Hi Methods, I am fractionating baculovirus-infected Sf9 and Sf21 insect cells, using differential centrifugation to isolate microsomal membranes. The problem is that the microsomes (50,000 x g) are very particulate, and remain clumpy even after homogenization with hand-held glass/Teflon Potter-Elvehjem tissue grinder. Even mechanical disruption of the final microsomal pellet using a small polytron leaves clumps of unresuspended membranes that are visible by eye. The insect cell microsomes are clumpy when isolated either in solutions containing high salt (0.6 M KCl, 250 mM sucrose, 3 mM MgCl2) or low salt (10 mM NaHCO3, 0.1 mM CaCl2). The two different preparative protocols are listed below. Following centrifugation, the microsomal pellets are typically resuspended in 250 mM sucrose buffered with MOPS or histidine. Why do the microsomes clump so much? Are there solutes to add (pyrophosphate, NaI, etc) that would allow easy and complete resuspension of insect cell microsomes? Thanks, Mike Autry ...
Biological activity of porcine calcitonin was most actively inactivated by the rat kidney homogenate than by other tissue homogenates. Among the various subcellular fractions of the rat kidney homogenate examined, microsome fraction was most active in the in vitro inactivation of porcine calcitonin. Inactivation of porcine calcitonin by the rat kidney microsome was dependent on pH and temperature. Inactivating activity of the rat kidney microsome was inhibited by 1 X 10(-3) M monoiodoacetate and 1 X10(-5) M p-chloromercuribenzoate. These results suggest that porcine calcitonin is probably inactivated by a SH-enzyme in the rat kidney microsomes. However, the participation of other enzymes cannot be ruled out, since the inactivating activity of the rat kidney microsome fraction is also inhibited by 1 X 10(-4) M diisopropylfuorophosphate. ...
p,Hyperproduced prostaglandin E,sub,2,/sub, by cyclooxygenase-2 and microsomal prostaglandin E synthase-1 evokes several pathophysiological responses such as inflammation and carcinogenesis. Our recent study demonstrated that ,i,Dioscorea japonica,/i, extract suppressed the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 and induced apoptosis in lung carcinoma A549 cells. In the present study, we investigated the effects of ,i,Dioscorea japonica,/i, on squamous cell carcinoma of mouse skin. ,i,Dioscorea japonica,/i, feeding and ,i,Dioscorea japonica,/i, extract topical application suppressed the expression of cyclooxygenase-2, microsomal prostaglandin E synthase-1, interleukin-1β and interleukin-6 and inhibited tumor formation, hyperplasia and inflammatory cell infiltration. Immunohistochemical analyses showed the immunoreactivities of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 in tumor keratinocytes and stronger immunoreactivities of cyclooxygenase-2 ...
Flavin-containing monooxygenase (FMO) was partially purified from rat brain microsomes through two successive chromatographies on columns of DEAE Sepharose and 2,5-ADP Sepharose. The specific activity, benzydamine N-oxidation of partially purified brain FMO, was 122-fold higher than that of micros …
hypothetical protein, gbf1, Anapl_04335, AS27_01565, AS28_01193, C79137, C9orf15, CB1_000576027, D623_10025025, gamma-interferon-activated transcriptional element-binding factor 1, GATE-binding factor 1, GBF-1, H920_19196, I79_014770, M91_12705, M959_12051, MDA_GLEAN10019304, membrane-associated prostaglandin E synthase 2, membrane-associated prostaglandin E synthase-2, microsomal prostaglandin E synthase 2, microsomal prostaglandin E synthase-2, Mpges2, MPGES-2, mPGE synthase-2, N300_09271, N301_12743, N302_13172, N303_06092, N305_09010, N306_14810, N307_02442, N308_10508, N311_11677, N312_00533, N320_07005, N321_11509, N322_00413, N324_09565, N325_00199, N326_00389, N327_11175, N328_09700, N329_10988, N330_00570, N331_03110, N332_07603, N333_00239, N334_00603, N335_09322, N336_03658, N339_06101, N340_07113, N341_11017, PAL_GLEAN10012478, PANDA_003194, pges2, prostaglandin E receptor 2, prostaglandin E synthase 2-like protein, prostaglandin-H(2) E-isomerase, TREES_T100004307, UY3_04702, ...
TY - JOUR. T1 - In vitro biosynthesis of sialosylgalactosylceramide (G7) by mouse brain microsomes. AU - Yu, R. K.. AU - Lee, S. H.. N1 - Copyright: Copyright 2004 Elsevier B.V., All rights reserved.. PY - 1976. Y1 - 1976. N2 - A sialyltransferase activity which catalyzes the synthesis of sialosylgalactosylceramide (G7) from added galactocerebroside and CMP N acetylneuraminic acid has been demonstrated in mouse brain microsomes. The enzyme reactions shows a pH optimum of 6.3 and requires detergents. Both Mn2+ and Ca2+ inhibited the reaction, whereas Mg2+ had no effect. The apparent Km for galactocerebroside leading to G7 was estimated to be 8.7 x 10-4 M. The same microsomal preparation also synthesized hematoside when ceramide lactoside was the glycolipid acceptor. The apparent Km for ceramide lactoside was about 1/10th that for galactocerebroside. When the preparations were partially inactivated by heat the synthesis of G7 and of hematoside was reduced at approximately the same rate. Liver ...
Microsomal prostaglandin E synthase-1 (mPGES-1) or Prostaglandin E synthase is an enzyme that in humans is encoded by the PTGES gene. The protein encoded by this gene is a glutathione-dependent prostaglandin E synthase. The expression of this gene has been shown to be induced by proinflammatory cytokine interleukin 1 beta (IL1B). Its expression can also be induced by tumor suppressor protein TP53, and may be involved in TP53-induced apoptosis. Knockout studies in mice suggest that this gene may contribute to the pathogenesis of collagen-induced arthritis and mediate acute pain during inflammatory responses. Prostaglandin E synthase GRCh38: Ensembl release 89: ENSG00000148344 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000050737 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Jakobsson PJ, Thorén S, Morgenstern R, et al. (1989). Identification of human prostaglandin E synthase: a microsomal glutathione-dependent, inducible enzyme, constituting a potential ...
TY - JOUR. T1 - Cell-free synthesis of enzymically active tissue-type plasminogen activator. T2 - Protein folding determines the extent of N-linked glycosylation. AU - Bulleid, N. J.. AU - Bassel-Duby, R. S.. AU - Freedman, R. B.. AU - Sambrook, J. F.. AU - Gething, M. J H. PY - 1992. Y1 - 1992. N2 - Tissue-type plasminogen activator (t-PA) is synthesized in mammalian cells as a mixture of two forms that differ in their extent of N-linked glycosylation. We have investigated the mechanism underlying this variation in glycosylation, using a cell-free system that consists of a rabbit reticulocyte lysate optimized for the formation of disulphide bonds and supplemented with dog pancreas microsomal membranes. Molecules of human t-PA synthesized in vitro are enzymically active and responsive to natural activators and inhibitors, and are glycosylated in a pattern identical with that of the protein produced in vivo. This demonstrates that t-PA synthesized in vitro folds into the same conformation as the ...
BioAssay record AID 204026 submitted by ChEMBL: Inhibitory potency of compound against neutral (N-) SMase activity in bovine brain microsomes(Ms) at concentration of 400 uM.
BioAssay record AID 147531 submitted by ChEMBL: Inhibitory activity against schyphostatin of neutral sphingomyelinase (N-SMase) from bovine brain microsome.
The plant endomembrane system plays vital roles for synthesis, modification and secretion of proteins and lipids. From the classic view, only mRNAs encoding secreted proteins could be targeted to the endoplasmic reticulum (ER) for translation via a co-translational translocation manner, however, recently this model has been challenged by accumulative evidence that lots of cytosolic mRNAs could also associate with ER, and that some categories of small RNAs are enriched on ER. These results suggested unrevealed functions of ER beyond our current knowledge. The large scale identification of RNAs and proteins on microsome is crucial to demonstrating the ER function and the studies will be boosted by next generation sequencing technology. This protocol provides a technical workflow to isolate the cytosol, microsome, free polysome (FP) and membrane bound polysome (MBP) from plant tissue. The isolated fractions are suitable for genome wide profiling of mRNAs, small RNAs and proteins.
Inflammation comprises the reaction of the body to injury, in which a series of changes of the terminal vascular bed, blood, and connective tissue tends to eliminate the injurious agent and to repair the damaged tissue. It is a complex process, which involves the release of diverse regulatory mediators. The current anti-inflammatory agents are challenged by multiple side effects and thus, new effective therapies are highly needed. The aim of this review is to summarize the described microsomal prostaglandin E synthase-1 (mPGES-1) inhibitors or transcriptional suppressors from medicinal plants, which could be an ideal approach in the management of inflammatory disorders, but need further clinical trials in order to be ultimately validated ...
N-(3,4-dichlorobiphenyl-4-yl)-4-hydroxy-2H-1,2-benzothiazine-3-carboxamide 1,1-dioxide: a microsomal prostaglandin E synthase-1 inhibitor; structure in first source
There are various subcellular fractions and they include homogenate, s9, microsomes and cytosol. These products are valuable tools in drug discovery for studying the metabolism of your compounds. Human Subcellular Products InVitroCYP™ 150-Donor Human Liver Microsomes InVitroCYP™ H-Class Human Liver Microsomes InVitroCYP™ M-Class Human Liver Microsomes Human Microsomes - Other Tissues Human Liver S9
Rates of lung microsomal dimethylaniline (DMA) demethylation and N-oxidation as well as cytochrome P-450 concentrations from 20- and 28-day pregnant rabbits were observed to be as much as 2 times the values measured in lung microsomes from adult female nonpregnant rabbits. These increases were not seen in lung microsomes from 10-day pregnant rabbits, and only small increases were observed in lung microsomes from 15-day pregnant rabbits. In contrast, no differences were seen in hepatic microsomal DMA demethylase or N-oxidase activities at any stage of pregnancy. An attempt was made to correlate high plasma concentrations of steroids during pregnancy with the elevated enzyme activities observed in lung microsomes. Adult nonpregnant rabbits were treated with a variety of steroids, the concentrations of which have been shown to increase in plasma during late pregnancy. No effect on lung or liver microsomal DMA metabolism was observed when rabbits were treated with testosterone propionate. However, ...
A solid phase radioimmunoassay for adrenal antibodies is described. In the assay plastic tubes coated with adrenal microsomes (100 micrograms/ml) were incubated with human sera diluted from 1:50 to 1:5000 and the retained antibodies detected by subse
Gentaur molecular products has all kinds of products like :search , SeraLab \ Beagle Liver Microsomes \ MIC-114 for more molecular products just contact us
Caltag Medsystems is a UK distributor of diagnostic and research reagents for cell biology, flow cytometry, immunology, histology and neuroscience. We offer over 200,000 high quality products at competitive prices
Looking for online definition of Antimicrosomal antibody test in the Medical Dictionary? Antimicrosomal antibody test explanation free. What is Antimicrosomal antibody test? Meaning of Antimicrosomal antibody test medical term. What does Antimicrosomal antibody test mean?
Caco-2 cell lysate, and intestinal and liver microsomes derived from female humans and rats were used to compare and contrast the metabolism and disposition of raloxifene. In Caco-2 cell lysate, raloxifene 6-β-glucuronide (M1) was the main metabolite, although raloxifene 4′-β-glucuronide (M2) was formed in comparable abundance (58% versus 42%). In rat liver and intestinal microsomes, M1 represented about 76 to 86% of glucuronidated metabolites. In contrast, raloxifene 4′-β-glucuronide (M2) was the predominant metabolite in expressed UGT1A10 (96%) and human intestinal (92%) microsomes. Intrinsic clearance for M2 (CLint, M2) in human intestinal microsomes was 33- to 72-fold higher than in rat microsomes, whereas intrinsic clearance for M1 (CLint, M1) was 3- to 4-fold lower. Taken together, total intrinsic clearance (CLint, M1 + CLint, M2) in human intestinal microsomes was 3- to 6-fold higher than that in rat intestinal microsomes, but was similar in liver microsomes. In addition, intrinsic
Abstract: Fatty acid composition and lipid peroxidation in rat brain microsomes were studied under conditions of alloxan diabetes. The data obtained indicate significant differences in the content of unsaturated fatty acids and also a slight activation of lipid peroxidation in the pathological state studied ...
In cell biology, microsomes are heterogenous vesicle-like artifacts (~20-200 nm diameter) re-formed from pieces of the endoplasmic reticulum (ER) when eukaryotic cells are broken-up in the laboratory; microsomes are not present in healthy, living cells.[1] Microsomes can be concentrated and separated from other cellular debris by differential centrifugation. Unbroken cells, nuclei, and mitochondria sediment out at 10,000 g, whereas soluble enzymes and fragmented ER, which contains cytochrome P450 (CYP), remain in solution (g is the Earths gravitational acceleration). At 100,000 g, achieved by faster centrifuge rotation, ER sediments out of solution as a pellet but the soluble enzymes remain in the supernatant. In this way, cytochrome P450 in microsomes is concentrated and isolated. Microsomes have a reddish-brown color, due to the presence of the heme. Because of the need for a multi-part protein-system, microsomes are necessary to analyze the metabolic activity of CYPs. These CYPs are highly ...
Hepatic microsomal cytochrome P450s, which are involved in the metabolism of drugs, hormones, prostaglandins and fatty acids, change when animals develop diabetes. We studied changes in cytochrome P450 isozymes in both hepatic and renal microsomes of rats with diabetes caused by streptozocin, and co …
Microsomal prostaglandin E synthase-1 (mPGES-1) constitutes a drug target for inflammation, fever and pain. mPGES-1 catalyzes the biosynthesis of prostaglandin (PG) E2 from cyclooxygenase (Cox) -derived PGH2, which in turn is derived from arachidonic acid. mPGES-1 is mainly associated with inflammation and it is known to be up regulated by various pro-inflammatory cytokines like IL-1 beta and TNF-alpha. Mice devoid of mPGES-1 activity display resistance to development of experimental arthritis, fever, pain, symptoms following stroke, atherosclerosis and breathing anomalies induced by hypoxia. Conversely, the enzyme seems to have a protective role in wound healing and remodeling following myocardial infarction. Inhibitors of mPGES-1 have been developed by several groups. However, their characterization in animal models of inflammation, or other models previously used to study mPGES-1 knock-out mice, remains limited. One reason is the fact that a majority of the potent inhibitors of human mPGES-1 ...
hepatic microsomes female wistar hepatic microsomes female wistar rat | order hepatic microsomes female wistar hepatic microsomes female wistar rat | How to use: hepati
The effects of several lactones were studied in a microsomal fraction of dog myocardium thought to be sarcoplasmic reticulum. The lactones increased the steady-state accumulation and turnover of calcium only in the presence of ATP, and augmented the calcium-stimulated ATPase activity. When the effective concentrations of the lactones were exceeded, there were no further alterations in calcium accumulation or turnover. A correlation between the capacity of these lactones to increase calcium accumulation and turnover and their relative cardiotonic activity, as reported in the literature, was noted. The potency of the lactones in relation to calcium metabolism in the microsomes is influenced by ring saturation, position of the double bond, and presence of a steroid ring system to the lactone moiety.. ...
Ribosomes synthesizing nascent secretory proteins are targeted to the membrane by the signal recognition particle (SRP), a small ribonucleoprotein that binds to the signal peptide as it emerges from the ribosome. SRP arrests further elongation, causing ribosomes to stack behind the arrested ribosome. Upon interaction of SRP with its receptor on the ER membrane, the translation arrest is released and the ribosome becomes bound to the ER membrane. We have examined the distribution of unattached and membrane-bound ribosomes during the translation of mRNAs encoding two secretory proteins, bovine preprolactin and rat preproinsulin I. We find that the enhancement of ribosome stacking that occurs when SRP arrests translation of these proteins is relaxed in the presence of microsomal membranes. We also demonstrate that two previously described populations of membrane-associated ribosomes, distinguished by their sensitivity to high salt or EDTA extraction, correspond to ribosomes that have synthesized ...
Human liver microsomes are subcellular fractions that contain drug-metabolizing enzymes including CYP enzymes, flavin monooxygenases, and UDP glucuronyl transferases. InVitroCYP M-class microsomes are designed to exhibit moderate CYP activity, ideal for clearance and metabolite identification studies. BioreclamationIVT extensive characterization and tissue profiling process guarantees that each lot of InVitroCYP M-class microsomes will provide consistent and reproducible results for your metabolism and clearance studies.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
The biotransformation and excretion of methylcyclopentadienyl manganese tricarbonyl (MMT) has been studied in vivo in the rat, and in vitro by using rat liver and lung microsomes. Orally administered 3H-MMT is efficiently absorbed, metabolized, and excreted in the urine as two major metabolites, (CO)3MnC5H4CO2H and (CO)3MnC5H4CH2OH, which account for 67% and 14% of the urinary tritium, respectively. There metabolites are also excreted in significant quantities in bile, but undergo reabsorption and excretion by the kidney since only a small fraction of the administered tritium appears in the feces. In vitro MMT was rapidly metabolized by a cytochrome P-450-dependent process inducible in liver but not in lung microsomes. In vivo induction by phenobarbital doubles the rate of biliary excretion of MMT metabolites and confers a remarkable protection against the toxic effect of MMT. ...
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
We offer discounted hepatic and extrahepatic microsomes, S9, and cytosol from a variety of representative preclinical species, including canine (dog), mouse, non-human primate (monkey), and rat.
Gentaur molecular products has all kinds of products like :search , SeraLab \ Bovine Microsomes \ MIC-202 for more molecular products just contact us
Landais, Elise, et al. HIV Envelope Glycoform Heterogeneity and Localized Diversity Govern the Initiation and Maturation of a V2 Apex Broadly Neutralizing Antibody Lineage. Immunity. 47.5 (2017): 990-1003.e9. ...
TY - JOUR. T1 - Squalene synthetase. Solubilization from yeast microsomes of a phospholipid requiring enzyme. AU - Agnew, William. AU - Popjak, G.. PY - 1978. Y1 - 1978. UR - http://www.scopus.com/inward/record.url?scp=0017821357&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0017821357&partnerID=8YFLogxK. M3 - Article. C2 - 350878. AN - SCOPUS:0017821357. VL - 253. SP - 4574. EP - 4583. JO - Journal of Biological Chemistry. JF - Journal of Biological Chemistry. SN - 0021-9258. IS - 13. ER - ...
Prostaglandin E synthase (PGES) including isoenzymes of membrane-associated PGES (mPGES)-1, mPGES-2, and cytosolic PGES (cPGES) is the recently identified terminal enzyme of the arachidonic acid cascade. PGES converts prostaglandin (PG)H2 to PGE2 downstream of cyclooxygenase (COX). We investigated the expression of PGES isoenzyme in articular chondrocytes from patients with osteoarthritis (OA). Chondrocytes were treated with various cytokines and the expression of PGES isoenzyme mRNA was analyzed by the reverse transcription-polymerase chain reaction and Northern blotting, whereas Western blotting was performed for protein expression. The subcellular localization of mPGES-1 was determined by immunofluorescent microscopy. Conversion of arachidonic acid or PGH2 to PGE2 was measured by enzyme-linked immunosorbent assay. Finally, the expression of mPGES-1 protein in OA articular cartilage was assessed by immunohistochemistry. Expression of mPGES-1 mRNA in chondrocytes was significantly induced by
Buy our Recombinant Human Microsomal Glutathione S-transferase 1 protein. Ab114792 is a full length protein produced in Wheat germ and has been validated in…
1. Guanosine 5′-[gamma-thio]triphosphate (GTP[S]), if added before GTP, blocks both Ca2+ efflux promoted by GTP and the effect of GTP on enhancement of inositol 1,4,5-triphosphate (IP3)-promoted Ca2+ release from preloaded microsomal vesicles. If, however, GTP[S] is added after GTP, it does not reverse the Ca2+ efflux promoted by GTP, nor does it inhibit IP3-promoted Ca2+ release. 2. The effect of GTP in enhancing IP3-promoted Ca2+ release is maintained after washing the microsomal vesicles free of added GTP. After this treatment, enhancement of IP3-promoted Ca2+ efflux can be observed in the absence of poly(ethylene glycol). 3. Electron microscopy shows that during GTP treatment of microsomal vesicles there is rapid production of very large vesicular structures, apparently produced by fusion of smaller vesicles. 4. Light-scattering changes are detectable during the fusion process. 5. Both Ca2+ efflux promoted by GTP and the enhancement of IP3-promoted Ca2+ release seen in the presence of GTP ...
Rodent skin (extrahepatic) subcellular fractions, including matching pooled IGS Sprague-Dawley rat microsomes and S9 from dermal tissue, characterized for in vitro ADME studies to test the biotransformation of transdermal xenobiotics.
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
ID: http://www.ncbi.nlm.nih.gov/gene/4259 Type: http://bio2vec.net/ontology/gene Label: MGST3 Synonyms: MGST3, GST-III, microsomal glutathione S-transferase 3, microsomal GST-3, microsomal GST-III, microsomal glutathione S-transferase III Alternative IDs: als API: GO SPARQL: GO ...
PTGES2 - PTGES2 (untagged)-Human prostaglandin E synthase 2 (PTGES2), transcript variant 1 available for purchase from OriGene - Your Gene Company.
Cheung S-Y, Werner M, Esposito L, Troisi F, Cantone V, Liening S, König S, Gerstmeier J, Koeberle A, Bilancia R, Rizza R, Rossi A, Roviezzo F, Temml V, Schuster D, Stuppner H, Schubert-Zsilavecz M, Werz O, Hanke T, Pace S (2018) Discovery of a benzenesulfonamide-based dual inhibitor of microsomal prostaglandin E2 synthase-1 and 5-lipoxygenase that favorably modulates lipid mediator biosynthesis in inflammation. Eur J Med Chem 156:815-830. ...
Complete information for PTGES3 gene (Protein Coding), Prostaglandin E Synthase 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Shao, these discrepancies could partially reflect the relative amounts of active cytochrome isozymes in microsome preparations, as suggested by the lower rate of synthesis of Augmentn observed in the preparations of Kumar et al. 5 10. 1) and in OCT scans (Fig.
MGST1 overexpression lysate, 0.1 mg. Transient overexpression lysate of microsomal glutathione S-transferase 1 (MGST1), transcript variant 1c
Ribophorins I and II, two transmembrane glycoproteins characteristic of the rough endoplasmic reticulum (ER) are thought to be part of the translocation apparatus for proteins made on membrane bound polysomes. To study the stoichiometry between ribophorins and membrane-bound ribosomes we have determined the RNA and ribophorin content in rat liver microsomes or in microsomal subfractions of different density (i.e., ribosome content). The specificity of antibodies against the ribophorins was demonstrated by Western blot analysis of rat liver rough microsomes separated by 2-dimensional gel electrophoresis. The ribophorin content of microsomal subfractions was determined by indirect immunoprecipitation and for ribophorin I by a radioimmune assay. In the latter assay a molar ratio of ribophorin I/ribosomes approaching one was calculated for total microsomes as well as in the gradient subfractions. We therefore suggest that ribophorins mediate the binding of ribosomes to endoplasmic reticulum ...
Polycyclic aromatic hydrocarbons (PAHs) are products of incomplete combustion that are commonly inhaled by workers in the dusty trades. Many PAHs are metabolized by cytochrome P-4501A1 (CYP1A1), which may facilitate excretion but may activate pulmonary carcinogens. PAHs also stimulate their own metabolism by inducing CYP1A1. Recent studies suggest that respirable coal dust exposure inhibits induct
TY - JOUR. T1 - Liver microsomes contain multiple forms of inositol 1,4,5-trisphosphate binding proteins. T2 - Identification by nitrocellulose blot overlay. AU - Ali, Nawab. AU - Agrawal, Devendra K.. PY - 1992. Y1 - 1992. N2 - A group of proteins binding to inositol 1,4,5-trisphosphate (IP3) has been identified in rat liver microsomes by a nitrocellulose blot-overlay technique. Proteins were resolved by SDS-PAGE, blotted on nitrocellulose and incubated with [32P]IP3 followed by autoradiography. Approximately eight IP3-binding polypeptides ranging Mr 23-50 kDA were present exclusively in microsomes; these were absent from plasma membrane and mitochondrial fractions. Binding of [32P]IP3 to these proteins was displaceable to a great extent by 5 μM unlabeled IP3 but not by 10 μM IP1, IP2, IP4, ATP, or GTPγS. These results suggest that liver microsomes contain multiple forms of IP3-binding proteins that can be detected by this new method.. AB - A group of proteins binding to inositol ...
Aryl hydrocarbon hydroxylase induction by polycyclic hydrocarbons is expressed as a simple autosomal dominant trait; in any mouse homozygous or heterozygous for the allele Ah, the monooxygenase activity is generally induced by aromatic hydrocarbons in many tissues regularly containing the inducible enzyme system. Induction of hydroxylase activity by 3-methylcholanthrene administration to C57BL/6N mice is associated with an approximately equal conversion of hepatic type b to type a P-450, as measured by n-octylamine binding to the cytochrome. This conversion of mouse hepatic P-450 from one form to the other may also occur in rat and hamster liver microsomes and in kidney microsomes of the mouse, rat, or hamster. However, there is not a precise correlation in liver or kidney microsomes from control, 3-methylcholanthrene-treated, or phenobarbital-treated mice, rats, or hamsters between the sum of type a plus type b P-450 and the total P-450 concentration, as measured by difference spectra of the ...
TY - JOUR. T1 - Purification and characterization of a unique isozyme of cytochrome P-450 from liver microsomes of ethanol-treated rabbits. AU - Koop, D. R.. AU - Morgan, E. T.. AU - Tarr, G. E.. AU - Coon, M. J.. N1 - Copyright: Copyright 2004 Elsevier B.V., All rights reserved.. PY - 1982. Y1 - 1982. N2 - A new isozyme of cytochrome P-450 has been purified to electrophoretic homogeneity from hepatic microsomes of rabbits treated chronically with ethanol. Several criteria indicate that the ethanol-inducible cytochrome, which has a minimal molecular weight of 51,000 and is designated form 3a on the basis of its relative electrophoretic mobility, is distinct from the known isozymes of P-450. As judged spectrally, the new isozyme is high spin the oxidized state, as is form 4, but differs in that the spin state is unperturbed by nonionic detergents. The absolute spectrum of the ferrous carbonyl complex of form 3a is red shifted as compared to that of forms 2, 3b, 3c, 4, and 6 and exhibits a maximum ...
Find distinction microsomal prostaglandin. Mississsippi Stem Cell Therapy Center promotes stem cell therapy and regenerative medicine.
Liver slices from normal rats and those suffering from inflammation for 24-48 h were incubated with L-[-14C]leucine or D-[-14C]glucosamine. Immunological techinques coupled with radioautography indicated that the microsome fraction prepared from slices contained the subcellular site of synthesis of the polypeptide chain of serum albumin, and the polypeptide and carbohydrate chains of alpha-1-acid glycoprotein; both proteins were also present in the medium in labelled forms. The contents of albumin and alpha-1-acid glycoprotein in the medium and in extracts of liver from experiments with liver slices from control rats and 8-72 h experimental rats were determined using the quantitative precititin technique. There was a net increase in synthesis of both proteins when slices from control and experimental animals were used, the increase showing up in medium protein. However, slices from livers from 8-72 h experimental rats had a greater capacity for synthesis of alpha-1-acid glycoprotein and lower capacity
The aim of the study was to identify the cytochrome P450s (CYPs) that catalyze the biotransformation of clomipramine in vitro. A high-performance liquid chromatography method was developed to assay N-desmethylclomipramine, 8-hydroxyclomipramine, 2-hydroxyclomipramine, 8-hydroxydesmethhylclomipramine, didesmethylclomipramine and 2-hydroxydesmethylclomipramine formed by microsomes prepared from human liver and yeast expressing human CYP1A1, 1A2, 2C8, 2C9, 2C18, 2C19, 2D6 and 3A4. There was a statistically significant correlation between the formation rate of desmethylclomipramine and the immunoquantified concentration of CYP3A4 in 12 human liver microsome preparations (rs = 0.664, P = .028). Ketoconazole was a very potent inhibitor of desmethylclomipramine formation (Ki = 0.054 microM) and microsomes from yeast expressing CYP3A4 were also active in forming the metabolite (formation rate: 25.6 nmol/nmol of CYP per hr). Thus, the results are consistent with the assumption that the N-demethylation of ...
Members of the NSCC2 family have been sequenced from various yeast, fungal and animals species including Saccharomyces cerevisiae, Drosophila melanogaster and Homo sapiens. These proteins are the Sec62 proteins, believed to be associated with the Sec61 and Sec63 constituents of the general protein secretary systems of yeast microsomes. They are also the non-selective cation (NS) channels of the mammalian cytoplasmic membrane. The yeast Sec62 protein has been shown to be essential for cell growth. The mammalian NS channel proteins has been implicated in platelet derived growth factor(PGDF) dependent single channel current in fibroblasts. These channels are essentially closed in serum deprived tissue-culture cells and are specifically opened by exposure to PDGF. These channels are reported to exhibit equal selectivity for Na+, K+ and Cs+ with low permeability to Ca2+, and no permeability to anions ...
Rat liver microsomes attached to nanoparticles were used for LC-MS studies of CYP3A and 2E1 enzymes in metabolism of N-nitroso compounds. Using these biocolloids, turnover rates were measured within 2 min. Inhibitor IC50 values for ketoconazole (KET) and 4-methylpyrazole (4-MEP) were estimated.
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed ...
We provide biomedical researchers with the best tools available for the analysis of Oxidative Stress and Chronic Inflammation - two of the most important risk factors that play key roles in the development of a wide range of human illness, including cancer, cardiovascular disease, diabetes and neurodegeneration.. ...