Affibody molecules have lately shown great potential as tools for in vivo molecular imaging. These small, 3-helical bundles, with their highly stable protein scaffold, are well suited for the often harsh conditions of radiolabeling. Their small size allows for rapid clearance from the blood circulation which permits the collection of images already within hours after injection. This thesis includes four papers aimed at engineering different variants of a HER2-binding Affibody molecule to enable effective and flexible radiolabeling and enhancing the molecular imaging in terms of imaging contrast and resolution.. In paper I an Affibody molecule was engineered to function as a multifunctional platform for site-specific labeling with different nuclides for radionuclide imaging. This was done using only natural amino acids, thereby allowing for both synthetic and recombinant production. By grafting the amino acid sequence -GSECG to the C-terminal of our model-protein, a HER2-binding Affibody ...
This thesis describes the fabrication, characterization, and application of radiolabeled multifunctional microspheres for Cerenkov luminescence imaging and targeting of angiogenesis in cancer and atherosclerosis. Cerenkov luminescence imaging has gained attention as a new type of molecular imaging modality for its potential to bridge optical imaging with nuclear imaging. The engineered microsphere design focuses on intensifying the Cerenkov luminescence emission and shifting the emission wavelengths toward the near-infrared for better penetration of light in biological tissue. This includes the use of a high-refractive index oil core that increases the intensity of Cerenkov luminescence by lowering the Cerenkov radiation energy threshold, as well as near-infrared quantum dots that can be optically excited by Cerenkov luminescence and generate higher-wavelength optical emission. In addition to signal enhancement of Cerenkov luminescence, the modification of the microsphere surface is addressed ...
TY - JOUR. T1 - Cerenkov luminescence imaging of αvβ6 integrin expressing tumors using 90Y-labeled peptides. AU - Satpati, Drishty. AU - Hausner, Sven H.. AU - Bauer, Nadine. AU - Sutcliffe, Julie. PY - 2014. Y1 - 2014. N2 - Cerenkov luminescence imaging (CLI) is an emerging preclinical molecular imaging modality that tracks the radiation emitted in the visible spectrum by fast moving charged decay products of radionuclides. The aim of this study was in vitro and in vivo evaluation of the two radiotracers, 90Y-DOTA- PEG28-A20FMDV2 (90Y-1) and 90Y-DOTA-Ahx- A20FMDV2 (90Y-2) (,99% radiochemical purity, 3.7 GBq/μmol specific activity) for noninvasive assessment of tumors expressing the integrin αvβ6 and their future use in tumor targeted radiotherapy. Cell binding and internalization in αvβ 6-positive cells was 90Y-1: 10.1 ± 0.8%, 50.3 ± 2.1%; 90Y-2: 22.4 ± 1.7%, 44.7 ± 1.5% with ,5% binding to αvβ6-negative control cells. Biodistribution studies showed maximum αvβ6- positive tumor ...
Background: Cerenkov luminescence imaging (CLI) provides a great potential for clinical translation of optical molecular imaging techniques through using clinical approved radiotracers. However, it is difficult to obtain the Cerenkov luminescence signal of deeper biological tissues due to the small magnitude of the signal. To efficiently acquire the weak Cerenkov luminescence, we developed an endoscopic Cerenkov luminescence imaging (ECLI) system to reduce the in vivo imaging depth with minimum invasion, and validated the system on small animal tumor models. Methods: For the ECLI system, the laparoscope was connected to a high sensitive charge-couple device (CCD) camera (DU888+, Andor, UK) by a custom made adapter. We conducted a series of in vitro and in vivo experiments by use of the system. In the in vitro experiment, the endoscopic luminescence images of the 18F-FDG with various activities in EP tubes were acquired using ECLI system, and the sensitivity was compared with conventional CLI ...
Leong-Poi and colleagues used monoclonal antibodies against murine αv or echistatin, a viper venom disintegrin containing the well known RGD (arginine-glycine-aspartic acid) sequence [8]. Antibody-bearing and echistatin-conjugated microbubbles exhibited high potential to image activated endothelium in subcutaneously implanted Matrigel plugs enriched with fibroblast growth factor-2 (FGF-2). Microbubble retention inside the microvasculature was much higher as compared to non-targeted microbubbles, however, no significant differences in the retention of both types of targeted microbubbles were observed. Thus, monoclonal antibodies against murine αv and echistatin can be used for targeted imaging approaches.. In another study, Leong-Poi and colleagues applied echistatin-conjugated microbubbles to assess endogeneous and therapeutic arteriogenesis in a hindlimb ischemia model in rats [9]. They observed an increase in retention of integrin-binding microbubbles prior to a measurable increase in blood ...
Stabilized microbubbles with a size between 1-5 µm are used as ultrasound contrast agents in the clinical routine. They have shown convincing results for the vascular characterization of tissues as well as in echocardiography. Due to their size, mic
Visualization of specific molecular events with magnetic resonance imaging (MRI) holds great promise for both clinical and basic science applications. The ex...
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In vivo imaging is at the heart of preclinical research into cancer biology and treatment, with advances in technology delivering tools that are ever more powerful, accurate and easy-to-use.
Use humanized mice as in vivo tools for mimicking human pathological conditions and diseases, and for conducting preclinical research.
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TY - JOUR. T1 - Sugar-based biopolymers as novel imaging agents for molecular magnetic resonance imaging. AU - Han, Zheng. AU - Liu, Guanshu. PY - 2019/7/1. Y1 - 2019/7/1. N2 - Sugar-based biopolymers have been recognized as attractive materials to develop macromolecule- and nanoparticle-based cancer imaging and therapy. However, traditional biopolymer-based imaging approaches rely on the use of synthetic or isotopic labeling, and because of it, clinical translation often is hindered. Recently, a novel magnetic resonance imaging (MRI) technology, chemical exchange saturation transfer (CEST), has emerged, which allows the exploitation of sugar-based biopolymers as MRI agents by their hydroxyl protons-rich nature. In the study, we reviewed recent studies on the topic of CEST MRI detection of sugar-based biopolymers. The CEST MRI property of each biopolymer was briefly introduced, followed by the pre-clinical and clinical applications. The findings of these preliminary studies imply the enormous ...
Geisinger Health Systems Neuroscience Institute is using the latest optical molecular imaging technology from Carestream Molecular Imaging as part of its cutting-edge research of brain cancer treatment.
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Lundbeck divests preclinical research programs Two programs outside Lundbecks areas of focus are transferred to MindImmune Therapeutics, Inc. H. Lundbeck A/S (Lundbeck) further focuses its preclinical research pipeline with the divestment of two research programs to biotech company MindImmune Therapeutics, Inc. (MindImmune). In exchange for the programs, Lundbeck receives an equity interest in MindImmune as well as milestone payments and royalties according to the progression of the programs. The agreement follows Lundbecks strategy of focusing its efforts within four disease areas; depression, schizophrenia, Alzheimers disease and Parkinsons disease, for which the programs are not relevant. Per its strategy Lundbeck itself has therefore not prioritized further development of the programs, but the agreement ensures that these potential new treatments will now be brought forward. There will be no costs for Lundbeck associated with the future development of the programs. We focus on ...
Molecularly-targeted microbubbles (MBs) are increasingly being recognized as promising contrast agents for oncological molecular imaging with ultrasound. With the detection and validation of new molecular imaging targets, novel binding ligands are needed that bind to molecular imaging targets with high affinity and specificity. In this study we assessed a novel class of potentially clinically translatable MBs using an engineered 10th type III domain of human-fibronectin (MB-FN3VEGFR2) scaffold-ligand to image VEGFR2 on the neovasculature of cancer. The in vitro binding of MB-FN3VEGFR2 to a soluble VEGFR2 was assessed by flow-cytometry (FACS) and binding to VEGFR2-expressing cells was assessed by flow-chamber cell attachment studies under flow shear stress conditions. In vivo binding of MB-FN3VEGFR2 was tested in a transgenic mouse model (FVB/N Tg(MMTV/PyMT634Mul) of breast cancer and control litter mates with normal mammary glands. In vitro FACS and flow-chamber cell attachment studies showed ...
fluorodeoxyglucose using a sensitive CCD camera. Phantom and in vivo mouse imaging experiments have demonstrated that surface measurements of the emitted Cerenkov optical photons could be used to reconstruct the radiotracer activity distribution inside an object by modeling the optical photon propagation with the diffusion equation and reconstructing the optical emission source distribution iteratively with a preconditioned conjugate gradient method.. © 2010 Optical Society of America. Full Article , PDF Article ...
Backround Microcalcifications may provoke plaque rupture leading to acute cardiovascular events. However, the mechanisms that initiate arterial calcification remain obscure.. Methods and Results We tested the hypothesis that inflammation promotes osteogenesis in atherosclerotic plaques using in vivo molecular imaging in apoE−/− mice (n=40). A bisphosphonate-derivatized near-infrared fluorescent (NIRF) imaging agent (750 nm) visualized osteogenic activity that was otherwise undetectable by X-ray computed tomography. Flow cytometry validated target cells as osteoblasts. A spectrally distinct NIRF nanoparticle (680 nm) was coinjected to simultaneously image macrophages. Fluorescence reflectance mapping associated osteogenic activity with inflammation in aortas of apoE−/− mice. Intravital dual-channel fluorescent microscopy further monitored osteogenic changes in carotid plaques at 20 and 30 weeks of age and revealed that both macrophage burden and osteogenesis concomitantly increased during ...
Although the scientific community continues to combat male bias in preclinical research, representation of women in late-phase clinical trials has improved markedly in recent years.21 The National Institutes of Health initiative to increase the use of female organisms and cells in preclinical research ensures that the scientific community as a whole will grow to fully address the health of both men and women. A major obstacle to the incorporation of female animals into studies, however, has been the belief that the rodent estrous cycle would render the study of these animals prohibitively expensive or difficult. Our study shows for the first time in a single large data set of physiological measurements that this belief is not accurate.. Our findings show that sex differences in CV among rats are small despite hormone fluctuations because of the estrous cycle. Moreover, for the majority of phenotypes, cohorts larger than typically used in preclinical research are required to detect the sex ...
4. Developing imaging probes and methods for diabetes and obesity. In the past years, one of my research directions has been focused on the development of probes and technologies for Imaging of Metabolically Abnormal Degeneration (MAD) diseases, which include diabetes and obesity. Based on the structure of streptozotocin (STZ), a beta cell specific small molecule, we have developed two beta-cell specific NIRF probes. More recently, my research has been focused on imaging Brown Adipose Tissue (BAT), which is a re-emerged target for MAD diseases such as diabetes and obesity. However, highly selective imaging probe for BAT mass is still lack. For this project, my primary contributions include: 1) My research group discovered several NIRF probes suitable for imaging BAT mass through a top-down screening method (Manuscript under revision). 2) My group also demonstrated that Cerenkov Luminescence Imaging (CLI), a newly developed technology, could be used to image BAT with 18F-FDG. Compared to PET ...
Bioimaging is a term that covers the complex chain of acquiring, processing and visualizing structural or functional images of living objects or systems, including extraction and processing of image-related information. Examples of image modalities used in bioimaging are many, including: X-ray, CT, MRI and fMRI, PET and HRRT PET, SPECT, MEG and so on. Medical imaging and microscope/fluorescence image processing are important parts of bioimaging referring to the techniques and processes used to create images of the human body, anatomical areas, tissues, and so on, down to the molecular level, for clinical purposes, seeking to reveal, diagnose, or examine diseases, or medical science, including the study of normal anatomy and physiology. Image processing methods, such as denoising, segmentation, deconvolution and registration methods, feature recognition and classification represent an indispensable part of bioimaging, as well as related data analysis and statistical tools ...
An optical imaging technique, Cerenkov luminescence imaging, shows excellent promise as a potential new method for rapid, high-throughput screening of radiopharmaceuticals, said a study published in the July issue of the Journal of Nuclear Medicine.
Molecular geneticist Dana Tsui developed clinical test to profile circulating tumor DNA and other nucleic acids in plasma and body fluids for noninvasive molecular diagnostics.
Yecuris FRG® KO mice offer a powerful advantage over other humanized mouse models. The platform can be transplanted with cells from any donor or species to yield an infinite number of phenotypic model variants. Weve worked with hundreds of clients for over a decade to incorporate normal, super, and mutant donors for drug metabolism, NASH, metabolic disease, and infectious disease. Whether youre looking to bridge data from previous experiments to a human-relevant model, or looking to create your own novel model, the FRG® KO platform is the ideal solution. ...
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Adrian Smith discusses the role that the PREPARE Guidelines and Website play in improving the robustness and translatability of animal studies.
Research focus. My ultimate ambition is to better understand immunosenescence and immune dysregulation over the trajectory of aging, and how these changes relate to compromised health and quality of life in old age. This is particularly important since over the next several decades the world will undergo an unprecedented increase in the proportion of adults over the age of 65. Hence, strategies to promote healthy aging will be critical to minimize the social and economic burdens that are normally associated with elder care. Implicit to this goal is a continual advancement of our understanding of the biology of aging. My research focus features a comprehensive approach that combines epidemiological techniques to identify inherent and modifiable risk factors of age-related disease and morbidity, and in vitro and ex vivo molecular methods to examine potential biological mechanisms. This approach often features immune and inflammation related soluble and cell-associated biomarkers that not only ...
PubMedID: 27502060 | Noninvasive Contrast-Enhanced Ultrasound Molecular Imaging Detects Myocardial Inflammatory Response in Autoimmune Myocarditis. | Circulation. Cardiovascular imaging | 8/1/2016
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has established itself among the plethora of mass spectrometry applications. In the biomedical field, MALDI-MSI is being more frequently recognized as a new method for the discovery of biomarkers, targets of treatment, classification of diseased and healthy tissues or to predict the outcome of a pathology. The technology has been used to study the localization of proteolytic peptides directly on tissue sections. A direct correlation between the detected peptides and the distribution and identity of the original precursor protein is the ultimate goal of any MALDI-MSI experiment. Enzymatic digestion protocols are commonly used to reveal the protein signature of these complex tissues. Considerations that pertain to methods of sample preparation, on-tissue digestion, data analysis and visualization will be addressed. This review will also discuss selected applications of on-tissue digestion combined with the MALDI-MSI ...
The sulfation pathway has been shown experimentally to involve CYP2A6 aryl epoxide intermediates that are then converted to many unique glutathione products though GST enzymes. The chimeric animals demonstrate many of these conjugates both in wild-type and humanized strains.. Over the previous three year, the staff at Yecuris have run multiple experiments using lamotrigine as a probe substrate and have aggregated the data for illustrative purposes. The first sets of experiments involved the examination of the non-repopulated models (muFRG) to better understand what the baseline state of the models are. Wild-type C57Bl/6 and NOD male and female mice we compared against RG and FRG KO mice on the same strains and genders. No substantive differences were observed between any of the strains or genders, though we note that in other experiments with different compounds the immune deficient strains have demonstrated differences in physiological effect as compared to the wild-type controls. Here we ...
ROCKVILLE, Md.--(BUSINESS WIRE)-- Rexahn Pharmaceuticals, Inc. (NYSE Amex: RNN), a clinical stage pharmaceutical company developing and commercializing potential best in class oncology and CNS
The Minister of Science, Education and Sports, Dr. Radovan Fuchs formally opened the Centre for Nuclear Molecular Diagnostics at the RBI today. This is...
Preclinical CRO for pharmacology, dermatology and cosmetics, in vitro models and bioassays alternative methods to animal testing.
Triad Hospitals Inc., Perot Systems Corp. and McKesson Corp. announced a $1.3 billion healthcare information technology initiative to support Triads clinical excellence and business transformation programs.GE Healthcare and PhotoCure ASA signed a licensing agreement that grants GE exclusive global rights outside of the U.S. and the Nordic region to market and distribute PhotoCures product Hexvix (hexaminolevulinate), an optical molecular imaging agent for bladder cancer.
Researchers at the Stanford University School of Medicine have developed a molecular probe that sets aglow tumor cells within living animals. Their goal is to use the probe to improve the diagnosis and treatment of cancer and other diseases.
The Bureau also leaves open the possibility of engaging in a future rulemaking to further define the abusiveness standard.. Last year, the Bureau held a Symposium on Abusive Acts or Practices with academics and practitioners. These experts provided a variety of perspectives on the need and benefits in developing a clearer understanding of the abusiveness standard; most agreed that the Bureau should seek to resolve the uncertainty. The symposium, along with other feedback from stakeholders, was an important part of the process leading to the Bureaus decision to issue the policy statement.. ...
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Inflammation | MD Biosciences insights discussing preclinical research in CNS, inflammation and metabolic drug discovery research.
HCS Pharma is a biotechnological startup focused on in vitro preclinical research development with a specialisation in cellular imagery : High Content Analysis (HCA) and High Content Screening (HCS ...
HCS Pharma is a biotechnological startup focused on in vitro preclinical research development with a specialisation in cellular imagery : High Content Analysis (HCA) and High Content Screening (HCS ...
UROSPHERES Women Health portfolio includes both in vitro assays and in vivo models that can be used at various stages of preclinical research for the evaluation of drug candidates.. Download our model specifications below.. ...
IRDye 680LT Protein Labeling Kits can be used to label antibodies and other proteins for applications such as Western blots and microscopy.. ...
The main goal of the LBNL integrated biological imaging center is to integrate the amazing breadth of imaging expertise and technology currently at the Lab to take better advantage of our capabilities and to address some of the current limitations in performing integrated imaging, analysis and visualization.
Researchers today announced the creation of an imaging technology more powerful than anything that has existed before, and is fast enough to observe life processes as they actually happen at the molecular level.
As an optical molecular imaging modality, fluorescence molecular tomography (FMT) can monitor the activities of organisms in vivo at the molecular and cellular levels. However, the recovered image...
In this study, we demonstrated the effects of the molecular weight (MW) of a green-solvent processable semiconducting polymer (asy-PBTBDT) on its photovoltaic performance and device thermal stability in green processed devices for the first time. The asy-PBTBDT with a high MW (132 kDa) had the highest μh values (4. 2018 Journal of Materials Chemistry A HOT Papers
Cardiovascular Calcification And Bone Mineralization è un libro di Aikawa Elena (Curatore), Hutcheson Joshua D. (Curatore) edito da Humana a luglio 2021 - EAN 9783030467272: puoi acquistarlo sul sito HOEPLI.it, la grande libreria online.
intentions, hopes, beliefs, expectations, representations, projections, plans or predictions of the future are forward-looking statements within the meaning of the Private Securities Litigation Reform Act of 1995. The forward-looking statements involve risks and uncertainties including, but not limited to, the risk that the data from these preclinical studies will not be duplicated in future clinical trials and the risk that the companys clinical development strategy will not generate clinical data sufficiently compelling to attract a partner to advance the program. The companys actual results could differ materially from those in any such forward-looking statements. Factors that could cause actual results to differ materially include, but are not limited to, uncertainties associated with completing preclinical and clinical trials for our technologies; the early stage of product development; the significant costs to develop our products as all of our products are currently in development, ...
Palma, Spain and San Diego, USA, August 6, 2018 - Laboratoris Sanifit S.L., a clinical-stage biopharmaceutical company focused on treatments for calcification disorders, today announced the completion of enrollment of its Phase 2b CaLIPSO clinical trial investigating Sanifit’s lead candidate SNF472, for the tr...
Superresolution imaging is a rapidly emerging new field of microscopy that dramatically improves the spatial resolution of light microscopy by over an order of magnitude (approximately 10-20-nm resolution), allowing biological processes to be described at the molecular scale. Here, we discuss a form …
The activities of the PI node are closely related to the Preclinical Imaging Laboratory (PIL). PIL focuses on the application of sophisticated imaging techniques in experimental systems and small experimental animals. The panel of available techniques include micro-MR, micro-CT, micro-SPECT, micro-PET, micro-ultrasound and various optical imaging procedures (bioluminescence, fluorescence) as well as options for the combination of these methods (hybrid technologies). Complimentary, for precise induction of tissue and organ alterations, a dedicated small animal irradiation device is available. The PIL is closely and intensely cooperating with the Department of Biomedical Imaging and Image guided Therapy in order to instantly exploit the preclinical research into clinical applications. ...
Turku BioImaging offers its researchers a cost-effective possibility to versatile biomedical imaging. The crown jewel of the centre is a brand new super-resolution STED microscope which is the only one in Finland. All operations aim to bring the valuable equipment to as efficient as possible public use.
OmniVision Technologies, Inc., a leading developer of advanced digital imaging solutions, and Almalence Inc., one of the worlds leaders in computatio