Mucins are large glycoproteins that cover epithelial surfaces of the body. All mucins contain at least one PTS domain, a region rich in proline, threonine and serine. Mucins are also characterized by von Willebrand D (VWD) domains or SEA domains. We have developed computational methods to identify mucin genes and proteins based on these properties of the proteins. Using such methods we are able to characterize different organisms where genome sequence is available with respect to their mucin repertoire. We have here made a comprehensive analysis of potential mucins encoded by the chicken (Gallus gallus) genome. Three transmembrane mucins (Muc4, Muc13, and Muc16) and four gel-forming mucins (Muc6, Muc2, Muc5ac, and Muc5b) were identified. The gel-forming mucins are encoded within a locus similar to the corresponding human mucins. However, the chicken has an additional gene inserted between Muc2 and Muc5ac that encodes the the α-subunit of ovomucin, a protein similar to Muc2, but it is lacking a PTS
McGuckin M.A., Wright G. and Ward B.G. (1991) Expression of a polymorphic epithelial mucin antigen defined by the monoclonal antibody BC2 in ovarian carcinoma: Use of the BC2 antibody for the detection of micrometastases. American Journal of Clinical Pathology, 96 1: 46-52. ...
0005] The glycocalyx of the corneal epithelium contains the transmembrane mucin (glycosylated glycoproteins present in the glycocalyx), MUC1, MUC4, and MUC16. Mucins are high molecular weight Glycoproteins that provide a protective layer on corneal epithelial surfaces and are involved in cell-cell interactions, signaling, and metastasis. MUCIN 1, a cell surface associated (MUC 1) or polymorphic epithelial mucin (PEM) is a mucin, the protein part of which is encoded by the MUC1 gene in humans. Mucin proteins penetrate the membranes of epithelial cells, on the inner surface of the intestine and other organs. Mucin protects the body from infection by binding to pathogens. These membrane mucins interact with soluble, secreted, gel-forming mucin produced by the goblet cells (MUC5AC) and also with others like MUC2. The lacrimal gland also secretes MUC7 into the tear film. These soluble mucin in the tear film moves freely, (made possible by blinking and electrostatic repulsion from the negatively ...
The sustained delivery of both hydrophobic and hydrophilic drugs from hydrogels remains a challenge that requires the design of complex multifunctional synthetic polymers. In this work we exploit mucin glycoproteins, the gel-forming building blocks of natural mucus to deliver both hydrophobic and hydrophilic drugs. Mucins are a family of high molecular weight proteins which are densely glycosylated. Their central protein backbone contains hydrophobic and charged domains, while the mucin-associated glycans provide hydrogen bonding capabilities, high hydration, and negative charges. The biochemical versatility of mucins represents potential binding sites for certain drugs. If assembled into hydrogels, mucins could prevent drugs from freely diffusing out, leading to their sustained delivery. We generated methacrylated mucins which assembled into a covalently crosslinked mucin hydrogel when exposed to UV light. The rheological properties of the mucin gels were dominated by an elastic component, and ...
Goblet cells reside throughout the gastrointestinal (GI) tract and are responsible for the production and preservation of a protective mucus blanket by synthesizing and secreting high molecular weight glycoproteins known as mucins. The concept of the mucus layer functioning as a dynamic protective barrier is suggested by studies showing changes in mucins in inflammatory conditions of the GI tract, by the altered goblet cell response in germ-free animals, and by the enhanced mucus secretion seen in response to infections. The mucin-containing mucus layer coating the GI epithelium is the front line of innate host defense. Mucins are likely to be the first molecules that invading pathogens interact with at the cell surface and thus, can limit binding to other glycoproteins and neutralize the pathogen. This review will focus on what is known about goblet cell response in various GI infections and the regulatory networks that mediate goblet cell function and mucin production in response to intestinal insults
TY - JOUR. T1 - Single molecule study of heterotypic interactions between mucins possessing the Tn cancer antigen. AU - Haugstad, Kristin E.. AU - Stokke, Bjørn T.. AU - Brewer, Curtis F.. AU - Gerken, Thomas A.. AU - Sletmoen, Marit. PY - 2015. Y1 - 2015. N2 - Mucins are linear, heavily O-glycosylated proteins with physiological roles that include cell signaling, cell adhesion, inflammation, immune response and tumorgenesis. Cancer-associated mucins often differ from normal mucins by presenting truncated carbohydrate chains. Characterization of the binding properties of mucins with truncated carbohydrate side chains could thus prove relevant for understanding their role in cancer mechanisms such as metastasis and recognition by the immune system. In this work, heterotypic interactions of model mucins that possess the Tn (GalNA-cαThr/Ser) and T (Galβ1-3GalNAcαThr/Ser) cancer antigens derived from porcine submaxillary mucin (PSM) were studied using atomic force microscopy. PSM possessing only ...
Figure 1. Yearly number of publication related to mucins as reported by scicurve.com, using the keyword "mucins".. This is because mucin research spans over many fields. Biologists have been intrigued by mucins since long. But more recently, microbiologists studying our microbiome have new reasons to find this class of biopolymers particularly exciting. For instance, they realized that intestinal mucus plays key roles in the behavior of both pathogenic and commensal bacteria. This will surely lead to many more microbiology studies with mucus/mucins as a key component (1,2). Similarly, recent findings in immunology describing interactions between dendritic cells and mucin-associated glycans will also attract attention to secreted mucins in that field (3).. Some physicists and biophysicists are also fascinated by mucins. Tribologists are trying to understand and recreate mucins efficient lubricity that is so precious for a healthy mouth and gastrointestinal tract (4,5). The field of oral drug ...
The mucin-type glycoprotein podoplanin is specifically expressed by lymphatic but not blood vascular endothelial cells in culture and in tumor-associated lymphangiogenesis, and podoplanin deficiency results in congenital lymphedema and impaired lymphatic vascular patterning. indicated by granulosa cells in regular ovarian follicles highly, and by ovarian granulosa and dysgerminomas cell tumors. Although podoplanin was absent from regular human being epidermis mainly, its manifestation was induced in 22 of 28 squamous cell carcinomas studied strongly. These findings recommend a potential part of podoplanin in tumor development, plus they also determine the 1st commercially obtainable antibody for the precise staining of a precise lymphatic marker in archival human being tissue sections, allowing more widespread research of tumor lymphangiogenesis in human cancers thereby. Lymphatic vessels play a significant part in the maintenance of cells homeostasis1 and in the transportation of immune system ...
Mucins (/ˈmjuːsɪn/) are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by epithelial tissues in most animals.[1] Mucins key characteristic is their ability to form gels; therefore they are a key component in most gel-like secretions, serving functions from lubrication to cell signalling to forming chemical barriers.[1] They often take an inhibitory role.[1] Some mucins are associated with controlling mineralization, including nacre formation in mollusks,[2] calcification in echinoderms[3] and bone formation in vertebrates.[4] They bind to pathogens as part of the immune system. Overexpression of the mucin proteins, especially MUC1, is associated with many types of cancer.[5] Although some mucins are membrane-bound due to the presence of a hydrophobic membrane-spanning domain that favors retention in the plasma membrane, most mucins are secreted as principal components of mucus by mucous membranes or are secreted to become a component of saliva. ...
Liu, C., "Comparison of structural characteristics of major membrane-bound mucins from porcine milk and intestinal brush borders." (2003). Dairy Science Publication Database. 336 ...
Mucins are high-molecular weight glycoproteins (0.25-20 MDa) containing one or more domains that are heavily O-glycosylated. Their implications as targets for cancer treatment have increased the interest in these glycoproteins, mainly in the fields of vaccines and antibodies. However, mucins present high heterogeneity, posing challenges that affect purification processes and quality control analysis. In that sense, it is necessary to develop and improve downstream processes and analytical methods to characterize these products. Here a tool based on biolayer interferometry analysis to improve mucins detection and quantification in a fast, simple and label free-way is presented. Taking advantage of lectin recognition of mucins carbohydrate structures, several lectins were evaluated and immobilized on streptavidin biosensors. Different assay conditions were optimized and the most suitable lectin, Aleuria aurantia lectin (AAL), was selected. Bovine Submaxillary Gland and human MUC5B mucins were ...
TY - JOUR. T1 - Enhanced self-association of mucins possessing the T and Tn carbohydrate cancer antigens at the single-molecule level. AU - Haugstad, Kristin E.. AU - Gerken, Thomas A.. AU - Stokke, Bjørn T.. AU - Dam, Tarun K.. AU - Brewer, C. Fred. AU - Sletmoen, Marit. PY - 2012/5/14. Y1 - 2012/5/14. N2 - Mucins are linear O-glycosylated glycoproteins involved in inflammation, cell adhesion, and tumorigenesis. Cancer-associated mucins often possess increased expression of the T (Galβ1,3GalNAcαThr/Ser) and Tn (GalNAcαThr/Ser) cancer antigens, which are diagnostic markers for several cancers, including colon cancer. We have used AFM based single-molecule forced unbinding under near physiological conditions to investigate the self-interactions between porcine submaxillary mucin (PSM) as well as between PSM analogs possessing various carbohydrates including the T- and Tn-antigen. Distributions of unbinding forces and corresponding force loading rates were determined for force loading rates ...
We have previously reported the establishment of cytotoxic T-cell lines from pancreatic cancer patients, by continuously stimulating tumor-draining lymph node cells with allogeneic pancreatic tumor cell lines. After the preliminary characterization of their phenotype and tumor specificity, detailed studies performed with one of the cell lines, W.D., show that it recognizes a specific antigen, a large and heavily glycosylated mucin molecule, expressed on pancreatic and breast tumors and tumor cell lines. Although this recognition appears major histocompatibility complex (MHC)-unrestricted, the antigen receptor used by the cytotoxic T cell is the alpha/beta heterodimer, typically found on MHC-restricted T cells. The target antigen is atypical, however, in its ability to directly bind and activate the T cells in the absence of self MHC, presumably by abundant and regularly repeated antigenic epitopes. These findings are important because they demonstrate a specific T-cell response against a human ...
LAA413Hu71, Biotin-Linked Polyclonal Antibody to Mucin 1 (MUC1), Homo sapiens (Human), Labelled antibody, CD227, EMA, H23AG, KL-6, PEM, PEMT, PUM, CA15-3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Designed by Cloud-Clone Corp.
Pancreatic cancer is characterized by an often dramatic outcome (five year survival | 5%) related to a late diagnosis and a lack of efficient therapy. Therefore, clinicians desperately need new biomarkers and new therapeutic tools to develop new efficient therapies. Mucins belong to an ever increasing family of O-glycoproteins. Secreted mucins are the main component of mucus protecting the epithelia whereas membrane-bound mucins are thought to play important biological roles in cell-cell and cell-matrix interactions, in cell signaling and in modulating biological properties of cancer cells. In this review, we will focus on the altered expression pattern of mucins in pancreatic cancer, from the early neoplastic lesion Pancreatic Intraepithelial Neoplasia (PanIN) to invasive pancreatic carcinomas, and the molecular mechanisms (including genetic and epigenetic regulation) and signaling pathways known to control their expression. Moreover, we will discuss the recent advances about the biology of both
Mucins are high molar mass glycoproteins that assume an extended conformation and can assemble into mucus hydrogels that protect our mucosal epithelium. In nature, the challenging task of generating a mucus layer, several hundreds of micrometers in thickness, from micrometer-sized cells is elegantly solved by the condensation of mucins inside vesicles and their on-demand release from the cells where they suddenly expand to form the extracellular mucus hydrogel. We aimed to recreate and control the process of compaction for mucins, the first step toward a better understanding of the process and creating biomimetic in vivo delivery strategies of macromolecules. We found that by adding glycerol to the aqueous solvent, we could induce drastic condensation of purified mucin molecules, reducing their size by an order of magnitude down to tens of nanometers in diameter. The condensation effect of glycerol was fully reversible and could be further enhanced and partially stabilized by cationic ...
In gene-targeted mouse models for cystic fibrosis (CF), the disease is mainly manifested by mucus obstruction in the intestine. To explore the mucus composition, mucins insoluble and soluble in 6 M guanidinium chloride were purified by three rounds of isopycnic ultracentrifugation from the small and large intestines of CF mice (Cftr(m1UNC)/Cftr(m1UNC)) and compared with wild-type mice. The amino acid composition was typical of that for mucins and showed increased amounts of the insoluble (2.5-fold increase) and soluble (7-fold increase) mucins in the small intestine of the CF mice compared with wild-type mice. Mucins from the large intestine of both wild-type and CF mice showed a high but constant level of fucosylation. In contrast, the insoluble and soluble mucins of the small intestine in CF mice revealed a large increase in fucose, whereas those of wild-type mice contained only small amounts of fucose. This increased fucosylation was analysed by releasing the O-linked oligosaccharides ...
An unusual case of a mucin secreting benign microfollicular adenoma of the thyroid in a 30 year old euthyroid woman is reported. Histologically, the lesion was characterised by follicular cells with the appearance of signet ring cells. Histochemistry showed the mucin content of these cells to consist uniformly of sulphated acid mucins; positive thyroglobulin immunostaining was also shown. The published work on primary mucin secreting tumours of the thyroid gland is reviewed. Dual differentiation is thought to be responsible for combined mucin secretion and hormone production in this type of neoplasm.. ...
Efficient separation of mucins (200 kDa-2 MDa) was demonstrated using gradient SDS agarose/polyacrylamide composite gel electrophoresis (SDS-AgPAGE). Inclusion of urea (SDS-UAgPAGE) in the gels casting were shown to have no effect on the migration of mucins in the gel and allowed casting of gel at room temperature. This simplified the procedure for multiple casting of agarose polyacrylamide gradients and increased reproducibility of these gels. Hence, the implementation of urea makes the technique applicable for high throughput isolation and screening of mucin oligosaccharides by LC-MS after releasing the oligosaccharides from isolated, blotted mucin subpopulations. It was also shown that the urea addition had no effect on other supporting applications such as western and lectin blotting. In addition, identification of the mucin protein after tryptic digestion and LC-MS was possible and no protein carbamylation due to the presence of urea in the gel was detected. LC-MS software developed for ...
The selectin family of cell adhesion molecules mediates the tethering and rolling of leukocytes on blood vessel endothelium. It has been postulated that the molecular basis of this highly dynamic adhesion is the low affinity and rapid kinetics of selectin interactions. However, affinity and kinetic analyses of monomeric selectins binding their natural ligands have not previously been reported. Leukocyte selectin (L-selectin, CD62L) binds preferentially to O-linked carbohydrates present on a small number of mucin-like glycoproteins, such as glycosylation-dependent cell adhesion molecule-1 (GlyCAM-1), expressed in high endothelial venules. GlyCAM-1 is a soluble secreted protein which, following binding to CD62L, stimulates beta2-integrin-mediated adhesion of lymphocytes. Using surface plasmon resonance, we show that a soluble monomeric form of CD62L binds to purified immobilized GlyCAM-1 with a dissociation constant (Kd) of 108 microM. CD62L dissociates from GlyCAM-1 with a very fast dissociation rate
Latest reports claim that autoantibodies directed to glycosylated mucins aberrantly, specifically MUC4 and MUC1, are located in individuals with colorectal cancer. Rosetta2 (Novagen), nickel purified using NiNTA agarose (Qiagen, Venlo, NL) accompanied by HPLC purification before and after O-glycosylation. Items were analyzed by MALDI-TOF and SDS-PAGE mass spectrometry on the Voyager-DE? PRO workstation (Applied Biosystems, Lifestyle Technology, Carlsbad, CA) using 2,5-dihydroxybenzoic acidity (Sigma, St. Louis, Missouri) as matrix. AB1010 Microarray MUC1, MUC4 and MUC2 glycopeptides and glycoproteins were printed on Schott Nexterion? Glide H MPX 48 (Schott AG, Mainz, Germany) as previously defined.18 In brief, triplicates of most peptides, in 150 mM sodium phosphate pH 8.5 with 0.005% CHAPS, were printed on the BioRobotics MicroGrid II spotter (Genomics Solution) having a 0.21-mm pitch using Stealth 3B Micro Spotting Pins (Telechem International ArrayIt Division). After printing, slides had been ...
TY - JOUR. T1 - Mechanistic and functional shades of mucins and associated glycans in colon cancer. AU - Pothuraju, Ramesh. AU - Krishn, Shiv Ram. AU - Gautam, Shailendra K.. AU - Pai, Priya. AU - Ganguly, Koelina. AU - Chaudhary, Sanjib. AU - Rachagani, Satyanarayana. AU - Kaur, Sukhwinder. AU - Batra, Surinder K.. PY - 2020/3. Y1 - 2020/3. N2 - Mucus serves as the chief protective barrier against pathogenic and mechanical insults in respiratory, gastrointestinal, and urogenital tracts. Altered mucin expression, the major component of mucus, in conjunction with differential glycosylation has been strongly associated with both benign and malignant pathologies of colon. Mucins and their associated glycans arbitrate their impact sterically as well as mechanically by altering molecular and microbial spectrum during pathogenesis. Mucin expression in normal and pathological conditions is regulated by nonspecific (dietary factors and gut microbiota) and specific (epigenetic and transcriptional) ...
To investigate the genetic identities of the mucins secreted in cystic fibrosis (CF) airways, sputum was collected from five individuals. Samples were separated into gel and sol phases by high-speed centrifugation and the gel phase was extracted in 6 M guanidinium chloride. The insoluble residue remaining after extraction of the gel phase was brought into solution by reduction/alkylation. Density-gradient centrifugation in CsCl revealed polydisperse distributions of sialic acid-containing mucins in the gel phase, insoluble residue and sol phase fractions and the degree of variation between the different individuals was low. Antibodies recognizing MUC5AC and MUC5B identified these mucins in each of the fractions. MUC2, however, was present only as a component of the insoluble residue from the gel which accounted for less than 4% by mass of the total mucins. MUC5B and MUC5AC from the gel phase were large oligomeric species composed of disulphide-bond linked subunits and MUC5B was present as two ...
Mucins are the primary macromolecular component of mucus-natures natural lubricant-although they are poorly characterised heterogeneous substances. Recent advances in hydrodynamic methodology now offer the opportunity for gaining a better understanding of their solution properties. In this study a combination of such methods was used to provide increased understanding of a preparation of porcine intestinal mucin (PIM), MUC2 mucin, in terms of both heterogeneity and quantification of conformational flexibility. The new sedimentation equilibrium algorithm SEDFIT-MSTAR is applied to yield a weight average (over the whole distribution) molar mass of 7.1 × 106 g mol−1, in complete agreement with size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS), which yielded a value of 7.2 × 106 g mol−1. Sedimentation velocity profiles show mucin to be very polydisperse, with a broad molar mass distribution obtained using the Extended Fujita algorithm, consistent with the ...
Looking for mucin clot test? Find out information about mucin clot test. see glycoprotein glycoprotein , organic compound composed of both a protein and a carbohydrate joined together in covalent chemical linkage. These... Explanation of mucin clot test
From repairing skin damage and providing anti-aging benefits to soothing irritated skin and fading acne scars, snail mucin, or snail secretion filtrate, offers so many benefits. Its truly a versatile ingredient, suitable for all skin types and concerns, and plays well with other ingredients. Studies have shown that snail mucin even helps with atopic dermatitis. In fact, even before snail mucin became popular as a skin care ingredient, the main component of snail mucin, Chondroiton sulfate, was recognized for its ability to help improve arthritis, liver diseases, nephritis, and fatigue.. But have you ever wondered where snail mucin comes from? You might be thinking that snails are plucked from their natural habitats and forced to produce mucin, which eventually ends up in pretty packaging on store shelves. Or you might have assumed that snail mucin is artificially created in a petri dish and then mass produced to meet the high demand for snail mucin. The answer is none of the above. Read on for ...
... , Authors: Raquel Mejías-Luque, Lara Cobler, Carme de Bolós. Published in: Atlas Genet Cytogenet Oncol Haematol.
TY - JOUR. T1 - Mucins in pancreatic cancer and its microenvironment. AU - Kaur, Sukwinder. AU - Kumar, Sushil. AU - Momi, Navneet. AU - Sasson, Aaron R.. AU - Batra, Surinder Kumar. PY - 2013/10/1. Y1 - 2013/10/1. N2 - Pancreatic cancer remains a lethal malignancy with poor prognosis owing to therapeutic resistance, frequent recurrence and the absence of treatment strategies that specifically target the tumour and its supporting stroma. Deregulated cell-surface proteins drive neoplastic transformations and are envisioned to mediate crosstalk between the tumour and its microenvironment. Emerging studies have elaborated on the role of mucins in diverse biological functions, including enhanced tumorigenicity, invasiveness, metastasis and drug resistance through their characteristic O-linked and N-linked oligosaccharides (glycans), extended structures and unique domains. Multiple mucin domains differentially interact and regulate different components of the tumour microenvironment. This Review ...
Mucin 1 (MUC1) is a heterodimeric protein that is aberrantly expressed by diverse human carcinomas and certain hematological malignancies (Kufe, 2009). The overexpression of MUC1, as found in human cancers, is associated with the induction of anchorage-independent growth and tumorigenicity (Li et al., 2003). Based on these findings, MUC1 has emerged as an attractive target for the development of anticancer agents. However, the identification of drugs that block MUC1 has been limited by the lack of sufficient information regarding how MUC1 contributes to the growth and survival of malignant cells. In this regard, the MUC1 protein is translated by a single mRNA and then undergoes autocleavage into two subunits that in turn form a heterodimer (Kufe, 2009). The MUC1 N-terminal subunit is the mucin component of the heterodimer that contains the characteristic glycosylated tandem repeats and is expressed on the cell surface in a complex with the MUC1 C-terminal transmembrane subunit (MUC1-C) (Kufe, ...
TFF-2, human recombinant protein, Trefoil Factor 2, Spasmolytic polypeptide (SP), Spasmolysin validated in (PBV10395r-100), Abgent
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Natural fragmentation of polypeptide chains by autoproteolysis occurs in a number of protein families. It is a vital step in the maturation of several enzymes and in the formation of membrane-associated mucins that constitute a part of the protective mucus barrier lining epithelial cells. These reactions follow similar routes involving an initial N-O or N-S acyl shift starting with a nucleophilic attack by a hydroxyl or thiol group on a carbonyl carbon followed by resolution of the ester intermediate. Previous studies indicate that distortion of the scissile peptide bond may play a role in autoproteolysis. Our structural, biochemical and molecular dynamics studies of the autoproteolyzed SEA domains from human membrane-bound mucin MUC1 and human orphan receptor GPR116 confirmed this by revealing a novel biochemical mechanism where the folding free energy accelerates cleavage by imposing conformational strain in the precursor structure. This mechanism may well be general for autoproteolysis. The ...
臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。. To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of "NTU Repository" with "Academic Hub" to form NTU Scholars.. ...
Extraction, characterization, and use of mucin biopolymers for biomedical applications.. For thousands of years, and still today, humans have exploited natural materials for their benefit. For instance, wood-extracted cellulose fibers are used to make paper and animal-extracted hyaluronic acid is used in cosmetic anti wrinkle creams. In this proposal we will develop the use of mucins, i.e. a molecules extracted from mucus, for biomedical applications. Mucins are the main components of mucus, the gel that covers the wet epithelium of all mammals. Commonly secreted by animal tissues, they can be extracted from abundant sources such as pig stomachs and bovine submaxillary glands.. The study and technological use of mucins are hampered by the poor control over the quality of the mucins available. In this proposal, we first aim to develop a quality-control benchmark by measuring a series of physical and biochemical properties. Using this benchmark, we will then identify new processes to efficiently ...
TY - JOUR. T1 - Trefoil factor(s) and CA19.9. T2 - A promising panel for early detection of pancreatic cancer. AU - Jahan, Rahat. AU - Ganguly, Koelina. AU - Smith, Lynette M.. AU - Atri, Pranita. AU - Carmicheal, Joseph. AU - Sheinin, Yuri. AU - Rachagani, Satyanarayana. AU - Natarajan, Gopalakrishnan. AU - Brand, Randall E.. AU - Macha, Muzafar A.. AU - Grandgenett, Paul M.. AU - Kaur, Sukhwinder. AU - Batra, Surinder K.. PY - 2019/4. Y1 - 2019/4. N2 - Background: Trefoil factors (TFF1, TFF2, and TFF3) are small secretory molecules that recently have gained significant attention in multiple studies as an integral component of pancreatic cancer (PC) subtype-specific gene signature. Here, we comprehensively investigated the diagnostic potential of all the member of trefoil family, i.e., TFF1, TFF2, and TFF3 in combination with CA19.9 for detection of PC. Methods: Trefoil factors (TFFs) gene expression was analyzed in publicly available cancer genome datasets, followed by assessment of their ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Introduction: Wood smoke (WS) exposure is associated with increased prevalence of chronic bronchitis and rapid decline in lung function. However, potency of WS and cigarette smoke (CS) in activating mucin gene expression has not been compared.. Aims and objectives: We tested the hypothesis that WS is more potent than CS in causing mucous cell metaplasia (MCM).. Methods: C57BL/6 mice were exposed for 30 d to filtered air (FA) or CS for 5 days/week and 6 hours/day at 250 mg/m3 total particulate matter (TPM) or to WS for 2 h/d at 10 mg TPM/m3. Extent of MCM was determined in stained lung sections. Mucin gene expression was analyzed by Western blot, qRT-PCR, and immunofluorescent staining. Findings were replicated in differentiated primary airway epithelial cultures from human (HAEC) and murine cells (MAECs).. Results: WS exposure at 25-fold lower TPM concentrations than CS increased Muc5ac, Muc5b, SPDEF and Bcl-2 mRNAs in the lungs of mice, while these mRNAs were not significantly increased by FA ...
Beatson, Richard, Tajadura-Ortega, Virginia, Achkova, Daniela, Picco, Gianfranco, Tsourouktsoglou, Theodora-Dorita, Klausing, Sandra, Hillier, Matthew, Maher, John, Noll, Thomas, Crocker, Paul R, Taylor-Papadimitriou, Joyce, and Burchell, Joy M. "The mucin MUC1 modulates the tumor immunological microenvironment through engagement of the lectin Siglec-9". Nature Immunology 17.11 (2016): 1273-1281 ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Highly glycosylated, polymeric, secreted mucins such asMUC2 serve to coat and protect the gastrointestinal, reproductive, and respiratory tracts. Given their role in protection, large size, and the complex processes required for their biosynthesis, it is not surprising that alterations in mucins, and their levels of expression, are associated with several diseases. In certain cancers, mucin oligosaccharides are altered in several ways, including a reduction in number, shortening, the exposure of normally cryptic inner chain residues, and the expression of novel carbohydrate structures (21, 23). The levels and types of mucins expressed are altered in cancers as well. For example, human cancers, especially colon cancers, differ widely in the levels ofMUC2 they express (18). Most colon cancers express only low levels ofMUC2 compared with normal colon, but a class known as mucinous colon cancers expresses high levels ofMUC2, suggesting that they follow a different mutational pathway to malignancy ...
CAN-003 was a randomized, open-label, Phase 2 trial evaluating the safety, efficacy and immune outcomes of CVac, a mucin 1 targeted-dendritic cell (DC) treatment as a maintenance therapy to patients with epithelial ovarian cancer (EOC). Patients (n = 56) in first (CR1) or second clinical remission (CR2) were randomized (1:1) to standard of care (SOC) observation or CVac maintenance treatment. Ten doses were administered over 56 weeks. Both groups were followed for progression-free survival (PFS) and overall survival (OS). Fifty-six patients were randomized: 27 to SOC and 29 to CVac. Therapy was safe with only seven patients with Grade 3-4 treatment-emergent adverse events. A variable but measurable mucin 1 T cell-specific response was induced in all CVac-treated and some standard of care (SOC) patients. Progression free survival (PFS) was not significantly longer in the treated group compared to SOC group (13 vs. 9 months, p = 0.36, hazard ratio [HR] = 0.73). Analysis by remission status showed in the
A critical function of mucin in saliva is to protect the mouth from drying out. Mucin also lubricates the tissues and teeth in the mouth and protects them against pathogens and...
Cosrx Advanced Snail 96 Mucin Power Essence is a powerful anti-ageing cream with 96% Mucin. This skin-friendly snail extract repairs dry and damaged skin cells.
1. Thim L. A new family of growth factor-like peptides. Trefoil disulphide loop structures as a common feature in breast cancer associated peptide (pS2), pancreatic spasmolytic polypeptide (PSP), and frog skin peptides (spasmolysins). FEBS Lett. 1989;250:85-90 doi:0014-5793(89)80690-8 2. Thim L, May FE. Structure of mammalian trefoil factors and functional insights. Cell Mol Life Sci. 2005;62:2956-73 doi:10.1007/s00018-005-5484-6 3. Baker ME. Oestrogen-induced pS2 protein is similar to pancreatic spasmolytic polypeptide and the kringle domain. Biochem J. 1988;253:307-9 4. Rio MC, Bellocq JP, Daniel JY, Tomasetto C, Lathe R, Chenard MP. et al. Breast cancer-associated pS2 protein: synthesis and secretion by normal stomach mucosa. Science. 1988;241:705-8 5. Marchbank T, Westley BR, May FE, Calnan DP, Playford RJ. Dimerization of human pS2 (TFF1) plays a key role in its protective/healing effects. J Pathol. 1998;185:153-8 doi:10.1002/(SICI)1096-9896(199806)185:2,153::AID-PATH87,3.0.CO;2-5 6. ...
May 5, 2012. "Researchers at Massachusetts Institute of Technology studied the antiviral action of mucus and, in particular, the mucin molecules - long, threadlike proteins that are the main building blocks of mucus.". Go to News Coverage. ...
The Herbert lab is investigating how mucosal epithelia and macrophages function together to maintain and restore pulmonary function under steady-state and infectious conditions. Our main project centers upon Trefoil factor proteins, a largely enigmatic family of mucosal cytokines, in controlling immunity at the mucosal interface. Recent evidence shows that TFF proteins regulate members of the Wnt family of glycoproteins. Given the central role of Wnts in epithelial differentiation and regeneration, our work brings a clearer understanding of how Trefoil factor signaling controls barrier integrity through modulation of Wnt signaling. One of our most recent findings is the demonstration that TFF2 is expressed within various macrophage populations of humans and mice and promotes a wound healing/M2 phenotype. Our generation of a novel co-culture system called the macrophage-epithelial repair assay (MERA), which uses primary tracheal epithelia and tissue macrophages has facilitated the discovery ...
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Porcine pancreatic spasmolytic polypeptide (PSP) belongs to a large family of homologous growth factor-like polypeptides characterized by a disulfide-linked "trefoil motif," duplicated and conserved in various family members. PSP contains two trefoil motifs, has several pharmacological actions on the gut, and has growth factor properties on epithelial cells in vitro. The human PSP analogue, human spasmolytic polypeptide, appears to be involved in many regenerative situations and, especially, in healing gastrointestinal ulcers. One member of the trefoil family, pS2, is secreted in approximately 50% of estrogen-dependent human breast carcinomas, which has led to its use as a tumor prognostic marker. Both pS2 and human spasmolytic polypeptide are also widely expressed in chronic gastrointestinal ulcerative conditions such as Crohn disease. Here we report the three-dimensional structure at 2.6-A resolution of a trefoil-containing protein, namely PSP, purified from porcine pancreas. The structure ...