We demonstrate ordered orientation of the hydration water at the surface of phospholipid bilayers by use of coherent anti-Stokes Raman scattering (CARS) microscopy, a highly sensitive vibrational imaging method recently developed. We investigated negatively charged POPS (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine) and neutral POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) multilamellar onions dispersed in deuterated dodecane. The imaging contrast based on the CARS signal from the H2O stretching vibration shows a clear dependence on the excitation field polarization. Our results provide direct experimental evidence that water molecules close to the phospholipid bilayer surface are ordered with the symmetry axis along the direction normal to the bilayer. Moreover, the amount of ordered water molecules depends on the lipid polar group. The spectral profile for the interlamellar water shows that the water molecules bound to the bilayer surface are less hydrogen-bonded and exhibit a ...

We report a mechanistic analysis of photodamage in coherent anti-Stokes Raman scattering (CARS) microscopy. Photodamage to the myelin sheath in spinal tissues is induced by using the point scan mode and is featured by myelin splitting and shockwaves with broadband emission. Our measurement of photodamage rate versus the excitation power reveals that both linear and nonlinear mechanisms are involved. Moreover, we show that vibrational absorption induced by coherent Raman processes significantly contributes to the nonlinear damage at high peak powers. For CARS imaging of cultured cells, the photodamage is characterized by plasma membrane blebbing and is dominated by a second order mechanism. Our study suggests that for dense samples such as the myelin sheath, CARS imaging induced photodamage can be minimized by using laser beams with relatively long near IR wavelengths and a repetition rate of a few MHz. For less dense samples such as cultured cells, laser pulses of higher repetition rates are ...

Nanoparticulate drug delivery is known to greatly improve the efficacy of pharmaceuticals and has found a wide range of applications with different administration methods including oral, intravenous, transcutaneous and ocular routes. However, the mechanisms by which these nanoparticles travel through, interact with, and modify tissues and how this relates to the improved drug performance are still unclear. These are critical questions that need to be answered to optimise the properties of future pharmaceuticals, dose rates and frequencies, and reduce potential side effects. Our ability to answer these questions is greatly hindered by the fact that there is currently no imaging modality available to directly visualise such small particles and the structure and function of the surrounding tissue, without the aid of contrast agents or highly invasive methods. Current imaging modalities derive image contrast of the nanoparticles and/or the surrounding tissues by means of external labels. We present ...

Coherent Anti-Stokes Raman Scattering (CARS)-microscopy has in recent years developed as a promising microscopical technique for label-free microscopy of living cells. We propose a new concept, spectral focusing, for highly efficient coherent anti-Stokes Raman scattering (CARS) microscopy. It allows optimal use of the excitation energy of femtosecond laser pulses in terms of generated CARS signal against a low background. This is accomplished by introducing a linear chirp in the excitation pulses. The temporal delaying of the excitation pulses can be used to record vibrational spectra of a sample. Despite the inherently broad spectral width of the excitation pulses, the technique enables resolution of spectral features 60 times narrower than the bandwidth of the probe light. First applications of this technique are exemplified with CARS of micron sized crystallites of sodium nitroprusside, a commonly used hypotensive agent ...

TY - JOUR. T1 - Stimulated Raman scattering microscopy with long wavelengths for improved imaging depth. AU - Moester, Miriam J.B.. AU - Zada, Liron. AU - Fokker, Bart. AU - Ariese, Freek. AU - de Boer, Johannes F.. PY - 2019/9. Y1 - 2019/9. N2 - Stimulated Raman scattering (SRS) imaging is a fast, label-free, and sensitive technique to map the distribution of a vibrational species in a microscopy setting. It has great potential for applications in many fields, such as lipid imaging in biomedicine. However, depth penetration of the light into the sample is an issue with any light-based technique, especially with multiphoton techniques such as SRS. Using longer wavelengths allows deeper penetration into densely scattering materials, but applying wavelengths above 1,500 nm is challenging technically. We have built a flexible SRS microscope system capable of imaging with a combination of 1,064 nm and wavelengths over 1,500 nm, using the idler output of an optical parametric oscillator (OPO). For ...

The clinical development of therapeutic peptides has been restricted to peptides for non-CNS diseases and parenteral dosage forms due to the poor permeation of peptides across the gastrointestinal mucosa and the blood-brain barrier. Quaternary ammonium palmitoyl glycol chitosan (GCPQ) nanoparticles facilitate the brain delivery of orally administered peptides such as leucine(5)-enkephalin, and here we examine the mechanism of GCPQ facilitated oral peptide absorption and brain delivery. By analyzing the oral biodistribution of radiolabeled GCPQ nanoparticles, the oral biodistribution of the model peptide leucine(5)-enkephalin and coherent anti-Stokes Raman scattering microscopy tissue images after an oral dose of deuterated GCPQ nanoparticles, we have established a number of facts. Although 85-90% of orally administered GCPQ nanoparticles are not absorbed from the gastrointestinal tract, a peak level of 2-3% of the oral GCPQ dose is detected in the blood 30 min after dosing, and these GCPQ ...

C. L. Evans, Potma, E. O., Puorishaag, M., Côté, D. C., Lin, C. P., et X Xie, S., « Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy. », Proc Natl Acad Sci U S A, vol. 102, nᵒ 46, p. 16807-12, 2005. ...

1. Wang P, Liu B, Zhang DL, Belew, MY, Tissenbaum HA, Cheng JX*. Imaging Lipid Metabolism in Live Caenorhabditis elegans Using Fingerprint Vibrations. Angewandte Chemie International Edition. 2014; 53:11787-11792 (In press; Impact factor: 13.7). 2. Wang P, Li JJ, Wang P, Hu CR, Zhang DL, Sturek, M, Cheng JX*. Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy. Angewandte Chemie International Edition. 2013; 52:13042-6 (Impact factor: 13.7). 3. Zhang D#, Wang P#, Slipchenko MN, Ben-Amotz D, Weiner AM, Cheng JX*. Quantitative vibrational imaging by hyperspectral stimulated Raman scattering microscopy and multivariate curve resolution analysis. Analytical chemistry. 2013;85(1):98-106. # Equal contribution first author. (Impact factor: 5.8). 4. Liao CS#, Slipchenko MN#, Wang P#, Hu CR, Li JJ, Oglesbee RA, Cheng JX*. Microsecond Scale Vibrational Spectroscopic Imaging by Multiplex Stimulated Raman Scattering Microscopy. Light: ...

Early diagnosis of tuberculosis (TB) and multidrug resistant tuberculosis (MDR TB) is important for the elimination of TB. We evaluated the microscopic observation drug susceptibility (MODS) assay as a direct rapid drug susceptibility testing (DST) method for MDR-TB screening in sputum samples All adult TB suspects, who were newly presenting to Pham Ngoc Thach Hospital from August to November 2008 were enrolled into the study. Processed sputum samples were used for DST by MODS (DST-MODS) (Rifampicin (RIF) 1 μg/ml and Isoniazid (INH) 0.4 μg/ml), MGIT culture (Mycobacterial Growth Indicator Tube) and Lowenstein Jensen (LJ) culture. Cultures positive by either MGIT or LJ were used for proportional DST (DST-LJ) (RIF 40 μg/ml and INH 0.2 μg/ml). DST profiles on MODS and LJ were compared. Discrepant results were resolved by multiplex allele specific PCR (MAS-PCR). Seven hundred and nine TB suspects/samples were enrolled into the study, of which 300 samples with DST profiles available from both MODS and

Stimulated Raman scattering (SRS) microscopy allows label-free chemical imaging based on vibrational spectroscopy. Narrowband excitation with picosecond lasers creates the highest signal levels and enables imaging speeds up to video-rate, but it sacrifices chemical specificity in samples with overlapping bands compared to broadband (multiplex) excitation. We develop a rapidly tunable picosecond optical parametric oscillator with an electro-optical tunable Lyot filter, and demonstrate multicolor SRS microscopy with synchronized line-by-line wavelength tuning to avoid spectral artifacts due to sample movement. We show sensitive imaging of three different kinds of polymer beads and live HeLa cells with moving intracellular lipid droplets.. © 2013 Optical Society of America. Full Article , PDF Article ...

In light microscopy an object is illuminated with a lamp or a laser and the transmitted, reflected or fluorescent light is detected to get information on the object. In the project two approaches will be developed to study small particles and their interaction with living cells: Light Sheet Fluorescence Microscopy and Complex Beam Scattering Microscopy. In standard fluorescence microscopy the entire sample is illuminated and there is an important background arising from fluorescent material that is out of focus. In this project we will illuminate the sample with a sheet of light that coincides with the focal plane of the microscope, so that only that part of the sample that is in focus is illuminated. This can be realized with an optical fiber or by illuminating through the microscope objective lens onto an inclined mirror mounted on the sample holder. These solutions should be inexpensive and compatible with existing microscopes. For the study of small sub-resolution features in a sample we ...

Synthesis of new proteins, a key step in the central dogma of molecular biology, has been a major biological process by which cells respond rapidly to environmental cues in both physiological and pathological conditions. However, the selective visualization of a newly synthesized proteome in living systems with subcellular resolution has proven to be rather challenging, despite the extensive efforts along the lines of fluorescence staining, autoradiography, and mass spectrometry. Herein, we report an imaging technique to visualize nascent proteins by harnessing the emerging stimulated Raman scattering (SRS) microscopy coupled with metabolic incorporation of deuterium-labeled amino acids. As a first demonstration, we imaged newly synthesized proteins in live mammalian cells with high spatial-temporal resolution without fixation or staining. Subcellular compartments with fast protein turnover in HeLa and HEK293T cells, and newly grown neurites in differentiating neuron-like N2A cells, are clearly ...

Metabolic fingerprinting provides valuable information on the physiopathological states of cells and tissues. Traditional imaging mass spectrometry and magnetic resonance imaging are unable to probe the spatial-temporal dynamics of metabolites at the subcellular level due to either lack of spatial r …

Fourier domain Optical coherence microscopy (FDOCM) offers excellent sensitivity and high axial resolution to image the structure of biological tissue. The depth information is extracted in parallel and allows very high volume acquisition rates. The present system uses a diffractionless beam, produced with an axicon lens, to achieve high lateral resolution all while maintaining an extended depth of field (xf). The xfOCM signal reveals the spatial distribution of changes of the refractive index in the sample that scatter the incident light. To identify and validate the functionality of the observed structures can proof difficult. In this work the xfOCM setup was interfaced with a fluorescent lifetime imaging (FLIM) system, working in the Fourier domain and measuring the phase offset between the modulated excitation signal and the returned fluorescence intensity. Both the fluorescence amplitude and lifetime are retrieved. The amplitude contains important information due to the selective labeling ...

Circadian rhythms are endogenous, entrainable oscillations of physical, mental and behavioural processes in response to local environmental cues such as daylight, which are present in the living beings, including humans. Circadian rhythms have been related to cardiovascular function and pathology. However, the role that circadian clock genes play in heart development and function in a whole animal in vivo are poorly understood. The Drosophila cryptochrome (dCry) is a circadian clock gene that encodes a major component of the circadian clock negative feedback loop. Compared to the embryonic stage, the relative expression levels of dCry showed a significant increase (|100-fold) in Drosophila during the pupa and adult stages. In this study, we utilized an ultrahigh resolution optical coherence microscopy (OCM) system to perform non-invasive and longitudinal analysis of functional and morphological changes in the Drosophila heart throughout its post-embryonic lifecycle for the first time. The Drosophila

Buy Handbook of Full-Field Optical Coherence Microscopy by Arnaud Dubois (9789814669160) from Boomerang Books, Australias Online Independent Bookstore

TY - JOUR. T1 - Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer. AU - Rappaz, Benjamin. AU - Barbul, Alexander. AU - Emery, Yves. AU - Korenstein, Rafi. AU - Depeursinge, Christian. AU - Magistretti, Pierre. AU - Marquet, Pierre. PY - 2008/10/1. Y1 - 2008/10/1. N2 - Red blood cell (RBC) parameters such as morphology, volume, refractive index, and hemoglobin content are of great importance for diagnostic purposes. Existing approaches require complicated calibration procedures and robust cell perturbation. As a result, reference values for normal RBC differ depending on the method used. We present a way for measuring parameters of intact individual RBCs by using digital holographic microscopy (DHM), a new interferometric and label-free technique with nanometric axial sensitivity. The results are compared with values achieved by conventional techniques for RBC of the same donor and previously published figures. A DHM ...

TY - GEN. T1 - Cell death detection and ionic homeostasis monitoring with digital holographic microscopy. AU - Pavillon, Nicolas. AU - Kühn, Jonas. AU - Jourdain, Pascal. AU - Depeursinge, Christian. AU - Magistretti, Pierre. AU - Marquet, Pierre. PY - 2011/7/25. Y1 - 2011/7/25. N2 - Digital holographic microscopy is an interferometric technique enabling the measurement of the quantitative phase shifts induced by cell bodies. We correlate the phase signal measured on neurons with calcium imaging measured by fluorescence on cells loaded with Fluo-4, to monitor responses to glutamate challenges, which provoke well-known calcium increases through activation of various membrane receptors. A very good correspondence can be identified between the two signals, showing the links between the phase signal, being a measure of the intracellular dilution, and the calcium concentration within cells. We then check cell viability by employing propidium iodide (PI), a fluorescent indicator relying on the cell ...

TY - JOUR. T1 - Correction of phase-shifting error in wavelength scanning digital holographic microscopy. AU - Zhang, Xiaolei. AU - Wang, Jie. AU - Zhang, Xiangchao. AU - Xu, Min. AU - Zhang, Hao. AU - Jiang, Xiangqian. PY - 2018/5. Y1 - 2018/5. N2 - Digital holographic microscopy is a promising method for measuring complex micro-structures with high slopes. A quasi-common path interferometric apparatus is adopted to overcome environmental disturbances, and an acousto-optic tunable filter is used to obtain multi-wavelength holograms. However, the phase shifting error caused by the acousto-optic tunable filter reduces the measurement accuracy and, in turn, the reconstructed topographies are erroneous. In this paper, an accurate reconstruction approach is proposed. It corrects the phase-shifting errors by minimizing the difference between the ideal interferograms and the recorded ones. The restriction on the step number and uniformity of the phase shifting is relaxed in the interferometry, and the ...

We believe that these imaging modalities have many potential medical applications for corneal/ocular surface imaging when the technology is further developed. Chronic topical glaucoma therapy has been associated with corneal damage characterized by the loss of certain corneal cell types as well as invasion of inflammatory cells. 48,49 CARS/TPAF would allow us to observe the number and type of corneal epithelial cells, which could determine the progression of corneal surface disease to better aid physician treatment. CARS/TPAF also has application in the area of corneal surgery, since this technology could be used to observe cell number and metabolic state (NAD[P]H level) at the site of a healing wound. Furthermore, CARS/TPAF can image at the level of the corneal endothelium to monitor cell health before and after cataract surgery. A combined CARS/TPAF system has recently been used to show the differences in lipid structures between in healthy and psoriasis-affected human skin. 50 By the same ...

Objectives: To evaluate inter-observer agreement for microscopic measurement of inflammation in synovial tissue using manual quantitative, semiquantitative and computerised digital image analysis.. Methods: Paired serial sections of synovial tissue, obtained at arthroscopic biopsy of the knee from patients with rheumatoid arthritis (RA), were stained immunohistochemically for T lymphocyte (CD3) and macrophage (CD68) markers. Manual quantitative and semiquantitative scores for sub-lining layer CD3+ and CD68+ cell infiltration were independently derived in 6 international centres. Three centres derived scores using computerised digital image analysis. Inter-observer agreement was evaluated using Spearmans Rho and intraclass correlation coefficients (ICCs).. Results: Paired tissue sections from 12 patients were selected for evaluation. Satisfactory inter-observer agreement was demonstrated for all 3 methods of analysis. Using manual methods, ICCs for measurement of CD3+ and CD68+ cell infiltration ...

TY - GEN. T1 - A virtual microscope system for histology learning in education. AU - Chen, Lih Shyang. AU - Lay, Young Jinn. AU - Yang, Chih Ching. AU - Chang, Shu Han. PY - 2015/10/12. Y1 - 2015/10/12. N2 - Traditional microscopes are used as basic tools for histology education and have caused many problems in schools. Due to the advances of information technology, some virtual microscopy systems (VM systems) have been developed over years for educational purposes. The VM systems have been proven in the literature to be useful and helpful for students learning. However, currently, most VM systems just try to mimic the physical microscopes and there is still a lot of room to improve their usefulness for educational purposes. In this paper, we discuss what kinds of features and technologies a VM system should have in order to support various pedagogical strategies. We discuss the features of an ideal virtual microscope system that are needed to make the histology learning more effective and ...

Live Blood Cell Darkfield Microscopy Analysis Forum live blood microscopy or Live Blood Analysis (LBA), sometimes called Live Blood Cell Analysis, is the high resolution microscopic observation of live blood cells in a dark field - dark field blood test, a common technique in microbiology called dark field microscopy. Two drops of blood under a specialized high powered ultra-dark field microscope, reveals anomalies in the blood, that relate to deficiencies in nutrients, dysfunctional bodily systems, toxins and dysbiosis of the human body. discussion in the Forum about darkfield microscopes, darkfield, darkfield microscopy, Live Cell Blood Analysis. live blood analysis course: study dark field microscopy blood analysis with live blood darkfield microscopy. Dark field microscopy blood was used by Prof. Enderlein in Germany with an live blood microscope, darkfield blood analysis or darkfield live blood analysis can you study in our live blood analysis courses here and online. Our sources are from ...

Whole slide imaging is Food and Drug Administration-approved for primary diagnosis in the United States of America; however, relatively few pathology departments in the country have fully implemented an enterprise wide digital pathology system enabled for primary diagnosis. Digital pathology has significant potential to transform pathology practice with several published studies documenting some level of diagnostic equivalence between digital and conventional systems. However, whole slide imaging also has significant potential to disrupt pathology practice, due to the differences in efficiency of manipulating digital images vis-à-vis glass slides, and studies on the efficiency of actual digital pathology workload are lacking. Our randomized, equivalency and efficiency study aimed to replicate clinical workflow, comparing conventional microscopy to a complete digital pathology signout using whole slide images, evaluating the equivalency and efficiency of glass slide to whole slide image reporting,

Second harmonic generation (SHG) microscopy has become an important tool for minimally invasive biomedical imaging. However, differentiation of different second harmonic generating species is mainly provided by morphological features. Using excitation polarization-resolved SHG microscopy we determined the ratios of the second-order susceptibility tensor elements at single pixel resolution. Mapping the resultant ratios for each pixel onto an image provides additional contrast for the differentiation of different sources of SHG. We demonstrate this technique by imaging collagen-muscle junction of chicken wing ...

This paper present a novel approach to perform the tomography of biological specimen based on Digital Holographic Microscopy (DHM). A hologram results from the interference between a reference wave and an object wave reflected from or transmitted through a sample. In the hologram, both amplitude and phase of the field transmitted through the object are registered. In DHM, the object field is recovered when the hologram is processed by a digitally computed replica of the reference wave, allowing quantitative measurement of both phase and amplitude. Phase measurements provide high accuracy optical path length measurements across the specimen along the optical axis. To proceed to a tomographic reconstruction of the refractive index of the sample based on this quantitative phase measurement, such 2-dimentionnal data must be recorded for different sample orientations covering an angle of 180° to cover all the object spatial frequencies in the reciprocal space. The representation of the data in ...

The origin of the FOM conference series were the three-dimensional imaging capabilities of confocal microscopes together with the associated 3D image processing methodologies that developed in the mid 80s. The technology for optical microscopy has been evolved rapidly with the development of opto electronic technologies such as femtosecond lasers and highly sensitive photo-detectors, together with a wide variety of new fluorescent staining methodologies and digital image analysis routines for 3D and live cell 4D data sets. Nonlinear optical microscopy including multi-photon excited fluorescence microscopy, time-resolved micro-spectroscopy, and second-harmonic and coherent anti-Stokes Raman are making imaging of molecular compositions possible. High-resolution optical microscopy, including near-field scanning optical microscopy, four-pi confocal microscopy, and coherence probe microscopy also constitute a major research topic that progresses optical microscopy towards nanoscopy. These timely ...

FISH digital microscopy. Technician studying FISH (Fluorescence In Situ Hybridization) images obtained using an Ikoniscope, a fully automated digital microscopy machine. FISH is a cytogenetic technique used to detect and localize the presence or absence of specific DNA (deoxyribonucleic acid) sequences on chromosomes. Here it is being used as part of a breast cancer screening process to determine the presence of HER-2 (Human Epidermal growth factor Receptor 2), a protein giving higher aggressiveness in breast cancers. - Stock Image C003/0175

Dr. Matthias Meier (IMTEK, University of Freiburg) Raman microscopy is a powerful optical technology able to insight into the organic and inorganic chemical composition of living cells. By providing specific information with high spatial resolution about vibrational energy levels of chemical bonds and molecules, Raman microscopy can fingerprint metabolic states of cells. On the other hand microfluidic large-scale integration technology has demonstrate to control precisely the chemical microenvironment of hundreds of cell cultures in parallel with over weeks. In this funded BIOSS project we aim to couple microfluidic chip technology and raman microscopy to study lipid signaling between and in primary human adipocytes. Lipid droplets within adipocytes are the largest energy reservoir in the human body. External hormone, and nutrient availability tightly regulate the build-up (lipogenesis) and break down (lipolysis) of the lipid droplets. Disruption of the regulatory signal network controlling ...

Digital holographic microscopy (DHM) is a well-known powerful method allowing both the amplitude and phase of a specimen to be simultaneously observed. In order to obtain a reconstructed image from a hologram, numerous calculations for the Fresnel di

Waveguide Evanescent Field Scattering (WEFS) microscopy is introduced as a new and simple tool for label-free, high contrast imaging of bacteria and bacteria sensors. Bacterial microcolonies and single bacteria were discriminated both by their bright field images and by their evanescent scattering intensity. By comparing bright field images with WEFS images, the proportion of planktonic: sessile (i.e., floating: attached) bacteria were measured. Bacteria were irradiated with UV light, which limited their biofilm forming capability. A quantitative decrease in attachment of individual, sessile bacteria and in attached, microcolony occupied areas was easily determined within the apparent biofilms with increasing UV dose. WEFS microscopy is an ideal tool for providing rapid quantitative data on biofilm formation.

DESCRIPTION (provided by applicant): Fluorescence imaging is the current major optical method for cell and tissue analysis. It relies on the use of fluorescent molecular probes which are either expressed by genes which have been modified, or are tagged onto molecules of interest. The modification can interfere with normal function and hamper the interpretation of experimental results. Furthermore, the fluorophores themselves are subject to photobleaching which results in limited imaging time and prevents long term studies. Coherent anti-Stokes Raman scattering (CARS) microscopy is a nonlinear optical imaging technique that allows high-speed vibrational imaging of molecules. CARS microscopy offers several unique advantages: (a) CARS imaging is label-free andnon-destructive. (b) The nonlinear dependence on excitation intensity ensures that the CARS signal is only generated in the focal center, providing an inherent sub-micron 3D spatial resolution. (c) CARS imaging offers intrinsic chemical ...

Professor Xiaoliang Sunney Xie (Chinese: 谢晓亮; born 1962 in Beijing, China) is considered a founding father of single-molecule biophysical chemistry and single-molecule enzymology. He received his B.Sc. in Chemistry from Peking University in 1984, and his Ph.D. in Physical Chemistry in 1990 from University of California at San Diego. After a brief postdoctoral appointment at University of Chicago, he joined Pacific Northwest National Laboratory, where he rose from Senior Research Scientist to Chief Scientist. In 1998, he became the first tenured professor at Harvard University among Chinese Scholars who came to the United States since the Reform in China. As a pioneer of single-molecule biophysical chemistry, coherent Raman scattering microscopy, and single-cell genomics, he made major contributions to the emergence of these fields. Furthermore, he has made significant advances on medical applications of label-free optical imaging and single-cell genomics. In particular, his inventions in ...

ISSAQUAH, Wash., August 16th, 2010 - Applied Precision, along with Indiana University, are excited to announce the installation of a new DeltaVision OMXimaging system in the Light Microscopy Imaging Center in Bloomington, Indiana.. The DeltaVision OMX is a ground-breaking, three dimensional, super-resolution microscopy system that more than doubles the optical resolution of traditional light microscopy. This super-resolution capability is especially important to cell biologists and microbiologists seeking to study objects and events that lie beyond the limits of conventional microscopy methods.. We are thrilled to have Indiana University as a client for our pioneering DeltaVision OMX system, says Joe Victor, president and CEO of Applied Precision. Dr. Claire Walczak and the team of researchers at IU are performing the type of cutting-edge research that drives our companys mission of improving lives through the use of advanced imaging solutions.. The Light Microscopy Imaging Center is lead ...

Spectroscopic coherent Raman imaging (CRI) methods allow label-free, chemically specific imaging of materials and biological systems, and are opening up many exciting possibilities for understanding phenomena in these systems. I will briefly introduce broadband spectroscopic coherent anti-Stokes Raman scattering (BCARS) microscopy, discussing some of the key concepts that make this method practical. I will also present selected application examples from studies on C. elegans metabolism and human cytomegalovirus infection that highlight the utility of BCARS for discovering new biology through in-depth characterization of highly complex biological systems.. © 2019 The Author(s). PDF Article ...

In the pharmaceutical industry, novel analytical techniques are required to gain important insights about new drug candidates and their formulations as early as possible. This information can be used to develop more efficient, safe and also economically profitable medicines. Microscopy techniques can be used for example to follow the fate of nanoparticles in cells and tissues, but also to monitor the changes in solid-state forms of drug molecules during drug development and storage. The overall aim of the Thesis was to evaluate the capability of non-linear optical imaging, especially coherent anti-Stokes Raman scattering (CARS), second harmonic generation and sum-frequency generation (SHG and SFG) microscopies, in pharmaceutical applications including imaging of live cells, nanoparticle cellular uptake and pharmaceutical solid-state analysis. First, the capability of CARS microscopy to image live cell cultures on pharmaceutically relevant membrane inserts was evaluated. It was found that, ...

Explore the basics of automated digital microscopy systems, including: a brief history of the microscope, the current state of the automated digital microscope, microscope objective types and automated XY stage motion and fluorescence imaging with Dover Motion.

MICHIGAN STATE (US) - Using laser microscopes that deploy rapid, ultra-short pulses to identify molecules, doctors may soon have the tools to perform painless skin cancer biopsies.. To test for skin cancer, patients today must endure doctors cutting away a sliver of skin and anxiously await the lab results.. Smart lasers allow us to selectively excite compounds-even ones with small spectroscopic differences, says Marcos Dantus, a chemistry professor at Michigan State University. We can shape the pulse of the lasers, excite one compound or another based on their vibrational signatures, and this gives us excellent contrast.. Dantus and co-author Sunney Xie of Harvard University report their findings in the journal Nature Photonics.. In the past, researchers could approach this level of contrast by introducing fluorescent compounds. With the breakthrough using stimulated Raman scattering microscopy, fluorescent markers are unnecessary.. Label-free molecular imaging has been the holy grail in ...

Many of the biological functions of a cell are dictated by the intricate motion of proteins within its membrane over a spatial range of nanometres to tens of micrometres and time intervals of microseconds to minutes. This rich parameter space is not accessible by fluorescence microscopy, but it is within reach of interferometric scattering (iSCAT) particle tracking. However, as iSCAT is sensitive even to single unlabelled proteins, it is often accompanied by a large speckle-like background, which poses a substantial challenge for its application to cellular imaging. Here, we employ a new image processing approach to overcome this difficulty and demonstrate tracking of transmembrane epidermal growth factor receptors with nanometre precision in all three dimensions at up to microsecond speeds and for durations of tens of minutes. We provide examples of nanoscale motion and confinement in ubiquitous processes such as diffusion in the plasma membrane, transport on filopodia and rotational motion during

S.-W. Chu, K. Fujita, B. Kemper, N. Pavillon, N. I .Smith, Trends in label-free imaging, Opt. Commun. 422, 1-2 (2018).. L. Brandt, K. Brinker, B. Kemper, Automatic Computation of Biophysical Cell Parameters in Digital Holographic Microscopy Images, Proc. BIOSTEC 2018 5, 431-437 (2018). B. Kemper, Digital holographic microscopy for toxicity testing and cell culture quality control (Invited Paper), Proc. SPIE 10503, 105031J (2018). B. Kemper, S. Ketelhut, Multi-spectral digital holographic microscopy for enhanced quantitative phase imaging of living cells, Proc. SPIE 10503, 1050313 (2018). J. Schnekenburger, S. Ketelhut, B. Kemper, A novel multimodal optical concept for the detection of bacteria and microplastics in the environment, Proc. SPIE 10491-39 (2018). L. Kastl, S. Ketelhut, B. Kemper, J. Schnekenburger, Quantitative phase imaging for enhanced assessment of optomechanical cancer cell properties, Proc. SPIE 10503, 105032R (2018).. M. Rezaei, K. Friedrich, B. Kemper, O. Brendel, ...

PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.

On the instrumentation side, we develop multimodal nonlinear microscopy and micro-spectroscopy technologies using ultra-short laser pulses (from 10 fs to ps), possibly synchronized (using electronic synchronization or OPOs). Special attention is given to coherent Raman scattering modalities such as coherent anti-Stokes Raman scattering (CARS ) and stimulated Raman scattering (SRS) for label-free vibrational microscopy and micro-spectroscopy.. ...

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Nonlinear optical microscopy (NOM) has become increasingly popular in biomedical research in recent years with the developments of laser sources, contrast mechanisms, novel probes and etc. One of the advantages of NOM over the linear counterpart is the ability to image deep into scattering tissues or even on the whole animals. This is due to the adoption of near-infrared excitation that is of less scattering than visible excitation, and the intrinsic optical sectioning capability minimizing the excitation background beyond focal volume. Such an advantage is particularly prominent in two-photon fluorescence microscopy compared to one-photon fluorescence microscopy. In addition, NOM may provide extra molecular information (e.g. second harmonic generation and third harmonic generation) or stronger signal (e.g. stimulated Raman scattering and coherent anti-Stokes Raman scattering compared to spontaneous Raman scattering), because of the nonlinear interaction between strong optical fields and ...

Light Microscopy and Resolution -- Super-Resolution Microscopy: Principles, Techniques, and Applications -- Foundations of STED Microscopy -- Application of Real-Time STED Imaging to Synaptic Vesicle Motion -- Photoactivated Localization Microscopy for Cellular Imaging -- Data Analysis for Single-Molecule Localization Microscopy -- Super-Resolution Fluorescence Microscopy Using Structured Illumination -- Application of Three-Dimensional Structured Illumination Microscopy in Cell Biology: Pitfalls and Practical Considerations -- Scanning Near-Field Optical Microscopy for Investigations of Bio-Matter -- Atomic Force Microscopy of Living Cells -- X-Ray Microscopy for Neuroscience: Novel Opportunities by Coherent Optics -- Nonlinear Optics Approaches Towards Sub-Diffraction Resolution in CARS Imaging -- Photo-Oxidation Microscopy: Bridging the Gap Between Fluorescence and Electron Microscopy -- Requirements for Samples in Super-Resolution Fluorescence Microscopy -- Probing Biological Samples in ...

Live Blood Cell Darkfield Microscopy Analysis Forum live blood microscopy or Live Blood Analysis (LBA), sometimes called Live Blood Cell Analysis, is the high resolution microscopic observation of live blood cells in a dark field - dark field blood test, a common technique in microbiology called dark field microscopy. Two drops of blood under a specialized high powered ultra-dark field microscope, reveals anomalies in the blood, that relate to deficiencies in nutrients, dysfunctional bodily systems, toxins and dysbiosis of the human body. discussion in the Forum about darkfield microscopes, darkfield, darkfield microscopy, Live Cell Blood Analysis. live blood analysis course: study dark field microscopy blood analysis with live blood darkfield microscopy. Dark field microscopy blood was used by Prof. Enderlein in Germany with an live blood microscope, darkfield blood analysis or darkfield live blood analysis can you study in our live blood analysis courses here and online. Our sources are from ...

TY - JOUR. T1 - High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization. AU - Holden, Seamus J.. AU - Pengo, Thomas. AU - Meibom, Karin L.. AU - Fernandez, Carmen Fernandez. AU - Collier, Justine. AU - Manley, Suliana. N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.. PY - 2014/3/25. Y1 - 2014/3/25. N2 - We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We observed that FtsZ predominantly localizes as a patchy midcell band, and only rarely as a continuous ring, supporting a model of Z-ring organization whereby FtsZ protofilaments are randomly distributed within the band and interact only weakly. We found evidence for a previously ...

This instrument had a noise floor of just 1 pm/√Hz, allowing the atomic-scale vibrations of interest to the Micromechanics group to be measured. For more information about this project, my final thesis is available here, and the abstract is reprinted below.. Design and Implementation of a Fiber Optic Doppler Optical Coherence Microscopy System for Cochlear Imaging. In this thesis, the design and implementation of a fiber optic Doppler optical coherence microscopy (FO-DOCM) system for cochlear imaging applications is presented. The use of a fiber optic design significantly reduces system size and complexity and the construction of a novel alignment and micropositioning apparatus increases ease of use for the researcher performing the imaging. To enable precise measurements of tissue motion, a time domain DOCM approach is used, utilizing an acousto-optic modulator (AOM) based optical heterodyne system to generate a stationary interference carrier frequency. By referencing this interference ...

Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic microscopy (DHM). This interferometric and dye-free technique, possessing nanometric axial and microsecond temporal sensitivities enables to measure cell membrane fluctuations (CMF) on the whole cell surface. DHM acquisition is combined with a model which allows extracting the membrane fluctuation amplitude, while taking into account cell membrane topology. Uneven distribution of CMF amplitudes over the RBC surface is observed, showing maximal values in a ring corresponding to the highest points on the RBC torus as well as in some scattered areas in the inner region of the RBC. CMF amplitudes of 35.9+/-8.9 nm and 4.7+/-0.5 nm (averaged over the cell surface) were determined for normal and ethanol-fixed RBCs, respectively.

Research in the Fu group focuses on developing novel optical spectroscopy and imaging techniques to investigate the spatial-temporal dynamics of living biological systems at single cell resolution. In particular, we are interested in using label-free spectroscopic imaging approaches such as stimulated Raman scattering microscopy to study the cellular mechanism of complex diseases such as cancer and metabolic disorders. We draw on expertise from multiple disciplines including analytical chemistry, ultrafast spectroscopy, imaging science, optical engineering, bioinstrumentation, cell biology, and systems biology.. Label-free optical microscopy. Optical microscopy, especially fluorescence microscopy, is instrumental in unraveling the intricate biological processes that happen inside living cells. However, most biomolecules in the cell do not fluorescence and visualizing those molecules requires fluorescent tags, which may often be unavailable or perturbative to their biological functions. We ...

Two-photon polymerization (TPP) is an enabling technology that allows fast prototyping of parts with sub-100 nm resolution. Due to its ability to fabricate microstructures with arbitrary three-dimensional geometries, TPP has been employed in diverse fields such as nanophotonics, microelectronics, microelectromechanical systems, and microfluidics. However, no information is available to date that microscopically correlates the experimental conditions used in TPP with the properties of the ultimate microstructure. We present a study where the distribution of polymer cross-linking in three-dimensional microstructures fabricated by TPP is visualized by means of coherent anti-Stokes Raman scattering (CARS) microscopy. The characterization of the microstructures based on the acquired images permits rational optimization of the TPP process

The formation of the basoapical polarity axis in epithelia is critical for maintaining the homeostasis of differentiated tissues. Factors that influence cancer development notoriously affect tissue organization. Apical polarity appears as a specific tissue feature that, once disrupted, would facilitate the onset of mammary tumors. Thus, developing means to rapidly measure apical polarity alterations would greatly favor screening for factors that endanger or protect the breast epithelium. A Raman scattering based platform was used for label-free determination of apical polarity in live breast epithelial structures (acini) produced in three-dimensional cell culture. The coherent anti-Stokes Raman scattering signal permitted the visualization of the apical and basal surfaces in the equatorial plane of an acinus. Raman microspectroscopy subsequently revealed that in polarized acini lipids were more ordered at the apical membranes compared to basal membranes, and that an inverse situation occurred in ...

The MV4000 multiprobe scanned probe microscopy systems manufactured by !%Nanonics Imaging Ltd.%! independently scan four probes with ultralow noise in

article{2da3f822-7032-422c-900e-e4a29654ecd9, abstract = {,p,Pure rotational coherent anti-Stokes Raman scattering (CARS) experiments have been performed in acetylene for temperatures ranging from 294 to 582 K, and in mixtures of acetylene and nitrogen in the mole fraction range of 0.06-0.32 for acetylene at room temperature. The experimental spectra are evaluated by a least-square fitting to libraries of theoretically calculated spectra using two different Raman linewidth models, one with and one without dependence on the rotational quantum number J. It is found that a J-dependent model is favourable, both regarding temperature measurements in pure acetylene, and simultaneous acetylene concentration and temperature measurements in different mixtures of acetylene and nitrogen. For the temperature measurements performed in pure acetylene the temperature inaccuracy is generally less than 2% when the J-dependent model for the Raman linewidths is used. It is found that fitting the value of the ...

We have developed a multiphoton microscopy (MPM) system using a 12-fs Ti:sapphire laser with adjustable dispersion precompensation in order to examine the impact of pulse duration on nonlinear optical signals. The efficiencies of two-photon-excited fluorescence (TPEF) and second harmonic generation (SHG) were studied for various pulse durations, measured at the sample, ranging from approximately 400 fs to sub-20 fs. Both TPEF and SHG increased proportionally to the inverse of the pulse duration for the entire tested range. Because of improved signal-to-noise ratio, sub-20-fs pulses were used to enhance MPM imaging depth by approximately 160%, compared to 120-fs pulses, in human skin.. ...

Raman spectroscopy has numerous applications in the field of biology. One such application is the simultaneously measurement of the concentration of multiple biochemical components in low volume aqueous mixtures, for example, a single drop of blood serum. Over twenty years ago, it was shown for the first time that it was possible to estimate the concentration of glucose, urea, and lactic acid in mixture by combining Raman Spectroscopy with Partial Least Squares Regression analysis. This was followed by numerous contributions in the literature designed to increase the number of components and reduce the limits of concentration that could be simultaneously measured using Raman spectroscopy, by developing various optical architectures to maximise the signal to noise ratio. The aim of this paper is to demonstrate the potential of a confocal Raman microscopy system for multicomponent analysis for the case of physiologically relevant mixtures of glucose, urea, and lactic acid.. ...

BRANFORD, Conn., Oct. 6, 2011 /PRNewswire/ - HistoRx, the leader in quantitative immunohistochemistry, advances its proprietary position in digital pathology through the recent issuance of US patents covering key features necessary for achievement of reproducible, standardized image analysis results. The U.S. Patent and Trademark Office has granted two new patents protecting the companys methods for standardization of digital microscopy instruments, methods required to generate high-quality reproducible clinical diagnostic data that precisely relates to biomarker concentrations in tissue sections.. For patient tissue specimens stained for a biomarker such as HER2, HistoRx believes that physicians and patients expect the same quantitative result regardless of the digital microscopy instrument used. To achieve this, HistoRx evaluated sources of variability in digital scanning microscopy instrumentation that affect results. One source of variability is the light source; a second is the path ...

RNTCP Lab Technician/ Sputum Microscopist Recruitment 2018, RNTCP Lab Technician/ Sputum Microscopist Syllabus, RNTCP Lab Technician/ Sputum Microscopist Previous Papers, Sample Papers - Revised National Tuberculosis Control Programme, RNTCP Lab Technician/ Sputum Microscopist Recruitment 2018, Syllabus, Sample Paper

The objectives for these two categories are distinct and are marked on the barrel of the objective. The objective shown below is for reflected light. The BD indicates that this microscope objectives is intended for brightfield / darkfield illumination, a standard technique in reflected light microscopy. (BD reflected light objectives also usually have a larger thread mount and barrel to accommodate the additional light path. These lenses will not fit the standard nosepiece). EPI indicates that this microscope objective is used for epi-illumination. Immediately after the infinity symbol there is /0 - this means the objective is intended to be used without a cover slip. This is also the main difference in the design of reflected light microscope objectives ...

Crystals of congruently melting noncentrosymmetric (NCS) mixed alkali metal nitrate, Rb2Na(NO3)3, have been grown through solid state reactions. The material possesses layers with NaO8 hexagonal bipyramids and NO3 triangular units. Rb+ cations are residing in the interlayer space. Each NaO8 hexagonal bipyramid shares its corners and edges with two and three NO3 units, respectively, in order to fulfill a highly dense stacking in the unit cell. The NaO8 groups share their six oxygen atoms in equatorial positions with three different NO3 groups to generate a NaO6-NO3 layer with a parallel alignment. The optimized arrangement of the NO3 groups and their high density in the structure together produce a strong second-harmonic generation (SHG) response. Powder SHG measurements indicate that Rb2Na(NO3)3 has a strong SHG efficiency of five times that of KH2PO4 (KDP) and is type I phase-matchable. The calculated average nonlinear optical (NLO) susceptibility of Rb2Na(NO3)3 turns out to be the largest value among

Interaction of zoospores of Ulva linza with cationic, arginine-rich oligopeptide self-assembled monolayers (SAMs) is characterized by rapid settlement. Some spores settle (ie permanently attach) in a normal manner involving the secretion of a permanent adhesive, retraction of the flagella and cell wall formation, whilst others undergo pseudosettlement whereby motile spores are trapped (attached) on the SAM surface without undergoing the normal metamorphosis into a settled spore. Holographic microscopy was used to record videos of swimming zoospores in the vicinity of surfaces with different cationic oligopeptide concentrations to provide time-resolved insights into processes associated with attachment of spores. The data reveal that spore attachment rate increases with increasing cationic peptide content. Accordingly, the decrease in swimming activity in the volume of seawater above the surface accelerated with increasing surface charge. Three-dimensional trajectories of individual swimming ...

Confocal Raman microscopy can identify particles in the 5-50 ?m range and can bridge the gap between micro-FT-IR and SEM-EDS analyses.

0071]In an alternate embodiment, the invention comprises a developable microscope slide coverslip which is constructed from a developable liquid microscope slide coverslip media that is spread over the microscope slide at the end of testing to preserve the biological specimen disposed thereon without the need for a separate glass or plastic microscope slide coverslip being attached to the slide via a common mounting medium. The developable microscope slide coverslip can be made by the liquid microscope slide coverslip media, of the present invention, that can be spread thinly over the biological specimen by a gap coater or similar applicator that can make a thin coating from the liquid media. The microscope slide coverslip media thus could be placed in front or rear of the gap coater and then the coater moved about the slide to lay down a specific thickness of media in accordance with the specific gap coater device. In one example, the gap coating device has a gap of 8 microns away from the ...

Olympus mplanfl 100x 0.90 bd microscope objective lens, mplanfl this item is in the category business & industrial\healthcare, lab & dental\medical/lab equipment attachments & accessories\microscope parts & accessories\microscope objectives. S arrival, and it has to be in the original condition as it was sent out ...

Introduction: HER2 gene amplification or overexpression occurs in 15% to 25% of breast cancers and has implications for treatment and prognosis. The most commonly used methods for HER2 testing are fluorescence in situ hybridization (FISH) and immunohistochemistry. FISH is considered to be the reference standard and more accurately predicts response to trastuzumab, but is technically demanding, expensive, and requires specialized equipment. In situ hybridization is required to be eligible for adjuvant treatment with trastuzumab in Australia. Bright-field in situ hybridization is an alternative to FISH and uses a combination of in situ methodology and a peroxidase-mediated chromogenic substrate such as diaminobenzidine [chromogenic in situ hybridization (CISH)] or multimer technology coupled with enzyme metallography [silver-enhanced in situ hybridization (SISH)] to create a marker visible under bright-field microscopy. CISH was introduced into diagnostic testing in Australia in October 2006. SISH ...

This graphics card normally helps computer gamers to have a great gaming experience. The researchers, however, use it to observe the smallest cell components in action - in real time and with a very high frame rate. „The image data can be reconstructed about twenty times faster than it would take on a PC, explains Rainer Heintzmann of the Leibniz Institute of Photonic Technology (Leibniz IPHT), who laid the foundations for the process of structured illumination in high-resolution microscopy back in 1998. Together with him, the Bielefeld research team led by Prof. Thomas Huser further expanded the technology of Super-Resolved Structured Illumination Microscopy (SR-SIM). In the fluorescence microscopic method SR-SIM, objects are irradiated with laser light using a special pattern. It excites special fluorescent molecules in the sample so that they emit light at a different wavelength. The microscopic image then shows this emitted light. It is first recorded in several individual images and then ...

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TY - JOUR. T1 - Atomic force microscopic detection enabling multiplexed low-cycle-number quantitative polymerase chain reaction for biomarker assays. AU - Mikheikin, Andrey. AU - Olsen, Anita. AU - Leslie, Kevin. AU - Mishra, Bud. AU - Gimzewski, James K.. AU - Reed, Jason. PY - 2014/7/1. Y1 - 2014/7/1. N2 - Quantitative polymerase chain reaction is the current golden standard for quantification of nucleic acids; however, its utility is constrained by an inability to easily and reliably detect multiple targets in a single reaction. We have successfully overcome this problem with a novel combination of two widely used approaches: target-specific multiplex amplification with 15 cycles of polymerase chain reaction (PCR), followed by single-molecule detection of amplicons with atomic force microscopy (AFM). In test experiments comparing the relative expression of ten transcripts in two different human total RNA samples, we find good agreement between our single reaction, multiplexed PCR/AFM data, ...

Project Description: The Kner and De La Fuente Labs are interested in using a novel 3D superresolution fluorescence microscopy approach to study nucleosome organization and the role of specific proteins in chromatin organization. The project involves the engineering of a new superresolution microscope, imaging of chromosomes, and biological questions that are important for in reproduction.. REU Student Role and Responsibility: The REU student will assist a graduate student in developing the microscope and in imaging experiments on samples form the De La Fuente lab. The student will assist in developing software to run the microscope and process the images, will help run the microscope to collect data, and will help prepare the samples for imaging.. Expected Outcome for REU student: The student will be trained in cutting-edge microscopy techniques and exposed to important questions in modern biology. Superresolution imaging of the nucleus is a hot topic. This project is expected to result in at ...

We present a novel detection scheme for Fourier domain optical coherence microscopy (FDOCM). A Bessel-like interference pattern with a strong central lobe was created with an axicon lens. This pattern was then imaged by a telescopic system into the sample space to obtain a laterally highly confined illumination needle, extending over a long axial range. For increased efficiency, the detection occurs decoupled from the illumination, avoiding a double pass through the axicon. Nearly constant transverse resolution of ~1.5&mgr;m along a focal range of 200&mgr;m with a maximum sensitivity of 105dB was obtained. A broad bandwidth Ti:Sapphire laser allowed for an axial resolution of 3&mgr;m in air, providing the nearly isotropic resolution necessary to access the microstructure of biological tissues. Together with the speed- and sensitivity-advantage of FDOCT, this system can perform in vivo measurements in a minimally invasive way. Tomograms of the mouse mammary gland and the mouse follicle, recorded ...

This project aims at improving the diagnosis of drowning. Diatoms in the water that enter the lungs will cross the alveolar-capillary barrier and will be distributed to, and trapped in the capillaries of remote tissues via the blood circulation. These diatoms can then be extracted from tissue samples and analyzed microscopically. In collaboration with Stockholm University the extracts are studied using conventional bright field microscopy and Scanning Electron Microscopy (SEM). We have established a method protocol based on protein K digestion of the organic material.

Fig. 1. Principle of super-resolution quantum dots triexiton imaging (QDTI). a. QDTI is realized by exciting semiconductor quantum dots QDot655 with 488 nm. As a consequence of multiexciton generation, three excitonic states with different fluorescence emission characteristics are generated. The emission of the triexciton is blue-shifted and can be spectrally separated. As a consequence of the three-photon absorption the resolution is increased ∼2-fold. b, c. Confocal fluorescence images of U373 cells, microtubule network stained by immunofluorescence using secondary antibodies labeled with QDot655. The triexciton channel (c) shows an increase in resolution and less contribution of out-of-focus light (optical sectioning). Figure adapted from [3].. B. Imaging of adult human neural crest derived stem cells by helium-ion microscopy. As described above, light microscopy is usually limited to resolve structures with sizes in the order of the wavelength and above. A few years ago helium-ion ...

STONY BROOK, N.Y. - The use of a new brain tumor-targeting contrast agent that differentiates between normal and cancer cells in conjunction with a high-powered microscopy system could potentially lead to a method of more precise neurosurgery for brain tumors, according to research paper published as a cover story in the December issue of Translational Oncology. Developed by researchers in the Department of Biomedical Engineering (BME) at Stony Brook University, the contrast agent adheres to a molecular marker of medulloblastoma, a form of brain cancer, and can be seen by the optical microscope system, also developed by the research team ...

Introduction Long-term drug delivery to the inner ear may be achieved by functionalizing cochlear implant (CI) electrodes with cells providing neuroprotective factors. However, effective strategies in order to coat implant surfaces with cells need to be developed. Our vision is to make benefit of electromagnetic field attracting forces generated by CI electrodes to bind BDNF-secreting cells that are labelled with magnetic beads (MB) onto the electrode surfaces. Thus, the effect of MB-labelling on cell viability and BDNF production were investigated. Materials and Methods Murine NIH 3T3 fibroblasts-genetically modified to produce BDNF-were labelled with MB. Results Atomic force and bright field microscopy illustrated the internalization of MB by fibroblasts after 24 h of cultivation. Labelling cells with MB did not expose cytotoxic effects on fibroblasts and allowed adhesion on magnetic surfaces with sufficient BDNF release. Discussion Our data demonstrate a novel approach for mediating enhanced ...

3.We have developed deep-tissue super-solution imaging (COOL, Confocal lOcalization deep-imaging with Optical cLearing), which combines advanced techniques of fluorescence protein labeling, confocal scanning microscopy, optical clearing, and localization microscopy to achieve 20-nm spatial resolution across a whole brain of Drosophila. Together these techniques are readily available for many biologists without the need of upgrading hardware, and provide unprecedented depth/resolution performance to three-dimensionally resolve densely entangled dendritic fibers from top to bottom in a complete Drosophila brain. The method not only paves the way toward whole-brain neural network studies, but also will be applicable to other high-resolution imaging in biological tissues. This was published in iScience 2019 (reference 3). Due to the contribution in the field of super-resolution microscopy, Prof. SW Chu was invited to write a News and Views article in a top optics journal, Light: Science and ...

According to TMR, the global life science microscopy devices market, which stood at US$1.1 bn in 2015, is projected to reach US$2.0 bn by the end of 2024. If these figures hold true, the global life science microscopy devices market will exhibit a CAGR of 6.5% between 2016 and 2024. By device type, the optical microscopes segment held the dominant share of 51.9% in the overall market in 2015. This segment is forecast to gain from the increasing demand for various types of optical microscopes such as inverted and fluorescence microscopes.. Regionally, North America dominated the global life science microscopy devices market with a share of 47.3% in 2015 and will continue doing so through the forecast period. Besides this, Asia Pacific exhibits high potential as the regional market for life science microscopy devices owing to the rising investment in R&D activities by biotechnology and pharmaceutical companies.. Browse Global Strategic Business ...

Sep 06, 2011 Able to meet need for working distances from 0.3-30 mm, apochromatic objectives are customized with high numerical apertures and long working distances for use with various sample sizes and applications. Lenses are also tailored to function at wavelengths from visible light at 390-750 nm to near infrared (NIR) at 700-1,400 nm. Depending on type of studies being done, 2-photon microscopy systems... Read More. ...

Stereoscopic on-screen surgical microscope systems are disclosed. One or more stereoscopic displays are provided which are corrected to the viewpoint of the participant or observer. A switching system which may be connected to the deflection yokes of a video monitor and a stereoscopic encoder, is employed to selectively provide up-down reversal, right-left reversal, and reversal of the images viewed by each eye. A stereoscopic video camera pod is employed to retrofit a surgical microscope to provide a field of view and magnification comparable to that obtained at the microscope eyepieces.

Imaging of biological matter across resolution scales entails the challenge of preserving the direct and unambiguous correlation of subject features from the macroscopic to the microscopic level. Here, we present a correlative imaging platform developed specifically for imaging cells in 3D under cryogenic conditions by using X-rays and visible light. Rapid cryo-preservation of biological specimens is the current gold standard in sample preparation for ultrastructural analysis in X-ray imaging. However, cryogenic fluorescence localization methods are, in their majority, diffraction-limited and fail to deliver matching resolution. We addressed this technological gap by developing an integrated, user-friendly platform for 3D correlative imaging of cells in vitreous ice by using super-resolution structured illumination microscopy in conjunction with soft X-ray tomography. The power of this approach is demonstrated by studying the process of reovirus release from intracellular vesicles during the early

The MH-2PM Fixed Position Microscope Objective Mount accommodates microscope objectives with the standard 0.800-36 RMS thread such as our M or L Series. 8-32 an...

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NKI: Are you mostly looking at mouse models right now?. Dr. Suleiman: In our recently accepted paper, we studied podocyte injury in three different mouse models. We included a small group of human tissue samples of FSGS, MCD and diabetic nephropathy in the study. We found that, similar to our mouse injury models, injured human podocytes show molecular changes that involve the motor molecules, myosin IIA.. As these results are in their early stages, I recently received a NEPTUNE (Nephrotic Syndrome Study Network) grant to study the biological significance of myosin IIA changes in human tissue samples. This study might allow us to find better diagnostic or prognostic tests for diseases such as MCD, FSGS and diabetic nephropathy.. NKI: So what youre saying is that you think one day we might be able to use the super-resolution microscopy technique to diagnose patients?. Dr. Suleiman: Yes, I can see that the super-resolution microscopy will be instrumental in the future to diagnose and predict the ...

X-ray microscopy requires radiation of extremely high quality. In order to obtain sharp images instrument and sample must stay absolutely immobile even at the nanometer scale during the recording. Researchers at the Technische ...

Supravital preparations of blood and haemopoietic tissues have been studied by interference contrast, phase contrast and conventional light microscopy to help identify the different categories of the smaller blood cells of lampreys. These methods were also used in conjunction with an examination of sectioned material and fixed blood films, to investigate macrophage activity by the blood cells and the liver Kupffer cells. The diameter of the smallest living blood cells was 4-5 ,mUm. When these produced extensive clotting fibres, particularly in the presence of foreign substances, they were obviously thrombocytes, whereas others, some of which contained prominent nucleoli, were considered to represent small lymphocytes and stem cells. Slightly larger cells (5-6 ,mUm), in which the nuclearcytoplasmic ratio was slightly lower and granules were being formed, represented the first stage in the formation of neutrophilic and eosinophilic granulocytes. The early members of the erythrocyte lineage could ...

Valentin Nägerl is a full professor of neuroscience and bio-imaging at the University of Bordeaux and heads a research group at the Interdisciplinary Institute for NeuroScience of the CNRS / University of Bordeaux.. He studied physics and medicine as an undergraduate at the University of Göttingen (1991-94) and at the University of California in Los Angeles (1994-95). He received a PhD in neuroscience from UCLA (1995-2000) in the lab of Istvan Mody. He then worked as a postdoc (2001-2004) and group leader (2005-2008) with Tobias Bonhoeffer at the Max Planck Institute of Neurobiology in Martinsried. In 2009 he received his habilitation in neuroscience from the Technical University of Munich (under Arthur Konnerth). In 2007 he worked with Stefan W. Hell (Nobel Prize for Chemistry 2014) at the Max Planck Institute for Biophysical Chemistry in Göttingen, where he learned STED microscopy.. In 2009 he set up his group in Bordeaux, working on synaptic plasticity in the mammalian brain using a ...

This last decade, many medical and technological efforts have been made to turn diagnostic pathology into a reliable medical practice taking advantage of multimedia systems for remote diagnostics, image banks, standards for images (DICOM Visible Light), reporting electronic forms, archiving systems and supporting certification and accreditation procedures. Unfortunately, these candidate materials could not be implemented on a large scale because the conventional microscope, as the central standalone imaging system, is still designed as a human viewing apparatus not suitable for the production of standardised digital images as demonstrated by the PRESS study (Bureau Communautaire des References). The e-scope project thus aims at making the fully digital microscope available for routine diagnostic practice with the key advantage of actually incorporating the standards for medical imaging, archiving and communication, in addition to enabling the certification and accreditation procedures set up for ...

Contact for confocal and other microscopes: Cosimo Arnesano ([email protected]). The mission of the Translational Imaging Center (TIC) is to develop new technologies for the imaging of biological structure and function. The technologies range from conventional light microscopy and laser scanning microscopy, to optical coherence tomography and Magnetic Resonance Imaging (MRI) microscopy.. As these technologies are refined, they are made available to the members of the USC research community. Thus, TIC serves dual, complementary roles as a research center and as a user facility.. For more information, visit bioimaging.usc.edu.. ...