TY - JOUR. T1 - Molecular Probe Data Base (MPDB). AU - Campi, Maria Giuseppina. AU - Romano, Paolo. AU - Milanesi, Luciano. AU - Marra, Domenico. AU - Manniello, Maria Assunta. AU - Lannotta, Beatrice. AU - Rondanina, Gabriella. AU - Grasso, Elena. AU - Ruzzon, Tiziana. AU - Santi, Leonardo. PY - 1998/1/1. Y1 - 1998/1/1. N2 - In this paper, the current status of the Molecular Probe Data Base (http://www.biotech.ist.unige.it/interlab/mpdb.html) is briefly presented together with a short analysis of its activity during 1997. This has been performed by statistically evaluating the logs of the Internet servers that are used for its distribution with reference to the geographical origin of the requests, the words that were utilized to carry out of the searches and the oligonucleotides that were retrieved. Planned enhancements of this database are also described. They include a revision of its data structure and, even more relevant, of its data management procedures.. AB - In this paper, the current ...
Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Invitrogen™ Molecular Probes™ |i|Taq|/i| DNA Polymerase, Native 3 x 500 units Invitrogen™ Molecular Probes™ |i|Taq|/i| DNA...
Diacylglycerol lipase (DAGL)-α and -β are enzymes responsible for the biosynthesis of the endocannabinoid 2-arachidonoylglycerol. Selective and reversible inhibitors are required to study the function of DAGLs in neuronal cells in an acute and temporal fashion. The work described in this thesis has led to the identification of an activity-based probe (ABP) for DAGL-α. This ABP was instrumental for the identification of a new class of highly selective DAGL inhibitors. These novel inhibitors can be used to investigate the role of the diacylglycerol lipases in metabolic syndrome and neurodegenerative diseases such a Parkinsons disease, Alzheimers disease and Multiple Sclerosis ...
BACKGROUND: In approximately 10% of all gastric cancer (GC) cases, a heritable cause is suspected. A subset of these cases have a causative germline CDH1 mutation; however, in most cases the cause remains unknown. Our objective was to assess to what extent these remaining cases may be explained by germline mutations in the novel candidate GC predisposing genes CTNNA1, MAP3K6 or MYD88. METHODS: We sequenced a large cohort of unexplained young and/or familial patients with GC (n=286) without a CDH1germline mutation for germline variants affecting CTNNA1, MAP3K6 and MYD88 using a targeted next-generation sequencing approach based on single-molecule molecular inversion probes ...
... ,Alexa Fluor 350/488/594 Filter Set (Semrock Brightline) *mounted* *Zeiss Axio AF,biological,biology supply,biology supplies,biology product
Molecular Probes APEX Alexa Fluor 647 Antibody Labeling Kit 1 kit Life Sciences:Protein Biology:Crosslinking, Labeling and Protein Modification:Protein Labeling Reagents:Fluorescent
A variety of molecular probes were synthesized and characterized with the goal of gaining structural, conformational, and mechanistic information on polymerases. The leaving group effects of β,γ-CXY deoxynucleotide probes was investigated in order to determine the rate-determining step in the nucleotidyl mechanism of pol β. Due to inconsistencies in literature the pKa₍₂₋₄₎ values for nine bisphosphonic acids used to comprise the β,γ−CXY toolkit were determined under identical conditions. Applying these acidity constants to the analysis of kinetic results obtained with a series of dGTP-β,γ−CXY analogues clarified the relationship between the rate of single-gap nucleotide insertion and BP pKa₄ values. Through construct of Brønsted LFER plot results seem to indicate that chemistry and not conformation is the rate-limiting step in the nucleotidyl transfer mechanism. ❧ Modification on the Pᵧ of α,β-CH₂ non-hydrolyzable dATP analogs were synthesized to probe the ...
A new fluorescence-based molecular probe can detect multiple sclerosis (MS) at its earliest stage - even before the onset of physical signs.
Molecular Probes Cell Imaging Guide with Protocols brbrThis mobile app is designed to help you find fluorescent dyes reagents and protocols for cell biology related fluorescence microscopy applications brbrMolecular Probes mobile app
Researchers at the Stanford University School of Medicine have developed a molecular probe that sets aglow tumor cells within living animals. Their goal is to use the probe to improve the diagnosis and treatment of cancer and other diseases.
... for Accelerated Probe Development is a member of the MLPCN, initiated and supported by the NIH. Centers mission is to develop small molecule probes as molecular targets with exceptional potency and selectivity for exploring the functions of genes and pathways in health and disease at the molecular and cellular and in vivo levels.
Chemical engineers at UC Santa Barbara expect that their new process to create molecular probes may eventually result in the development of new drugs to treat cancer and other illnesses.
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Our research involves the chemical biology of protein post-translational modifcations (PTMs) in the context of signaling, epigenetics, and cancer. We develop and apply chemical approaches including protein semisynthesis and small molecule probes to the study of protein phosphorylation, acetylation, ubiquitination, and other PTMs in enzymes and cellular networks. We are currently investigating the functions, regulation, and mechanisms of PTEN lipid phosphatase, Akt protein kinase, NEDD4 ubiquitin ligases, LSD1 histone demethylase, HDAC1 deacetylase, the CoREST complex, and p300/CBP acetyltransferase. We strive to translate our findings in signaling and epigenetics to identify novel therapeutic opportunities for the treatment of cancer and other diseases ...
Principal Investigator:IDE Hiroshi, Project Period (FY):1997 - 1999, Research Category:Grant-in-Aid for Scientific Research (B), Section:展開研究, Research Field:環境影響評価(含放射線生物学)
CHANGZHOU, China - On a dusty lane in east China, a small factory sitting amid strawberry and vegetable fields processes chemicals from pig guts into heparin, a commonly used blood thinner linked to 62 deaths and hundreds of allergic reactions in the U.S. and Germany ...
1. Make sure you or your loved one is actively participating in therapy. If not, the benefits of therapy will be very minimal. Passive therapy should be implemented only for a short time and a short duration.
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We now coverslip with Molecular Probes Prolong Gold antifade reagent, with or without DAPI. We have found some antifade mounting medias cause fading if left overnight, but if the slides are examined right after staining, fading has not occured. So be careful about what antifade mounting medias you use. Also, some fluorophores do not withstand mounting medias and will fade right away. I believe Cy2 is one, and also Alexa 630 - Jackson and Molecular Probes provide this information. We have found RRX (rhodamine red extra) from Jackson Immunorearch, is more reisistant to staining than TRITC, both are derivatives from fluorescein but have different chemical structures. We dont bother to refrigerate but do keep the sections in the dark and try to view them the next day after staining,coverslipping. Selection of fluorophore is important, and Alexa dyes are more resistant to fading and also much brighter than fluorescein related fluorophores (FITC, Rhodamine, Texas Red). It is important to learn about ...
Accurate localization of diseases in humans, especially at advanced stages, is widely accomplished by modern imaging methods such as magnetic resonance imaging...
Principal Investigator:KOBAYASHI Motomasa, Project Period (FY):2003 - 2004, Research Category:Grant-in-Aid for Scientific Research (B), Section:一般, Research Field:Living organism molecular science
Molecular Probes offers a Yeast Viability Kit (Product number is 7009) ------------------------------------------------ Mahesh K. Bhalgat, Ph.D. Staff Scientist/Flow Cytometry Product Manager Molecular Probes, Inc. OREGON ------------------------------------------------ John wrote: , Hi All, , , A coleague of mine is interested in doing yeast viability assays on , our , Coulter XL, , Does anybody have a good system for this, we have tried PI but it , appears , that it does not cross the cell wall to easily. , , Many Thanks , , John ...
FluoroNanogold is a unique probe containing both Nanogold® and fluorescein. You can use it for truly correlative labeling on the cellular and macromolecular level, or for checking your labeling by fluorescence before EM processing. Catalog information about FluoroNanogold: www.nanoprobes.com/FluoroN.html Because FluoroNanogold combines two labels which are frequently used in different concentrations under different conditions, some optimization may be required to obtain the best performance in your experiments. For tips and tricks on FluoroNanogold labeling, and how to obtain the cleanest signal, visit our technical help page: Technical help for FluoroNanogold: www.nanoprobes.com/TechFN.html Look out for combined fluorescent and gold probes with other fluorophores: when these are available, we will announce them on our web site (www.nanoprobes.com). Original papers: Powell, R. D.; Halsey, C. M. R.; Spector, D. L.; Kaurin, S. L.; McCann, J.;, and Hainfeld, J. F. A covalent fluorescent-gold ...
Angela Koehler is an institute member at the Broad Institute of MIT and Harvard and a member of the steering committee for the Broads Cancer Program. Her research group aims to discover and develop functional small-molecule probes of transcriptional regulators, including chromatin modifying enzymes and oncogenic transcription factors. Validated probes may be used to advance
Rutgers University June 26, 2017 Todd B. Bates New DNA-based LASSO molecule probe can bind target genome regions for functional cloning and analysis. Photo: Jennifer E. Fairman/JHU Discovering the function of a gene requires cloning a DNA sequence and expressing it. Until now, this was performed on a one-gene-at-a-time basis, causing a bottleneck. Scientists at…
Clearly the researchers didnt start out trying to disprove the role of DDR1/2 in squamous cell lung cancer, but their efforts will save others from pursuing the same course. The publication introduces some attractive chemical probes for interrogating the biology of these receptors; hopefully one of these molecules will be added to the Chemical Probes Portal as an alternative to the less-selective probes that have been used in previous studies. Who knows, perhaps someone will find another indication for which DDR1/2 inhibitors are just the ticket ...
koteswararao2kanugula at gmail.com wrote: , Dear sir, , This is koteswara rao , I am facing the problem of , coupling reaction as well as identification of the hapten + protien , coupling. Both Molecular Probes (www.probes.com) and Pierce (www.piercenet.com) discuss cross-linkers in their respective catalogues, which are available online ...
Also the Alexa kits from Molecular Probes. Typically, you need 100 ug of protein, although there is a microscale labeling kit that works with as little as 20 ug, if I remember correctly.. ...
Southern blot definition is - a blot consisting of a nitrocellulose or nylon sheet containing spots of DNA for identification by a suitable molecular probe.
The authors used time-driven activity-based costing method to calculate the costs of treating individual patients with specific medical conditions over their full cycle of care. We discussed how TDABC provides a critical, systematic and data-driven approach to estimate costs accurately and dynamically, as well as its potential to enable structural and rational cost reduction to bring about a sustainable healthcare system.
Activity-based costing (ABC) is a costing model that identifies activities in an organization and assigns the cost of each activity resource to all products an…
Free Essay: Activity-Based Costing (ABC) in Service Industries H & R Block H & R Block A Study of TDABC Costing Robert Kaplan defines activity based...
Fluorescent Molecular Rotors are sensors of microenvironmental restriction that become fluorescent only if their rotation is constrained. It has been suggested that the change of fluorescence intensity is caused by the restriction of intramolecular rotational relaxation about the donor-acceptor bond of the fluorophores.
Examples of molecular constraint include increased dye (aggregation)1, binding to antibodies2, or being trapped in the polymerization of actin3. There are also studies of membrane fluidity in endothelial cells under fluid shear stress4 where fluorescent molecular rotors are used.

1. Bhattacharyya, A. et al., Indian J. Biochem. Biophys., 32, 442-446 (1995).
2. Iwaki, T. et al., Biochem., 32, 7589-7592 (1993).
3. Sawada, S. et al., Anal. Biochem., 204, 110-117 (1992).
4. Farkas, D.L. and Leif R.C. (ed.), Optical diagnostics of living cells III, Proc SPIE 3291, 101-112 (2000
Translocations affecting chromosome subband 6p25.3 containing the IRF4 gene have been recently described as characteristic alterations in a molecularly distinct subset of germinal center B-cell-derived lymphomas. Secondary changes have yet only been described in few of these lymphomas. Here, we performed array-comparative genomic hybridization and molecular inversion probe microarray analyses on DNA from 12 formalin-fixed paraffin-embedded and two fresh-frozen IRF4 translocation-positive lymphomas, which together with the previously published data on nine cases allowed the extension of copy number analyses to a total of 23 of these lymphomas. All except one case carried chromosomal imbalances, most frequently gains in Xq28, 11q22.3-qter, and 7q32.1-qter and losses in 6q13-16.1, 15q14-22.31, and 17p. No recurrent copy-neutral losses of heterozygosity were observed. TP53 point mutations were detected in three of six cases with loss of 17p. Overall this study unravels a recurrent pattern of ...
Fluorescent molecular imaging helped surgeons visually identify lung adenocarcinomas during pulmonary resection, according to study results.
Molecular Probes fluorescence reagents are among the most peer-referenced in all of life science research. Trusted product bands including Alexa Fluor, SYTOX and Click-iT empower scientists working in cell biology, genetic analysis, protein biology, and cell engineering to make the discoveries that advance our understanding today and catalyze the research goals of tomorrow.
Molecules with ionizable protons, with different proton transfer efficiencies in the excited and ground states, show excited state prototropism (ESPT). In suitable proton donating/accepting environments, ESPT of a molecule can result in the observation of emissions from different prototropic species, each ch 2019 Perspective article collection
Surface properties of silica gel and silica gel-supported ion-exchanged copper in transformations of various molecular probes: an infrared study ...
During the last decade, the development of nuclear spin polarization enhanced (hyperpolarized) molecular probes has opened up new opportunities for studying the inner workings of living cells in real time. The hyperpolarized probes are produced ex situ, introduced into biological systems and detected with high sensitivity and contrast against background signals using high resolution NMR spectroscopy. A variety of natural, derivatized and designed hyperpolarized probes has emerged for diverse biological studies including assays of intracellular reaction progression, pathway kinetics, probe uptake and export, pH, redox state, reactive oxygen species, ion concentrations, drug efficacy or oncogenic signaling. These probes are readily used directly under natural conditions in biofluids and are often directly developed and optimized for cellular assays, thus leaving little doubt about their specificity and utility under biologically relevant conditions. Hyperpolarized molecular probes for biological NMR
My research group is engaged at the interface of chemistry and biology. As medicinal chemists, we design and synthesize small molecules (about 25 to 75 atoms) that modulate disease pathology at the level of the enzyme, cell, and whole animal, with current projects in cancer, infectious disease, and neurodegeneration. Using such small molecule probes, we are exploring the mechanisms underlying the action of artemisinin and related antimalarial drugs. Finally, we are inventing and employing new platform technologies in an effort to expand the realm of druggable target space and drug-like chemical space. These technologies include fragment-based approaches to target protein-protein interfaces and allosteric modulation of enzymes, the use of novel unnatural amino acids to discover cell-permeable peptides, and new targeted prodrug approaches in cancer and infectious disease.. This project is an evaluation of ferrous-iron targeted drug delivery that exploits the high ferrous iron concentrations in ...
Proteases are one of the largest and best-characterized families of enzymes in the human proteome. Unfortunately, the understanding of protease function in the context of complex proteolytic cascades remains in its infancy. One major reason for this gap in understanding is the lack of technologies t …
여기서는 활동 기반 프로브의 제조 및 사용을 설명 (ARN14686, 운데 10 ynyl- N - [(3- S) -2- oxoazetidin -3- 일] 카르 바 메이트)의 검출 및 정량화를 허용 염증성 효소 ...
For decades researchers have been using the famous Alexa Fluor dyes developed by Molecular Probes for everything from flow cytometry to oligonucleotide labeling. The original Molecular Probes patents have likely limited the number of companies offering less expensive alternatives to the Alexa Fluor dyes. Innovative dyes as DyLight and IRDyes have been developed and are used for specific applications, but direct alternatives for the standard Alexa Fluor exitation and emission spectra dyes have been difficult/impossible to find ..until now. ...
Reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Can replace Alexa Fluor 647, DyLight 649 ...
The SYPRO dyes, which were developed by Molecular Probes, bind to the lipid coating around proteins in SDS gels and not to the protein itself. I would not expect any interaction with 35-S. If necessary, the dyes can be extracted from the proteins with organic solvents such as alcohol and, following transfer, do not interfere with immunoblotting. Dick Haugland, President Molecular Probes, Inc. dick at probes.com ...
The MALDI Tissuetyper is a system that records spatially resolved mass spectra directly from tissue. This allows the direct measurement of proteins, lipids and other molecular classes without the need for antibodies or molecular probes. This results in highly multiplexed datasets in which hundreds or thousands of compounds are measured simultaneously. , The MALDI Tissuetyper is a system that records spatially resolved mass spectra directly from tissue. This allows the direct measurement of proteins, lipids and other molecular classes without the need for antibodies or molecular probes. This results in highly multiplexed datasets in which hundreds or thousands of compounds are measured simultaneously.
H2S - along with nitric oxide, carbon monoxide and others in this emerging class of gaseous signaling molecules - assists the bodys large proteins. Large proteins do much of the functional work in the body, such as digesting the food we eat and harnessing the energy in the oxygen we breathe. Their size, however, forces them to move slowly inside the cell. In contrast, H2S and other small gaseous molecules diffuse quickly and easily across cellular membranes, enabling them to travel much faster and rapidly deliver information that mediates critical functions, such as blood pressure regulation, Lippert said.. For their experiments, Lippert and Lin placed living endothelial cells cultured from the internal lining of a blood vessel into a petri dish under a microscope. Lippert and Lin then added a chemical solution containing an azide-functionalized organic molecule that theyd synthesized to act as a molecular probe. They gave the cells time to absorb the probe, then added a protein solution known ...
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Results of molecular beacon end-point assays using the E8/E9 and E9/E10 probes on a representative genomic DNA panel. There is no difficulty in distinguishing a
Protean agonists are of great pharmacological interest as their behavior may change in magnitude and direction depending on the constitutive activity of a receptor. Yet, this intriguing phenomenon has been poorly described and understood, due to the lack of stable experimental systems and design strategies. Here, we overcome both limitations: First, we demonstrate that modulation of the ionic strength in a defined experimental set-up allows for analysis of GPCR activation in the absence and presence of a specific amount of spontaneous receptor activity using the muscarinic M2 acetylcholine receptor as a model. Second, we employ this assay system to show that a dualsteric design principle, i.e. molecular probes, carrying two pharmacophores to simultaneously adopt orthosteric and allosteric topography within a G protein-coupled receptor, may represent a novel approach to achieve protean agonism. We pinpoint three molecular requirements within dualsteric compounds that elicit protean agonism at the ...
A central pathogen virulence mechanism is their ability to secrete pathogen proteins, called effectors, that modulate their host during infection. We are using effectors as molecular probes to better understand how bacteria cause disease. We are also investigating the role of post-translational modifications during plant immune signaling. ...
This note discusses the expected contributions of the activity-based research approach to the general development of transport planning. Against a brief review of the development of the approach and its central tenets it discusses four areas in turn: data collection, models of household interactions, choice models incorporating the whole daily activity chain and finally explicit micro-simulation models of activity scheduling. The final section highlights the expected contributions and the possible difficulties of their acceptance into the planning process.
A probe driving method and a probe apparatus for bringing a probe into contact with the surface of a sample in a safe and efficient manner by monitoring the probe height. Information about the height of the probe from the sample surface is obtained by detecting a probe shadow (54) appearing immediately before the probe contacts the sample, or based on a change in relative positions of a probe image and a sample image that are formed as an ion beam is irradiated diagonally.
The GA4GH (Global Alliance for Genomics and & Health) Beacon Projectis a project to encourage international sites to share genetic data in the simplest of all technical contexts. The service is designed merely to accept a query of the form "Do you have any genomes with an A at position 100,735 on chromosome 3" (or similar data) and responds with one of "Yes" or "No.". The Beacon Network lists all the known beacons.. For programmatic access to the COSMIC beacon, the minimal URL for the request is ...
Production of near-field microscopy gets scaled up under the direction of experts at The Molecular Foundry at Lawrence Berkeley Laboratory
This site provides an animated simulation to learn about the use of a probe to locate genes in specific organisms. A gene sequence is presented and step-by-step instructions are used to explain the uniqueness of DNA and how probes are designed. ...
Chi- squared is also know as the goodness of fit, this means that it tests the shape and proportion of the data in relation to the null. the goodness of fit if tested by comparing the observed frequencies with the distribution set in the null hypothesis. chi squared in my eyes is the most understandable of all the statistical tests as there is no…
Tektronix Current Probes provide high sensitivity for accurate power and load analysis. Find the right Current Probe for your measurement needs.
The purpose of this study is to answer two main questions: Is switching behaviour in Activity-based working (ABW) environments related to satisfaction with ABW environments? Which factors may explain switching behaviour?
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OBJECTIVES:. I. Validation of the 15-gene prognostic and predictive messenger (m) ribonucleic acid (RNA) signature on Lung Adjuvant Cisplatin Evaluation (LACE)-Bio formalin-fixed, paraffin-embedded tissue (FFPE) tumor samples.. II. Exploratory evaluation of the prognostic and predictive values of known potential oncogenic mutations using LACE-Bio FFPE tumor samples.. III. Exploratory evaluation of the prognostic and predictive values of gene copy variations using LACE-Bio FFPE tumor samples.. IV. Exploratory identification and evaluation of prognostic and predictive value of novel genomic aberrations discovered by Next Generation Sequencing on LACE-Bio tumor samples.. OUTLINE:. Archived RNA and DNA samples are analyzed for gene expression, mutations, and variations by reverse transcriptase (RT)-polymerase chain reaction (qPCR), mass spectrometry (MassARRAY), molecular inversion probe assay, and microarray assays. Results are then compared with patients clinical outcomes. ...
The accurate identification of taxa from mixed assemblages using genetic analysis remains an important field of molecular biology research. The common principle behind the development of numerous documented genetic detection technologies is to exploit specific nucleotide sequences inherent to each taxon. This body of work focuses on practical applications of real-time nucleic acid sequence-based amplification (RT-NASBA) in marine science, and is presented in four case studies. Each study represents novel work in the genetic identification of respective taxa of interest using RT-NASBA. Two case studies documented the development of an assay targeting mitochondrial 16S rRNA to discern legally salable grouper species in the U.S. from fraudulently mislabeled surrogate fish. This technology was first validated using lab-based, benchtop instrumentation, and was then adapted into a complete field detection system. The third study documented an internally controlled RT-NASBA (IC-NASBA) assay for the
Malaria is a devastating disease that impacts approximately 215 mil sufferers annually among whom around 650 0 pass away (Who all 2011 The pass on of the condition can normally end up being controlled by way of a mix of vector control and chemotherapy. bioactivities natural basic products are actually valuable lead Cangrelor (AR-C69931) manufacture buildings for drug breakthrough (Mayer et al. 2010 Their additional development into medications is however frequently hampered by way of a lack of knowledge of their mode of action. As a result several strategies for the recognition of the direct focuses on of bioactive natural products have been developed in the past few years (Lomenick et al. 2011 Rix and Superti-Furga 2009 Among them proteome labeling strategies such as activity-based protein profiling (ABPP) have evolved into reliable tools for the recognition of protein focuses on of potentially bioreactive natural small molecules (B?ttcher et al. 2010 Cravatt et al. 2008 Deu et al. 2012 Heal et ...
Pseudomonas syringae pv. tomato DC3000 (PtoDC3000) is an extracellular model plant pathogen, yet its potential to produce secreted effectors that manipulate the apoplast has been under investigated. Here we identified 131 candidate small, secreted, non-annotated proteins from the PtoDC3000 genome, most of which are common to Pseudomonas species and potentially expressed during apoplastic colonization. We produced 43 of these proteins through a custom-made gateway-compatible expression system for extracellular bacterial proteins, and screened them for their ability to inhibit the secreted immune protease C14 of tomato using competitive activity-based protein profiling. This screen revealed C14-inhibiting protein-1 (Cip1), which contains motifs of the chagasin-like protease inhibitors. Cip1 mutants are less virulent on tomato, demonstrating the importance of this effector in apoplastic immunity. Cip1 also inhibits immune protease Pip1, which is known to suppress PtoDC3000 infection, but has a lower
We offer Amine-Reactive Fluorophores; Bioconjugates; Biotin/Desthiobiotin; Buffers, Detergents; Caged Probes; Cell Analysis Kits; Cell stains; Click Chemistry; Enzyme Substrates; Fluorescent Mitochondrial Probes; Fluorescent Probes; Fluorescent/Non-Fluorescent Ion and pH Indicators; Fluorescent/Non-Fluorescent Lipids, Phospholipid Probes; Gel & Membrane Stains; Kits and Assays; Monomers; Natural Products and Their Fluorescent/None-Fluorescent Conjugates; Neurotransmitter receptors Probes; Nucleotides/Nucleosides; Protein Kinase Inhibitors (PKI); Quencher/Non-Fluorescent Probes; Reactive Probes and Related Products; Sensors; Steroids and Related Products
We offer Amine-Reactive Fluorophores; Bioconjugates; Biotin/Desthiobiotin; Buffers, Detergents; Caged Probes; Cell Analysis Kits; Cell stains; Click Chemistry; Enzyme Substrates; Fluorescent Mitochondrial Probes; Fluorescent Probes; Fluorescent/Non-Fluorescent Ion and pH Indicators; Fluorescent/Non-Fluorescent Lipids, Phospholipid Probes; Gel & Membrane Stains; Kits and Assays; Monomers; Natural Products and Their Fluorescent/None-Fluorescent Conjugates; Neurotransmitter receptors Probes; Nucleotides/Nucleosides; Protein Kinase Inhibitors (PKI); Quencher/Non-Fluorescent Probes; Reactive Probes and Related Products; Sensors; Steroids and Related Products
Radioisotopes. Copper (Cu) is a key nutrient to human cells. Human Cu transporter 1 (CTR1), a 190-amino acid protein of 28 kDa with three transmembrane domains, is the primary protein responsible for import of Cu in mammal. Interestingly, Cu metabolism is also essential for many cancers, and it has been found that CTR1 is over-expressed in a variety of cancer cells including non-small cell lung cancer (NSCLC) and liver cancer. Over-expression of CTR1 has also been found to be associated with better cisplatin based chemotherapy response and favorable treatment outcome in lung cancer patients. Traditionally, 64Cu2+ has been widely used in labeling of peptides, proteins, antibodies, nanoparticles and other biologically relevant small molecules through a variety of bifunctional chelators such as DOTA and TETA. Recently, with the better understanding the role of CTR1 in tumor biology, 64CuCl2 has been reported as a novel and promising PET probe for imaging of Wilsons disease, prostate cancer, and ...
Similarity of skin cancer with many benign skin pathologies requires reliable methods to detect and differentiate the different types of these lesions. Previous studies have explored the use of disparate optical techniques to identify and estimate the invasive nature of melanoma and basal cell carcinoma with varying outcomes. Here, we used a concerted approach that provides complementary information for rapid screening and characterization of tumors, focusing on squamous cell carcinoma (SCC) of the skin. Assessment of in vivo autofluorescence lifetime (FLT) imaging of endogenous fluorophores that are excitable at longer wavelengths (480 nm) than conventional NADH and FAD revealed a decrease in the short FLT component for SCC compared to normal skin, with mean values of 0.57±0.026 ns and 0.61±0.021 ns, respectively (p=0.004). Subsequent systemic administration of a near-infrared fluorescent molecular probe in SCC bearing mice, followed by the implementation of image processing methods on data ...
Tissue immunostaining is critically essential in clinical applications, and antibodies have already been used as the molecular probes extensively. tumor areas occurred. Weighed against regular antibody staining, our EpCAM aptamer SYL3C process is very simple to put into action using a shorter response time. Moreover, SYL3C may bind with either frozen or paraffin-embedded tissues areas specifically. Because the histopathology of iced tissue is nearer to that of clean tissues and since iced areas can be created quicker than paraffin-embedded areas, SYL3C immunostaining of iced areas is an instant process that is simple to put into action. Cancer histopathology happens to be the preferred way for discovering microscopic anatomical adjustments in tissue areas, producing the breakthrough of cancers biomarkers crucial for early treatment and medical diagnosis, and antibodies have already been used as the molecular probes extensively.1 Epithelial cell adhesion molecule (EpCAM) is a transmembrane ...
This product is provided under an agreement between Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corporation), and Exbio Praha, a.s., and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by Molecular Probes, Inc. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity), including use in flow cytometry that does not utilize a bead based array, but excluding use in combination with microarrays or High Content Screening. The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for ...
This product is provided under an agreement between Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corporation), and Exbio Praha, a.s., and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by Molecular Probes, Inc. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity), including use in flow cytometry that does not utilize a bead based array, but excluding use in combination with microarrays or High Content Screening. The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for ...
Pyrimidine- and purine-rich strands of Mus musculus satellite DNA prepared by alkaline CsCl-gradient centrifugation can self-renature to a variable extent to give partial duplexes with high thermal stability. These duplexes were purified by treatment with nuclease S1 followed by hydroxylapatite chromatography, and have been shown by pyrimidine-tract analysis to be very similar in sequence to total reassociated satellite DNA. It is believed that the self-renaturing fractions result from variable contamination of each strand with fragments of the other, rather than from molecular inversions. The predominantly single-stranded properties of these fractions may be partly due to the ability of mouse satellite DNA strands to reassociate in non-stoicheiometric proportions.. ...
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A book for anyone who wants to learn programming to explore and create, with exercises and projects to help the reader learn by doing. This book introduces programming to readers with a background in the arts and humanities; there are no prerequisites, and no knowledge of computation is assumed. In it, Nick Montfort reveals programming to be not merely a technical exercise within given constraints but a tool for sketching, brainstorming, and inquiring about important topics. He emphasizes programmings exploratory potential-its facility to create new kinds of artworks and to probe data for new ideas. The book is designed to be read alongside the computer, allowing readers to program while making their way through the chapters. It offers practical exercises in writing and modifying code, beginning on a small scale and increasing in substance. In some cases, a specification is given for a program, but the core activities are a series of
... are hybridization probes that are used in a real time PCR assay for quantification of the target DNA. Molecular Beacons are single stranded and bind to the target when the target sequence is complementary
Researchers at Stanford University have demonstrated for the first time the use of a dual optical and PET/CT activity-based probe, which images vascular inflammation, to detect atherosclerotic plaques. The study is published in the October issue of "The Journal of Nuclear Medicine."
A phase-shifted coil comprises nested concentric spiral portions, the terminals of which are progressively offset. Separately tuned concentric such coils of different dimensions exhibit relatively close intercoil clearance in one dimension and relatively large clearances in the orthogonal dimension provide different flux return characteristics for selective RF coupling.
To obtain oocytes, freshly eclosed females were matured at 25°C for 3-5 d on yeasted food in the presence of males. For fixed analysis, samples were dissected in methanol and stained as previously described (Cullen and Ohkura, 2001). Under these conditions, large oocytes consisted mostly of those at stage 14 that naturally arrest in metaphase I. The following primary antibodies were used for immunostaining: anti-α-tubulin (mouse monoclonal DM1A; 1:250; Sigma-Aldrich), anti-TACC (rabbit polyclonal D-TACC-CTD; 1:1,000; Loh et al., 2012), anti-Subito (rat polyclonal; 1:250; Loh et al., 2012), anti-Msps (rabbit polyclonal; HN264; 1:250; Cullen and Ohkura, 2001), anti-Ncd (rabbit polyclonal, 1:1,000; this study), and anti-GFP (rabbit polyclonal; A11122; 1:500 for Fig. 2 or 1:250 for Fig. 5; Thermo Fisher Scientific). Cy3-, Cy5-, or Alexa Fluor 488-conjugated secondary antibodies were used (1:250-1:1,000; The Jackson Laboratory or Molecular Probes), and DNA was stained using 0.4 µg/ml DAPI ...
Purpose: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. However, up to now, quantitative estimates of this important characteristic have been limited to ex vivo ELISA essays of tissue biopsies and/or PET-based analysis. We aim at developing a novel approach in optical imaging, involving specific probes that do not interfere with the binding of the therapeutic agents, thus, excluding competition between therapy and imaging. Affibody-based molecular probes seem to be ideal for in vivo analysis of HER2 receptors using near-infrared optical imaging. To achieve quantification of HER2 receptors and overcome experimental uncertainties, we analyze post-injection temporal variations of the fluorescence intensity in the tumor area.Experimental design: Quantitative in vivo near-infrared imaging uses HER2-specific albumin-binding domain-fused-(ZHER2:342)2 Affibody molecules, labeled with AlexaFluor750 dye, as targeting agents. Subcutaneous ...
The success of new therapies hinges on our ability to understand their molecular and cellular mechanisms of action. We modified BET bromodomain inhibitors, an epigenetic-based therapy, to create functionally conserved compounds that are amenable to click chemistry and can be used as molecular probes in vitro and in vivo. We used click proteomics and click sequencing to explore the gene regulatory function of BRD4 (bromodomain containing protein 4) and the transcriptional changes induced by BET inhibitors. In our studies of mouse models of acute leukemia, we used high-resolution microscopy and flow cytometry to highlight the heterogeneity of drug activity within tumor cells located in different tissue compartments ...
BD In-Fusion™ is a trademark of BD Biosciences Clontech; Gateway® is a registered trademark of Invitrogen Life Technologies; LNA™ and Locked Nucleic Acid are trademarks of Exiqon; NASBA® is a registered trademark of bioMerieux; SYBR® is a registered trademark of Molecular Probes, Inc.; TaqMan® is a registered trademark of Roche Molecular Systems, Inc.; TOPO® is a registered trademark of Invitrogen Corporation; MLPA® is a registered trademark of MRC-Holland ...
Dear Liz: The compound that is designed for that type of experiment is called Cell Trakker Green, from Molecular Probes. You incubate that with your living cells (30 mins, incubator), then they are labeled with a fluorescent compound that is green, non-toxic and that stays within the cells for their lifespan. It also stays within the cells after fixation. (fluorescein optics on your microscope) I have labeled cells and then transferred them to another co-culture and kept them going for one week before fixing and I was able to still detect labeled cells. I am not sure about how well they will last for 3 weeks. But it was the best thing I could find for the type of thing you are describing. It is also used for transplantation experiments. Good luck! Sarah Univ. Cincinnati _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mai ...
Label organelles in live or fixed cells using Molecular Probes fluorescent stains for mitochondria, lysosomes, ER, Golgi and nuclei.
Gold nanoprobes paired with FRET (Förster resonance energy transfer) microscopy could allow researchers to study cancer cells in more minute deta
LI-COR® Biosciences has teamed up with Molecular Targeting Technologies, Inc. to offer PSVue® 794 a near-infrared fluorescent probe for researchers who have the need to detect bacteria, apoptotic and necrotic cells, or other anionic membranes for in vivo or in vitro use.
A method for the determination of nucleic acids which is highly specific and simple comprises the formation of a triple stranded binding complex including two separate, different probe molecules and detecting the formation of the complex via the inclusion of one of the probes. The method can be used to differentiate between nucleic acids having a single base difference in sequence.
The matrix metallo proteases (MMPs) are a large family of enzymes that have been found to regulate many aspects of both normal cellular biology and pathogenesis...
The genome of an infant who lived in Alaska thousands of years ago represents a previously unknown group of humans called Ancient Beringians, who share a common lineage with other Native Americans. 0 Comments. ...
Probe definition is - a slender medical instrument used especially for exploration (as of a wound or body cavity). How to use probe in a sentence. Synonym Discussion of probe.
Fill out the form below to view the Whitepaper on how a defective probe can permanently damage any System it comes into contact with. [contact-form-7 id="6751" title="White Page Killer Probe"]. by Conquest Imaging on 12.4.2017. link ...
Tempo - stainless steel 4+ inches anal probe with smooth texture by Aneros from EdenFantasys. Discreet and secure worldwide shipping.
Information about Beacons Pharmaceuticals products database; lists of Beacons Pharmaceuticals pharmaceutical drugs and health care production from Drugs-about.com
Choose Tektronix Passive Probes to accurately visualize signals over a broad range of applications. Find the right Passive Probe for your needs.
Gentaur molecular products has all kinds of products like :search , Panomics \ PO_B EMSA Probe Set \ AY1385P for more molecular products just contact us
Using a range of imaging modalities, our researchers can probe - at the molecular level - the events that underpin cellular health and dysfunction.
Post authored by FMC Communications Intern Olivia Brown]. When musicians travel with instruments, they often have to gamble with the safety of their most prized possessions. While some airlines are more understanding and allow musicians to purchase extra seats so that they can carry their instruments onboard instead of stowing them with other luggage in the cargo hold, many artists are forced to check their gear - too frequently resulting in damage. And size and weight requirements can be unpredictable.. However, this unfair practice is finally about to change. After more than a decade of lobbying from the American Federation of Musicians and others, Congress included a provision to help traveling musicians in last years legislation Federal Aviation Administration (FAA) reauthorization bill. The provision states that smaller instruments may be brought onboard as carry-on luggage, and that musicians will be allowed to purchase seats to accommodate larger instruments that are not durable enough ...
CARLSBAD, Calif., Nov. 5, 2012 /PRNewswire/ -- Life Technologies Corporation (NASDAQ: LIFE) today announced the acquisition of Advanced Microscopy Group or AMG, a privately held developer of imaging systems for research microscopy incorporated as Westover Scientific, Inc. The manufacturer of the FLoid® Cell Imaging Station currently sold by Life, AMG enables Life to expand its product line of cell imaging instrumentation, while leveraging its Molecular Probes® portfolio of fluorescent dyes and reagents.. The acquisition also provides new product development opportunities for both laboratory and portable imaging devices. Life Technologies Molecular Probes® range of fluorescent dyes and probes are broadly used in the research market and constitute a natural complement to the EVOS® range of microscopes manufactured by AMG.. "Our acquisition of Advanced Microscopy Group brings together two leaders in the cell imaging field," said Peter Dansky, president of Molecular and Cell Biology at Life ...