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TY - JOUR. T1 - Application of the principle of optical phase-contrast microscopy to velocity phase-encoded MRI of blood flow in the aorta. AU - Dymond, R C AU - Redpath, Thomas William. AU - McKiddie, F.i.. PY - 1996/5. Y1 - 1996/5. N2 - A new method of presenting magnetic resonance phase information is described and an example of its application given in the context of velocity phase-encoded MRI of blood flow in the aorta. The method takes as its starting point Zernikes technique of phase contrast microscopy. It exploits the parallel between the transform plane in Fourier optics and k-space in MRI. In the example described two datasets are acquired, one with and the other without velocity encoding as in conventional phase-encoded velocity imaging. A dataset is formed which is corrected for unwanted phase variations caused by static field inhomogeneity. The method then effectively combines phase and magnitude information into a single image. The technique is complementary to existing methods ...
Fluorescence Microscope for sale, new Infinitive Plan Phase contrast Microscope Inverted Fluorescence Microscope CE A16.1023 of Opto-Edu (Beijing) Co., Ltd. from China.
Automated visual-tracking systems of stem cell populations in vitro allow for high-throughput analysis of time-lapse phase-contrast microscopy. In these systems, detection of mitosis, or cell division, is critical to tracking performance as mitosis causes branching of the trajectory of a mother cell into the two trajectories of its daughter cells. Recently, one mitosis detection algorithm showed its success in detecting the time and location that two daughter cells first clearly appear as a result of mitosis. This detection result can therefore helps trajectories to correctly bifurcate and the relations between mother and daughter cells to be revealed. In this paper, we demonstrate that the functionality of this recent mitosis detection algorithm significantly improves state-of-the-art cell tracking systems through extensive experiments on 48 C2C12 myoblastic stem cell populations under four different conditions.
In all forms of optical microscopy, the specimen scatters light through processes that include diffraction, refraction, reflection, and absorption. This interactive tutorial explores diffraction of light by a diffraction grating in a phase contrast microscope.
Contents. Preface.. Acknowledgements.. Laboratory Safety.. Microscope Maintenance.. The Micro Kit.. Experiments.. Chapter 1: The Stereomicroscope.. Experiment 1A: Familiarization with the Stereomicroscope.. Chapter 2: The Compound Light Microscope.. Experiment 2A: Familiarization with the Compound Light Microscope. Experiment 2B: Measurements Using the Ocular Micrometer.. Experiment 2C: Microscopic Mounting Techniques.. Experiment 2D: Determining Refractive Index.. Chapter 3: The Polarized Light Microscope.. Experiment 3A: Familiarization with the Polarized Light Microscope.. Experiment 3B: Determining Refractive Index of Anisotropic Materials.. Experiment 3C: Determining Birefringence and Sign of Elongation.. Chapter 4: The Fluorescence Microscope.. Experiment 4A: Familiarization with the Fluorescence Microscope.. Chapter 5: The Phase Contrast Microscope.. Experiment 5A: Familiarization with the Phase Contrast Microscope.. Application Experiments.. Chapter 6: Experiment 6: Physical Match ...
An etoposide-treated DU145 prostate cancer cell exploding into a cascade of apoptotic bodies. The sub images were extracted from a 61-hour time-lapse microscopy video, created using quantitative phase-contrast microscopy. The optical thickness is color-coded. With increasing thickness, color changes from gray to yellow, red, purple and finally black.[1] ...
We have developed diffraction phase microscopy as a new technique for quantitative phase imaging of biological structures. The method combines the principles of common path interferometry and single-shot phase imaging and is characterized by subnanometer path-length stability and millisecond-scale acquisition time. The potential of the technique for quantifying nanoscale motions in live cells is demonstrated by experiments on red blood cells.. © 2006 Optical Society of America. Full Article , PDF Article ...
Quantitative phase retrieval is experimentally demonstrated using the Inverse Compton Scattering X-ray source available at the Accelerator Test Facility (ATF) in the Brookhaven National Laboratory. Phase-contrast images are collected using in-line geometry, with a single X-ray pulse of approximate duration of one picosecond. The projected thickness of homogeneous samples of various polymers is recovered quantitatively from the time-averaged intensity of transmitted X-rays. The data are in good agreement with the expectations showing that ATF Inverse Compton Scattering source is suitable for performing phase-sensitive quantitative X-ray imaging on the picosecond scale. The method shows promise for quantitative imaging of fast dynamic phenomena.. © 2011 Optical Society of America. Full Article , PDF Article ...
An open chambered micro-Slide with 4 wells and an additional intermediate plate | For cell culture and brilliant phase contrast microscopy without meniscus
Meiji Techno offers the MT6800 Series Combo PLM - PCM (Polarized Light and Phase Contrast) Microscopes for Asbestos fiber identification applications. Meiji Techno manufactures these microscopes pursuant to NIOSH 9002 Reference methods and NIOSH 7400 and OSHA ID 160 Reference methods. These models use the phase contrast method for dispersion staining. Each model comes with Strain free Plan DIN brightfield POL objectives, rotatable stage with 360 degree graduations, POL/PHASE/Dispersion Staining Abbe condenser and built-in Koehler illuminator as standard equipment. The table below shows the features of each model.. ...
Methods used by OSHA for the analysis of asbestos (1332214) are described. Equipment at one laboratory is described. Phase contrast microscopes equipped with a polarizer, an analyzer, retardation plates, and rotating stage are explained. X-ray diffraction and transmission electron microscopes with energy dispersive units are also described. Routine determinations of airborne asbestos are made by t
Meiji Techno offers the ML6500 Series PCM (Phase Contrast Microscopes) for Asbestos fiber counting applications. Meiji Techno manufactures these microscopes pursuant to MDHS 39/04 Directive (NIOSH 7400 and OSHA ID 160 Reference methods).. Each model comes with centering telescope, 1mm stage micrometer, GIF 546nm filters and built-in Koehler illuminator as standard equipment. The specifications table below shows the features of each model.. ...
Culturing cells on a slide - posted in Cell Biology: Dear Guys ,In the beginning, I am very grateful for any one who will read my topic. Secondly, I want to buy some culture wares for visualization of my cells under fluorescence or phase contrast microscope.what I found due to reading some books and online: *There are special type of culture slide such as http://www.bdbioscie...y=4&mterms=true *It is untreated.I should treat the surface with Poly L lysine or collagenase as I am working...
... : In situ trypan blue staining of monolayer MCF-7 cell cultures before (a) and after (b) cell treatment with trypsin. Dye stained dead cells. Images were made by using inverted phase contrast microscope (scale bar: 50 m ...
This laboratory hosts all the equipment connected to medium and high magnification optical microscopy with digital cameras for snapshots. Devices at this laboratory allow the observation of samples from particular organisms or tissues that may require sophisticated instrumental techniques with epifluorescence (bacteria and other dyed tissues) or phase-contrast microscopy (small transparent organisms). All the equipment necessary for digital micro-photography snapshots is also available for different purposes (measurements, scientific papers illustration, etc.).. ...
SHALLSONS, LLC has a Nikon Diaphot 200 Inverted phase contrast microscope for sale. The unit is in excellent condition, with few signs of wear and is
Establishment of Hepatocyte Cultures. Cryopreserved hepatocytes were thawed in hepatocyte thawing medium and counted to determine yield. Viability was measured using trypan blue exclusion; only hepatocyte batches with ≥85% viability were used in this study. Isolated hepatocytes were transferred to collagen I-precoated 24-well plates, each well containing a cell density of 0.3 × 106 viable cells in 0.5 ml of hepatocyte plating medium. After 24 h, hepatocyte plating medium was removed and replaced with incubation medium (Williams E + Glutamax, 10% FBS, 1% penicillin/streptomycin, 7 μM insulin, and 1 μM dexamethasone). After an additional 24 h of culturing, the confluence of the hepatocytes was visually assessed using phase contrast microscopy. At the time of dosing, hepatocytes were ∼90% confluent. Following the initial 2-day recovery period, the incubation medium was removed and the hepatocytes were treated daily with induction medium (Williams E + Glutamax, 1% ITS+ premix solution, and ...
The Golgi apparatus is a relatively large, membrane-bound organelle and thus one of the easiest cell structures to study in detail [2]. The organelle is located nearby the cell nucleus and is closely associated with the endoplasmic reticulum. By observing via metallic impregnation, it can be seen through phase contrast microscopy that the Golgi has a convoluted, dense and "ill-formed" morphology [3]. Initial studies have shown that the organelle has great variance in its form dependent on the type of cell it is in as well as the state of activity that the cell is in. There are roughly around 40-100 Golgi apparatus stacks within a mammalian cell [4]. Overall, the Golgi apparatus is made of 4-8 flattened, membrane-bound sacs that are stacked upon one another [5]. These are known as cisternae. The Golgi also includes associated nearby vesicles. Each cisterna primarily contains products from the endoplasmic reticulum, which enter the Golgi at the cis face - the end that is closest to the ER and ...
The Golgi apparatus is a relatively large organelle and thus one of the easiest cell structures to study in detail [3]. The organelle is located nearby the cell nucleus and is closely associated with the endoplasmic reticulum. By observing via metallic impregnation, it can be seen through phase contrast microscopy that the Golgi has a convoluted, dense and "ill-formed" morphology [4]. Initial studies have shown that the organelle has great variance in its form dependent on the type of cell it is in as well as the state of activity that the cell is in. There are roughly around 40-100 Golgi apparatus stacks within a mammalian cell [5]. Overall, the Golgi apparatus is made of 4-8 flattened, membrane-bound sacs that are stacked upon one another [6]. These are known as cisternae. The Golgi also includes associated nearby vesicles. Each cisterna primarily contains products from the endoplasmic reticulum, which enter the Golgi at the cis face - the end that is closest to the ER and accepts incoming ...
The Biotechnology Program of Manhattan College and the College of Mt. St. Vincent is sponsoring: A SHORT COURSE IN BASIC TISSUE CULTURE JUNE 4-8, 1990 9:00 AM-5:00 PM This short course in the culturing of mammalian cells is presented by means of hands-on procedures and demonstrations. All the essential apsects and contemporary methods of growing cells in culture will be covered. There will be lectures on the cultured cell, monolayer and suspension culture systems, contamination, contemporary methods of cell identification, and production of monoclonal antibodies. Hands-on procedures will include pipetting, media preparation and filtration, basic phase contrast microscopy, the hemocytometer method of cell counting, cryogenic preservation, culturing anchorage dependent cells, primary cell culturing, cell identification using immunohistofluorescence, karyotyping, and mycoplasma detection. Demonstrations on the use of cryogenic equipment, various types of culture vessels, DNA characterization, two ...
Bacterial cell attachment to the surfaces of self-assembled monolayers formed by the adsorption of omega-substituted alkanethiols on transparent gold films has been studied under defined bacterial culture and flow conditions. Phase contrast microscopy was used to quantify the attachment of two organ …
Scale bar: 100 μm. B. The proliferation of atypical tumor cells with osteoid formation is shown. Xenografted tumor cells resemble original tumor cells. Scale bar: 50 μm. Cell growth and morphological findings in vitro UTOS-1 cells were spindle-shaped, contained several nucleoli, and formed clumps. Two weeks after initial cultivation in primary culture, the tumor cells reached subconfluence with some Pictilisib piled-up foci selleck products of cells (Figure 4A). After the cells were serially subcultured for about 3 months, they began to grow rapidly at passage 6 (Figure 4B). Figure 4 Morphology under phase-contrast microscopy. A. In primary. culture, spindle-shaped tumor cells reach subconfluence with some piled-up foci of cells. Scale bar: 100 μm. B. At passage. 6, the tumor cells begin to grow rapidly. The configuration of tumor cells is equalized after the 6th generation. Scale bar: 100 μm. This new cell line has been maintained in vitro for more than 50 passages over more than 2 years. ...
In demyelinating or non-demyelinating neurodegenerative diseases, increased levels of 7-ketocholesterol (7KC), 7β-hydroxycholesterol (7β-OHC) and 24(S)-hydroxycholesterol (24S-OHC) can be observed in brain lesions. In 158N murine oligodendrocytes, 7KC triggers a complex mode of cell death defined as oxiapoptophagy, involving simultaneous oxidative stress, apoptosis and autophagy. In these cells, 7KC as well as 7β-OHC and 24S-OHC induce a decrease of cell proliferation evaluated by phase contrast microscopy, an alteration of mitochondrial activity quantified with the MTT test, an overproduction of reactive oxygen species revealed by staining with dihydroethidium and dihydrorhodamine 123, caspase-3 activation, PARP degradation, reduced expression of Bcl-2, and condensation and/or fragmentation of the nuclei which are typical criteria of oxidative stress and apoptosis ...
Time lapse movie of mouse embryonic fibroblasts in culture imaged at 30 second intervals by phase contrast microscopy. A micropipette is positioned n...
There are some key photo-devices in near future, such as solar cell, photocatalyst, and artificial photosynthesis, where photo-excited carriers (electrons and holes) are extracted for specific purpuses. In these devices, assembly of semiconductor particles are used as electrodes. However, most of the carriers are usually trapped to defects, and their lifetime and spatial distribution are diversified, which have not been understood well. In this study, we will develop a new method which can clarify the correlation between the arrangement of particles and the carrier properties, especially on the lifetime and the transport property.. ...
The |i|Journal of Biomedical Optics|/i| (JBO) publishes peer-reviewed papers on the use of novel optical systems and techniques for improved health care and biomedical research.
Phase Contrast Trinocular biological compound 40x-1600x microscope with 2MP USB Camera from MicroscopeNet, Model#: XM837PHB1C20C, quality product for labs, clinics Great selections of Compound, Stereo, Industrial Microscopes
BioTek Anwendungshinweise, 04-Nov-14, Automated Hemocytometer-Based Live/Dead Cell Counting using Phase Contrast and Color Brightfield Imaging
Fluxmedia is a research-creation network exploring the intersections of art, science and technology. The conceptual focus of Fluxmedia considers how the production and manipulation of wet-ware, life-forms and electronic media instigate cultural, philosophical, aesthetic and ethical concerns in art and culture. Fluxmedia currently supports the development and creation of wet lab work with tissue culture and digital imaging with an inverted phase microscope and HD video in its new home at the Speculative Life Laboratory at the Milieux Institute for Arts, Culture and Technology at Concordia University. Fluxmedia is a member of Milieux Institute and Hexagram. Graduate students interested in becoming involved with Fluxmedia are welcome to contact me ...
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer ...
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Refractive index (RI) and its dispersion play a major role in interaction of electromagnetic wave with matter. Quantitative phase imaging (QPI) has proven to be a useful tool to estimate the RI from the sample-induced phase delay measurement at high spatio-temporal resolution. Here, we introduce near-infrared dispersive quantitative phase imaging (NIRD-QPI) of microscopic objects. The setup uses a new geometry for quantitative phase microscopy by use of spatial frequency filtering in Fourier plane. High resolution refractive index spectroscopic measurement over a range from 690 to 840nm in interval of 25nm is reported. This method could prove to be very useful for characterizing wide range of nano and biomaterials ...
Even the cleanest mouth is teeming with billions of microbes, some of which support good oral-systemic health, others which are harmful. By looking at a sample of your oral flora through a phase contrast microscope, we can get quick and clear insight as to whether conditions are healthy overall or there are signs of emerging or progressing gum disease.. If bad bacteria outweigh the good, we can sharpen our understanding with advanced periodontal evaluation while also moving forward with treatment to control the disease process.. In this video, a holistic dentist in Colorado explains how phase contrast microscopy is used:. ...
A differential interference contrast (DIC) determination device and method utilizes an illumination source, a layer having a pair of two apertures that receive illumination from the illumination source, and a photodetector to receive Youngs interference from the illumination passing through the pair of two apertures. In addition, a surface wave assisted optofluidic microscope and method utilize an illumination source, a fluid channel having a layer with at least one aperture as a surface, and a photodetector that receives a signal based on the illumination passing through the aperture. The layer is corrugated (e.g., via fabrication) and parameters of the corrugation optimize the signal received on the photodetector.
Abstract: Fiber counting of asbestos using light microscopy has traditionally been performed using phase contrast microscopy (PCM) techniques. The use of phase contrast for counting has several weaknesses, including difficulties with fiber detection and overlapping diffraction patterns causing the appearance of "false" fibers or masking fibers. It is, however, a significant improvement over ordinary brightfield microscopy. The purpose of this study was to assess the use of two other contrast techniques, Hoffman modulation contrast (HMC) and Nomarski differential interference contrast (DIC). Samples of three fiber types, chrysotile, amosite, and man-made mineral fibers (MMMF) were used for this evaluation. DIC faired poorly in general and HMC faired reasonably well. The differences can be explained by the general aspects of contrast production in reference to phase detection and imaging of each method. Full article (PDF) ...
A bright field microscope can be used for evaluating motility if the field diaphragm is closed to enhance contrast and ability to visualize sperm. A much better choice is a phase contrast microscope or a microscope equipped for differential interference contrast (DIC). Examine the images above to appreciate the difference in these three types of microscopy - clearly, unstained sperm are difficult to observe using bright field. Manual motility estimates are easy to perform and require minimal equipment. In the hands of an experienced evaluator, manual estimates generally provide good estimates of motility. The chief limitation of this technique is its subjective nature. Samples with outstanding motility tend to be scored lower than they should be ("it cant be that good ..") and poor samples tend to be scored higher than they should ("it cant be that bad .."). Track motility estimates: A wet mount of diluted semen is prepared as described for a manual estimate, and sperm are photographed using ...
Reliable autofocus is required to obtain accurate measurements of fluorescent stained cellular components from a system capable of scanning multiple microscope fields. Autofocus could be performed directly with fluorescence images, but due to photobleaching and destructive fluorescence by-products, it is best to minimize fluorescence exposure for photosensitive specimens and live cells. This exposure problem could be completely avoided by using phase-contrast microscopy, implemented through the same optics as fluorescence microscopy. Functions for both phase-contrast and fluorescence autofocus were evaluated using the present invention and the suitability of phase-contrast autofocus for fluorescence microscopy was determined. The present autofocus system for scanning microscopy can be performed at least as fast as 0.25 s/field without loss of precision. The speed of autofocus can be further increased by a volume image which is obtained by observing an image object at each image plane of a plurality of
Objectives: To develop pooled size-specific asbestos fiber exposure estimates for North Carolina and South Carolina asbestos textile plants. Methods: Airborne sample data and prior exposure estimates by phase-contrast microscopy (PCM) for the two cohorts were reviewed and compared. Estimates by transmission electron microscopy (TEM) for 160 membrane filter samples from all plant were pooled. Poiss
Transwell permeable supports are available in three membrane materials: polycarbonate, polyester (PET), and collagen-coated polytetrafluoroethylene (PTFE). Polycarbonate Transwell inserts feature thin, translucent membranes available in four pore sizes ranging from 0.4 µm to 8.0 µm. Most are treated for optimal cell attachment. They are supplied sterile and come preloaded in multiple well plates or dishes. The polycarbonate membrane is compatible with most organic fixatives and stains. Polyester (PET) Transwell-Clear inserts have microscopically transparent polyester membranes that are tissue culture-treated for optimal cell attachment and growth. Transwell-Clear inserts provide better cell visibility under phase contrast microscopy and allow assessment of cell viability and monolayer formation. Transwell-COL inserts have transparent (when wet), collagen-treated PTFE membranes that promote cell attachment and spreading and allow cells to be visualized during culture. The Transwell-COL ...
Long fixation and staining steps are not necessary anymore.. The Trumorph is based upon examination of wet preparations. of living immobilized spermatozoa, after a short 60°C incubation,. in narrow chambers and examined by negative phase contrast microscopy.. The Trumorph makes it possible to analyze spermatozoa from. the head to the tail and to detect morphological abnormalities.. The ease of preparation makes it a robust method applicable for analysis. of living unmodified spermatozoa.. ...
If the aphorism "one picture worth a thousand words . . .," attributed to Confucius, 551-478 B.C., is correct, the authors of this paperback book have succeeded in reducing the length of the text by more than one third. Illustrative material forms the basis of this book and is mainly a result of excellent use of the electron microscope. These illustrations consist of scanning electron micrographs and detailed diagrams, supplemented with a few well-placed transmission micrographs and photomicrographs obtained by light-and phase-contrast microscopy. Although most of the illustrations are excellent in quality, the text describing, explaining, and expanding on what ...
The control and mechanisms of airway smooth muscle cell (SMC) contraction were investigated with a sequential series of lung slices from different generations of the same airway from the cardiac lobe of the mouse lung. Airway contraction was measured by monitoring the changes in airway lumen area with phase-contrast microscopy. Changes in intracellular calcium concentration of the SMCs were studied with a custom-built confocal or two-photon microscope. The distribution of the airway SMCs and the muscarinic M(3) or 5-HT(2A) receptors was determined with immunofluorescence. Methacholine and 5-HT induced a concentration-dependent airway contraction and Ca(2+) oscillations within the SMCs of each airway generation. The airway contraction in response to the same agonist concentration was greater in the middle generation compared with the distal or proximal generations of the same airway. Similarly, the Ca(2+) oscillations varied in different generations of the same airway, with a slower frequency in the SMCs
A cell disrupter has been developed which can measure the forces required to disrupt both eukaryotic and prokaryotic cells. It operates a continuous process and will disrupt both large and small volumes. Shear forces are set up when a suspension under laminar flow conditions is released under high pressure through a short orifice. If the applied pressure is altered, the shear forces are simultaneously changed so that the amount of cell disruption can be compared under different known and repeatable conditions. The disrupter is now manufactured and supplied by Stansted Fluid Power Limited, Stansted, England. Phase-contrast microscopy has shown that the disrupter will break a variety of organisms including Chlorella, Aspergillus fumigatis, Fusarium sp., Saccharomyces cerevisiae, Escherichia coli, Lactobacillus casei, Bacillus subtilis, Clostridium perfringens, Streptococcus faecalis, Streptococcus zooepidermicus and Staphylococcus aureus. The cells are not all broken at one pressure but a certain ...
UCH-L1 supports cell survival in H838 cells Assessment of H838 and H157 cells exhibiting reduced UCH-L1 protein levels by phase-contrast microscopy revealed morphological changes in the UCH-L1 siRNA-treated H838 cells compared to scrambled siRNA- treated and untreated control cells, whereas no difference was observed between UCH-L1 siRNA-treated H157 cells. and control H157 cells. Normally the parental H838 cells were rounded in shape and uniform in size, but cells with reduced UCH-L1 expression were irregular in shape, PF 2341066 variable in size, and present at a much lower density. H838 cells with low levels of UCH-L1 were also less flattened to the surface, possibly signifying they were becoming detached, a characteristic of apoptotic cells (Figure 4A). Therefore untreated and treated selleck products H838 cells were stained with H&E to compare the number of apoptotic cells. Definite apoptotic changes were observed in the UCH-L1 siRNA-treated cells (Figure 4B). To quantify the differences in ...
The Multifunctional Staring Mode Microscope was developed to permit three modes of imaging for cell counting in mouse embryos: Optical Quadrature, Differential Interference Contrast (DIC), and Fluorescence Imaging. The Optical Quadrature Microscope, consisting of a modified Mach-Zender Interferometer, uses a 632.8 nm laser to measure the amplitude and phase of the signal beam that travels through the embryo. Four cameras, preceded by multiple beamsplitters, are used to read the four interferograms, which are then combined to produce an image of the complex electric field amplitude. The phase of the complex amplitude is then unwrapped using a 2-D phase unwrap algorithm and images of optical path length are produced. To combine the additional modes of DIC and Fluorescence Imaging with the Optical Quadrature Microscope, a 632.8 nm narrow bandpass beamsplitter was placed at the output of the microscope. This allows the laser light to continue through the Mach-Zender while all other wavelengths are ...
Responses of cultured mouse fibroblasts, human erythrocytes, bovine erythrocytes, and liposomes with different phosphatidylcholine (PC) and sphingomyelin (SPM) ratios to both natural and synthetic lytic peptides were characterized. Peptide-induced morphological alterations of the plasma membrane of cells were examined by various light microscopic techniques and scanning electron microscopy (SEM). The ability of natural and synthetic peptides to kill cells and cause increased permeability of liposomes was evaluated using Trypan Blue (TB) dye exclusion assay and fluorescent dye leakage assay, respectively. Differential interference contrast microscopy, SEM, and fluorescence studies revealed characteristic structural and lipid changes in the plasma membrane of lytic peptide-treated fibroblasts, and these alterations were accompanied by simultaneous changes in the cell permeability as indicated by the uptake of TB. Formation of membrane vesicles, composed primarily of lipids, was demonstrated in cells
Cell-cell adhesion is at the top of a molecular cascade of protein interactions that leads to the remodeling of epithelial cell structure and function. The earliest events that initiate this cascade are poorly understood. Using high resolution differential interference contrast microscopy and retrospective immunohistochemistry, we observed that cell-cell contact in MDCK epithelial cells consists of distinct stages that correlate with specific changes in the interaction of E-cadherin with the cytoskeleton. We show that formation of a stable contact is preceded by numerous, transient contacts. During this time and immediately following formation of a stable contact, there are no detectable changes in the distribution, relative amount, or Triton X-100 insolubility of E-cadherin at the contact. After a lag period of approximately 10 min, there is a rapid acquisition of Triton X-100 insolubility of E-cadherin localized to the stable contact. Significantly, the total amount of E-cadherin at the ...
A human malignant continuous cell line, named NG97, was recently established in our laboratory. This cell line has been serially subcultured over 100 times in standard culture media presenting no sign of cell senescence. The NG97 cell line has a doubling time of about 24 h. Immunocytochemical analysis of glial markers demonstrated that cells are positive for glial fibrillary acidic protein (GFAP) and S-100 protein, and negative for vimentin. Under phase-contrast microscope, cultures of NG97 showed cells with variable morphological features, such as small rounded cells, fusiform cells (fibroblastic-like cells), and dendritic-like cells. However, at confluence just small rounded and fusiform cells can be observed. At scanning electron microscopy (SEM) small rounded cells showed heterogeneous microextentions, including blebs and filopodia. Dendritic-like cells were flat and presented extensive prolongations, making several contacts with small rounded cells, while fusiform cells presented their surfaces