I defended my PhD of Bioinformatics in December 2015 (CBIO, Mines ParisTech), after three years under the supervision of Thomas Walter, and the direction of Robert Barouki and Jean-Philippe Vert. During my PhD, I was interested in applying computer vision and machine learning methods to video-microscopy data. My research focused on the development of tools for automatic and quantitative characterization of cellular dynamic processes from video-microscopy data, following chemical exposure ...
The migration of lymphocytes through different lymphoid organs and other tissues of the body is a carefully controlled process that enables immune cells to encounter the cognate antigen and to disseminate memory and effector cells for immunologic surveillance (7). In this study, we have demonstrated, using intravital video microscopy, that activation of T lymphocytes by ConA leads to profound alterations in the sequential migration of these cells in the microcirculation of different lymphoid and nonlymphoid organs. The activated T cells either lose or exhibit a diminished capacity to enter Peyers patches and the spleen, whereas they exhibit a preferential distribution to the liver. A similar behavior of activated lymphocytes in lymph nodes or Peyers patches has been reported in previous studies, although these were in vivo homing studies that used51Cr-labeled lymphocytes or an in vitro binding assay of frozen sections (8, 13, 14). Our study also clearly demonstrates that stimulation of T ...
Targeting the dynamic tumor immune microenvironment (TIME) can provide effective therapeutic strategies for cancer. Neutrophils are the predominant leukocyte population in mice and humans, and mounting evidence implicates these cells during tumor growth and metastasis. Neutrophil extracellular traps (NETs) are networks of extracellular neutrophil DNA fibers that are capable of binding tumor cells to support metastatic progression. Here, we demonstrate that circulating NET levels are elevated in advanced esophageal, gastric, and lung cancer patients compared with local cancers and healthy controls. Using preclinical murine models of lung and colon cancer, in combination with intravital video microscopy, we show that NETs functionally regulate disease progression and that blocking NETosis through multiple strategies significantly inhibits spontaneous metastasis to the lung and liver. Furthermore, we show how inhibiting tumor-induced NETs decreases cancer cell adhesion to liver sinusoids following ...
Cytotoxic CD8(+) T cells are considered important effector cells contributing to neuronal damage in inflammatory and degenerative CNS disorders. Using time-lapse video microscopy and two-photon imaging in combination with whole-cell patch-clamp recordings, we here show that major histocompatibility class I (MHC I)-restricted neuronal antigen presentation and T cell receptor specificity determine CD8(+) T-cell locomotion and neuronal damage in culture and hippocampal brain slices. Two separate functional consequences result from a direct cell-cell contact between antigen-presenting neurons and antigen-specific CD8(+) T cells. (1) An immediate impairment of electrical signaling in single neurons and neuronal networks occurs as a result of massive shunting of the membrane capacitance after insertion of channel-forming perforin (and probably activation of other transmembrane conductances), which is paralleled by an increase of intracellular Ca(2+) levels (within ,10 min). (2) Antigen-dependent ...
We apply three-dimensional confocal microscopy and time lapse video-microscopy techniques to visualize molecular dynamics at the immunological synapse (IS). The major contribution of our research team to the field during the last few years has been to contribute to define the biological function of the IS. We propose that the IS has no specific function in T cell activation, on the contrary it is the "manifestation" of the inter-cellular communication occurring during T cell/APC cognate interactions. Our results show that: i) the large-scale molecular clustering and segregation characteristic of a mature IS is not required for productive TCR triggering and for T cell activation (indeed some responses such as cytotoxicity can occur in the absence of mature IS formation); ii) T cells form different types of synapses depending on the strength and quality of antigenic stimulation; iii) synapses can be interrupted and re-formed while T cells add-up the interrupted signals; iv) synapses are not static ...
A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. ...
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To use the proscope with a biological microscope, first remove the lens by gently pressing down on the lens-release button and then rotating and removing the lens. Replace the lens with the threaded ring adapter, and then thread the sleeve into the adapter ring ...
Perineural invasion of cancer cells (CPNI) is found in most patients with pancreatic adenocarcinomas (PDA), prostate, or head and neck cancers. These patients undergo palliative rather than curative treatment due to dissemination of cancer along nerves, well beyond the extent of any local invasion. Although CPNI is a common source of distant tumor spread and a cause of significant morbidity, its exact mechanism is undefined. Immunohistochemical analysis of specimens excised from patients with PDAs showed a significant increase in the number of endoneurial macrophages (EMΦ) that lie around nerves invaded by cancer compared with normal nerves. Video microscopy and time-lapse analysis revealed that EMΦs are recruited by the tumor cells in response to colony-stimulated factor-1 secreted by invading cancer cells. Conditioned medium (CM) of tumor-activated EMΦs (tEMΦ) induced a 5-fold increase in migration of PDA cells compared with controls. Compared with resting EMΦs, tEMΦs secreted higher ...
From annual benefits, like Pittsburghs 50 Finest Gala, to concerts and shows, our Semi-Annual Event Guide is here to fill your calendar through January! Save the dates with Editor in Chief Rachel Jones on todays Dish on WISH 99.7.. ...
A whirl of bone and gore batters nearby enemies every 1 sec, dealing 16,629 Shadow damage every 1 sec, and healing you for 2% of your maximum health every time it deals damage. Lasts 1 sec per 10 Runic Power spent ...
We prepare you physically and psychologically for your upcoming procedure and can help limit some of the negative consequences that can occur during the time lapse between diagnosis and procedure.
in all dimensions. The techniques time resolution is limited only by the frame rate of the video camera. Rayleigh-Sommerfeld back-propagation has the advantage of providing a model-free approach to reconstructing the light field scattered by microscopic objects, and thus lends itself to high-speed processing and imaging. Holographic video microscopy thus can provide real-time feedback for three-dimensional micromanipulation of nanowires and nanorods. It also is useful for studying the rotational and translational motions of nanorods subjected to external forces. The present study takes advantage of the comparative simplicity of single isolated nanorods diffusion when viewed in the co-oriented frame of reference. This advantage is lost when studying the coupled motions of multiple nanorods, so that measurements of nanorods hydrodynamic and electrostatic interactions will be substantially more challenging than corresponding measurements on colloidal spheres. This complexity, however, arises ...
Rachel Finks current research deals with the cytoskeletal dynamics in living fish embryos, with a focus on how microfilament and microtubule behavior relate to cell arrangements during embryogenesis. In addition to her work on fish embryos, Fink also develops research video footage available for teaching and has published two compilations of movies: A Dozen Eggs: Time-Lapse Microscopy of Normal Development (Society for Developmental Biology and Sinauer Associates) and CELLebration (American Society for Cell Biology and Sinauer Associates).. Fink is known for her interactive courses, using everything from colorful chalk to research videos and student presentations to engage students in the material. Of her video footage, Fink notes, "As an embryo develops from a fertilized egg, cells are constantly dividing, migrating, differentiating, and rearranging. Time-lapse video microscopy reveals these movements and allows analysis of the cellular mechanisms driving these transformations." Finks courses ...
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Its a standing joke that creationists demand a complete time-lapse recording of evolution before theyre going to believe it. Joke no more: weve got one. Its a movie of bacteria evolving antibiotic resistance. I dont even need to explain it, because the video explains everything thats going on.
Lixin Liu is the author of this article in the Journal of Visualized Experiments: Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
FLOW 800 is a unique fluorescence application enabling visual analysis of vascular blood flow dynamics. It compiles INFRARED 800 video sequences into visual maps, diagrams or side-by-side images, enabling an in-depth interpretation of fluorescence videos.
Have you seen the video cover from CrystEngComm? Illustrating the work published in their CrystEngComm article, Bergström et al. provide us with an overview of the growth process of self-assembled mesocrystals and ordered arrays from iron oxide nanocubes. By using visible light video microscopy, the authors were able to follow the movement of the growth crystal growth front over several minutes, capturing the transition from symmetrical "coffee-ring" deposition to dendritic or "finger-like" growth. The article forms part of the Nanocrystal growth via oriented attachment themed issue which is available here. In a first for both CrystEngComm and the Royal Society of Chemistry, a short video clip of the crystal growth has been embedded into the journal cover which you can access online here. To view the video, simply click on the main cover image and watch the cover come to life!* Adobe Acrobat 9 or higher is required (http://get.adobe.com/reader/). If the video does not play in your browser, ...
Time Lapse Imaging is a technology also enables frequent monitoring of the progress and development of embryos without exposing them to extraneous elements. This technique covers important parameter like morphokinetic timings, fertilization, compaction etc.
Human neutrophils (white blood cells) are attracted to a chemotactic agent in this time-lapse video. This is one of many videos available for the classroom or broadcast.
Thanks to intravital microscopy, an optical imaging technique, Weigert and colleagues were able to determine for the first time how exocytosis actually occurs in the salivary glands of a living mouse.
Symptom of High Vibration Cause Test or Check Correction subsynchronous a. rubs caused by seals and bearings a. seen as whole fractions (1/2, 1/3) on real time a relieve rub components of running analyzer speed b. oil whirl b. typically 40% to 45% of running speed b. re-design bearing c. oil whip c. a whirl exciting a resonance c. re-design bearing. Get Price → ...
Techniques for generation of novel time-lapse videos that address the inherent sampling issues of traditional photographic time-lapse capture with the use of non-uniform sampling and non-linear filtering ...
Working on observations of bacteria going undercover in ways that might trick the human immune system, Stanford bioengineers have created a time-lapse video that shows this process step by step.
By Dave Andrusko No secret, I am a pushover when it comes to time-lapse videos that "document" a babys development from the very early stages to delivery-and beyond. I watch and re-watch them over and over. For example, just this morning, I revisited a 1:32 video-"A Photo Every Pregnancy Day for 9 Months"-at http://vimeo.com/30039206. There ...
Using filters that only pass light when excited hydrogen atoms change state, pro photographer Cory Poole stacked 700 frames to uncover prominences and other disturbances of the Suns chromosphere. Learn how he did it at corypoole.com.
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Kinetics of compaction on single DNA molecules are studied by fluorescence videomicroscopy in the presence of 1), Xenopus egg extracts and 2), purified nucleosome reconstitution systems using a combination of histones with either the histone chaperone Nucleosome Assembly Protein (NAP-1) or negatively charged macromolecules such as polyglutamic acid and RNA. The comparison shows that the compaction rates can differ by a factor of up to 1000 for the same amount of histones, depending on the system used and on the presence of histone tails, which can be subjected to post-translational modifications. Reactions with purified reconstitution systems follow a slow and sequential mechanism, compatible with the deposition of one (H3-H4)(2) tetramer followed by two (H2A-H2B) dimers. Addition of the histone chaperone NAP-1 increases both the rate of the reaction and the packing ratio of the final product. These stimulatory effects cannot be obtained with polyglutamic acid or RNA, suggesting that yNAP-1 ...
A differential interference contrast (DIC) determination device and method utilizes an illumination source, a layer having a pair of two apertures that receive illumination from the illumination source, and a photodetector to receive Youngs interference from the illumination passing through the pair of two apertures. In addition, a surface wave assisted optofluidic microscope and method utilize an illumination source, a fluid channel having a layer with at least one aperture as a surface, and a photodetector that receives a signal based on the illumination passing through the aperture. The layer is corrugated (e.g., via fabrication) and parameters of the corrugation optimize the signal received on the photodetector.
Poly(ADP-ribosyl)ation of proteins is an important switch in cellular processes including differentiation. In particular, recent advances reveal emerging role in differentiation of cancer cells. Here we described the effect of poly(ADP-ribose) polymerase inhibitor 3-aminobenzamide on a morphological feature of differentiation in transformed endothelial cells GM7373. Exposed to this drug, the cells displayed a 40% growth inhibition due to apoptosis, valued by time-lapse videomicroscopy and TUNEL assay. At the same time, cells showed enhanced motility and, when cultured on Matrigel enriched with FGF2 plus 3-aminobenzamide, began to organize a capillary network, in an Akt phosphorylation dependent manner, accompanied by a cyclooxygenase-2 induction. These results validate the role of poly(ADP-ribose) polymerase activity in cell differentiation and confirm the hypothesis that PARP may be a useful target for anticancer drugs.. ...
The present work investigates dry-friction whip and whirl phenomena for a rigid rotor contacting at two bearing locations. The idea originated with a paper by Clark (2009, "Investigation of the NRG #40 Anemometer Slowdown," American Wind Energy Association, Windpower 2009, Chicago, IL, pp. 1-16) on an anemometer undergoing dry-friction whip and whirl. The anemometer rotor was supported by two Teflon® bushings within an elastically supported housing. The dry-friction forces arose at the bushings. Prior models for dry friction whirl and whip have considered rub at one nonsupport location. The present analytical model consists of a rigid rotor connected to a rigid stator at two rubbing-contact locations. Analytical solutions are developed for the following normal reaction forces at the contact locations: (1) In phase, and (2) 180° out of phase. Analytical solutions are only possible for the same radius-to-clearance ratio (RCl) at the two rub locations and define regions where dry-friction whirl ...
The ability of cancer cells to migrate through a complex three-dimensional (3D) environment is a hallmark event of cancer metastasis. Therefore, an in vitro migration assay to evaluate cancer cell migration in a 3D setting is valuable to examine cancer progression. Here, we describe such a simple migration assay in a 3D collagen-fibronectin gel for observing cell morphology and comparing the migration abilities of cancer cells. We describe below how to prepare the collagen-fibronectin gel castings, how to set up time-lapse recording, how to draw single-cell trajectories from movies and extract key parameters that characterize cell motility, such as cell speed, directionality, mean square displacement, and directional persistence. In our set-up, cells are sandwiched in a single plane between two collagen-fibronectin gels. This trick facilitates the analysis of cell tracks, which are for the most part 2D, at least in the beginning, but in a 3D environment. This protocol has been previously published in
where Vi is field voltage at time point i (ti), and Vi − 1 is field voltage at the previous time point (ti − 1). To correct for underlying baseline "noise," the CBI for an equal duration of recording in the absence of bursts was subtracted from the burst CBI. This sensitive measure of burst intensity is useful for determining the effects of anti-epileptic interventions (Korn et al., 1987; Tallent and Siggins, 1999).. Whole-cell recordings were made from individual granule cells located within the dentate gyrus, identified under infrared differential interference contrast video microscopy with a Carl Zeiss Axioskop 2 FS plus microscope. Patch pipettes were pulled from borosilicate glass (WPI) and filled with the following (in mm): 120 Cs-gluconate, 10 HEPES, 0.1 EGTA, 15 CsCl2, 4 MgCl2, 4 Mg-ATP, and 0.3 Na2-GTP, pH 7.25 (adjusted with 1 m CsOH; 285-290 mOsm; patch electrode resistance, 3-6 MΩ). Electrophysiological data were obtained from granule cells that had a holding current between ...
The Molecular Expressions DIC image gallery contains a wide spectrum of stained and unstained specimens, including transparent living and fixed cells and tissues.
Description • The Zeiss-Biorad Confocal microscope unit features the following capabilities DICM (Differential Interference Contrast Micros...
Look who is hiding in the flower garden? The Snail Whirls has a colorful snail base and 6 removable flower cogs and gears. It helps children to develop fine motor skills and cognitive development. Age range: 18 months +Product size: 9.84 x 8.58 x 1.22Weight: 0.95 lbs
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Most organisms that live in aquatic systems are too small to be seen with the naked eye. To help identify and quantify these organisms, several types of microscopes are utilized. These include flourescence, inverted plankton, dissecting, and video microscopy ...
Instagram has released its new app, Instagram Hyperlapse, a fun and easy way to film and create your own time lapse videos in high quality from your phone.
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