It is unclear if buccal cell samples contain sufficient human DNA with adequately sized fragments for high throughput genetic bioassays. Yet buccal cell sample collection is an attractive alternative to gathering blood samples for genetic epidemiologists engaged in large-scale genetic biomarker studies. We assessed the genotyping efficiency (GE) and genotyping concordance (GC) of buccal cell DNA samples compared to corresponding blood DNA samples, from 32 Nurses Health Study (NHS) participants using the Illumina Infinium 660W-Quad platform. We also assessed how GE and GC accuracy varied as a function of DNA concentration using serial dilutions of buccal DNA samples. Finally we determined the nature and genomic distribution of discordant genotypes in buccal DNA samples. The mean GE of undiluted buccal cell DNA samples was high (99.32%), as was the GC between the paired buccal and blood samples (99.29%). GC between the dilutions versus the undiluted buccal DNA was also very high (greater than ...
It is unclear if buccal cell samples contain sufficient human DNA with adequately sized fragments for high throughput genetic bioassays. Yet buccal cell sample collection is an attractive alternative to gathering blood samples for genetic epidemiologists engaged in large-scale genetic biomarker studies. We assessed the genotyping efficiency (GE) and genotyping concordance (GC) of buccal cell DNA samples compared to corresponding blood DNA samples, from 32 Nurses Health Study (NHS) participants using the Illumina Infinium 660W-Quad platform. We also assessed how GE and GC accuracy varied as a function of DNA concentration using serial dilutions of buccal DNA samples. Finally we determined the nature and genomic distribution of discordant genotypes in buccal DNA samples. The mean GE of undiluted buccal cell DNA samples was high (99.32%), as was the GC between the paired buccal and blood samples (99.29%). GC between the dilutions versus the undiluted buccal DNA was also very high (greater than ...
Purpose: The oral mucosal epithelium shows substantial potential for use in regenerative medicine, including the treatment of ocular surface disease. The rationale for using oral mucosal cells is the possibility of treating bilateral ocular surface stem cell disease without the use of immunosuppression. The present project determines the ideal harvesting site for oral mucosal biopsies and the optimum culture media for these cells.. Methods: Pieces of oral epithelium were used from four locations: buccal mucosa (BM), hard palate (HP), lower lip (LL), and transition zone of the lower lip (TZ) of Sprague-Dawley rats. Explants were grown in four different culture media for six days. The media were 1) RPMI 1640 with 10% heat- inactivated fetal bovine serum (FBS), 2 mM L-glutamine, and 50 IU/mL penicillin-streptomycin; 2) EpiLife with epidermal growth supplement; 3) oral keratinocyte media (OKM) with oral keratinocyte growth factor; and 4) DMEM and Ham 12 (1:1 mixture) supplemented with 10% FBS, 5 ...
TY - JOUR. T1 - EXPRESSION of Cyclooxygenase-1 and cyclooxigenase-2 in normal and pathological human oral mucosa. AU - Leone, Angelo. AU - Gerbino, Aldo. AU - Buscemi, Maria. AU - Burruano, Francesco. AU - Tortorici, Silvia. AU - Mauro, Annamaria. AU - Lipari, Luana. AU - Provenzano, Salvatore. PY - 2010. Y1 - 2010. N2 - Abstract: Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins(PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cellhomeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is stronglyexpressed in inflammation. The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosalreactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positiveoutcome of oral cancer; therefore it would be useful to identify molecular markers ...
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Background: The human microbiota is postulated to affect cancer risk, but collecting microbiota specimens with prospective follow-up for diseases will take time. Buccal cell samples have been obtained from mouthwash for the study of human genomic DNA in many cohort studies. Here, we evaluate the feasibility of using buccal cell samples to examine associations of human microbiota and disease risk.. Methods: We obtained buccal cells from mouthwash in 41 healthy participants using a protocol that is widely employed to obtain buccal cells for the study of human DNA. We compared oral microbiota from buccal cells with that from eight other oral sample types collected by following the protocols of the Human Microbiome Project. Microbiota profiles were determined by sequencing 16S rRNA gene V3-V4 region.. Results: Compared with each of the eight other oral samples, the buccal cell samples had significantly more observed species (P , 0.002) and higher alpha diversity (Shannon index, P , 0.02). The ...
TY - JOUR. T1 - Type IV collagen α6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium. AU - Komori, Taishi. AU - Ono, Mitsuaki. AU - Hara, Emilio satoshi. AU - Ueda, Junji. AU - Nguyen, Ha Thi Thu. AU - Nguyen, Ha Thi. AU - Yonezawa, Tomoko. AU - Maeba, Takahiro. AU - Ono, Aya. AU - Takarada, Takeshi. AU - Momota, Ryusuke. AU - Maekawa, Kenji. AU - Kuboki, Takuo. AU - Oohashi, Toshitaka. PY - 2018/12/1. Y1 - 2018/12/1. N2 - Keratinized mucosa is of fundamental importance to maintain healthy gingival tissue, and understanding the mechanisms of oral mucosa keratinization is crucial to successfully manage healthy gingiva. Previous studies have shown a strong involvement of the basement membrane in the proliferation and differentiation of epithelial cells. Therefore, first, to identify the keratinized mucosa-specific basement membrane components, immunohistochemical analysis for the six alpha chains of type IV collagen was performed in 8-week-old mice. No difference in ...
A significant problem in drug development of novel small molecules is the lack of available tissues (surrogate tissues) that allow for the assessment of the molecular and biochemical effects of (targeted-therapies) drug action. The information obtained from surrogate tissues might help us validate previous preclinical studies with those agents and also dose them in a more rational way. Oral keratinocytes, which are accessible by non-invasive means, might be useful to assess drug action. The proposed study seeks to investigate the genetic, molecular, and biochemical effects of novel agents in oral buccal mucosal cells. Patients already enrolled in Phase I and II clinical trials for neoplastic diseases at the Clinical Center will undergo oral cytobrushing before and during therapy to determine the molecular and biochemical effects of novel agents in the oral mucosa cells. Similar studies will be performed in peripheral blood mononuclear cells. In order to validate to compare and compare the oral ...
The Streptococcus genus comprises ninety-two recognized species that are present in a wide variety of habitats [1]. In humans and animals, a number of streptococcal species are important pathogens (e.g., S. pneumoniae, S. pyogenes, S. suis, and S. mutans), while others are members of mutualistic microflora (e.g., S. oralis, S. downei, S. dentirousetti, and S. salivarius). The species of the Streptococcus genus have been divided into six groups (anginosus, bovis, mitis, mutans, pyogenic, and salivarius) based on 16S rDNA phylogenetic inferences [2]. According to these authors, the salivarius group is composed of three species: (1) S. salivarius, a pioneer colonizer of the human oral mucosa that is isolated mainly from the dorsum of the tongue, the cheeks, and the palate [3], (2) S. vestibularis, a mutualistic bacterium that is present on the vestibulum of the human oral mucosa [4], and (3) S. thermophilus, a thermophilic species [5] that is part of starter cultures used in the production of ...
Purpose: To induce the expression of corneal epithelium-specific cytokeratin 3 (K3) in immortalized human oral mucosal epithelial cells (OKF6/TERT cells) using lentiviral transduction of Pax6.. Methods: OKF6/TERT cells were transduced with two types of lentiviruses, each carrying one of the two variants of Pax6 (Pax6 variant 1 and 2). The cells were cultured in modified keratinocyte serum-free medium (K-sfm) for 3 days after transduction and were cultured in keratinocyte conditioned medium (KCM) with 3T3 feeder cells for another 11 days to stratify them. The gene expressions were examined with quantitative reverse transcription PCR (qRT-PCR) and immunofluorescence imaging on day 3 and day 14.. Results: OKF6/TERT cells had no expression of K3 without transduction of Pax6 (Gene expression compared to GAPDH was 0.01±0.00). OKF6/TERT cells expressed K3 on day 3 only when they were transduced with Pax6 variant 1 (Gene expression compared to GAPDH was 7.31±0.66, P,0.05). Mucin 16 (MUC16) was also ...
Additional file 1: of Transplantation of oral mucosal epithelial cells seeded on decellularized and lyophilized amniotic membrane for the regeneration of injured endometrium
Introduction: Vaginal delivery of medication is advantageous in allowing for the medication to avoid first-pass metabolism and gastrointestinal degrad...
The oral mucosa is the mucous membrane lining the inside of the mouth and consists of stratified squamous epithelium termed oral epithelium and an underlying connective tissue termed lamina propria. The oral cavity has sometimes been described as a mirror that reflects the health of the individual. Changes indicative of disease are seen as alterations in the oral mucosa lining the mouth, which can reveal systemic conditions, such as diabetes or vitamin deficiency, or the local effects of chronic tobacco or alcohol use. Oral mucosa can be divided into three main categories based on function and histology: Masticatory mucosa, keratinized stratified squamous epithelium, found on the dorsum of the tongue, hard palate and attached gingiva. Lining mucosa, nonkeratinized stratified squamous epithelium, found almost everywhere else in the oral cavity, including the: Buccal mucosa refers to the inside lining of the cheeks and floor of the mouth and is part of the lining mucosa. Labial mucosa refers to ...
This page includes the following topics and synonyms: Labial mucosa, Buccal mucosa, Masticatory Mucosa, Movable Mucosa, Oral Mucosa.
Most treatment failure of buccal mucosal cancer post surgery is locoregional recurrence. We tried to figure out how close the surgical margin being unsafe and needed further adjuvant treatment. Between August 2000 and June 2008, a total of 110 patients with buccal mucosa carcinoma (25 with stage I, 31 with stage II, 11 with stage III, and 43 with Stage IV classified according to the American Joint Committee on Cancer 6th edition) were treated with surgery alone (n = 32), surgery plus postoperative radiotherapy (n = 38) or surgery plus adjuvant concurrent chemoradiotherapy (n = 40). Main outcome measures: The primary endpoint was locoregional disease control. The median follow-up time at analysis was 25 months (range, 4-104 months). The 3-year locoregional control rates were significantly different when a 3-mm surgical margin (≤3 versus >3 mm, 71% versus 95%, p = 0.04) but not a 5-mm margin (75% versus 92%, p = 0.22) was used as the cut-off level. We also found a quantitative correlation between
Aim: To evaluate the prevalence of oral mucosa lesions among diabetic patients in, South Kerala, India. Methods: A cross- sectional observational stud..
White lesions are the pathological changes seen in the oral cavity involving the soft tissues like buccal mucosa, palatal mucosa, tongue and floor of mouth.
Methylation status in normal oral epithelial cells and HNSCC.A: RUNX3 expression was examined after 5-aza-2′-deoxycytidine (5-Aza) treatment. HSC4 cells were
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OUTLINE: This is a multicenter study.. Previously procured and archived bone marrow aspirate samples, blood and buccal cell samples, and bone marrow biopsy slides are analyzed for FLT3 ITD, MLL PTD, NPM1, KIT, KRAS, NRAS, CEBPA, WT1, JAK2, RUNX1, TET2, ASXL1, IDH1 and IDH2, and CBL mutations, CBF fusion genes, levels of BAALC, ERG, EVI1, MN1, and APP microarray gene-expression, microRNA gene-expression signature, levels of methylation of genes silenced in AML, and genomic DNA by PCR amplification, RT-PCR, and denaturing high-performance liquid chromatography. ...
Yu G, Phillips S, Gail MH, Goedert JJ, Humphrys M, Ravel J, Ren Y, Caporaso NE. "Evaluation of Buccal Cell Samples for Studies of Oral Microbiota." Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.. 2017 Feb 0; 26(2):249-253. Epub 2016 Oct 21. 1/2017 ...
In 16 patients treated for squamous cell carcinoma of the oral cavity or oropharynx with an accelerated split course regimen, acute mucosal reactions were significantly less in the left buccal mucosa which had been repeatedly painted with 2% silver-n
Nodules or cysts of the oral mucosa occurred with an incidence of 88.7 per cent in 541 Japanese newborn infants. No infant was over 8 days old. This incidence is higher than that reported in Caucasian and Negro newborns. It may be suggested that the
Low Grade Epithelial Dysplasia - Helping folks to collect details about HPV and Cervical Smear to cover with their health care practitioners.
The soft tissue of the mouth is normally lined with mucosa, which is a special type of skin that should appear smooth in texture and pink in color. Any alteration of the color or texture of the mucosa (lesion) may signal the beginning of a pathologic process. These changes may occur on the face, neck, and areas of the mouth (e.g., gums, tongue, lips, etc.). The most serious of these pathologic changes (which may or may not be painful) is oral cancer. The following can be signs of a suspicious pathologic process such as oral cancer: ...
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Whitish, shredded appearance of the buccal or labial mucosa at the occlusal line caused by biting. The lesions are benign. The habit is most common in tense or anxious individuals.. ...
This is the official approximate match mapping between ICD9 and ICD10, as provided by the General Equivalency mapping crosswalk. This means that while there is no exact mapping between this ICD10 code K13.79 and a single ICD9 code, 528.9 is an approximate match for comparison and conversion purposes. ...
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博士(医学) 乙第2859号, 著者名:Mikiko Fujita・Yuri Nakamura・Saeko Kasashima・Maiko Furukawa・Ryoichi Misaka・Hikaru Nagahara,タイトル:Risk
D. Moraitis, B. Du, M De Lorenzo, J.O. Boyle, B.B. Weksler, E.G. Cohen, J.F. Carew, N.K. Altorki, L. Kopelovich, K. Subbaramaiah, A.J. Dannenberg. Levels of COX-2 are increased in the oral mucosa of smokers. Evidence for the role of EGFR and its ligands. Cancer Res, 2005, 65(2): 1- ...
Semantic Scholar extracted view of The influence of some antifungal drugs on in vitro adherence of Candida albicans to human buccal epithelial cells. by Anna Barbara Macura
Mucosal epithelial surfaces, such as line the oral cavity, are common sites of microbial colonization by bacteria, yeast and fungi. The microbial interactions involve adherence between the glycans on the host cells and the carbohydrate-binding proteins of the pathogen. Saliva constantly bathes the buccal cells of the epithelial surface of the mouth and we postulate that the sugars on the salivary glycoproteins provide an innate host immune mechanism against infection by competitively inhibiting pathogen binding to the cell membranes. The structures of the N- and O-linked oligosaccharides on the glycoproteins of saliva and buccal cell membranes were analyzed using capillary carbon liquid chromatography-electrospray ionization MS/MS. The 190 glycan structures that were characterized were qualitatively similar, but differed quantitatively, between saliva and epithelial buccal cell membrane proteins. The similar relative abundance of the terminal glycan epitope structures (e.g. ABO(H) blood group, ...
BI-D1870 is a potent and specific RSK inhibitor (the p90 ribosomal S6 kinase), which inhibits RSK1, RSK2, RSK3 and RSK4 in vitro with an IC50 of 10Â-30 nM. BI-D1870 exhibited a dose-responsive antiproliferative effect on OSCC cells with relative sparing of normal human oral keratinocytes. The compound inhibited the downstream RSK target YB-1 and caused apoptosis. In addition, BI-D1870 also induced G2/M arrest by modulating the expression of p21 and other cell cycle regulators. BI-D1870 may be of useful in oral squamous cell carcinoma therapy.
Spinal cord injury, involving damaged axons and glial scar tissue, often culminates in irreversible impairments. Achieving substantial recovery following complete spinal cord transection remains an unmet challenge. Here, we report of implantation of an engineered 3D construct embedded with human oral mucosa stem cells (hOMSC) induced to secrete neuroprotective, immunomodulatory and axonal elongation-associated factors, in a complete spinal cord transection rat model. Rats implanted with induced tissue engineering constructs regained fine motor control, coordination and walking pattern in sharp contrast to the untreated group that remained paralyzed (42% vs. 0%). Immunofluorescence, CLARITY, MRI and electrophysiological assessments demonstrated a reconnection bridging the injured area, as well as presence of increased number of myelinated axons, neural precursors, and reduced glial scar tissue in recovered animals treated with the induced cell-embedded constructs. Finally, this construct is made of bio
Remember that surface lesions of oral mucosa consist of lesions that involve the epithelium and/ or superficial connective tissue. They do not exceed 2-3 mm in thickness. Clinically, surface lesions are flat or slightly thickened rather than being swellings or enlargements.. We initially divide surface lesions into three categories based on their clinical appearance: white, pigmented, and vesicular-ulcerated-erythematous.. White Surface Lesions of Oral Mucosa. Surface lesions of oral mucosa that appear white, tan, or light yellow are divided into three groups based on their clinical features:. 1. White lesions due to epithelial thickening. 2. White lesions due to accumulation of necrotic debris on the mucosal surface. 3. White lesions due to subepithelial changes in the connective tissue.. Epithelial thickening white lesions appear white because the pink to red color of the blood vessels in the underlying connective tissue is masked by the increased thickness of the epithelium. These lesions are ...
Understanding how optical properties are altered during oral carcinogenesis is critical for optimizing diagnostic technologies for oral cancer detection based on autofluorescence imaging and spectroscopy. In this study, we used high-resolution microscopy to investigate patterns of autofluorescence in normal oral mucosa and in benign and neoplastic oral lesions. Our results show that the autofluorescence properties of oral tissue vary based on the anatomic site within the oral cavity and the pathologic diagnosis. The fluorescence signals from epithelial and stromal layers can change independently of other tissue layers. This has important implications for the clinical diagnosis of oral lesions using fluorescence imaging and spectroscopy.. When normal oral tissue is illuminated by UV light, most of the epithelial autofluorescence that is generated originates from the cytoplasm of cells occupying the basal and intermediate layers. Similar findings were found using confocal images of cervical ...
Geographic tongue is quite common benign condition that primarily affects tongue and rarely other oral mucous membranes (lips, cheeks, palate, gums). There may be more red lesions on the tongue, which are well delimited and surrounded by a slightly raised white border. Some of the lesions can also shed. They persist in one place for some time and then completely disappear and appear elsewhere. They can be present for weeks, months or years. There is no pain present. Together with geographic lesion there can also be fissures (fissured tongue). This condition can occur at any age, more commonly in women. It may also be linked with a skin disease psoriasis. No treatment is necessary. Read more ...
Squamous cell carcinoma (SCC) is the most common form of oral malignancy and is often preceded by premalignant lesions, some of which are more likely to progress to carcinoma than others. In this study, a panel of monoclonal antibodies (AE1/AE3, cytokeratin [CK] 14, Ki-67 and p53) is applied to 10 cases of human oral tissue in each of six categories to establish staining patterns indicative of which lesions are more likely to progress to malignancy. The six tissue categories are normal tissue; abnormal benign lesions; mild, moderate and severe dysplasia; and SCC. A statistical analysis of Ki-67 and p53 immunoexpression is performed. The results showed that AE1/AE3 and CK 14 expression was reduced as a late event in oral carcinogenesis, particularly in poorly differentiated SCC. Expression of Ki-67 and p53 proved to be a weak but statistically significant predictor of malignant progression in oral tissue.
Detailed drug Information for acyclovir Buccal mucosa. Includes common brand names, drug descriptions, warnings, side effects and dosing information.
The human 5T4 oncofoetal antigen is expressed by all types of trophoblast in pregnancy but is not detected on most adult tissues, although low levels are found on some epithelia. However, this antigen is strongly expressed by many cancers and tumour-associated labelling correlates with metastatic spread and poor clinical outcome for patients with gastric and colon cancer. Over-expression of the gene influences cell adhesion, shape and motility, which may be related to changes in the cellular localisation of the 5T4 oncofoetal antigen as malignancy develops. To establish whether the 5T4 oncofoetal antigen can serve as a tumour-specific marker for oral cancer and precancer, we have evaluated the pattern of expression on biopsies of normal, inflamed and dysplastic oral mucosa using immunohistochemistry. Oral mucosa, taken from different sites in the mouth, expressed the 5T4 oncofoetal antigen with varying intensity and pattern. The majority of the immunoreactivity was detected in the basal and ...
(a) Epithelial dysplasia with basement membrane intact from a biopsy taken from leukoplakia on (H and E, ×100), (b) epithelial dysplasia on (H and E, ×400)
Obtaining high quality genomic DNA is critical for epidemiological studies that aim to evaluate the role of genetic factors in human disease susceptibility. Blood samples are an excellent source of large amounts of genomic DNA. However, epidemiological studies often need alternative sources when study subjects are reluctant to provide a blood sample, when only a self-administered collection protocol is logistically or economically feasible, or as a back-up source of DNA in studies that collect blood samples. Exfoliated buccal epithelial cells and other cells found in saliva are a very promising alternative source of DNA because they can be obtained using self-administered, noninvasive, and relatively inexpensive techniques (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12) . Buccal swabs and mouthwash protocols are the most commonly used protocols for buccal cell collection. Studies using different types of buccal swabs, i.e., cotton swabs or cytobrushes, have obtained similar DNA yields and PCR success ...
TY - JOUR. T1 - Perfusion cells for studying regional variation in oral mucosal permeability in humans. 2. A specialized transport mechanism in D-glucose absorption exists in dorsum of tongue. AU - Kurosaki, Yuji. AU - Yano, Koji. AU - Kimura, Toshikiro. PY - 1998/5. Y1 - 1998/5. N2 - To clarify the site of D-glucose absorption in human oral cavity, newly designed perfusion cells were applied to five different sites in the human oral cavity, i.e., the dorsum of the tongue, the ventral surface of the tongue, the labial mucosa, the floor of the mouth, and the buccal mucosa. The solution of D-glucose was perfused for 1 h and the rate of absorption was calculated from the amount that disappeared from the perfusate. D-Glucose was absorbed rapidly from the dorsum of the tongue and the absorption was saturable. The saturable absorption was also observed in the ventral surface of the tongue, but not in the other three sites. The rate of D-glucose absorption in the dorsum and the ventral surface of the ...
In: 5th L.H. Gray Trust Workshop, The single cell microgel assay (COMET), technical aspects and applications, Sutton, England 1994. London : Inst. of Cancer Res. 1994 ...
Bekijk Stockfoto van Stratified Squamous Epithelium From The Human Mouth Mucosa He Stain Lm X100. Ga voor hoogwaardige fotos met een hoge resolutie naar Getty Images.
Expression of CD5L (API6, Spalpha) in oral mucosa tissue. Antibody staining with HPA065686 and HPA068384 in immunohistochemistry.
Expression of ADAM10 (CD156c, HsT18717, kuz, MADM) in oral mucosa tissue. Antibody staining with CAB001709 in immunohistochemistry.
Objectives: The oral mucosa is a susceptible component of our anatomy with explicit implications on our well being. Consequently an unfamiliar pattern of oral mucosa pathology will raise much concern. Confronting unrecognized lesions undoubtedly challenges our professional acumen and clinical expertise. In attempt to discern the gravity of an exceptional condition the caring dermatologist may contemplate to indulge any of a number of approaches with different levels of intervention. This paper describes the rationale for choosing a conservative clinical management approach to two children exhibiting unique oral lesions. Methods: Two youngsters arriving for consultation, presented approximately twelve months apart with similar oral lesions. They were examined and thorough case histories were taken. The findings were photographed, a bacterial culture was taken and follow up ensued. Results: Both youngsters displayed pseudomembranous plaques covering a limited zone of the oral mucosa. One case involved the
Oral tissue biopsies in Bloomingdale are necessary for lesions that cannot be diagnosed by clinical findings. Biopsies provide tissue for microscopic analysis.