Affibody molecules have lately shown great potential as tools for in vivo molecular imaging. These small, 3-helical bundles, with their highly stable protein scaffold, are well suited for the often harsh conditions of radiolabeling. Their small size allows for rapid clearance from the blood circulation which permits the collection of images already within hours after injection. This thesis includes four papers aimed at engineering different variants of a HER2-binding Affibody molecule to enable effective and flexible radiolabeling and enhancing the molecular imaging in terms of imaging contrast and resolution.. In paper I an Affibody molecule was engineered to function as a multifunctional platform for site-specific labeling with different nuclides for radionuclide imaging. This was done using only natural amino acids, thereby allowing for both synthetic and recombinant production. By grafting the amino acid sequence -GSECG to the C-terminal of our model-protein, a HER2-binding Affibody ...
This thesis describes the fabrication, characterization, and application of radiolabeled multifunctional microspheres for Cerenkov luminescence imaging and targeting of angiogenesis in cancer and atherosclerosis. Cerenkov luminescence imaging has gained attention as a new type of molecular imaging modality for its potential to bridge optical imaging with nuclear imaging. The engineered microsphere design focuses on intensifying the Cerenkov luminescence emission and shifting the emission wavelengths toward the near-infrared for better penetration of light in biological tissue. This includes the use of a high-refractive index oil core that increases the intensity of Cerenkov luminescence by lowering the Cerenkov radiation energy threshold, as well as near-infrared quantum dots that can be optically excited by Cerenkov luminescence and generate higher-wavelength optical emission. In addition to signal enhancement of Cerenkov luminescence, the modification of the microsphere surface is addressed ...
Background: Cerenkov luminescence imaging (CLI) provides a great potential for clinical translation of optical molecular imaging techniques through using clinical approved radiotracers. However, it is difficult to obtain the Cerenkov luminescence signal of deeper biological tissues due to the small magnitude of the signal. To efficiently acquire the weak Cerenkov luminescence, we developed an endoscopic Cerenkov luminescence imaging (ECLI) system to reduce the in vivo imaging depth with minimum invasion, and validated the system on small animal tumor models. Methods: For the ECLI system, the laparoscope was connected to a high sensitive charge-couple device (CCD) camera (DU888+, Andor, UK) by a custom made adapter. We conducted a series of in vitro and in vivo experiments by use of the system. In the in vitro experiment, the endoscopic luminescence images of the 18F-FDG with various activities in EP tubes were acquired using ECLI system, and the sensitivity was compared with conventional CLI ...
Leong-Poi and colleagues used monoclonal antibodies against murine αv or echistatin, a viper venom disintegrin containing the well known RGD (arginine-glycine-aspartic acid) sequence [8]. Antibody-bearing and echistatin-conjugated microbubbles exhibited high potential to image activated endothelium in subcutaneously implanted Matrigel plugs enriched with fibroblast growth factor-2 (FGF-2). Microbubble retention inside the microvasculature was much higher as compared to non-targeted microbubbles, however, no significant differences in the retention of both types of targeted microbubbles were observed. Thus, monoclonal antibodies against murine αv and echistatin can be used for targeted imaging approaches.. In another study, Leong-Poi and colleagues applied echistatin-conjugated microbubbles to assess endogeneous and therapeutic arteriogenesis in a hindlimb ischemia model in rats [9]. They observed an increase in retention of integrin-binding microbubbles prior to a measurable increase in blood ...
Stabilized microbubbles with a size between 1-5 µm are used as ultrasound contrast agents in the clinical routine. They have shown convincing results for the vascular characterization of tissues as well as in echocardiography. Due to their size, mic
Visualization of specific molecular events with magnetic resonance imaging (MRI) holds great promise for both clinical and basic science applications. The ex...
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Use humanized mice as in vivo tools for mimicking human pathological conditions and diseases, and for conducting preclinical research.
CBSET is a recognized leader in preclinical research, helping sponsors around the globe accelerate the development of innovative therapeutics.
Geisinger Health Systems Neuroscience Institute is using the latest optical molecular imaging technology from Carestream Molecular Imaging as part of its cutting-edge research of brain cancer treatment.
Molecularly-targeted microbubbles (MBs) are increasingly being recognized as promising contrast agents for oncological molecular imaging with ultrasound. With the detection and validation of new molecular imaging targets, novel binding ligands are needed that bind to molecular imaging targets with high affinity and specificity. In this study we assessed a novel class of potentially clinically translatable MBs using an engineered 10th type III domain of human-fibronectin (MB-FN3VEGFR2) scaffold-ligand to image VEGFR2 on the neovasculature of cancer. The in vitro binding of MB-FN3VEGFR2 to a soluble VEGFR2 was assessed by flow-cytometry (FACS) and binding to VEGFR2-expressing cells was assessed by flow-chamber cell attachment studies under flow shear stress conditions. In vivo binding of MB-FN3VEGFR2 was tested in a transgenic mouse model (FVB/N Tg(MMTV/PyMT634Mul) of breast cancer and control litter mates with normal mammary glands. In vitro FACS and flow-chamber cell attachment studies showed ...
fluorodeoxyglucose using a sensitive CCD camera. Phantom and in vivo mouse imaging experiments have demonstrated that surface measurements of the emitted Cerenkov optical photons could be used to reconstruct the radiotracer activity distribution inside an object by modeling the optical photon propagation with the diffusion equation and reconstructing the optical emission source distribution iteratively with a preconditioned conjugate gradient method.. © 2010 Optical Society of America. Full Article , PDF Article ...
Backround Microcalcifications may provoke plaque rupture leading to acute cardiovascular events. However, the mechanisms that initiate arterial calcification remain obscure.. Methods and Results We tested the hypothesis that inflammation promotes osteogenesis in atherosclerotic plaques using in vivo molecular imaging in apoE−/− mice (n=40). A bisphosphonate-derivatized near-infrared fluorescent (NIRF) imaging agent (750 nm) visualized osteogenic activity that was otherwise undetectable by X-ray computed tomography. Flow cytometry validated target cells as osteoblasts. A spectrally distinct NIRF nanoparticle (680 nm) was coinjected to simultaneously image macrophages. Fluorescence reflectance mapping associated osteogenic activity with inflammation in aortas of apoE−/− mice. Intravital dual-channel fluorescent microscopy further monitored osteogenic changes in carotid plaques at 20 and 30 weeks of age and revealed that both macrophage burden and osteogenesis concomitantly increased during ...
Although the scientific community continues to combat male bias in preclinical research, representation of women in late-phase clinical trials has improved markedly in recent years.21 The National Institutes of Health initiative to increase the use of female organisms and cells in preclinical research ensures that the scientific community as a whole will grow to fully address the health of both men and women. A major obstacle to the incorporation of female animals into studies, however, has been the belief that the rodent estrous cycle would render the study of these animals prohibitively expensive or difficult. Our study shows for the first time in a single large data set of physiological measurements that this belief is not accurate.. Our findings show that sex differences in CV among rats are small despite hormone fluctuations because of the estrous cycle. Moreover, for the majority of phenotypes, cohorts larger than typically used in preclinical research are required to detect the sex ...
4. Developing imaging probes and methods for diabetes and obesity. In the past years, one of my research directions has been focused on the development of probes and technologies for Imaging of Metabolically Abnormal Degeneration (MAD) diseases, which include diabetes and obesity. Based on the structure of streptozotocin (STZ), a beta cell specific small molecule, we have developed two beta-cell specific NIRF probes. More recently, my research has been focused on imaging Brown Adipose Tissue (BAT), which is a re-emerged target for MAD diseases such as diabetes and obesity. However, highly selective imaging probe for BAT mass is still lack. For this project, my primary contributions include: 1) My research group discovered several NIRF probes suitable for imaging BAT mass through a top-down screening method (Manuscript under revision). 2) My group also demonstrated that Cerenkov Luminescence Imaging (CLI), a newly developed technology, could be used to image BAT with 18F-FDG. Compared to PET ...
An optical imaging technique, Cerenkov luminescence imaging, shows excellent promise as a potential new method for rapid, high-throughput screening of radiopharmaceuticals, said a study published in the July issue of the Journal of Nuclear Medicine.
Molecular geneticist Dana Tsui developed clinical test to profile circulating tumor DNA and other nucleic acids in plasma and body fluids for noninvasive molecular diagnostics.
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Research focus. My ultimate ambition is to better understand immunosenescence and immune dysregulation over the trajectory of aging, and how these changes relate to compromised health and quality of life in old age. This is particularly important since over the next several decades the world will undergo an unprecedented increase in the proportion of adults over the age of 65. Hence, strategies to promote healthy aging will be critical to minimize the social and economic burdens that are normally associated with elder care. Implicit to this goal is a continual advancement of our understanding of the biology of aging. My research focus features a comprehensive approach that combines epidemiological techniques to identify inherent and modifiable risk factors of age-related disease and morbidity, and in vitro and ex vivo molecular methods to examine potential biological mechanisms. This approach often features immune and inflammation related soluble and cell-associated biomarkers that not only ...
PubMedID: 27502060 | Noninvasive Contrast-Enhanced Ultrasound Molecular Imaging Detects Myocardial Inflammatory Response in Autoimmune Myocarditis. | Circulation. Cardiovascular imaging | 8/1/2016
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has established itself among the plethora of mass spectrometry applications. In the biomedical field, MALDI-MSI is being more frequently recognized as a new method for the discovery of biomarkers, targets of treatment, classification of diseased and healthy tissues or to predict the outcome of a pathology. The technology has been used to study the localization of proteolytic peptides directly on tissue sections. A direct correlation between the detected peptides and the distribution and identity of the original precursor protein is the ultimate goal of any MALDI-MSI experiment. Enzymatic digestion protocols are commonly used to reveal the protein signature of these complex tissues. Considerations that pertain to methods of sample preparation, on-tissue digestion, data analysis and visualization will be addressed. This review will also discuss selected applications of on-tissue digestion combined with the MALDI-MSI ...
ROCKVILLE, Md.--(BUSINESS WIRE)-- Rexahn Pharmaceuticals, Inc. (NYSE Amex: RNN), a clinical stage pharmaceutical company developing and commercializing potential best in class oncology and CNS
Preclinical CRO for pharmacology, dermatology and cosmetics, in vitro models and bioassays alternative methods to animal testing.
Triad Hospitals Inc., Perot Systems Corp. and McKesson Corp. announced a $1.3 billion healthcare information technology initiative to support Triads clinical excellence and business transformation programs.GE Healthcare and PhotoCure ASA signed a licensing agreement that grants GE exclusive global rights outside of the U.S. and the Nordic region to market and distribute PhotoCures product Hexvix (hexaminolevulinate), an optical molecular imaging agent for bladder cancer.
Researchers at the Stanford University School of Medicine have developed a molecular probe that sets aglow tumor cells within living animals. Their goal is to use the probe to improve the diagnosis and treatment of cancer and other diseases.
... | MD Biosciences insights discussing preclinical research in CNS, inflammation and metabolic drug discovery research.
HCS Pharma is a biotechnological startup focused on in vitro preclinical research development with a specialisation in cellular imagery : High Content Analysis (HCA) and High Content Screening (HCS ...
HCS Pharma is a biotechnological startup focused on in vitro preclinical research development with a specialisation in cellular imagery : High Content Analysis (HCA) and High Content Screening (HCS ...
As an optical molecular imaging modality, fluorescence molecular tomography (FMT) can monitor the activities of organisms in vivo at the molecular and cellular levels. However, the recovered image...
In this study, we demonstrated the effects of the molecular weight (MW) of a green-solvent processable semiconducting polymer (asy-PBTBDT) on its photovoltaic performance and device thermal stability in green processed devices for the first time. The asy-PBTBDT with a high MW (132 kDa) had the highest μh values (4. 2018 Journal of Materials Chemistry A HOT Papers
intentions, hopes, beliefs, expectations, representations, projections, plans or predictions of the future are forward-looking statements within the meaning of the Private Securities Litigation Reform Act of 1995. The forward-looking statements involve risks and uncertainties including, but not limited to, the risk that the data from these preclinical studies will not be duplicated in future clinical trials and the risk that the companys clinical development strategy will not generate clinical data sufficiently compelling to attract a partner to advance the program. The companys actual results could differ materially from those in any such forward-looking statements. Factors that could cause actual results to differ materially include, but are not limited to, uncertainties associated with completing preclinical and clinical trials for our technologies; the early stage of product development; the significant costs to develop our products as all of our products are currently in development, ...
Palma, Spain and San Diego, USA, August 6, 2018 - Laboratoris Sanifit S.L., a clinical-stage biopharmaceutical company focused on treatments for calcification disorders, today announced the completion of enrollment of its Phase 2b CaLIPSO clinical trial investigating Sanifit’s lead candidate SNF472, for the tr...
Superresolution imaging is a rapidly emerging new field of microscopy that dramatically improves the spatial resolution of light microscopy by over an order of magnitude (approximately 10-20-nm resolution), allowing biological processes to be described at the molecular scale. Here, we discuss a form …
The activities of the PI node are closely related to the Preclinical Imaging Laboratory (PIL). PIL focuses on the application of sophisticated imaging techniques in experimental systems and small experimental animals. The panel of available techniques include micro-MR, micro-CT, micro-SPECT, micro-PET, micro-ultrasound and various optical imaging procedures (bioluminescence, fluorescence) as well as options for the combination of these methods (hybrid technologies). Complimentary, for precise induction of tissue and organ alterations, a dedicated small animal irradiation device is available. The PIL is closely and intensely cooperating with the Department of Biomedical Imaging and Image guided Therapy in order to instantly exploit the preclinical research into clinical applications. ...
Turku BioImaging offers its researchers a cost-effective possibility to versatile biomedical imaging. The crown jewel of the centre is a brand new super-resolution STED microscope which is the only one in Finland. All operations aim to bring the valuable equipment to as efficient as possible public use.
This makes it possible to measure samples quickly and adapt test conditions immediately during an experiment instead of evaluating them afterwards," says Rainer Heintzmann, describing the practical benefits of the new technology.. The scientists tested the method on biological cells and recorded the movements of mitochondria, the energy centres of the cells that are about one micrometer in size. "We were able to produce about 60 frames per second - a higher frame rate than those of motion pictures. The time between measurement and image is less than 250 milliseconds, which is why the technology allows real-time recordings," says Andreas Markwirth.. So far, superresolution images have often been combined with conventional methods: A conventional fast microscope is used to first find structures. These structures can then be examined in detail using a superresolution microscope. "However, some structures are so small that they cannot be found with conventional microscopes, for example special pores ...
Egg: This egg has only been seen on two DNA confirmed occasions, but was easily recognised by its red chorion. It was only confused with CDIA1 when that species lacked a furry chorion, and HIA2. Incubation is at least 10 days (23 C).. Larva description: The newly hatched larva has lightly pigmented eyes, with long dorsal and pelvic fin elements (D). The larva resembles other lampriformes, but instead of a single dorsal fin thread with leaflike expansions as seen in Regalecus (CDIA1) and Zu cristatus larvae ( HIA2), has 4 elongate dorsal spines, and long threadlike extensions to the pelvic fins. D: 2 days PH, 14 days post collection; E: NH 13 days post collection. Four larvae, hatched from eggs, have been barcoded, submitted under my egg codes CDIA1, CDIA1A and HIA2 (2x). They provided a match with adult fish identified as Lampris guttatus, from Australia (BOLD). The barcode of an adult collected off Durban and identified as L. guttatus (TZMSA442-04,ADC117.1-1 in BOLD), does not match these four ...
Background Optical imaging (OI) techniques such as bioluminescence and fluorescence imaging have been widely used to track diseases in a non-invasive manner within living subjects. These techniques generally require bioluminescent and fluorescent probes. Here we demonstrate the feasibility of using radioactive probes for in vivo molecular OI. Methodology/Principal Findings By taking the advantages of low energy window of light (1.2-3.1 eV, 400-1000 nm) resulting from radiation, radionuclides that emit charged particles such as β+ and β− can be successfully imaged with an OI instrument. In vivo optical images can be obtained for several radioactive probes including 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG), Na18F, Na131I, 90YCl3 and a 90Y labeled peptide that specifically target tumors. Conclusions/Significance These studies demonstrate generalizability of radioactive OI technique. It provides a new molecular imaging strategy and will likely have significant impact on both small animal and
In-vivo optical molecular imaging methods for producing an image of an animal are described. A time series of image data sets of an optical contrast substance in the animal is acquired using an optical detector Each image data set is obtained at a selected time and has the same plurality of pixels, with each pixel having an associated value. The image data sets are analyzed to identify a plurality of distinctive time courses, and respective pixel sets are determined from the plurality of pixels which correspond to each of the time courses. In one embodiment, each pixel set is associated with an identified anatomical or other structure, and an anatomical image map of the animal can be generated which includes one or more of the anatomical structures.
The Test Is Based On The Principle Of Sandwich Immunoassay For Determination Of CEA In Serum. Monoclonal And Polyclonal Antibodies Are Employed To Identify CEA Specifically. This One Step Test Is Very...
Asterand Bioscience supports human drug discovery by providing high quality, well characterized human biomaterials and preclinical research services. The benefits of using Asterand Bioscience are Validated native human targets, early drug attrition, advance promising compounds faster, fewer development failures, and reduced development costs.
Expert in biotechnology and pharmacology, provide consulting services in the area of preclinical research, RNAi, pharm/tox studies for IND. List of peer-review publications, patents, and contact information. ...
The event will run over two full days, with presentations from the leading researchers and solution providers, covering bioimaging techniques applied at the molecular, organelle, cellular and tissue level. There will be a Panel Discussion at the end of Day Two to discuss the topics raised throughout the meeting. In addition to the comprehensive speaker programme we will also have two lunchtime Company Workshops hosted by Sciencewerke, Ibidi and Phasics. These are free for all attendees and run from 13:15 to 14:00 on both days ...
Molecular Probes Cell Imaging Guide with Protocols brbrThis mobile app is designed to help you find fluorescent dyes reagents and protocols for cell biology related fluorescence microscopy applications brbrMolecular Probes mobile app
Lastek is the exclusive distributor in Australia and New Zealand for more than fifty leading international laser, optic, detection, spectroscopy and imaging manufacturers.
Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
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Technology Networks is an internationally recognised publisher that provides access to the latest scientific news, products, research, videos and posters.
Hot‐start PCR is a technique that improves PCR performance by reducing nonspecific amplification during the initial setup stages of the PCR
June 23, 2014 - Researchers have announced the results of a study that used contrast ultrasound molecular imaging (CMI) to examine a relatively new role for platelets at early to mid stages of atherosclerosis. Atherosclerosis, or hardening of the arteries due to plaque buildup, can lead to many serious health problems, including stroke and coronary heart disease.. "It is well known that platelets, which are a constituent of blood clots, play a role in forming large clots that occlude arteries in stroke and heart attack. Our study opens up a whole new perspective on how platelets play a role in provoking the earlier initiation and growth of atherosclerotic plaques," said primary investigator Chi Young Shim, M.D., Ph.D. Shim is a postdoctoral researcher at Oregon Health & Science University in Portland, Ore.; she and her colleagues used molecular imaging with "smart" targeted microbubbles to detect the sticking of platelets to the vessel wall at various stages of atherosclerosis. Jonathan Lindner, ...