This thesis considers the possible mechanisms of haematuria in cases of thin basement membrane disease and mild forms of IgA nephropathy. The absence of inflammation in both diseases makes it difficult to explain the passage of erythrocytes to the urinary space.;Two main approaches were used. The composition of glomerular basement membrane was evaluated using quantitative immuno-electron microscopy; this was successfully achieved with antibodies against collagen IV, laminin, fibronectin and collagen VI. Possible changes in degradation by proteolytic enzymes were assessed using quantitative in situ zymography, a novel technique where the ability of tissue sections to digest gelatin in a layer of photographic emulsion is measured.;Initial experiments were performed to establish the techniques and to assess sources of variation in the final measurements. Reproducibility studies for immuno-electron microscopy and in situ zymography demonstrated a considerable amount of variation if processing of ...
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The dentate gyrus has been shown to receive a laminated and target selective GABAergic input (Han et al., 1993; Halasy and Somogyi, 1993), but the numerical parameters of this innervation are not known. In order to establish the relative weight of GABAergic inputs to the dendritic versus somatic regions of granule cells the numerical density and proportion of GABA-immunopositive and immunonegative synaptic boutons and their postsynaptic targets were determined in the molecular and granule cell layers of the dentate gyrus using the postembedding immunogold method. The granule cell layer contained 9% of all synapses with the remaining 91% being in the molecular layer. Altogether 17% of all synaptic boutons were GABA-immunoreactive, and they formed either type 1 or type 2 synaptic junctions. About 88% of synaptic boutons in the granule cell layer and 7-8% in the molecular layer were GABA-positive. However, the numerical density (number of synapses per unit volume) of GABA-immunoreactive type 2 ...
Immunogold EM was employed to compare the distribution of type 1 plasminogen activator inhibitor (PAI-1) on the surface of agonist-activated human umbilical vein endothelial cells (HUVECs) with that of control, unactivated cells. As previously observed, (Schleef, R.R., T.J. Podor, E. Dunne, J. Mimuro, and D.J. Loskutoff. J. Cell Biol. 110:155-163), analysis of cross-sections of nonpermeabilized control HUVEC monolayers stained first with affinity-purified rabbit antibodies to PAI-1 and then with gold-conjugated goat anti-rabbit IgG, revealed the presence of relatively few gold particles (less than 1-2% of the total) on the apical cell surface. The majority of gold particles were detected primarily in the extracellular matrix between the culture substratum and the cell membrane. In contrast, treatment of HUVECs with tumor necrosis factor alpha (TNF alpha; 200 U/ml, 24 h) or with lipopolysaccharide (LPS; 10 micrograms/ml, 24 h) resulted in an increased staining of PAI-1 not only in the ...
While several methods are available for characterizing ligand localization (e. g. labeling with radioactive or electrondense tracers), receptor molecules themselves can only be localized in situ by...
Kinetoplasmid membrane protein-11 (KMP11) is present in a wide range of trypanosomatids. In the present paper, we show that the T. cruzi KMP11 gene is organized in a cluster formed by four gene units arranged in a head-to-tail tandem manner located on a chromosome of about 1900 kb. Northern blot analyses indicated that the steady-state level of mature KMP11 transcripts of 0.52 kb is high and similar in the three forms of the parasite. The KMP11 mRNAs have a half-life of about 16 h whose steady-state level is strongly downregulated when the parasites reach the stationary growth phase. The T. cruzi KMP11 sequence presents a significant homology with the amino-terminal third of the cytoskeleton-associated protein CIP1 from Arabidopsis thaliana. Western blot and immunoelectron microscopy studies showed that KMP11 is present in the cytoskeleton structure. Because the strong downregulation observed in the de novo synthesis of KMP11 protein in parasites treated with vinblastine is not accompanied by a ...
Specific molecular forms of GST are known to be expressed in preneoplastic cells, and have been known to participate in their resistance to drugs. GST-pi are present in most epithelial tissues of the human gastrointestinal tract. Significant amounts of the class pi GST was expressed in the majority of human tumors and human tumor cell lines. Studies have shown that GST-pi expressed highly in neoplasm and could be regarded as a tumor marker. However the immunohistochemical and the immunoelectron microscopical localization of GST-pi, in human polyps, are not clear. ...
The membrane-spanning and cytoplasmic domains of CD4 and CD8 were replaced by those of TGN38. After transient expression in HeLa cells, the location of the hybrid proteins was determined using immunofluorescence and quantitative immuno-electron microscopy, FACS analysis and metabolic labeling. The membrane-spanning domain was found to contain a signal that localized hybrid proteins to the TGN. This was in addition to the signal previously identified in the cytoplasmic domain (Bos, K., C. Wraight, and K. Stanley. 1993. EMBO (Eur. Mol. Biol. Organ) J. 12:2219-2228. Humphrey, J. S., P. J. Peters, L. C. Yuan, and J. S. Bonifacino. 1993. J. Cell Biol. 120:1123-1135. Wong, S. H., and W. Hong. 1993. J. Biol. Chem. 268:22853-22862). The different properties of these two signals suggest that each operates by a different mechanism. ...
Noradrenergic innervation of rat liver was studied immunohistochemically using antibody to noradrenaline at the electron-microscopic level. Noradrenaline-immunoreactive nerve fibres were located in the portal tract and some were in close contact with
Page contains details about SiO2-PEG4-24-Tf bionanoparticles labeled with immunogold . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Immunoelectron microscopy identifying avidin (8-nm gold) and cathepsin L (15-nm gold) immunoreactivity in organelles from late endosome, lysosome, and hybrid
Definition: applying immunohistochemistry or immunocytochemistry method to localize antigens or proteins at sub-cellular level using an electron microscope.. General Methods & Techniques. Antigen Retrieval Methods. Multiple Labeling Methods. Antigen-Antibody Specific Applications. Tissue-Cell Specific Applications. Virological Applications. Tumor, Disease & Diagnostic Applications. TEM Immunogold Labeling Protocol - Pre-embedding Method (IHC World) - Detailed step by step procedure for EM immunogold staining technique on ultrathin sections.. TEM Immunogold Labeling Protocol - Post-embedding Method-1 (IHC World) - Detailed step by step procedure for EM immunogold staining technique on ultrathin sections.. TEM Immunogold Labeling Protocol - Post-embedding Method-2 (IHC World) - Detailed step by step procedure for EM immunogold staining technique on ultrathin sections.. ...
Aquaporin-4 (AQP4) is the predominant water channel in brain and is selectively expressed in astrocytes. Astrocytic endfoot membranes exhibit tenfold higher densities of AQP4 than non-endfoot membranes, making AQP4 an excellent marker of astrocyte polarization. Loss of astrocyte polarization is known to compromise astrocytic function and to be associated with impaired water and K+ homeostasis. Here we investigate by a combination of light and electron microscopic immunocytochemistry whether amyloid deposition is associated with a loss of astrocyte polarization, using AQP4 as a marker. We used the tg-ArcSwe mouse model of Alzheimers disease, as this model displays perivascular plaques as well as plaques confined to the neuropil. 3D reconstructions were done to establish the spatial relation between plaques and astrocytic endfeet, the latter known to contain the perivascular pool of AQP4. Changes in AQP4 expression emerge just after the appearance of the first plaques. Typically, there is a loss ...
The Escherichia coli 23 S and 5 S rRNA molecules have been fitted helix by helix to a cryo-electron microscopic (EM) reconstruction of the 50 S ribosomal subunit, using an unfiltered version of the recently published 50 S reconstruction at 7.5 A resolution. At this resolution, the EM density shows a well-defined network of fine structural elements, in which the major and minor grooves of the rRNA helices can be discerned at many locations. The 3D folding of the rRNA molecules within this EM density is constrained by their well-established secondary structures, and further constraints are provided by intra and inter-rRNA crosslinking data, as well as by tertiary interactions and pseudoknots. RNA-protein cross-link and foot-print sites on the 23 S and 5 S rRNA were used to position the rRNA elements concerned in relation to the known arrangement of the ribosomal proteins as determined by immuno-electron microscopy. The published X-ray or NMR structures of seven 50 S ribosomal proteins or ...
Based on its early recruitment to the satellite SPB and its requirement for SPB localization of multiple SPB components, we propose that Ppc89 functions as a platform for assembly of a new SPB (Fig. 7 A). The C terminus of Ppc89 has previously been shown to interact with Sid4 (Rosenberg et al., 2006), and our SPA-SIM data show that this end of Ppc89 extends away from the NE to function in assembly of an outer module that includes Sid4, Cdc11, and Mto1. The N terminus of Ppc89 is located near the C terminus of Pcp1, which would facilitate assembly of a central module, which contains Cam1 in addition to Pcp1 (Fig. 7 B). The idea that Pcp89 connects SPB submodules makes it functionally analogous to Spc42 in budding yeast. Like Ppc89, Spc42 is also the first component recruited to the bridge, and its C and N terminus interact with orthologues of Sid4 (Cnm67) and Pcp1 (Spc110; Muller et al., 2005; Burns et al., 2015). Although SPA-SIM and immuno-EM data suggest that fluorophores on the N terminus of ...
Application: To determine the ultrastructure of tissues and cells at 2nm resolution by transmission electron microscopy (TEM).. Method: Tissues or cells are chemically fixed, dehydrated, embedded in resin and stained. Thin slices are cut from the resin block using an ultramicrotome and imaged at room temperature by TEM. Immuno-EM is possible before the embedding or after the sectioning steps.. ...
Distribution of TRP1 in the Golgi and associated vesicles. (A) Ultrathin cryosections of MNT-1 cells were labeled with anti-TRP1 and PAG 5. TRP1 is often enrich
Abstract: We have developed a new standardized method for the post-embedding immunoelectron microscopy using the same fixation, antigen retrieval and image contrasting procedures. Tissues were fixed with 4% formaldehyde containing 2.5 mM CaCl2, 1.25 mM MgCl2 in a 0.1 M 4-(2-hydroxyethyl)-piperazineethanesulfonic acid (HEPES) buffer (pH 7.4) for 2 h and then with the same fixative composition in 0.1 M HEPES buffer (pH 8.5) overnight at room temperature. Vehicle osmolarity of fixatives was adjusted to 300-330 mOsm by adding glucose. The specimens were dehydrated with dimethylformamide on ice and embedded in LR-White resin. Ultrathin sections were heated in a 20 mM Tris-HCl buffer (pH 9.0) for 1-2 h at 95°C. After immuno-gold labeling, the sections were treated with 2% glutaraldehyde containing 0.05% tannic acid in a 0.1 M phosphate buffer (pH 5.5) for 5 min and with a 1% OsO4/0.1 M phosphate buffer (pH 7.4) for 5 min, and then they were double stained with uranyl acetate and lead citrate. The ...
TY - JOUR. T1 - Glycoproteins of coated pits, cell junctions, and the entire cell surface revealed by monoclonal antibodies and immunoelectron microscopy.. AU - Murphy, T. L.. AU - Decker, G.. AU - August, Thomas. PY - 1983/8. Y1 - 1983/8. N2 - Topographical descriptions of three major plasma membrane glycoproteins of murine 3T3 cells were obtained by immunoelectron microscopy with monoclonal antibodies. A glycoprotein of Mr 80,000 was distributed throughout the total cell surface. A second of Mr 90,000 was concentrated in coated pits, and a third of Mr 100,000 was localized at cell junctions.. AB - Topographical descriptions of three major plasma membrane glycoproteins of murine 3T3 cells were obtained by immunoelectron microscopy with monoclonal antibodies. A glycoprotein of Mr 80,000 was distributed throughout the total cell surface. A second of Mr 90,000 was concentrated in coated pits, and a third of Mr 100,000 was localized at cell junctions.. UR - ...
We generated transgenic (Thy1alpha6) mice in which the GABA(A) receptor alpha6 subunit, whose expression is usually confined to granule cells of cerebellum and cochlear nuclei, is ectopically expressed under the control of the pan-neuronal Thy-1.2 promoter. Strong Thy1alpha6 subunit expression occurs, for example, in deep cerebellar nuclei, layer V iscocortical and hippocampal pyramidal cells and dentate granule cells. Ligand binding and protein biochemistry show that most forebrain alpha6 subunits assemble as alpha6betagamma2-type receptors, and some as alpha1alpha6betagamma2 and alpha3alpha6betagamma2 receptors. Electron microscopic immunogold labeling shows that most Thy1-derived alpha6 immunoreactivity is in the extrasynaptic plasma membrane of dendrites and spines in both layer V isocortical and CA1pyramidal cells. Synaptic immunolabeling is rare. Consistent with the alpha6 subunits extrasynaptic localization, Thy1alpha6 CA1 pyramidal neurons have a five-fold increased tonic GABA(A) receptor
Pericytes, perivascular cells embedded in the microvascular wall, are crucial for vascular homeostasis. These cells also play diverse roles in tissue development and regeneration as multi-lineage progenitors, immunomodulatory cells and as sources of trophic factors. Here, we establish that pericytes are renin producing cells in the human kidney. Renin was localized by immunohistochemistry in CD146 and NG2 expressing pericytes, surrounding juxtaglomerular and afferent arterioles. Similar to pericytes from other organs, CD146(+)CD34(-)CD45(-)CD56(-) renal fetal pericytes, sorted by flow cytometry, exhibited tri-lineage mesodermal differentiation potential in vitro. Additionally, renin expression was triggered in cultured kidney pericytes by cyclic AMP as confirmed by immuno-electron microscopy, and secretion of enzymatically functional renin, capable of generating angiotensin I. Pericytes derived from second trimester human placenta also expressed renin in an inducible fashion although the renin activity
Results The LVP consists of a broad nucleocapsid surrounding an electron-dense centre, presumably containing the HCV genome. The nucleocapsid is surrounded by an irregular, detergent-sensitive crescent probably composed of lipids. Lipid content may determine particle size. These particles carry HCV E1E2, ApoB and ApoE, as shown in our immuno-EM analysis. Our results also suggest that these putative LVPs circulate in the serum of patients as part of a mixed population, including lipoprotein-like particles and complete viral particles. ...
Results The LVP consists of a broad nucleocapsid surrounding an electron-dense centre, presumably containing the HCV genome. The nucleocapsid is surrounded by an irregular, detergent-sensitive crescent probably composed of lipids. Lipid content may determine particle size. These particles carry HCV E1E2, ApoB and ApoE, as shown in our immuno-EM analysis. Our results also suggest that these putative LVPs circulate in the serum of patients as part of a mixed population, including lipoprotein-like particles and complete viral particles. ...
Researchers at the MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, The Netherlands, are developing a method of detecting and treating tumors with the help of gold particles with dimensions ...
(PhysOrg.com) -- It has long been known that heat is an effective weapon against tumor cells. However, its difficult to heat patients tumors without damaging nearby tissues.
TY - JOUR. T1 - Immunoelectron microscopic demonstration of insulin-stimulated translocation of glucose transporters to the plasma membrane of isolated rat adipocytes and masking of the carboxyl-terminal epitope of intracellular GLUT4. AU - Smith, Robert M.. AU - Charron, Maureen J.. AU - Shah, Neelima. AU - Lodish, Harvey F.. AU - Jarett, Leonard. PY - 1991/8/1. Y1 - 1991/8/1. N2 - Polyclonal antibodies to the amino- or carboxyl-terminal peptide sequences of the GLUT4 transporter protein were used in immunoelectron microscopic studies to demonstrate the location and insulin-induced translocation of GLUT4 in intact isolated rat adipocytes. Labeling of untreated adipocytes with the amino-terminal antibody revealed 95% of GLUT4 was intracellular, associated with plasma membrane invaginations or vesicles contiguous with or within 75 nm of the cell membrane. Insulin treatment increased plasma membrane labeling ≈13-fold, to 52% of the total transporters, and decreased intracellular labeling ...
Electron microscopic localization of acetylcholinesterase in the superior cervical ganglion of the rat. In: Kolligátum. pp. 274-285. (1967 ...
in European Journal of Cell Biology (1988), 47(2), 346-57. In order to investigate the DNA localization within Ehrlich tumor cell nucleoli during mitosis, two recent immunocytochemical methods using either an anti-DNA or an anti-bromodeoxyuridine (BrdU ... [more ▼]. In order to investigate the DNA localization within Ehrlich tumor cell nucleoli during mitosis, two recent immunocytochemical methods using either an anti-DNA or an anti-bromodeoxyuridine (BrdU) monoclonal antibody have been applied. In both cases, the immunogold labeling has been performed on ultrathin sections of cells embedded either in Lowicryl K4M or in Epon, respectively. Identical results are observed with both immunocytochemical approaches. In the interphase nucleolus, besides the labeling of the perinucleolar chromatin shell and of its intranucleolar invaginations which penetrate into the nucleolar body and often terminate at the fibrillar centers, a few gold particles are also preferentially found towards the peripheral ...
Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell edge or interior (CIL 34928) of cytochalasin D-treated Xenop...
The rapid growth of nanotechnology and its industries has elevated the need to understand the risks associated with handling, using, and disposing of nanomaterials
The rapid growth of nanotechnology and its industries has elevated the need to understand the risks associated with handling, using, and disposing of nanomaterials
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Genetics and Pathology. (Endocrine Pathology) ...
In this study we investigated the regulation of the activity of the vesicular monoamine transporters VMAT1 and VMAT2 by heterotrimeric G-proteins. In the human neuroendocrine cell line BON both transporters are expressed. They colocalize in these cells with the a-subunit of the heterotrimeric G-protein Go2 predominantely on Large Dense Core Vesicles (LDCVs). The activity of both VMAT1 and VMAT2 is regulated by Gao2. G-protein activation results in a down-regulation of vesicular monoamine uptake. VMAT2 appears to be more sensitive towards the observed G-protein regulation than VMAT1. Serotonergic raphe neurons in primary culture express VMAT2 as the neuronal form of the transporter. In these neurons VMAT2 predominantely localizes to Small Synaptic Vesicles (SSVs). Here, VMAT2 colocalizes with Gao2 on SSVs. In these neurons Gao2-dependent down-regulation of VMAT2 activity was observed, too. Immunoelectron microscopic analysis confirmed a localization of VMAT2 and Gao2 on SSVs from serotonergic ...
Two mammalian proteins, vti1a and vti1b, are homologous to the yeast Q-SNARE Vti1p which is part of several SNARE complexes in different transport steps. In vitro experiments suggest distinct functions for vti1a and vti1b. Here we compared the subcellular localization of endogenous vti1a and vti1b by immunofluorescence and immuno-electron microscopy. Both proteins had a distinct but overlapping localization. vti1a was found predominantly on the Golgi and the TGN, vti1b mostly on tubules and vesicles in the TGN area and on endosomes. vti1a coimmunoprecipitated with VAMP-4, syntaxin 6, and syntaxin 16. These four SNAREs could assemble into a SNARE complex of conserved structure because one SNARE motif of each subgroup is present. vtila-beta, VAMP-4, syntaxin 6, and syntaxin 16 are coenriched with small synaptic vesicles and with clathrin- coated vesicles isolated from rat brain synaptosomes. Therefore, this SNARE complex may have a role in synaptic vesicle biogenesis or recycling. ...
Part of the prestigious Max Planck Society based in Germany, MPFI is the first and only institute of its kind in North America. Situated in the new biosciences cluster in scenic Palm Beach County in South Florida, MPFI provides a vibrant, collaborative environment where scientists are provided generous ongoing support to conduct high impact research at the cutting edge.. ...
Introduction to microscopy techniques and their use in biology. Fluorescence microscopy, dyes, their characterization and use. Histochemical, immunohistochemical and immunofluorescence. Microscopic methods for detection of cell organelles ( nucleus, nucleolus, endoplasmic reticulum, Golgi apparatus, mitochondria, cytoskeleton, lysosomes, vacuoles and other membrane structures). Microscopic detection cell function and apoptosis (programmed cell death). Detection ions, reactive oxygen and nitrogen. Nanoparticles and their use in microscopic techniques. ...
Postdoctoral Position- Cell-Biology of polysaccharide Biosynthesis Complex Carbohydrate Research Center, University of Georgia Postdoctoral position is available to study the function of two gene families involved in the synthesis of nucleotide-sugars in plants. The project involves cellular and molecular biology methodology in addition to biochemistry and analysis wall mutants. Candidates must hold a Ph.D. in molecular biology or biochemistry with an excellent publication record and strong credentials in modern molecular biology/ genetics and enzymology/biochemistry. Experience with biochemical techniques such as heterologous protein expression, protein purification, enzymatic assays, antibody production, and experience with molecular and cellular techniques such as cloning, construction of fusion proteins (GFP), and immuno-EM protein localization is desirable. Salary commensurate with experience. Applicants should send a letter of interest, Curriculum vitae, selected reprints, and names, ...
Hugon, J and Borgers, M, "The ultrastructural localization of acid phosphatase in the crypt epithelium of the irradiated mouse duodenum." (1965). Subject Strain Bibliography 1965. 465 ...
Light and Electron Microscopic Evidence for a Direct Retinal Projection to the Pulvinar in the Asiatic Chipmunk,,I,Tamias sibricus,/I,. (1986 ...
Advanced optical imaging techniques can provide unprecedented details about the dynamics of life on a microscopic level. Such techniques typically rel
The epitope H contains an O-linked N-acetylglucosamine (O-GlcNAc) residue in a specific conformation and/or environment recognized by monoclonal antibody H (mAbH). We have previously shown that the epitope H is present in more than one polypeptide and in various types of normal and pathological cells. In the present study, we focused on mitochondria-rich normal, metaplastic, and neoplastic cells, prompted by our recent immuno-electron microscopy findings that mAbH clearly stains the mitochondria of breast epithelial cells in infiltrating ductal breast carcinomas and fibroadenomas. The indirect immunoperoxidase method was applied using the mAbH to investigate the distribution of the epitope H in mitochondria-rich normal cells and in metaplastic and neoplastic oncocytic cells. Immunohistochemical staining for the mAbH was observed in oxyphil cells of parathyroid glands, in striated duct cells of parotid glands, in urinary tubules of kidneys, in parietal cells of gastric body mucosa, in oxyphil ...
Morriss, Robert Harold. "The size and shape of colloidal gold particles." (1959) Diss., Rice University. https://hdl.handle.net/1911/18377 ...
Potassium channels are key determinants of neuronal excitability. We recently identified KChIPs as a family of calcium binding proteins that coassociate and colocalize with Kv4 family potassium channels in mammalian brain (An et al. [2000] Nature 403:553). Here, we used light microscopic immunohistochemistry and multilabel immunofluorescence labeling, together with transmission electron microscopic immunohistochemistry, to examine the subcellular distribution of KChIPs and Kv4 channels in adult rat cerebellum. Light microscopic immunohistochemistry was performed on 40-μm free-floating sections using a diaminobenzidine labeling procedure. Multilabel immunofluorescence staining was performed on free-floating sections and on 1-μm ultrathin cryosections. Electron microscopic immunohistochemistry was performed using an immunoperoxidase pre-embedding labeling procedure. By light microscopy, immunoperoxidase labeling showed that Kv4.2, Kv4.3, and KChIPs 1, 3, and 4 (but not KChIP2) were expressed at ...
The use of blochemical fractionation, immunofluorescence laser-scanning confocal microscopy, and immunoelectron microscopy with mouse anti-human bcl-2 monoclonal antibody to analyze the subcellular localization of the bcl-2 gene product revealed the protein prominently in the nuclear envelope, endoplasmic reticulum membrane, and mitochondrial membranes. Electron microscopy at high magnification more precisely localized bcl-2 to the nuclear outer membrane as confirmed by the biochemical fractionation, as well as to mitochondrial outer and, to a lesser degree, inner membrane. This multisite membrane distribution of bcl-2 suggests an important role for this protein in several different membrane compartments.. ...
This elegant study identifies Arc as a presenilin-1 (PS1) interacting protein that links synaptic activity with γ-secretase processing of APP in neurons. Detailed analysis of subcellular localization of APP, PS1, and Arc by immunofluorescence and immunoelectron microscopy methods suggest a mechanism whereby Arc facilitates association of PS1 with endosomes that contain internalized APP. Thus, it appears that the subset of APP that contributes to activity-dependent Aβ production encounters PS1 in endocytic organelles in dendrites of excitatory neurons.. Arc is known to associate with endophilin-2/3 and dynamin on endosomes, and mediate AMPA receptor (AMPAR) endocytosis. Whereas the last is markedly reduced in Arc knockout neurons, this does not appear to be the case with PS1 or APP. Whether this indicates Arc regulation of PS1/APP colocalization in tubulovesicular endosomes is distinct from its function in regulating the endocytic machinery responsible for AMPAR endocytosis remains to be ...
Release of merozoite dense granules during erythrocyte invasion by Plasmodium knowlesi.: We used immunoelectron microscopy to study the fate of dense granules d
Rhcg has a complex subcellular localization. Studies in the human, rat, and mouse kidney demonstrate that Rhcg-expressing cells exhibit both apical and basolateral Rhcg immunoreactivity, with the exception of the non-A, non-B intercalated cell, which has only apical Rhcg (17, 41, 53, 90, 91). Immunogold electron microscopy in both the rat and mouse kidney demonstrated that apical Rhcg is present in both the apical plasma membrane and subapical vesicles and that basolateral Rhcg is present in the basolateral plasma membrane (53, 91). Basolateral expression can be substantial; in the rat OMCD in the inner stripe, basolateral Rhcg is ∼25% of total cellular expression in intercalated cells and ∼40% in principal cells (91). Although initial studies in both the rat and mouse kidney did not identify basolateral Rhcg expression, more recent studies using improved immunohistochemistry techniques and a panel of anti-Rhcg antibodies confirmed basolateral Rhcg expression and demonstrated substantial ...
Ultrathin Sections, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 94/100, based on 547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Autozooids of the cheilostome bryozoan Aquiloniella scabracontain rod-like bacteria in the funicular bodies - the complex swellings of the funicular strands. Each funicular body contains symbionts...
In addition, mass spectrophotometry was executed to verify 442-51-3 detection of the LGp in C6/36 virions. Gel slices have been excised from protein separation