3) by isopropyl β-D-1-thiogalactopyranoside induction but not in BL21 (DE3). Mutation from rare codons to Escherichia coli preferred ones is performed. We obtain MGF(Mut54-56) and MGF(Mut-total) fragments through site-directed mutagenesis and overlapping PCR. Both pGEX-4T-1/MGF(Mut54-56)- and pGEX-4T-1/MGF(Mut-total)-transformed BL21 (DE3) can be induced to express rhMGF protein. To optimize the production technology, expression and purification of rhMGF are analyzed and compared in Rosetta (DE3) and BL21 (DE3). Results
Cited for: VARIANTS TRP-18; CYS-34; HIS-34; LEU-216; SER-286; SER-291; MET-299; CYS-376; GLY-447; ALA-449; VAL-461; SER-475; TRP-481; TYR-524; ARG-558; HIS-568; ARG-579; LYS-592; GLY-596; ALA-601; PHE-618; ASP-638; LEU-656; THR-672; HIS-689; LYS-692; PHE-705; ILE-924; GLN-986; MET-1016; ARG-1040; ALA-1082; LEU-1090; LEU-1098; TYR-1103; LYS-1107; TRP-1116; GLN-1193; MET-1251; SER-1293; PHE-1308; TRP-1512; ASN-1787; THR-1836; LYS-1901; CYS-1919; LEU-1951; GLN-1958; LEU-1962; MET-1968; GLN-1991; LEU-2004 AND ALA-2006; VARIANTS BRGDA1 GLN-18; LYS-70; ASN-84; SER-93; SER-94; GLN-104; TRP-104; LYS-109; GLN-121; TRP-121; GLU-126; PRO-136; MET-146; GLN-161; LYS-161; ASN-175; GLY-178; ARG-182; VAL-185; VAL-204; GLN-212; ILE-220; GLN-222; LEU-223; TRP-225; VAL-226; ILE-232; MET-240; LYS-270; GLN-276; ASP-278; CYS-282; ILE-300; PRO-315; ASN-320; ARG-325; LEU-336; ASP-351; VAL-351; ASN-356; CYS-367; HIS-367; LEU-367; LYS-369; GLY-374; HIS-376; ARG-386; GLU-386; ALA-396; LEU-396; LYS-439; GLY-501; HIS-526; ...
31150DNAArtificial Sequencesynthetic primer for the site-directed mutagenesis 1cctccccctg aacctgaaac aagatctgaa tgcaattgtt gttgttaacg 50251DNAArtificial Sequencesynthetic primer for the site-directed mutagenesis 2cgttaacaac aacaattgca ttcaggatct tgtttcaggt tcagggggag g 51316DNAArtificial Sequencesynthetic primer, RT-telo-4 3ctcatatatt cagtat 16415DNAArtificial Sequencesynthetic primer, RT-telo-3 4ctggacactc gctca 15515DNAArtificial Sequencesynthetic primer, RT-telo-2 5tcagccggac atgca 15615DNAArtificial Sequencesynthetic primer, RT-telo-1 6tcactcaggc ctcag 15717DNAArtificial Sequencesynthetic primer, RCR-telo-10 7gctggtgtct gctctcg 17825DNAArtificial Sequencesynthetic primer, RCR-telo-11 8ctgcagcagg aggatcttgt agatg 25921DNAArtificial Sequencesynthetic primer, RCR-telo-6 9gcaggtgaac agcctccaga c 211024DNAArtificial Sequencesynthetic primer, RCR-telo-R13 10cacaggctgc agagcagcgt ggag 241125DNAArtificial Sequencesynthetic primer, RCR-telo-F12 11gtcctacgtc cagtgccagg ggatc 251225DNAArtificial ...
For our British Journal of Pharmacology publication regarding the structural chemogenomics analysis of aminergic GPCRs (publication) we created a comprehensive overview of all site-directed mutagenesis studies performed against HRH1, HRH2, HRH3, HRH4, DRD3, ACM2, ACM3, ADRB1 and ADRB2 (aminergic GPCRs). In total 1420 reported single-point mutations were registered (including their effect on the binding affinity of reported ligands) at 128 individual B&W positions. ...
Cited for: VARIANTS EIEE6 PHE-17 DEL; THR-68; ASN-79; CYS-84; PRO-98; GLN-101; TRP-101; ARG-108; ASP-127; ARG-199; SER-227; THR-227; SER-232; ARG-233; VAL-342; ASP-343; TRP-351; SER-359; ARG-363; ARG-384; CYS-393; HIS-393; VAL-400; VAL-403; PHE-406; GLY-626; ASP-762; THR-785; ILE-812; ARG-842; 854-GLY-LEU-855 DEL; CYS-859; GLN-862; PRO-890; CYS-932; PRO-933; CYS-946; HIS-946; ARG-950; LYS-954; LYS-956; LEU-957; ILE-976; VAL-979; ARG-993; 999-ASN-LEU-1000 DELINS LEU-ILE-SER; LYS-1208; LYS-1221; PHE-1230; ASP-1238; ALA-1266; ASN-1288; VAL-1320; PRO-1326; GLY-1350; ARG-1358; PRO-1370; HIS-1378; THR-1378; ILE-1394; TYR-1396; SER-1417; PHE-1423; ALA-1429 DEL; VAL-1433; LYS-1450; SER-1451; LYS-1454; HIS-1462; LYS-1476; LYS-1503; GLY-1544; GLU-1586; ARG-1588; HIS-1592; PRO-1592; SER-1605; GLU-1637; THR-1638; CYS-1648; GLU-1653; PRO-1660; PRO-1667; LEU-1668; ILE-1672; THR-1673; THR-1683; ASP-1684; TRP-1688; ARG-1714; ASN-1763; ASN-1770; PHE-1770; THR-1770; THR-1780; VAL-1783; LYS-1787; PRO-1832; ...
Sodium, Electrophoresis, Escherichia, Escherichia Coli, Family, Flexibility, Inhibition, Muscle, Mutagenesis, PH, Polyacrylamide Gel Electrophoresis, Polymerization, Polymers, Report, Serpins, Site-directed Mutagenesis, Skeletal Muscle, Sodium Dodecyl Sulfate, Temperature
We have constructed highly constitutively active H3 receptors (MT6; A357K) by induced point mutagenesis in the C-terminal portion of the third intracellular loop of mouse H3 receptors. This substitution resulted in three major biochemical modifications of the H3 receptors: 1) increased constitutive activity leading to agonist-independent inhibition of adenylyl cyclase in cells; 2) moderately increased affinity for some agonists; and 3) reduced affinities for antagonist acting as inverse agonists.. Several mutagenesis studies indicated that the C-terminal portion of the i3 might act in concert with other structural domains to determine the selectivity and efficiency of G-protein-coupling receptor. For the adrenergic receptors, it has been shown that the C-terminal portion of the i3 plays a crucial role in G-protein-coupling receptor (ODowd et al., 1988; Cotecchia et al., 1990; Liggett et al., 1991). Ren et al. (1993) previously showed that the T373K mutation adjacent to TM6 causes dramatically ...
Mixed bases are used in primers to bind to templates that contain variability or a mixture of sequences at the primer binding sites. Mixed bases can also be used to create diversity in clone libraries and in site directed mutagenesis.. IDT offers random base oligos. Order them by using an upper case letter N or other IUPAC-IUB symbol (see table below). IDT offers two types of randomization, machine mix and hand mix. Machine mix bases are charged at the standard base price for the scale ordered. Hand mixing is done to provide custom ratios of the bases, and incurs an additional charge. When entering your sequence, the "Mixed Base" tab at the bottom of the page lists the IUB symbols and is where custom mix ratios need to be entered ...
Buy In Vitro Mutagenesis Protocols (Methods in Molecular Biology v. 57) by TROWER From WHSmith today! FREE delivery to store or FREE UK delivery on all ...
1ENA: Crystal structures of the binary Ca2+ and pdTp complexes and the ternary complex of the Asp21Glu mutant of staphylococcal nuclease. Implications for catalysis and ligand binding.
Noncysteinyl coordination to the [4Fe-4S]2+ cluster of the DNA repair adenine glycosylase MutY introduced via site-directed mutagenesis. Structural characterization of an unusual histidinyl-coordinated cluster ...
Dear Netters I am looking for the GUS gene from which the glycosylation sites have been removed by site directed mutagenesis. I know it is around but I dont have the reference. Sjef Smeekens Utrecht University email: wbtmcbw at cc.ruu.nl ...
Virtual plasmid consisting of wild-type human ubiquitin cloned between KpnI and NotI sites of pCDNA3.1(+)-Zeo. Used for site-directed mutagenesis tutorial.. 1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT ...
Production of ser125-interleukin-2 by site directed mutagenesis / Seongman Kang; Ju Won Kwak; Jong Bum Kwon; Sung Wan Kim; In Young Lee; Sun Bok Lee; Hye-Young Yun; Kyung Soo Hahm; Moon Hi Han; Doe Sun Na , 1989 ...
Mutagenesis of the mouse Bhlhb4 gene. A: Gene targeting strategy showing partial restriction map of WT Bhlhb4 allele, the targeting vector, the targeted ES cell
Melet, A., Marques-Soares, C., Schoch, G. A., Macherey, A. C., Jaouen, M., Dansette, P. M., Sari, M. A., Johnson, E. F., Mansuy, D. Analysis of human cytochrome P4502C8 substrate specificity using a substrate pharmacophore and site-directed mutants Biochemistry 2004 43:15379-15392 DOI:10.1021/bi0489309 PMID:15581350 ...
Despite the extensive work being performed to understand cancer and carcinogenic properties of chemicals and other agents, there are still gaps in our ability t...