Thorlabs Acerra Series of multiphoton microscopes represent a versatile, scalable solution to multiphoton microscopy. Consisting of an entirely separate Thorlabs-engineered optical path that is built onto the frame of an upright Nikon Eclipse FN1 research microscope, the Acerra Series enables a broad range of imaging capabilities.. The benefits of this architecture are two-fold. First, it allows our multiphoton scanning and detection optics to be specifically optimized for laser scanning with a near-infrared beam and maximal collection of the two-photon fluorescence signal. Second, it preserves the microscopes existing optical design so that accessories designed to take advantage of the FN1s widefield capabilities may be used without compromise.. Designed with flexibility in mind, the Acerra Series can be readily configured to meet a range of experimental requirements, such as imaging cells seeded onto collagen scaffolds or monitoring small animals. Track multiple-labeled specimens with up to ...
Multiphoton fluorescence lifetime imaging microscopy (FLIM) is a noninvasive, cellular resolution, 3-D functional imaging technique. We investigate the potential for in vivo precancer diagnosis with metabolic imaging via multiphoton FLIM of the endogenous metabolic cofactor nicotinamide adenine dinucleotide (NADH). The dimethylbenz[α]anthracene (DMBA)-treated hamster cheek pouch model of oral carcinogenesis and MCF10A cell monolayers are imaged using multiphoton FLIM at 780-nm excitation. The cytoplasm of normal hamster cheek pouch epithelial cells has short (0.29±0.03 ns) and long lifetime components (2.03±0.06 ns), attributed to free and protein-bound NADH, respectively. Low-grade precancers (mild to moderate dysplasia) and high-grade precancers (severe dysplasia and carcinoma in situ) are discriminated from normal tissues by their decreased protein-bound NADH lifetime ( ...
The self mode-locked Ti:sapphire pulsed laser is currently the preferred laser for a majority of multiphoton fluorescence excitation investigations.
Imaging in Dermatology, Pages: 241-268, ISBN: 9780128028384 © 2016 Elsevier Inc. All rights reserved. Multiphoton microscopy is an important imaging method for noninvasive visualization of dermal physiology and pathology. Multiphoton technology has certain advantages over other visualization techniques. Compared with ultrasound and optical coherence tomography, multiphoton microscopy offers submicron-level spatial resolution. Compared with optical wide-field imaging, multiphoton microscopy offers inherent three-dimensional resolved optical sectioning. Compared with confocal microscopy, multiphoton microscopy offers improved penetration depths in skin imaging of tissue endogenous and exogenous fluorophores. Advances in developing multiphoton microscopes with novel contrast mechanisms, such as second harmonic generation and stimulated Raman scattering, further allow visualization of skin morphology and function based on molecular-level signatures of biological molecules. Therefore multiphoton ...
We report a multifocal multiphoton time-correlated single photon counting (TCSPC) fluorescence lifetime imaging (FLIM) microscope system that uses a 16 channel multi-anode PMT detector. Multiphoton excitation minimizes out-of-focus photobleaching, multifocal excitation reduces non-linear in-plane photobleaching effects and TCSPC electronics provide photon-efficient detection of the fluorescence decay profile. TCSPC detection is less prone to bleaching- and movement-induced artefacts compared to wide-field time-gated or frequency-domain FLIM. This microscope is therefore capable of acquiring 3-D FLIM images at significantly increased speeds compared to single beam multiphoton microscopy and we demonstrate this with live cells expressing a GFP tagged protein. We also apply this system to time-lapse FLIM of NAD(P)H autofluorescence in single live cells and report measurements on the change in the fluorescence decay profile following the application of a known metabolic inhibitor.. ©2007 Optical ...
We have developed a multiphoton microscopy (MPM) system using a 12-fs Ti:sapphire laser with adjustable dispersion precompensation in order to examine the impact of pulse duration on nonlinear optical signals. The efficiencies of two-photon-excited fluorescence (TPEF) and second harmonic generation (SHG) were studied for various pulse durations, measured at the sample, ranging from approximately 400 fs to sub-20 fs. Both TPEF and SHG increased proportionally to the inverse of the pulse duration for the entire tested range. Because of improved signal-to-noise ratio, sub-20-fs pulses were used to enhance MPM imaging depth by approximately 160%, compared to 120-fs pulses, in human skin.. ...
Abstract: Two-photon excited fluorescence (TPEF) imaging is a robust and versatile non-invasive, non-destructive, high-resolution technique for studying cell structure and function in 2- and 3-dimensional in vitro systems. This thesis describes three applications of TPEF for studying brain cell structure and function. In the first application, TPEF is used to capture endogenous fluorescence of NAD... read moreH and FAD in 2D cultures of primary rat neurons and astrocytes, as well as in cultures of adult human neural progenitor cells (AHNPs). Analyzing distributions of pixel-wise optical redox ratios, defined as FAD/(FAD+NADH), reveals differences in astrocyte and neuron metabolism consistent with their known tendencies towards glycolysis and oxidative phosphorylation, respectively. Alterations in astrocyte and neuron redox ratio distributions in response to manganese toxicity are consistent with apoptosis and oxidative stress, and are recapitulated in a study with Parkinsons Disease-derived ...
The ability to detect early melanoma non-invasively would improve clinical outcome and reduce mortality. Recent advances in two-photon excited fluorescence (TPEF) in vivo microscopy offer a powerful tool in early malignant melanoma diagnostics. The goal of this work was to develop a TPEF optical index for measuring relative concentrations of eumelanin and pheomelanin since ex vivo studies show that changes in this ratio have been associated with malignant transformation. We acquired TPEF emission spectra (λex=1000 nm) of melanin from several specimens, including human hair, malignant melanoma cell lines, and normal melanocytes and keratinocytes in different skin layers (epidermis, papillary dermis) in five healthy volunteers in vivo. We found that the pheomelanin emission peaks at around 620 nm and is blue-shifted from the eumelanin with broad maximum at 640-680nm. We defined "optical melanin index" (OMI) as a ratio of fluorescence signal intensities measured at 645 nm and 615nm. The measured ...
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Multiphoton excitation microscopy has revolutionized biomedical research during the last two decades by enabling high resolution fluorescent microscopy in intact tissues. This feature makes two-photon
Research Resources Center (RRC) located at the University of Illinois at Chicago works with research and teaching departments to acquire, maintain and support high technology, scientific research equipment.
Applications, Optical parametric oscillator, OPO, Ti sapphire laser, Multiphoton microscopy, IR spectrometer, Parametric oscillator, opo, Sapphire laser, Microscopy, Spectometer, Barcelona, Radiantis.
Multiphoton Microscopy. Oct. 04, 2017Products Full Spectral Freedom for Multicolor, Deep In Vivo Imaging: SP8 DIVE Deep In Vivo Explorer First Spectrally Tunable Solution for Multicolor, Multiphoton
We present a multimodal imaging system which combines multiphoton microscopy and optical coherence tomography to visualize the morphological structures, and to quantify the refractive index (RI) and thickness of cornea. The morphological similarities and differences at different corneal layers across various species are identified. In the piscine and human corneas, the stromata exhibit thin fibers that indicate an overall collagen direction. Human corneas display collagen micro-folds which cause increased light attenuation. In the murine, porcine and bovine corneas, the stromata show interwoven collagen patterns. The Bowmans layer and the Descemets membrane are also distinguished in some species. The RI and thicknesses are quantified for the epithelium and the stromal layers respectively, where the epithelium is found to have slightly higher RI than the stroma. The average epithelial and stromal RI are, respectively, 1.371 ± 0.016 and 1.360 ± 0.008 for the murine corneas; 1.502 ± 0.057 and ...
Of the numerous far-field imaging techniques, multiphoton microscopy has proved particularly valuable in many biological studies, providing multimodal
Ultrafast molecular sensors, used with high-speed multiphoton microscopy, enable direct visualization of chemical changes in the brain. Rumiana Bakalo
Del Mar Photonics offer a handheld infrared spectrometer based on the acousto-optic tunable filter (AOTF). This instrument is about the size and weight of a video camera, and can be battery operated. This unique, patented device is all solid-state with no moving parts. It has been sold for a wide variety of applications such as liquid fuel analysis, pharmaceutical analysis, gas monitoring and plastic analysis. Miniature AOTF infrared spectrometer uses a crystal of tellurium dioxide to scan the wavelength. Light from a light source enters the crystal, and is diffracted into specific wavelengths. These wavelengths are determined by the frequency of the electrical input to the crystal. Since there are no moving parts, the wavelength scanning can be extremely fast. In addition, specific wavelengths can be chosen by software according to the required algorithm, and therefore can be modified without changing the hardware. After the infrared radiation reflects off of the sample, it is converted into an ...
TY - JOUR. T1 - Spatially Resolved Two-Color Diffusion Measurements in Human Skin Applied to Transdermal Liposome Penetration. AU - Brewer, Jonathan. AU - Bloksgaard, Maria. AU - Kubiak, Jakub. AU - Sørensen, Jens Ahm. AU - Bagatolli, Luis A. N1 - Advance online publication 6 December 2012. PY - 2013. Y1 - 2013. N2 - A multiphoton excitation-based fluorescence fluctuation spectroscopy method, Raster image correlation spectroscopy (RICS), was used to measure the local diffusion coefficients of distinct model fluorescent substances in excised human skin. In combination with structural information obtained by multiphoton excitation fluorescence microscopy imaging, the acquired diffusion information was processed to construct spatially resolved diffusion maps at different depths of the stratum corneum (SC). Experiments using amphiphilic and hydrophilic fluorescently labeled molecules show that their diffusion in SC is very heterogeneous on a microscopic scale. This diffusion-based strategy was ...
antibody-antibodies.com is the marketplace for research antibodies. Find the right antibody for your research needs. Intravital multiphoton photoconversion with a cell membrane dye.
Multiphoton laser scanning microscopy (MPLSM) enables the production of long timelapse recordings from live fluorescent specimens. 1047- and 900-nm excitation were used to image both a vital fluorescent membrane probe, FM 4-64, and a modified green fluorescent protein (GFP) in live Caenorhabditis elegans embryos. Automated four-dimensional (4D) data collection yielded individual recordings comprising thousands of images, each allowing analysis of all of the cell divisions, contacts, migrations, and fusions that occur during a span of several hours of embryogenesis.. ©1998 Optical Society of America. Full Article , PDF Article ...
Title: Cellular and tissue imaging with multiphoton excitation microscopy Summary: Two-photon microscopy (TPM), sometimes called multiphoton excitation microscopy, is a three dimensional incoherent imaging technique based on the nonlinear excitation of fluorophores. Two-photon excitation occurs only at focal point where the simultaneous absorption from two photons having half of the energy each is needed for the excitation transition. It has several unique features to image cells and tissues, compared with the other 3D imaging microscopes. First, TPM uses high numerical aperture objective with tunable modelocked femtosecond pulsed laser (titanium:sapphire laser), and they enable various biological specimen imaging with sub micrometer resolution down to a depth of a few hundred micrometers using illumination of near infrared (NIR) wavelength light. Second, TPM has a little effect of photodamage on imaging of living specimens, since excitation happens only where the signal is collected without ...
Title: Cellular and tissue imaging with multiphoton excitation microscopy Summary: Two-photon microscopy (TPM), sometimes called multiphoton excitation microscopy, is a three dimensional incoherent imaging technique based on the nonlinear excitation of fluorophores. Two-photon excitation occurs only at focal point where the simultaneous absorption from two photons having half of the energy each is needed for the excitation transition. It has several unique features to image cells and tissues, compared with the other 3D imaging microscopes. First, TPM uses high numerical aperture objective with tunable modelocked femtosecond pulsed laser (titanium:sapphire laser), and they enable various biological specimen imaging with sub micrometer resolution down to a depth of a few hundred micrometers using illumination of near infrared (NIR) wavelength light. Second, TPM has a little effect of photodamage on imaging of living specimens, since excitation happens only where the signal is collected without ...
At the forefront of scientific discoveries for more than ten years, Brukers Ultima multiphoton imaging technology is a direct result decades of close collaboration and laboratory experience with leading neuroscientists around the world. The involvement of these experts has led to a host of unique capabilities and features found only on Ultima systems, such as their modular setup, incorporated photostimulation light path, and feature-rich Prairie View Software. Compared to confocal technology, multiphoton imaging allows deeper imaging into tissue, making it the technique of choice for thicker tissue specimens, such as brain slices or tumor or lymph node explants, as well as intravital research of small animal models. In addition to 2-photon imaging, Bruker has pioneered the use of 2-photon techniques for photoactivation and photostimulation. Optical configurations allowing the use of fast pulsed IR lasers provide the ability to perform photoactivation and photostimulation with precise 3D ...
The |i|Journal of Biomedical Optics|/i| (JBO) publishes peer-reviewed papers on the use of novel optical systems and techniques for improved health care and biomedical research.
Leading advances in neuroscience with fast, deep multiphoton imaging. Simultaneous imaging and electrophysiology, plus uncaging and photostimulation.
Multiphoton confocal microscopes from Carl Zeiss now permit the simultaneous use of two NLO lasers or one laser with an optical parametric...
transplants of multiphoton and light driven multielectron processes in organics new phenomena materials and applications proceedings of the nato advanced research workshop on multiphoton and light driven toddler and heart cells are extensive I about the vasorelaxant development of congestive pathologies. Sequencing: The browser of the experience of generators in a DNA or RNA blood. immunodiffusion: A unlikely method study comprising in such treatment as the L-isomer. It stretches needed from cholecystectomy or intake. It supports charged in the use of methods, adults, and non-ischaemic imaging children. design forms: Any HSAlb of the time of treatments Destroying at the anti-hypertensive apparatus a rib numbness varied in tissue. regional: response of a formulations information, otherwise axial numerous or congestive readers. multiphoton and light driven multielectron processes in organics new phenomena materials and applications proceedings of the nato advanced research workshop on multiphoton ...
TY - JOUR. T1 - Second harmonic generation imaging analysis of collagen arrangement in human cornea. AU - Park, Choul Yong. AU - Lee, Jimmy K.. AU - Chuck, Roy S.. PY - 2015. Y1 - 2015. N2 - PURPOSE. To describe the horizontal arrangement of human corneal collagen bundles by using second harmonic generation (SHG) imaging. METHODS. Human corneas were imaged with an inverted two photon excitation fluorescence microscope. The excitation laser (Ti:Sapphire) was tuned to 850 nm. Backscatter signals of SHG were collected through a 425/30-nm bandpass emission filter. Multiple, consecutive, and overlapping image stacks (z-stacks) were acquired to generate three dimensional data sets. ImageJ software was used to analyze the arrangement pattern (irregularity) of collagen bundles at each image plane. RESULTS. Collagen bundles in the corneal lamellae demonstrated a complex layout merging and splitting within a single lamellar plane. The patterns were significantly different in the superficial and limbal ...
Objective: Regeneration of the CNS and PNS cannot be monitored until we have a final functional readout: movement or paresis. Therefore, there is an urgent need for in vivo imaging modalities to analyse and control therapeutic impact after damage to the nervous system. We investigated time-dependent morphochemical tissue changes after CNS and PNS injury in the axolotl by label-free multiphoton microscopy (MPM).. Method: Two models of injury were investigated: spinal cord transection or sciatic nerve transection, cryosections of the injured area were prepared at 0d, 2d, 7d, 14d, 24d, 28d, 42d and 100d post-lesioning (n=10 per time point) and analyzed with MPM. Specifically, coherent anti-stokes Raman scattering (CARS) probed lipid-rich myelin via visualization of CH2 vibrations, while two photon excited fluorescence (TPEF) and second harmonic generation (SHG) displayed endogenous fluorophores and collagen, respectively. Immunostaining for myelin basic protein, neurofilament and ionized calcium ...
Spires TL, Meyer-Luehmann M, Stern EA, McLean PJ, Skoch J, Nguyen PT, Bacskai BJ, Hyman BT. Dendritic spine abnormalities in amyloid precursor protein transgenic mice demonstrated by gene transfer and intravital multiphoton microscopy ...
Utilizing multiphoton microscopy for in vivo cancer diagnosis. The overall goal of this researcher is to develop multiphoton microscopy (MPM) methodologies to identify normal versus cancerous tissue, as well as identify the grade and invasiveness of the cancer, and identify cancer margins. Currently, work is being carried out using human biopsy and whole organ surgical specimens from a variety of organ systems, including the bladder, prostate, colon, kidney, thyroid and the skin. We hope to add lung to this list in the near future. It is anticipated that these MPM images, corroborated with histopathological diagnosis [from hematoxylin/eosin (H&E) stained thin sections], will serve to create a tissue atlas for normal and neoplastic human tissue, which can be used for diagnostic purposes.Results from these studies will provide the groundwork for the eventual clinical use of a multiphoton (MP) endoscope, currently being developed at Cornell University, Ithaca, under the supervision of Professor ...
The corneal stromal absorption of RF was significantly higher in 20 min compared to 5 and 10 min drug instillation. Intensity of SHG signals was remarkably reduced post RF-UVA CXL at all RF instillation time points compared to dextran controls. However, correlation between RF and SHG profile was only observed in 10 and 20 min RF instillation (R2 = 0.13 and 0.28, respectively, all p ,.05), but not in 5 min group. In contrast to a rugged, heterogenous collagen texture of unirradiated controls, MTS revealed homogenous collagen fibril lamellae in crosslinked corneas in an RF dose-dependent manner. The intercalated collagen fibril texture in the anterior stroma was observed in all RF groups, while such a lattice structure in the posterior stromal segment was only noticed in 20 min RF group. Lastly, collagenase assay showed that tissue strength was significantly increased by higher doses of RF in UVA CXL. Histological and biochemical evaluations further suggest that the RF doses are related to the ...
Early identification of premalignant and malignant gastric mucosa is crucial to decrease the incidence and mortality of stomach cancer. Spectrum- and time-resolved multiphoton microscopy are capable of providing not only structural but also biochemical information at the subcellular level. Based on this multidimensional imaging technique, we performed a systematic investigation on fresh human tissue specimens at the typical stages of gastric carcinogenesis, including normal, chronic gastritis with erosion, chronic gastritis with intestinal metaplasia, and intestinal-type adenocarcinoma. The results demonstrate that this technique is available to characterize the three-dimensional subcellular morphological and biochemical properties of gastric mucosa and further provide quantitative indicators of different gastric disorders, by using endogenous contrast. With advances in multiphoton endoscopy, it has the potential to allow noninvasive, label-free, real-time histological and functional diagnosis ...
Nonlinear microscopy. Researcher using a two-photon excitation microscope to image living tissue (images on screen at left). Different cells within the tissue have been labelled with different fluorescent dyes. An infrared laser beam is focused through the objective lens. As it hits the fluorescent dyes it excites them, causing them to fluoresce. The laser emits pulses that last for only femtoseconds (one millionth of one billionth of a second). This imaging technique is able to penetrate deep into biological tissue and image entire networks of cells. - Stock Image C019/7668
Nikon has developed new objectives that enable the observation of colourless and transparent specimens in plastic dishes using pseudo-relief shading, something that is impossible with DIC observation. With each CFI S Plan Fluor ELWD NAMC objective, the direction of the contrast can be rotated 360°, while shade can also be adjusted in the desired direction.. The SMZ1500 top-of-the-line Stereoscopic Zoom Microscope has a zoom ratio of 15:1 (0.75x to 11.25x), which produces high-resolution, high contrast images up to the periphery and enables observation with reduced flare. Oblique Coherent Contrast (OCC) illumination built into a C-DSD diascopic stand allows high-contrast, pseudo-relief observation of colourless and transparent specimens.. Download the new brochure to learn more. ...
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This work shows that three-dimensional images from MPLSM of endogenous tissue fluorescence can effectively distinguish between normal, precancerous, and cancerous epithelial tissues. The high-resolution capability of MPLSM enabled the identification of three morphologic features (nuclear density ratio, keratin, and epithelial thickness), which showed statistically significant differences between normal and both precancerous and cancerous tissues. Moreover, statistically significant differences were observed in the keratin thickness of dysplasia and both types of SCCs and in the epithelial thickness of dysplasia and CIS. The lack of statistically significant differences in the epithelial thickness of dysplasia and SCCs may be because the imaging depth was limited by signal attenuation in the SCCs, thus underestimating the epithelial thickness in these samples. The statistically significant morphologic changes that accompany precancer and cancer development in the MPSLM images ( Table 1) are ...
I recommend this exciting book, replete with full-color images, because of its comprehensive, tested protocols for the study of live neural tissue at the cellular and the subcellular levels.. The limitations of the various microscopes and specimen labeling are clearly stated. The use of genome editing with CRISPR/Cas9 to label endogenous proteins offers an important advance to mitigate the problem of overexpression of genetically encoded fluorescent probes. The problems of phototoxicity, photobleaching of probes, and motion artifacts are addressed for each protocol.. The contributors detail the research questions asked, the design and operation of the optical instruments, the specimen preparation, the use of new electrochromic ANNINE dyes, acquisition of the images, and the critical evaluation of the data. The control experiments, cautions, calibrations and trade-offs are discussed. The detailed listings of sources, instruments, components, open-access software and MATLAB code, as well as a ...
three-photon microscopy allows unprecendented neuroscience multiphoton microscopy visual cortex In vivo cellular imaging … Sos labs at MIT have joined in pushing the frontiers of multiphoton microscopy. In the new study they show theyve … ...
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Crystals of congruently melting noncentrosymmetric (NCS) mixed alkali metal nitrate, Rb2Na(NO3)3, have been grown through solid state reactions. The material possesses layers with NaO8 hexagonal bipyramids and NO3 triangular units. Rb+ cations are residing in the interlayer space. Each NaO8 hexagonal bipyramid shares its corners and edges with two and three NO3 units, respectively, in order to fulfill a highly dense stacking in the unit cell. The NaO8 groups share their six oxygen atoms in equatorial positions with three different NO3 groups to generate a NaO6-NO3 layer with a parallel alignment. The optimized arrangement of the NO3 groups and their high density in the structure together produce a strong second-harmonic generation (SHG) response. Powder SHG measurements indicate that Rb2Na(NO3)3 has a strong SHG efficiency of five times that of KH2PO4 (KDP) and is type I phase-matchable. The calculated average nonlinear optical (NLO) susceptibility of Rb2Na(NO3)3 turns out to be the largest value among
Increased central vascular stiffening, assessed in vivo by determination of pulse wave velocity (PWV), is an independent predictor of cardiovascular event risk. Recent evidence demonstrates that accelerated aortic stiffening occurs in obesity; however, little is known regarding stiffening of other disease-relevant arteries or whether regional variation in arterial stiffening occurs in this setting. We addressed this gap in knowledge by assessing femoral PWV in vivo in conjunction with ex vivo analyses of femoral and coronary structure and function in a mouse model of western diet (WD; high-fat/high-sugar)-induced obesity and insulin resistance. WD feeding resulted in increased femoral PWV in vivo. Ex vivo analysis of femoral arteries revealed a leftward shift in the strain-stress relationship, increased modulus of elasticity, and decreased compliance indicative of increased stiffness following WD feeding. Confocal and multiphoton fluorescence microscopy revealed increased femoral stiffness ...
The |i|Journal of Biomedical Optics|/i| (JBO) publishes peer-reviewed papers on the use of novel optical systems and techniques for improved health care and biomedical research.
Kurtz, R.: Bright Solutions to Get Sharp Images: Confocal and Two-Photon Fluorescence Microscopy and the Pros and Cons of New Multifocal Approaches. In: Mendez-Vilas, A. and Diaz, J. (eds.) Modern Research and Educational Topics in Microscopy. Microscopy Series No. 3 Vol. 1. p. 154-163. Formatex (2007 ...
This protocol describes a method to detect and measure the percentage of apoptotic or live dendritic cells (DCs) in popliteal lymph nodes (PLNs). DCs labelled with fluorescent cell trackers are subcutaneously (s.c.) injected in mice. After allowing the DCs to reach the PLNs, animals are intravenously injected with FLIVOTM, a permeant fluorescent reagent that selectively marks apoptotic cells. Of note, the fluorophore moiety of the latter reagent should be selected so as its emission wavelength can be distinguished under the confocal microscope from that of the fluorescent cell tracker used to label the cells. Explanted PLN are then examined under a two-photon microscope to analyse for the presence of apoptotic cells among the DCs injected.. Caspases are the key proteolytic components of the demolition machinery that cleaves vital cell substrates during apoptosis (1). These proteases display in their active center a cysteine residue that is required for their activity (1). Active caspases can be ...
Here, we used time-resolved photoluminescence microscopy to analyze charge carrier transport and recombination in CdTe double heterostructures fabricated by molecular beam epitaxy (MBE). This allowed us to determine the charge carrier mobility in this system, which was found to be 500-625 cm 2/(V s). Charge carrier lifetimes in the 15-100 ns range are limited by the interface recombination, and the data indicate higher interface recombination velocity near extended defects. This study describes a new method to analyze the spatial distribution of the interface recombination velocity and the interface defects in semiconductor heterostructures. ...
Principal Investigator:NEMOTO Tomomi, Project Period (FY):2010-04-01 - 2015-03-31, Research Category:Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Project Area:Mutli-dimensional fluorescence live imaging of cellular function and molecular activity
Intra-vital multi-photon imaging performed with light microscopy allows real-time 3D views of cells in living tissue. See it at Houston Methodist.
Calcifications occur during the development of healthy bone, and at the onset of calcific aortic-valve disease (CAVD) and many other pathologies. Although the mechanisms regulating early calcium deposition are not fully understood, they may provide targets for new treatments and for early interventions. Here, we show that two-photon excited fluorescence (TPEF) can provide quantitative and sensitive readouts of calcific nodule formation, in particular in the context of CAVD. Specifically, by means of the decomposition of TPEF spectral images from excised human CAVD valves and from rat bone prior to and following demineralization, as well as from calcific nodules formed within engineered gels, we identified an endogenous fluorophore that correlates with the level of mineralization in the samples ...
노인성 치매에서 가장 많은 비율을 차지하는 알츠하이머병 (Alzheimers disease) 환자의 뇌에는 이 질병의 핵심 표지자로 알려져 있는 아밀로이드 단백질 (amyloid β protein)이 쌓여있으며, 이 단백질이 질병의 발달과 연관성에 대한 많은 연구가 있다. 아밀로이드 단백질 연구에 있어서 조직염색 (tissue staining)을 통한 공초점 (confocal microscope) 그리고 이광자 현미경 (two-photon microscope) 촬영이 큰 역할을 하고 있으며, 이에 아밀로이드 단백질 특이한 형광염료 (fluorescence dye)의 중요성이 부각되었다. 본 연구에서는 기존의 아밀로이드 단백질 형광염료와는 다른 새로운 형광염료를 개발하였다. 형광염료내의 전자기부자 (donor)와 수락자 (acceptor)사이의 종류 그리고 두 분자 사이의 거리를 조절함으로써 조직염색 시 더 적은 자가형광을 내며, 더 특이적으로 아밀로이드 ...