Terpenoids, also known as isoprenoids, are a large class of natural products consisting of isoprene (C5) units. There are two biosynthetic pathways, the mevalonate pathway [MD:M00095] and the non-mevalonate pathway or the MEP/DOXP pathway [MD:M00096], for the terpenoid building blocks: isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). The action of prenyltransferases then generates higher-order building blocks: geranyl diphosphate (GPP), farsenyl diphosphate (FPP), and geranylgeranyl diphosphate (GGPP), which are the precursors of monoterpenoids (C10), sesquiterpenoids (C15), and diterpenoids (C20), respectively. Condensation of these building blocks gives rise to the precursors of sterols (C30) and carotenoids (C40). The MEP/DOXP pathway is absent in higher animals and fungi, but in green plants the MEP/DOXP and mevalonate pathways co-exist in separate cellular compartments. The MEP/DOXP pathway, operating in the plastids, is responsible for the formation of essential oil ...

Mobiluncus is a genus of Gram-negative, anaerobic, rod-shaped bacteria. While this species possesses a cell wall with structural similarity to other Gram-positive cell walls, upon Gram stain, these bacteria may be stained either Gram-negative or Gram-variable. These organisms are found in the human vagina, particularly in association with Gardnerella vaginalis in cases of bacterial vaginosis. Medical Microbiology 5th Edition, Patrick R. Murray, PhD Clark, Natalie; Tal, Reshef; Sharma, Harsha; Segars, James (2014). Microbiota and Pelvic Inflammatory Disease. Seminars in Reproductive Medicine. 32 (01): 043-049. doi:10.1055/s-0033-1361822. ISSN 1526-8004. PMC 4148456 . PMID 24390920. Schwebke JR, Lawing LF (April 2001). Prevalence of Mobiluncus spp among women with and without bacterial vaginosis as detected by polymerase chain reaction. Sex Transm Dis. 28 (4): 195-9. doi:10.1097/00007435-200104000-00002. PMID 11318249 ...

Mobiluncus mulieris ATCC ® 35240D-5™ Designation: Genomic DNA from Mobiluncus mulieris strain BV 64-5 TypeStrain=False Application:

ID D6ZJW0_MOBCV Unreviewed; 483 AA. AC D6ZJW0; DT 10-AUG-2010, integrated into UniProtKB/TrEMBL. DT 10-AUG-2010, sequence version 1. DT 25-OCT-2017, entry version 50. DE RecName: Full=Chromosomal replication initiator protein DnaA {ECO:0000256,HAMAP-Rule:MF_00377, ECO:0000256,RuleBase:RU000577}; GN Name=dnaA {ECO:0000256,HAMAP-Rule:MF_00377, GN ECO:0000313,EMBL:ADI67009.1}; GN OrderedLocusNames=HMPREF0573_10690 {ECO:0000313,EMBL:ADI67009.1}; OS Mobiluncus curtisii (strain ATCC 43063 / DSM 2711 / V125) (Falcivibrio OS vaginalis). OC Bacteria; Actinobacteria; Actinomycetales; Actinomycetaceae; OC Mobiluncus. OX NCBI_TaxID=548479 {ECO:0000313,EMBL:ADI67009.1, ECO:0000313,Proteomes:UP000006742}; RN [1] {ECO:0000313,Proteomes:UP000006742} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 43063 / DSM 2711 / V125 RC {ECO:0000313,Proteomes:UP000006742}; RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., RA Zhang L., Thornton R., Coyle M., Francisco L., Jackson L., Javaid ...

Hi All - Found this Sterile Liquid Culture Media on eBay. Says it will Multiply Spore Syringe up to 40x. for mushroom spores. I was thinking about getting some and using it with Panaeolus (Copelandia)

The TEL-AML1 fusion not only characterizes the most frequent genetic rearrangement in initial childhood ALL (20-25%) but its presence has also been associated with a favorable prognosis (9, 10, 11 , 28 , 32) . In clinical studies on initial ALL, probability of event-free survival (EFS) at 4 years was as high as 90-100% for TEL-AML1+ patients (9 , 10 , 32) . These results certainly do not represent final outcome considering that, regardless of the different prevalence of TEL-AML1 positivity at relapse of BCP-ALL (range, 3- 28%), TEL-AML1+ leukemia is biologically characterized by a long duration of first CR and that the majority of relapses (80%) occur off-therapy (median, 46 months; range, 13-125 months; Refs. 18, 19, 20, 21, 22, 23 , 33 ). The prevalence of TEL-AML1 positivity in our ongoing prospective study on first relapse of BCP-ALL is ∼17% (31 of 178 children; 33 ).. Obviously, the predictive value of TEL-AML1 positivity alone is insufficient to stratify patients to appropriate treatment ...

The ability to profile epigenomic features in single cells is facilitating the study of the variation in transcription regulation at the single cell level. Single cell methods have also facilitated the generation of cell-type resolved transcriptomic and epigenetic profiles of lineages derived ...

The company showed that its chip can detect SNPs in models of sickle-cell disease and amyotrophic lateral sclerosis, without the need for DNA amplification.

Teknova offers custom molecular biology media such as buffers and reagents, pre-poured agar plates, dry culture media, liquid culture media, and media supplements.

Teknova offers custom molecular biology media such as buffers and reagents, pre-poured agar plates, dry culture media, liquid culture media, and media supplements.

Its a multi-omic characterization of the state of the human immune system. Its a database were building based on results from expression arrays, highly multiplex flow cytometry and cytokine assays, genetic analyses, and functional studies. Were working on this internally across many institutes, we are seeking collaborations with companies, and were in touch with external investigators nationally and internationally.. Right now were looking at how the normal human immunome is perturbed by flu vaccines in nearly 200 individuals. We get two prevaccination bleeds and then we do a series of early and late post-vaccine bleeds. We hope to get about 17,000 different sub-phenotype possibilities from the flow analyses and 60-100 cytokine measurements on every one of the samples. Well keep material on reserve for later to do SNP chips, HLA typing, and eventually sequencing if it becomes cheap enough. The idea is to then put this all together in a very deep analysis.. What might it tell us?. Far more ...

Genomic and radiomic data integration, namely radiogenomics, can provide meaningful knowledge in cancer diagnosis, prognosis and treatment. Despite several data structures based on multi-layer architecture proposed to combine multi-omic biological information, none of these has been designed and assessed to include radiomic data as well. To meet this need, we propose to use the MultiAssayExperiment (MAE), an R package that provides data structures and methods for manipulating and integrating multi-assay experiments, as a suitable tool to manage radiogenomic experiment data. To this aim, we first examine the role of radiogenomics in cancer phenotype definition, then the current state of radiogenomics data integration in public repository and, finally, challenges and limitations of including radiomics in MAE, designing an extended framework and showing its application on a case study from the TCGA-TCIA archives. Radiomic and genomic data from 91 patients have been successfully integrated in a single MAE

In this application note, we describe a multi-omic approach to reveal new molecular factors involved in pathogenesis of INS with potential diagnostic and therapeutic significance.

2017: Creació de laboratori de Recerca translacional en microambient tumoral. LOBSTER: An environment to design bioimage analysis workflows for large and complex fluorescence microscopy data.. Tosi S, Bardia L, Filgueira MJ, Calon A, Colombelli J.. Bioinformatics. 2019 Dec 20. pii: btz945. doi: 10.1093/bioinformatics/btz945.. A multi-omic analysis for prospective patient stratification in localised colorectal cancer.. Tarazona Llavero N, Fontana E, Gambardella V, Gimeno-Valiente F, Castillo J, Calon A, Martínez-Ciarpaglini C, Peiró-Chova L, Huerta M, Zuñiga S, Rentero-Garrido P, Montón-Bueno J, Roda D, Roselló S, Bellosillo B, Vivancos A, Nyamundana G, Montagut C, Sadanandam A, Cervantes A.. Ann Oncol. 2018 Oct;29 Suppl 8:viii717. doi: 10.1093/annonc/mdy424.025.. Increased immune cell infiltration in patient-derived tumor explants treated with Traniplatin: an original Pt(iv) pro-drug based on Cisplatin and Tranilast.. Lo Re D, Montagner D, Tolan D, Di Sanza C, Iglesias M, Calon A*, Giralt ...

wheat flour, margarine (rapeseed oil, palm oil, water, salt, emulsifier: mono-and diglycerides of fatty acids, acidity regulator: citric acid, flavouring, vitamins A and D3), matured Cheddar cheese (milk)(22%), egg, yeast, dried milk, salt, sugar, potato starch, spices, yeast extract, emulsifier: mono-and diglycerides of fatty acids, colour: paprika extract ...

Background: Bacterial vaginosis (BV) is the most common vaginal disorder of reproductive-age women. Yet the cause of BV has not been established. To uncover key determinants of BV, we employed a multi-omic, systems-biology approach, including both deep 16S rRNA gene-based sequencing and metabolomics of lavage samples from 36 women. These women varied demographically, behaviorally, and in terms of health status and symptoms. Principal Findings: 16S rRNA gene-based community composition profiles reflected Nugent scores, but not Amsel criteria. In contrast, metabolomic profiles were markedly more concordant with Amsel criteria. Metabolomic profiles revealed two distinct symptomatic BV types (SBVI and SBVII) with similar characteristics that indicated disruption of epithelial integrity, but each type was correlated to the presence of different microbial taxa and metabolites, as well as to different host behaviors. The characteristic odor associated with BV was linked to increases in putrescine and ...

Femisan Cream is indicated in the treatment of the bacterial vaginosis originated by Gardnerella vaginalis, anaerobic Mobiluncus spp and other bacteria like Bacteroides fragilis, as well as in mixed vaginitis and vaginal candidiasis. Available now at Medicines Mexico online Mexican pharmacy! Mexico pharmacy drugs

APG IV Classification: Domain: Eukaryota • (unranked): Archaeplastida • Regnum: Plantae • Cladus: angiosperms • Cladus: monocots • Cladus: commelinids • Ordo: Zingiberales • Familia: Zingiberaceae • Genus: Elettariopsis • Species: Elettariopsis curtisii Baker (1892) ...

APHOTOFLORA - Photographic Stock Image Library Page for Agrostis curtisii - Bristle Bent (Grass Images). A-P-H-O-T-O - Furthering environmental awareness and education through the medium of photography.

In this datasheet, the authors have used a melanoma mouse model to study tumour-driven perturbations during haematopoiesis. First, they harvested cells from the spleen of tumour-burdened mice and the bone marrow of tumour-burdened and healthy mice. From these cells, they performed enrichment of negative lineage (Lin-) cells by magnetic enrichment using the BD IMag™ Haematopoietic Progenitor Cell Enrichment Set, supplemented with biotinylated antibodies for CD2, CD5 and CD19. Enriched Lin- cells from tumour-burdened bone marrows and spleens, and healthy bone marrows were individually stained with unique DNA-barcoded antibodies from the BD Mouse Immune Single-Cell Multiplexing Kit and then stained with a 6-color antibody fluorochrome panel and a 36-plex BD® AbSeq Panel. Four different populations from each tissue were sorted simultaneously using the BD FACSMelody™ Cell Sorter with 4-way sorting capability (12 tubes total). Each one of the four populations from each tissue was pooled into a ...

Community-acquired pneumonia (CAP) is a heterogeneous disease causing great morbidity, mortality and health care burden globally. Typing methods for discriminating different clinical conditions of the same disease are essential to a better management of CAP. Traditional typing systems based separately on clinical manifestations (such as PSI and CURB-65), pathogens(bacterial types, virulence, drug resistance, etc) or host immune state (immunocompetent, immunocompromised or immunodeficiency). Thus, they are barely able to represent the real disease status nor to precisely predict the mortality.. As the development of multi-omic technologies, the relatedness of different phenotypes at a molecular level have revolutionized our ability to differentiate among patients. Our study is aimed at establishing a novel molecular typing method of CAP. Multi-omic (including genomics, transcriptomes, and metabolisms) data obtained from enrolled CAP patients and isolated pathogens would be integrated analyzed and ...

THE UNMET NEED IN PRECISION MEDICINE. Pharma has invested heavily in genomics and big data to understand each patients genome to deliver targeted therapeutics, yet success rates for targeted therapies are low and uptake in clinical practice is patchy. Theres a growing realization that just genomics is not enough to achieve the promise of personalized therapeutics. A clear unmet need has emerged for a multi-omic approach that may offer a much greater chance of success. However, few comprehensive, multi-omic datasets exist and such data is difficult to access quickly as it is both costly and time consuming to initiate prospective data collection - especially in cancer. ...

https://doi.org/10.18632/oncotarget.14018 Weiqin Jiang, Yongfeng Ding, Yifei Shen, Longjiang Fan, Linfu Zhou, Zhi Li, Yi Zheng, Peng Zhao, Lulu Liu, Zhou Tong, Weijia Fang, Weilin Wang

|p style=width: 100%; height: 62px;>Dr. Christopher Stewart completed his Ph.D. at Northumbria University Newcastle upon Tyne in 2014 and later moved to the United States for his postdoctoral training. He is currently a Postdoctoral Researcher at Baylor College of Medicine (Houston, TX). His research interests are focused on the diversity and ecology of microbial communities and host responses in clinical samples and ex vivo model systems to determine the microbial-host interaction in health and disease.|br /> |br /> Dr. Stewarts work has used molecular, culture, proteomic and metabolomic techniques to explore a range of clinical samples, primarily from early life. He ran a molecular sequencing and mass spectrometry lab, developing and optimizing novel mass spectrometry methods for profiling the proteome and metabolome of clinical samples. He has extensive experience in bioinformatics and statistical analysis of complex multi-omic datasets. He has also developed a novel ex vivo co-culture system

|p style=width: 100%; height: 62px;>Dr. Christopher Stewart completed his Ph.D. at Northumbria University Newcastle upon Tyne in 2014 and later moved to the United States for his postdoctoral training. He is currently a Postdoctoral Researcher at Baylor College of Medicine (Houston, TX). His research interests are focused on the diversity and ecology of microbial communities and host responses in clinical samples and ex vivo model systems to determine the microbial-host interaction in health and disease.|br /> |br /> Dr. Stewarts work has used molecular, culture, proteomic and metabolomic techniques to explore a range of clinical samples, primarily from early life. He ran a molecular sequencing and mass spectrometry lab, developing and optimizing novel mass spectrometry methods for profiling the proteome and metabolome of clinical samples. He has extensive experience in bioinformatics and statistical analysis of complex multi-omic datasets. He has also developed a novel ex vivo co-culture

TY - JOUR. T1 - Implementing a comprehensive translational oncology platform. T2 - From molecular testing to actionability. AU - Mitri, Zahi I.. AU - Parmar, Swapnil. AU - Johnson, Brett. AU - Kolodzie, Annette. AU - Keck, Jamie M.. AU - Morris, Max. AU - Guimaraes, Alexander R.. AU - Beckett, Brooke R.. AU - Borate, Uma. AU - Lopez, Charles D.. AU - Kemmer, Kathleen A.. AU - Alumkal, Joshi J.. AU - Beer, Tomasz M.. AU - Corless, Christopher L.. AU - Mills, Gordon B.. AU - Gray, Joe W.. AU - Bergan, Raymond C.. N1 - Publisher Copyright: © 2018 The Author(s).. PY - 2018/12/14. Y1 - 2018/12/14. N2 - Background: In order to establish the workflows required to implement a real-time process involving multi-omic analysis of patient samples to support precision-guided therapeutic intervention, a tissue acquisition and analysis trial was implemented. This report describes our findings to date, including the frequency with which mutational testing led to precision-guided therapy and outcome for those ...

The antibiotics kill normal vaginal bacteria, which keep yeast in check. Yeast infections can be diagnosed during a medical exam.

Background: International recommendations in favor of screening for vaginal infection in pregnancy are based on heterogeneous criteria. In most developed countries, the diagnosis of bacterial vaginosis is only recommended for women with high-risk of preterm birth. The Nugent score is currently used, but molecular quantification tools have recently been reported with a high sensitivity and specificity. Their value for reducing preterm birth rates and related complications remains unexplored. This trial was designed to assess the cost-effectiveness of a systematic screen-and-treat program based on a point-of-care technique for rapid molecular diagnosis, immediately followed by an appropriate antibiotic treatment, to detect the presence of abnormal vaginal flora (specifically, Atopobium vaginae and Gardnerella vaginalis) before 20 weeks of gestation in pregnant women in France. We hypothesized that this program would translate into significant reductions in both the rate of preterm births and the medical

Purpose. Cervicovaginal Atopobium vaginae and Gardnerella vaginalis are strongly associated with bacterial vaginosis (BV) and are the main components of vaginal biofilms. The low efficacy of BV treatment with metronidazole may be due to the presence of such biofilms. Thus, the aim of this study was to compare the pretreatment cervicovaginal loads of A. vaginae and G. vaginalis for women who restored normal flora and those who persisted with BV after a full course of oral metronidazole. Methodology. In this cross-sectional study, 309 reproductive-aged women were recruited in a primary health care service in Botucatu, Brazil. Cervicovaginal samples were tested for genital tract infections, microscopic classification of local microbiota and molecular quantification of A. vaginae and G. vaginalis. Results. All the participants with concurrent cervicovaginal infections (n=64) were excluded. A total of 84 out of 245 (34.3 %) women had BV at enrolment and 43 (51.2 %) of them completed the treatment and

Clindamycin: Is a lincosamide antibiotic, which acts primarily against gram positive and a wide range of anaerobic bacteria. It is congener of lincomycin. Clindamycin inhibits bacterial protein synthesis by its action at the bacterial ribosome. The antibiotic binds preferentially to the 50S ribosomal subunit and effects the process of protein synthesis. Clindamycin has antimicrobial activity against Bacteroides spp., Gardenerellavaginalis, Mobiluncus spp., Mycoplasma hominis, Peptostreptococcus spp., Haemophilus spp., and Corynebacterium ...

Bacterial vaginosis. After the slide was gram stained the slide was scanned under a microscope using low power objective to locate clusters of epithelial cells. The flora in these areas was noted. The oil immersion lens (x1000) was switched and between 10 and 20 representative fields was examined to observe cell morphology and gram reaction. The BV score was calculated by Nugents method. Briefly, the average number of lactobacillary morphotypes per oil immersion field was quantitated. These organisms were usually filamentous, gram positive rods of varying length that often form chains, but occasionally, they stained gram negative. Also, the average number of Gardnerella spp. and anaerobic gram negative rods were quantitated. These may appear as small, gram variable pleomorphic coccobacilli. Finally, the amount of Mobiluncus morphotypes present was quantitated. They are often thin, wispy, eyelash-like faintly staining curved gram negative rods. Alternatively they may be much smaller ...

OBJECTIVE-Genital tract infection accounts for ~ 25-40% of all pre-term births. We sought to assess the relationship between preterm birth and selected vaginal bacterial taxa associated with preterm birth either directly or through their association with bacterial vaginosis (BV). STUDY DESIGN-Vaginal fluid for Gram stain was collected between 17 and 22 weeks gestation as part of a randomized trial of ultrasound-indicated cerclage for preterm birth prevention in women at high risk for recurrent spontaneous preterm birth. Bacterial DNA was extracted from the Gram stain slides and analyzed using quantitative PCR. RESULTS-Among the 499 participants, Mycoplasma was positively correlated with increased risk of preterm (RR = 1.83; 95% CI: 1.52,2.22) as was Mobiluncus (RR=1.36; 95% CI: 1.07, 1.73) and Atopobium (RR=1.44; 95% CI: 1.1, 1.87). However, there were strong interactions between race/ethnic group and the presence of these and other individual taxa on risk of preterm birth. By contrast, BVAB3 ...

OBJECTIVE-Genital tract infection accounts for ~ 25-40% of all pre-term births. We sought to assess the relationship between preterm birth and selected vaginal bacterial taxa associated with preterm birth either directly or through their association with bacterial vaginosis (BV). STUDY DESIGN-Vaginal fluid for Gram stain was collected between 17 and 22 weeks gestation as part of a randomized trial of ultrasound-indicated cerclage for preterm birth prevention in women at high risk for recurrent spontaneous preterm birth. Bacterial DNA was extracted from the Gram stain slides and analyzed using quantitative PCR. RESULTS-Among the 499 participants, Mycoplasma was positively correlated with increased risk of preterm (RR = 1.83; 95% CI: 1.52,2.22) as was Mobiluncus (RR=1.36; 95% CI: 1.07, 1.73) and Atopobium (RR=1.44; 95% CI: 1.1, 1.87). However, there were strong interactions between race/ethnic group and the presence of these and other individual taxa on risk of preterm birth. By contrast, BVAB3 ...

Banks et al. (1) suggested that plaque formation by chlamydiae might depend on the strains growth rates, and consequently, for slowly growing strains such as biovar trachoma long-term maintenance of the cell monolayer in agar medium might be required for plaque formation. In the present study, two points were crucial to the success of plaque formation by biovar trachoma. (i) The liquid culture medium had to be layered onto a solid agarose medium and changed at 4- to 5-day intervals; this treatment may refresh the cells and support chlamydial growth until plaque formation occurs. (ii) The agarose concentration was intentionally reduced to 0.5%, although 1% is the usual concentration for plaque formation by lytic viruses. The reduced agarose concentration may serve to keep the cells in good condition. The quality of the agarose was also important because purified agar was unsuitable for long-term maintenance of the cells. It is also likely that the agarose overlay might create appropriate ...

A longstanding goal in the Keasling Lab centers on the characterization and development of microbial hosts beyond those traditionally associated with synthetic biology and metabolic engineering (E. coli and S. cerevisiae). This includes developing detailed multi-omic understanding of select microbes and improved biological tools to manipulate these hosts. Recently, we have become ever more interested studying the complex metabolism of Streptomyces species and developing tools to allow reliable and rapid metabolic engineering in this bacterial genus. Given the immense scope of secondary metabolites produced by these bacteria and other favorable properties unique to Streptomyces venezuelae, we are committed to developing this bacterium for routine biotechnology efforts. Upcoming reports will detail the generation of improved biological tools (i.e., fluorescent reporters, improved promoters, Cas9 genome editing systems, etc.) that facilitate efforts aimed toward producing non-native products. ...

We present the genome-wide chromatin accessibility profiles of 410 tumor samples spanning 23 cancer types from The Cancer Genome Atlas. We identify 562,709 transposase-accessible DNA elements that substantially extend the compendium of known cis-regulatory elements. Integration of ATAC-seq with TCGA multi-omic data identifies a large number of putative distal enhancers that distinguish molecular subtypes of cancers, uncovers specific driving transcription factors via protein-DNA footprints, and nominates long-range gene-regulatory interactions in cancer. These data reveal genetic risk loci of cancer predisposition as active DNA regulatory elements in cancer, identify gene-regulatory interactions underlying cancer immune evasion, and pinpoint noncoding mutations that drive enhancer activation and may impact patient survival. These results suggest a systematic approach to understand the noncoding genome in cancer to advance diagnosis and therapy.. ...

Genome-wide, imputed, sequence, and structural data are now available for exceedingly large sample sizes. The needs for data management, handling population structure and related samples, and performing associations have largely been met. However, the infrastructure to support analyses involving complexity beyond genome-wide association studies is not standardized or centralized. We provide the PLatform for the Analysis, Translation, and Organization of large-scale data (PLATO), a software tool equipped to handle multi-omic data for hundreds of thousands of samples to explore complexity using genetic interactions, environment-wide association studies and gene-environment interactions, phenome-wide association studies, as well as copy number and rare variant analyses ...

The ERA-Net for Research Programmes on Rare Diseases (E-RARE) aims at maximising the efficiency and impact of research on rare diseases on a European scale.. The aim of the call is to enable scientists in different countries to build an effective collaboration on a common interdisciplinary research project based on complementarities and sharing of expertise, with a clear transnational research approach. The research projects have to focus on hypothesis-driven use of multi-omic integrated approaches for discovery of disease causes and/or functional validation in the context of rare diseases.. Interested researchers are requested to contact the SNSF Administrative Offices to clarify eligibility issues before submitting their pre-proposal.. For further details, please refer to the programmes official website. The submission deadline for pre-proposals is 6 February 2018.. Call Website ...

Transnational research projects on hypothesis-driven use of multi-omic integrated approaches for discovery of disease causes and/or functional validation in the context of rare diseases ...

Speaker: Prof. Elhanan Borenstein,Tel Aviv University / University of Washington. Title: Systems biology and model-based multi-omic analysis of the human microbiome. ...

TY - JOUR. T1 - Propionibacterium acnes and acne vulgaris: new insights from the integration of population genetic, multi-omic, biochemical and host-microbe studies.. AU - McLaughlin, Joseph. AU - Watterson, Steven. AU - Layton, Alison M. AU - Bjourson, AJ. AU - Barnard, Emma. AU - Mc Dowell, Andrew. PY - 2019/5/13. Y1 - 2019/5/13. N2 - The anaerobic bacterium Propionibacterium acnes is believed to play an important role in the pathophysiology of the common skin disease acne vulgaris. Over the last 10 years our understanding of the taxonomic and intraspecies diversity of this bacterium has increased tremendously, and with it the realisation that particular strains are associated with skin health while others appear related to disease. This extensive review will cover our current knowledge regarding the association of P. acnes phylogroups, clonal complexes and sequence types with acne vulgaris based on multilocus sequence typing of isolates, and direct ribotyping of the P. acnes strain population ...

Bacterial vaginosis (BV) is a common vaginal bacterial imbalance associated with risk for HIV and poor gynecologic and obstetric outcomes. Male circumcision reduces BV-associated bacteria on the penis and decreases BV in female partners, but the link between penile microbiota and female partner BV is not well understood. We tested the hypothesis that having a female partner with BV increases BV-associated bacteria in uncircumcised men. We characterized penile microbiota composition and density (i.e., the quantity of bacteria per swab) by broad-coverage 16S rRNA gene-based sequencing and quantitative PCR (qPCR) in 165 uncircumcised men from Rakai, Uganda. Associations between penile community state types (CSTs) and female partners Nugent score were assessed. We found seven distinct penile CSTs of increasing density (CST1 to 7). CST1 to 3 and CST4 to 7 were the two major CST groups. CST4 to 7 had higher prevalence and abundance of BV-associated bacteria, such as Mobiluncus and Dialister, than CST1 to 3.

The fluctuating vaginal bacteria may make some women more vulnerable to infection.. Bacterial vaginosis is linked to transmission of HIV and other sexually transmitted diseases, so this is a potentially significant risk factor for acquiring sexually transmitted diseases, says Stephen Dewhurst, Ph.D., at the University of Rochester Medical Center in New York.. Vaginal bacteria also can affect pregnancy and fertility. The composition of vaginal microbiota and of a mans sperm could mean that a woman is fertile with one man and infertile with another.. We need to rethink the way we approach womens health and treatment and diagnosis, Dr. Ravel says.. Always talk with your health care provider to find out more information.. ...

Many of the organisms associated with BV (e.g., G. vaginalis and anaerobic organisms such as Mobiluncus and Prevotella spp.) are known to produce acetic acid as a metabolic by-product (32). Al-Mushrif et al. (1) have shown that the fatty acids produced by these organisms impair chemotaxis. They further suggest that this slowing of the immune response allows further proliferation of the infecting organisms. It is therefore logical to expect elevated vaginal acetic acid concentrations in patients with BV.. The results presented in this study provide further evidence that the vaginal acetic acid level can be a useful diagnostic for BV. This assertion is supported by the results obtained by tracking patients in the treatment and recovery phases of an oral metronidazole treatment regimen. In the vast majority of cases the level of acetic acid detected fell sharply during the treatment period, crossing from above to below the 900-ppm threshold.. In this study, BV status was tracked by using the two ...

Specific bacteria living in the human vagina may play a previously unrecognized role in the sexual transmission of HIV. Ragon Institute researchers, working with young, healthy, South African women, found that individuals with vaginas dominated by pro-inflammatory bacterial species were at a 4-fold higher risk of acquiring HIV than those with healthy vaginal bacteria. Meanwhile, viruses in the female genital tract showed no correlation with HIV risk. The study appears Jan. 10 in Immunity.

Theres evidence that having good vaginal bacteria can be passed down from mother to daughter. Not because youre born with certain genes but because if a baby girl is delivered vaginally, shes exposed to her mothers vaginal bacteria at birth. If the mom has good bacteria, it may precondition the baby to grow good bacteria later on (since vaginal bacteria doesnt tend to accumulate until puberty), Ravel said. ...

Berg, a biopharmaceutical company committed to a data-driven, biological research approach, and the Icahn School of Medicine at Mount Sinai have formed a five-year pharmaceutical and diagnostic R&D partnership to leverage the power of multi-omic biology and data analytics to obtain novel insights and potential therapeutics for cancer, central nervous system and endocrine disorders. Berg will leverage its Interrogative Biology platform in combination with Mount Sinais experience in big data, advanced analytics and biological network modeling.. Berg and Mount Sinai will adopt an innovative risk and return sharing approach, with downstream royalties significantly higher than the industry standard for academia-pharma partnerships. The partnership with Mount Sinai flips the traditional public-private partnership model and sets a new standard for pharmaceutical research and development, said Niven R. Narain, co-founder, president and chief technology officer of Berg. Innovation in the healthcare ...

Research group The research group of Prof. Qiang Pan-Hammarström works on the molecular mechanism of immunoglobulin gene diversification, genetics of primary immunodeficiency and B-cell lymphomagenesis. The research laboratory is located at campus Flemingsberg. For more information about the group, please see https://ki.se/en/bionut/research-group-qiang-pan-hammarstrom Duties The selected postdoctoral fellow candidate will work in our multidisciplinary team of researchers including clinicians, molecular biologists and bioinformaticians, and will be jointly supervised by Prof. Qiang Pan-Hammarström and Prof. Lennart Hammarström, two international leading experts in the primary immunodeficiency field. The main tasks for the successful candidate are: 1) Identification of new forms of primary immunodeficiency through multi-omic analysis, with focus on late B cell deficiencies. This will involve analysis, interpretation, and integration of high throughput data derived from sequencing of the whole exome

BioFilm Pharma and BIOASTER (www.bioaster.org), the French Institute for Technological Research in Infectious Diseases and Microbiology, announced today the signature of a research contract.. To address the challenge of antibiotic resistance, this collaboration will lead to the deciphering of the mode of action of a new class of drugs.. This first project aims at identifying the mode of action of non-antibiotic anti-biofilms molecules through an integrated multi-omic analysis.. Anti-biofilm candidates of the study have already shown very promising results thanks to the BioFilm Ring Test® technology patented by the BioFilm Technologies group. As of today, this key technology is the only reliable and standardized in vitro biofilm method focusing on bacterial adhesion. Find more information on BIOASTER Press Release here.. ...

Doctors help you with trusted information about Bacterial Vaginosis in Bacterial Vaginosis: Dr. Sparacino on is there a home remedy for bacterial vaginosis: Bacterial vaginosis is an overgrowth of normal vaginal bacteria. The only real way to fix it is with antibiotics. There is an over the counter product called rephresh which can help normalize the pH of the vagina. It is better at preventing BV after treatment than using it for actual treatment.

The Atlas of Florida Plants provides a source of information for the distribution of plants within the state and taxonomic information. The website also provides access to a database and images of herbarium specimens found at the University of South Florida and other herbaria.

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Constraint-based modeling uses mass balances, flux capacity, and reaction directionality constraints to predict fluxes through metabolism. Although transcriptional regulation and thermodynamic constraints have been integrated into constraint-based modeling, kinetic rate laws have not been extensively used. In this study, an in vivo kinetic parameter estimation problem was formulated and solved using multi-omic data sets for Escherichia coli. To narrow the confidence intervals for kinetic parameters, a series of kinetic model simplifications were made, resulting in fewer kinetic parameters than the full kinetic model. These new parameter values are able to account for flux and concentration data from 20 different experimental conditions used in our training dataset. Concentration estimates from the simplified kinetic model were within one standard deviation for 92.7% of the 790 experimental measurements in the training set. Gibbs free energy changes of reaction were calculated to identify reactions that

Mark Marten. Photo courtesy of Marten. To understand how the fungal cell walls respond to environmental stressors, Marten and his team studied what he describes as the cells software-rules that control how the cell behaves. When fungi experience stress, Martens team found an increase in the number of septa created. Like bulkheads in a ship, septa prevent catastrophic loss of cellular cytoplasm if theres a break in the cell wall. When you stress cells, they sense it and try to protect themselves, Marten explains. He adds that fungi try to repair damage to their cell walls so that they can resume normal growth and function. The study used a multi-omic methodology, which researchers say can be applied to studying how signaling networks in cells work in general. The methodology allowed researchers to get a more detailed understanding of how cells respond to stressors. They found that when cell walls experience stress, there is a coordinated response through various pathways. By combining ...

The exact role of the babys gut bacteria is unclear and it isnt known if these differences at birth will have any effect on later health. The researchers found the differences in gut bacteria between vaginally born and caesarean delivered babies largely evened out by 1 year old, but large follow-up studies are needed to determine if the early differences influence health outcomes. Experts from the Royal College of Obstetricians and Gynaecologists say that these findings should not deter women from having a caesarean birth.. Published in Nature today (18th Sept), this largest ever study of neonatal microbiomes also revealed that the microbiome of vaginally delivered newborns did not come from the mothers vaginal bacteria, but from the mothers gut. This calls into question the controversial practice of swabbing babies born via caesarean with mothers vaginal bacteria. Understanding how the birth process impacts on the babys microbiome will enable future research into bacterial ...

Melissa Herbst-Kralovetz of the University of Arizona Cancer Center and UA College of Medicine -- Phoenix has found a direct relationship between good bacteria and cervical health and bad bacteria and increased cancer risk.