TY - JOUR. T1 - Chromosomal Localization of a Human Mucin Gene (MUC8) and Cloning of the cDNA Corresponding to the Carboxy Terminus. AU - Shankar, Viswanathan. AU - Pichan, Prabhasankar. AU - Eddy, Roger L.. AU - Tonk, Vijay. AU - Nowak, Norma. AU - Sait, Sheila N J. AU - Shows, Thomas B.. AU - Schultz, Roger E.. AU - Gotway, Garrett. AU - Elkins, Ronald C.. AU - Gilmore, Michael S.. AU - Sachdev, Goverdhan P.. PY - 1997. Y1 - 1997. N2 - A partial cDNA (pAM1) encoding a major airway mucin glycoprotein with novel tandem repetitive sequence has recently been cloned. In this article, we report additional new sequence derived by 3′-rapid amplification of cDNA ends technique. The sequence corresponds to a stop codon, 3′-untranslated region of 458 bp, a polyadenylation signal, and poly A+ tail, and represents the extreme carboxy terminus of MUC8. A plasmid construct (pAM3) in pBluescript was generated by in-frame ligation of pAM1 to the 479-bp 3′UTR of MUC8. A 5′-end 325-bp fragment of this ...
Background Severe asthma is a frequent cause of hospital admission, especially among children. The main environmental triggers of airway inflammation in asthma are viruses and aeroallergens. These agents elicit reciprocal immune responses, characterised by production of T helper 1 and T helper 2 cytokines, respectively. There is no genetic explanation for how hyper-responsiveness to these disparate environmental stimuli develops among individuals with asthma. Our aim was to assess relation between an IL12B promoter polymorphism and asthma.. ...
TY - JOUR. T1 - Satiety and energy intake after single and repeated exposure to gel-forming dietary fiber: post-ingestive effects. AU - Wanders, A.J.. AU - Mars, M.. AU - Borgonjen-van den Berg, K.J.. AU - de Graaf, C.. AU - Feskens, E.J.M.. PY - 2014. Y1 - 2014. N2 - Background: Viscous or gel-forming dietary fibers can increase satiety by a more firm texture and increased eating time. Effects of viscous or gel-forming fibers on satiety by post-ingestive mechanisms such as gastric emptying, hormonal signals, nutrient absorption or fermentation are unclear. Moreover, it is unclear whether the effects persist after repeated exposure. Objective: To investigate satiety and energy intake after single and repeated exposure to gelled fiber by post-ingestive mechanisms. Design: In a two-arm crossover design, 32 subjects (24 female subjects, 21±2 y, BMI 21.8±1.9¿kg¿m-2) consumed test foods once daily for 15 consecutive days, with 2 weeks of washout. Test foods were isocaloric (0.5¿MJ, 200¿g) with ...
The present study was undertaken to analyze the role of the MUC4 mucin in pancreatic tumor development and progression. Mucins are generally known to protect surface epithelia from injuries and infections under normal physiological conditions but often display deregulated expression in malignant cells (4 , 6 , 10) . We have previously reported that the human mucin gene MUC4 is aberrantly expressed in pancreatic tumors and cell lines but has no detectable expression in the normal pancreas (10) . The present in vitro and in vivo data reveal that the silencing of MUC4 expression results in altered tumor cell behavior, decreased growth, and a marked reduction in metastatic incidences in an orthotopic mouse model of pancreatic cancer. These results are in support of the previous observations and establish a direct association of MUC4 expression with the disease development.. Down-regulating the expression is one of the convenient and reliable approaches for functional analyses of genes (reviewed in ...
CA125 is a high molecular weight mucin type glycoprotein, originally defined by the Oc125 monoclonal antibody (MAb) established by Bast et al. (1). Different epitopes, co-expressed with the Oc125 epitope on the CA125 antigen, have been used for the development of heterologous assays for determination of the CA125 antigen (2). The CanAg CA125 EIA is based on two mouse monoclonal antibodies, Ov197 and Ov185, directed against two independent epitopes of the protein core of the CA125 antigen (3, 4). Assays for CA125 are frequently used to monitor patients with gynecological malignancies such as epithelial ovarian cancer (5). ...
In adults, the epitope is typically expressed in trace amounts on mucosal cells of gastrointestinal epithelia. In patients with malignant disease, the epitope may appear associated with high molecular weight mucin in blood. Assays for CA19-9 are frequently used to monitor gastrointestinal malignancies such as pancreatic, gall bladder, gastric, and colorectal cancers (2, 3). ...
Adsay NV, Merati K, Nassar H, Shia J, Sarkar F, Pierson CR, Cheng JD, Visscher DW, Hruban RH, Klimstra DS. Pathogenesis of colloid (pure mucinous) carcinoma of exocrine organs: Coupling of gel-forming mucin (MUC2) production with altered cell polarity and abnormal cell-stroma interaction may be the key factor in the morphogenesis and indolent behavior of colloid carcinoma in the breast and pancreas. Am J Surg Pathol. 2003 May;27(5):571-8. abstract ...
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Mucins are large glycoproteins that cover epithelial surfaces of the body. All mucins contain at least one PTS domain, a region rich in proline, threonine and serine. Mucins are also characterized by von Willebrand D (VWD) domains or SEA domains. We have developed computational methods to identify mucin genes and proteins based on these properties of the proteins. Using such methods we are able to characterize different organisms where genome sequence is available with respect to their mucin repertoire. We have here made a comprehensive analysis of potential mucins encoded by the chicken (Gallus gallus) genome. Three transmembrane mucins (Muc4, Muc13, and Muc16) and four gel-forming mucins (Muc6, Muc2, Muc5ac, and Muc5b) were identified. The gel-forming mucins are encoded within a locus similar to the corresponding human mucins. However, the chicken has an additional gene inserted between Muc2 and Muc5ac that encodes the the α-subunit of ovomucin, a protein similar to Muc2, but it is lacking a PTS
Patented Nov. 1950 MODI FIED GEL-FORMING CLAY AND PROCESS OF PRODUCING SAME ErnstA. Hauser, Cambridge, Mass. No Drawing. Application May 3, 1946, Serial No. 667,119 This invention relates to a modified gel-forming clay and a process of making the same. The gel-forming clay is one which is dispersible in an organic liquid to form a gel therein, as distinguished from a clay which is dispersible in water to form a gel therein. Although certain colloidal clays, chiefly bentonites, when placed in water will swell to many times their dry volumes and in so doing will form gels or pastes of high consistency useful either without further treatment or in mixture with other materials to form in some cases emulsions. gels embodying this invention may be obtained in liquids other than water. The aforementioned gels of bentonite in water are subject to dryin shrinking, and cracking by reason of evaporation of the liquid phase, and similarly, emulsions of organic liquids or solids in aqueous bentonite gels ...
A method for controlling color bleed between adjacent multi-color ink regions on a print medium is disclosed. Color bleed involves the migration of color agents between adjacent zones in a multicolored printed image on a print medium. A first composition containing a gel-forming species and a color agent is brought into contact on a region of the print medium with a second composition having a color agent and a gel-initiating species or chemical conditions which bring about gelation. In alternative embodiments, the print medium may be pretreated with either a gel-initiating species or a gel-forming species (with no colorant), followed by treatment with a gel-forming species or gel-initiating species (with colorant), respectively. The formation of the gel upon the print medium impedes the movement of the color agent or agents and thus reduces the color bleed between adjacent zones.
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- The epithelial cell line, LS123, was established by L.P. Rutzky et al, in 1974 from the second in a series of three primary colonic tumors resected from a 65 year old Caucasian female.  In 1950, a primary colon tumor was resected from the patient (41 years old at that time). 24 years later a second primary tumor classified as a Dukes type B mucin producing colon adenocarcinoma was excised and the cells were cultured. A third primary tumor was discovered in 1980 in an area just proximal to the colostomy and was resected.
The level of Mucin 5B in unstimulated saliva was significantly lower in OLP patients. The level of Mucin 5B in stimulated saliva was also slightly, but not significantly, lower in OLP patients compared to healthy controls in our study. The level of Mucin 5B was higher in the serum of OLP patients. These findings may be explained as follows: an increase in the permeability of the salivary duct-blood barrier due to free oxygen radicals and other inflammatory mediators may occur in OLP patients (similar to respiratory distress syndrome), which results in an increase in serum level of Mucin 5B. On the other hand, some proteases play a role in conversion of high molecular weight mucin to low molecular weight mucin. Change in function of these proteases may occur due to inflammation in OLP and their activity may increase as such. As a result, mucin can more easily pass through the damaged salivary duct-blood barrier, compared to healthy individuals. Since this finding was seen for the first time in ...
... , Authors: Raquel Mejías-Luque, Lara Cobler, Carme de Bolós. Published in: Atlas Genet Cytogenet Oncol Haematol.
Evans CM, Raclawska DS, Ttofali F, Liptzin DR, Fletcher AA, Harper DN, McGing MA, McElwee MM, Williams OW, Sanchez E, Roy MG, Kindrachuk KN, Wynn TA, Eltzschig HK, Blackburn MR, Tuvim MJ, Janssen WJ, Schwartz DA, Dickey BF. The polymeric mucin Muc5ac is required for allergic airway hyperreactivity. Nat Commun. 2015 Feb 17; 6:6281 ...
臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。. To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of "NTU Repository" with "Academic Hub" to form NTU Scholars.. ...
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Complete information for MUC5AC gene (Protein Coding), Mucin 5AC, Oligomeric Mucus/Gel-Forming, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
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AGO study - adjuvant treatment, with NSAID, of endocrine ophthalmopathy in Graves´ disease. Background - Already at diagnosis of Graves disease approximately 98% of the patients have morphological changes of the retrobulbar tissue concordant with ophthalmopathy. Factors known to induce clinical symptoms of ophthalmopathy are mainly unknown. An interesting observation is that a patient with stable and inactive Graves´ disease developed ophthalmopathy when treated with a glitazone due to diabetes type 2. Glitazones have been shown to increase differentiation of orbital preadipocytes to mature adipocytes. Glitazones are PPAR-gamma agonists and recently diclofenac have been shown to interact with PPAR-gamma in physiological concentrations. Other non-steroidal antiinflammatory drugs, NSAID, like indomethacin lack this effect. In addition, diclofenac inhibit synthesis of prostaglandins which also may be of importance because the natural ligand to PPAR-gamma is prostaglandin J. Inflammation and ...
TY - JOUR. T1 - Up-regulation of MUC5AC and MUC5B mucin genes in chronic rhinosinusitis. AU - Kim, Dae Hoon. AU - Chu, Ho Suk. AU - Lee, Jin Young. AU - Hwang, Soon Jae. AU - Lee, Sang Hag. AU - Lee, Heung Man. PY - 2004/6/1. Y1 - 2004/6/1. N2 - Backgrounds: Excess mucus production and hypersecretion characterize upper airway diseases. The primary mechanisms leading to mucus hypersecretion in chronic rhinosinus inflammation are not well understood. Mucus hypersecretion is commonly accompanied by goblet cell and submucosal gland cell hyperplasia. It is important to identify which mucin gene messenger RNAs (mRNAs) are expressed in the sinus mucosa. Objectives: To investigate the expression of MUC5AC and MUC5B mRNAs and localization of these proteins in human sinus mucosa and to compare the expression of MUC5AC and MUC5B mRNAs in normal and in chronic sinus mucosa. Design: Twenty chronic maxillary sinusitis mucosa samples and 20 normal maxillary sinus mucosa samples were obtained; RNAs were ...
The transportability of the mucus layer on airway surfaces by the cilia is essential for maintaining normal lung health. This mucociliary clearance function of the airway epithelia is compromised in some diseases, notably CF, in which the mucus accumulates in and obstructs the airways, resulting in microbiological colonization. The pathogenic mechanism of this mucus obstruction is incompletely understood. The results of the present study provide evidence that an abnormal fluid environment on the airway surfaces corrupts the normal unfolding process of the MUC5B mucin glycoprotein, leading to the secretion of a dense, compact form of MUC5B that likely impairs its clearance by cilia.. The biophysical properties of the airway mucus layer are strongly influenced by qualitative and quantitative changes in mucins, the dominant macromolecules in airway mucus. Our group has previously shown that mucus secretions from CF lungs contain hyperconcentrated mucins that alter the osmotic pressure dynamics ...
Abstract: : Purpose: Our previous investigations proved that conjunctival impression cytology (CIC) is a valid, non-invasive technique that is useful to detect mRNA levels of ocular mucin genes in healthy individuals. This study was designed to perform the relative quantitation of MUC1, 2, 4, 5AC and 7 mucin genes in CIC samples from healthy males and females, younger and older of 60 years of age. Methods: Two polyethersulfone filters were applied to the superior bulbar conjunctiva of 38 healthy donors. Two age categories were considered: less that 60 years (7 males, 7 females), and 60 years or older (15 males, 9 females). Real time RT-PCR was performed using total RNA to examine the expression of MUC1, 2, 4, 5AC and 7. Their relative quantity was determined by SYBR® Green I dye detection. The housekeeping gene GAPDH was used to normalize the amount of expression levels. Data of mucin genes were analyzed using a logarithmic transformation to obtain a distribution closer to the normal ...
Mucin glycoproteins play a key role in the normal function of the airway epithelium. We examined the expression of mucin genes, MUC3, 4, 5AC, 5B, 6, 7, and 8 in human fetal tissues to establish the localization and age of onset of expression of each mucin gene during human development. We detected expression of MUC4, 5AC, 5B, and 7 in the mid-trimester airway epithelium but did not detect expression of MUC3, 6, or 8. MUC4 was expressed in the trachea and large airways in the majority of cells in the airway epithelium. Expression of MUC5AC was only seen in individual goblet cells in the trachea, while MUC5B was expressed in the surface epithelium of the trachea at 13 wk but was largely restricted to submucosal glands by 23 wk of gestation.
Introduction: Wood smoke (WS) exposure is associated with increased prevalence of chronic bronchitis and rapid decline in lung function. However, potency of WS and cigarette smoke (CS) in activating mucin gene expression has not been compared.. Aims and objectives: We tested the hypothesis that WS is more potent than CS in causing mucous cell metaplasia (MCM).. Methods: C57BL/6 mice were exposed for 30 d to filtered air (FA) or CS for 5 days/week and 6 hours/day at 250 mg/m3 total particulate matter (TPM) or to WS for 2 h/d at 10 mg TPM/m3. Extent of MCM was determined in stained lung sections. Mucin gene expression was analyzed by Western blot, qRT-PCR, and immunofluorescent staining. Findings were replicated in differentiated primary airway epithelial cultures from human (HAEC) and murine cells (MAECs).. Results: WS exposure at 25-fold lower TPM concentrations than CS increased Muc5ac, Muc5b, SPDEF and Bcl-2 mRNAs in the lungs of mice, while these mRNAs were not significantly increased by FA ...
In the present study, we investigated whether tussilagone, a natural product derived from Tussilago farfara , significantly affects the production and gene expression of airway MUC5AC mucin. Confluent NCI-H292 cells were pretreated with tussilagone for 30 min and then stimulated with EGF (epidermal growth factor) or PMA (phorbol 12-myristate 13-acetate) for 24 h or the indicated periods. The MUC5AC mucin gene expression was measured by RT-PCR. Production of MUC5AC mucin protein was measured by ELISA. To elucidate the action mechanism of tussilagone, effect of tussilagone on PMA-induced NF-κB signaling pathway was investigated by western blot analysis ...
The magnetic microrheometer technique was used to measure the rheologic properties of microliter quantities of mucus. For these measurements, a 70- to 150-p.m steel ball is positioned in the sample of mucus and oscillated by means of an electromagnet at different driving frequencies. The magnitude of displacement of the sphere…. ...
Author(s): Ren, Binhui; Azzegagh, Zoulikha; Jaramillo, Ana M; Zhu, Yunxiang; Pardo-Saganta, Ana; Bagirzadeh, Rustam; Flores, Jose R; Han, Wei; Tang, Yong-Jun; Tu, Jing; Alanis, Denise M; Evans, Christopher M; Guindani, Michele; Roche, Paul A; Rajagopal, Jayaraj; Chen, Jichao; Davis, C William; Tuvim, Michael J; Dickey, Burton F | Abstract: Airway mucin secretion is important pathophysiologically and as a model of polarized epithelial regulated exocytosis. We find the trafficking protein, SNAP23, selectively expressed in secretory cells compared to ciliated and basal cells of airway epithelium by immunohistochemistry and fluorescence activated cell sorting, suggesting that SNAP23 functions in regulated but not constitutive epithelial secretion. Heterozygous SNAP23 deletant mutant mice show spontaneous accumulation of intracellular mucin, indicating a defect in baseline secretion. However mucins are release from perfused tracheas of mutant and wild-type mice at the same rate, suggesting that increased
Flour improvers, chlorinated flour replacers comprising the flour improver, methods of preparing the flour improvers and chlorinated flour replacers, and uses thereof (e.g. for improving the textural properties of wheat flour-based high ratio cakes) are provided. The flour improver as disclosed herein comprises between 10% and 97.5% (w/w) of non-wheat flour, wherein the non-wheat flour is one or more non-wheat flours selected from the group consisting of rice flour, oat flour, quinoa flour and buckwheat flour; between 2% and 20% (w/w) of a gel-forming protein in addition to protein present in the non-wheat flour; and between 0.5% and 3% (w/w) of calcium 2+ ions.
Objectives To identify the mucin gene and its expressing cells in the middle ear mucosa with chronic otitis media (COM), and to study the correlation between infiltration of inflammatory cells in the submucosa and expression of the mucin gene in the mucosal epithelium with COM.. Study Design Middle ear mucosal specimens removed from the inferior promontory area of 19 patients undergoing middle ear surgery for COM were studied.. Methods Sections were stained with H&E, Alcian blue-periodic acid Schiff (AB-PAS), polyclonal MUC5B antibody, and specific MUC5B riboprobe for histological, histochemical, immunohistochemical, and mucin mRNA analyses.. Results H&E staining revealed pseudostratified epithelia in 18 of the middle ear specimens with COM and cuboidal secretory epithelia in one. AB-PAS staining of epithelia revealed abundant secretory cells and their products (glycoconjugates). In situ hybridization and immunohistochemistry studies demonstrated that the secretory cells of the middle ear mucosa ...
0005] The glycocalyx of the corneal epithelium contains the transmembrane mucin (glycosylated glycoproteins present in the glycocalyx), MUC1, MUC4, and MUC16. Mucins are high molecular weight Glycoproteins that provide a protective layer on corneal epithelial surfaces and are involved in cell-cell interactions, signaling, and metastasis. MUCIN 1, a cell surface associated (MUC 1) or polymorphic epithelial mucin (PEM) is a mucin, the protein part of which is encoded by the MUC1 gene in humans. Mucin proteins penetrate the membranes of epithelial cells, on the inner surface of the intestine and other organs. Mucin protects the body from infection by binding to pathogens. These membrane mucins interact with soluble, secreted, gel-forming mucin produced by the goblet cells (MUC5AC) and also with others like MUC2. The lacrimal gland also secretes MUC7 into the tear film. These soluble mucin in the tear film moves freely, (made possible by blinking and electrostatic repulsion from the negatively ...
Human tracheobronchial epithelial cells have been serially passaged in serum-free medium. This serum-free model was employed to investigate the effects of different concentrations of Ca2+ (0.1, 1.0 and 2.0 mM) on multiplication and morphology of the cells. The responses were analysed in terms of growth kinetics, histochemical and ultrastructural alterations. Culturing of the cells in high Ca2+ (1.0-2.0 mM) medium stimulated cell multiplication characterized by increased colony forming efficiency, greater number of cells per colony and cell population doublings per day. Additionally, the high Ca2+ concentrations induced proliferation in cultures grown to confluency in low Ca2+ (0.1 mM) medium. Cells propagated in low Ca2+ medium consisted of relatively heterogeneous cell populations, with most cells staining positive with periodic acid-Schiff (PAS) reagent. Ultrastructurally the cells exhibited secretory vesicles and microvilli on their surfaces, small desmosomes and intercellular interdigitation ...
Mucins are high-molecular weight glycoproteins (0.25-20 MDa) containing one or more domains that are heavily O-glycosylated. Their implications as targets for cancer treatment have increased the interest in these glycoproteins, mainly in the fields of vaccines and antibodies. However, mucins present high heterogeneity, posing challenges that affect purification processes and quality control analysis. In that sense, it is necessary to develop and improve downstream processes and analytical methods to characterize these products. Here a tool based on biolayer interferometry analysis to improve mucins detection and quantification in a fast, simple and label free-way is presented. Taking advantage of lectin recognition of mucins carbohydrate structures, several lectins were evaluated and immobilized on streptavidin biosensors. Different assay conditions were optimized and the most suitable lectin, Aleuria aurantia lectin (AAL), was selected. Bovine Submaxillary Gland and human MUC5B mucins were ...
Changes in the expression of mucin genes in gastrointestinal cancers is thought to contribute to the development of the disease. In our laboratory we have shown previously that MUC5AC is aberrantly expressed in rectosigmoid villous adenomas. However, the regulatory mechanisms underlying that altered profile of expression is unknown. In order to study its regulation at the transcriptional level, we have isolated and characterized 5.5 kb of the 5´-flanking region of the mouse Muc5ac mucin gene. The promoter is flanked by a TATA box and a transcriptional start site is located 22 bp downstream of the TATA box. Analysis of the sequence showed a high density of binding sites for Smad4, an essential factor in the signalling cascade activated by TGF-β (transforming growth factor-β), and Sp1, an important factor in the regulation of MUC5AC. This led us to study Muc5ac regulation by TGF-β. We show that exogenous addition of TGF-β to the cells induces Muc5ac endogenous expression, promoter activity ...
To investigate the genetic identities of the mucins secreted in cystic fibrosis (CF) airways, sputum was collected from five individuals. Samples were separated into gel and sol phases by high-speed centrifugation and the gel phase was extracted in 6 M guanidinium chloride. The insoluble residue remaining after extraction of the gel phase was brought into solution by reduction/alkylation. Density-gradient centrifugation in CsCl revealed polydisperse distributions of sialic acid-containing mucins in the gel phase, insoluble residue and sol phase fractions and the degree of variation between the different individuals was low. Antibodies recognizing MUC5AC and MUC5B identified these mucins in each of the fractions. MUC2, however, was present only as a component of the insoluble residue from the gel which accounted for less than 4% by mass of the total mucins. MUC5B and MUC5AC from the gel phase were large oligomeric species composed of disulphide-bond linked subunits and MUC5B was present as two ...
The sustained delivery of both hydrophobic and hydrophilic drugs from hydrogels remains a challenge that requires the design of complex multifunctional synthetic polymers. In this work we exploit mucin glycoproteins, the gel-forming building blocks of natural mucus to deliver both hydrophobic and hydrophilic drugs. Mucins are a family of high molecular weight proteins which are densely glycosylated. Their central protein backbone contains hydrophobic and charged domains, while the mucin-associated glycans provide hydrogen bonding capabilities, high hydration, and negative charges. The biochemical versatility of mucins represents potential binding sites for certain drugs. If assembled into hydrogels, mucins could prevent drugs from freely diffusing out, leading to their sustained delivery. We generated methacrylated mucins which assembled into a covalently crosslinked mucin hydrogel when exposed to UV light. The rheological properties of the mucin gels were dominated by an elastic component, and ...
This gene encodes a small salivary mucin, which is thought to play a role in facilitating the clearance of bacteria in the oral cavity and to aid in mastication, speech, and swallowing. The central domain of this glycoprotein contains tandem repeats, each composed of 23 amino acids. This antimicrobial protein has antibacterial and antifungal activity. The most common allele contains 6 repeats, and some alleles may be associated with susceptibility to asthma. Alternatively spliced transcript variants with different 5 UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Oct 2014 ...
The MACS GMP PepTivator Mucin-1 is a peptide pool that consists mainly of 15-mer peptides. It has been developed for efficient in vitro stimulation and subsequent isolation of Mucin-1-specific CD4+ and CD8+ T cells. - USA
Cystic fibrosis (CF) lung disease is characterized by early and persistent mucus accumulation and neutrophilic inflammation in the distal airways. Identification of the factors in CF mucopurulent secretions that perpetuate CF mucoinflammation may provide strategies for novel CF pharmacotherapies. We show that IL-1β, with IL-1α, dominated the mucin prosecretory activities of supernatants of airway mucopurulent secretions (SAMS). Like SAMS, IL-1β alone induced MUC5B and MUC5AC protein secretion and mucus hyperconcentration in CF human bronchial epithelial (HBE) cells. Mechanistically, IL-1β induced the sterile α motif-pointed domain containing ETS transcription factor (SPDEF) and downstream endoplasmic reticulum to nucleus signaling 2 (ERN2) to upregulate mucin gene expression. Increased mRNA levels of IL1B, SPDEF, and ERN2 were associated with increased MUC5B and MUC5AC expression in the distal airways of excised CF lungs. Administration of an IL-1 receptor antagonist (IL-1Ra) blocked ...
Cystic fibrosis (CF) lung disease is characterized by early and persistent mucus accumulation and neutrophilic inflammation in the distal airways. Identification of the factors in CF mucopurulent secretions that perpetuate CF mucoinflammation may provide strategies for novel CF pharmacotherapies. We show that IL-1β, with IL-1α, dominated the mucin prosecretory activities of supernatants of airway mucopurulent secretions (SAMS). Like SAMS, IL-1β alone induced MUC5B and MUC5AC protein secretion and mucus hyperconcentration in CF human bronchial epithelial (HBE) cells. Mechanistically, IL-1β induced the sterile α motif-pointed domain containing ETS transcription factor (SPDEF) and downstream endoplasmic reticulum to nucleus signaling 2 (ERN2) to upregulate mucin gene expression. Increased mRNA levels of IL1B, SPDEF, and ERN2 were associated with increased MUC5B and MUC5AC expression in the distal airways of excised CF lungs. Administration of an IL-1 receptor antagonist (IL-1Ra) blocked ...
Cystic fibrosis (CF) lung disease is characterized by early and persistent mucus accumulation and neutrophilic inflammation in the distal airways. Identification of the factors in CF mucopurulent secretions that perpetuate CF mucoinflammation may provide strategies for novel CF pharmacotherapies. We show that IL-1β, with IL-1α, dominated the mucin prosecretory activities of supernatants of airway mucopurulent secretions (SAMS). Like SAMS, IL-1β alone induced MUC5B and MUC5AC protein secretion and mucus hyperconcentration in CF human bronchial epithelial (HBE) cells. Mechanistically, IL-1β induced the sterile α motif-pointed domain containing ETS transcription factor (SPDEF) and downstream endoplasmic reticulum to nucleus signaling 2 (ERN2) to upregulate mucin gene expression. Increased mRNA levels of IL1B, SPDEF, and ERN2 were associated with increased MUC5B and MUC5AC expression in the distal airways of excised CF lungs. Administration of an IL-1 receptor antagonist (IL-1Ra) blocked ...
Beatson, Richard, Tajadura-Ortega, Virginia, Achkova, Daniela, Picco, Gianfranco, Tsourouktsoglou, Theodora-Dorita, Klausing, Sandra, Hillier, Matthew, Maher, John, Noll, Thomas, Crocker, Paul R, Taylor-Papadimitriou, Joyce, and Burchell, Joy M. "The mucin MUC1 modulates the tumor immunological microenvironment through engagement of the lectin Siglec-9". Nature Immunology 17.11 (2016): 1273-1281 ...
This graph shows the total number of publications written about "Mucin-2" by people in this website by year, and whether "Mucin-2" was a major or minor topic of these publications ...
Rarely do animals expectorate material, as most material is swallowed, and so recognising a productive cough (excess airway mucus) can be difficult. During paroxysms of coughing, saliva can accumulate in the pharynx and may be expectorated with a retching action. This material should not be confused with airway mucus. ...
A critical function of mucin in saliva is to protect the mouth from drying out. Mucin also lubricates the tissues and teeth in the mouth and protects them against pathogens and...
Kesimer M., Scull M., Brighton B., DeMaria G., Burns K., ONeal W., Pickles R.J., Sheehan J.K.. Airway mucus forms the structural basis of the local innate immune defense mechanism. It is an integrated, active, viscoelastic gel matrix evolved to protect the exposed lung from physical, chemical, and pathological erosion. Exosomes are biologically active vesicles secreted by different cell types including epithelial, hematopoietic, and some tumor cells. They are also present in some biological fluids such as serum, urine, breast milk, and bronchoalveolar lavage fluid. In this study, we demonstrate for the first time that exosome-like vesicles with antiviral properties are present in human tracheobronchial epithelial (HTBE) cell culture secretions. These vesicles have been isolated by differential centrifugation and are characterized further by mass spectrometry, flow cytometry, immunoblotting, electron microscopy, and light-scattering methods. HTBE vesicles exhibited characteristic exosomal size ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. [provided by RefSeq, Jul 2010 ...