We have previously described the severe multi-organ developmental defects in the B6;129P2-Tmem67tm1Dgen/H knockout mouse that reiterate the clinical features of MKS and JBTS (Abdelhamed et al., 2013). All Tmem67−/− mutants that were examined, developed incomplete laterality defects that manifested in late gestation as left lung isomerism (Fig. 1A) and were occasionally associated with dextrocardia (Fig. 1E,F). Pulmonary hypoplasia was a consistent finding in the Tmem67−/− embryos and pups (Fig. 1A,B), although this is frequently under-reported in human ciliopathies and not considered an essential diagnostic clinical feature of MKS in humans (Salonen, 1984). However, it has been reported recently that, for MKS, death occurs in utero or within hours after birth because of the pulmonary hypoplasia, which can be considered as the leading cause of death in human MKS patients (Roy and Pal, 2013).. Previously, we have shown that TMEM67 is required for epithelial branching morphogenesis in ...
Axial pattern flaps are pedicle grafts which incorporate a direct cutaneous artery and vein at their base. While not a true axial pattern flap, branches of the saphenous artery are the direct cutaneous artery for the reverse saphenous conduit axial pattern flap. The direct cutaneous artery and vein extend along the length of the flap for a variable distance and the terminal branches supply the subdermal, cutaneous, and subpapillary plexuses. The reverse saphenous conduit axial pattern flap is indicated for reconstruction of wounds of the distal pelvic limb.. In a 2015 study investigating the outcome of axial pattern flaps in 49 dogs and 24 cats, postoperative complications were reported in 89% of animals. The most common complications included dehiscence (50% of axial pattern flaps in dogs and 75% of axial pattern flaps in cats), flap swelling (43% of axial pattern flaps in dogs and 50% of axial pattern flaps in cats), necrosis (46% of axial pattern flaps in dogs and 15% of axial pattern flaps ...
Epithelial branching morphogenesis drives the development of organs such as the lung, salivary gland, kidney and the mammary gland. It involves cell proliferation, cell differentiation and cell migration. An elaborate network of chemical and mechanical signals between the epithelium and the surrounding mesenchymal tissues regulates the formation and growth of branching organs. Surprisingly, when cultured in isolation from mesenchymal tissues, many epithelial tissues retain the ability to exhibit branching morphogenesis even in the absence of proliferation. In this work, we propose a simple, experimentally plausible mechanism that can drive branching morphogenesis in the absence of proliferation and cross-talk with the surrounding mesenchymal tissue. The assumptions of our mathematical model derive from in vitro observations of the behaviour of mammary epithelial cells. These data show that autocrine secretion of the growth factor TGFβ1 inhibits the formation of cell protrusions, leading to ...
Axial pattern flaps are pedicle grafts which incorporate a direct cutaneous artery and vein at their base. The cutaneous branch of the thoracodorsal artery is the direct cutaneous artery for the thoracodorsal axial pattern flap. The direct cutaneous artery and vein extend along the length of the flap for a variable distance and the terminal branches supply the subdermal, cutaneous, and subpapillary plexuses. The thoracodorsal axial pattern flap is indicated for reconstruction of wounds of the ipsilateral thoracic limb and thoracic wall.. In a 2015 study investigating the outcome of axial pattern flaps in 49 dogs and 24 cats, postoperative complications were reported in 89% of animals. The most common complications included dehiscence (50% of axial pattern flaps in dogs and 75% of axial pattern flaps in cats), flap swelling (43% of axial pattern flaps in dogs and 50% of axial pattern flaps in cats), necrosis (46% of axial pattern flaps in dogs and 15% of axial pattern flaps in cats), infection ...
Ming, C. H., Wasserman, D., Hartwig, S., & Osenblum, N. D. (2004). P38MAPK Acts in the BMP7-dependent stimulatory pathway during Epithelial Cell Morphogenesis and is regulated by Smadl1. Journal Of Biological Chemistry, 279(13), 12051-12059. doi:10.1074/jbc. ...
The developmental activities of morphogens depend on the gradients that they form in the extracellular matrix. Here, we show that differences in the binding of fibroblast growth factor 7 (FGF7) and FGF10 to heparan sulfate (HS) underlie the formation of different gradients that dictate distinct activities during branching morphogenesis. Reducing the binding affinity of FGF10 for HS by mutating a single residue in its HS-binding pocket converted FGF10 into a functional mimic of FGF7 with respect to gradient formation and regulation of branching morphogenesis. In particular, the mutant form of FGF10 caused lacrimal and salivary gland epithelium buds to branch rather than to elongate. In contrast, mutations that reduced the affinity of the FGF10 for its receptor affected the extent, but not the nature, of the response. Our data may provide a general model for understanding how binding to HS regulates other morphogenetic gradients.. ...
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The oligomeric IκB kinase (IKK) is composed of three polypeptides: IKKα and IKKβ, the catalytic subunits, and IKKγ, a regulatory subunit. IKKα and IKKβ are similar in structure and thought to have similar function-phosphorylation of the IκB inhibitors in response to proinflammatory stimuli. Such phosphorylation leads to degradation of IκB and activation of nuclear factor κB transcription factors. The physiological function of these protein kinases was explored by analysis of IKKα-deficient mice. IKKα was not required for activation of IKK and degradation of IκB by proinflammatory stimuli. Instead, loss of IKKα interfered with multiple morphogenetic events, including limb and skeletal patterning and proliferation and differentiation of epidermal keratinocytes. ...
The increasing repertoire of microRNAs expressed during organ development and their role in regulating organ morphogenesis provide a compelling need to develop methods to assess microRNA function using various in vitro and in vivo experimental models
Antibodies for proteins involved in post-embryonic organ morphogenesis pathways, according to their Panther/Gene Ontology Classification
Large wounds that do not allow primary closure require the use of other reconstruction techniques. Axial pattern flaps are local skin flaps that include a direct cutaneous artery and vein in their base. These enable us to transfer a large section of skin into an adjacent defect, providing viable tissue to cover an extensive wound.. This retrospective study gives a detailed account of how a lateral caudal axial pattern flap was used to close large skin defects in the dorsum, gluteal, and perineal area of 13 dogs. Eleven dogs required reconstruction after tumor removal and two of them had skin defects due to trauma.. Four dogs presented short term complications following surgery. Two of them required additional care, one for mild dehiscence and the other one for distal flap necrosis. These were considered minor postoperative complications. The two remaining dogs presented the same complications, but in their particular case, a new surgery was needed to achieve definitive closure. The authors ...
This article investigates the evolutionary dynamics of morphogenesis. In this study, morphogenesis arises as a side-effect of maximization of number of cell types. Thus, it investigates the evolutionary dynamics of side-effects. Morphogenesis is governed by the interplay between differential cell adhesion, gene-regulation, and intercellular signaling. Thus, it investigates the potential to generate complex behavior by entanglement of relatively boring processes, and the (automatic) coordination between these processes.. The evolutionary dynamics shows all the hallmarks of evolutionary dynamics governed by nonlinear genotype phenotype mapping: for example, punctuated equilibria and diffusion on neutral paths. More striking is the result that interesting, complex morphogenesis occurs mainly in the shadow of neutral paths which preserve cell differentiation, that is, the interesting morphologies arise as mutants of the fittest individuals.. Characteristics of the evolution of such side-effects ...
The past decades have seen a rapid increase in the understanding of plant morphogenesis at the molecular-genetic level. However, the control of growth and morphogenesis by molecular and signaling networks ultimately requires the coordinated regulation of mechanical properties in individual cells. Th …
The cellular changes constituting morphogenesis are executed by structural molecules involved with adhesion and cytoskeletal structure. The actin based cytoskeleton, rho/racGTPases, as well as the cadherin-catenin complex have been implicated in epithelial folding and convergent-extension (Sullivan and Theurkauf, 1995; Lu and Settleman, 1998; Tepass, 1999), although a detailed model placing functional interconnections between the different molecules has not yet materialized. The present paper demonstrates that a finely adjusted level of DE-cadherin is required for optic placode morphogenesis, and that β-catenin, as well as EGFR signaling, is involved in this process. Reduction in DE-cadherin results in dissociation of the placode around the time when it normally invaginates, suggesting that the forces exerted on the epithelial sheet while folding may disrupt cell contacts. A similar phenotype was described for other epithelial invaginations, including the Malpighian tubules and stomatogastric ...
Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis.: Several E-box-binding transcription factors regulate individual and collective cell
Plant morphogenesis and its regulation have fascinated researchers for more than two centuries. Among determinants of morphogenesis mechanical signals appear as an important cue. The fact that plants respond to mechanical stimuli was reported by Darwin in the 1850s. As described by Iida in this research topic, mechanical stimuli were used in traditional agriculture practices like mugifumi. In the past 40 years, the study of mechanical signaling in plants has regained interest because of its implication in fundamental processes of organo- and morphogenesis and their potential as an innovative means of controlling plant growth. The focus of this research topic is the quantification of mechanical signals and of their effects on plant growth, the ecological significance of mechanoperception and thigmomorphogenesis. The papers in this research topic summarize the current state of knowledge, present new experimental results, identify areas where further investigation is warranted, and propose investigative
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Research groupsGene regulation and morphogenesis Control of epithelial morphogenesis in vertebrates Dr Juan Ramón Martínez ..
The CXB set of recombinant inbred mouse strains provided an opportunity to observe the effects of reassorted subsets of genes on the shape of the mandible. The distances between 12 landmarks in all paired combinations were calculated to evaluate genetic control in small regions. The genetic relationships between interlandmark distances revealed genes to have most of their effects in localized regions, and the greater heritabilities usually to apply to those distances between adjacent landmarks. Interrelationships between measurements are usually explicable on a developmental basis. It is proposed that genes of this sort bring about the changes seen in organ shape during evolution. A model plan for the organization of gene activation during morphogenesis is described.
The roles of cellular orientation during trabecular and ventricular wall morphogenesis are unknown and so are the underlying mechanisms that regulate cellular orientation. Myocardial-specific Numb and Numblike double-knockout (MDKO) hearts display a variety of defects including in cellular orientation patterns of mitotic spindle orientation trabeculation and ventricular compaction. Furthermore Numb- and Numblike-null cardiomyocytes exhibit cellular behaviors distinct from those of control cells during trabecular morphogenesis based on single-cell lineage tracing. We investigated how Numb regulates cellular orientation and behaviors and determined that N-cadherin levels and membrane localization are reduced in MDKO hearts. To determine how Numb regulates N-cadherin membrane localization we generated an mCherry:Numb knockin line and found that Numb localized to diverse endocytic organelles but mainly to the recycling endosome. Consistent with this localization cardiomyocytes in MDKO did not ...
The morphogenesis of epithelial tissues and organs is profoundly dependent on the extracellular matrix (ECM), especially the specialized forms of ECM known as basement membranes (BMs) (Miner and Yurchenco, 2004). Active remodeling of BMs by tissues is essential for many developmental events, and aberrant cell-ECM interactions underlie tumorigenesis and metastasis of epithelial tissues (Larsen et al., 2006). Developing axons often grow over BM substrates, and BM components play central roles both in tissue morphogenesis and in axon guidance (Hinck, 2004). Yet, rather than a passive scaffold for tissue morphogenesis, the ECM can be regarded as an active participant in tissue morphogenesis and cell signaling (Nelson and Bissell, 2006).. C. elegans embryonic epidermal morphogenesis is an example of an organogenesis process that involves multiple interactions between an epithelial sheet, underlying muscle, and an intervening BM (Chisholm and Hardin, 2005). In late embryogenesis, epidermal cells ...
The overall objective of the four projects in this program of research is to develop and exploit biosensors and image analysis techniques to delineate the mecha...
Background: Increasing the complexity of in vitro systems to mimic three-dimensional tissues and the cellular interactions within them will increase the reliability of data that were previously collected with in vitro systems. In vivo vascularization is based on complex and clearly defined cell-matrix and cell-cell interactions, where the extracellular matrix (ECM) seems to play a very important role. The aim of this study was to monitor and visualize the subcellular and molecular interactions between endothelial cells (ECs), fibroblasts, and their surrounding microenvironment during vascular morphogenesis in a three-dimensional coculture model. Methods: Quantitative and qualitative analyses during the generation of a coculture tissue construct consisting of endothelial cells and fibroblasts were done using transmission electron microscopy and immunohistochemistry. Results: Dynamic interactions were found in cocultures between ECs, between fibroblasts (FBs), between ECs and FBs, and between the cells
Systemic steroid hormone and intracellular signaling pathways are known to act cooperatively during the development of vertebrate and invertebrate epithelia. However, the mechanism of this interaction is poorly understood. Morphogenesis of Drosophila leg imaginal disc epithelia is regulated both by the steroid hormone 20-hydroxyecdysone (ecdysone) and the RhoA GTPase signaling pathway. Recent evidence suggests that these pathways act cooperatively to control imaginal disc morphogenesis. Thus, leg imaginal disc morphogenesis is an excellent system in which to study the interaction of steroid hormone and intracellular signaling pathways. We have identified mutations in three genes, 12-5, 18-5, and 31-6, with roles in the morphogenesis of leg epithelia. Of particular interest, these mutations interact genetically with each other, mutations in the RhoA signaling pathway, and the ecdysone regulated Sb-sbd (Stubble) transmembrane serine protease. This suggests that the 12-5, 18-5, and 31-6 gene products may
TY - UNPB. T1 - Artificial Plants. T2 - Vascular Morphogenesis Controller-guided growth of braided structures. AU - Hofstadler, Daniel Nicolas. AU - Varughese, Joshua Cherian. AU - Nielsen, Stig Anton. AU - Leon, David Andres. AU - Ayres, Phil. AU - Zahadat, Payam. AU - Schmickl, Thomas. PY - 2018/4/17. Y1 - 2018/4/17. N2 - Natural plants are exemplars of adaptation through self-organisation and collective decision making. As such, they provide a rich source of inspiration for adaptive mechanisms in artificial systems. Plant growth-a structure development mechanism of continuous material accumulation that expresses encoded morphological features through environmental interactions-has been extensively explored in-silico. However, ex-silico scalable morphological adaptation through material accumulation remains an open challenge. In this paper, we present a novel type of biologically inspired modularity, and an approach to artificial growth that combines the benefits of material continuity through ...
Kaplan N.A., Colosimo P.F., Liu X., Tolwinski N.S. (2011). Complex interactions between GSK3 and aPKC in drosophila embryonic epithelial morphogenesis. PLoS ONE 6 (4) : e18616. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone. ...
We propose to combine computational and experimental approaches to investigate the mechanisms of epithelial morphogenesis, defined as the set of processes that...
Branching morphogenesis, the creation of branched structures in the body, is a key feature of animal and plant development. This book brings together, for the first time, expert researchers working on
TY - JOUR. T1 - Expression of Spred and Sprouty in developing rat lung. AU - Hashimoto, Shuichi. AU - Nakano, Hiroshi. AU - Singh, Gurmukh. AU - Katyal, Sikandar. N1 - Funding Information: This work was supported by a grant from the National Institutes of Health (HL 62397). We thank Dr. A. Paula Monaghan-Nichols for advice.. PY - 2002/12. Y1 - 2002/12. N2 - Sproutys and Sprouty-related proteins, Spred-1 and -2, are known inhibitors of fibroblast growth factor (FGF) signaling, which plays key role in lung branching morphogenesis and the development of other tissues. The present study demonstrates that Spreds are expressed in a variety of rat embryonic tissues (brain, intestine, heart, skin) including the lung. In the embryonic lung, Spreds and Sproutys are expressed during the early stages of branching morphogenesis, but their expression profiles are both distinct and oveflapping. Spreds are predominantly expressed in mesenchymal cells in contrast to Sproutys, which are abundantly expressed in ...
Four of the mutants examined (tpm1Δ, sac6Δ, pfy1-111, and myo2-66) displayed a clear reduction in viability when the morphogenesis checkpoint was crippled by elimination of Swe1p (Fig. 2 B). This strongly suggests that the actin perturbations caused by the mutants triggered the checkpoint response, as confirmed below.. Intriguingly, the degree of growth benefit provided by Swe1p varied depending on the growth temperature, in a mutant-specific manner. The difference between the growth of different mutants in combination with swe1Δ was most extreme at the temperatures shown in Fig. 2 B but was often reduced at other (7°C higher or lower) temperatures. In the most dramatic example, growth of myo2-66 swe1Δ cells was impaired relative to myo2-66 cells at 29°C, but not at 28°C (Fig. 2 B). This was unexpected because the strain grows slowly and has impaired actin organization at both temperatures. One problem in interpreting growth assays for very sick strains is the accumulation of suppressor ...
This book, the last volume in the Social Morphogenesis series, examines whether or not a Morphogenic society can foster new modes of human relations that could exercise a form of relational steering, protecting and promoting a nuanced version of the good life for all. It analyses the way in which the intensification of morphogenesis and…
Cell morphogenesis is the development of a specific cell morphology, which includes the acquisition of cell shape, cell polarity, cell-cell and cell-ECM (extracellular matrix) contacts
J:173573 Haara O, Fujimori S, Schmidt-Ullrich R, Hartmann C, Thesleff I, Mikkola ML, Ectodysplasin and Wnt pathways are required for salivary gland branching morphogenesis. Development. 2011 Jul;138(13):2681-91 ...
J:152818 Melnick M, Phair RD, Lapidot SA, Jaskoll T, Salivary gland branching morphogenesis: a quantitative systems analysis of the Eda/Edar/NFkappaB paradigm. BMC Dev Biol. 2009;9:32 ...
Invasive growth is a complex morphogenetic program in which proliferative responses are integrated by apparently independent events such as migration, survival, matrix degradation, and induction of cell polarity. In the first step of this sequence (Figure 1), a cell within a colony or solid tissue is instructed to disrupt cadherin-based intercellular junctions and acquire a fibroblastoid, motile phenotype, initiating detachment from the primary site of accretion. This dramatic reshaping is accompanied by cytoskeletal rearrangements and enhanced production of matrix proteases, which digest basal lamina components and facilitate cell movement through the surrounding environment. During this phase, invading cells must induce a constant and dynamic remodeling of integrin-mediated adhesive contacts with the ECM, which provides a mechanical support for cell migration and prevents the induction of apoptosis. Cell depolarization and invasion are followed by stimulation of cell growth, which allows new regions
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in ...
The role of WNT signaling and its interactions with other morphogenetic pathways were investigated during lung development. Previously, we showed that targeted disruption of Wnt5a results in over-branching of the epithelium and thickening of the interstitium in embryonic lungs. In this study, we gen …
Crumbs (Crb) is a conserved determinant of apical membrane identity that regulates epithelial morphogenesis in many developmental contexts. In this study, we
MPATH:458] normal [MPATH:1] cell and tissue damage [MPATH:2] cell death [MPATH:4] necrosis [MPATH:14] degenerative change [MPATH:25] tissue specific degenerative process [MPATH:33] intracellular and extracellular accumulation [MPATH:47] intracellular and extracellular depletion [MPATH:55] developmental and structural abnormality [MPATH:57] agenesis [MPATH:58] aplasia [MPATH:59] branching morphogenesis defect [MPATH:60] communication defect [MPATH:64] dysplasia [MPATH:72] growth acceleration [MPATH:73] growth arrest [MPATH:82] persistent embryonic structure [MPATH:86] organ specific developmental defect [MPATH:107] congestion [MPATH:119] hemorrhage and non-specified extravasation [MPATH:125] thrombosis [MPATH:126] growth and differentiation defect [MPATH:127] atrophy [MPATH:133] hypoplasia [MPATH:134] hyperplasia [MPATH:159] hypertrophy [MPATH:160] metaplasia [MPATH:175] healing and repair [MPATH:176] connective tissue replacement [MPATH:179] fibrin deposition [MPATH:180] fibroblast proliferation ...
MPATH:458] normal [MPATH:1] cell and tissue damage [MPATH:2] cell death [MPATH:4] necrosis [MPATH:14] degenerative change [MPATH:25] tissue specific degenerative process [MPATH:33] intracellular and extracellular accumulation [MPATH:47] intracellular and extracellular depletion [MPATH:55] developmental and structural abnormality [MPATH:57] agenesis [MPATH:58] aplasia [MPATH:59] branching morphogenesis defect [MPATH:60] communication defect [MPATH:64] dysplasia [MPATH:72] growth acceleration [MPATH:73] growth arrest [MPATH:82] persistent embryonic structure [MPATH:86] organ specific developmental defect [MPATH:107] congestion [MPATH:119] hemorrhage and non-specified extravasation [MPATH:125] thrombosis [MPATH:126] growth and differentiation defect [MPATH:127] atrophy [MPATH:133] hypoplasia [MPATH:134] hyperplasia [MPATH:159] hypertrophy [MPATH:160] metaplasia [MPATH:175] healing and repair [MPATH:176] connective tissue replacement [MPATH:179] fibrin deposition [MPATH:180] fibroblast proliferation ...
Plays a critical role in epithelial cell morphogenesis, polarity, adhesion and cytoskeletal organization in the lens (PubMed:26231217).
View Notes - bicd130_06_lecture9r from BICD 130 at UCSD. BICD 130 Embryos, Genes, and Development MORPHOGENESIS Establishment of form and position in the developing organism: Rearrangement of cell
Although in previous chapters in some cases we have attempted a theoretical analysis of some aspects of morphogenesis, the main purpose of these chapters was to describe the up-to-date factual...
This book series Cardiovascular Molecular Morphogenesis publishes works devoted to the development of the heart and blood vessels. Since both the developing embryo vessels and adult blood vessels are of great current interest, such ...
Morphogenesis of Endothelium von Gabor M. Rubanyi und Buchbewertungen gibt es auf ReadRate.com. Bücher können hier direkt online erworben werden.
A lecture course on morphogenesis for fourth-year Moscow State University Specialist Diploma students specializing in embryology is described. The main goal of the course is to give the students an extensive theoretical background based on the tenets of the modern theory of Self-Organization and to show them how important this theory is for the proper understanding of developmental events. The corresponding mathematics are bound as tightly as possible to the actual morphogenetic processes. All of the lectures take the format of an active dialogue between the students and a tutor.
It has long been suggested that the generation of biological patterns depends in part on gradients of diffusible substances. In an attempt to bridge the gap between this largely theoretical concept and experimental embryology, we have examined the physiology of diffusion gradients in an actual embryonic field. In particular, we have generated in the chick wing bud concentration gradients of the morphogenetically active retinoid TTNPB, (E)-4-[2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-prope nyl] benzoic acid, a synthetic vitamin A compound. Upon local application of TTNPB the normal 234 digit pattern is duplicated in a way that correlates with the geometry of the underlying TTNPB gradient; low doses of TTNPB lead to a shallow gradient and an additional digit 2, whereas higher doses result in a steep, far-reaching gradient and patterns with additional digits 3 and 4. The experimentally measured TTNPB distribution along the anteroposterior axis, can be modeled by a local source and ...
GO:0032835 glomerulus development --, regulation of glomerulus development GO:NEW --,negative regulation of glomerulus development GO:NEW --,positive regulation of glomerulus development GO:NEW Also as an extension to SF#2912058 requested 10/12/09 GO:0001658 branching involved in ureteric bud morphogenesis --,regulation of branching involved in ureteric bud morphogenesis GO:NEW --,negative regulation of branching involved in ureteric bud morphogenesis GO:NEW --,positive regulation of branching involved in ureteric bud morphogenesis GO:NEW SF 2914121 [YAF 17/12/09] David Added: regulation of branching involved in ureteric bud morphogenesis ; GO:0090189/ positive regulation of branching involved in ureteric bud morphogenesis ; GO:0090190/ negative regulation of branching involved in ureteric bud morphogenesis ; GO:0090191 regulation of glomerulus development ; GO:0090192/ positive regulation of glomerulus development ; GO:0090193/ negative regulation of glomerulus development ; GO:0090194. ...
GO:0032835 glomerulus development --, regulation of glomerulus development GO:NEW --,negative regulation of glomerulus development GO:NEW --,positive regulation of glomerulus development GO:NEW Also as an extension to SF#2912058 requested 10/12/09 GO:0001658 branching involved in ureteric bud morphogenesis --,regulation of branching involved in ureteric bud morphogenesis GO:NEW --,negative regulation of branching involved in ureteric bud morphogenesis GO:NEW --,positive regulation of branching involved in ureteric bud morphogenesis GO:NEW SF 2914121 [YAF 17/12/09] David Added: regulation of branching involved in ureteric bud morphogenesis ; GO:0090189/ positive regulation of branching involved in ureteric bud morphogenesis ; GO:0090190/ negative regulation of branching involved in ureteric bud morphogenesis ; GO:0090191 regulation of glomerulus development ; GO:0090192/ positive regulation of glomerulus development ; GO:0090193/ negative regulation of glomerulus development ; GO:0090194. ...
TY - JOUR. T1 - Quantification of gene expression patterns to reveal the origins of abnormal morphogenesis. AU - Martínez-Abadías, Neus. AU - Estivill, Roger Mateu. AU - Tomas, Jaume Sastre. AU - Perrine, Susan Motch. AU - Yoon, Melissa. AU - Robert-Moreno, Alexandre. AU - Swoger, Jim. AU - Russo, Lucia. AU - Kawasaki, Kazuhiko. AU - Richtsmeier, Joan. AU - Sharpe, James. PY - 2018/9. Y1 - 2018/9. N2 - The earliest developmental origins of dysmorphologies are poorly understood in many congenital diseases. They often remain elusive because the first signs of genetic misregulation may initiate as subtle changes in gene expression, which are hard to detect and can be obscured later in development by secondary effects. Here, we develop a method to trace back the origins of phenotypic abnormalities by accurately quantifying the 3D spatial distribution of gene expression domains in developing organs. By applying Geometric Morphometrics to 3D gene expression data obtained by Optical Projection ...
The morphogenetic behavior of a tropical marine Yarrowia lipolytica strain on hydrophobic substrates was studied. Media containing coconut oil or palm kernel oil (rich in lauric and myristic acids) prepared in distilled water or seawater at a neutral
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The publication of `The Chemical Basis of Morphogenesis by ALAN M. TURING in 1952 was a milestone for the development of mathematical biology and for many (biological) disciplines leaning on it. TURING provided an original solution to the problem of morphogenesis, by adapting a system of coupled differential equations to describe both chemical reaction and diffusion of morphogenetic substances in an initially homogeneous configuration. FOURIERS analysis of the `ring problem in heat conduction, and the theory of spherical harmonies and their solution by (normalized) LEGENDREs associated functions form the mathematical backbone of TURINGs work on morphogenesis. TURING was up to more than providing a mathematical description of initial stages of embryonic development. Rather he was eager to unveil the mathematical foundations of living, biological organization. An investigation of the archival material of unpublished letters and manuscripts indicates that
TY - JOUR. T1 - Homophilic Dscam Interactions Control Complex Dendrite Morphogenesis. AU - Hughes, Michael E.. AU - Bortnick, Rachel. AU - Tsubouchi, Asako. AU - Bäumer, Philipp. AU - Kondo, Masahiro. AU - Uemura, Tadashi. AU - Schmucker, Dietmar. PY - 2007/5/3. Y1 - 2007/5/3. N2 - Alternative splicing of the Drosophila gene Dscam results in up to 38,016 different receptor isoforms proposed to interact by isoform-specific homophilic binding. We report that Dscam controls cell-intrinsic aspects of dendrite guidance in all four classes of dendrite arborization (da) neurons. Loss of Dscam in single neurons causes a strong increase in self-crossing. Restriction of dendritic fields of neighboring class III neurons appeared intact in mutant neurons, suggesting that dendritic self-avoidance, but not heteroneuronal tiling, may depend on Dscam. Overexpression of the same Dscam isoforms in two da neurons with overlapping dendritic fields forced a spatial segregation of the two fields, supporting the ...
PLoS Comput Biol. 2013;9(11):e1003319. doi: 10.1371/journal.pcbi.1003319. Epub 2013 Nov 21. Research Support, N.I.H., Extramural
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A further aim of our study was to re-evaluate BMPs as prognostic factors for patients with osteosarcoma. Based on two previous studies,5, 6 BMPs have frequently been cited as prognostic markers over the past decade. These studies measured bone morphogenetic activity in human osteosarcoma specimens. Such activity was demonstrated as ectopic formation of new bone on implanted freeze dried fractions of human osteosarcomas into athymic nude mice. Ectopic bone formation was attributed to the production of BMPs in the tumour. The cohort of examined cases in these studies was small, consisting of 20 and 30 patients, respectively.5, 6 Bone morphogenetic activity was seen in approximately one third of patients in both studies. A correlation of bone morphogenetic activity with response to chemotherapy showed relative resistance to preoperative regimens consisting of adriamycin and methotrexate in patients with bone morphogenetic activity.5 In both studies, bone morphogenetic activity correlated with a ...
Model for different phases of mammary gland branching morphogenesis. Before puberty, the mammary epithelial is small and simply branched. In response to the rel
The Gene Expression and Morphogenesis (GEM-DMC2) aims at understanding how cells coordinate their behavior during development, homeostasis and evolution of animals to generate, sustain and modify organ form and function. GEM teams integrate analyses spanning from the genomic and subcellular to the organ and organism levels.. The Unit comprises the Department of Gene Regulation and Morphogenesis at the Centro Andaluz de Biología del Desarrollo (CABD Andalusian Centre for Developmental Biology (Seville); supported by CSIC, UPO and JA) plus Acaimo González-Reyes (Dept. Cell Biology and Biotechnology).. GEM teams have a very strong background in Developmental Genetics and Molecular Biology, and their projects make use of all major multicellular model organisms (C. elegans, Drosophila, Xenopus, Zebrafish, Medaka, and mouse). Some groups have promising projects using emerging animal models such as Mayfly, Astyanax (cavefish) and Killifish.. Research in the Unit is supported by state of the art ...
p130Cas adaptor protein regulates basic procedures such as for example cell cycle control, survival and migration. enough to re-establish branching morphogenesis and regular Erk1/2 MAPK activity. General, these outcomes indicate that high degrees of p130Cas appearance profoundly have an effect on mammary morphogenesis by changing epithelial architecture, success and unbalancing Erk1/2 MAPKs activation in response to development elements and human hormones. These results claim that alteration of morphogenetic pathways because of 701213-36-7 p130Cas over-expression might best mammary epithelium to tumorigenesis. Launch p130Cas, originally defined as an extremely phosphorylated proteins in cells changed by v-Src and v-Crk oncogenes, is normally a multifunctional adaptor proteins necessary Rabbit polyclonal to PID1 for embryonic advancement [1] and it is seen as a structural motifs that enable connections with a number of signaling substances. These multi-protein complexes feeling and integrate ...
p130Cas adaptor protein regulates basic procedures such as for example cell cycle control, survival and migration. enough to re-establish branching morphogenesis and regular Erk1/2 MAPK activity. General, these outcomes indicate that high degrees of p130Cas appearance profoundly have an effect on mammary morphogenesis by changing epithelial architecture, success and unbalancing Erk1/2 MAPKs activation in response to development elements and human hormones. These results claim that alteration of morphogenetic pathways because of 701213-36-7 p130Cas over-expression might best mammary epithelium to tumorigenesis. Launch p130Cas, originally defined as an extremely phosphorylated proteins in cells changed by v-Src and v-Crk oncogenes, is normally a multifunctional adaptor proteins necessary Rabbit polyclonal to PID1 for embryonic advancement [1] and it is seen as a structural motifs that enable connections with a number of signaling substances. These multi-protein complexes feeling and integrate ...
TY - JOUR. T1 - Factors Controlling Growth, Motility, and Morphogenesis of Normal and Malignant Epithelial Cells. AU - Birchmeier, Carmen. AU - Meyer, Dirk. AU - Riethmacher, Dieter. PY - 1995/1/1. Y1 - 1995/1/1. N2 - Factors that control epithelial growth, motility, and morphogenesis play important roles in malignancy and in normal development. Here we discuss the molecular nature and the function of two types of molecules that control the development and maintenance of epithelia: Components that regulate epithelial cell adhesion; and soluble factors and their receptors that regulate growth, motility, differentiation, and morphogenesis. In development, the establishment of epithelial cell characteristics and organization is crucially dependent on cell adhesion and the formation of functional adherens junctions. The integrity of adherens junctions is frequently disturbed late in tumor progression, and the resulting loss of epithelial characteristics correlates with the metastatic potential of ...
Current questions about the formation of organisms are the focus of Alexis Maizels group. Fundamental cell characteristics define the behavior of cell populations and organs and ultimately the so-called morphogenesis. Plant cells are connected with each other by their cell walls and are therefore immobile. As a result, they need to tightly coordinate their behavior. This high degree of complexity is the reason, why certain aspects of the control mechanisms of plant morphogenesis are still not completely unraveled. The research group Morphogenesis of Plants is funded with around 2.8 million euros and unites scientists from the fields of developmental biology, computer sciences and physics to obtain a holistic and quantitative understanding of tissue morphogenesis. The developmental plasticity of plants is one of Alexis Maizels research foci. Sabine Strahl investigates processes of glycosylation during which sugar molecules bind to proteins. These are one of the most important molecular ...
Objectives: The Rho subfamily of small GTPases, including RhoA, Rac1, and Cdc42, regulates diverse cellular functions, including polarity and migration. Our prior studies established an essential role for Cdc42 in vascular network assembly, demonstrating that the genetic inactivation of Cdc42 yields defective vascular morphogenesis due to impaired migration of endothelial precursor cells. We have further shown that protein kinase Ciota and glycogen synthase kinase-3 Beta are downstream effectors of Cdc42 involved in mediating vascular network assembly. The objective of this study was to elucidate the guanine nucleotide exchange factors (GEFs) that activate Cdc42; specifically, we investigated the role of Zizimin1 and its effects on Cdc42 and vasculogenesis.. Methods: We performed affinity pulldown assays using a nucleotide-free Cdc42 G15A mutant that specifically binds to Cdc42 GEFs. Mass spectrometric analysis identified Zizimin1 as a candidate Cdc42 GEF.. Results: During vasculogenesis in ...
Description (provided by applicant): The vertebrate skeleton is an intricate system of approximately 200 parts of distinct morphologies that are arranged to ensure fluid motion and to confer unique mechanical properties to the body. The long-term objectives of this research are to determine the molecular mechanisms that regulate morphogenesis of the individual skeletal elements and to elucidate how errors in these pathways result in skeletal defects. Defining the pathways that regulate skeletal morphogenesis will both aid the development of therapies to prevent birth defects and improve our ability to repair defective elements of the skeleton by enhancing methods in tissue engineering. The objectives of this proposal are to define the role of Frizzled (Fzd) signaling in the regulation of column formation during chondrocyte maturation and to determine the relationship between these processes and the morphology of cartilage elements. To achieve these objectives, the Specific Aims of this proposal ...
Tubulogenesis in epithelial organs often initiates with the acquisition of apicobasal polarity, giving rise to the formation of small lumens that expand and fuse to generate a single opened cavity. In this study, we present a micropattern-based device engineered to generate epithelial tubes through a process that recapitulates in vivo tubule morphogenesis. Interestingly, tubulogenesis in this device is dependent on microenvironmental cues such as cell confinement, extracellular matrix composition, and substrate stiffness, and our set-up specifically allows the control of these extracellular conditions. Additionally, proximal tubule cell lines growing on micropatterns express higher levels of drug transporters and are more sensitive to nephrotoxicity. These tubes display specific morphological defects that can be linked to nephrotoxicity, which would be helpful to predict potential toxicity when developing new compounds. This device, with the ability to recapitulate tube formation in vitro, has ...
Cell shape changes during cuticle formation. (A-A″) Comparison of bib and neur expression patterns. (A) GFP (green) and anti-β-gal immunostaining (magenta, shown in grey-scale in A′) in the joint region of a bib-GAL4/+; neur-lacZ/UAS-membrane-targeted-GFP pupa. The sample was fixed at a stage comparable to that shown in Fig. 3C,C′. neur expression is hardly detected in the distal portion of bib-expressing cells (bracket). (B-J) Membrane-targeted GFP is expressed under the control of bib-GAL4 (B,E,H), neur-GAL4 (C,F,I) or fng-GAL4 (D,G,J). Samples fixed at different stages of cuticle formation are shown. (B-D) The ball cuticle has started to grow (asterisk). (B) bib-expressing cells contact the dorsal part of the ball cuticle (arrow). (C) neur is expressed in both the dorsal subset of bib-expressing cells (arrow) and the cells located dorsal to them (arrowhead). (D) fng is expressed in cells projecting into the ridge. Arrowhead indicates the ventral edge of the fng expression domain. (E-G) ...
How neurons change their cytoskeleton to adopt their complex polarized morphology is still not understood. Growing evidence suggests that proteins that help build microtubule structures during cell division are also involved in building and remodeling the complex cytoskeletons of neurons. Kif20b (previously called MPP1 or Mphosph1) is the most divergent member of the Kinesin-6 family of
TY - CHAP. T1 - New insight into the regulation of floral morphogenesis. AU - Tsai, Wen Chieh. AU - Pan, Zhao Jun. AU - Su, Yong Yu. AU - Liu, Zhong Jian. PY - 2014. Y1 - 2014. N2 - The beauty and complexity of flowers have held the fascination of scientists for centuries, from Linnaeus, to Goethe, to Darwin, through to the present. During the past decade, enormous progress has been made in understanding the molecular regulation of flower morphogenesis. It seems likely that there are both highly conserved aspects to flower development in addition to significant differences in developmental patterning that can contribute to the unique morphologies of different species. Furthermore, floral development is attractive in that several key genes regulating fundamental processes have been identified. Crucial functional studies of floral organ identity genes in diverse taxa are allowing the real insight into the conservation of gene function, while findings on the genetic control of organ elaboration ...
I. Slavkov, D. Carrillo-Zapata, N. Carranza, X. Diego, F. Jansson, J. Kaandorp, S. Hauert and J. Sharpe. Morphogenesis allows millions of cells to self-organize into intricate structures with a wide variety of functional shapes during embryonic development. This process emerges from local interactions of cells under the control of gene circuits that are identical in every cell, robust to intrinsic noise, and adaptable to changing environments. Constructing human technology with these properties presents an important opportunity in swarm robotic applications ranging from construction to exploration. Morphogenesis in nature may use two different approaches: hierarchical, top-down control or spontaneously self-organizing dynamics such as reaction-diffusion Turing patterns. Here, we provide a demonstration of purely self-organizing behaviors to create emergent morphologies in large swarms of real robots. The robots achieve this collective organization without any self-localization and instead rely ...
Networks of epithelial tubes, such as the vertebrate lung, kidney and vascular system, enable transport of gases and nutrients to all tissues in the body. These tubes are built up by a single layer of polarized epithelial cells, with the apical membrane facing the lumen. For optimal organ function it is critical that each tube in the network attains correct size and shape, as constricted or dilated tubes will affect the flow rate and impede organ function. When tubes form during organ development, they often have a narrow lumen that must expand to attain the typical mature length and diameter. Both apical (luminal) membrane growth and rearrangements of the subapical cytoskeleton are central to tube growth, but the mechanisms that coordinate these events across the tubular epithelium to ensure uniform tubes with functional dimensions have remained unknown. We have used the respiratory organ (trachea) of the fruit fly Drosophila melanogaster as a model system to gain insights into the molecular ...
Based on natural principles, we developed a novel toothpaste, made up of morphogenetically active amorphous calcium polyphosphate (polyP) microparticles which are enriched with retinyl acetate (a-polyP/RA-MP). not display any growth inhibitory activity against gene expression, which together with the gene is usually a marker for differentiation of the mesenchymal stem cells (MSC) into odontoblasts [33]. These beneficial properties of dentoReseal? should qualify the dentifrice as a solution for treating Rabbit Polyclonal to XRCC2 dentin and teeth enamel hypersensitivity and caries, so that as a morphogenetic agent helping the reconstitution from the periodontium, by inducing collagen gene appearance in the tissues specifically. 2. Methods and Materials 2.1. Components Sodium polyphosphate (Na-polyP of the average string of 40 phosphate products) was extracted from Chemische Fabrik Budenheim (Budenheim, Germany); retinyl acetate (syn. retinol acetate, supplement A acetate) dissolved to 50% ...
Dive into the research topics of The serine/threonine kinase, Krct, affects endbud morphogenesis during murine mammary gland development. Together they form a unique fingerprint. ...
Research groupsGene regulation and morphogenesis Cell integration of diverse genetic inputs during the morphogenesis of complex organs Dr ..
TY - JOUR. T1 - Self-organization of engineered epithelial tubules by differential cellular motility. AU - Mori, Hidetoshi. AU - Gjorevski, Nikolce. AU - Inman, Jamie L.. AU - Bissell, Mina J.. AU - Nelson, Celeste M.. PY - 2009/9/1. Y1 - 2009/9/1. N2 - Patterning of developing tissues arises from a number of mechanisms, including cell shape change, cell proliferation, and cell sorting from differential cohesion or tension. Here,wereveal that differences in cell motility can also lead to cell sorting within tissues. Using mosaic engineered mammary epithelial tubules, we found that cells sorted depending on their expression level of the membrane-anchored collagenase matrix metalloproteinase (MMP)-14. These rearrangements were independent of the catalytic activity of MMP14 but absolutely required the hemopexin domain. We describe a signaling cascade downstream of MMP14 through Rho kinase that allows cells to sort within the model tissues. Cell speed and persistence time were enhanced by MMP14 ...
Tomato leaves undergo extended morphogenesis, resulting in elaborate and variable leaf forms (Burko and Ori, 2013). Among tomato leaf phenotypes, the clau mutant is extreme in its leaf elaboration, exemplifying the potential of further extending the tomato leaf morphogenetic window and its possible results. Overexpressing CLAU curbs leaf morphogenesis, demonstrating that CLAU regulates the length of the leaf morphogenetic window.. How does CLAU regulate morphogenetic competence in the leaf? We recently reported that CLAU is involved in delimiting the expression of GOB, a central regulator of leaf morphogenesis (Bar et al., 2015; Ben-Gera et al., 2012; Berger et al., 2009; Brand et al., 2007; Busch et al., 2011; Rossmann et al., 2015). Here, GOB is shown to be downregulated by CLAU overexpression. In addition, clau has abnormally high expression of the KNOXI gene LeT6/Tkn2, which plays important role in promoting leaf morphogenesis (Avivi et al., 2000; Jasinski et al., 2007). Here, we show that a ...
dendrite, dendritic spine, neuron projection, neuron spine, postsynaptic density, GKAP/Homer scaffold activity, ionotropic glutamate receptor binding, receptor signaling complex adaptor activity, brain morphogenesis, dendritic spine morphogenesis
GATA-4 is a key regulator of a poorly understood cardiac morphogenetic program. We used genomic regions of the GATA-4 gene to target GFP expression to the devel
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All tissues of the body originate from a ball of pluripotent cells, located in the inner of cell mass of the late blastocyst. We found previously unknown morphogenic events polarization, apical constriction and lumenogenesis that reorganize the embryonic lineage during implantation. These morphogenic changes can be induced and studied in ES cells grown in 3D culture, when proper polarization cues are provided. Our findings are contrary to the long term believe that programed cell death transforms the epiblast from a simple ball of cells into cup shaped epithelium.. Funding: Wellcome trust. ...
Purpose: Previously, we identified the transcription factors Sox11 and Sox4 as candidate regulators of rod photoreceptor regeneration in zebrafish. Sox11 and Sox4 are members of the SoxC family of HMG-box containing transcriptional regulators. SoxC factors are known to regulate neurogenesis in the brain and spinal cord, and are also required for the proper development of several organ systems. However, their function during ocular development was not well understood. In this study, we investigated the role of Sox11 and Sox4 during ocular morphogenesis in zebrafish.. Methods: We used a combination of morpholino-mediated gene knockdown, mRNA over-expression, reporter gene expression, and pharmacologic inhibition of Hh signaling, to analyze the role of SoxC genes during ocular development. Gene expression in zebrafish embryos was analyzed by quantitative RT-PCR, by whole mount in situ hybridization, and by fluorescent in situ hybridization on tissue sections. Retinal cell type differentiation was ...