Background The National Comprehensive Cancer Network guidelines recommend that patients with oral cavity squamous cell carcinoma (OSCC) and cT4b disease should be either included in clinical trials or...
TY - JOUR. T1 - Metabolomic profiling of sodium fluoride-induced cytotoxicity in an oral squamous cell carcinoma cell line. AU - Sakagami, Hiroshi. AU - Sugimoto, Masahiro. AU - Tanaka, Shoji. AU - Onuma, Hiromi. AU - Ota, Sana. AU - Kaneko, Miku. AU - Soga, Tomoyoshi. AU - Tomita, Masaru. PY - 2014/4/1. Y1 - 2014/4/1. N2 - Sodium fluoride (NaF) is used in dentistry as a preventive agent for dental caries because of its ability to remineralize the tooth surface and its antibacterial effect. Although one of its target molecules in bacteria is enolase, its site of action in human cells has not been identified. The aim of this study was to identify target metabolites that are coupled to NaF-induced cytotoxicity in the HSC-2 human oral squamous cell carcinoma cell line. Cell viability, membrane integrity and apoptosis induction were analyzed by MTT assay, trypan blue exclusion and caspase-3 activation, respectively. Cells were treated with a minimal cytotoxic concentration of NaF for various times ...
Cisplatin is widely used for chemotherapy of head and neck squamous cell carcinoma. However, details of the molecular mechanism responsible for cisplatin resistance are still unclear. The aim of this study was to identify the expression of genes related to cisplatin resistance in oral squamous cell carcinoma cells. A cisplatin-resistant cell line, Tca/cisplatin, was established from a cisplatin-sensitive cell line, Tca8113, which was derived from moderately-differentiated tongue squamous cell carcinoma. Global gene expression in this resistant cell line and its sensitive parent cell line was analyzed using Affymetrix HG-U95Av2 microarrays. Candidate genes involved in DNA repair, the MAP pathway and cell cycle regulation were chosen to validate the microarray analysis results. Cell cycle distribution and apoptosis following cisplatin exposure were also investigated. Cisplatin resistance in Tca/cisplatin cells was stable for two years in cisplatin-free culture medium. The IC50 for cisplatin in Tca
Cisplatin is widely used for chemotherapy of head and neck squamous cell carcinoma. However, details of the molecular mechanism responsible for cisplatin resistance are still unclear. The aim of this study was to identify the expression of genes related to cisplatin resistance in oral squamous cell carcinoma cells. A cisplatin-resistant cell line, Tca/cisplatin, was established from a cisplatin-sensitive cell line, Tca8113, which was derived from moderately-differentiated tongue squamous cell carcinoma. Global gene expression in this resistant cell line and its sensitive parent cell line was analyzed using Affymetrix HG-U95Av2 microarrays. Candidate genes involved in DNA repair, the MAP pathway and cell cycle regulation were chosen to validate the microarray analysis results. Cell cycle distribution and apoptosis following cisplatin exposure were also investigated. Cisplatin resistance in Tca/cisplatin cells was stable for two years in cisplatin-free culture medium. The IC50 for cisplatin in Tca
The purpose of the present study was to describe the survival of patients diagnosed with oral cavity cancer in Germany. The analyses relied on data from eleven population-based cancer registries in Germany covering a population of 33 million inhabitants. Patients with a diagnosis of oral cavity cancer (ICD-10: C00-06) between 1997 and 2006 are included. Period analysis for 2002-2006 was applied to estimate five-year age-standardized relative survival, taking into account patients sex as well as grade and tumor stage. Overall five-year relative survival for oral cavity cancer patients was 54.6%. According to tumor localization, five-year survival was 86.5% for lip cancer, 48.1% for tongue cancer and 51.7% for other regions of the oral cavity. Differences in survival were identified with respect to age, sex, tumor grade and stage. The present study is the first to provide a comprehensive overview on survival of oral cavity cancer patients in Germany.
Our aim was to document the health-related quality of life (QoL) of patients with squamous cell carcinoma (SCC) of the oral cavity who were treated with chemoradiotherapy, and to compare it with that of patients treated with conventional surgery with or without adjuvant treatment. All patients who presented with SCC of the oral cavity treated with chemoradiotherapy alone at the Royal Brisbane & Womens Hospital between 2000 and 2011 and who were alive without disease were included. Health-related QoL was assessed by the University of Washington QoL questionnaire version 4, and the European Organisation for the Research and Treatment of Cancer (EORTC) QoL questionnaires C30 and HN35. The questionnaires were sent to all survivors. Those who responded to chemoradiotherapy were matched with patients who were treated by conventional surgery with or without adjuvant treatment by age, sex, subsite of tumour, and TNM stage. Sixteen patients completed the questionnaires (8 in each group). There was no ...
Telomerase is a ribonucleoprotein enzyme that maintains telomere length. Telomerase activity is primarily attributed to the expression of telomerase reverse transcriptase (TERT). It has been reported that introduction of an intact human chromosome 3 into the human oral squamous cell carcinoma cell line HSC3 suppresses the tumorigenicity of these cells. However, the mechanisms that regulate tumorigenicity have not been elucidated. To determine whether this reduction in tumorigenicity was...
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TGF-β1rs1982073 polymorphism at the miRNA-187 binding site may alter TGF-β1 expression and function, and thereby this polymorphism (genotype CT/CC) increases cancer susceptibility. HPV16 L1 seropositivity is associated with the risk of oral squamous cell carcinoma (OSCC), including oropharyngeal squamous cell carcinoma (OPSCC) and oral cavity squamous cell carcinoma (OCSCC). Thus, we hypothesized that TGF-β1rs1982073 polymorphism at the miRNA-187 binding site combined with HPV16 L1 seropositivity may have a joint effect on OSCC susceptibility. We determined the genotypes of TGF-β1rs1982073 and HPV16 status in 325 OSCC subjects and 335 cancer-free controls in the non-Hispanic white population, and used logistic regression models to evaluate the joint effects on OSCC susceptibility. TGF-β1rs1982073 polymorphism (CT/CC genotype) combined with HPV16 L1 seropositivity increased the risk of OSCC via joint effects, particularly in OPSCC subjects who were never-smokers (OR, 165.9; 95% CI, ...
Induction chemotherapy is regarded as an effective way to reduce or downgrade the locally advanced or aggressive cancers, and to improve the chance of eradication of the locoregional lesions by radical surgery and/or radiotherapy. However, there are still debates on the clinical value of induction chemotherapy for patients with advanced and resectable oral squamous cell carcinoma. The hypothesis of this study is that the induction chemotherapy of TPF (docetaxel, cisplatin, and 5-fluorouracil) protocol could benefit the patients with locally advanced oral squamous cell carcinoma. The endpoints of this study are the survival rate, local control, and safety ...
Induction chemotherapy is regarded as an effective way to reduce or downgrade the locally advanced or aggressive cancers, and to improve the chance of eradication of the locoregional lesions by radical surgery and/or radiotherapy. However, there are still debates on the clinical value of induction chemotherapy for patients with advanced and resectable oral squamous cell carcinoma. The hypothesis of this study is that the induction chemotherapy of TPF (docetaxel, cisplatin, and 5-fluorouracil) protocol could benefit the patients with locally advanced oral squamous cell carcinoma. The endpoints of this study are the survival rate, local control, and safety ...
This study was performed to evaluate their 5-year survival rates and identify the factors affecting the prognosis of oral cancer patients who had undergone surgical treatment only. Among 130 patients who were diagnosed with malignant tumor of oral, maxillofacial, and surgical treated in the Department of Oral and Maxillofacial Surgery at Chonnam National University Hospital within a period from January 2000 to December 2010, for 11 years, 84 patients were investigated who were followed up for more than 5 years after radical surgery; oral cancer is primary and received only surgical treatment. The survival rate according to gender, age, type and site of cancer, TNM stage, cervical lymph node metastasis and its stage, recurrence or metastasis, time of recurrence and metastasis, and differentiation were investigated and analyzed. Overall, 5-year survival rate in patients who received only surgical treatment was 81.2 %, and disease-specific 5-year survival rate was 83.1 %. The disease-specific 5-year
We have identified 131 probe sets, corresponding to 108 known genes, which are highly effective in distinguishing invasive OSCC and normal oral tissue, as well as a list of genes that might be involved in the transformation of normal oral tissue to dysplasia, and of oral dysplasia to invasive OSCC. Although prior studies, including our own, have described global changes in gene transcription that distinguish normal oral epithelium from carcinoma, there is considerable heterogeneity among the lists of genes that have been reported and, to our knowledge, few studies have produced a limited combinations of genes as in the current study with high sensitivity and specificity in distinguishing OSCC from normal oral tissue through rigorous statistical testing and validation with independent data sets, and none had provided prediction models (14). The current study provides prediction models that were generated using rigorous statistical analyses, and the differences in gene expression detected using ...
OBJECTIVE: Oral squamous cell carcinoma (OSCC) is a common tumor of head and neck cancer. MiR-103 is involved in several tumors. However, the role and
In the present study, we evaluated the proliferative behavior of oral SCC cell lines including cetuximab-sensitive HSC3 and HSC4 and cetuximab-resistant SAS. Notably, all cell lines expressed EGFR in the cell membrane and phosphorylated, regardless of cetuximab sensitivity status.. The monoclonal antibody cetuximab targets the extracellular domain of EGFR with high specificity and affinity (37). Cetuximab blocks ligand binding and thereby inhibits EGFR phosphorylation (9). Thus, cetuximab should inhibit EGFR-dependent cell proliferation. In the present study, proliferation of HSC3 and HSC4 cells was strongly associated with EGFR ligand-EGFR signaling, since proliferation was markedly reduced by the EGFR kinase inhibitor AG1478. Thus, cetuximab prevented EGFR phosphorylation, reducing proliferation of HSC3 and HSC4. These data are consistent with those of a previous report that EGFR biomarker analysis in non-small cell lung carcinoma patients showed that those with higher EGFR expression levels ...
BOSTON-Three species of bacteria were found at significantly high levels in the saliva of oral cancer patients, suggesting that the organisms might serve as markers of the disease.
A primary or metastatic malignant neoplasm affecting the head and neck. Representative examples include oral cavity squamous cell carcinoma, laryngeal squamous cell carcinoma, and salivary gland carcinoma.
Higher nodal yield during definitive surgery for clinically node-negative oral cavity squamous cell carcinoma was associated with improved mortality. 1
Despite recent advances in oral squamous cell carcinoma (OSCC) diagnosis and therapy, disease recurrence remains common and is strongly associated with mortality. It is therefore critical to identify new targets for both treatment and diagnostic purposes. We aimed at investigating the role of PA28α, a proteasomal activator in OSCC. The expression of PA28α was examined in a panel of OSCC cell lines and tissues, associated with oncomine analysis. In a large OSCC patient cohort, the prognostic value of PA28α expression was evaluated. Primary clinical end points were recurrence-free and overall survival rate. Functional involvement of PA28α in OSCC was examined in both in vitro and in vivo models upon specific siRNA knockdown. PA28α was found to be overexpressed in OSCC cell lines and tumor tissues. High expression of PA28α was significantly associated with recurrence and poorer overall survival. Specific knockdown of PA28α inhibited OSCC cell proliferation, migration, invasion in vitro and reduced
This is a 60-year-old male with a history of stage 4B oral cavity squamous cell carcinoma. The patient underwent induction chemo with only partial response; however, following induction chemotherapy, he was noted to have resectable disease. The decision was made to take the patient to the operating room for composite resection of his left buccal lesion including left hemimandibulectomy, left neck dissection, tracheostomy, left fibular free flap, left split-thickness skin graft. Following the surgery, the patient was noted to have good Doppler signal and had good response to the pinprick test; however, one to two hours following transfer to the recovery room, the patient was noted to have lost his Doppler signal. At this point, _____ and there was noted to be decreased blood. Given the concern for possible flap compromise, a ...
Here are recent publications with contributions from K2 based on last weeks search on PubMed (and optionally articles that have not been included in previous lists). This time the list includes in total 20 recent publications. The entries appear in the order they were received from NCBI. If you have publications that are not included in this or previous lists, please send the references to Hege F. Berg.. 1. Tyrosine kinase inhibitors and interferon-α increase tunneling nanotube (TNT) formation and cell adhesion in chronic myeloid leukemia (CML) cell lines.. Omsland M, Andresen V, Gullaksen SE, Ayuda-Durán P, Popa M, Hovland R, Brendehaug A, Enserink J, McCormack E, Gjertsen BT.. FASEB J. 2020 Jan 16. doi: 10.1096/fj.201802061RR. [Epub ahead of print]. PMID: 31945226. 2. Characteristics and prognosis of primary treatment-naïve oral cavity squamous cell carcinoma in Norway, a descriptive retrospective study.. Bjerkli IH, Jetlund O, Karevold G, Karlsdóttir Á, Jaatun E, Uhlin-Hansen L, ...
Oral Cancer Screening Downtown Toronto, Possible Signs of Oral Cancer, Oral Cancer Check-up, Diagnosis and Treatment, Oral Cancer Prevention, Norton Dental Downtown
FOXM1 has vital roles in adult tissue homeostasis, cell proliferation, cell differentiation, apoptosis and aging as well as in the pathogenesis of human cancers (16,17,26). Normal cells show a lower expression of FOXM1 than cancer cells. Dysfunction of FOXM1 inhibits cell differentiation, which may finally lead to the malignant transformation of undifferentiated cells (27). Moreover, upregulated expression of FOXM1 has been observed in a number of cancers including hepatocellular carcinoma, breast cancer, non-small cell lung carcinomas and glioblastomas as well as prostate, cervical and gastric cancer (21,28-33). Recent studies have strongly suggested that overexpression of FOXM1 could be correlated with cancer progression and might be a potential prognostic biomarker for cancer patients (28-33). In addition, the prognostic significance of FOXM1 expression is clearly seen in various cancers including renal, liver, pancreatic and lung cancer, by using The Cancer Genome Atlas or The Human Protein ...
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Oral squamous cell carcinoma (OSCC) is a major health problem in many developing countries, representing more than 25% of all new cancer cases in some countries such as India and Sri Lanka. Cigarette smoking, smokeless tobacco (ST), alcohol use and chewing of betel quid (BQ) are the main risk factors associated with OSCC development. Infection with highrisk Human papillomavirus (HPV) is an emerging risk factor, particularly for oropharyngeal cancers. Most of the OSCC cases are diagnosed at advanced stages, being one of the factors related to the high mortality rate of this cancer. A better understanding of the molecular biology of OSCC development might lead to improved methods related to detection, assessing prognosis and novel treatments of this malignancy. Over the recent years, microarray-based technologies have become commonly used techniques for analyzing gene expression and chromosomal alterations in human cancers and other disease conditions. These high-throughput technologies enable ...
The tumor suppressor Programmed Cell Death 4 (PDCD4) has been found to be under-expressed in several cancers and associated with disease progression and metastasis. There are no current studies characterizing PDCD4 expression and its clinical relevance in Oral Squamous Cell Carcinoma (OSCC). Since nodal metastasis is a major prognostic factor in OSCC, we focused on determining whether PDCD4 under-expression was associated with patient nodal status and had functional relevance in OSCC invasion. We also examined PDCD4 regulation by microRNA 21 (miR-21) in OSCC. PDCD4 mRNA expression levels were assessed in 50 OSCCs and 25 normal oral tissues. PDCD4 was under-expressed in 43/50 (86%) OSCCs, with significantly reduced mRNA levels in patients with nodal metastasis (p = 0.0027), and marginally associated with T3-T4 tumor stage (p = 0.054). PDCD4 protein expression was assessed, by immunohistochemistry (IHC), in 28/50 OSCCs and adjacent normal tissues; PDCD4 protein was absent/under-expressed in 25/28 (89%)
Recent studies have shown an increase in the incidence of oral squamous cell carcinoma (OSCC) in younger patients. The hypothesis that tumors could be hormonally induced during pregnancy or in young female patients without the well-known risk factors alcohol or tobacco abuse seems to be plausible. Estrogen Receptor alpha (ER?) and Progesterone Receptor (PR) expression were analyzed in normal oral mucosa (n=5), oral precursor lesions (simple hyperplasia, n=11; squamous intraepithelial neoplasia, SIN I-III, n=35), and OSCC specimen. OSCCs were stratified in a young female (n=7) study cohort and older patients (n=46). In the young female study cohort three patients (n=3/7) developed OSCC during or shortly after pregnancy. Breast cancer tissues were used as positive control for ER? and PR expression. ER? expression was found in four oral precursor lesions (squamous intraepithelial neoplasia, SIN I-III, n=4/35, 11%) and in five OSCC specimen (n=5/46, 11%). The five ER? positive OSCC samples were ...
Overexpression of EGFR has been documented in OSCC (28,29). EGFR signaling is associated with a loss of cell adhesion mediated by E-cadherin and acquisition of a fibroblast-like cellular morphology, which subsequently increases cell motility and potentially serves a role in tumor invasion and metastasis (14,30,31). In the present study, morphological changes in HSC-3 cells and reductions in cell motility were observed following EGFR inhibition. Treatment of cells with a low concentration of EGFR inhibitor induced the formation of cell-cell junctions, indicated by an accumulation of E-cadherin and ZO-1 at cell-cell contacts and strengthening of barrier function. Suppression of EGFR expression by siRNA also induced cellular morphological changes and accumulation of E-cadherin at cell-cell contacts. The HSC-3 cells used in the present study are generally poorly differentiated cells with a random morphology, have exhibit a limited ability to form cell junctions within monolayer cultures (32). ...
Purpose: The aim of this study was to investigate the diagnostic delay and its determinants among oral cancer patients in Tehran, Iran. Materials and Methods: This study was conducted between September 2004 and September 2006 in three university hospitals, and included 100 consecutive patients with primary oral squamous cell carcinoma (international classification of disease, ICD-10 sites C01 to C06). Data were obtained through questionnaire interviews and medical records of the patients were reviewed to obtain information on the date of diagnosis, primary tumour site and the stage of the tumour at the time of diagnosis. Statistical analysis was performed by t test, ANOVA and logistic regression. Results: The mean diagnostic delay was 7.2 months (SD 7.5, range 1 to 36 and median 4). The most important determinants of longer diagnostic delay were being single (OR = 4.8; 95% CI = 1.5 to 14.8; P < 0.05) and being at advanced tumour stages (OR = 5.3; 95% CI = 1.8 to 15.6; P < 0.01). The mean patient ...
Sigma-Aldrich offers abstracts and full-text articles by [Karin Bauer, Martin Gosau, Jörg Reinders, Peter Oefner, Torsten E Reichert, Richard Bauer].
Current organotypic models of dysplasia and oral squamous cell carcinoma (OSCC) lack the complexity that mimics in vivo tissue. Here we describe a three-dimensional in vitro model of the oral epithelium that replicates tumour progression from dysplas
Oral cancers account for approximately 3 percent of all cases of cancer in the United States. An estimated 30,000 people will be diagnosed with oral cancer this year, and about one half of them will eventually die of the disease. The most common type of oral cancer is squamous cell carcinoma. Sixty percent of oral cancers are well advanced by the time they are detected, even though physicians and dentists frequently examine the oral cavity. The two most important risk factors for oral cancer are tobacco use and heavy alcohol consumption. The keys to reducing mortality are prevention and control. The earlier any intraoral or extraoral abnormalities or lesions are detected and biopsied, the more lives can be saved. Controversy exists whether screening programs effectively reduce the mortality rate. Specific step-by-step guidelines should be followed to perform an adequate examination of the head and neck.
Tumor-associated macrophages (TAMs) are a major cellular component in the tumor microenvironment of many solid tumors. The functional competence of TAMs varies depending on the type of tumors and their respective microenvironments. The classically activated M1 macrophages exhibit antitumor functions, whereas the alternatively activated M2 macrophages exhibit protumor functions that contribute to tumor development and progression. Although TAMs have been detected in oral squamous cell carcinoma (OSCC), little is known about their phenotype. In the present study, we performed an immunohistochemical analysis to identify TAMs in surgically resected specimens from 50 patients with OSCC and evaluated the relationship between infiltrated TAMs and the pathological grade of OSCC. Positive staining for CD163, which has been used as a marker for M2 macrophages, was observed in OSCC specimens, and the percentages of CD163+ cells were significantly increased based on the pathological grade. CD163+ cells were
The present study shows significant increase in lipid peroxides in OSCC patients and in tobacco chewers/smokers group. The increase in MDA is very highly significant in OSCC patients compared to normal healthy control subjects. Oxygen derived free radicals known as reactive oxygen species (ROS) are involved in neoplastic transformation [9]. The role of ROS in the initiation, promo-tion and progression of carcinogenesis and the protective role of antioxidant enzymes has been the subject of much speculation with conflicting reports in literature. Nagini, Manoharan, Ramchandran [10] have reported significantly decreased lipid peroxidation levels in oral cancer patients. Their findings suggest a decreased susceptibility of oral tumour tissue to lipid peroxidation. Tumour progression is associated with low levels of malondialdehyde [11,12]. An inverse relationship has been observed between the levels of lipid peroxidation and the rate of cell proliferation [13].. In contrast, enhanced lipid ...
Introduction: As one of the most common cancers among head and neck malignancies, cancer of the oral cavity probably has some variations in countries with a high prevalence of esophageal cancer. Materials and Methods: Patients with oral cavity cancer who were treated at two tertiary referral centers from January 1999 to January 2009 were included in this study. In addition to demographic data, information regarding personal and family history of head and neck cancer, use of dentures, presence of immune deficiency, consumption of alcohol, and incidence of cigarette smoking was collected. Additionally, a history of opium usage was obtained from the participants in this study. Moreover, an appropriately matched control group was selected for comparisons between the risk factors. Results: A total of 557 patients were entered into this study over a 10-year period, of whom 219 (39.3%) were female and the remaining 338 (60.7%) were male. The tongue was the most common site of cancer and 9% of the patients
Oral Squamous Cell Carcinoma (OSCC) is a cancer of the mouth epithelium with an annual incidence rate in the US of nearly 48,000 cases annually and a 5-year survival rate of only 65%. Cancer stem cells (CSCs) are believed to be responsible for progression of OSCC. The projects main goal is to observe the effect of Wnt/β-catenin signaling inhibition on oral squamous cell carcinoma.. ...
Treatment protocols for oral cavity cancers are provided below, including generalized first-line therapy based on stage; chemoradiation therapy and induction chemotherapy for locally advanced disease; and first-, second-, and third-line chemotherapy for metastatic or recurrent disease. Generalized treatment recommendations for oral cavity ca...
Oral cavity cancers refer to malignant tumors of the oral mucosa, tonsils, and salivary glands. Predisposing factors include smoking, oral tobacco consumption,…
The way the radiation therapy is given depends on the type and stage of the cancer being treated. External and internal radiation therapy are used to treat lip and oral cavity cancer.. Radiation therapy may work better in patients who have stopped smoking before beginning treatment. It is also important for patients to have a dental exam before radiation therapy begins, so that existing problems can be treated.. New types of treatment are being tested in clinical trials. This summary section describes treatments that are being studied in clinical trials. It may not mention every new treatment being studied. Information about clinical trials is available from the NCI website.. Chemotherapy Chemotherapy is a cancer treatment that uses drugs to stop the growth of cancer cells, either by killing the cells or by stopping the cells from dividing. When chemotherapy is taken by mouth or injected into a vein or muscle, the drugs enter the bloodstream and can reach cancer cells throughout the body ...
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Purpose: The hypermetabolic nature of cancer cells, especially their increased reliance on aerobic glycolysis which has been associated with more aggressive phenotype, is considered metabolic hallmarks of cancer cells including oral squamous cell carcinoma (OSCC) cells. However, its precise mechanisms remain unknown. Cylindromatosis (CYLD) is recognized as a tumor suppressor gene whereas little is available about its impact on cancer progression. Our unpublished data showed that lower CYLD expression was associated with poor prognosis in OSCC patients. The objective of our study was to address specific contributions of CYLD to the signature metabolic features of OSCC cells.. Materials and methods: We determined the level of glucose consumption as well as lactate production to evaluate the effects of CYLD knockdown by siRNA on aerobic glycolysis in human OSCC cell lines. In addition, we measured extracellular acidification and oxygen consumption rates in OSCC cells by using XF Extracellular Flux ...
Understanding how optical properties are altered during oral carcinogenesis is critical for optimizing diagnostic technologies for oral cancer detection based on autofluorescence imaging and spectroscopy. In this study, we used high-resolution microscopy to investigate patterns of autofluorescence in normal oral mucosa and in benign and neoplastic oral lesions. Our results show that the autofluorescence properties of oral tissue vary based on the anatomic site within the oral cavity and the pathologic diagnosis. The fluorescence signals from epithelial and stromal layers can change independently of other tissue layers. This has important implications for the clinical diagnosis of oral lesions using fluorescence imaging and spectroscopy.. When normal oral tissue is illuminated by UV light, most of the epithelial autofluorescence that is generated originates from the cytoplasm of cells occupying the basal and intermediate layers. Similar findings were found using confocal images of cervical ...
MicroRNAs (miRNAs) participate in many biological processes and have emerged as key players in carcinogenesis. In order to identify changes in miRNAs specific for betelquid-associated oral squamous cell carcinoma (OSCC), we investigated the expression profiles of 858 miRNAs in a panel of 40 pairs of OSCC specimens and their matched non-tumorous epithelial counterparts. Eighty-four miRNAs were differentially expressed in the OSCC specimens compared to the matched tissue. Among these miRNAs, 19 downregulated miRNAs located in the chromosome 14q32.2 miRNA cluster region were analyzed. This miRNA cluster resides within a parentally imprinted region known to be important in development and growth. The re-expression of miR-329 and miR-410from this cluster significantly reduced OSCC cell proliferation and invasion and downregulated Wnt-7b, an activator of the Wnt/b-catenin pathway. Furthermore, the expression of those 2 miRNAs was restored by 5-aza-2′-deoxycytidine treatment and the expression levels of the
885 Management of head and neck cancer is complicated by local recurrence and the development of second primaries. Following diagnosis of one primary oral squamous cell carcinoma (SCC) the annual rate for development of a second primary SCC is 4-7% and the 5-year survival rate is reduced to 25%. Second primary oral SCCs are hypothesized to be; (a) independent tumors that developed from unrelated cells, possibly within a field of cells predisposed due to environmental agents such as tobacco smoke (field cancerization), (b) "second field tumors" or "clonal cancers" that developed from a field or patch of aberrant cells that had spread to encompass large areas of the oral cavity, or (c) tumors established by cells sloughed or migrating from a previous tumor. Since tumor genomes typically acquire multiple alterations, the relatedness of multiple tumors can be evaluated based on whether or not they share particular alterations. Thus, tumors arising by the first mechanism would be likely to share only ...
Cancer is a scary word, but the more you know about it, the better able you will be to protect yourself and the ones you love. This is particularly true of oral cancer, which is very treatable if caught early. Unfortunately, about two-thirds of oral cancers are not caught until the late stages. You may think that if you are a non-smoker, particularly a young one, this topic is not of concern to you. If so, please think again.. While most oral cancer patients are smokers, the fastest-growing segment of newly diagnosed cases is young, non-smoking adults. The culprit is a particular strain of the Human Papilloma Virus (HPV). HPV is the most common sexually transmitted disease in the U.S., with about 20 million Americans infected. In fact, it is estimated that at least half of all sexually active people will contract it during their lives. Most strains (and there are over 100) lead to symptoms no more serious than warts, and in many cases a persons own immune system can rid the body of the disease ...
Cancer is a scary word, but the more you know about it, the better able you will be to protect yourself and the ones you love. This is particularly true of oral cancer, which is very treatable if caught early. Unfortunately, about two-thirds of oral cancers are not caught until the late stages. You may think that if you are a non-smoker, particularly a young one, this topic is not of concern to you. If so, please think again.. While most oral cancer patients are smokers, the fastest-growing segment of newly diagnosed cases is young, non-smoking adults. The culprit is a particular strain of the Human Papilloma Virus (HPV). HPV is the most common sexually transmitted disease in the U.S., with about 20 million Americans infected. In fact, it is estimated that at least half of all sexually active people will contract it during their lives. Most strains (and there are over 100) lead to symptoms no more serious than warts, and in many cases a persons own immune system can rid the body of the disease ...
UCLA School of Dentistry researchers have substantiated the effectiveness of measuring the microRNAs in saliva to detect oral squamous cell carcinoma. Two particular microRNAs, miR-125a and miR-200a, are present at significantly different levels in the saliva of individuals suffering from OSCC. This latest finding offers another minimally invasive, cost-effective method for early detection of the disease that can be translated to earlier treatment and potential improvement in long-term survival rates.
Gonzales works in a sprawling space filled with lab equipment and cell lines used in many molecular biology projects. One of her research assistants brought in a cage of lab animals with some strange lumps on their backs.. "These are called nude mice because they dont have a complete immune system," Gonzales explained.. These mice are at the center of a successful experiment. First, scientists used human oral cancer cells to grow large tumors on the animals. They tried one oral cancer drug already on the market. Not much action. Then, they tried a lung cancer drug, also already approved by the Food and Drug Administration. Not that effective on its own. Finally, they used a combination of two drugs. What happened made the medical profession take notice.. "When we combined the two, then we saw a 50 percent reduction in the tumor volumes after 14 days," Gonzales described.. That kind of success could help thousands of patients whose cancers arent caught until the later stage, patients like Paige ...
Oropharynx tumors increased in incidence over the study time period (annual percent change: 1.50% men, 0.8% women), whereas oral cavity tumors decreased (2.10% men, 0.4% women), as did other HNCs (decreased by 3.0% for men and 1.9% for women). The median age at diagnosis for oropharynx cancer decreased by an average of 0.23 years/y. There was no change for oral cavity tumors but an increase for other HNCs of 0.12 years/y. Survival for patients with oropharynx cancer increased by 1.5%/y but was significant for men only. Survival for patients with oral cavity and other HNCs also increased in men only by 0.9%/y and 0.25%/y, respectively.. Conclusion ...